Method for purifying sweet protein Brazzein
By employing steps such as high-speed centrifugation, D201 resin treatment, activated carbon filtration, three-stage plate and frame filtration, and 3kDa membrane filtration, combined with freeze drying, the problem of low purification efficiency of carbapenem in traditional methods has been solved, achieving high-purity and efficient extraction of carbapenem, which is suitable for the food industry.
CN121362235APending Publication Date: 2026-01-20FUZHOU DAOFU BIOTECHNOLOGY CO LTD
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Patent Information
- Application Number
- CN202510778590.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2024-10-25
- Filing Date
- 2025-06-11
- Publication Date
- 2026-01-20
AI Technical Summary
Technical Problem
Traditional methods are difficult to use efficiently and cost-effectively to purify brassin from Trichoderma reesei fermentation broth, remove impurities and pigments, and thus affect its application in the food industry.
Method used
The high-purity extraction of Brazilian sweet protein is achieved by employing steps such as high-speed centrifugation, D201 resin treatment, activated carbon filtration, three-stage plate and frame filtration, 3kDa membrane filtration, and pH-adjusted heating centrifugation, combined with freeze drying.
Benefits of technology
It effectively removes pigments, inorganic salts, and impurities from the fermentation broth, yielding high-purity Brazilian sweet protein, which is suitable as a sweetener in the food industry.
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Abstract
The invention relates to the technical field of biology, in particular to a method for purifying sweet protein Brazzein. The invention provides a method for purifying sweet protein Brazzein, which can effectively remove pigments, inorganic salts and impure proteins to obtain high-purity Brazzein. In specific implementation, fermentation liquor comes from trichoderma reesei engineering bacteria with high yield of Brazzein, solid-liquid separation is realized through multiple times of high-speed centrifugation, and miscellaneous proteins and pigments are removed by using D201 resin and activated carbon after the pH value is adjusted. Residual activated carbon is removed through three-stage filtration, and a crude Brazilian sweet protein extracting solution is obtained. And filtering and concentrating by using a 3kDa membrane, carrying out high-speed centrifugation after heating treatment, collecting a supernatant, and finally freeze-drying to obtain the brazitame protein freeze-dried powder. Experimental results show that the method can obviously improve the purity of the target protein and obviously improve the concentration of the target protein.
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