Apparatus for extracting benzothiazole compounds from ryegrass hydroponic solution

Abstract

The present application relates to experimental extraction device technical field, specifically, it relates to the extraction device of benzothiazole compound in ryegrass hydroponics, including culture solution processing bin and SPE column, it also includes the material conveying mechanism for transporting SPE column, a plurality of liquid storage tubes and extrusion device arranged above the SPE column. The extraction device of benzothothiazole compound in ryegrass hydroponics and its method, through the engagement transmission structure of moving toothed plate and external gear, in the process of slider transverse transfer SPE column, synchronous drive the internal structure rotation of stirring part, the three-dimensional stirring net formed can realize radial disturbance and axial liquid flow circulation simultaneously, and before hydroponics transfer, real-time break particle agglomeration, prevent the solid phase filler from being blocked by precipitation, dynamically enhance benzothiazole mass transfer efficiency, improve its recovery rate.

Description

Technical Field

[0001] This invention relates to the field of experimental extraction apparatus technology, and more specifically, to an extraction apparatus for benzothiazole compounds in ryegrass hydroponic solution. Background Technology

[0002] In the hydroponic system of ryegrass, benzothiazole compounds are allelochemicals or metabolites secreted by the roots. Their extraction and separation are key prerequisites for conducting bioactivity studies. Due to the complex composition of the hydroponic solution, the target compounds often exist in trace amounts and are easily interfered with by humic acid, inorganic salts, etc. Therefore, it is necessary to achieve separation and enrichment through efficient extraction methods. Solid phase extraction (SPE) has become a commonly used technique for the extraction of such compounds because it has the advantages of selective adsorption, low solvent consumption, and simple operation.

[0003] Patent application number CN202421901027.3 discloses a solid phase extraction filling device, including a shell, a material box fixedly connected to the shell, the material box having several discharge holes, a first partition and a second partition fixedly connected inside the shell, and a feeding mechanism slidably arranged between the first partition and the second partition, which can quickly fill multiple empty column tubes to obtain a solid phase extraction column, and make the adsorbent material more uniform and compact by a vibration motor.

[0004] However, existing solid phase extraction (SPE) equipment has two major drawbacks: First, solvent transfer and flow rate control rely on manual operation or separate drive systems, requiring repeated switching between syringe pumps and vacuum devices. This not only makes the process cumbersome but also causes cross-contamination due to tubing residues. Furthermore, the manual flow rate adjustment error can be as high as ±5% (if the flow rate exceeds 2 mL / min during the activation phase, it will reduce the activation efficiency of the binding sites). Second, the sample pretreatment and extraction process are disconnected. When the hydroponic solution is left to stand, the particle sedimentation rate increases, which can clog the micropores of the SPE column solid phase packing. The external oscillation device requires independent energy consumption and increases mass transfer delay, resulting in low benzothiazole recovery rates that are difficult to meet detection requirements.

[0005] In view of this, we propose an extraction device and method for benzothiazole compounds in ryegrass hydroponic solution. Summary of the Invention

[0006] The purpose of this invention is to provide an extraction device for benzothiazole compounds in ryegrass hydroponic solution, which uses an electric actuator to drive a toothed plate to complete multiple lifting and lowering actions, thereby solving the problems mentioned in the background art.

[0007] To achieve the above objectives, the present invention provides the following technical solution:

[0008] An extraction device for benzothiazole compounds in ryegrass hydroponic solution includes a solution treatment chamber and an SPE column, as well as a material conveying mechanism for transporting the SPE column, several liquid storage pipes disposed above the SPE column, and an extrusion device.

[0009] The material conveying mechanism includes an electric push rod and a toothed plate at its top.

[0010] The liquid storage tube includes a fixed tube body, an insert rod located below the fixed tube body, and a bent plate sleeved on the outer end of the insert rod. A stirring part is also provided inside the liquid storage tube located in the middle. A cross tube body is snapped into the bottom end of the fixed tube body. The insert rod slides inside the cross tube body. A through hole is opened inside the rod, and a protruding rod is integrally formed at the end. A sloping groove is opened at the bottom of the bent plate for the protruding rod to slide.

