Traditional Chinese medicine composition for clearing heat and resisting viruses, traditional Chinese medicine preparation and preparation method of traditional Chinese medicine preparation

Abstract

The invention relates to the technical field of traditional Chinese medicine preparations, and particularly discloses a scourge-clearing and antiviral traditional Chinese medicine composition, a traditional Chinese medicine preparation and a preparation method thereof. The antipyretic and antiviral traditional Chinese medicine composition is prepared from the following raw material components in parts by mass: 10 to 20 parts of garlic, 15 to 25 parts of herba schizonepetae, 5 to 10 parts of herba artemisiae annuae, 5 to 10 parts of radix isatidis, 5 to 10 parts of flos lonicerae, 5 to 10 parts of folium eriobotryae, 1 to 10 parts of radix fici simplicissimae, 1 to 5 parts of radix astragali seu hedysari, 1 to 5 parts of folium perillae, 1 to 5 parts of pericarpium zanthoxyli, 1 to 5 parts of borneol and 1 to 5 parts of natural camphor. The traditional Chinese medicine preparation prepared from the traditional Chinese medicine composition has excellent swelling and pain relieving effects, the preparation method is simple and convenient, the cost is low, and the problems that in the prior art, a scourge-clearing and antiviral traditional Chinese medicine preparation is tedious in preparation process, high in cost and not remarkable in medicine effect are effectively solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine composition for clearing away epidemic febrile disease and resisting virus, a traditional Chinese medicine preparation and a preparation method thereof. BACKGROUND

[0002] In the process of poultry breeding, viral infectious diseases are a common and harmful disease, such as Newcastle disease of chickens. The body temperature of the chicken with typical Newcastle disease is high, the spirit is depressed, the appetite is decreased or disappeared; the eyes are closed, the neck is shrunk, the wings are drooped; the breathing is difficult, the mouth emits snoring sound, there is a large amount of mucus, the crop is swollen, filled with liquid and gas, and the mucus flows out of the mouth when the chicken is turned upside down; the head is twisted, the circle is turned; the yellow-greenish thin stool is pulled; through pathological dissection, the crop of the chicken is empty, filled with liquid or gas with sour smell; there are bleeding points or bleeding belts at the junction of the esophagus and the glandular stomach, the junction of the glandular stomach and the muscular stomach; the glandular stomach nipple bleeds, the muscular stomach bleeds under the cutin layer; there are purple red, jujube kernel size swollen intestinal lymph nodes on the outside of the intestinal tract, and the intestinal mucosa surface is necrotic and ulcerated when the intestinal tract is cut open; the cecal tonsil is swollen, bled and necrotic; the rectal mucosa has point or strip bleeding; the follicle is liquefied, and there is a light yellow turbid liquid in the abdominal cavity; there are bleeding points on the larynx, the trachea ring bleeds, and there is blood mucus in the trachea.

[0003] The traditional Chinese medicine preparation for clearing away epidemic febrile disease and resisting virus can prevent before the occurrence of epidemic disease, reduce the risk of chicken infection with virus; after the occurrence of epidemic disease, it can effectively relieve the symptoms, promote the recovery of the sick chicken and reduce the breeding loss. However, the existing traditional Chinese medicine preparation for clearing away epidemic febrile disease and resisting virus still has certain deficiencies in the aspects of curative effect, safety and stability. Part of the traditional Chinese medicine composition has complex formula and complicated preparation process, resulting in high cost; part of the traditional Chinese medicine composition has insufficient curative effect and cannot quickly and effectively relieve the spread of Newcastle disease of chicken group. Therefore, it is of important practical significance to develop a traditional Chinese medicine preparation with exact curative effect, simple preparation and high efficiency of clearing away epidemic febrile disease and resisting virus. SUMMARY

[0004] In view of the problems of complicated preparation process, high cost and insufficient curative effect of the traditional Chinese medicine preparation for clearing away epidemic febrile disease and resisting virus in the prior art, the present application provides a traditional Chinese medicine composition for clearing away epidemic febrile disease and resisting virus, a traditional Chinese medicine preparation and a preparation method thereof. The raw materials of the traditional Chinese medicine composition for clearing away epidemic febrile disease and resisting virus include garlic, schizonepeta, artemisia, isatis root, honeysuckle, loquat leaf, figwort root, astragalus root, perilla, zanthoxylum, borneol and natural camphor. The present application prepares a traditional Chinese medicine preparation for clearing away epidemic febrile disease and resisting virus by taking the traditional Chinese medicine composition as the main raw material. The traditional Chinese medicine preparation has excellent heat-clearing and antiviral effect, can regulate the immune function of the body, relieve the symptoms, improve the resistance of the body at the same time, and the preparation method is simple, low in cost, which makes up for the deficiencies of the prior art.

