Amino surface cleaners and sanitizers with fast microbial efficacy

Abstract

Antimicrobial compositions comprising at least an amine based antimicrobial agent and an alkanolamine are disclosed. The disclosed compositions may be free of quaternary ammonium compounds. The disclosed compositions have antimicrobial properties and fast efficacy against yeasts and bacteria.

Description

Cross Reference to Related Applications

[0001] This application claims the benefit of and priority to U.S. Provisional Application No. 63 / 501,214, filed May 10, 2023, which is incorporated by reference herein. BACKGROUND

[0002] Disinfecting and sanitizing surfaces in sensitive areas, such as hospitals, food and beverage facilities, food surfaces, food contact surfaces in restaurants and grocery stores, and clean rooms, is challenging. Antimicrobial compositions used in such applications must achieve a high log reduction of a broad spectrum of microorganisms, including gram-positive and gram-negative bacteria, bacterial spores, yeast, fungi, and viruses. The desired log reduction must be achieved in a short amount of time. Depending on the application, the antimicrobial composition must be able to meet additional criteria, such as leaving no residue, being approved for food contact, or being approved for food surface contact. The present invention arises in this context.

[0003] Summary

[0004] The present disclosure relates to a method of inactivating microorganisms by applying a use composition to a target surface. In some embodiments, the composition consists of a biocidal amine, an alkanolamine, and a non-ionic surfactant. In some embodiments, the composition achieves a 3 log reduction of yeast in 5 minutes at 20°C without mechanical action, and a 4 log reduction of bacteria in 5 minutes at 20°C without mechanical action. In some embodiments, the use composition is free of quaternary ammonium compounds. In some embodiments, the use composition comprises a complexing agent. In some embodiments, the use composition has a pH in the range of about 10 to about 12.

[0005] The present disclosure also relates to a method of inactivating yeast by applying a composition to a target surface, wherein the method achieves a 3 log reduction of yeast in 5 minutes at 20°C. In some embodiments, the composition consists of a biocidal amine, an alkanolamine, and a non-ionic surfactant. In some embodiments, the method also achieves a 4 log reduction of bacteria in 5 minutes at 20°C. In some embodiments, the use composition is free of quaternary ammonium compounds. In some embodiments, the use composition comprises a complexing agent. In some embodiments, the use composition has a pH in the range of about 10 to about 12.

[0006] The present disclosure also relates to a composition for inactivating yeast by applying the composition to a target surface, wherein the composition achieves a 3 log reduction of yeast in 5 minutes at 20°C. In some embodiments, the composition consists of a biocidal amine, a alkanolamine, and a non-ionic surfactant. In some embodiments, the composition also achieves a 4 log reduction of bacteria in 5 minutes at 20°C. In some embodiments, the use of the composition does not include a quaternary ammonium compound. In some embodiments, the use of the composition includes a complexing agent. In some embodiments, the pH of the use of the composition is in the range of about 10 to about 12. BRIEF DESCRIPTION OF DRAWINGS

[0007] Figure 1 Graphs detailing the results of the cleaning efficacy tests are depicted. DETAILED DESCRIPTION

[0008] As used herein, weight percent (wt.%), "percent by weight," "percent weight," %, and the like are synonyms that refer to the concentration of a substance as the weight of that substance divided by the total weight of the composition and multiplied by 100.

[0009] As used herein, the term "about" to modify the amount of an ingredient in the compositions of the present application or used in the methods of the present application refers to variation in the value that can occur, e.g., through typical measuring and liquid handling procedures used in preparing the use solutions in the real world; through inadvertent error when preparing the compositions or practicing the methods; through differences in the manufacture, source, or purity of the ingredients employed in making the compositions or practicing the methods; and the like. The term about also encompasses variations that occur due to different equilibration conditions for a composition that is produced from a particular initial mixture. Whether modified by the term "about" or not, the claims include equivalent values. The term "about" generally allows for variations of 5% in the recited percentage value. For example, if "about 10%" is used, the term "about" generally allows for variations of about 5% in the recited percentage value. Thus, "about 10%" can encompass + / - 0.5% variation.

