Sample analyzer, kit, and control method for sample analyzer

Abstract

The embodiment of the invention provides a sample analyzer, a kit and a control method of the sample analyzer. The sample analyzer comprises a kit storage area, a sample dispensing mechanism, a driving mechanism, a detection mechanism and a controller, the controller is used for controlling the driving mechanism to place the capturing head in a sample in the sample accommodating part so as to enable the capturing head to capture a target substance in the sample; controlling the driving mechanism to take out the capturing head from the sample, and controlling the driving mechanism to place the capturing head in the reagent in the reagent accommodating part for reaction to obtain a to-be-detected product; controlling a detection mechanism to detect the to-be-detected product so as to obtain a detection result of the sample; the controller is also used for controlling the driving mechanism to drive the capturing head to rotate after the capturing head is taken out of the sample and / or the reagent. Interference substances on the capture head are removed by driving the capture head to rotate, interference of the interference substances on detection of a to-be-detected product is reduced, and the accuracy of sample detection is improved.

Description

Sample analyzer, reagent kit, and control method for sample analyzer Technical Field

[0001] The present application relates to the technical field of medical equipment, and in particular to a sample analyzer, a reagent kit, and a control method for the sample analyzer. Background Art

[0002] In the field of medical testing, the detection of target substances such as specific proteins, peptides, small molecules, etc. in body fluids, such as blood, can reflect the physiological or pathological state of the human body, and thus provide a basis for clinical diagnosis. It is the foundation of evidence-based medicine.

[0003] The relevant technology can be achieved by immersing a movable probe into the sample, allowing the probe to capture the target substance in the sample, and then immersing the probe into a reagent for reaction to obtain a test liquid; this method usually results in more non-target substances being bound to the probe, and these non-target substances will interfere with the detection results of the target substance in the test liquid.

[0004] Summary of the Invention

[0005] The present application provides a sample analyzer, a reagent kit, and a control method for the sample analyzer, aiming to improve the detection accuracy of the sample analyzer.

[0006] In a first aspect, an embodiment of the present application provides a sample analyzer, comprising:

[0007] a reagent kit storage area, wherein at least one reagent kit can be placed in the reagent kit storage area, wherein the reagent kit comprises a sample receiving portion, a capture head, and a reagent receiving portion, wherein the reagent receiving portion is filled with a reagent;

[0008] a sample dispensing mechanism, the sample dispensing mechanism being used to dispense the sample into the sample containing portion;

[0009] A driving mechanism, the driving mechanism is used to drive the capture head to move;

[0010] A detection mechanism, the detection mechanism is used to detect the product to be tested prepared based on the sample;

[0011] a controller configured to control the driving mechanism to place the capture head into the sample in the sample holding portion so that the capture head captures the target substance in the sample; control the driving mechanism to remove the capture head from the sample, and control the driving mechanism to place the capture head into the reagent in the reagent holding portion for reaction to obtain the product to be tested; and control the detection mechanism to detect the product to be tested to obtain a detection result of the sample;

[0012] The controller is further configured to control the driving mechanism to drive the capture head to rotate after the capture head is removed from the sample and / or the reagent.

[0013] In a second aspect, an embodiment of the present application provides a sample analyzer, comprising:

[0014] a reagent kit storage area, wherein at least one reagent kit can be placed in the reagent kit storage area, wherein the reagent kit comprises a sample receiving portion, a capture head, and a reagent receiving portion, wherein the reagent receiving portion is filled with a reagent;

[0015] a sample dispensing mechanism, the sample dispensing mechanism being used to dispense the sample into the sample containing portion;

[0016] A driving mechanism, the driving mechanism is used to drive the capture head to move;

[0017] A detection mechanism, the detection mechanism is used to detect the product to be tested prepared based on the sample;

[0018] A controller is used to control the driving mechanism to sequentially move the capture head into the sample in the sample holding portion, the reagent in the reagent holding portion, and the detection mechanism, so that the capture head captures the target substance in the sample, the target substance captured by the capture head reacts with the reagent to obtain the product to be tested, and the detection mechanism is controlled to detect the product to be tested, so as to obtain the detection result of the sample in one test.

[0019] In a third aspect, an embodiment of the present application provides a sample analyzer, comprising:

[0020] a reagent kit storage area, wherein at least one reagent kit can be placed in the reagent kit storage area, wherein the reagent kit comprises a sample receiving portion, a capture head, and a reagent receiving portion, wherein the reagent receiving portion is filled with a reagent;

[0021] a sample dispensing mechanism, the sample dispensing mechanism being used to dispense the sample into the sample containing portion;

[0022] A driving mechanism, the driving mechanism is used to drive the capture head to move;

[0023] A detection mechanism, the detection mechanism is used to detect the product to be tested prepared based on the sample;

[0024] a liquid droplet removal mechanism, the liquid droplet removal mechanism being used to remove liquid droplets on the capture head;

[0025] a controller configured to control the driving mechanism to place the capture head into the sample in the sample holding portion so that the capture head captures the target substance in the sample; control the driving mechanism to remove the capture head from the sample, and control the driving mechanism to place the capture head into the reagent in the reagent holding portion for reaction to obtain the product to be tested; and control the detection mechanism to detect the product to be tested to obtain a detection result of the sample;

[0026] The controller is further configured to control the liquid droplet removal mechanism to remove at least part of the liquid droplets on the capture head after the capture head is removed from the sample and / or the reagent.

[0027] In a fourth aspect, an embodiment of the present application provides a reagent kit, wherein the reagent kit is configured to include a sample holding portion having an opening and a reagent holding portion, and the reagent kit is also pre-installed with a capture head;

[0028] The capture head includes a capture portion, the capture portion forms a plurality of surface contact structures, and the surface area of ​​the capture head is 1 to 5 times the surface area of ​​a cylinder of the same length and width;

[0029] The sample holding portion is used to hold a sample and is for the capture head to be placed therein so that the capture portion captures the target substance in the sample;

[0030] The reagent containing portion is filled with a reagent, and is for the capture head to be placed therein so that the target substance captured by the capture head reacts with the reagent to obtain a product to be tested.

[0031] In a fifth aspect, an embodiment of the present application provides a method for controlling a sample analyzer, comprising:

[0032] Controlling the sample dispensing mechanism to dispense the sample into the sample receiving portion of the reagent kit;

[0033] controlling the driving mechanism to place the capture head into the sample in the sample holding portion so that the capture head captures the target substance in the sample;

[0034] controlling the driving mechanism to remove the capture head from the sample;

[0035] Controlling the driving mechanism to place the capture head in the reagent of the reagent containing portion of the reagent kit to react and obtain a product to be tested;

[0036] Controlling the detection mechanism to detect the product to be tested to obtain the test result of the sample to be tested;

[0037] Wherein, after the capture head is removed from the sample and / or the reagent, at least part of the droplets on the capture head are removed.

[0038] In a sixth aspect, an embodiment of the present application provides a sample analyzer, comprising:

[0039] A reagent kit storage area, wherein at least one reagent kit can be placed in the reagent kit storage area, and the reagent kit includes a receiving portion and a capture head;

[0040] a dispensing mechanism for dispensing a sample into the containing portion, the containing portion being pre-filled with a reagent; or the containing portion comprising a sample containing portion and a reagent containing portion, the reagent containing portion being pre-filled with a reagent, the dispensing mechanism for dispensing a sample into the sample containing portion and for dispensing the reagent in the reagent containing portion into the sample containing portion;

[0041] A driving mechanism, the driving mechanism is used to drive the capture head to move;

[0042] A detection mechanism, the detection mechanism is used to detect the product to be tested prepared based on the sample;

[0043] A controller is used to control the driving mechanism to place the capture head in the container including the sample and the reagent so that the capture head captures the target substance in the sample, and to react in the container to obtain the product to be tested; and to control the detection mechanism to detect the product to be tested to obtain the detection result of the sample.

[0044] The sample analyzer, test kit, and control method for the sample analyzer provided in the embodiments of the present application remove interfering substances on the capture head by driving the capture head to rotate after the capture head captures the target substance from the sample and removes it, and / or driving the capture head to rotate after the capture head reacts with the reagent and removes it, thereby reducing the interference of the interfering substances on the detection of the product to be tested and improving the accuracy of sample detection.

