Composition for reducing age of intestinal tract, oral composition formed by composition and application of oral composition

Abstract

The invention provides a composition for reducing the age of the intestinal tract, an oral composition formed by the composition and application, the composition for reducing the age of the intestinal tract comprises probiotics and prebiotics, and the probiotics are bifidobacteria; the prebiotics comprise two or more than two of polydextrose, isomaltooligosacharide and galactooligosaccharide. The composition can effectively influence and change the composition of intestinal bacteria of a body, promote the abundance of core characteristic bacteria representing young intestinal tracts to be increased and inhibit the growth of bacteria representing aging intestinal tracts, and people tests prove that the composition can effectively change the intestinal flora structure tending to be aged, reduce the age of the intestinal tracts and improve the immunity of the intestinal tracts. The intestinal tract becomes young.

Description

Technical Field

[0001] This invention relates to the technical field of intestinal age adjustment, and more specifically to a composition for reducing intestinal age, an oral composition thereof, and its application. Background Technology

[0002] The gut microbiota changes at different ages, and these changes are related to human health and lifespan. When the proportion of harmful bacteria in the gut microbiota increases and the proportion of beneficial bacteria decreases, it accelerates intestinal aging and can lead to various health problems. Bifidobacteria are very important beneficial bacteria in the gut, playing a variety of important physiological roles in human health, including biological barrier function, nutritional function, anti-tumor function, immune enhancement, improving gastrointestinal function, and anti-aging. Studies have found that the proportion of bifidobacteria in the gut of long-lived elderly people, generally healthy elderly people, and sick elderly people varies greatly. The proportion of bifidobacteria in long-lived elderly people is higher than that in generally healthy elderly people, while the proportion of bifidobacteria in sick elderly people is almost zero.

[0003] Prebiotics such as galactooligosaccharides and fructooligosaccharides, also known as bifidus factors, can promote the growth and reproduction of bifidobacteria in the gut. Although prebiotics can serve as food for probiotics and be utilized by beneficial bacteria in the gut, different probiotics exhibit significant differences in their affinity for prebiotics. Studies have shown that when fructooligosaccharides are used as the sole carbon source, *Lactobacillus salivarius* W57 shows poor growth, but fructooligosaccharides can significantly promote the growth and proliferation of *Lactobacillus paracasei* W20. Specific gut microbiota selectively metabolize prebiotics. Therefore, specific combinations and ratios of prebiotics, as well as specific bond types, chain lengths, and degrees of polymerization, can be effectively utilized by specific gut microbiota. Bifidobacteria (a specific gut microbiota) have a higher utilization rate of prebiotics, thus better promoting their proliferation.

[0004] Existing technology CN105267545A discloses a traditional Chinese medicine for treating kidney yang deficiency and having anti-aging effects, and its preparation method; this patent discloses a traditional Chinese medicine formula for treating kidney yang deficiency and having anti-aging effects, characterized by being composed of the following ingredients in parts by weight: Rehmannia glutinosa 5-50, Cornus officinalis 5-50, Eucommia ulmoides 5-50, Dioscorea opposita 5-50, Atractylodes macrocephala 5-50, Psoralea corylifolia 5-45, Paeonia lactiflora 5-55, Angelica sinensis 5-60, Dioscorea hypoglauca 5-50, Plantago asiatica 5-50, Agrimonia pilosa 5-100, Morinda officinalis 5-50, Drynaria fortunei 5-46, and Euryale ferox 3-50. The main component of Rehmannia glutinosa is stachyose, which can increase the number of Bifidobacteria in the human gut by 40-100 times. An increased proportion of Bifidobacteria in the gut has a definite anti-aging effect. This patent merely infers that the traditional Chinese medicine formula has an anti-aging effect based on the fact that stachyose can increase the number of bifidus cells in the human intestine. The gut is the largest micro-ecosystem in the human body, home to over 400 types of bacteria. It absorbs and eliminates 100% of the body's nutrients and 90% of its toxins. The gut is also the largest immune organ, controlling 70% of the body's immune function. The gut is closely related to the body's life cycle. The composition of the gut microbiota changes continuously with age. In infancy, the gut is full of beneficial bacteria such as Bifidobacteria. After weaning and starting solids, the gut microbiota gradually changes, with the proportion of beneficial bacteria gradually decreasing. By adulthood, the proportion of beneficial bacteria further decreases, from 40% to about 10%. After age 60, the proportion of beneficial bacteria is only 1-5%, while harmful bacteria such as Clostridium perfringens and Escherichia coli increase significantly, leading to a marked increase in symptoms such as constipation, foul-smelling stools, and bloating. The balance of various gut microbiota is used to assess the aging state of the gut and the incidence of diseases, a concept known as "gut age."