[0010] It should be noted that, as used in this specification and the appended claims, the singular forms "a," "an," and "the" include plural referents unless the content clearly dictates otherwise. Thus, for example, reference to a composition containing "a compound" includes a mixture of two or more compounds. It should also be noted that the term "or" is generally employed in its sense including "and / or" unless the content clearly dictates otherwise.

[0011] For the purpose of brevity and clarity, any range of values stated in the present specification envisions all values within the range and is to be construed as supporting the requirement of claim limitations that recite any sub-range of values within the specified range. By way of illustrative example, a disclosure of from 1 to 5 in the present specification is to be interpreted to support a claim reciting any of the following ranges of values: 1-5; 1-4; 1-3; 1-2; 2-5; 2-4; 2-3; 3-5; 3-4; and 4-5.

[0012] As used herein, "cleaning" refers to the removal of organic or inorganic soils. In some examples, "cleaning" can refer to any process that aids in the removal of soils.

[0013] As used herein, the term "disinfecting" refers to the biological action of killing vegetative cells and reducing the population of microorganisms, including most recognized pathogenic microorganisms.

[0014] As used herein, the term "microorganism" refers to any non-cellular or unicellular (including colonial) organism. Microorganisms include all prokaryotes. Microorganisms include bacteria (including cyanobacteria), spores, lichens, fungi, protozoa, virioplankton, viroids, viruses, phages, and some algae. As used herein, the term "microbe" is synonymous with microorganism.

[0015] In the field of utilizing clean rooms, it is important to maintain a controlled environment, limiting or eliminating the growth of microorganisms, such as bacteria, yeast, or fungi. Additionally, it is important that these environments not only be free of microbial contamination, but also free of visible stains or soils. Thus, antimicrobial compounds must also be nearly foam-free when applied, and must leave minimal or no residue. Many commercially available antimicrobial agents rely on the use of quaternary ammonium disinfectants, which have high potency against Gram-positive bacteria, but reduced potency against Gram-negative bacteria, and little impact on yeast and fungi.

[0016] One alternative method of eliminating the growth of microorganisms is the use of amine-based antimicrobial compounds. Previous amine-based antimicrobial compositions have been limited by factors including limited microbial potency spectrum, poor cleaning performance, incompatibility with target surfaces, and / or foaming or residue formation. Additionally, some existing antimicrobial compositions do not achieve a desired 3-log reduction within a target time of 2, 5, or 10 minutes of contact with a target surface.

[0017] Surprisingly, it has been discovered that the disclosed biocidal amine in combination with alkanolamine is effective against yeast in a short period of time. In addition to achieving the desired log reduction against yeast in the desired time, the disclosed formulations are effective against a range of microorganisms including gram-negative and gram-positive bacteria as well as yeast, exhibit minimal foaming and residue formation, and are compatible with a range of surfaces including glass, polymers, PVC, pharmaceutical terrazzo, epoxy, and stainless steel. The compound is also free of quaternary ammonium compounds. The disclosed compound is particularly useful for cleanroom applications with stringent requirements, but is generally useful for antimicrobial applications including healthcare as well as food and beverage applications.

[0018] Active ingredient

[0019] In some embodiments, the disclosed compositions comprise an amine, an alkanolamine, a nonionic surfactant, and water. The disclosed compositions can also comprise additional functional ingredients.

[0020] In some embodiments, the disclosed compositions consist essentially of an amine, an alkanolamine, a nonionic surfactant, and water. These compositions can also comprise additional functional ingredients. In the present disclosure, the phrase "consists essentially of includes those ingredients and lacks any ingredient that materially affects the basic and novel characteristic of the compositions or methods. The phrase "consists essentially of excludes other materials that affect the antimicrobial efficacy of the compositions unless such ingredients have been expressly recited therein after this phrase.