[0045] It should be understood that the above general description and the following detailed description are merely exemplary and explanatory and do not limit the disclosure of the embodiments of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0047] FIG1 is a schematic structural diagram of a sample analyzer provided in one embodiment of the present application;

[0048] Figures 2-3 are schematic diagrams of the kit in some embodiments;

[0049] FIG4 is a schematic diagram of a capture head rotating in a sample receiving portion in some embodiments;

[0050] FIG5 is a schematic diagram of a capture head rotating in a reagent holding portion in some embodiments;

[0051] FIG6 is a schematic diagram of a detection mechanism performing detection in some embodiments;

[0052] 7-9C are schematic diagrams of the structures of the capture head in some embodiments;

[0053] FIG10 is a schematic diagram of a coating on a capture head in some embodiments;

[0054] FIG11 is a schematic diagram of a capture head rotating in a cleaning liquid receiving portion in some embodiments;

[0055] Figures 12-14 are schematic diagrams of kits in other embodiments;

[0056] FIG15 is a structural diagram of a sample analyzer provided by another embodiment of the present application;

[0057] FIG16 is a schematic structural diagram of a kit provided in an embodiment of the present application;

[0058] Figure 17 is a schematic diagram of a kit in some other embodiments of the present application;

[0059] FIG18 is a schematic structural diagram of a capture head provided in an embodiment of the present application;

[0060] FIG19 is a flow chart of a control method of a sample analyzer provided in one embodiment of the present application.

[0061] Description of reference numerals:

[0062] 110. Reagent kit storage area; 120. Sample dispensing mechanism; 130. Driving mechanism; 140. Detection mechanism; 150. Controller; 160. Droplet removal mechanism; 10. Reagent kit; 101. Sample holding portion; 102. Reagent holding portion; 101a. Holding portion; 103. Pipette head holding portion; 104. Capture head holding portion; 105. Substrate holding portion; 106. Cleaning liquid holding portion; 107. Droplet holding portion; 1071. Buffer structure; 108. Reserved cavity portion; 11. Capture head; 111. Connecting portion; 112. Capture portion; 1121. Surface contact structure; 112A. Column; 112B. Spherical surface; 112C. Support rod; 1131. Nanosphere; 12. Pipette head. DETAILED DESCRIPTION

[0063] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are part of the embodiments of this application, not all of them. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0064] The flowcharts shown in the accompanying drawings are for illustrative purposes only and do not necessarily include all contents and operations / steps, nor must they be executed in the order described. For example, some operations / steps may be decomposed, combined, or partially merged, so the actual execution order may vary depending on the actual situation.

[0065] The following describes some embodiments of the present application in detail with reference to the accompanying drawings. In the absence of conflict, the following embodiments and features therein may be combined with each other.

[0066] Please refer to FIG1 , which is a schematic structural diagram of a sample analyzer provided in an embodiment of the present application.

[0067] The sample analyzer includes a reagent cartridge storage area 110 , a sample dispensing mechanism 120 , a driving mechanism 130 , a detection mechanism 140 , and a controller 150 .

[0068] The reagent kit storage area 110 can hold at least one reagent kit 10 . The reagent kit 10 includes a sample receiving portion 101 , a capture head 11 , and a reagent receiving portion 102 . The reagent receiving portion 102 contains a reagent.

[0069] In some embodiments, the sample analyzer further includes a reagent kit dispatching mechanism, which can dispatch the reagent kit 10 from the reagent kit storage area 110 to the sample dispensing mechanism 120 , the driving mechanism 130 , and the detecting mechanism 140 .

[0070] The sample dispensing mechanism 120 is used to dispense the sample into the sample containing portion 101 .

[0071] For example, samples include, but are not limited to, at least one of the following: blood, ascites, sputum, and other bodily fluids, though this is not a limitation. Sample analyzers are used, for example, to detect specific proteins, peptides, small molecules, and the like in bodily fluids. The test results can reflect the physiological or pathological state of the human body, thereby providing a basis for clinical diagnosis and forming the foundation of evidence-based medicine. For example, sample analyzers include, but are not limited to, at least one of the following: a biochemical analyzer, an electrolyte analyzer, an immunoassay analyzer, a coagulation analyzer, and a urine analyzer. For example, the sample analyzer may be a biochemical analyzer, which may include at least one of an immunoassay module, a coagulation analysis module, and a urine analysis module.

[0072] For ease of description, the embodiments of the present application are mainly described using a whole blood sample and a sample analyzer such as an immunoassay analyzer as an example.

[0073] In some embodiments, as shown in FIG2 , the reagent kit 10 further includes a pipette head 12. For example, the reagent kit 10 further includes a pipette head receptacle 103, in which the pipette head 12 is pre-installed. A sample dispensing mechanism 120 can be connected to the pipette head 12 in the reagent kit 10, aspirating the sample via the pipette head 12 and adding the aspirated sample to the sample receptacle 101 of the reagent kit 10; the pipette head 12 can then be unloaded into the pipette head receptacle 103. Of course, this is not limiting. For example, in other embodiments, the sample dispensing mechanism 120 can add the aspirated sample to the sample receptacle 101 of the reagent kit 10 via a sample needle, and then clean the sample needle.

[0074] Optionally, the sample containing portion 101 contains a sample processing reagent, such as a whole blood sample processing reagent, which is used to pre-treat the added whole blood sample and can also be used to dilute the blood sample.

[0075] Optionally, when the sample dispensing mechanism 120 adds the sample into the sample holding portion 101 , the sample and the sample processing reagent may be mixed by aspirating and discharging the liquid in the sample holding portion 101 multiple times.

[0076] The drive mechanism 130 is used to drive the capture head 11 to move, and is at least capable of driving the capture head 11 to be placed in the sample holding portion 101 and the reagent holding portion 102 of the reagent cartridge 10. The drive mechanism 130 is, for example, a two-dimensional drive mechanism 130 or a three-dimensional drive mechanism 130, capable of driving the capture head 11 to move vertically and horizontally.

[0077] In some embodiments, as shown in FIG2 , the reagent kit 10 further includes a capture head accommodating portion 104, and the capture head 11 is pre-installed in the capture head accommodating portion 104. The driving mechanism 130 can be connected to the capture head 11 and drive the capture head 11 to move.

[0078] In other embodiments, as shown in Figure 3, the capture head 11 may be pre-installed in the sample receiving portion 101 of the reagent kit 10. The reagent kit 10 may not be provided with a separate capture head receiving portion 104 and may be shorter in length.

[0079] The controller 150 is used to control the driving mechanism 130 to place the capture head 11 in the sample of the sample holding portion 101 so that the capture head 11 captures the target substance in the sample; control the driving mechanism 130 to remove the capture head 11 from the sample, and control the driving mechanism 130 to place the capture head 11 in the reagent of the reagent holding portion 102 for reaction to obtain a product to be tested; and control the detection mechanism 140 to detect the product to be tested to obtain the detection result of the sample.

[0080] In some embodiments, the capture head 11 is pre-coated with a first substance to be bound, which can bind to the target substance in the sample. It should be noted that the binding can be physical adsorption or biochemical reaction binding, such as the binding of an antibody and an antigen.

[0081] In other embodiments, the controller 150 is further configured to control the drive mechanism 130 to place the capture head 11 in a solution containing a first substance to be bound, so that the capture head 11 is coated with the first substance to be bound, before the capture head 11 is placed in the sample in the sample holding portion 101. For example, the capture head 11 is pre-coated with nanospheres 1131. When the capture head 11 is placed in the solution containing the first substance to be bound, the first substance to be bound in the solution covalently couples with the nanospheres 1131 on the capture head 11, so that the capture head 11 is coated with the first substance to be bound.

[0082] Illustratively, the first substance to be bound is an antigen substance, and the capture head 11 can capture the target substance in the sample, such as an antibody; or the first substance to be bound is an antibody substance, and the capture head 11 can capture the target substance in the sample, such as an antigen.

[0083] In some embodiments, the reagent includes a second substance to be bound. When the capture head 11 is removed from the sample and placed in the reagent, the target substance captured by the capture head 11 binds to the second substance to be bound in the reagent to form a test product. Exemplarily, the reagent can be a secondary antibody reagent linked to a label, preferably an enzyme-labeled or acridinium ester-labeled secondary antibody reagent.

