Natural polysaccharide composition and application thereof in preparation of product for relieving constipation by promoting peristalsis

Abstract

The invention relates to the technical field of biological medicines, in particular to a natural polysaccharide composition and application thereof in preparation of a product for relieving constipation by promoting peristalsis. The natural polysaccharide composition comprises mint polysaccharide and astragalus polysaccharide; wherein the mass ratio of the mint polysaccharide to the astragalus polysaccharide is 1: (1-30). Researches show that the natural polysaccharide composition obtained by combining the mint polysaccharide and the astragalus polysaccharide has the effect of promoting motilin secretion remarkably higher than that of single mint polysaccharide or astragalus polysaccharide; the synergistic interaction effect is achieved. The natural polysaccharide composition disclosed by the invention has the effect of promoting motilin secretion, so that the natural polysaccharide composition can further play a role in promoting peristalsis and further play a role in relieving constipation; the important application value is realized.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological medicine, in particular to a natural polysaccharide composition and application thereof in preparation of products for relieving constipation by promoting intestinal peristalsis. BACKGROUND

[0002] Constipation is mainly manifested as reduced defecation frequency, dry and hard stool and defecation difficulty. In modern life, constipation has become a common digestive system problem, which has a serious impact on people's quality of life. Studies have shown that slow intestinal peristalsis is an important inducement of constipation. Under normal circumstances, the intestinal tract pushes food residues to the anus through regular peristalsis, if the intestinal peristalsis slows down, the food residues stay in the intestinal tract for a long time, and water is excessively absorbed, thereby causing constipation. In order to relieve constipation, the current market treatment methods include the use of chemical drugs, the change of eating habits and the increase of physical activity, etc. However, chemical drugs have a series of side effects, such as dependence, electrolyte disorder, etc., and long-term use has potential risks to human health. In contrast, natural active substances are attracting more and more attention in the field of constipation relief and treatment due to their natural, safe and small side effects.

[0003] Motilin is a kind of polypeptide gastrointestinal hormone secreted by digestive tract endocrine cells; it is one of the key hormones for regulating intestinal peristalsis, and its secretion rhythm and level directly affect the movement ability and emptying efficiency of the intestinal tract. When developing functional products or drugs for promoting intestinal peristalsis, enhancing the secretion or activity of motilin is an important approach.

[0004] Radix Astragali is a commonly used traditional Chinese medicine and food with medicinal value, and its main component polysaccharide has various pharmacological activities. Studies have shown that Radix Astragali active substances can enhance the body's immunity. Mint is a common natural plant and food with medicinal value, which is rich in various active ingredients and has various pharmacological activities. Among them, mint extract has certain anti-inflammatory effect, can reduce intestinal inflammation and improve intestinal dysfunction, etc.

[0005] Studies have reported that Radix Astragali and mint are rich in sugar active ingredients and have various pharmacological activities, but there are few studies and reports on the enrichment and separation of Radix Astragali and mint and their combination for promoting the secretion of motilin, treating constipation, especially the research and application of exerting the respective advantages of polysaccharides in Radix Astragali and mint and producing synergistic effect. SUMMARY

[0006] In order to overcome at least one of the technical problems in the prior art, the present application provides a natural polysaccharide composition and application thereof in preparation of products for relieving constipation by promoting intestinal peristalsis.

[0007] The technical scheme of the present application is as follows: The application provides a natural polysaccharide composition which is composed of mentha polysaccharide and astragalus polysaccharide.

[0008] The application provides a natural polysaccharide composition which is composed of mentha polysaccharide and astragalus polysaccharide.

[0009] Preferably, the mass ratio of the mentha polysaccharide to the astragalus polysaccharide is 1: (1-10).

[0010] Most preferably, the mass ratio of the mentha polysaccharide to the astragalus polysaccharide is 1: 3.35.

[0011] Preferably, the mentha polysaccharide and the astragalus polysaccharide are prepared by a water extraction and alcohol precipitation method.

[0012] Preferably, the mentha polysaccharide or the astragalus polysaccharide is prepared by the following method: (1) fresh mentha or astragalus is extracted with water, and the filtrate is obtained after filtration; (2) the filtrate is concentrated to obtain a concentrated solution, and ethanol is added to the concentrated solution to obtain a mixed solution; (3) the mixed solution is placed at 1-5 ℃ for 8-16 h, and the precipitate is obtained after filtration, and the mentha polysaccharide or the astragalus polysaccharide is obtained.