[0021] In some embodiments, the disclosed compositions consist of an amine, an alkanolamine, a nonionic surfactant, and water.

[0022] Biocidal amine

[0023] In some embodiments, the amine present in the composition is a biocidal amine. Exemplary biocidal amines include N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine (e.g., CAS No.: 2372-82-9, commercially available as Lonzabac 12), glucoprotamine, or combinations thereof. In some embodiments, the biocidal amine is present in the concentrate at a concentration of about 2 wt% to about 30 wt%, about 2 wt% to about 20 wt%, about 2 wt% to about 10 wt%, about 5 wt% to about 30 wt%, about 10 wt% to about 30 wt%, or about 20 wt% to about 30 wt%. In some embodiments, the biocidal amine is present in the use solution at a concentration of about 0.01 wt% to about 2 wt%, about 0.01 wt% to about 5 wt%, about 0.01 wt% to about 10 wt%, about 2 wt% to about 10 wt%, or about 5 wt% to about 10 wt%.

[0024] Alkanolamine

[0025] In some embodiments, the composition further comprises a alkanolamine with the purpose of enhancing the performance of the antimicrobial agent against bacteria and especially yeast. Alkanolamines are compounds containing hydroxyl groups and amino groups such as monoethanolamine, diethanolamine, triethanolamine, or dimethylethanolamine. In some embodiments, the alkanolamine is present in the concentrate at a concentration of about 2 wt% to about 30 wt%, about 2 wt% to about 20 wt%, about 2 wt% to about 10 wt%, about 5 wt% to about 30 wt%, about 10 wt% to about 30 wt%, or about 20 wt% to about 30 wt%. In some embodiments, the alkanolamine is present in the use solution at a concentration of about 500 ppm to about 0.01 wt%, about 0.01 wt% to about 2 wt%, about 0.01 wt% to about 5 wt%, about 0.01 wt% to about 10 wt%, about 2 wt% to about 10 wt%, or about 5 wt% to about 10 wt%.

[0026] Non-ionic surfactant

[0027] In some embodiments, the disclosed compositions further comprise a non-ionic surfactant. Exemplary non-ionic surfactants include C 12 to C 14 fatty alcohol surfactants, C 12 to C 14EO / PO copolymer surfactants, EO-terminated surfactants, and alkylpolyglycoside surfactants. Fatty alcohol surfactants are the preferred surfactants for use in the present composition as it allows for suitable foam formation upon initial application, but allows the foam formation to decrease over time, which is necessary for cleanroom environments. In some embodiments, the nonionic surfactant is present in the concentrate at a concentration of about 1 wt% to about 30 wt%, about 1 wt% to about 20 wt%, about 1 wt% to about 10 wt%, about 5 wt% to about 30 wt%, about 10 wt% to about 30 wt%, or about 20 wt% to about 30 wt%. In some embodiments, the nonionic surfactant is present in the use solution at a concentration of about 0.01 wt% to about 2 wt%, about 0.01 wt% to about 5 wt%, about 0.01 wt% to about 10 wt%, about 2 wt% to about 10 wt%, or about 5 wt% to about 10 wt%.

[0028] Complexing agent

[0029] In some embodiments, the disclosed composition further comprises a complexing agent. Exemplary complexing agents include aminocarboxylates, EDTA, NTA, GLDA, MGDA, and mixtures thereof. The preferred complexing agent is methylglycinediacetic acid (MGDA). In some embodiments, the complexing agent is present in the use solution at a concentration of about 1 ppm to about 1000 ppm, about 50 ppm to about 500 ppm, or about 50 ppm to about 150 ppm.

[0030] Water

[0031] The disclosed composition is preferably a liquid, thickened liquid, gel, or foam, and comprises water. In some embodiments, the water is present in an amount of about 75 wt% to about 99.9 wt%, about 85 wt% to about 99.9 wt%, about 95 wt% to about 99.9 wt%, or about 99.0 wt% to about 99.9 wt%. In some embodiments, the present composition is provided as a concentrate product intended to be diluted prior to use. In some embodiments, the present composition is provided as a ready-to-use composition.