[0011] This setting allows the upper part of the bending plate to slide and engage with the side of the toothed plate. The electric actuator controls the toothed plate to move the bending plate upward, allowing the protruding rod to move along the inclined groove. This drives the insertion rod to move laterally, allowing the through hole to connect with the vertical tube of the cross tube body. This allows the solution inside the fixed tube body to drip into the SPE column. Furthermore, when the toothed plate moves laterally, it can also drive the stirring part to stir the solution in the middle liquid storage tube.

[0012] The extrusion device includes a pair of racks, an internal gear disposed between the pair of racks, and a piston plate that moves subsequently on the side racks;

[0013] This setting also causes the electric actuator to control the toothed plate to move the front toothed plate upward, while the meshing internal gear drives the rear rack to move downward, allowing the piston plate to discharge the SPE column solution at a fixed flow rate.

[0014] In the technical solution of the present invention, the culture medium treatment chamber includes a chamber body, a fixed frame welded to the outer wall of the chamber body, a partition plate disposed below the fixed frame and welded to the chamber body, a number of limiting frames regularly snapped onto the outer wall of the chamber body, a number of sealing plugs snapped onto the top surface of the chamber body, and a bent plate welded to the front wall of the chamber body. A number of regularly placed glass containers are also placed on the inner bottom surface of the chamber body.

[0015] In the technical solution of the present invention, the interior of the chamber is provided with a through groove running from front to back, and the chamber is also provided with a placement groove on the front side of the groove. The partition is fitted with a funnel directly above several glass containers. The positions of several limiting frames correspond to the positions of several squeezing devices. Several sealing plugs are located directly above the liquid storage tube.

[0016] The above setup integrates the solvents used in the extraction process of thiazole compounds, providing a platform and, together with the material conveying mechanism, laying the foundation for solid-phase extraction of thiazole compounds.

[0017] In the technical solution of the present invention, the material conveying mechanism further includes a motor, a lead screw coaxially connected to its output shaft, and a slider sleeved on the outside of the lead screw and slidably connected to the slide groove and the placement groove. The electric push rod is fixedly connected to the top surface of the slider by screws. A plate surface groove is opened on the outer wall of the toothed plate, and the upper end of the bent plate is slidably engaged with the plate surface groove.

[0018] This feature addresses three major issues in traditional SPE equipment caused by manual operation: positioning deviation, timing chaos, and contamination risk.

[0019] In the technical solution of the present invention, the SPE column includes a column body inserted into the end of the slider plate, a solid packing material placed inside the column body for capturing benzothiazole compounds, and two filter screens snapped into the inside of the column body, the two filter screens being located in the upper and lower directions of the solid packing material.

[0020] This setting provides the basis for the capture of benzothiazole compounds.

[0021] In the technical solution of the present invention, the liquid storage tube further includes a first spring adhered to the rear end of the insertion rod, the fixed tube body is snapped onto the inner bottom surface of the fixed frame, and the other end of the first spring is adhered to the tube wall of the cross tube body.

[0022] In the technical solution of the present invention, the bending plate is slidably connected to the inside of the limiting frame, the inclined groove is set with the front higher than the back, and the size of the top horizontal plate of the bending plate is smaller than the size of the groove on the plate surface.

[0023] The above setup eliminates solvent residue and reduces cross-contamination between different solutions by using the transverse sealing reset of the insert rod in conjunction with the extrusion device.

[0024] In the technical solution of the present invention, the stirring part includes an external gear, a rotating shaft engaged inside the external gear, and two stirring blades that rotate with the rotating shaft. It also includes a connecting rod connected between the two stirring blades, a round rod rotatably connected to the outside of the connecting rod, and a stirring ring rotatably connected to the top of the round rod. The stirring ring is slidably connected to the inside of the fixed tube, and the external gear meshes with the toothed plate.

[0025] This setting breaks up particle aggregation, enhances mass transfer, and improves benzothiazole capture efficiency.

[0026] In the technical solution of the present invention, the extrusion device further includes a slide rod that is engaged with the end of the rear rack cross plate and a second spring sleeved on the outside of the slide rod. The two racks are centrally symmetrically distributed. The internal gear meshes with the two racks. The size of the bottom cross plate of the rack on the front side is smaller than the size of the groove on the plate surface. The piston plate is engaged with the bottom end of the slide rod. The elastic force provided by the second spring pushes the rear rack to move upward.

[0027] This setting allows for adjustment of the dimensions of a pair of racks and internal gears according to different steps, ensuring that different solvents can flow through the SPE column at the corresponding flow rates.