[0084] The detection mechanism 140 is used to detect the test product prepared based on the sample. For example, it can stimulate the test product, such as a marker bound to the target substance, to emit light and detect parameters such as the intensity of the light emission. Based on the detection results, for example, the concentration of the target substance in the sample can be determined.

[0085] The controller 150 is further configured to control the drive mechanism 130 to rotate the capture head 11 after the capture head 11 is removed from the sample and / or reagent. Specifically, the drive mechanism 130 can drive the capture head 11 to rotate about an axis. When the capture head 11 rotates, centrifugal force can remove droplets on the capture head 11.

[0086] Exemplarily, after the capture head 11 is removed from the sample, the drive mechanism 130 is controlled to rotate the capture head 11 to remove droplets from the capture head 11. For example, this can remove non-target substances, such as blood cells, from the capture head 11, increase the concentration of the target substance bound to the first substance to be bound, and prevent interference from non-target substances.

[0087] Exemplarily, as shown in FIG4 , the drive mechanism 130 is configured to drive the capture head 11 to rotate about a vertical axis when the capture head 11 is driven to rise above the liquid level of the sample in the sample holding portion 101 and the capture head 11 is at least partially located within the sample holding portion 101. By rotating within the sample holding portion 101 to remove droplets, the removed droplets remain within the sample holding portion 101, thereby preventing the droplets removed by the capture head 11 from contaminating the sample analyzer.

[0088] After the capture head 11 removes the sample and rotates to remove droplets on the capture head 11, the controller 150 controls the drive mechanism 130 to place the capture head 11 in the reagent for reaction to produce the test product. Because the concentration of the target substance bound to the first substance to be bound is high on the capture head 11, the concentration of the test product can be increased, improving the accuracy of sample detection.

[0089] In some embodiments, the reagent includes a second substance to be bound, and the controller 150 controls the driving mechanism 130 to place the capture head 11 in the reagent in the reagent holding portion 102 so that the target substance captured by the capture head 11 binds to the second substance to be bound in the reagent to form a product to be tested; then controls the driving mechanism 130 to remove the capture head 11 from the reagent; and after the capture head 11 is removed from the reagent, controls the driving mechanism 130 to drive the capture head 11 to rotate.

[0090] After the capture head 11 is taken out of the reagent, it is rotated to remove the droplets on the capture head 11. For example, the solvent and other substances other than the second substance to be bound in the reagent adsorbed by the capture head 11 can be removed, and the second substance to be bound that is not bound to the target substance can also be removed; the concentration of the second substance to be bound to the target substance on the capture head 11, that is, the product to be tested, can be increased to improve the accuracy of subsequent detection.

[0091] Exemplarily, as shown in FIG5 , the drive mechanism 130 is configured to drive the capture head 11 to rotate about a vertical axis when the capture head 11 is driven to rise above the liquid level of the reagent in the reagent holding section 102 and the capture head 11 is at least partially located within the reagent holding section 102. By rotating in the reagent holding section 102 to remove droplets, the removed droplets remain in the reagent holding section 102, thereby preventing the droplets removed by the capture head 11 from contaminating the sample analyzer.

[0092] It should be noted that, in some embodiments, the capture head 11 may not be driven to rotate after it is removed from the sample, and may be driven to rotate only after it is removed from the reagent. This can also reduce the interfering substances on the capture head 11 and increase the concentration of the product to be tested, thereby improving the accuracy of subsequent detection. In other embodiments, the capture head 11 may be driven to rotate after it is removed from the sample, and may not be driven to rotate after it is removed from the reagent. Since the rotation of the capture head 11 after it is removed from the sample reduces the interference of non-target substances in the sample, the concentration of the product to be tested is also higher after the capture head 11 is removed from the reagent, which can also improve the accuracy of subsequent detection.

[0093] In some embodiments, as shown in FIG. 2 , the reagent kit 10 further includes a substrate container 105 , in which a substrate solution is contained.

[0094] The controller 150 is also used to control the driving mechanism 130 to place the capture head 11 in the substrate liquid in the substrate holding portion 105 for rotation after the capture head 11 is removed from the reagent and rotated; the detection mechanism 140 is used to detect the capture head 11 and / or the liquid in the substrate holding portion 105 after the capture head 11 rotates in the substrate liquid.

[0095] The substrate liquid includes a substance that can excite the marker to emit light, such as hydrogen peroxide. The capture head 11 is removed from the reagent and placed in the substrate liquid. The substrate liquid can excite the second-generation binding substance on the capture head 11 that is bound to the target substance to emit light. The reaction efficiency and reaction degree can be improved by rotating the capture head 11 when the capture head 11 is placed in the substrate liquid. The detection mechanism 140 can detect the capture head 11 and the substrate liquid when the capture head 11 is placed in the substrate liquid, or can detect the capture head 11 after the capture head 11 is removed from the substrate liquid, or can detect the substrate liquid after the capture head 11 is removed from the substrate liquid.

[0096] Illustratively, a photometric portion is configured on the substrate receptacle 105. The photometric portion is, for example, made of a transparent material. For example, the reagent kit dispatching mechanism may dispatch the reagent kit 10 to the detection mechanism 140 such that the photodetector of the detection mechanism 140 is positioned adjacent to the photometric portion of the substrate receptacle 105, allowing the detection mechanism 140 to detect the substance in the substrate receptacle 105.

[0097] For example, as shown in FIG6 , the substrate holding portion 105 is movably arranged on the reagent kit 10, and the sample analyzer can also transport the substrate holding portion 105 to the detection mechanism 140 in the sample analyzer. The detection mechanism 140 can add the capture head 11 in the substrate holding portion 105 and / or the liquid in the substrate holding portion 105 into the photometric container A for photometric measurement. The structural design difficulty of the detection mechanism 140 is relatively simple.

[0098] In other embodiments, after the capture head 11 is removed from the reagent and rotated, the detection mechanism 140 can directly detect the product to be detected on the capture head 11, for example, by using a fluorescence method to detect the capture head 11; the capture head 11 may not need to react with the substrate liquid.

[0099] The sample analyzer of the embodiment of the present application removes interfering substances on the capture head 11, such as non-target substances in the sample or second substances to be bound that are not bound to the target substances, by driving the capture head 11 to rotate after the capture head 11 captures the target substance from the sample and removes it, and / or driving the capture head 11 to rotate after the capture head 11 reacts with the reagent and removes it, thereby reducing the interference of the interfering substances on the detection of the product to be tested and improving the accuracy of sample detection.

[0100] It can be understood that when the capture head 11 is driven to rotate to remove the droplets on the capture head 11, the substances that have not yet bound on the capture head 11 still have a high probability of binding, which can increase the content of the substances bound on the capture head 11, such as increasing the target substance bound to the first substance to be bound on the capture head 11, and increasing the content of the second substance to be bound to the target substance, that is, the content of the product to be tested on the capture head 11 can be increased, and the accuracy of sample detection can be improved.

[0101] In some embodiments, as shown in FIG. 7 and FIG. 8 , the capturing head 11 includes a connecting portion 111 and a capturing portion 112 .

[0102] The connecting portion 111 is used to connect with the driving mechanism 130 . For example, the clamping portion of the driving mechanism 130 can clamp the connecting portion 111 of the capture head 11 . Of course, it is not limited thereto, and for example, a magnetic connection can be used.

[0103] The capture portion 112 is connected to the connecting portion 111 or is integrally formed, and the capture portion 112 forms a plurality of surface contact structures 1121, and the surface area of ​​the capture head 11 is 1 to five times the surface area of ​​a cylinder of the same length and width; preferably, it is three times the surface area of ​​a cylinder of the same length and width.

[0104] By forming multiple surface contact structures 1121 on the capture portion 112, the surface area of ​​the capture head 11 can be increased. With this increased surface area, the capture head 11 can, for example, coat more of the first substance to be bound, more efficiently capturing more target substances in the sample. Furthermore, during the reaction with the reagent, for example, it can more efficiently bind with more of the second substance to be bound, thereby generating more test products on the capture head 11. This improves the efficiency and sensitivity of substance capture, and can enhance the efficiency and accuracy of sample testing.

[0105] Moreover, the larger surface area of ​​the capture head 11 allows more test substances to remain on the capture head 11 after the capture head 11 is removed from the sample and / or reagent and rotated, and by removing interfering substances, the accuracy of detection can be improved and the sensitivity of capturing the test product can be increased.