[0032] In some embodiments, the water is purified, such as water for injection (WFI). The water can also be deionized, distilled, sterile, or bacteriostatic. However, in some embodiments, the water used in the disclosed compositions is not purified or sterilized. In such examples, the water can be non-sterile, such as hard water or tap water, which includes minerals such as calcium, sodium, magnesium, fluoride, copper, iron, chloride, and other minerals. For non-sterile water sources, a chelating agent can be added to complex to the minerals and act as a scale inhibitor. In examples, the chelating agent can be phosphorus-based, aminocarboxylate-based, or other forms of chelating agents. Preferably, the chelating agent is a phosphorus-based chelating agent, such as a phosphonate.

[0033] In some examples, the phosphorus-based chelating agent is a phosphonate. The phosphonate can be added to the concentrate in an amount of about 0.01 wt% to about 2 wt%, about 0.01 wt% to about 1 wt%, about 0.01 wt% to about 0.75 wt%, about 0.01 wt% to about 0.5 wt%, about 0.01 wt% to about 0.1 wt%, about 0.1 wt% to about 2 wt%, about 0.5 wt% to about 2 wt%, about 0.75 wt% to about 2 wt%, or about 1 wt% to about 2 wt%.

[0034] Additional functional ingredient

[0035] In some embodiments, the antimicrobial composition can optionally include additional functional ingredients. Exemplary additional functional ingredients include thickening or gelling agents, co-solvents, additional foaming agents, chelating agents, or mixtures thereof.

[0036] Thickener or gelling agent

[0037] In some applications, it can be desirable for the present compositions to have a greater viscosity or to take the form of a thickened liquid or gel. Exemplary thickening agents include those that do not leave a contaminating residue on the surface of application. Exemplary thickening agents include natural gums such as xanthan gum. Cellulose polymers such as carboxymethyl cellulose can also be used in the present invention. Generally, the concentration of thickening agent used in the present invention will be determined by the desired viscosity within the final composition.

[0038] In some embodiments, the viscosity of the composition is similar to water or near 0 centipoise. In some embodiments, the composition can be a concentrate composition or a ready-to-use composition having a viscosity of 0 centipoise to about 50 centipoise, 0 centipoise to about 100 centipoise, 0 centipoise to about 1000 centipoise, about 50 centipoise to about 750 centipoise, or about 100 centipoise to about 500 centipoise, as determined by a Brookfield viscometer with a number 61 rotor at 20 °C at 100 rpm.

[0039] Cosolvent

[0040] In some embodiments, the composition can include a co-solvent. Preferred co-solvents include glycols and C1-C4 alcohols, such as butylene glycol, propanol, ethanol, methanol, or propylene glycol, among others.

[0041] Additional surfactant or foaming agent

[0042] While the present disclosure emphasizes the need for minimal foaming to reduce undesirable residue in cleanroom applications, in other applications, additional foaming can be a desirable characteristic. The formation of foam can increase the contact time on non-horizontal surfaces, allowing additional contact time for the active ingredients with the target microorganisms to achieve the desired log reduction.

[0043] pH

[0044] In some embodiments, the disclosed composition has a pH in the range of about 7 to about 14, about 8 to about 13, about 9 to about 12, or about 10 to about 12.

[0045] Absence of quaternary ammonium compound

[0046] In some embodiments, the disclosed composition does not contain a quaternary ammonium compound. Quaternary ammonium compounds are commonly used antimicrobial agents in cleaning solutions; however, some potential users prefer alternative antimicrobial agents, such as the disclosed composition. The reluctance to use quaternary ammonium compounds can be due to high manufacturing costs and low efficacy against microorganisms other than gram-positive bacteria.