[0028] On the other hand, the present invention also provides a method for extracting benzothiazole compounds from ryegrass hydroponic solution, using the above-mentioned extraction apparatus for benzothiazole compounds from ryegrass hydroponic solution, comprising the following steps:

[0029] S1. First, the operator filters the ryegrass hydroponic solution through a filter membrane, adjusts the pH value of the ryegrass hydroponic solution, and adds an internal standard of deuterated benzothiazole to the adjusted ryegrass hydroponic solution to correct the recovery rate.

[0030] S2. Next, the operator first pours methanol, ultrapure water, treated ryegrass hydroponic solution, weak solvent, and strong solvent into several storage tubes from left to right, and then places the SPE column into the round hole at the end of the slider.

[0031] S3. Then, start the motor, drive the lead screw to rotate and make the slider move laterally, and make the electric push rod move laterally together with the toothed plate to send the SPE column to the bottom of the storage tube containing methanol.

[0032] S4. The electric actuator moves the toothed plate upward, and the top horizontal plate of the bent plate located in the groove on the plate surface moves accordingly. This causes the protrusion at the end of the insert rod to move along the inclined groove, moving the insert rod laterally within the cross tube body. After the through hole connects with the vertical tube of the cross tube body, methanol is dripped into the interior of the SPE column.

[0033] S5. Next, control the electric actuator to reset the toothed plate and bending plate, and start the motor to send the SPE column to the bottom of the leftmost extrusion device.

[0034] S6. After the electric actuator moves up again, the front rack moves up and drives the rear rack to move down through the meshing internal gear, which in turn drives the slide rod and the piston plate at its bottom to move down, allowing methanol to drip from the SPE column at a fixed flow rate, thereby ensuring the solid packing is wetted, opening the bonding sites, and activating the solid packing.

[0035] S7. Next, repeat the above operation to allow ultrapure water to flow through the solid packing material, displacing the activation solvent and making the internal environment of the solid packing material consistent with the treated ryegrass hydroponic solution.

[0036] S8. During the process of controlling the motor to drive the toothed plate to move laterally, the toothed plate will mesh with the external gear, drive the rotating shaft to rotate, and make the stirring blade and the connecting rod between them rotate inside the ryegrass hydroponic solution. At the same time, through the round rod, the stirring ring will be driven to disturb the solution up and down, thereby breaking up particle agglomeration, enhancing mass transfer, and improving the benzothiazole capture efficiency.

[0037] S9. After that, the toothed plate rises and falls again, and the ryegrass hydroponic solution flows through the solid packing material. The benzothiazole compounds inside are selectively adsorbed onto the solid packing material, thus completing the sample loading operation.

[0038] S10. Next, the hydrophilic interfering substances on the solid packing are first eluted with a weak solvent, and then the benzothiazole compound is desorbed from the solid packing with a strong solvent.

[0039] S11. Finally, the operator takes the glass container on the far right and puts it into the nitrogen evaporator to enrich the benzothiazole compound, and then stores it.

[0040] Compared with the prior art, the beneficial effects of the present invention are:

[0041] 1. The extraction device and method for benzothiazole compounds in ryegrass hydroponic solution, wherein the toothed plate is driven by an electric actuator to complete a single lifting action, allowing the solution to drip from different storage tubes. At the same time, it can also form a speed-changing mechanism with a pair of racks and internal gears of the extrusion device, converting the vertical movement of the toothed plate into the uniform downward pressure of the piston plate. In actual use, by adjusting the size of the pair of racks and internal gears in the extrusion device, it is ensured that different solvents can flow through the SPE column at the corresponding flow rate, thereby eliminating the error of manual operation and achieving precise flow rate control for steps such as methanol activation, water balance, and sample loading.

[0042] 2. The extraction device and method for benzothiazole compounds in ryegrass hydroponic solution utilizes a moving toothed plate and an external gear meshing transmission structure to synchronously drive the internal structure of the stirring section to rotate during the transverse transfer of the SPE column by the slider. The resulting three-dimensional stirring net can simultaneously achieve radial disturbance and axial liquid circulation. Before the hydroponic solution is transferred, it breaks up particle agglomeration in real time, prevents precipitation from clogging the solid phase filler, dynamically enhances the mass transfer efficiency of benzothiazole, and improves its recovery rate. Attached Figure Description

[0043] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0044] Figure 2 This is a cross-sectional view of the overall structure of the present invention;