[0013] Preferably, the use amount ratio of the fresh mentha or astragalus to water is 1 kg: 8-15 L.

[0014] Most preferably, the use amount ratio of the fresh mentha or astragalus to water is 1 kg: 12 L.

[0015] Preferably, the extraction in step (1) is heating reflux extraction, the extraction temperature is 80-100 ℃, and the extraction time is 0.5-3 h.

[0016] Most preferably, the extraction in step (1) is heating reflux extraction, the extraction temperature is 90 ℃, and the extraction time is 1.5 h.

[0017] Preferably, the filtrate is concentrated to 1 / 4-1 / 8 of the volume of the filtrate in step (2).

[0018] Most preferably, the filtrate is concentrated to 1 / 5 of the volume of the filtrate in step (2).

[0019] Preferably, in step (2), the ethanol in the mixed solution has a volume concentration of 70-85%.

[0020] Preferably, in step (2), the concentrated solution is added with ethanol to obtain a mixed solution, so that the volume concentration of ethanol in the mixed solution is 80%.

[0021] Preferably, in step (3), the mixed solution is allowed to stand at 4℃ for 8h.

[0022] The application further provides a preparation method of the natural polysaccharide composition, comprising the following steps: uniformly mixing the mint polysaccharide and the astragalus polysaccharide to obtain a mixture, and taking the mixture to obtain the natural polysaccharide composition.

[0023] Preferably, the preparation method of the natural polysaccharide composition further comprises the following steps: dissolving the mixture in water, then performing ultrafiltration on the mixture with an ultrafiltration membrane with a molecular weight of 3 kDa-4 kDa to obtain an ultrafiltration solution A and a concentrated solution A; taking the ultrafiltration solution A and performing ultrafiltration on the ultrafiltration solution A with an ultrafiltration membrane with a molecular weight of 1.5 kDa-2 kDa to obtain an ultrafiltration solution B and a concentrated solution B; and taking the concentrated solution B and concentrating and drying the concentrated solution B to obtain the natural polysaccharide composition.

[0024] Most preferably, the preparation method of the natural polysaccharide composition further comprises the following steps: dissolving the mixture in water, then performing ultrafiltration on the mixture with an ultrafiltration membrane with a molecular weight of 3.5 kDa to obtain an ultrafiltration solution A and a concentrated solution A; taking the ultrafiltration solution A and performing ultrafiltration on the ultrafiltration solution A with an ultrafiltration membrane with a molecular weight of 1.8 kDa to obtain an ultrafiltration solution B and a concentrated solution B; and taking the concentrated solution B and concentrating and drying the concentrated solution B to obtain the natural polysaccharide composition.

[0025] Preferably, the mass ratio of the mixture to water is 1:5-15.

[0026] Most preferably, the mass ratio of the mixture to water is 1:10.

[0027] The inventors have found in further research that the natural polysaccharide composition obtained by the ultrafiltration method of the application has a further greatly improved effect of promoting the secretion of motilin compared to the natural polysaccharide composition obtained without the ultrafiltration method of the application.

[0028] The inventors have also found in research that the ultrafiltration conditions are very critical; only the natural polysaccharide composition obtained by the ultrafiltration method of the application can have a further greatly improved effect of promoting the secretion of motilin; however, the natural polysaccharide composition obtained by other ultrafiltration methods cannot have a further greatly improved effect of promoting the secretion of motilin.

[0029] The application further provides a use of the natural polysaccharide composition in the preparation of a product with an effect of promoting the secretion of motilin.

[0030] The present invention also provides the application of the above-mentioned natural polysaccharide composition in the preparation of products that promote intestinal peristalsis.

[0031] The present invention also provides the application of the above-mentioned natural polysaccharide composition in the preparation of products that relieve constipation by promoting intestinal peristalsis.

[0032] Beneficial Effects: This invention provides a novel natural polysaccharide composition composed of peppermint polysaccharide and astragalus polysaccharide. Studies have shown that the natural polysaccharide composition obtained by combining peppermint and astragalus polysaccharide has a significantly higher motilin-promoting effect than peppermint or astragalus polysaccharide alone, exhibiting a synergistic effect. Because the natural polysaccharide composition of this invention promotes motilin secretion, it can further promote intestinal peristalsis, thereby relieving constipation; thus possessing significant application value. Detailed Implementation

[0033] The present invention will be further described in detail below with reference to specific embodiments, but the embodiments do not limit the present invention in any way.