[0047] Method of inactivating microorganisms

[0048] The present disclosure relates to methods of inactivating microorganisms, particularly bacteria and yeast, in a short amount of time using the disclosed antimicrobial compositions. In some embodiments, the disclosed antimicrobial compositions have a log reduction of greater than 4 logs of bacteria in 2 minutes of contact, a log reduction of greater than 5 logs of bacteria in 2 minutes of contact, a log reduction of 4 logs to 6 logs of bacteria in 2 minutes of contact, a log reduction of 4 logs to 7 logs of bacteria in 2 minutes of contact, a log reduction of 5.5 logs to 6.5 logs of bacteria in 2 minutes of contact, or a log reduction of 6 logs to 6.5 logs of bacteria in 2 minutes of contact at room temperature and without mechanical action. Exemplary test organisms include S. aureus, E. hirae, E. coli, and P. aeruginosa according to EN 13697.

[0049] In some embodiments, the disclosed antimicrobial composition has a 3 log or greater log reduction of yeast within 2 minutes of contact, a 2 log to 5 log log reduction of yeast within 2 minutes of contact, a 3 log to 4 log log reduction of yeast within 2 minutes of contact, or a 3.5 log to 4 log log reduction of yeast within 2 minutes of contact on a surface at room temperature without mechanical action according to EN 13697.

[0050] In some embodiments, the disclosed antimicrobial composition has a greater than 3 log log reduction of fungi within 5 minutes of contact on a surface without mechanical action, a greater than 3.5 log log reduction of fungi within 5 minutes of contact, a greater than 4 log log reduction of fungi within 5 minutes of contact at room temperature, a 3 log to 5 log log reduction of fungi within 5 minutes of contact at room temperature according to EN 13697.

[0051] Microbial strain

[0052] A variety of microorganisms were used in the testing to confirm the efficacy of the disclosed compositions. Among these microorganisms, both gram-positive and gram-negative bacterial strains were tested. The gram-positive bacterial strains used included Enterococcus hirae and Staphylococcus aureus. The gram-negative strains used included Escherichia coli and Pseudomonas aeruginosa. The yeast strain Candida albicans was also tested, as was the fungal strain Aspergillus brasiliensis. These strains are a sampling of potential species that can utilize the current compositions, and in no way encompass the antimicrobial efficacy of the current compositions. The test used was EN 13697.

[0053] Application

[0054] In some embodiments, the disclosed compositions are applied to hard surfaces located in research areas, manufacturing facilities, laboratories, clean rooms, hospitals, resorts, hotels and motels, commercial real estate, schools, and food or beverage manufacturers, among others. Amine-based antimicrobial agents are ideal for hard surfaces that are susceptible to microbial contamination or growth but do not have high soil content, as amines tend to break down and lose functionality upon exposure to soil. Thus, the present compositions are advantageous for use in clean rooms, pharmaceutical manufacturing facilities, laboratories, schools, resorts, hotels and motels, commercial real estate, hospitals, and other areas with minimal soil. Exemplary hard surfaces include counters, sinks, walls, drains, floors, ceilings, tables, carts, benches, transfer hatches, transfer pods, storage areas, cutting boards, knives, laboratory equipment, laboratory instruments, tanks, pipes, evaporators, membranes, heat exchangers, spray heads, nozzles, controls, tools, handles, remote controls, toilet handles, toilet seat covers, bedpan flushers, faucet handles, door knobs or handles, push plates, grab plates, toilet area grab bars, phone receivers, call buttons, tables, window curtains, restricted access barrier systems (RABS), clean room equipment garments, personal protective equipment, laboratory hoods, safety cabinets, laminar flow cabinets, clean room isolators, chair surfaces, chair arms, bed rails, drapes, room light switches, computer mice, computer keyboards, keyboard wrist rests, soap dispensers, and the like.