[0005] In order to achieve the above application purposes, the present application provides the following technical solutions: The first aspect of the present application provides a traditional Chinese medicine composition for clearing away epidemic diseases and resisting viruses, comprising the following raw material components in mass fraction: garlic 10-20 parts, schizonepeta 15-25 parts, artemisia 5-10 parts, radix isatidis 5-10 parts, honeysuckle 5-10 parts, loquat leaf 5-10 parts, ficus hirta 1-10 parts, astragalus 1-5 parts, perilla 1-5 parts, zanthoxylum 1-5 parts, borneol 1-5 parts and natural camphor 1-3 parts.

[0006] Compared with the prior art, the present application prepares a traditional Chinese medicine composition for clearing away epidemic diseases and resisting viruses by taking garlic, schizonepeta, artemisia, radix isatidis, honeysuckle, loquat leaf, ficus hirta, astragalus, perilla, zanthoxylum, borneol and natural camphor as raw materials. Among them, garlic, schizonepeta, artemisia and radix isatidis are monarch drugs; allicin contained in garlic can destroy the sulfhydryl in the sulfur amino acids necessary for Newcastle disease virus and bacteria, causing metabolic disorders, so that they cannot reproduce and grow, and have excellent antibacterial and anti-inflammatory effects. Moreover, garlic can stimulate the immune system of the chicken body, enhance the activity of immune cells such as macrophages and lymphocytes, and promote the secretion of immune factors such as interleukins and interferons, which can recognize and remove viruses, improve the resistance of the chicken body, and help the chicken better resist Newcastle disease virus. Schizonepeta has the effect of relieving fever and inducing perspiration, and chickens usually have a fever after being infected with Newcastle disease, and schizonepeta helps to reduce the body temperature of sick chickens and relieve the discomfort caused by fever. Artemisia can promote the humoral immunity of the chicken body and enhance the immune defense ability of the body. Moreover, some chemical components contained in artemisia, such as flavonoids and terpenes, can effectively inhibit the reproduction and growth of Newcastle disease virus, these components can interfere with the replication process of the virus and prevent the virus from reproducing in large quantities in the chicken body, thereby reducing the damage of the virus to the chicken body. Radix isatidis is cold in nature and bitter in taste, and has the effects of clearing heat and detoxifying, cooling blood and benefiting the throat, and contains various antiviral effective components such as indigotin.

[0007] The invention takes honeysuckle, loquat leaves, figwort and astragalus as ministerial medicines; chlorogenic acid and luteolin in honeysuckle can prevent viruses from entering host cells by inhibiting the adsorption, invasion and hatching of viruses, thereby reducing the reproduction of viruses in the body; luteolin can interfere with the synthesis of viral nucleic acid to some extent, affect the normal replication of viruses and reduce the infection ability of viruses. The effective components in loquat leaves have excellent antibacterial and anti-inflammatory effects, can effectively reduce inflammatory reactions, relieve symptoms such as fever and cough in chickens, help maintain the health of respiratory tract and digestive tract and other parts of chickens, reduce the probability of complications and promote the recovery of chickens. Figwort has the effects of invigorating the spleen and lung, tonifying qi and consolidating the exterior, can promote gastrointestinal peristalsis and improve the digestive function of chickens. After being infected with Newcastle disease, chickens often have symptoms such as loss of appetite and indigestion, which affect the absorption and utilization of nutrients, and figwort can improve the digestive condition of chickens, so that chickens can better absorb nutrients in feed and enhance the physical fitness of chickens. Astragalus contains astragalus polysaccharides and flavonoids, which have certain antiviral effects, can regulate the immune function of sick chickens, improve the mental state and stress resistance of sick chickens and promote the recovery of sick chickens.