[0005] Probiotics and prebiotics, as microecological preparations, can regulate the gut microbiota, inhibit harmful bacteria, and promote the growth of beneficial bacteria, thereby improving gut health. Most commercially available probiotic and prebiotic products for gastrointestinal health are evaluated based on their ability to regulate gut microbiota and intestinal function; however, assessments of their effectiveness in regulating the core gut microbiota and reducing gut age are rarely found. CN105267545A and CN110169570A, mentioned above, both mention regulating gut microbiota and speculate that they can delay aging or rejuvenate the gut, but they haven't actually verified whether they can effectively rejuvenate the gut. Summary of the Invention

[0006] To address the problems and shortcomings of existing technologies, this invention provides a composition that reduces intestinal age. This composition can effectively influence and alter the composition of the gut microbiota, increasing the abundance of core characteristic bacteria representing a young gut and inhibiting the growth of bacteria representing an aging gut. Human trials have confirmed that this probiotic and prebiotic composition can effectively change the structure of an aging gut microbiota, reduce intestinal age, and make the gut younger.

[0007] According to one aspect of the present invention, a composition for reducing intestinal age is provided, comprising probiotics and prebiotics, wherein the probiotics are Bifidobacteria; and the prebiotics comprise two or more of polydextrose, isomaltooligosaccharide, and galactooligosaccharide.

[0008] The gut age mentioned in this application is determined by accurately detecting the gut microbiota of the subjects, using the random forest algorithm, and leveraging machine learning and a large population cohort to build a gut age prediction model. The gut age of the subjects is determined based on the core and characteristic microbiota features of each age group, rather than simply increasing the number of bifidus in the gut to determine whether aging can be delayed.

[0009] Preferably, the mass ratio of polydextrose:isomaltooligosaccharide:galactooligosaccharide in the prebiotic is 0~2:3~5:4~5.

[0010] When the ratio of prebiotics in this composition—polydextrose:isomaltooligosaccharide:galactooligosaccharide—is 0-2:3-5:4-5, the six probiotic strains in the composition exhibit the highest utilization rate of this prebiotic combination. Human trials have confirmed that this probiotic-prebiotic composition can reduce gut age and promote a younger gut.

[0011] Preferably, the probiotics include Bifidobacterium animalis subsp. lactis, Bifidobacterium longum subsp. longum, Bifidobacterium longum subsp. infantis, Bifidobacterium adolescentis, Bifidobacterium bifidum, and Bifidobacterium breve.

[0012] The second objective of this application is to provide an oral composition, including the composition for reducing intestinal age as described above, and also including an oral carrier, diluent, excipients and other food-acceptable materials.

[0013] Preferably, the oral composition is any one of solid beverages, tablets, jellies, gummies, capsules, and drinks.

[0014] This application also provides an application of an oral composition, wherein the oral composition described above is used in food, health food, pet food or medicine, wherein the food, health food, pet food or medicine is used to alter the composition of the gut microbiota, reduce intestinal age and restore the intestinal youthfulness.

[0015] This application has the following beneficial effects: This patented composition employs a specific prebiotic combination that is better utilized by the six Bifidobacteria species within the composition, thereby promoting their more effective proliferation. These six Bifidobacteria, through their proliferation and metabolism in the gut, effectively influence and alter the composition of the gut microbiota, increasing the abundance of core characteristic bacteria representing a youthful gut and inhibiting the growth of bacteria representing an aging gut. Human trials have confirmed that this probiotic and prebiotic composition can effectively alter the aging gut microbiota structure, reduce gut age, and rejuvenate the gut. Attached Figure Description

[0016] Figure 1 This is a comparison of the changes in intestinal age between the control group and the experimental group in the experimental case. Detailed Implementation

[0017] To enable those skilled in the art to better understand the technical solutions of this invention, the technical solutions of this invention will be clearly and completely described below with reference to the embodiments and accompanying drawings. Obviously, the described embodiments are only some embodiments of this invention, and not all embodiments. Based on the embodiments of this invention, all other embodiments obtained by those skilled in the art without creative effort should fall within the scope of protection of this invention.

