Isomaltooligosacharide gel for preventing and treating gynecological genital tract inflammation and preparation method of isomaltooligosacharide gel

Abstract

The invention belongs to the technical field of health care, and particularly relates to isomaltooligosaccharide gel for preventing and treating gynecological genital tract inflammation and a preparation method of the isomaltooligosaccharide gel. The invention provides an isomaltooligosacharide gel for preventing and treating gynecological genital tract inflammation. The isomaltooligosacharide gel comprises isomaltooligosacharide, carbomer, gelatin, glycerol, triethanolamine, benzyl alcohol, phenoxyethanol and water. According to the invention, the isomaltooligosacharide, the carbomer and other components are specifically combined, so that the isomaltooligosacharide can maintain normal reproduction of vaginal probiotics. Therefore, when the isomaltooligosacharide gel provided by the invention is applied to the products for preventing and treating the gynecological genital tract inflammation, the occurrence of the gynecological genital tract inflammation can be reduced, red, swollen and pruritus can be quickly relieved, and pain can be reduced.

Description

TECHNICAL FIELD

[0001] The present application belongs to the field of health care, and particularly relates to an isomalto-oligosaccharide gel for preventing and treating gynecological inflammation and a preparation method thereof. BACKGROUND

[0002] The vagina is one of the important body organs of a female, which plays a variety of important physiological roles in the female body. However, many women are plagued by gynecological diseases such as cervical erosion, ulcer, vaginitis, etc. Common ways to treat the above-mentioned diseases include drug therapy and surgical therapy. Surgical therapy is relatively quick, but often brings inconvenience to patients in life and physiology. Common drug dosage forms for drug therapy include effervescent tablets, suppositories and flushing liquids, but drug therapy is slow in effect and inconvenient to use.

[0003] In addition, since gynecological inflammation does not show corresponding inflammation in advance, it is difficult to prevent, so existing products are mostly for treating patients who have already been ill, and products for preventing gynecological inflammation are very few. Therefore, how to provide a product which is effective, safe and convenient, and can not only be used for treatment, but also can prevent gynecological inflammation is a difficult problem to be solved in the field. SUMMARY

[0004] The present application aims to provide an isomalto-oligosaccharide gel for preventing and treating gynecological inflammation and a preparation method thereof, which has the characteristics of fast effect, safety and convenience.

[0005] The present application provides an isomalto-oligosaccharide gel for preventing and treating gynecological inflammation, which comprises, by mass percentage, 4%-6% of isomalto-oligosaccharide, 0.3%-0.5% of carbomer, 0.1-0.3% of gelatin, 1.5%-2.5% of glycerol, 0.05%-0.08% of triethanolamine, 0.1%-0.3% of benzyl alcohol, 0.1%-0.5% of phenoxyethanol and the balance of water.

[0006] Preferably, the mass concentration of isomaltulose and panose in the isomalto-oligosaccharide is greater than or equal to 50%.

[0007] Preferably, the carbomer comprises carbomer 941 and carbomer 940.

[0008] Preferably, the mass ratio of the carbomer 941 and the carbomer 940 is 1:1-1.5.

[0009] Preferably, the water comprises purified water.

[0010] The present application provides a preparation method of the isomalto-oligosaccharide gel, comprising the following steps: stirring the water, the carbomer and the gelatin uniformly to obtain a mixture; The oligoisomaltose, glycerol, triethanolamine, benzyl alcohol and phenoxyethanol are sequentially added into the mixture to obtain the oligoisomaltose gel.

[0011] The application provides application of the oligoisomaltose gel in preparation of a product for preventing and / or treating gynecological reproductive tract inflammation.

[0012] Preferably, the gynecological reproductive tract inflammation includes one or more of redness, itching and pain.

[0013] Beneficial effects: The application provides an oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, which contains, in percentage by mass, 4%-6% of oligoisomaltose, 0.3%-0.5% of carbomer, 0.1-0.3% of gelatin, 1.5%-2.5% of glycerol, 0.05%-0.08% of triethanolamine, 0.1%-0.3% of benzyl alcohol, 0.1%-0.5% of phenoxyethanol and the rest of water. In the oligoisomaltose gel, the oligoisomaltose is combined with other substances to promote the release of the function of regulating flora of the oligoisomaltose, help maintain the self-purification of the vaginal environment, and thus help the normal reproduction of the self-beneficial bacteria in the vagina, reduce gynecological reproductive tract inflammation and promote the health of the reproductive system. In addition, the addition of carbomer and glycerol improves the practicability of the oligoisomaltose gel and helps ensure smooth use of the product and reduce the pain of patients.