[0008] The invention takes perilla and zanthoxylum as auxiliary medicines. After being infected with Newcastle disease, sick chickens often have respiratory symptoms such as coughing and asthma. Perilla has the effects of regulating qi, broadening the middle, promoting the lung and reducing phlegm, can relieve the spasm of respiratory smooth muscle, reduce the frequency and degree of coughing, alleviate the symptoms of asthma and keep the respiratory tract unobstructed. Zanthoxylum is rich in trace elements and coumarin with a sweet fragrance, which can increase the appetite of chickens and improve the palatability of feed, thereby helping to maintain the normal physiological functions and metabolic levels of chickens, promoting the development of immune organs and the generation of immune cells and enhancing the immunity of chickens.

[0009] The invention takes borneol and natural camphor as leading medicines. Borneol has a pungent and bitter taste and a slightly cold nature, and is attributed to the heart, spleen and lung channels. It has the nature of pungent and wandering, can guide other medicines to specific meridians and organs and enhance the targeting effect of medicines. Natural camphor has a hot nature and a pungent taste, and is attributed to the heart and spleen channels. It has good local stimulation and penetration, and can promote the penetration and absorption of medicines.

[0010] In summary, the invention utilizes the synergistic effect of each raw material component to prepare a traditional Chinese medicine composition for preventing and treating Newcastle disease and effectively improving the immunity of chickens. The raw materials of the traditional Chinese medicine composition are simple and easy to obtain, the cost is low and the effect is remarkable. The preparation process of the traditional Chinese medicine preparation is simple and the cost is low, which effectively makes up for the defects of the prior art, such as complicated preparation process, high cost and insignificant effect of the traditional Chinese medicine preparation for preventing and treating Newcastle disease, and provides a new design idea for preventing and treating Newcastle disease in chickens.

[0011] Preferably, the antiviral traditional Chinese medicine composition comprises the following components by mass fraction: garlic 12-18 parts, schizonepeta 18-22 parts, artemisia 5-8 parts, radix isatidis 5-8 parts, honeysuckle 5-8 parts, loquat leaf 5-8 parts, rattan fig 3-7 parts, astragalus 2-3 parts, perilla 2-3 parts, zanthoxylum 2-3 parts, borneol 2-3 parts, and natural camphor 1-2 parts.

[0012] The second aspect of the present application provides an antiviral traditional Chinese medicine preparation comprising the antiviral traditional Chinese medicine composition.

[0013] Preferably, the traditional Chinese medicine preparation further comprises an emulsifier and a preservative.

[0014] Preferably, the dosage form of the traditional Chinese medicine preparation is any one of oral liquid, granules, tablets, spray, or dripping pills.

[0015] The third aspect of the present application provides a preparation method of the antiviral traditional Chinese medicine preparation, comprising the following steps: Step one, according to the design ratio, weigh garlic, schizonepeta, artemisia, and radix isatidis, mix, crush, soak in the first eutectic solvent, ultrasonic treatment, solid-liquid separation, and obtain the monarch drug extract; Step two, according to the design ratio, weigh the remaining raw material components except borneol and natural camphor, mix evenly, crush, sieve, and obtain a mixture; immerse the mixture in the second eutectic solvent, ultrasonic treatment, solid-liquid separation, and obtain a mixed extract; Step three, dissolve borneol, natural camphor, and emulsifier in an alcohol solution, mix evenly, and obtain a composite emulsion; mix the monarch drug extract, the mixed extract, and the composite emulsion evenly, add a preservative, concentrate to a relative density of 1.10-1.20, sterilize, and obtain the antiviral traditional Chinese medicine preparation.

[0016] The present application uses two different eutectic solvents as extraction solvents, which can form a biphasic extraction system to realize the simultaneous extraction and separation of different polar components, effectively improve the extraction rate and extraction precision of effective components of traditional Chinese medicine, further enhance the synergistic effect between traditional Chinese medicine components, and more easily exert the efficacy.

[0017] Preferably, in step one, the first eutectic solvent is glucose and lactic acid in a molar ratio of 1.5:1-2:1.

[0018] Preferably, in step one, the preparation method of the first eutectic solvent comprises the following steps: mix glucose and lactic acid according to the design ratio, stir at 65-75°C until the solution is clear and transparent, and obtain the first eutectic solvent.