[0045] Figure 3 This is a cross-sectional schematic diagram of the culture medium treatment chamber in this invention;

[0046] Figure 4 For the present invention Figure 3 An enlarged schematic diagram of part A in the middle;

[0047] Figure 5 This is a schematic diagram of the material conveying mechanism in this invention;

[0048] Figure 6 This is a schematic diagram of the toothed plate in this invention;

[0049] Figure 7 This is a cross-sectional view of the SPE column in this invention;

[0050] Figure 8 This is a cross-sectional schematic diagram of the liquid storage tube in this invention;

[0051] Figure 9 For the present invention Figure 8 Enlarged schematic diagram of part B in the middle;

[0052] Figure 10 This is a partial structural diagram of the liquid storage tube in this invention;

[0053] Figure 11 This is a schematic diagram of the stirring section in this invention;

[0054] Figure 12 This is a schematic diagram of the extrusion device in the present invention;

[0055] Explanation of reference numerals in the attached figures:

[0056] 100. Culture medium treatment chamber; 110. Chamber body; 111. Slide chute; 112. Placement trough; 120. Fixing frame; 130. Partition plate; 131. Funnel; 140. Limiting frame; 150. Sealing plug; 160. Hanging frame;

[0057] 200. Material conveying mechanism; 210. Motor; 220. Lead screw; 230. Slider; 240. Electric actuator; 250. Toothed plate; 251. Plate surface groove;

[0058] 300, SPE column; 310, column body; 320, filter screen;

[0059] 400. Liquid storage tube; 410. Fixed tube body; 411. Cross-shaped tube body; 420. Insert rod; 421. Through hole; 422. Protruding rod; 430. First spring; 440. Bending plate; 441. Inclined groove; 450. Stirring part; 451. External gear; 452. Rotating shaft; 453. Stirring blade; 454. Connecting rod; 455. Round rod; 456. Stirring ring;

[0060] 500. Extrusion device; 510. Rack; 520. Internal gear; 530. Slide rod; 540. Piston plate; 550. Second spring;

[0061] 600. Glass containers. Detailed Implementation

[0062] The technical solutions of this invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this invention, and not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of this invention without creative effort are within the scope of protection of this invention.

[0063] Please see Figures 1-4 As shown, this embodiment provides the following technical solution:

[0064] An extraction apparatus and method for benzothiazole compounds in ryegrass hydroponic solution, comprising a hydroponic solution treatment chamber 100 and an SPE column 300, and further comprising a material conveying mechanism 200 for transporting the SPE column 300, several liquid storage pipes 400 disposed above the SPE column 300, and an extrusion device 500.

[0065] Specifically, the culture medium treatment chamber 100 includes a chamber body 110, a fixing frame 120 welded to the outer wall of the chamber body 110, a partition 130 disposed below the fixing frame 120 and welded to the chamber body 110, a number of limiting frames 140 regularly snapped onto the outer wall of the chamber body 110, a number of sealing plugs 150 snapped onto the top surface of the chamber body 110, and a hanging frame 160 welded to the front wall of the chamber body 110. A number of regularly placed glass containers 600 are also placed on the inner bottom surface of the chamber body 110.

[0066] Furthermore, the interior of the silo 110 is provided with a through-flow groove 111, and the silo 110 is also provided with a placement groove 112 on the front side of the groove 111. The partition 130 is fitted with a funnel 131 directly above the glass containers 600. The positions of the limiting frames 140 correspond to the positions of the squeezing devices 500. The sealing plugs 150 are located directly above the liquid storage tube 400.

[0067] Furthermore, the chute 111 and placement groove 112 on the hopper 110 provide a sliding range for the structure in the material conveying mechanism 200, the fixed frame 120 is used to provide a placement platform for several liquid storage tubes 400 and the extrusion device 500, the funnel 131 on the partition 130 ensures that the discharged liquid can fall accurately into the glass container 600 below, and after the sealing plug 150 is opened, it is convenient for the operator to add solvent to the liquid storage tube 400. This setting integrates the solvent used in the extraction process of thiazole compounds and provides a platform, which, together with the material conveying mechanism 200, provides a basis for the solid phase extraction of thiazole compounds.

[0068] Please see Figures 5-6 As shown, in this embodiment, the material conveying mechanism 200 includes an electric push rod 240 and a toothed plate 250 at its top.