[0014] Based on the above technical advantages, the application also provides application of the oligoisomaltose gel in preparation of a product for preventing and / or treating gynecological reproductive tract inflammation. The oligoisomaltose gel is applied to the product for preventing and / or treating gynecological reproductive tract inflammation, which not only reduces the occurrence of gynecological reproductive tract inflammation but also quickly reduces redness, itching and pain. DETAILED DESCRIPTION

[0015] In the application, the reagents and equipment used are conventionally selected unless otherwise specified.

[0016] In order to further illustrate the application, the application is described in detail below in combination with examples, but they should not be understood as limiting the protection scope of the application.

[0017] Example 1 A preparation method of an oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, comprising the following steps: (1) mixing carbomer 941 and carbomer 940 according to a mass ratio of 1:1 to obtain carbomer for standby use; (2) Selecting oligoisomaltose with mass concentration of isomaltose and panose ≥ 50% for standby; (3) Oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, comprising, in mass percentage, oligoisomaltose 5%, carbomer 0.4%, gelatin 0.1%, glycerol 2%, triethanolamine 0.07%, benzyl alcohol 0.1%, phenoxyethanol 0.1%, and the balance of purified water; (4) Specific preparation process: According to the percentage content in step (3), the water is uniformly stirred with the carbomer and the gelatin to obtain a mixture; then the oligoisomaltose, the glycerol, the triethanolamine, the benzyl alcohol, and the phenoxyethanol are sequentially added to the mixture for sufficient dissolution, and then the uniform oligoisomaltose gel system is obtained.

[0018] Example 2 A preparation method of oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, comprising the following steps: (1) Mixing carbomer 941 and carbomer 940 according to a mass ratio of 1:1 to obtain carbomer for standby; (2) Selecting oligoisomaltose with mass concentration of isomaltose and panose ≥ 50% for standby; (3) Oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, comprising, in mass percentage, oligoisomaltose 6%, carbomer 0.4%, gelatin 0.1%, glycerol 2%, triethanolamine 0.07%, benzyl alcohol 0.1%, phenoxyethanol 0.1%, and the balance of purified water; (4) Specific preparation process: According to the percentage content in step (3), the water is uniformly stirred with the carbomer and the gelatin to obtain a mixture; then the oligoisomaltose, the glycerol, the triethanolamine, the benzyl alcohol, and the phenoxyethanol are sequentially added to the mixture for sufficient dissolution, and then the uniform oligoisomaltose gel system is obtained.

[0019] Example 3 A preparation method of oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, comprising the following steps: (1) Mixing carbomer 941 and carbomer 940 according to a mass ratio of 1:1 to obtain carbomer for standby; (2) Selecting oligoisomaltose with mass concentration of isomaltose and panose ≥ 50% for standby; (3) Oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, comprising, in mass percentage, oligoisomaltose 5%, carbomer 0.4%, gelatin 0.1%, glycerol 2%, triethanolamine 0.05%, benzyl alcohol 0.1%, phenoxyethanol 0.1%, and the balance of purified water; (4) The specific preparation process is: According to the percentage in step (3), water is uniformly stirred with carbomer and gelatin to obtain a mixture; then oligoisomaltose, glycerol, triethanolamine, benzyl alcohol and phenoxyethanol are sequentially added to the mixture for sufficient dissolution, and then the system is uniformly oligoisomaltose gel.

[0020] Comparative Example 1 A preparation method of oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, the steps are: (1) only using carbomer 941, standby; (2) selecting oligoisomaltose with mass concentration of isomaltose and panose ≥ 50%, standby; (3) oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, including: oligoisomaltose 5%, carbomer 0.4%, gelatin 0.1%, glycerol 2%, triethanolamine 0.07%, benzyl alcohol 0.1%, phenoxyethanol 0.1% and the balance of purified water, in terms of mass percentage; (4) The specific preparation process is: According to the percentage in step (3), water is uniformly stirred with carbomer and gelatin to obtain a mixture; then oligoisomaltose, glycerol, triethanolamine, benzyl alcohol and phenoxyethanol are sequentially added to the mixture for sufficient dissolution, and then the system is uniformly oligoisomaltose gel.

