Vitamin B12 feed additive and preparation method thereof

Abstract

The invention relates to a vitamin B12 feed additive, which comprises a vitamin B12 concentrated solution, an inclusion agent and a diluent, and also provides a preparation method of the feed additive, the vitamin B12 is subjected to inclusion protection by adopting the inclusion agent, and then the vitamin B12 is uniformly mixed and granulated by combining with the diluent to obtain the vitamin B12 feed additive. A vitamin B12 inclusion technology and a uniform mixing and granulating technology are adopted, so that the quality stability of the vitamin B12 feed additive is remarkably improved, and the utilization rate of the vitamin B12 is effectively increased.

Description

Technical Field

[0001] This invention belongs to the field of feed additives, specifically relating to a vitamin B12 feed additive and its preparation method. Background Technology

[0002] Vitamin B12, abbreviated as VB12, generally refers specifically to cyanocobalamin. It is an essential coenzyme in the synthesis of ribonucleic acid and deoxyribonucleic acid. Vitamin B12 deficiency in the body can cause nutritional deficiency diseases characterized by pernicious anemia, as well as pathological changes in the peripheral nervous system.

[0003] Vitamin B12 participates in the metabolic activities of various substances in livestock, poultry, and fish as a coenzyme. It participates in the synthesis of nucleic acids and proteins in livestock, poultry, and fish, promoting cell development and maturation, and accelerating weight gain. When vitamin B12 is deficient, the utilization of sugar in the tissues of livestock, poultry, and fish is impaired, resulting in decreased appetite, slow growth or weight loss, severe emaciation, pica, and death from extreme anemia. Vitamin B12 promotes growth and development and improves the utilization rate of feed protein. Plants do not contain vitamin B12 and cannot produce it; therefore, animals whose primary food source is forage have a greater need for vitamin B12 supplementation. Since the "mad cow disease" outbreak in Europe, the use of vitamins and other nutritional fortifiers with well-defined chemical structures to replace meat and bone meal has seen greater development potential. Currently, most of the vitamin B12 produced worldwide is used as a feed additive in the feed industry.

[0004] Vitamin B12 is easily destroyed by strong light or ultraviolet radiation, resulting in a decrease in its content and affecting the quality stability of vitamin B12 feed additives. Moreover, uneven mixing leads to low content uniformity, which greatly reduces the utilization rate of vitamin B12.

[0005] Therefore, it is necessary to develop a B12 vitamin feed additive with good stability and high content uniformity. Summary of the Invention

[0006] This invention provides a vitamin B12 feed additive and its preparation method. By first encapsulating and protecting vitamin B12 with an inclusion agent, and then mixing and granulating it with a diluent, the quality stability and content uniformity of the vitamin B12 feed additive are significantly improved, effectively increasing the utilization rate of vitamin B12.

[0007] To achieve the objectives of this invention, the first aspect of this invention relates to a vitamin B12 feed additive, comprising: vitamin B12 concentrate, an encapsulating agent, and a diluent.

[0008] The vitamin B12 concentrate (calculated as vitamin B12) and the inclusion agent have a weight percentage of 1:1.5 to 3.0.

[0009] Preferably, the vitamin B12 concentrate (calculated as vitamin B12) and the inclusion agent are in a weight percentage ratio of 1:2.

[0010] The vitamin B12 concentrate (calculated as vitamin B12) and diluent are present in a weight ratio of 1:70-84.

[0011] Preferably, the vitamin B12 concentrate (calculated as vitamin B12) and diluent are in a weight ratio of 1:80.

[0012] The inclusion agent is selected from at least one of sodium carboxymethyl cellulose, hydroxypropyl methyl cellulose, and hydroxyethyl cellulose;

[0013] Preferably, the inclusion agent is sodium carboxymethyl cellulose.

[0014] The diluent is selected from at least one of corn starch, dicalcium phosphate, calcium carbonate, and mannitol.

[0015] Preferably, the diluent is corn starch.

[0016] The vitamin B12 concentrate has a unit of 16,000 to 22,000 ug / ml.

[0017] The second aspect of this invention relates to a method for preparing the feed additive described in the first aspect, comprising the following steps:

[0018] (1) The vitamin B12 concentrate was mixed with an inclusion agent and spray-dried to obtain vitamin B12 inclusion powder;

[0019] (2) Mix the vitamin B12 inclusion complex powder with the diluent and sieve to obtain vitamin B12 feed additive.

[0020] The moisture content of the vitamin B12 inclusion complex powder is controlled to be below 3%.

[0021] The sieve mesh size is 60-80 mesh.

[0022] Preferably, the sieve mesh size is 60 mesh.