[0034] Example 1: Preparation of a natural polysaccharide composition Preparation method: Peppermint polysaccharide and astragalus polysaccharide are mixed evenly to obtain a mixture, and the mixture is taken to obtain the natural polysaccharide composition; wherein, the mass ratio of peppermint polysaccharide to astragalus polysaccharide is 1:3.35.

[0035] The peppermint polysaccharide was prepared by the following method: (1) Take fresh mint, heat it with water to 90℃ for 1.5h, and filter it after extraction to obtain the filtrate; The ratio of fresh mint to water is 1kg:12L; (2) Concentrate the filtrate to 1 / 5 of its volume to obtain a concentrated solution; add ethanol to the concentrated solution to obtain a mixed solution; wherein the volume concentration of ethanol in the mixed solution is 80%; (3) The mixture was allowed to stand at 4°C for 8 hours. After standing, the precipitate was obtained by filtration. The precipitate was then used to obtain the peppermint polysaccharide.

[0036] The aforementioned Astragalus polysaccharide was prepared by the following method: (1) Take fresh Astragalus membranaceus, heat it with water to 90℃ for 1.5h, and filter it after extraction to obtain the filtrate; The ratio of fresh Astragalus membranaceus to water is 1kg:12L; (2) Concentrate the filtrate to 1 / 5 of its volume to obtain a concentrated solution; add ethanol to the concentrated solution to obtain a mixed solution; wherein the volume concentration of ethanol in the mixed solution is 80%; (3) The mixture was allowed to stand at 4°C for 8 hours. After standing, the precipitate was obtained by filtration. The precipitate was then used to obtain the Astragalus polysaccharide.

[0037] Preparation of natural polysaccharide composition Preparation method: (1) The mint polysaccharide and the astragalus polysaccharide are mixed uniformly to obtain a mixture; wherein the mass ratio of the mint polysaccharide to the astragalus polysaccharide is 1:3.35.

[0038] (2) The mixture is dissolved in 10 times mass of water, then ultrafiltration is performed with an ultrafiltration membrane with a molecular weight of 3.5 kDa to obtain an ultrafiltration liquid A and a concentrated liquid A; the ultrafiltration liquid A is taken and ultrafiltration is performed with an ultrafiltration membrane with a molecular weight of 1.8 kDa to obtain an ultrafiltration liquid B and a concentrated liquid B; the concentrated liquid B is concentrated and dried to obtain the natural polysaccharide composition.

[0039] The preparation method of the mint polysaccharide and the astragalus polysaccharide is the same as that in Embodiment 1.

[0040] Preparation of natural polysaccharide composition Preparation method: (1) The mint polysaccharide and the astragalus polysaccharide are mixed uniformly to obtain a mixture; wherein the mass ratio of the mint polysaccharide to the astragalus polysaccharide is 1:3.35.

[0041] (2) The mixture is dissolved in 10 times mass of water, then ultrafiltration is performed with an ultrafiltration membrane with a molecular weight of 3.5 kDa to obtain an ultrafiltration liquid A and a concentrated liquid A; the ultrafiltration liquid A is taken to obtain the natural polysaccharide composition.

[0042] The preparation method of the mint polysaccharide and the astragalus polysaccharide is the same as that in Embodiment 1.

[0043] Preparation of natural polysaccharide composition Preparation method: (1) The mint polysaccharide and the astragalus polysaccharide are mixed uniformly to obtain a mixture; wherein the mass ratio of the mint polysaccharide to the astragalus polysaccharide is 1:3.35.

[0044] (2) The mixture is dissolved in 10 times mass of water, then ultrafiltration is performed with an ultrafiltration membrane with a molecular weight of 3.5 kDa to obtain an ultrafiltration liquid A and a concentrated liquid A; the ultrafiltration liquid A is taken and ultrafiltration is performed with an ultrafiltration membrane with a molecular weight of 1.8 kDa to obtain an ultrafiltration liquid B and a concentrated liquid B; the concentrated liquid B is concentrated and dried to obtain the natural polysaccharide composition.

[0045] The preparation method of the mint polysaccharide and the astragalus polysaccharide is the same as that in Embodiment 1.