[0021] Comparative Example 2 A preparation method of oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, the steps are: (1) mixing carbomer 941 and carbomer 940 according to a mass ratio of 3:1 to obtain carbomer, standby; (2) selecting oligoisomaltose with mass concentration of isomaltose and panose ≥ 50%, standby; (3) oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, including: oligoisomaltose 5%, carbomer 0.4%, gelatin 0.1%, glycerol 2%, triethanolamine 0.07%, benzyl alcohol 0.1%, phenoxyethanol 0.1% and the balance of purified water, in terms of mass percentage; (4) The specific preparation process is: According to the percentage in step (3), water is uniformly stirred with carbomer and gelatin to obtain a mixture; then oligoisomaltose, glycerol, triethanolamine, benzyl alcohol and phenoxyethanol are sequentially added to the mixture for sufficient dissolution, and then the system is uniformly oligoisomaltose gel.

[0022] Comparative Example 3 A preparation method of an isomalto-oligosaccharide gel for preventing and treating inflammation of the gynecological genital tract, comprising the following steps: (1) mixing carbomer 941 and carbomer 940 according to a mass ratio of 1:3 to obtain carbomer, for standby; (2) selecting isomalto-oligosaccharide with a mass concentration of isomalt and panose of greater than or equal to 50% for standby; (3) the isomalto-oligosaccharide gel for preventing and treating inflammation of the gynecological genital tract comprises, in percentage by mass, 5% of isomalto-oligosaccharide, 0.4% of carbomer, 0.1% of gelatin, 2% of glycerol, 0.07% of triethanolamine, 0.1% of benzyl alcohol, 0.1% of phenoxyethanol, and a balance of purified water; (4) a specific preparation process: according to the percentage content in step (3), stirring water, carbomer and gelatin uniformly to obtain a mixture; then sequentially adding isomalto-oligosaccharide, glycerol, triethanolamine, benzyl alcohol and phenoxyethanol into the mixture for sufficient dissolution, and then obtaining a uniform isomalto-oligosaccharide gel system.

[0023] Comparative Example 4 A preparation method of an isomalto-oligosaccharide gel for preventing and treating inflammation of the gynecological genital tract, comprising the following steps: (1) only using carbomer 940 for standby; (2) selecting isomalto-oligosaccharide with a mass concentration of isomalt and panose of greater than or equal to 50% for standby; (3) the isomalto-oligosaccharide gel for preventing and treating inflammation of the gynecological genital tract comprises, in percentage by mass, 5% of isomalto-oligosaccharide, 0.4% of carbomer, 0.1% of gelatin, 2% of glycerol, 0.07% of triethanolamine, 0.1% of benzyl alcohol, 0.1% of phenoxyethanol, and a balance of purified water; (4) a specific preparation process: according to the percentage content in step (3), stirring water, carbomer and gelatin uniformly to obtain a mixture; then sequentially adding isomalto-oligosaccharide, glycerol, triethanolamine, benzyl alcohol and phenoxyethanol into the mixture for sufficient dissolution, and then obtaining a uniform isomalto-oligosaccharide gel system.

[0024] Comparative Example 5 A preparation method of an isomalto-oligosaccharide gel for preventing and treating inflammation of the gynecological genital tract, comprising the following steps: (1) mixing carbomer 941 and carbomer 940 according to a mass ratio of 1:1 to obtain carbomer, for standby; (2) selecting isomalto-oligosaccharide with a mass concentration of isomalt and panose of greater than or equal to 50% for standby; (3) The oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation comprises, by mass percentage, oligoisomaltose 10%, carbomer 0.4%, gelatin 0.1%, glycerol 2%, triethanolamine 0.07%, benzyl alcohol 0.1%, phenoxyethanol 0.1%, and the balance of purified water; (4) The specific preparation process is as follows: According to the percentage in step (3), the water is uniformly stirred with the carbomer and the gelatin to obtain a mixture; then the oligoisomaltose, the glycerol, the triethanolamine, the benzyl alcohol, and the phenoxyethanol are sequentially added to the mixture for sufficient dissolution, and then the uniform oligoisomaltose gel system is obtained.