[0069] Specifically, the material conveying mechanism 200 also includes a motor 210, a lead screw 220 coaxially connected to its output shaft, and a slider 230 sleeved on the outside of the lead screw 220 and slidably connected to the slide groove 111 and the placement groove 112. The electric push rod 240 is fixedly connected to the top surface of the slider 230 by screws. The outer wall of the toothed plate 250 is provided with a plate surface groove 251, and the upper end of the bent plate 440 is slidably engaged with the plate surface groove 251.

[0070] Furthermore, after the motor 210 starts, the drive screw 220 rotates, causing the slider 230 to move laterally, while the electric push rod 240 and the toothed plate 250 move laterally together, sending the SPE column 300 to the bottom of the storage tube 400 containing different solutions. This setting solves the three major problems of positioning deviation, timing disorder and contamination risk caused by manual operation in traditional SPE equipment.

[0071] Please see Figure 7 As shown, in this embodiment, the SPE column 300 includes a column body 310 inserted into the end of the horizontal plate of the slider 230, a solid phase packing material placed inside the column body 310 for capturing benzothiazole compounds, and two filter screens 320 snapped into the inside of the column body 310. The two filter screens 320 are located in the upper and lower directions of the solid phase packing material.

[0072] Furthermore, after activation, equilibration, sample loading, and rinsing, the solid-phase packing material can capture benzothiazole compounds. The upper filter 320 is used to block suspended particles in the hydroponic solution, such as plant fibers and soil debris, to prevent them from clogging the internal micropores of the solid-phase packing material. The lower filter 320 is used to prevent fine particles such as C18 silica gel or HLB polymer from leaking out of the column 310 with the solution. This setting provides the basis for the capture of benzothiazole compounds.

[0073] Please see Figures 8-10 As shown, in this embodiment, the liquid storage tube 400 includes a fixed tube body 410, an insert rod 420 disposed below the fixed tube body 410, and a bending plate 440 sleeved on the outer end of the insert rod 420. The liquid storage tube 400 located in the middle is also provided with a stirring part 450. The bottom end of the fixed tube body 410 is clamped to a cross tube body 411. The insert rod 420 slides in the horizontal tube of the cross tube body 411. It has a through hole 421 inside and a protruding rod 422 integrally formed at the end. The bottom of the bending plate 440 has an inclined groove 441 for the protruding rod 422 to slide.

[0074] Specifically, the liquid storage tube 400 also includes a first spring 430 attached to the rear end of the insertion rod 420, the fixing tube body 410 is snapped onto the inner bottom surface of the fixing frame 120, and the other end of the first spring 430 is attached to the tube wall of the cross tube body 411.

[0075] Furthermore, the bent plate 440 is slidably connected to the inside of the limiting frame 140, the inclined groove 441 is set with the front higher than the back, and the top horizontal plate of the bent plate 440 is smaller than the size of the plate surface groove 251.

[0076] Furthermore, the upper end of the bending plate 440 is slidably engaged with the toothed plate 250 on the side. The electric actuator 240 controls the toothed plate 250 to move the bending plate 440 upward, allowing the protruding rod 422 to move along the inclined groove 441. This drives the insertion rod 420 to move laterally, allowing the through hole 421 to communicate with the vertical tube of the cross tube 411, so that the solution in the fixed tube 410 drips into the SPE column 300. The first spring 430 provides the insertion rod 420 with outward elastic force, ensuring the sealing of the insertion rod 420 to the vertical tube of the cross tube 411 under natural conditions. This setting, through the lateral sealing reset of the insertion rod 420, in conjunction with the extrusion device 500, eliminates solvent residue and reduces the cross-contamination rate between different solutions.

[0077] Please see Figures 8-11 As shown, in this embodiment, the stirring unit 450 includes an external gear 451, a rotating shaft 452 that is engaged inside the external gear 451, and two stirring blades 453 that rotate with the rotating shaft 452. It also includes a connecting rod 454 connected between the two stirring blades 453, a round rod 455 rotatably connected to the outside of the connecting rod 454, and a stirring ring 456 rotatably connected to the top of the round rod 455. The stirring ring 456 is slidably connected to the inside of the fixed tube 410, and the external gear 451 meshes with the toothed plate 250.