[0019] The eutectic solvent composed of glucose and lactic acid has unique molecular structure and physical and chemical properties, and has good solubility for various components in traditional Chinese medicine, such as flavonoids, polyphenols, polysaccharides and the like; the combination of the two can form strong intermolecular forces with these components, promote the dissolution of components from traditional Chinese medicine raw materials, and thus improve the extraction efficiency. Moreover, the eutectic solvent can be decomposed by microorganisms and will not accumulate in the environment, which makes it have less impact on the ecological environment and is more green and environmentally friendly in the process of extracting components from traditional Chinese medicine.

[0020] Preferably, in step one, the mass-volume ratio of the total mass of garlic, catnip, artemisia and isatis root to the mass of the first eutectic solvent is 1g:5mL-1g:7mL.

[0021] Preferably, in step one, the soaking time is 3h-5h.

[0022] Preferably, in step one, the ultrasonic treatment time is 10min-20min.

[0023] Preferably, in step one, the ultrasonic treatment power is 30kW-40kW.

[0024] Preferably, in step two, the second eutectic solvent is choline chloride, malic acid and water with a molar ratio of 1:1:2-1:1:4.

[0025] Preferably, in step two, the preparation method of the second eutectic solvent comprises the following steps: mixing choline chloride, malic acid and water according to the designed ratio, stirring at 70-80℃ until the solution is clear and transparent, and obtaining the second eutectic solvent.

[0026] Choline chloride is a naturally occurring nutrient, and malic acid is also an intermediate product in the metabolic process of living organisms, both of which have good biocompatibility; moreover, this eutectic solvent has good solubility for various components in traditional Chinese medicine, can dissolve flavonoids, alkaloids, polysaccharides and other active ingredients, and its unique molecular structure and hydrogen bond network can form strong interaction with traditional Chinese medicine components, promote the dissolution of components, and improve the extraction efficiency.

[0027] Preferably, in step two, the mass-volume ratio of the mixture to the second eutectic solvent is 1g:5mL-1g:10mL.

[0028] Preferably, in step two, the ultrasonic treatment time is 20min-30min.

[0029] Preferably, in step two, the ultrasonic treatment power is 35kW-40kW.

[0030] Preferably, in step three, the emulsifier is Tween 80.

[0031] Preferably, in step three, the mass ratio of said borneol to emulsifier is 1:3-1:8.

[0032] Preferably, in step three, said preservative is benzoate or sorbate.

[0033] Further preferably, in step three, said preservative is any one or more of sodium benzoate, sodium sorbate or potassium sorbate.

[0034] Preferably, in step three, said alcohol solution is an ethanol solution with a mass concentration of 45%-55%.

[0035] Preferably, in step three, the mass-volume ratio of said borneol to said alcohol solution is 1g:12mL-1g:25mL.

[0036] Preferably, in step three, the added amount of said preservative is 0.4wt%-0.5wt% of the mass of said compound emulsion.

[0037] Preferably, in step three, the sterilization temperature is 110℃-123℃, and the sterilization time is 25min-45min.

[0038] To sum up, the present application uses garlic, catnip, artemisia annua and isatis root as monarch drugs, uses honeysuckle, loquat leaf, randia dumetorum and astragalus as minister drugs, uses perilla and zanthoxylum as auxiliary drugs, and uses borneol and natural camphor as minister drugs, and a traditional Chinese medicine composition for clearing away pathogenic factors and resisting viruses is prepared by using the relationship of monarch, minister, auxiliary and minister. The traditional Chinese medicine composition has simple formula and low-cost raw materials. Furthermore, the present application also uses the traditional Chinese medicine composition as a main raw material to prepare a traditional Chinese medicine preparation for clearing away pathogenic factors and resisting viruses. The traditional Chinese medicine preparation for clearing away pathogenic factors and resisting viruses has simple process and low cost, effectively makes up for the deficiencies of the prior art, such as complicated preparation process, high cost and insignificant drug efficacy, and provides a new design idea for preventing and treating newcastle disease of chickens. DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0040] The preparation method of the first eutectic solvent in the following examples and comparative examples includes the following steps: According to the molar ratio of 1.8:1, glucose and lactic acid are weighed and mixed, and stirred at 70℃ until the solution is clear and transparent, to obtain the first eutectic solvent.