[0025] Comparative Example 6 A preparation method of an oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, the steps are as follows: (1) The carbomer 941 and the carbomer 940 are mixed according to a mass ratio of 1:1 to obtain a carbomer for standby use; (2) The oligoisomaltose with a mass concentration of <50% of isomaltulose and panose is selected for standby use.

[0026] (3) The oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation comprises, by mass percentage, oligoisomaltose 5%, carbomer 0.4%, gelatin 0.1%, glycerol 2%, triethanolamine 0.07%, benzyl alcohol 0.1%, phenoxyethanol 0.1%, and the balance of purified water; (4) The specific preparation process is as follows: According to the percentage in step (3), the water is uniformly stirred with the carbomer and the gelatin to obtain a mixture; then the oligoisomaltose, the glycerol, the triethanolamine, the benzyl alcohol, and the phenoxyethanol are sequentially added to the mixture for sufficient dissolution, and then the uniform oligoisomaltose gel system is obtained.

[0027] Comparative Example 7 A preparation method of an oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation, the steps are as follows: (1) The carbomer 941 and the carbomer 940 are mixed according to a mass ratio of 1:1 to obtain a carbomer for standby use; (2) The oligoisomaltose with a mass concentration of ≥50% of isomaltulose and panose is selected for standby use. (3) The oligoisomaltose gel for preventing and treating gynecological reproductive tract inflammation comprises, by mass percentage, oligoisomaltose 1%, carbomer 0.4%, gelatin 0.1%, glycerol 2%, triethanolamine 0.07%, benzyl alcohol 0.1%, phenoxyethanol 0.1%, and the balance of purified water; (4) The specific preparation process is as follows: According to the percentage in step (3), the water is stirred with the carbomer and gelatin to obtain a mixture; then the isomalt, glycerol, triethanolamine, benzyl alcohol, phenoxyethanol are sequentially added to the mixture for sufficient dissolution, and then the uniform isomalt gel system is obtained.

[0028] Comparative Example 8 A preparation method of an isomalt gel for preventing and treating inflammation of the gynecological reproductive tract, the steps are: (1) mixing carbomer 941 and carbomer 940 according to a mass ratio of 1:1 to obtain a carbomer for standby; (2) selecting isomalt and panose with a mass concentration of ≥50% for standby; (3) the isomalt gel for preventing and treating inflammation of the gynecological reproductive tract, in terms of mass percentage, comprises: isomalt 1%, carbomer 0.4%, gelatin 0.1%, glycerol 3%, triethanolamine 0.07%, benzyl alcohol 0.1%, phenoxyethanol 0.1%, and the balance of purified water; (4) the specific preparation process is: According to the percentage in step (3), the water is stirred with the carbomer and gelatin to obtain a mixture; then the isomalt, glycerol, triethanolamine, benzyl alcohol, phenoxyethanol are sequentially added to the mixture for sufficient dissolution, and then the uniform isomalt gel system is obtained.

[0029] Comparative Example 9 A preparation method of an isomalt gel for preventing and treating inflammation of the gynecological reproductive tract, the steps are: (1) only selecting carbomer 941 for standby; (2) selecting isomalt and panose with a mass concentration of ≥50% for standby; (3) the isomalt gel for preventing and treating inflammation of the gynecological reproductive tract, in terms of mass percentage, comprises: isomalt 1%, carbomer 0.4%, gelatin 0.1%, glycerol 3%, triethanolamine 0.07%, benzyl alcohol 0.1%, phenoxyethanol 0.1%, and the balance of purified water; (4) the specific preparation process is: According to the percentage in step (3), the water is stirred with the carbomer and gelatin to obtain a mixture; then the isomalt, glycerol, triethanolamine, benzyl alcohol, phenoxyethanol are sequentially added to the mixture for sufficient dissolution, and then the uniform isomalt gel system is obtained.