[0078] Furthermore, when the toothed plate 250 moves laterally, it can also drive the stirring part 450 to stir the solution in the central liquid storage tube 400. Specifically, the toothed plate 250 will mesh with the external gear 451, driving the rotating shaft 452 to rotate, causing the stirring blade 453 and the connecting rod 454 between them to rotate inside the ryegrass hydroponic solution. At the same time, the round rod 455 drives the stirring ring 456 to disturb the solution up and down. This setting breaks up particle agglomeration, enhances mass transfer, and improves the benzothiazole capture efficiency.

[0079] Please see Figures 5-12 As shown, in this embodiment, the extrusion device 500 includes a pair of racks 510, an internal gear 520 disposed between the pair of racks 510, and a piston plate 540 for subsequent movement of the racks 510.

[0080] Specifically, the extrusion device 500 also includes a slide bar 530 that is engaged with the end of the horizontal plate of the rear rack 510 and a second spring 550 that is sleeved on the outside of the slide bar 530. The two racks 510 are centrally symmetrically distributed. The internal gear 520 meshes with the two racks 510. The size of the horizontal plate at the bottom of the front rack 510 is smaller than the size of the groove 251 on the plate surface. The piston plate 540 is engaged with the bottom end of the slide bar 530. The elastic force provided by the second spring 550 pushes the rear rack 510 to move upward.

[0081] Furthermore, the electric actuator 240 controls the toothed plate 250 to move the front toothed plate 250 upward, while the meshing internal gear 520 drives the rear rack 510 downward, allowing the piston plate 540 to discharge the solution from the SPE column 300 at a fixed flow rate. This setting can be adjusted according to different steps to ensure that different solvents can flow through the SPE column at the corresponding flow rate.

[0082] This invention also provides a method for extracting benzothiazole compounds from ryegrass hydroponic solution, using the aforementioned extraction apparatus for benzothiazole compounds from ryegrass hydroponic solution, comprising the following steps:

[0083] S1. First, the operator filters the ryegrass hydroponic solution through a filter membrane, adjusts the pH value of the ryegrass hydroponic solution, and adds an internal standard of deuterated benzothiazole to the adjusted ryegrass hydroponic solution to correct the recovery rate.

[0084] S2. Next, the operator first pours methanol, ultrapure water, treated ryegrass hydroponic solution, weak solvent, and strong solvent from left to right into the interior of several storage tubes 400, and then places the SPE column 300 into the round hole at the end of the slider 230.

[0085] S3. Then, start the motor 210, drive the lead screw 220 to rotate and make the slider 230 move laterally, while the electric push rod 240 and the toothed plate 250 move laterally together, sending the SPE column 300 to the bottom of the storage tube 400 containing methanol.

[0086] S4. The electric actuator 240 moves the toothed plate 250 upward, and the top horizontal plate of the bent plate 440 located in the groove 251 on the plate surface moves accordingly. This causes the protruding rod 422 at the end of the insert rod 420 to move along the inclined groove 441, causing the insert rod 420 to move laterally within the cross tube 411. After the through hole 421 connects with the vertical tube of the cross tube 411, methanol is dripped into the interior of the SPE column 300.

[0087] S5. Then, control the electric push rod 240 to reset the toothed plate 250 and the bending plate 440, and start the motor 210 to send the SPE column 300 to the bottom of the leftmost extrusion device 500.

[0088] S6. After the electric actuator 240 moves upward again, the front rack 510 moves upward and drives the rear rack 510 to move downward through the meshing internal gear 520, which in turn drives the slide rod 530 and its bottom piston plate 540 to move downward, allowing methanol to drip from the SPE column 300 at a fixed flow rate, thereby ensuring the solid packing is wetted, opening the bonding sites, and activating the solid packing.

[0089] S7. Next, repeat the above operation to allow ultrapure water to flow through the solid packing material, displacing the activation solvent and making the internal environment of the solid packing material consistent with the treated ryegrass hydroponic solution.

[0090] S8. During the process of controlling the motor 210 to drive the toothed plate 250 to move laterally, the toothed plate 250 will mesh with the external gear 451, driving the rotating shaft 452 to rotate, causing the stirring blade 453 and the connecting rod 454 between them to rotate inside the ryegrass hydroponic solution. At the same time, through the round rod 455, the stirring ring 456 is driven to disturb the solution up and down, thereby breaking up particle agglomeration, enhancing mass transfer, and improving the benzothiazole capture efficiency.