[0055] Example

[0056] Example 1

[0057] Example 1 tested various formula products for efficacy against various microorganisms, including bacteria and yeast. During the test, various undiluted formula products were contacted with multiple bacterial strains and the yeast strain Candida albicans under clean conditions at room temperature for a period of 5 minutes or 10 minutes according to test procedure EN 13697. To be considered having bactericidal activity, a formula product was required to exhibit at least a 4 log reduction after 5 minutes of exposure, up to 60 minutes of exposure. To be considered having a yeast killing activity, a formula product was required to exhibit at least a 3 log reduction after five minutes of exposure, up to 60 minutes of exposure. The test was performed on experimental formula products 1 listed in Table 1 and compared to a commercially available product, Klercide Low Residual Quaternary Ammonium Salt, from Ecolab. The results are shown in Table 1.

[0058] Experimental formulation 1

[0059]

[0060] Table 1

[0061]

[0062] The data shown in Table 1 indicate that experimental Formula Product 1 was able to achieve the desired 4 log reduction against bacteria after 5 minutes of exposure and the desired 3 log reduction against yeast after five minutes of contact.

[0063] Example 2

[0064] Example 2 tested the foaming performance of experimental Formula Product 1 over time. For this test, 100 ml of experimental Formula Product 1 was placed in a 250 ml graduated cylinder and shaken evenly and rhythmically by hand for 20 times. The height of the foam produced was measured on the graduated cylinder immediately after shaking the graduated cylinder, 1 minute after shaking the graduated cylinder, and five minutes after shaking the graduated cylinder. The results of this experiment are shown in Table 2.

[0065] Table 2

[0066]

[0067] The results shown in Table 2 indicate that some foam formation occurred immediately after application. These results also indicate that the foam decreased rapidly over time. These results are desirable for cleanroom applications because some foam formation helps to support cleaning, but excess foam formation can lead to residue or smudging.

[0068] Example 3

[0069] Example 3 tested the residue characteristics of various formula products on stainless steel surfaces as a result of spraying and wiping. When experimental Formula Product was sprayed on stainless steel and allowed to evaporate, a slight residue was left behind.

[0070]

[0071] When sprayed on glass, or when wiped on glass or stainless steel and allowed to evaporate, no residue was left behind.

[0072] Example 4

[0073] Example 4 tested the cleaning characteristics of various formula products. Along with experimental Formula Product 1, two commercial formula products were tested, Klercide Neutral Detergent and Anios Clean Surf, both of which are commercially available from Articon Group. These formula products were tested with tap water as a control.

[0074] The test was done using the Gardner Rub Test (Gardner device model 494; DIN-ASTM-515; Erichsen GmbH & Co. KG). For the test, white PVC test coupons were coated with 2 grams of soil (IPP 83 / 21, available from WFK in Krefeld, Germany). 12.5 ml of test detergent or tap water control was applied to the test strip, and another 12.5 ml of test detergent or control was added to the sponge on the device, for a total of 25 ml per test cycle. The test strip was then rubbed back and forth over the surface of the coated test strip using a polyester flat sponge for 100 cycles. After the test was complete, the test strips were removed from the machine, rinsed, dried, and their degree of cleaning was evaluated and judged by measuring the whiteness of the test strips. The whiteness of the test coupons was measured with a Minolta colorimeter CR-200, and the percentage of soil removed was recorded. If the test coupon was completely clean (all soil was removed), the test had a measurement of 100%. Seven replicate tests were done for each cleaning solution. The results of the experiment are shown in the percent cleaning in Tables 3 and Figure 1

[0075] Table 3

[0076]

[0077]

[0078] The results shown in Tables 3 and Figure 1 show that the average cleaning efficacy of the experimental formula products was 85.55%. The highest average efficacy was observed with the commercial formula product Klercide Neutral Detergent, which had an average efficacy of 87.48%. The lowest average efficacy of the Anios cleaning surfactant was 68.10%, while the average cleaning efficacy of the control was 64.32%. This data indicates that the disclosed compositions have higher or comparable cleaning efficacy than other commercially available cleaning products.