[0030] Application Example 1 The Candida albicans and Escherichia coli are diluted to 10 5The cfu / mL of the E. coli and the cfu / mL of the Candida albicans were mixed in a volume ratio of 1:1 to obtain a mixed bacteria solution for standby; 1 mL of the mixed bacteria solution was uniformly coated on a culture dish containing a culture medium, and an Oxford cup was vertically placed on the surface of the culture medium, and the prepared oligoisomaltose gel in Examples 1-3 and Comparative Examples 1-9 was added into the Oxford cup, and sterile water was used as a blank control in the same culture dish; each Oxford cup was one treatment, and each treatment was repeated three times, and the culture dishes were cultured in the same environment, and the average diameter of the inhibition zone was counted after 20 hours of culture, and the results are shown in Table 1.

[0031] Table 1: Inhibition effect of different treatments

[0032] As can be seen from the data in Table 1, the oligoisomaltose gel prepared by the technical scheme of the present application has better inhibition effect.

[0033] Application Example 2 (1) 10 8 cfu / mL of the E. coli and 10 8 cfu / mL of the Candida albicans were mixed in a volume ratio of 1:1 to obtain a mixed bacteria solution for standby; (2) 24 experimental rats with basically the same growth potential were injected with 0.1 mL of the mixed bacteria solution in step (1) into the vagina of each rat once a day, and after one week of continuous injection, the rat vulva was red and swollen and had secretions, indicating that the modeling was successful; (3) After the modeling was successful, the oligoisomaltose gels prepared in Examples 1-3 were used as experimental groups, and normal saline was used as a control group, and each experimental group and treatment group had 6 experimental rats; the oligoisomaltose gel (or normal saline) was injected into the vagina of the successfully modeled rats, and the drug was administered once a day, and after one week of continuous administration, the rat vulva was observed after 3 days of drug withdrawal, and the secretions were detected to check the cure rate, and the results are shown in Table 2.

[0034] Table 2: Rat vulva state and cure rate in different treatments

[0035] As can be seen from the data in Table 2, compared with the control group, the oligoisomaltose gel provided by the present application is beneficial to reducing the rat vaginal verification, and the cure rate can reach 100% after 3 days of administration.

[0036] As can be seen from the above, in the oligoisomaltose gel provided by the present application, the oligoisomaltose is specifically combined with other components, which not only can inhibit bacteria but also can promote the release of the function of healthy flora in the vagina, has strong practicability, can not only reduce the occurrence of gynecological reproductive tract inflammation, but also can quickly relieve symptoms such as redness, itching and pain.

[0037] Although the above embodiments have been described in detail, they are only some embodiments of the present application, not all embodiments. Other embodiments can be obtained on the basis of the above embodiments without creativity, which are within the protection scope of the present application.

Claims

1. An oligoisomaltose gel for use in the prevention and treatment of inflammation of the gynecological reproductive tract, characterized in that, By mass percentage, comprising: oligoisomaltose 4%-6%, carbomer 0.3%-0.5%, gelatin 0.1-0.3%, glycerol 1.5%-2.5%, triethanolamine 0.05%-0.08%, benzyl alcohol 0.1%-0.3%, phenoxyethanol 0.1%-0.5% and the balance of water.

2. The oligomerized isomalt gel according to claim 1, characterized in that, The mass concentration of isomaltulose and panose in the oligoisomaltose is ≥50%.

3. The oligomerized isomalt gel according to claim 1, characterized in that, The carbomer comprises carbomer 941 and carbomer 940.

4. The oligomerized isomalt gel according to claim 3, characterized in that, The mass ratio of the carbomer 941 and the carbomer 940 is 1:1-1.

5.

5. The oligomerized isomalt gel of claim 1, wherein The water comprises purified water.

6. The method of claim 1 5. The method of preparing the oligoisomalto- gel according to any one of claims 1 to 4, characterized in that, Comprising the following steps: After stirring the water, the carbomer and the gelatin uniformly, a mixture is obtained; The oligoisomaltose, the glycerol, the triethanolamine, the benzyl alcohol and the phenoxyethanol are sequentially added into the mixture to obtain an oligoisomaltose gel.

7. The oligomer of any one of claims 1 5 Use of the oligomer isomaltulose gel according to any one of claims 1 to 4 for the preparation of a product for the prevention and / or treatment of inflammation of the gynaecological reproductive tract.

8. Use according to claim 7, characterized in that, The performance of the gynecological genital tract inflammation comprises one or more of redness, itching and pain.

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