[0041] The preparation method of the second eutectic solvent in the following examples and comparative examples comprises the following steps: Choline chloride, malic acid and water are weighed according to a molar ratio of 1:1:3, mixed, and stirred at 75°C until the solution is clear and transparent to obtain the second eutectic solvent.

[0042] Example 1 The present example provides a traditional Chinese medicine composition for clearing away epidemic pathogens and resisting viruses and a preparation method thereof, which specifically comprises the following contents: The traditional Chinese medicine composition for clearing away epidemic pathogens and resisting viruses comprises the following raw material components in mass fraction: garlic 18g, schizonepeta 22g, artemisia 8g, radix isatidis 7g, honeysuckle 8g, loquat leaf 8g, rattan fig 6g, astragalus 4g, perilla 3g, zanthoxylum 3g, borneol 3g and natural camphor 3g.

[0043] The preparation method of the traditional Chinese medicine composition for clearing away epidemic pathogens and resisting viruses comprises the following steps: Step one, the garlic, schizonepeta, artemisia and radix isatidis are weighed according to the designed ratio, mixed, crushed, soaked in 330mL of the first eutectic solvent for 3.5h, ultrasonically treated, and solid-liquid separated to obtain the monarch drug extract; Step two, the remaining raw material components except borneol and natural camphor are weighed according to the designed ratio, mixed uniformly, crushed, and sieved to obtain a mixture; the mixture is immersed in 250mL of the second eutectic solvent, ultrasonically treated, and solid-liquid separated to obtain a mixed extract; Step three, the borneol, natural camphor and 15g of Tween 80 are dissolved in 50mL of an ethanol solution with a mass concentration of 50%, mixed uniformly to obtain a composite emulsion; the monarch drug extract, the mixed extract and the composite emulsion are mixed uniformly, 0.3g of sodium benzoate is added, concentrated to a relative density of 1.12, and sterilized at 120°C for 30min to obtain the traditional Chinese medicine preparation for clearing away epidemic pathogens and resisting viruses.

[0044] Example 2 The present example provides a traditional Chinese medicine composition for clearing away epidemic pathogens and resisting viruses and a preparation method thereof, which specifically comprises the following contents: The traditional Chinese medicine composition for clearing away epidemic pathogens and resisting viruses comprises the following raw material components in mass fraction: garlic 20g, schizonepeta 15g, artemisia 6g, radix isatidis 10g, honeysuckle 5g, loquat leaf 7g, rattan fig 8g, astragalus 3g, perilla 3g, zanthoxylum 3g, borneol 3g and natural camphor 2g.

[0045] The preparation method of the traditional Chinese medicine composition for clearing away epidemic pathogens and resisting viruses comprises the following steps: Step one, the garlic, schizonepeta, artemisia and radix isatidis are weighed according to the designed ratio, mixed, crushed, soaked in 330mL of the first eutectic solvent for 3.5h, ultrasonically treated, and solid-liquid separated to obtain the monarch drug extract; Step two, according to the design of the proportion of the remaining ingredients except bomeol and natural camphor, mixed evenly, crushing, sieving, get the mixture; the mixture is immersed in 250 mL of the second eutectic solvent, ultrasonic treatment, solid-liquid separation, get mixed extract; Step three, bomeol, natural camphor and 15 g of Tween 80 is dissolved in 50 mL of 50% ethanol solution, mixed evenly, get composite emulsion; the monarch drug extract, mixed extract and composite emulsion are mixed evenly, 0.3 g of sodium benzoate is added, concentrated to the relative density of the medicinal liquid is 1.11, sterilized at 122℃ for 25 min, get Qingwen anti-virus traditional Chinese medicine preparation.

[0046] Example 3 The present embodiment provides a Qingwen anti-virus traditional Chinese medicine composition and a preparation method thereof, which specifically includes the following contents: The Qingwen anti-virus traditional Chinese medicine composition includes the following raw material components in mass fraction: garlic 11 g, schizonepeta 25 g, artemisia 10 g, radix isatidis 5 g, honeysuckle 6 g, loquat leaf 6 g, rattan fig 5 g, astragalus 4 g, perilla 3 g, zanthoxylum 3 g, bomeol 3 g and natural camphor 2 g.