[0018] This application utilizes a specific prebiotic combination that is better utilized by the six Bifidobacteria species in the composition, exhibiting the highest degradation rate and thus promoting more effective proliferation of these six Bifidobacteria. These six Bifidobacteria, through proliferation and metabolism in the gut, effectively influence and alter the composition of the gut microbiota, increasing the abundance of core characteristic bacteria representing a youthful gut and inhibiting the growth of bacteria representing an aging gut. Human trials have confirmed that this probiotic and prebiotic composition can effectively alter the aging gut microbiota structure, reduce gut age, and rejuvenate the gut.

[0019] Physiological age refers to the level of physiology and function a person reaches at a certain age, measured from a medical and biological perspective. Gut age is a predictive model based on the gut microbiota composition of healthy individuals at each age, extracted using a deep neural network model to extract characteristic bacterial genera. In humans, age-related gut microbiota dysbiosis (microbial aging) is characterized by a decrease in the number of Clostridium and Bifidobacterium, and an excess of pathogenic bacteria such as Proteobacteria and Enterobacteriaceae.

[0020] The method for detecting the prebiotic degradation rate in this application is as follows: Using formic acid:n-butanol:water in a volume ratio of 6:4:1 as the developing solvent, accurately weigh 900 mg of 3,5-dihydroxytoluene and dissolve it in 25 mL of water. Add 375 mL of anhydrous ethanol, and slowly add 50 mL of concentrated sulfuric acid as the colorimetric reagent in an ice bath. Spot 0.2 μL of the solution for thin-layer chromatography. Use the unfermented culture medium at 0 h as a negative control. If the gray value of the culture medium fermented for 48 h is lower than that of the negative control, it indicates that glycogen has been degraded.

[0021] Calculation: Degradation rate = (average gray value of a sample at 0 hours - average gray value of a sample at 24 hours) / average gray value at 0 hours.

[0022] Specifically, six types of probiotics were inoculated into commercially available PYG medium and cultured in an anaerobic workstation for 48-72 hours. After activation, 200 μL of bacterial suspension was spread onto solidified PYG medium plates and cultured for 48-72 hours. Single colonies were then transferred to new plates using a sterile inoculation loop and streaked to obtain single colonies. A second streaking was then performed for purification. Finally, single colonies were picked and transferred to PYG liquid medium for enrichment culture. After the culture was completed, the bacterial suspension was collected for species identification and used in subsequent experiments.

[0023] Probiotics: After culturing six types of Bifidobacteria to the logarithmic phase, they were then subjected to a 10⁻⁶ ppm fermentation. 5 The concentration (inoculation ratio of 1:1:1:1:1:1) was inoculated into CDM fermentation vials and incubated at 37°C for 48 hours.

[0024] Prebiotics: The concentration is 1%, or 10g / L. The prebiotics here include galactooligosaccharides (GOS), isomaltooligosaccharides (IMO), and polydextrose (PDX), and are prepared according to the proportions in Table 1.

[0025] Methods for predicting gut age include: S1: The prediction model was built based on the collection of more than 300,000 population sample data. A gut microbiota-based gut age prediction model was constructed using deep neural networks and gut microbiota mapping methods.

[0026] S2: The model employs the Random Forest algorithm, formatting the data into a sparse classification table and inputting it into Random Forest regression. RF regression and a tuned Convolutional Neural Network (CNN) are used for classification to quantitatively assess the microbiome age of each patient. RF regression is used to regress the data against the chronological age. To obtain the best variance explanation associated with chronological age, the RF regression parameters are optimized as follows: R package "RF", ntree 500, using the default mtry. After 100 iterations, the algorithm outputs a report of important bacterial genera sorted by feature importance, and selects 31 genera from these. Based on all control patients included in the model, a gut microbiota development profile is plotted for each group. The model explains 47% of the variance.

[0027] S3: This gut age prediction model uses an abundance sequence cohort of microbial taxa at the OUT level from 300,000 human samples aged 1 week to 110 years as the training set to form an OTU abundance overview map. Genera are located from high to low abundance based on their abundance and correlation with specific paired genera, and training is performed based on the physiological age of the samples. 5000 simulations were conducted to confirm the results, constructing a gut microbiota-based gut age prediction model that can effectively assess the gut age of individuals aged 0–110 years.

[0028] S4: Collect fecal samples from the subjects, place sufficient fresh feces into a storage box, and allow it to fully dissolve in the protective solution within 3 minutes after defecation. Within 3 days, send the storage box containing the dissolved feces to a third-party testing agency for fecal microbiota testing.

[0029] S5: Extract total genomic DNA from microorganisms using the GHFDE100 DNA Extraction Kit, following the manufacturer's instructions. Perform quantitative and qualitative analysis of the extracted DNA using an Illumina NovaSeq6000.

