Bubble candy capable of supporting vision health and preparation method of bubble candy

Abstract

The invention discloses bubble candies capable of supporting vision health and a preparation method of the bubble candies, and belongs to the technical field of food processing. The bubble candy comprises vitamin C, sodium ascorbate, lutein ester, zeaxanthin, astaxanthin, anthocyanin, beta carotene, semen cassiae, chrysanthemum, DL-malic acid, citric acid, sodium bicarbonate, sorbitol, xylitol, sucralose, stevioside, aspartame, passion fruit powder essence, magnesium stearate, sunset yellow aluminum lake, allura red aluminum lake and lemon yellow aluminum lake. The preparation method comprises the following steps: (1) weighing; (2) mixing; (3) tabletting; and (4) packaging. Starting from the goal of protecting eyesight health, vitamins, minerals and functional plant extract raw materials are taken as main materials, a carrier sorbitol with good compressibility, citric acid capable of improving bubble feeling, sodium bicarbonate and the like are taken as auxiliary materials, and according to a high-quality production process, the product dosage form is designed into the bubble tablet candy, so that the bubble tablet candy has better stability, and is suitable for popularization and application. The carrying is convenient.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of food processing, more particularly to a bubble candy supporting vision health and a preparation method thereof. BACKGROUND

[0002] With the acceleration of modern life pace, people's diet is irregular, and the problem of unbalanced nutrition intake occurs frequently. The demand for convenient and efficient nutrition supplements and health products is increasing. At the same time, in the current information and intelligent era, people's vision health faces serious social problems. The popularity of electronic screens increases the time people spend on electronic devices. Staring at the screen for a long time reduces the number of eye blinks, and the lack of tear secretion leads to eye dryness, stinging, and foreign body sensation. In addition, it can also cause sustained contraction of the ciliary muscle, thereby increasing the risk of myopia and ocular fundus lesions.

[0003] Vitamin C, as a water-soluble vitamin essential for the human body, has key physiological functions such as antioxidant, collagen synthesis promotion, and immune enhancement. Since the human body cannot synthesize it independently, it needs to rely on external intake and supplementation. Current vitamin C supplements have injection, capsule, tablet, and other dosage forms. Among them, effervescent tablets are favored by consumers due to their rich taste and fast absorption, but the water-soluble way is not convenient, which to some extent limits the use scenario. Under the trend of upgrading consumer demand, bubble candy that combines the sensory experience of bubbles and the function of vitamin C supplementation has become a popular development direction of new healthy snacks.

[0004] Currently, there are some bubble-type tablet candy products on the market. They usually use sodium bicarbonate and acidic ingredients such as citric acid to produce carbon dioxide gas, thereby forming a unique bubble texture in the mouth. However, existing products still have many shortcomings in formula design and production process. For example, some products release bubbles too quickly or have a short duration, resulting in poor sensory experience; vitamin C is easily affected by factors such as humidity, temperature, and metal ions during processing and storage, leading to degradation and poor stability; traditional tablet candies have a tight texture and slow dissolution, affecting the release and absorption efficiency of active ingredients. In addition, most products lack systematic optimization in terms of raw material compatibility, process control, and dosage form adaptability, making it difficult to balance functionality, stability, and interest.

[0005] Therefore, how to develop a new type of healthy bubble tablet candy that can precisely control bubble release, improve vitamin C, support vision health, stabilize nutritional active ingredients, and optimize dissolution characteristics is a problem that needs to be solved by those skilled in the art. SUMMARY

[0006] Therefore, the purpose of the present application is to provide a bubble candy supporting vision health and a preparation method thereof to solve the problems in the prior art.

[0007] In order to achieve the above object, the present application adopts the following technical solutions: A bubble candy supporting vision health comprises the following raw materials by weight: vitamin C 30.000 parts, vitamin C sodium 22.800 parts, lutein ester 5 parts, corn xanthophyll 1 part, astaxanthin 0.1 part, anthocyanin 1 part, beta carotene 0.1 part, cassia seed 1 part, chrysanthemum 1 part, DL-malic acid 15.000 parts, citric acid 75.000 parts, sodium bicarbonate 100.000 parts, sorbitol 240.095-240.595 parts, xylitol 40.800 parts, sucralose 0.500 parts, steviol glycoside 2.000-2.500 parts, aspartame 1.500 parts, passion fruit powder essence 2.500 parts, magnesium stearate 10.000 parts, sunset yellow aluminum lake 0.055 parts, temptation red aluminum lake 0.010 parts, and lemon yellow aluminum lake 0.040 parts.