[0106] In some embodiments, the controller 150 is used to control the driving mechanism 130 to move the capture head 11 to the sample in the sample holding portion 101, the reagent in the reagent holding portion 102, and the detection mechanism 140 in sequence, so that the capture head 11 captures the target substance in the sample, the target substance captured by the capture head 11 reacts with the reagent to obtain a product to be tested, and the detection mechanism 140 is controlled to detect the product to be tested, so as to obtain the test result of the sample in one test.

[0107] By driving the capture head 11 to rotate after it is removed from the sample and / or reagent to remove interfering substances, and / or increasing the surface area of ​​the capture head 11 to increase the efficiency and yield of substance binding, the capture head 11 can be placed in the sample once and in the reagent once, and the product to be tested on the capture head 11 can meet the detection requirements of the detection mechanism 140. For example, if the concentration of the marker is high enough after the capture head 11 is placed in the substrate solution, the detection mechanism 140 can accurately determine the concentration of the target substance in the sample by detecting the luminescence data of the marker. Compared with placing the capture head 11 in the sample and reagent for multiple cycles to meet the detection requirements, the detection efficiency of the embodiment of the present application is higher.

[0108] Exemplarily, the length between one end of the connecting portion 111 away from the capturing portion 112 and one end of the capturing portion 112 away from the connecting portion 111 is 5 mm to 50 mm, preferably 20 mm.

[0109] Exemplarily, the width of the capture portion 112 projected along the axial direction of the capture head 11 is 2 mm to 8 mm, preferably 5 mm. For example, the length between the end of the connecting portion 111 away from the capture portion 112 and the end of the capture portion 112 away from the connecting portion 111 is 5 mm to 50 mm, and the width of the capture portion 112 projected along the axial direction of the capture head 11 is 2 mm to 8 mm.

[0110] By forming a plurality of surface contact structures 1121 in the capture portion 112, the surface area of ​​the capture head 11 is increased, so that when the length and / or width of the capture head 11 is relatively small, more substances can still be captured to meet the detection requirements.

[0111] In some embodiments, the surface contact structure 1121 includes at least one of the following: protrusions and openings. For example, the protrusions are selected from at least one of the following: spherical protrusions, annular protrusions, and cylindrical protrusions; and the openings are selected from at least one of the following: through holes, blind holes, and cross-linked holes.

[0112] As shown in FIG7 , the capture portion 112 includes a columnar body 112A, with at least a portion of a spherical protrusion formed on the side of the columnar body 112A. Optionally, the end of the columnar body 112A facing away from the connecting portion 111 may have a spherical surface 112B, and at least a portion of the spherical protrusion may also be formed on the spherical surface 112B. This allows the capture portion 112 to be formed into a raspberry shape, thereby increasing the surface area of ​​the capture head 11.

[0113] Optionally, the diameter of the spherical protrusions is 0.2 mm to 2 mm. For example, dense hemispherical protrusions can be generated on the columnar body 112A of the capture portion 112 by processing or injection molding, which can increase the surface area of ​​the originally smooth columnar body 112A to about twice the original size.

[0114] For example, as shown in FIG8 , the capture portion 112 includes a support rod 112C, one end of which is connected to or integrally formed with the connection portion 111. The support rod 112C has a plurality of annular protrusions formed in its axial direction as surface contact structures 1121, with adjacent annular protrusions spaced apart. This allows the capture head 11 to have a multi-layer sheet-like structure.

[0115] 9A , the surface contact structure 1121 formed by the capture portion 112 includes a turbine-shaped protrusion; as shown in FIG9B , the surface contact structure 1121 formed by the capture portion 112 includes a cylindrical protrusion; as shown in FIG9C , the surface contact structure 1121 formed by the capture portion 112 includes a through hole formed by the capture portion 112.

[0116] Illustratively, the surface of the capture portion 112 is further formed with a coating layer (not shown), which includes a first substance to be bound and / or a substance capable of binding to the first substance to be bound. The first substance to be bound can bind to a target substance in the sample. It should be noted that the binding can be physical adsorption or biochemical reaction binding, such as the binding of an antibody and an antigen.

[0117] Exemplarily, the capture portion 112 is made of a non-metallic material; preferably, the non-metallic material is selected from a polymer and silicon dioxide, and the polymer is selected from at least one of the following: polyethylene, polypropylene, and polystyrene. Thus, a coating layer is formed on the surface of the capture portion 112.

[0118] For example, the first substance to be bound can be coated on the surface of the capture portion 112 by adsorption or covalent bonding. Taking the first substance to be bound as an antibody as an example, the process of forming a coating layer on the surface of the capture portion 112 is as follows:

[0119] 1. Coating: Immerse the capture head 11 in the avidin-carbonate buffer solution to coat the avidin. Incubate at 4°C overnight.

[0120] 2. Blocking: Remove the capture head 11 from the coating system solution and wash it three times with the cleaning solution; add the blocking solution and incubate at 37°C for 2 hours;

[0121] 3. Drying: Remove the capture head 11 from the blocking solution, dry it by absorbing the water, and dry it at 37°C;

[0122] 4. Capture antibody incubation: Immerse the capture head 11 in the "capture antibody-biotin" working solution; incubate at 37° C. for 2 h; then remove the capture head 11 from the "capture antibody-biotin" working solution.

[0123] In some embodiments, as shown in Figure 10, the coating layer includes nanospheres 1131 and a first substance to be bound to the nanospheres 1131. For example, the nanospheres 1131 can be coupled to the surface contact structure 1121 of the capture head 11 by covalent coupling, and then the antigen or antibody Ab can be coated on this layer of nanospheres 1131. Optionally, the material of the nanospheres 1131 can be a polymer such as polyethylene, polypropylene, or styrene. The diameter of the nanospheres 1131 can range from 100 nanometers to 2000 nanometers. By binding the first substance to be bound to the nanospheres 1131, the coating area of ​​the first substance to be bound to the capture head 11 can be increased to about 8 times that of the smooth cylindrical rod.

[0124] Exemplarily, the smooth transition between the plurality of surface contact structures 1121 can allow the first substance to be bound to stably form a coating layer on the capture head 11 .

[0125] In some embodiments, referring to FIG2 , the reagent kit 10 further includes a cleaning liquid container 106 containing cleaning liquid. The reagent kit 10 may include one or more cleaning liquid containers 106 , for example, different cleaning liquid containers 106 are used to clean the capture head 11 at different stages of the assay.

[0126] Exemplarily, the controller 150 is also used to control the drive mechanism 130 to place the capture head 11 in the cleaning liquid of the cleaning liquid holding portion 106 for cleaning before the capture head 11 is placed in the reagent, so that after the capture head 11 is taken out of the sample, interfering substances on the capture head 11, such as non-target substances, can be removed by cleaning. Optionally, the capture head 11 can be placed in the cleaning liquid of the cleaning liquid holding portion 106 and rotated to improve the efficiency of cleaning. After the capture head 11 is cleaned, the drive mechanism 130 is controlled to remove the capture head 11 from the cleaning liquid, and after the capture head 11 is removed from the cleaning liquid, the drive mechanism 130 is controlled to drive the capture head 11 to rotate to remove at least part of the droplets on the capture head 11, such as the cleaning liquid on the capture head 11, so as to increase the concentration of the target substance on the capture head 11.

[0127] Exemplarily, the controller 150 is further configured to control the drive mechanism 130 to place the capture head 11 in the cleaning liquid in the cleaning liquid container 106 and rotate it after the capture head 11 is removed from the reagent. This is to efficiently clean the capture head 11 and remove interfering substances on the capture head 11, such as removing a second substance to be bound that is not bound to the target substance. After the capture head 11 is cleaned, the drive mechanism 130 is controlled to remove the capture head 11 from the cleaning liquid. After the capture head 11 is removed from the cleaning liquid, the drive mechanism 130 is controlled to drive the capture head 11 to rotate to remove at least some droplets on the capture head 11, such as the cleaning liquid on the capture head 11, thereby increasing the concentration of the product to be detected on the capture head 11.

[0128] Exemplarily, as shown in FIG11 , the drive mechanism 130 is configured to rotate the capture head 11 about a vertical axis when the capture head 11 is driven to rise above the level of the cleaning fluid in the cleaning fluid container 106 of the reagent cartridge 10 and the capture head 11 is at least partially located within the cleaning fluid container 106. By rotating within the cleaning fluid container 106 to remove droplets, the removed droplets remain within the cleaning fluid container 106, thereby preventing the droplets removed by the capture head 11 during rotation from contaminating the sample analyzer.