[0047] The preparation method of the Qingwen anti-virus traditional Chinese medicine composition includes the following steps: Step one, according to the design of the proportion of garlic, schizonepeta, artemisia and radix isatidis, mixed, crushing, soaking in 330 mL of the first eutectic solvent for 3.5 h, ultrasonic treatment, solid-liquid separation, get the monarch drug extract; Step two, according to the design of the proportion of the remaining ingredients except bomeol and natural camphor, mixed evenly, crushing, sieving, get the mixture; the mixture is immersed in 250 mL of the second eutectic solvent, ultrasonic treatment, solid-liquid separation, get mixed extract; Step three, bomeol, natural camphor and 15 g of Tween 80 is dissolved in 50 mL of 50% ethanol solution, mixed evenly, get composite emulsion; the monarch drug extract, mixed extract and composite emulsion are mixed evenly, 0.3 g of sodium benzoate is added, concentrated to the relative density of the medicinal liquid is 1.10, sterilized at 123℃ for 25 min, get Qingwen anti-virus traditional Chinese medicine preparation.

[0048] Comparative example 1 The present comparative example provides a Qingwen anti-virus traditional Chinese medicine composition and a preparation method thereof, which is different from example 1 in that: the garlic is replaced by an equal amount of wind-preventing, and the other ingredients and preparation process remain unchanged, which is not described here.

[0049] Comparative example 2 The counter example provides a traditional Chinese medicine composition for clearing away epidemic pathogens and resisting viruses and a preparation method thereof, which is different from example 1 in that artemisia argyi is replaced by an equal amount of artificial bezoar, and other ingredients and preparation processes are unchanged, and details are not repeated here.

[0050] Counter example 3 The counter example provides a traditional Chinese medicine composition for clearing away epidemic pathogens and resisting viruses and a preparation method thereof, which is different from example 1 in that artemisia argyi is replaced by an equal amount of artificial bezoar, and other ingredients and preparation processes are unchanged, and details are not repeated here.

[0051] Counter example 4 The counter example provides a traditional Chinese medicine composition for clearing away epidemic pathogens and resisting viruses and a preparation method thereof, which is different from example 1 in that artemisia argyi is replaced by an equal amount of artificial bezoar, and other ingredients and preparation processes are unchanged, and details are not repeated here.

[0052] Test example The test example provides a traditional Chinese medicine granule for clearing away epidemic pathogens and resisting viruses, which specifically includes the following contents. 15 g of the traditional Chinese medicine composition for clearing away epidemic pathogens and resisting viruses provided in each of the examples or counter examples is added with 45 g of medicinal starch, 1 g of sodium carboxymethyl cellulose and 0.5 g of β-cyclodextrin, and then mixed uniformly to obtain the traditional Chinese medicine granule for clearing away epidemic pathogens and resisting viruses.

[0053] Other traditional Chinese medicine preparations are prepared according to conventional preparation methods, and details are not repeated here.

[0054] In order to further embody the technical effects of the present application, the traditional Chinese medicine granule prepared by using the test example 1 is tested as follows: (1) Curative effect test on chickens artificially infected with newcastle disease vaccine: 300 one-day-old test chickens are taken, normally raised, and blood is collected once every 3 days to separate serum and determine the titer (HI) of newcastle disease antibody in the serum. The chickens are divided into 6 groups, namely, treatment group I, treatment group II, treatment group III, drug control group, positive control group and blank control group, with 50 chickens in each group. The traditional Chinese medicine granule for clearing away epidemic pathogens and resisting viruses provided in example 1 is added into clean drinking water at a dose of 0.5 g per chicken (low dose), 1.0 g per chicken (medium dose) and 1.5 g per chicken (high dose) for the treatment group I, the treatment group II and the treatment group III respectively, and the chickens are given the medicine twice, by taking the clean drinking water in the morning and evening, for 5 days. The drug control group is given 0.2 mg of mefenamine per chicken per day, and the time and method are the same as those of the previous three groups. The positive control group is only modeled without being given medicine. The blank control group is not modeled without being given medicine.