[0030] S6: A random forest regression model was used to perform regression analysis on the relative abundance of the gut microbiota and the actual age of the subjects to predict the top-ranking age-discriminating marker species. Based on the composition of these marker species, the gut microbiota age index for each sample subgroup at each time point was calculated. A low gut microbiota age index indicates that the fecal microbiota is "young," and the gut age is calculated based on the age index.

[0031] Example 1 This embodiment provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include Bifidobacterium animalis subsp. lactis, Bifidobacterium longum subsp. longum, Bifidobacterium longum subsp. infantis, Bifidobacterium adolescentis, Bifidobacterium bifidum, and Bifidobacterium breve; and the concentration ratio of Bifidobacterium animalis subsp. lactis, Bifidobacterium longum subsp. longum, Bifidobacterium longum subsp. infantis, Bifidobacterium adolescentis, Bifidobacterium bifidum, and Bifidobacterium breve in the probiotics is 1:1:1:1:1:1.

[0032] The prebiotics include polydextrose, isomaltooligosaccharide, and galactooligosaccharide; the mass ratio of polydextrose to isomaltooligosaccharide to galactooligosaccharide in the prebiotics is 0:5:5.

[0033] Example 2 This embodiment provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*; and the concentration ratio of *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve* in the probiotics is 1:1:1:1:1:1:1 The prebiotics include polydextrose, isomaltooligosaccharide, and galactooligosaccharide; the mass ratio of polydextrose to isomaltooligosaccharide to galactooligosaccharide in the prebiotics is 2:3:5.

[0034] Example 3 This embodiment provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*; and the concentration ratio of *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve* in the probiotics is 1:1:1:1:1:1:1 The prebiotics include polydextrose, isomaltooligosaccharide, and galactooligosaccharide; the mass ratio of polydextrose:isomaltooligosaccharide:galactooligosaccharide in the prebiotics is 1.5:4.5:4.

[0035] Example 4 This embodiment provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*; and the concentration ratio of *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve* in the probiotics is 1:1:1:1:1:1:1 The prebiotics include polydextrose, isomaltooligosaccharide, and galactooligosaccharide; the mass ratio of polydextrose to isomaltooligosaccharide to galactooligosaccharide in the prebiotics is 1:4:5.

[0036] Example 5 This embodiment provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*; and the concentration ratio of *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve* in the probiotics is 1:1:1:1:1:1:1 The prebiotics include polydextrose, isomaltooligosaccharide, and galactooligosaccharide; the mass ratio of polydextrose to isomaltooligosaccharide to galactooligosaccharide in the prebiotics is 1:5:4.

[0037] Comparative Example 1 This study provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*; and the concentration ratio of *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve* in the probiotics is 1:1:1:1:1:1; The prebiotic is galactooligosaccharide.

[0038] Comparative Example 2 This comparative example provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*; and the concentration ratio of *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve* in the probiotics is 1:1:1:1:1:1:1 The prebiotic is isomaltooligosaccharide.

[0039] Comparative Example 3 This comparative example provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*; and the concentration ratio of *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve* in the probiotics is 1:1:1:1:1:1:1 The prebiotic is polydextrose.

[0040] Comparative Example 4 This comparative example provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*; and the concentration ratio of *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve* in the probiotics is 1:1:1:1:1:1:1 The prebiotics include polydextrose and galactooligosaccharides; the mass ratio of polydextrose to galactooligosaccharides in the prebiotics is 1:1.

[0041] Comparative Example 5 This comparative example provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*; and the concentration ratio of *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve* in the probiotics is 1:1:1:1:1:1:1 The prebiotics include polydextrose and isomaltooligosaccharide; the mass ratio of polydextrose to isomaltooligosaccharide in the prebiotics is 1:1.

[0042] Comparative Example 6 This comparative example provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*; and the concentration ratio of *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve* in the probiotics is 1:1:1:1:1:1:1 The prebiotics include polydextrose, isomaltooligosaccharide, and galactooligosaccharide; the mass ratio of polydextrose:isomaltooligosaccharide:galactooligosaccharide in the prebiotics is 1.5:1.5:7.

[0043] Comparative Example 7 This comparative example provides a composition for reducing gut age, comprising probiotics and prebiotics; The probiotics include *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*; and the concentration ratio of *Bifidobacterium animalis* subsp. *lactamase*, *Bifidobacterium longum* subsp. *longum*, *Bifidobacterium longum* subsp. *infant*, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve* in the probiotics is 1:1:1:1:1:1:1 The prebiotics include polydextrose, isomaltooligosaccharide, and galactooligosaccharide; the mass ratio of polydextrose:isomaltooligosaccharide:galactooligosaccharide in the prebiotics is 0.7:1.5:1.5.