[0129] In other embodiments, the sample analyzer further includes a cleaning mechanism (not shown). For example, the cleaning mechanism includes a cleaning container, and the cleaning liquid in the cleaning container can be used to clean the capture head 11; for example, the driving mechanism 130 can place the capture head 11 in the cleaning liquid in the cleaning container for rotation. It is also used to control the driving mechanism 130 to place the capture head 11 in the cleaning mechanism for cleaning before the capture head 11 is placed in the reagent, and / or after the capture head 11 is taken out of the reagent and rotated, so as to remove interfering substances on the capture head 11. After the capture head 11 is cleaned, the driving mechanism 130 can be controlled again to drive the capture head 11 to rotate to remove at least some droplets on the capture head 11, such as cleaning liquid. As shown in Figure 12, the cleaning liquid holding portion 106 may not be provided on the reagent reagent 10, or fewer cleaning liquid holding portions 106 may be provided, so as to reduce the length of the reagent reagent 10; the reagent reagent storage area 110 of the same size can load more reagent reagents 10.

[0130] In some embodiments, the controller 150 is further configured to control the drive mechanism 130 to rotate the capture head 11 when the capture head 11 is placed in at least one of a sample, a reagent, a cleaning solution, and a substrate solution. This ensures that substances on the capture head 11 are in full contact with substances in the sample, reagent, cleaning solution, substrate solution, and other liquids, thereby improving reaction or cleaning efficiency. This also reduces the time the capture head 11 remains in the sample, reagent, cleaning solution, or substrate solution, thereby improving sample detection efficiency.

[0131] Exemplarily, the rotational speed of the capture head 11 when it is removed from the sample and / or reagent to remove at least some droplets is greater than the rotational speed of the capture head 11 when it is placed within the sample and / or reagent to improve reaction efficiency. Removing droplets from the capture head 11 at a higher rotational speed through centrifugal force can more effectively remove interfering substances on the capture head 11. Rotating at a lower rotational speed within the sample or reagent ensures that substances contacted by the capture head 11 can fully react with substances on the capture head 11.

[0132] For example, the rotation speed of the capture head 11 when it is taken out of the sample and / or reagent is greater than or equal to 10 revolutions per second and less than or equal to 100 revolutions per second; the rotation speed of the capture head 11 when it is placed in the sample and / or reagent is greater than or equal to 1 revolution per second and less than or equal to 10 revolutions per second.

[0133] For example, the controller 150 controls the driving mechanism 130 to drive the capture head 11 to rotate for 0.6 seconds to 60 seconds after the capture head 11 is taken out of the sample and / or reagent; when the capture head 11 is placed in the sample and / or reagent, the controller 150 controls the driving mechanism 130 to drive the capture head 11 to rotate for 6 seconds to 5 minutes.

[0134] In some embodiments, as shown in FIG13 , the reagent cartridge 10 further includes a droplet receiving portion 107. After the capture head 11 is removed from the sample and / or reagent, the controller 150 controls the drive mechanism 130 to place the capture head 11 in the cavity of the droplet receiving portion 107, and controls the drive mechanism 130 to rotate the capture head 11 within the droplet receiving portion 107. By driving the capture head 11 to rotate within the droplet receiving portion 107 on the reagent cartridge 10 to remove at least some droplets from the capture head 11, and by allowing the droplet receiving portion 107 to collect droplets that fall from the capture head 11, droplets removed during rotation of the capture head 11 can be prevented from contaminating the sample analyzer. Please refer to FIG13 in conjunction with FIG4 and FIG5. Since there is liquid in the sample holding portion 101 and the reagent holding portion 102 and the liquid level is high, the capture head 11 needs to be raised to a higher height to rotate and remove the droplets. In the droplet holding portion 107, the capture head 11 can rotate and remove the droplets at a lower height. Therefore, the height of the reagent kit 10 can be lowered. The reagent kit storage area 110 of the same size can load more reagent kits 10.

[0135] Optionally, as shown in FIG13 , a buffer structure 1071 is formed on the inner wall of the cavity of the droplet receiving portion 107. The buffer structure 1071 can be, for example, a mesh or fleece structure. This prevents droplets from continuously bouncing off the inner wall of the droplet receiving portion 107 and splashing when the capture head 11 rotates in the droplet receiving portion 107 to remove droplets, thereby preventing incomplete droplet removal.

[0136] Optionally, a buffer structure 1071 may also be formed on the inner wall of the cavity of the sample holding portion 101 and / or the inner wall of the cavity of the reagent holding portion 102 to prevent the droplets from continuously rebounding and splashing on the inner wall of the droplet holding portion 107 when the capture head 11 rotates in the sample holding portion 101 or the reagent holding portion 102 to remove the droplets, resulting in incomplete droplet removal.

[0137] Optionally, the diameter of the opening of the droplet receiving portion 107 is larger than the diameter of the bottom of the cavity of the droplet receiving portion 107. Increasing the space of the droplet receiving portion 107 can also facilitate the collection of droplets on the inner wall of the droplet receiving portion 107 to the bottom, preventing them from being contaminated again on the capture head 11.

[0138] Exemplarily, the droplet receiving portion 107 and the sample receiving portion 101 are the same receiving portion on the reagent cartridge 10. Specifically, the sample receiving portion 101 can serve as the droplet receiving portion 107. After the capture head 11 is removed from the reagent or cleaning solution, the drive mechanism 130 is controlled to rotate the capture head 11 within the sample receiving portion 101 to remove droplets from the capture head 11. This eliminates the need for a separate droplet receiving portion 107 on the reagent cartridge 10, thereby reducing the length of the reagent cartridge 10.

[0139] For example, as shown in FIG13 , the droplet holding portion 107 and the sample holding portion 101 are separate holding portions on the reagent cartridge 10, and the diameter of the cavity of the droplet holding portion 107 is greater than or equal to the diameter of the cavity of the sample holding portion 101 at at least one location. Providing a droplet holding portion 107 with a larger aperture on the reagent cartridge 10 can prevent droplets removed by the capture head 11 from rebounding and fogging on the inner wall, or from contaminating the capture head 11 with droplets on the inner wall.

[0140] In other embodiments, the sample analyzer further includes a droplet collection container (not shown). After the capture head 11 is removed from the sample and / or reagent, the controller 150 controls the drive mechanism 130 to place the capture head 11 in the cavity of the droplet collection container and to control the drive mechanism 130 to rotate the capture head 11 within the droplet collection container. This eliminates the need for a separate droplet receiving portion 107 on the reagent cartridge 10, thereby reducing the length of the reagent cartridge 10.

[0141] Optionally, a buffer structure 1071 is formed on the inner wall of the cavity of the droplet collection container. The buffer structure 1071 is, for example, a mesh or fleece structure. This prevents the droplets from continuously bouncing and splashing off the inner wall of the droplet collection container when the capture head 11 rotates in the droplet collection container to remove the droplets, resulting in incomplete droplet removal.

[0142] Optionally, the diameter of the opening side of the droplet collection container is larger than the diameter of the bottom of the cavity of the droplet collection container, so that the droplets on the inner wall of the droplet collection container can be gathered at the bottom to prevent them from being contaminated by the capture head 11 again.

[0143] Optionally, the diameter of the cavity of the droplet collection container at least at one location is greater than or equal to the diameter of the cavity of the sample holding portion 101. By using a droplet collection container with a larger aperture, it is possible to prevent droplets removed from the capture head 11 from rebounding on the inner wall to form mist, or to prevent droplets on the inner wall from contaminating the capture head 11.

[0144] In some embodiments, as shown in FIG14 , the capture head 11 is pre-installed in the droplet receiving portion 107 of the reagent kit 10 , that is, the droplet receiving portion 107 and the capture head receiving portion 104 can be the same, thereby reducing the length of the reagent kit 10 .

[0145] The sample analyzer provided in the embodiment of the present application removes interfering substances on the capture head 11 by driving the capture head 11 to rotate after capturing the target substance from the sample and removing it, and / or driving the capture head 11 to rotate after the capture head 11 reacts with the reagent and removes it, thereby reducing the interference of the interfering substances on the detection of the product to be tested and improving the accuracy of sample detection.