[0091] S9. After that, the toothed plate 250 rises and falls again, and the ryegrass hydroponic solution flows through the solid packing material. The benzothiazole compounds inside are selectively adsorbed on the solid packing material, thus completing the sample loading operation.

[0092] S10. Next, the hydrophilic interfering substances on the solid packing are first eluted with a weak solvent, and then the benzothiazole compound is desorbed from the solid packing with a strong solvent.

[0093] S11. Finally, the operator takes the glass container 600 on the far right and puts it into the nitrogen evaporator to enrich the benzothiazole compound, and then stores it.

[0094] The foregoing description of specific exemplary embodiments of the invention is for illustrative and explanatory purposes. These descriptions are not intended to limit the invention to the precise forms disclosed, and it will be apparent that many changes and variations can be made in accordance with the foregoing teachings. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application, thereby enabling those skilled in the art to implement and utilize various different exemplary embodiments of the invention, as well as various different choices and variations. The scope of the invention is intended to be defined by the specification and its equivalents.

Claims

1. An extraction device for benzothiazole compounds in ryegrass hydroponic solution, including a solution treatment chamber and an SPE column; Its features are: It also includes a material conveying mechanism for transporting the SPE column, several liquid storage pipes set above the SPE column, and an extrusion device; The material conveying mechanism includes an electric push rod and a toothed plate at its top. The liquid storage tube includes a fixed tube body, an insert rod located below the fixed tube body, and a bent plate sleeved on the outer end of the insert rod. A stirring part is also provided inside the liquid storage tube located in the middle. A cross tube body is snapped into the bottom end of the fixed tube body. The insert rod slides inside the cross tube body. A through hole is opened inside the rod, and a protruding rod is integrally formed at the end. A sloping groove is opened at the bottom of the bent plate for the protruding rod to slide. The upper end of the bending plate slides and engages with the side of the toothed plate. The electric actuator controls the toothed plate to move the bending plate upward, allowing the protruding rod to move along the inclined groove. The drive rod moves laterally so that the through hole connects with the vertical tube of the cross tube, allowing the solution in the fixed tube to drip into the SPE column. When the toothed plate moves laterally, it can also drive the stirring part to stir the solution in the middle liquid storage tube. The extrusion device includes a pair of racks, an internal gear disposed between the pair of racks, and a piston plate that moves the rear rack. An electric actuator controls the rack plate to move the front rack upward, which in turn drives the meshing internal gear to move the rear rack downward, allowing the piston plate to expel the SPE column solution at a fixed flow rate.

2. The extraction apparatus for benzothiazole compounds in ryegrass hydroponic solution according to claim 1, characterized in that: The culture medium treatment chamber includes a chamber body, a fixed frame welded to the outer wall of the chamber body, a partition plate located below the fixed frame and welded to the chamber body, several limiting frames regularly snapped onto the outer wall of the chamber body, several sealing plugs snapped onto the top surface of the chamber body, and a hanging frame welded to the front wall of the chamber body. Several regularly placed glass containers are also placed on the bottom surface inside the chamber body.

3. The extraction apparatus for benzothiazole compounds in ryegrass hydroponic solution according to claim 2, characterized in that: The interior of the chamber has a through-flow groove, and a placement groove is also provided on the front side of the groove. The partition is fitted with a funnel directly above several glass containers. The positions of several limiting frames correspond to the positions of several squeezing devices. Several sealing plugs are located directly above the liquid storage tube.

4. The extraction apparatus for benzothiazole compounds in ryegrass hydroponic solution according to claim 3, characterized in that: The material conveying mechanism also includes a motor, a lead screw coaxially connected to its output shaft, and a slider sleeved on the outside of the lead screw and slidably connected to the slide groove and the placement groove. The electric push rod is fixedly connected to the top surface of the slider by screws. The outer wall of the toothed plate is provided with a plate surface groove, and the upper end of the bent plate is slidably engaged with the plate surface groove.

5. The extraction apparatus for benzothiazole compounds in ryegrass hydroponic solution according to claim 4, characterized in that: The SPE column includes a column body inserted into the end of the slider plate, a solid packing material placed inside the column body for capturing benzothiazole compounds, and two filter screens snapped into the inside of the column body, with the two filter screens located in the upper and lower directions of the solid packing material.