[0023] The technical solution of the present invention has at least the following beneficial technical effects:

[0024] 1. The present invention uses an encapsulating agent with strong adhesion and light stability to encapsulate vitamin B12, making it resistant to light oxidation and improving the quality stability of vitamin B12.

[0025] 2. This invention uses a dispersant for mixing and granulation, which significantly improves the uniformity of vitamin B12 feed additive content.

[0026] 3. This invention uses an encapsulating agent to first encapsulate and protect vitamin B12, and then mixes it evenly with a diluent and granulates it. As a result, the quality stability and content uniformity of vitamin B12 feed additives are significantly improved. The quality stability of vitamin B12 feed additives is improved by 76.6%, and the content uniformity meets the standards, effectively improving the utilization rate of vitamin B12. Detailed Implementation

[0027] The invention is illustrated below with examples. It should be understood that these examples are for illustrative purposes only and not for limiting the invention. The scope and core content of the invention are defined by the claims.

[0028] The vitamin B12 concentrate used in the following examples was obtained by extracting and purifying the fermentation broth of denitrifying Pseudomonas aeruginosa with resin to obtain a liquid containing adenosylcobalamin, followed by cyanidation conversion to obtain a liquid containing vitamin B12 (cyanocobalamin), and finally, through purification and concentration processes, a vitamin B12 concentrate (potency 16000-22000 ug / ml) was obtained.

[0029] Quality control of vitamin B12 feed additives: particle size (60 mesh sieve) ≥ 98.0%, vitamin B12 content < 1.3% for 1.0% < 1.3%, and moisture content < 8.0% for 3.0% < 8.0%.

[0030] Accelerated destructive testing conditions: temperature 40℃, relative humidity 70-75%, observation period 6 months.

[0031] The content uniformity test method in the following examples was determined according to the method in "Feed Additive Vitamin B12 (Cyanocobalamin) Powder GB / T9841-2006".

[0032] Example 1

[0033] Prepare a 2 cubic meter mixing tank, add 0.7 cubic meters of vitamin B12 concentrate (unit 16000ug / ml), which weighs 11.2 kg, and add 16.8 kg of sodium carboxymethyl cellulose while stirring. Stir until uniform, and spray dry to obtain vitamin B12 inclusion complex powder with a weight of 28.6 kg.

[0034] Prepare a 2 cubic meter cone mixer, add the above-mentioned inclusion compound powder, then add 28 kg of calcium carbonate and mix until homogeneous. Repeat this process, adding the remaining calcium carbonate in proportion equal to the weight of the material already in the mixer, until the total calcium carbonate content reaches 935 kg. Mix until homogeneous, and sieve (60 mesh) to obtain 1000 kg of vitamin B12 feed additive with a vitamin B12 content of 1.11% (g / g) and a moisture content of 3.6% (g / g). Accelerated test results (6 months): vitamin B12 content 1.09% (g / g).

[0035] Example 2

[0036] Prepare a 2 cubic meter mixing tank, add 0.7 cubic meters of vitamin B12 concentrate (unit 18000ug / ml), which weighs 12.6 kg. While stirring, add 25.2 kg of sodium carboxymethyl cellulose and stir until uniform. Spray dry to obtain vitamin B12 inclusion complex powder, weighing 38.1 kg.

[0037] Prepare a 2 cubic meter cone mixer, add the above-mentioned inclusion compound powder, then add 38 kg of corn starch and mix until homogeneous. Repeat this process, adding the remaining corn starch in proportion to the existing material in the mixer, until the total corn starch volume reaches 920 kg. Mix until homogeneous, then sieve (60 mesh) to obtain 1000 kg of vitamin B12 feed additive with a vitamin B12 content of 1.16% (g / g) and a moisture content of 4.2% (g / g). Accelerated test results (6 months): Vitamin B12 content 1.16% (g / g).

[0038] Example 3

[0039] Prepare a 2 cubic meter mixing tank, add 0.6 cubic meters of vitamin B12 concentrate (unit 20000ug / ml), which is equivalent to 12.0 kg in weight, and add 30 kg of hydroxypropyl methylcellulose while stirring. Stir until uniform, and spray dry to obtain vitamin B12 inclusion complex powder with a weight of 43.2 kg.

[0040] Prepare a 2 cubic meter cone mixer, add the above-mentioned inclusion compound powder, then add 43 kg of dicalcium phosphate and mix until homogeneous. Repeat this process, adding the remaining dicalcium phosphate in proportion to the existing material in the mixer, until the total dicalcium phosphate content reaches 900 kg. Mix until homogeneous, and sieve (60 mesh) to obtain 1000 kg of vitamin B12 feed additive with a vitamin B12 content of 1.12% (g / g) and a moisture content of 5.9% (g / g). Accelerated test results (6 months): vitamin B12 content 1.11% (g / g).