[0146] In some embodiments, by forming multiple surface contact structures 1121 on the capture portion 112, the surface area of ​​the capture head 11 is increased. For example, the number of antibodies or antigens coated on the capture head 11 is increased, allowing it to bind to more target substances in the sample and the second substance to be bound in the reagent. This improves the efficiency and sensitivity of substance capture, thereby enhancing the efficiency and accuracy of sample detection. This allows the capture head 11 to be placed once in the sample and once in the reagent, and the product to be detected on the capture head 11 can meet the detection requirements of the detection mechanism 140. Compared to requiring the capture head 11 to be placed in the sample and reagent multiple times to meet the detection requirements, the detection efficiency of the embodiments of the present application is higher.

[0147] In some embodiments, the larger surface area of ​​the capture head 11 allows more test substances to remain on the capture head 11 after the capture head 11 is removed from the sample and / or reagent and rotated, and by removing interfering substances, the accuracy of detection can be improved and the sensitivity of capturing the test product can be increased.

[0148] In some embodiments, when the capture head 11 is immersed in the sample, reagent or substrate liquid, it is driven to rotate around the axis to improve the reaction efficiency; when the capture head 11 is immersed in the cleaning liquid and rotated around the axis at the same time, the cleaning efficiency can be improved.

[0149] In some other embodiments of the present application, droplets on the capture head 11 can be removed by a droplet removal mechanism 160. Please refer to Figure 15 in conjunction with the aforementioned embodiment. The sample analyzer also includes a droplet removal mechanism 160, which is used to remove droplets on the capture head 11. Specifically, the controller 150 is at least used to control the droplet removal mechanism 160 to remove at least some of the droplets on the capture head 11 after the capture head 11 is removed from the sample and / or reagent. Alternatively, the droplet removal mechanism 160 can also be controlled to remove at least some of the droplets on the capture head 11 after the capture head 11 is removed from the substrate liquid or the cleaning liquid.

[0150] Exemplarily, the liquid droplet removal mechanism 160 is used to provide airflow to the capture head 11 to remove liquid droplets on the capture head 11 .

[0151] In other embodiments of the present application, the sample and reagent can be placed in the same container 101a, and the capture head 11 can be placed in the container 101a containing the sample and the reagent so that the capture head 11 captures the target substance in the sample, and the reaction is carried out in the container 101a to obtain the product to be tested. This can improve detection efficiency.

[0152] Specifically, please refer to FIG. 17 in conjunction with the above embodiment. The reagent kit 10 is constructed to include a receiving portion 101 a having an opening, and a capture head 11 is pre-installed on the reagent kit 10.

[0153] The capture head 11 includes a capture portion 112, the capture portion 112 forms a plurality of surface contact structures 1121, and the surface area of ​​the capture head 11 is 1 to 5 times the surface area of ​​a cylinder of the same length and width;

[0154] The receiving portion 101a is used to receive samples and reagents, and is placed in the capture head 11 so that the capture portion 112 captures the target substance in the sample and the target substance captured by the capture head 11 reacts with the reagent to obtain a product to be tested.

[0155] Specifically, the sample analyzer includes:

[0156] A reagent kit 10 storage area, wherein at least one reagent kit 10 can be placed in the reagent kit 10, and the reagent kit 10 includes a receiving portion 101a and a capture head 11;

[0157] A dispensing mechanism for dispensing a sample into the containing portion 101 a, wherein the containing portion 101 a is pre-filled with a reagent; or the containing portion 101 a includes a sample containing portion 101 and a reagent containing portion 102, wherein the reagent containing portion 102 is pre-filled with a reagent, and the dispensing mechanism is used to dispense the sample into the sample containing portion 101 and to dispense the reagent in the reagent containing portion 102 into the sample containing portion 101;

[0158] A driving mechanism 130, wherein the driving mechanism 130 is used to drive the capture head 11 to move;

[0159] A detection mechanism 140, the detection mechanism 140 is used to detect the product to be tested prepared based on the sample;

[0160] The controller 150 is used to control the driving mechanism 130 to place the capture head 11 in the containing portion 101a including the sample and the reagent so that the capture head 11 captures the target substance in the sample, and to react in the containing portion 101a to obtain the product to be tested; and control the detection mechanism 140 to detect the product to be tested to obtain the detection result of the sample.

[0161] For example, the reagent kit 10 may not be provided with a sample holding portion 101, and the dispensing mechanism (such as the sample dispensing mechanism 120) may directly dispense the sample into the reagent holding portion 102; the controller 150 is configured to control the driving mechanism 130 to place the capture head 11 into the reagent holding portion 102 containing the sample and the reagent, so that the capture head 11 captures the target substance in the sample and reacts in the reagent holding portion 102 to obtain the test product; and the detection mechanism 140 is configured to detect the test product to obtain the test result of the sample. This can reduce the length of the reagent kit 10.

[0162] Exemplarily, a sample holding portion 101 and a reagent holding portion 102 are provided on the reagent cartridge 10, and the reagent holding portion 102 is pre-loaded with a reagent; after the dispensing mechanism dispenses the sample into the sample holding portion 101, the dispensing mechanism then adds the reagent in the reagent holding portion 102 into the sample holding portion 101. The dispensing mechanism for dispensing the sample and the dispensing mechanism for dispensing the reagent can be the same dispensing mechanism or different dispensing mechanisms; or the dispensing mechanism can be connected to different pipetting heads 12 to dispense the sample and the reagent respectively. The controller 150 is used to control the driving mechanism 130 to place the capture head 11 in the sample holding portion 101 including the sample and the reagent so that the capture head 11 captures the target substance in the sample, and to react in the sample holding portion 101 to obtain the product to be tested; and to control the detection mechanism 140 to detect the product to be tested to obtain the detection result of the sample.

[0163] In some embodiments, the controller 150 is further configured to control the driving mechanism 130 to drive the capturing head 11 to rotate after the capturing head 11 is taken out of the liquid in the receiving portion 101 a .

[0164] In some embodiments, the sample analyzer further includes a droplet removal mechanism for removing droplets from the capture head 11; the controller 150 is further configured to control the droplet removal mechanism to remove at least some of the droplets from the capture head 11 after the capture head 11 is removed from the liquid in the container 101a.

[0165] It is understandable that the embodiment of the present application further provides a reagent kit 10. Please refer to Figure 16 in combination with the above embodiment. The reagent kit 10 is constructed to include a sample holding portion 101 with an opening and a reagent holding portion 102, and a capture head 11 is pre-installed on the reagent kit 10.

[0166] The capture head 11 includes a capture portion 112, the capture portion 112 forms a plurality of surface contact structures 1121, and the surface area of ​​the capture head 11 is 1 to 5 times the surface area of ​​a cylinder of the same length and width;

[0167] The sample receiving portion 101 is used to receive the sample and is placed in the capture head 11 so that the capture portion 112 can capture the target substance in the sample.

[0168] The reagent containing portion 102 is filled with a reagent, and is for the capture head 11 to be placed therein so that the target substance captured by the capture head 11 reacts with the reagent to obtain a product to be tested.

[0169] It can be understood that the embodiment of the present application also provides a capture head 11. Please refer to Figure 18 in combination with the aforementioned embodiment. The capture head 11 includes a connecting portion 111 and a capture portion 112. The capture portion 112 is connected to the connecting portion 111 or is integrally formed; the capture portion 112 forms a plurality of surface contact structures 1121, and the surface area of ​​the capture head 11 is 1 to five times the surface area of ​​a cylinder of the same length and the same width.

[0170] A coating layer is further formed on the surface of the capturing portion 112 . The coating layer includes a first substance to be bound and / or a substance capable of binding to the first substance to be bound. The first substance to be bound can bind to a target substance in the sample.

[0171] Please refer to FIG. 19 in conjunction with the aforementioned embodiment. FIG. 19 is a flow chart of a control method of a sample analyzer provided in one embodiment of the present application.

[0172] As shown in FIG. 19 , the control method of the sample analyzer includes the following steps S110 to S150 , and step S101 and / or step S102 .