[0044] Table 1. Prebiotic ratios in Examples 1-5 and Comparative Examples 1-7

[0045] A higher degradation rate indicates better metabolic utilization of carbon sources (prebiotics) by microorganisms (probiotics). Table 1 shows that when the ratio of polydextrose:isomaltooligosaccharide:galactooligosaccharide is 0~2:3~5:4~5, the utilization rate of this prebiotic combination by the six bifidobacteria is the highest, reaching over 58%; this composition ratio can better promote the proliferation of these six bifidobacteria.

[0046] Population testing experiment example The prebiotic compositions from Examples 1-5 were combined with six types of bifidobacteria to formulate products, which were then further tested in populations. The results showed that, compared to placebo, all of these combinations effectively reduced the gut age of the subjects. The average effect of these combinations on gut age reduction was calculated as follows: Figure 1 The probiotic and prebiotic combination can significantly reduce gut age (p<0.01), making the gut younger.

[0047] The specific testing process is as follows: Target population: Healthy subjects aged 25-65 Experimental methods and design: randomized, single-blind, parallel-controlled. Grouping: A placebo control group (n=24) and a probiotic group (n=25). The placebo group received maltodextrin, while the probiotic group received a probiotic-prebiotic combination. The probiotics included *Bifidobacterium animalis* subsp. lactis, *Bifidobacterium longum* subsp. longum, *Bifidobacterium longum* subsp. infantis, *Bifidobacterium adolescentis*, *Bifidobacterium bifidum*, and *Bifidobacterium breve*, in a concentration ratio of 1:1:1:1:1:1. The prebiotics included polydextrose, isomaltooligosaccharide, and galactooligosaccharide, with a polydextrose:isomaltooligosaccharide:galactooligosaccharide ratio of 0~2:3~5:4~5. In this experimental example, the probiotic group used five of each of the combinations from Examples 1-5, forming 25 probiotic groups. The intestinal age difference values ​​obtained before and after administration of the probiotics were averaged to obtain the intestinal age difference value for each probiotic group.

[0048] Testing period: 60 days Testing indicator: Gut age. Specifically, at baseline (before testing) and 60 days after taking the sample, a small amount of fresh stool was collected using a sampling swab. The discolored swab tip was immersed in the sampling solution in the sampling tube, and the swab was rotated and stirred for 30 seconds. The swab was then removed and discarded. The sampling tube cap was tightened, and the tube was shaken from side to side for about 10 seconds to complete the sampling. The stool samples were analyzed for gut microbiota using an Illumina NovaSeq 6000.

[0049] The intestinal age was detected using the method described above, and the difference in intestinal age before and after the test was as follows: Figure 1 As shown; where Figure 1 The data for the probiotic group is the average of the effects of Examples 1-5 on reducing intestinal age; this shows that the probiotic and prebiotic composition in this application can significantly reduce intestinal age (p<0.01), making the gut younger.

[0050] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for reducing intestinal age, characterized in that, It includes probiotics and prebiotics, wherein the probiotics are Bifidobacteria; and the prebiotics include two or more of polydextrose, isomaltooligosaccharide, and galactooligosaccharide.

2. The composition for reducing intestinal age according to claim 1, characterized in that, The mass ratio of polydextrose, isomaltooligosaccharide, and galactooligosaccharide in the prebiotic is 0~2:3~5:4~5.

3. The composition for reducing intestinal age according to claim 1, characterized in that, The probiotics include Bifidobacterium animalis subsp. lactis, Bifidobacterium longum subsp. longum, Bifidobacterium longum subsp. infantis, Bifidobacterium adolescentis, Bifidobacterium bifidum, and Bifidobacterium breve.

4. An oral composition, characterized in that, The composition comprising any one of claims 1-3 for reducing intestinal age also includes an oral carrier, a diluent, an excipient, and other food-acceptable materials.

5. The oral composition according to claim 4, characterized in that, The oral composition is any one of solid beverages, tablets, jellies, gummies, capsules, and drinks.

6. The use of an oral composition, characterized in that, The oral composition of claim 4 is used in food, health food, pet food or medicine, wherein the food, health food, pet food or medicine is used to change the composition of the intestinal flora, reduce intestinal age and restore the intestinal youth.

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