[0055] The experiment was carried out after the chicks were raised for 1 week, that is, at the age of 21 days, the chicks were inoculated with Newcastle disease vaccine ND-IV system (ND-LaSota), and the vaccine was injected into the chicks at 0.5 mL per chick. The main symptoms of the modeling success are as follows: depression, gradually decreased appetite, rough and messy feathers, drowsiness, yellowish green and watery feces, swollen crop, a large amount of mucus flowing out of the oral cavity of the sick chicken, open-mouth breathing, and a small number of sick chickens showing neurological symptoms. The clinical symptoms and pathological changes of the chickens in each group were observed, and the severity was compared. The number of cases, recovery, and death was recorded, the time experienced by each group to show symptoms (i.e., incubation period) was observed, and the observation was ended after 15 days.

[0056] Table 1 shows the results of the curative effect test of artificially infected chickens with Newcastle disease vaccine

[0057] As can be seen from Table 1, compared with the positive control group, the number of deaths in the treatment group after administration was greatly reduced, and the number of recoveries was higher. Among them, the treatment effect of the middle dose group was the best, which could reach the level of the drug control group. Therefore, the middle dose group was used for subsequent testing.

[0058] (2) Clinical test Test animals: Newcastle disease-infected laying hens raised in a chicken farm in Xingtai, Hebei, at the age of 30 days.

[0059] Symptoms: depression, gradually decreased appetite, rough and messy feathers, drowsiness, yellowish green and watery feces, swollen crop, a large amount of mucus flowing out of the oral cavity of the sick chicken, open-mouth breathing, and a small number of sick chickens showing neurological symptoms.

[0060] Test grouping: The sick chickens were divided into 6 groups, with 100 sick chickens in each group. The chickens in each group were fed with the same feed, and the feeding and management were the same except for the different drugs.

[0061] Test drugs: the Qingwen anti-virus traditional Chinese medicine granules provided by Example 1 of the present application, the Qingwen anti-virus traditional Chinese medicine granules provided by Comparative Example 1 of the present application, the Qingwen anti-virus traditional Chinese medicine granules provided by Comparative Example 2 of the present application, the Qingwen anti-virus traditional Chinese medicine granules provided by Comparative Example 3 of the present application, the Qingwen anti-virus traditional Chinese medicine granules provided by Comparative Example 4 of the present application, and the control group.

[0062] Test processing: Test group: The drug was added to clean drinking water at a dose of 1.0 g per chicken (middle dose), and the drug was administered in two times. The chickens were allowed to drink freely in the morning and evening, and the administration was continued for 5 days. Control group: No drug was administered.

[0063] Test symptom observation: During the test period, the development of the disease of the chickens in each group was recorded, and the mental state, appetite, comb color, and behavior state of the sick chickens were observed.

[0064] Efficacy evaluation: cure: after administration, the spirit, food intake and water basically return to normal, defecation is normal, can walk normally, smooth feathers, comb presents normal light red, oral cavity without mucus, normal breathing, normal crop.

[0065] Effective: after administration, the spirit, food intake and water have obvious improvement, defecation is improved, can walk normally, smooth feathers, comb presents normal light red or light gray, oral cavity without mucus, normal breathing, a few breathe with mouth, normal crop.

[0066] Invalid: after administration, the symptoms do not improve or even get worse.

[0067] Test results: as shown in Table 2.

[0068] Table 2 clinical test results

[0069] According to Table 2, it can be seen that the Qingwen antiviral traditional Chinese medicine granules provided by the embodiment 1 of the present application have excellent effect of treating newcastle disease, and the cure rate can reach 93%, which effectively solves the problems of complicated preparation process, high cost and insignificant efficacy of the Qingwen antiviral traditional Chinese medicine preparation in the prior art.