[0173] Step S110, controlling the sample dispensing mechanism to dispense the sample into the sample receiving portion of the reagent cartridge;

[0174] Step S120, controlling the driving mechanism to place the capture head into the sample in the sample holding portion so that the capture head captures the target substance in the sample;

[0175] Step S130: controlling the driving mechanism to remove the capture head from the sample;

[0176] Step S140: Control the driving mechanism to place the capture head in the reagent of the reagent container of the reagent kit to react and obtain a product to be tested;

[0177] Step S150: Control the detection mechanism to detect the product to be tested to obtain the test result of the sample to be tested;

[0178] Step S101: after the capture head is removed from the sample, removing at least part of the droplets on the capture head; and / or

[0179] Step S102: After the reagent is taken out from the capture head, at least part of the droplets on the capture head are removed.

[0180] In some embodiments, after the capture head is removed from the sample and / or the reagent, the driving mechanism is controlled to drive the capture head to rotate so as to remove at least part of the droplets on the capture head.

[0181] In some embodiments, before placing the capture head into the reagent in the reagent receptacle of the reagent reagent cartridge, and / or after the capture head extracts and removes at least a portion of the droplet from the reagent, the method further comprises:

[0182] Controlling the driving mechanism to place the capture head in a cleaning solution for cleaning;

[0183] controlling the driving mechanism to remove the capture head from the cleaning liquid;

[0184] After the capture head is taken out of the cleaning liquid, at least part of the liquid droplets on the capture head are removed.

[0185] The specific principles and implementation methods of the control method of the sample analyzer provided in the embodiment of the present application are similar to those of the sample analyzer in the aforementioned embodiment, and will not be repeated here.

[0186] It should be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application.

[0187] It will also be understood that the term "and / or" as used in this application and the appended claims refers to and includes any and all possible combinations of one or more of the associated listed items.

[0188] The above description is merely a specific embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in the present application, and such modifications or substitutions should be included in the scope of protection of the present application. Therefore, the scope of protection of the present application should be based on the scope of protection of the claims.

Claims

1. A sample analyzer, characterized in that: include: A reagent kit storage area, wherein at least one reagent kit can be placed in the reagent kit storage area, wherein the reagent kit comprises a sample receiving portion, a capture head and a reagent receiving portion, wherein the reagent receiving portion is filled with a reagent; A sample dispensing mechanism, the sample dispensing mechanism is used to dispense the sample into the sample containing portion; A driving mechanism, the driving mechanism is used to drive the capture head to move; A detection mechanism, the detection mechanism is used to detect the product to be tested prepared based on the sample; A controller, the controller is used to control the driving mechanism to place the capture head in the sample of the sample holding part so that the capture head captures the target substance in the sample; control the driving mechanism to take the capture head out of the sample, and control the driving mechanism to place the capture head in the reagent of the reagent holding part to react and obtain the product to be tested; control the detection mechanism to detect the product to be tested to obtain the detection result of the sample; The controller is also used to control the driving mechanism to drive the capture head to rotate after the capture head is taken out from the sample and / or the reagent.

2. The sample analyzer according to claim 1, characterized in that: The capture head is pre-coated with a first substance to be combined, or the controller is further used to control the driving mechanism to place the capture head in a solution containing the first substance to be combined before the capture head is placed in the sample of the sample holding portion so that the capture head is coated with the first substance to be combined; the first substance to be combined can be combined with the target substance in the sample; After the capture head is taken out from the sample, the controller controls the driving mechanism to drive the capture head to rotate; Then, the driving mechanism is controlled to place the capture head in the reagent to react and obtain the product to be tested.

3. The sample analyzer according to claim 1, characterized in that: The reagent includes a second substance to be combined, and the controller controls the driving mechanism to place the capture head in the reagent of the reagent containing part so that the target substance captured by the capture head combines with the second substance to be combined in the reagent to form the product to be tested; Then controlling the driving mechanism to remove the capture head from the reagent; And after the capture head is taken out from the reagent, the driving mechanism is controlled to drive the capture head to rotate.

4. The sample analyzer according to claim 3, characterized in that: The kit further comprises a substrate containing portion, wherein the substrate containing portion is filled with a substrate liquid; The controller is also used to control the driving mechanism to place the capture head in the substrate liquid of the substrate containing part to rotate after the capture head is taken out of the reagent and rotated; The detection mechanism is used to detect the liquid in the capture head and / or the substrate containing part after the capture head rotates in the substrate liquid.

5. The sample analyzer according to claim 3, characterized in that: The kit further comprises a cleaning liquid containing portion, wherein the cleaning liquid containing portion is filled with cleaning liquid; The controller is also used to control the driving mechanism to place the capture head in the cleaning liquid in the cleaning liquid holding part for rotation before the capture head is placed in the reagent and / or after the capture head is taken out of the reagent and rotated, and then control the driving mechanism to take the capture head out of the cleaning liquid, and after the capture head is taken out of the cleaning liquid, control the driving mechanism to drive the capture head to rotate.

6. The sample analyzer according to claim 3, characterized in that: The sample analyzer further comprises a cleaning mechanism, which is further used to control the driving mechanism to place the capture head in the cleaning mechanism for cleaning before the capture head is placed in the reagent and / or after the capture head is taken out of the reagent and rotated.

7. The sample analyzer according to any one of claims 1 to 6, characterized in that: The controller is also used to control the driving mechanism to drive the capture head to rotate when the capture head is placed in at least one of the sample, the reagent, the cleaning solution, and the substrate solution.

8. The sample analyzer according to claim 7, characterized in that: The rotation speed of the capture head when it is rotated after being taken out of the sample and / or the reagent is greater than the rotation speed of the capture head when it is placed in the sample and / or the reagent and rotates.

9. The sample analyzer according to claim 8, characterized in that: The rotation speed of the capture head when it is rotated after being taken out from the sample and / or the reagent is greater than or equal to 10 revolutions per second and less than or equal to 100 revolutions per second; The rotation speed of the capture head when placed in the sample and / or the reagent and rotating is greater than or equal to 1 revolution per second and less than or equal to 10 revolutions per second.

10. The sample analyzer according to claim 7, characterized in that: The controller controls the driving mechanism to drive the capture head to rotate for 0.6 seconds to 60 seconds after the capture head is taken out of the sample and / or the reagent; and the controller controls the driving mechanism to drive the capture head to rotate for 6 seconds to 5 minutes when the capture head is placed in the sample and / or the reagent.

11. The sample analyzer according to any one of claims 1 to 6, characterized in that: The driving mechanism is used to drive the capture head to rotate around a vertical axis when the capture head is driven to rise above the liquid level of the sample in the sample holding portion and the capture head is at least partially located in the sample holding portion; and / or The driving mechanism is used to drive the capture head to rotate around a vertical axis when the capture head is driven to rise above the liquid level of the reagent in the reagent containing part and the capture head is at least partially located in the reagent containing part; and / or The driving mechanism is used to drive the capture head to rotate around a vertical axis when the capture head is driven to rise above the liquid level of the cleaning liquid in the cleaning liquid containing part of the reagent kit and the capture head is at least partially located in the cleaning liquid containing part.

12. The sample analyzer according to any one of claims 1 to 6, characterized in that: The reagent kit further comprises a liquid droplet receiving portion, and the controller is used to control the driving mechanism to place the capture head in the cavity of the liquid droplet receiving portion after the capture head is taken out from the sample and / or the reagent, and to control the driving mechanism to drive the capture head to rotate in the liquid droplet receiving portion; or The sample analyzer also includes a droplet collection container, and the controller is used to control the driving mechanism to place the capture head in the cavity of the droplet collection container after the capture head is taken out of the sample and / or the reagent, and to control the driving mechanism to drive the capture head to rotate in the droplet collection container.

13. The sample analyzer according to claim 12, characterized in that: A buffer structure is formed on the inner wall of the cavity of the liquid drop receiving portion or the liquid drop collecting container.

14. The sample analyzer according to claim 12, characterized in that: The diameter of the opening side of the droplet receiving portion is greater than the diameter of the bottom of the cavity of the droplet receiving portion; or The diameter of the open side of the droplet collecting container is greater than the diameter of the bottom of the cavity of the droplet collecting container.

15. The sample analyzer according to claim 12, characterized in that: The droplet receiving portion and the sample receiving portion are the same receiving portion on the reagent kit; or, The droplet receiving portion and the sample receiving portion are different receiving portions on the reagent kit. The diameter of the cavity of the droplet receiving portion at least at one location is greater than or equal to the diameter of the cavity of the sample receiving portion.

16. The sample analyzer according to any one of claims 1 to 6, characterized in that: The capture head is pre-set in the sample receiving portion of the reagent kit; or, The kit further comprises a capture head accommodating portion, in which the capture head is pre-arranged.