[0079] Example 5

[0080] Examples 1-4 tested the performance of experimental formula product 1 under low soiling conditions. Example 5 compared the performance of experimental formula product 1 under low soiling conditions and high soiling conditions.

[0081] ​Table 4 shows the results of testing experimental formula product 1 under low and high soiling conditions. A total of eighteen tests were conducted. Experimental formula product 1 was evaluated as a bactericide, a yeasticide, and a virucide against enveloped viruses under low soiling conditions. Experimental formula product 1 was tested in each of these three functions in suspension tests, surface tests without mechanical action, and surface tests with mechanical action. For each of these variables, the contact time was measured in minutes. The time period in Table 4 represents the number of minutes that experimental formula product 1 was required to satisfactorily function as a bactericide, a yeasticide, and a virucide according to the requirements of each of the three tests. The same procedure was conducted under high soiling conditions.

[0082] Table 4

[0083]

[0084] The results in Table 4 show that experimental formula product 1 provides substantially the same cleaning under low and high soiling conditions. For example, experimental formula product 1 adequately functions as a bactericide under low and high soiling conditions for the same time period. This is advantageous because experimental formula product 1 can be used as a bactericide, a yeasticide, and a virucide in both low and high soiling applications, which increases the applicability of the formula product.

[0085] The above specification, examples and data provide a complete description of the manufacture and use of the composition of the application. Since many embodiments of the application can be made without departing from the spirit and scope of the application, the application resides in the claims hereinafter appended.

Claims

1. A method of inactivating microorganisms, the method comprising applying to a surface a use composition comprising: a. about 0.01 wt% to about 2 wt% of a biocidal amine; b. about 0.01 wt% to about 2 wt% of an alkanolamine; and c. about 0.01 wt% to about 2 wt% of a non-ionic surfactant.

2. The method of claim 1, wherein the use composition achieves a 3 log reduction of Candida albicans in 5 minutes at 20°C without mechanical action.

3. The method of claim 1 or 2, wherein the use composition achieves a 4 log reduction of Enterococcus hirae in 5 minutes at 20°C without mechanical agitation.

4. The method of any one of claims 1 to 3, wherein the use composition is free of quaternary ammonium compounds.

5. The method of any one of claims 1 to 4, wherein the biocidal amine is selected from the group consisting of N-(3-aminopropyl)-N-dodecylpropane-1,3-diamine or glycinin.

6. The method of any one of claims 1 to 5, wherein the alkanolamine is selected from the group consisting of monoethanolamine, diethanolamine, triethanolamine, dimethylethanolamine, and combinations thereof.

7. The method of any one of claims 1 to 6, wherein the non-ionic surfactant is selected from the group consisting of an oxirane / propylene oxide copolymer, an oxirane-terminated surfactant, an alkylpolyglycoside, and mixtures thereof.

8. The method of any one of claims 1 to 7, wherein the non-ionic surfactant is a C 12 -C 14 EO / PO surfactant.

9. The method of any one of claims 1 to 8, the composition further comprising a complexing agent.

10. The method of claim 9, wherein the complexing agent is selected from the group consisting of an aminocarboxylate, EDTA, NTA, GLDA, MGDA, and mixtures thereof.

11. The method of any one of claims 1 to 10, the use composition further comprising a C1-C4 alcohol.

12. The method of any one of claims 1 to 11, wherein the use composition has a pH of about 10 to about 12.

13. The method of any one of claims 1 to 12, further comprising diluting a concentrate composition with water to form the use composition.

14. The method of any one of claims 1 to 13, wherein the concentrate composition comprises: a. about 2 wt% to about 20 wt% of a biocidal amine; b. about 2 wt% to about 20 wt% of an alkanolamine; and c. about 1 wt% to about 10 wt% of a non-ionic surfactant.