[0070] The above only describes the preferred embodiments of the present application, and is not intended to limit the present application, and any modification, equivalent replacement or improvement made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A traditional Chinese medicine composition for treating epidemic febrile diseases and resisting viruses, characterized in that: The raw material components include garlic 10-20 parts, catnip 15-25 parts, artemisia 5-10 parts, radix isatidis 5-10 parts, honeysuckle 5-10 parts, loquat leaves 5-10 parts, figwort 1-10 parts, milkvetch 1-5 parts, perilla 1-5 parts, zanthoxylum 1-5 parts, borneol 1-5 parts, and natural camphor 1-5 parts. ​ 2. The traditional Chinese medicine composition for treating epidemic febrile diseases and resisting viruses according to claim 1, characterized in that: The raw material components include garlic 12-18 parts, catnip 18-22 parts, artemisia 5-8 parts, radix isatidis 5-8 parts, honeysuckle 5-8 parts, loquat leaves 5-8 parts, figwort 3-7 parts, milkvetch 2-3 parts, perilla 2-3 parts, zanthoxylum 2-3 parts, borneol 2-3 parts, and natural camphor 2-3 parts.

3. A traditional Chinese medicine preparation for clearing away pathogenic factors and resisting viruses, characterized in that: The antipyretic and anti-virus traditional Chinese medicine composition comprises the antipyretic and anti-virus traditional Chinese medicine composition according to any one of claims 1 or 2.

4. The antiviral traditional Chinese medicine preparation of claim 3, characterized in that: The traditional Chinese medicine preparation further comprises an emulsifier and a preservative.

5. The antipyretic and antiviral traditional Chinese medicine preparation of claim 3 or 4, characterized in that: The dosage form of the traditional Chinese medicine preparation is any one of oral liquid, granules, tablets, spray, or drop pills.

6. A method for preparing the antiviral traditional Chinese medicine preparation according to any one of claims 3-5, characterized in that: The method comprises the following steps: Step one, according to the designed ratio, garlic, catnip, artemisia, and radix isatidis are weighed and mixed, crushed, soaked in a first eutectic solvent, ultrasonically treated, and solid-liquid separated to obtain a monarch herb extract; Step two, according to the designed ratio, the remaining raw material components except borneol and natural camphor are weighed and uniformly mixed, crushed, and sieved to obtain a mixture; the mixture is immersed in a second eutectic solvent, ultrasonically treated, and solid-liquid separated to obtain a mixed extract; Step three, borneol, natural camphor, and an emulsifier are dissolved in an alcohol solution, uniformly mixed to obtain a composite emulsion; the monarch herb extract, the mixed extract, and the composite emulsion are uniformly mixed, a preservative is added, concentrated to a relative density of 1.10-1.20, sterilized to obtain an antipyretic and anti-virus traditional Chinese medicine preparation.

7. The method for preparing the traditional Chinese medicine preparation for dispelling epidemic pathogens and resisting viruses according to claim 6, characterized in that: In step one, the first eutectic solvent is glucose and lactic acid at a molar ratio of 1.5:1-2:1; and / or In step one, the mass-volume ratio of the total mass of garlic, catnip, artemisia, and radix isatidis to the mass of the first eutectic solvent is 1g:5mL-1g:7mL; and / or In step one, the soaking time is 3h-8h.

8. The method for preparing the traditional Chinese medicine preparation for dispelling epidemic pathogens and resisting viruses according to claim 6, characterized in that: In step two, the second eutectic solvent is choline chloride, malic acid, and water at a molar ratio of 1:1:2-1:1:4; and / or In step two, the mass-volume ratio of the mixture to the second eutectic solvent is 1g:5mL-1g:10mL; and / or In step two, the ultrasonic treatment time is 20min-30min.

9. The method for preparing the traditional Chinese medicine preparation for dispelling epidemic pathogens and resisting viruses according to claim 6, characterized in that: In step three, the emulsifier is Tween 80; and / or In step three, the mass ratio of borneol to the emulsifier is 1:3-1:8; and / or In step three, the preservative is benzoate or sorbate; and / or In step three, the alcohol solution is an ethanol solution with a mass concentration of 45%-55%; and / or In step three, the mass-volume ratio of borneol to the alcohol solution is 1g:12mL-1g:18mL; and / or In step three, the addition amount of the preservative is 0.4wt%-0.5wt% of the mass of the composite emulsion.

10. The method for preparing the traditional Chinese medicine preparation for dispelling epidemic pathogens and resisting viruses according to claim 6, characterized in that: In step three, the sterilization temperature is 110°C to 123°C and the sterilization time is 25 minutes to 45 minutes. In step three, the sterilization temperature is 110°C to 123°C and the sterilization time is 25 minutes to 45 minutes.

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