17. The sample analyzer according to claim 13, characterized in that: The capture head is pre-set in the liquid droplet receiving portion of the reagent cartridge.

18. The sample analyzer according to any one of claims 1 to 6, characterized in that: The capture head comprises: A connecting portion, the connecting portion being used to connect with the driving mechanism; A capture portion, the capture portion is connected to the connection portion or is integrally formed, the capture portion forms a plurality of surface contact structures, and the surface area of ​​the capture head is 1 to 5 times the surface area of ​​a cylinder of the same length and the same width; A coating layer is also formed on the surface of the capture part, and the coating layer includes a first substance to be bound and / or a substance capable of binding to the first substance to be bound, and the first substance to be bound can bind to a target substance in the sample.

19. The sample analyzer according to claim 18, characterized in that: The surface contact structure includes at least one of the following: a protrusion, an opening, and the protrusion is selected from at least one of the following: a spherical protrusion, an annular protrusion, and a columnar protrusion.

20. The sample analyzer according to claim 18, characterized in that: There is a smooth transition between the plurality of surface contact structures.

21. The sample analyzer according to claim 18, characterized in that: The length between one end of the connecting portion away from the capturing portion and one end of the capturing portion away from the connecting portion is 5 mm to 50 mm; and / or The width of the projection of the capturing portion along the axial direction of the capturing head is 2 mm to 8 mm.

22. The sample analyzer according to claim 18, characterized in that: The capture part is a structure made of non-metallic material; preferably, the non-metallic material is selected from high molecular polymer and silicon dioxide, and the high molecular polymer is selected from at least one of the following: polyethylene, polypropylene, polystyrene.

23. The sample analyzer according to claim 18, characterized in that: The coating layer includes nano-microspheres and the first substance to be bound to the nano-microspheres.

24. A sample analyzer, characterized in that: include: A reagent kit storage area, wherein at least one reagent kit can be placed in the reagent kit storage area, wherein the reagent kit comprises a sample receiving portion, a capture head and a reagent receiving portion, wherein the reagent receiving portion is filled with a reagent; A sample dispensing mechanism, the sample dispensing mechanism is used to dispense the sample into the sample containing portion; A driving mechanism, the driving mechanism is used to drive the capture head to move; A detection mechanism, the detection mechanism is used to detect the product to be tested prepared based on the sample; A controller is used to control the driving mechanism to sequentially move the capture head to the sample in the sample holding portion, the reagent in the reagent holding portion, and the detection mechanism, so that the capture head captures the target substance in the sample, the target substance captured by the capture head reacts with the reagent to obtain the product to be tested, and the detection mechanism is controlled to detect the product to be tested, so as to obtain the detection result of the sample in one detection.

25. The sample analyzer according to claim 24, characterized in that: The capture head comprises: A connecting portion, the connecting portion being used to connect with the driving mechanism; A capture portion, the capture portion is connected to the connection portion or is integrally formed, the capture portion forms a plurality of surface contact structures, and the surface area of ​​the capture head is 1 to 5 times the surface area of ​​a cylinder of the same length and the same width; A coating layer is also formed on the surface of the capture part, and the coating layer includes a first substance to be bound and / or a substance capable of binding to the first substance to be bound, and the first substance to be bound can bind to a target substance in the sample.

26. The sample analyzer according to claim 25, characterized in that: The length between one end of the connecting portion away from the capturing portion and one end of the capturing portion away from the connecting portion is 5 mm to 50 mm; and The width of the projection of the capturing portion along the axial direction of the capturing head is 2 mm to 8 mm.

27. The sample analyzer according to claim 26, characterized in that: The surface contact structure includes a spherical protrusion, the capturing portion includes a columnar body, at least part of the spherical protrusion is formed on the side of the columnar body; the diameter of the spherical protrusion is 0.2 mm to 2 mm.

28. A sample analyzer, characterized in that: include: The reagent kit storage area can hold at least one reagent kit. The invention comprises a sample receiving part, a capture head and a reagent receiving part, wherein the reagent receiving part contains a reagent; A sample dispensing mechanism, the sample dispensing mechanism is used to dispense the sample into the sample containing portion; A driving mechanism, the driving mechanism is used to drive the capture head to move; A detection mechanism, the detection mechanism is used to detect the product to be tested prepared based on the sample; A droplet removal mechanism, the droplet removal mechanism is used to remove droplets on the capture head; A controller, the controller is used to control the driving mechanism to place the capture head in the sample of the sample holding part so that the capture head captures the target substance in the sample; control the driving mechanism to take the capture head out of the sample, and control the driving mechanism to place the capture head in the reagent of the reagent holding part to react and obtain the product to be tested; control the detection mechanism to detect the product to be tested to obtain the detection result of the sample; The controller is also used to control the liquid droplet removal mechanism to remove at least part of the liquid droplets on the capture head after the capture head is removed from the sample and / or the reagent.

29. The sample analyzer according to claim 28, characterized in that: The liquid droplet removal mechanism is used to provide airflow to the capture head to remove the liquid droplets on the capture head.

30. A kit, characterized in that The reagent box is configured to include a sample containing portion and a reagent containing portion having an opening, and a capture head is also pre-installed on the reagent box; The capture head includes a capture portion, the capture portion forms a plurality of surface contact structures, and the surface area of ​​the capture head is 1 to 5 times the surface area of ​​a cylinder of the same length and the same width; The sample holding portion is used to hold a sample and is used for the capture head to be placed therein so that the capture portion can capture the target substance in the sample; The reagent containing part is filled with a reagent, and the capturing head is placed therein so that the target substance captured by the capturing head reacts with the reagent to obtain a product to be tested.

31. A control method for a sample analyzer, characterized in that: include: Controlling the sample dispensing mechanism to dispense the sample into the sample containing portion of the reagent box; Controlling the driving mechanism to place the capture head into the sample of the sample containing portion so that the capture head captures the target substance in the sample; Controlling the driving mechanism to remove the capture head from the sample; Controlling the driving mechanism to place the capture head in the reagent of the reagent containing part of the reagent kit to react and obtain a product to be tested; Controlling the detection mechanism to detect the product to be tested to obtain the detection result of the sample to be tested; Wherein, after the capture head is taken out from the sample and / or the reagent, at least part of the droplets on the capture head are removed.

32. The control method according to claim 31, characterized in that: After the capture head is taken out from the sample and / or the reagent, the driving mechanism is controlled to drive the capture head to rotate so as to remove at least part of the droplets on the capture head.

33. The control method according to claim 31, characterized in that: Before placing the capture head in the reagent of the reagent receiving portion of the reagent reagent cartridge, and / or after the capture head takes out and removes at least part of the droplets from the reagent, the method further comprises: Controlling the driving mechanism to place the capture head in a cleaning solution for cleaning; Controlling the driving mechanism to remove the capture head from the cleaning liquid; After the capture head is taken out of the cleaning liquid, at least part of the liquid droplets on the capture head are removed.

34. A sample analyzer, characterized in that: include: A reagent kit storage area, wherein at least one reagent kit can be placed in the reagent kit storage area, and the reagent kit includes a receiving portion and a capture head; A dispensing mechanism, the dispensing mechanism is used to dispense the sample into the containing part, the containing part is pre-installed with a reagent; or the containing part includes a sample containing part and a reagent containing part, the reagent containing part is pre-installed with a reagent, the dispensing mechanism is used to dispense the sample into the sample containing part and to dispense the reagent in the reagent containing part into the sample containing part; A driving mechanism, the driving mechanism is used to drive the capture head to move; A detection mechanism, the detection mechanism is used to detect the product to be tested prepared based on the sample; A controller is used to control the driving mechanism to place the capture head in the containing part including the sample and the reagent so that the capture head captures the target substance in the sample, and reacts in the containing part to obtain the product to be tested; and control the detection mechanism to detect the product to be tested to obtain the detection result of the sample.

35. The sample analyzer according to claim 34, characterized in that: The controller is further used to control the driving mechanism to drive the capturing head to rotate after the capturing head is taken out of the liquid in the containing part; or The sample analyzer also includes a droplet removal mechanism, which is used to remove the captured The controller is also used to control the droplet removal mechanism to remove at least part of the droplets on the capture head after the capture head is taken out of the liquid in the receiving portion.

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