6. The extraction apparatus for benzothiazole compounds in ryegrass hydroponic solution according to claim 5, characterized in that: The liquid storage tube also includes a first spring attached to the rear end of the insertion rod, the fixed tube body is snapped onto the inner bottom surface of the fixed frame, and the other end of the first spring is attached to the tube wall of the cross tube body.

7. The extraction apparatus for benzothiazole compounds in ryegrass hydroponic solution according to claim 6, characterized in that: The bending plate is slidably connected to the inside of the limiting frame, and the inclined groove is set with the front higher than the back. The size of the top horizontal plate of the bending plate is smaller than the size of the groove on the plate surface.

8. The extraction apparatus for benzothiazole compounds in ryegrass hydroponic solution according to claim 7, characterized in that: The stirring unit includes an external gear, a rotating shaft engaged inside the external gear, and two stirring blades that rotate with the rotating shaft. It also includes a connecting rod connected between the two stirring blades, a round rod rotatably connected to the outside of the connecting rod, and a stirring ring rotatably connected to the top of the round rod. The stirring ring is slidably connected inside the fixed tube, and the external gear meshes with the toothed plate.

9. The extraction apparatus for benzothiazole compounds in ryegrass hydroponic solution according to claim 8, characterized in that: The extrusion device also includes a slide rod that is engaged with the end of the rear rack cross plate and a second spring sleeved on the outside of the slide rod. The two racks are centrally symmetrically distributed. The internal gear meshes with the two racks. The size of the bottom cross plate of the front rack is smaller than the size of the groove on the plate surface. The piston plate is engaged with the bottom end of the slide rod. The elastic force provided by the second spring pushes the rear rack to move upward.

10. The extraction apparatus for benzothiazole compounds in ryegrass hydroponic solution according to any one of claims 1-9, characterized in that, The method for extracting benzothiazole compounds from ryegrass hydroponic solution using the aforementioned apparatus includes the following steps: S1. First, the operator filters the ryegrass hydroponic solution through a filter membrane, adjusts the pH value of the ryegrass hydroponic solution, and adds an internal standard of deuterated benzothiazole to the adjusted ryegrass hydroponic solution to correct the recovery rate. S2. Next, the operator first pours methanol, ultrapure water, treated ryegrass hydroponic solution, weak solvent, and strong solvent into several storage tubes from left to right, and then places the SPE column into the round hole at the end of the slider. S3. Then, start the motor, drive the lead screw to rotate and make the slider move laterally, and make the electric push rod move laterally together with the toothed plate to send the SPE column to the bottom of the storage tube containing methanol. S4. The electric actuator moves the toothed plate upward, and the top horizontal plate of the bent plate located in the groove on the plate surface moves accordingly. This causes the protrusion at the end of the insert rod to move along the inclined groove, moving the insert rod laterally within the cross tube body. After the through hole connects with the vertical tube of the cross tube body, methanol is dripped into the interior of the SPE column. S5. Next, control the electric actuator to reset the toothed plate and bending plate, and start the motor to send the SPE column to the bottom of the leftmost extrusion device. S6. After the electric actuator moves up again, the front rack moves up and drives the rear rack to move down through the meshing internal gear, which in turn drives the slide rod and the piston plate at its bottom to move down, allowing methanol to drip from the SPE column at a fixed flow rate, thereby ensuring the solid packing is wetted, opening the bonding sites, and activating the solid packing. S7. Next, repeat the above operation to allow ultrapure water to flow through the solid packing material, displacing the activation solvent and making the internal environment of the solid packing material consistent with the treated ryegrass hydroponic solution. S8. During the process of controlling the motor to drive the toothed plate to move laterally, the toothed plate will mesh with the external gear, drive the rotating shaft to rotate, and make the stirring blade and the connecting rod between them rotate inside the ryegrass hydroponic solution. At the same time, through the round rod, the stirring ring will be driven to disturb the solution up and down, thereby breaking up particle agglomeration, enhancing mass transfer, and improving the benzothiazole capture efficiency. S9. After that, the toothed plate rises and falls again, and the ryegrass hydroponic solution flows through the solid packing material. The benzothiazole compounds inside are selectively adsorbed onto the solid packing material, thus completing the sample loading operation. S10. Next, the hydrophilic interfering substances on the solid packing are first eluted with a weak solvent, and then the benzothiazole compound is desorbed from the solid packing with a strong solvent. S11. Finally, the operator takes the glass container on the far right and puts it into the nitrogen evaporator to enrich the benzothiazole compound, and then stores it.

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