[0046] Preparation of natural polysaccharide composition Preparation method: (1) The mint polysaccharide and the astragalus polysaccharide are mixed uniformly to obtain a mixture; wherein the mass ratio of the mint polysaccharide to the astragalus polysaccharide is 1:3.35.

[0047] (2) The mixture is dissolved in 10 times the mass of water, then ultrafiltration is performed using an ultrafiltration membrane with a molecular weight of 5 kDa to obtain an ultrafiltrate A and a concentrated solution A; the ultrafiltrate A is taken and ultrafiltration is performed using an ultrafiltration membrane with a molecular weight of 3 kDa to obtain an ultrafiltrate B and a concentrated solution B; the concentrated solution B is concentrated and dried to obtain the natural polysaccharide composition.

[0048] The preparation method of the peppermint polysaccharide and the astragalus polysaccharide is the same as in Example 1.

[0049] Preparation of a natural polysaccharide composition in Comparative Example 4 Preparation method: (1) The peppermint polysaccharide and the astragalus polysaccharide are uniformly mixed to obtain a mixture; the mass ratio of the peppermint polysaccharide to the astragalus polysaccharide is 1:3.35.

[0050] (2) The mixture is dissolved in 10 times the mass of water, then ultrafiltration is performed using an ultrafiltration membrane with a molecular weight of 2 kDa to obtain an ultrafiltrate A and a concentrated solution A; the ultrafiltrate A is taken and ultrafiltration is performed using an ultrafiltration membrane with a molecular weight of 0.5 kDa to obtain an ultrafiltrate B and a concentrated solution B; the concentrated solution B is concentrated and dried to obtain the natural polysaccharide composition.

[0051] The preparation method of the peppermint polysaccharide and the astragalus polysaccharide is the same as in Example 1.

[0052] Evaluation of the activity of the natural polysaccharide composition in Example 4 The samples to be tested in this experimental example are the peppermint polysaccharide prepared according to the method in Example 1, the astragalus polysaccharide prepared according to the method in Example 1, the natural polysaccharide compositions prepared in Examples 1-2 and Comparative Examples 1-4.

[0053] (1) The samples to be tested are dissolved in distilled water to prepare solutions with a concentration of 1 g / mL.

[0054] (2) After 1 week of adaptive feeding, 5-week-old healthy female Kunming mice (19-22 g) are randomly divided into groups, 8 mice per group. Except for the blank control group, the rest of the mice are given loperamide by gavage at a dose of 10 mg / kg once a day for 7 consecutive days to inhibit intestinal peristalsis. From the second day after modeling, the mice in the drug intervention group are given the active substance by gavage at a dose of 0.2 mL / mouse / day once a day for 7 consecutive days, and the mice in the blank control group and the model group are given the same volume of normal saline. 24 hours after the end of administration on the 7th day, the content of motilin in the serum of the mice is detected by enzyme-linked immunosorbent assay (ELISA); the content of motilin in the serum of the blank group is taken as 100%, and the content of motilin in the serum of the other groups is taken as a relative value; the results are shown in Table 1.

[0055] Experimental grouping: blank group (without any treatment); model group (gavaged with loperamide); experimental group 1 (gavaged with loperamide and peppermint polysaccharide prepared by the method of Example 1), experimental group 2 (gavaged with loperamide and astragalus polysaccharide prepared by the method of Example 1); experimental group 3 (gavaged with loperamide and natural polysaccharide composition prepared by Example 1); experimental group 4 (gavaged with loperamide and natural polysaccharide composition prepared by Example 2); experimental group 5 (gavaged with loperamide and natural polysaccharide composition prepared by Comparative Example 1); experimental group 6 (gavaged with loperamide and natural polysaccharide composition prepared by Comparative Example 2); experimental group 7 (gavaged with loperamide and natural polysaccharide composition prepared by Comparative Example 3); experimental group 8 (gavaged with loperamide and natural polysaccharide composition prepared by Comparative Example 4).