15. A method of inactivating yeast, the method comprising applying to a surface a composition comprising: a. about 0.01 wt% to about 2 wt% of a biocidal amine; b. about 0.01 wt% to about 2 wt% of an alkanolamine; and c. about 0.01 wt% to about 2 wt% of a non-ionic surfactant, wherein the composition achieves a 3 log reduction of Candida albicans in 5 minutes at 20°C.

16. The method of claim 15, wherein the use composition achieves a 4 log reduction of Enterococcus hirae in 5 minutes at 20°C.

17. The method of claim 15 or 16, wherein the use composition is free of quaternary ammonium compounds.

18. The method of any one of claims 15 to 17, wherein the biocidal amine is selected from the group consisting of N-(3-aminopropyl)-N-dodecylpropane-l,3-diamine, glycinin, or combinations thereof.

19. The method of any one of claims 15 to 18, wherein the alkanolamine is selected from the group consisting of monoethanolamine, diethanolamine, triethanolamine, dimethylethanolamine, and combinations thereof.

20. The method of any one of claims 15 to 19, wherein the non-ionic surfactant is selected from the group consisting of an oxirane / propylene oxide copolymer, an oxirane-terminated surfactant, an alkylpolyglycoside, and mixtures thereof.

21. The method of any one of claims 15-20, wherein the non-ionic surfactant is C 12 -C 14 EO / PO surfactant.

22. The method of any one of claims 15 to 21, the composition further comprising a complexing agent.

23. The method of claim 22, wherein the complexing agent is selected from the group consisting of an aminocarboxylate, EDTA, NTA, GLDA, MGDA, and mixtures thereof.

24. The method of any one of claims 15 to 23, the use composition further comprising a Ci to C4 alcohol.

25. The method of any one of claims 15 to 24, wherein the use composition has a pH of about 10 to about 12.

26. The method of any one of claims 15 to 25, further comprising diluting a concentrated composition with water to form the use composition.

27. The method of claim 26, wherein the concentrated composition comprises: a. about 2 wt% to about 20 wt% of a biocidal amine; b. about 2 wt% to about 20 wt% of an alkanolamine; and c. about 1 wt% to about 10 wt% of a non-ionic surfactant.

28. A composition for inactivating yeast, the composition comprising: a. about 0.01 wt% to about 2 wt% of a biocidal amine; b. about 0.01 wt% to about 2 wt% of an alkanolamine; and c. about 0.01 wt% to about 2 wt% of a non-ionic surfactant, wherein the composition achieves a 3 log reduction of Candida albicans in 5 minutes at 20°C.

29. The composition of claim 28, wherein the composition achieves a 4 log reduction of Enterococcus hirae in 5 minutes at 20°C.

30. The composition of claim 28 or 29, wherein the use composition is free of quaternary ammonium compounds.

31. The composition of any one of claims 28 to 30, wherein the biocidal amine is selected from the group consisting of N-(3-aminopropyl)-N-dodecylpropane-l,3-diamine, glycinin, and combinations thereof.

32. The composition of any one of claims 28-31, wherein the alkanolamine is selected from the group consisting of monoethanolamine, diethanolamine, triethanolamine, dimethylethanolamine, and combinations thereof.

33. The composition of any one of claims 28-32, wherein the nonionic surfactant is selected from the group consisting of an oxirane / propylene oxide copolymer, an oxirane-terminated surfactant, an alkylpolyglycoside, and mixtures thereof.

34. The composition of any one of claims 28-33, wherein the non-ionic surfactant is C 12 -C 14 EO / PO surfactant.

35. The composition of any one of claims 28-34, further comprising a complexing agent.

36. The composition of claim 35, wherein the complexing agent is selected from the group consisting of aminocarboxylate, EDTA, NTA, GLDA, MGDA, and mixtures thereof.

37. The composition of any one of claims 28-36, further comprising a Ci to C4 alcohol.

38. The composition of any one of claims 28-37, wherein the pH of the composition is from about 10 to about 12.

Images