[0041] Example 4

[0042] Prepare a 2 cubic meter mixing tank, add 0.6 cubic meters of vitamin B12 concentrate (unit 22000ug / ml), which is equivalent to 13.2 kg in weight, and add 39.6 kg of hydroxyethyl cellulose while stirring. Stir until uniform, and spray dry to obtain vitamin B12 inclusion complex powder with a weight of 53.9 kg.

[0043] Prepare a 2 cubic meter cone mixer, add the above-mentioned inclusion complex powder, then add 54 kg of mannitol and mix until homogeneous. Repeat this process, adding the remaining mannitol in proportion to the existing material in the mixer, until the total mannitol volume reaches 950 kg. Mix until homogeneous, and sieve (60 mesh) to obtain 1050 kg of vitamin B12 feed additive with a vitamin B12 content of 1.13% (g / g) and a moisture content of 4.3% (g / g). Accelerated test results (6 months): vitamin B12 content 1.11% (g / g).

[0044] Example 5

[0045] Prepare a 2 cubic meter mixing tank, add 0.7 cubic meters of vitamin B12 concentrate (unit 19000ug / ml), which is equivalent to 13.3 kg in weight, and add 30 kg of hydroxyethyl cellulose while stirring. Stir until uniform, and spray dry to obtain vitamin B12 inclusion complex powder with a weight of 44.6 kg.

[0046] Prepare a 2 cubic meter cone mixer, add the above-mentioned inclusion compound powder, then add 45 kg of calcium carbonate and mix until homogeneous. Repeat this process, adding the remaining calcium carbonate in proportion equal to the weight of the material already in the mixer, until the total calcium carbonate content reaches 925 kg. Mix until homogeneous, and sieve (60 mesh) to obtain 1050 kg of vitamin B12 feed additive with a vitamin B12 content of 1.26% (g / g) and a moisture content of 7.7% (g / g). Accelerated testing showed a vitamin B12 content of 1.22% (g / g).

[0047] Comparison Case 1

[0048] Prepare a 2 cubic meter mixing tank, add 0.7 cubic meters of concentrated vitamin B12 solution (unit 18000ug / ml), equivalent to 12.6 kg, and stir. Add 950 kg of corn starch and stir until homogeneous. Spray dry and sieve (60 mesh screen) to obtain 1000 kg of vitamin B12 feed additive with a vitamin B12 content of 1.20% (g / g) and a moisture content of 3.8% (g / g). Accelerated testing showed a vitamin B12 content of 1.14% (g / g).

[0049] Table 1. Comparison of accelerated stability test results of vitamin B12 content in vitamin B12 feed additives:

[0050]

[0051]

[0052] *Stability of test sample content compared to control content (%) = (Change rate of control content - Change rate of test sample content) * 100 / Change rate of control sample content

[0053] Table 2 Comparison of Content Uniformity Test Results

[0054]

Claims

1. A vitamin B12 feed additive, comprising: The vitamin B12 concentrate, inclusion agent, and diluent are characterized in that the weight percentage of the vitamin B12 concentrate (calculated as vitamin B12) and the inclusion agent is 1:1.5 to 3.0, and the weight percentage of the vitamin B12 concentrate (calculated as vitamin B12) and the diluent is 1:70 to 84.

2. The vitamin B12 feed additive according to claim 1, characterized in that... The inclusion agent is selected from at least one of sodium carboxymethyl cellulose, hydroxypropyl methyl cellulose, and hydroxyethyl cellulose.

3. The vitamin B12 feed additive according to claim 1, characterized in that... The diluent is selected from at least one of corn starch, dicalcium phosphate, calcium carbonate, and mannitol.

4. The vitamin B12 feed additive according to claim 1, characterized in that... The vitamin B12 concentrate (calculated as vitamin B12) and the inclusion agent are in a weight ratio of 1:

2.

5. The vitamin B12 feed additive according to claim 1, characterized in that... The vitamin B12 concentrate (calculated as vitamin B12) and diluent are in a weight ratio of 1:

80.

6. The vitamin B12 feed additive according to claim 1, characterized in that... The vitamin B12 concentrate has a unit of 16,000 to 22,000 ug / ml.

7. The method for preparing the feed additive according to any one of claims 1-6, characterized in that... Includes the following steps: (1) The vitamin B12 concentrate was mixed with an inclusion agent and spray-dried to obtain vitamin B12 inclusion powder; (2) Mix the vitamin B12 inclusion complex powder with the diluent and sieve to obtain vitamin B12 feed additive.

8. The method for preparing the feed additive as described in claim 7, characterized in that... The moisture content of the vitamin B12 inclusion complex powder is controlled to be below 3%.

9. The method for preparing the feed additive as described in claim 7, characterized in that... The sieve mesh size is 60-80 mesh.

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