[0056] Table 1. Evaluation results of activity experiment of the composition Test sample Motilin relative content Blank group - 100±9.63 Model group - 70.9±6.53 Experimental group 1 Natural polysaccharide composition prepared by the method of Example 1 80.4±6.52 Experimental group 2 Natural polysaccharide composition prepared by the method of Example 1 83.6±6.88 Experimental group 3 Natural polysaccharide composition prepared by the method of Example 1 94.3±8.15 Experimental group 4 Natural polysaccharide composition prepared by the method of Example 2 133.9±11.21 Experimental group 5 Natural polysaccharide composition prepared by the method of Comparative Example 1 111.2±9.97 Experimental group 6 Natural polysaccharide composition prepared by the method of Comparative Example 2 99.3±9.14 Experimental group 7 Natural polysaccharide composition prepared by the method of Comparative Example 3 102.1±9.36 Experimental group 8 Natural polysaccharide composition prepared by the method of Comparative Example 4 105.1±9.83 As can be seen from the experimental results in Table 1, the relative content of motilin in experimental group 3 is significantly higher than that in experimental groups 1 and 2; this shows that the natural polysaccharide composition obtained by combining peppermint polysaccharide and astragalus polysaccharide has a significantly higher effect of promoting secretion of motilin than single peppermint polysaccharide or astragalus polysaccharide; the combination of peppermint polysaccharide and astragalus polysaccharide can have a synergistic effect of promoting secretion of motilin.

[0057] As can be seen from the experimental results in Table 1, the relative content of motilin in experimental group 4 is significantly higher than that in experimental group 3; this shows that the natural polysaccharide composition obtained by ultrafiltration by the ultrafiltration method of the application has a further significantly improved effect of promoting secretion of motilin compared to the natural polysaccharide composition obtained without ultrafiltration by the ultrafiltration method of the application.

[0058] As can be further seen from the experimental results in Table 1, the relative content of motilin in experimental groups 5-8 is higher than that in experimental group 3, but the improvement is not large, and the improvement is much smaller than that in experimental group 4; this shows that the ultrafiltration conditions described in the application are very critical; only the natural polysaccharide composition obtained by ultrafiltration by the ultrafiltration method of the application can have a further significantly improved effect of promoting secretion of motilin; however, the natural polysaccharide composition obtained by ultrafiltration by other methods cannot have a further significantly improved effect of promoting secretion of motilin.

Claims

1. A natural polysaccharide composition, characterized in that, The natural polysaccharide composition comprises menthol polysaccharide and astragalus polysaccharide, and the mass ratio of the menthol polysaccharide to the astragalus polysaccharide is 1:(1-30).

2. The natural polysaccharide composition according to claim 1, characterized in that, The mass ratio of the menthol polysaccharide to the astragalus polysaccharide is 1:(1-10).

3. The natural polysaccharide composition according to claim 1, characterized in that, The menthol polysaccharide and the astragalus polysaccharide are prepared by water extraction and alcohol precipitation.

4. The natural polysaccharide composition according to claim 3, characterized in that, The menthol polysaccharide or the astragalus polysaccharide is prepared by the following method: (1) fresh mint or astragalus is taken, and water is used for extraction, and after the extraction is finished, the filtrate is obtained by filtration; (2) the filtrate is concentrated to obtain a concentrated solution, and ethanol is added to the concentrated solution to obtain a mixed solution; (3) the mixed solution is placed at 1-5 ℃ for 8-16 h, and after the standing is finished, the precipitate is obtained by filtration, and the precipitate is taken to obtain the menthol polysaccharide or the astragalus polysaccharide.

5. The natural polysaccharide composition according to claim 4, characterized in that, The use amount ratio of the fresh mint or the astragalus to the water is 1 kg:8-15 L.

6. The natural polysaccharide composition according to claim 5, characterized in that, In step (2), the ethanol is added to the concentrated solution to obtain the mixed solution, so that the volume concentration of the ethanol in the mixed solution is 80%.

7. A process for the preparation of the natural polysaccharide composition according to any one of claims 1 to 6, characterized in that, The natural polysaccharide composition comprises the following steps: the menthol polysaccharide and the astragalus polysaccharide are uniformly mixed to obtain a mixture, and the mixture is taken to obtain the natural polysaccharide composition.

8. The use of the natural polysaccharide composition according to any one of claims 1-6 in the preparation of a product having the effect of promoting the secretion of motilin.

9. The use of the natural polysaccharide composition according to any one of claims 1-6 in the preparation of a product having the effect of promoting intestinal peristalsis.

10. The use of the natural polysaccharide composition according to any one of claims 1-6 in the preparation of a product for relieving constipation by promoting intestinal peristalsis.