[0008] Preferably, vitamin C 30.000 parts, vitamin C sodium 22.800 parts, lutein ester 5 parts, corn xanthophyll 1 part, astaxanthin 0.1 part, anthocyanin 1 part, beta carotene 0.1 part, cassia seed 1 part, chrysanthemum 1 part, DL-malic acid 15.000 parts, citric acid 75.000 parts, sodium bicarbonate 100.000 parts, sorbitol 240.095 parts, xylitol 40.800 parts, sucralose 0.500 parts, steviol glycoside 2.500 parts, aspartame 1.500 parts, passion fruit powder essence 2.500 parts, magnesium stearate 10.000 parts, sunset yellow aluminum lake 0.055 parts, temptation red aluminum lake 0.010 parts, and lemon yellow aluminum lake 0.040 parts.

[0009] Or, preferably, vitamin C 30.000 parts, vitamin C sodium 22.800 parts, lutein ester 5 parts, corn xanthophyll 1 part, astaxanthin 0.1 part, anthocyanin 1 part, beta carotene 0.1 part, cassia seed 1 part, chrysanthemum 1 part, DL-malic acid 15.000 parts, citric acid 75.000 parts, sodium bicarbonate 100.000 parts, sorbitol 240.395 parts, xylitol 40.800 parts, sucralose 0.500 parts, steviol glycoside 2.200 parts, aspartame 1.500 parts, passion fruit powder essence 2.500 parts, magnesium stearate 10.000 parts, sunset yellow aluminum lake 0.055 parts, temptation red aluminum lake 0.010 parts, and lemon yellow aluminum lake 0.040 parts.

[0010] Or, preferably: Vitamin C 30.000 parts, Vitamin C sodium 22.800 parts, lutein ester 5 parts, corn xanthophyll 1 part, astaxanthin 0.1 part, anthocyanin 1 part, beta carotene 0.1 part, cassia 1 part, chrysanthemum 1 part, DL-malic acid 15.000 parts, citric acid 75.000 parts, sodium bicarbonate 100.000 parts, sorbitol 240.595 parts, xylitol 40.800 parts, sucralose 0.500 parts, steviol glycosides 2.000 parts, aspartame 1.500 parts, passion fruit powder essence 2.500 parts, magnesium stearate 10.000 parts, sunset yellow aluminum lake 0.055 parts, allura red aluminum lake 0.010 parts and lemon yellow aluminum lake 0.040 parts.

[0011] In the present application, Vitamin C is provided by Vitamin C and Vitamin C sodium complex, which meets the human body's needs while providing good taste.

[0012] A preparation method of a bubble candy supporting eye health, specifically comprising the following steps: (1) weighing Each raw material is weighed according to the above weight fraction of the bubble candy supporting eye health, and is crushed and sieved; (2) mixing Sunset yellow aluminum lake, allura red aluminum lake, lemon yellow aluminum lake, sucralose and an equal amount of sodium bicarbonate are mixed in equal amounts, sieved after uniform mixing, and mixture A is obtained; Steviol glycosides, aspartame, passion fruit powder essence and an equal amount of sodium bicarbonate are mixed uniformly and sieved, and mixture B is obtained; Mixtures A, B, Vitamin C, Vitamin C sodium, lutein ester, corn xanthophyll, astaxanthin, anthocyanin, beta carotene, cassia, chrysanthemum, DL-malic acid, citric acid, sorbitol, xylitol, magnesium stearate and the remaining sodium bicarbonate are mixed uniformly to obtain mixture C; (3) tabletting Mixing C is compressed to obtain tablets; (4) packaging The compressed tablets are bottled through an automatic packaging line to obtain the bubble candy supporting eye health.

[0013] Further, in the above step (1), the crushing and sieving is specifically as follows: Vitamin C, Vitamin C sodium, lutein ester, corn xanthophyll, astaxanthin, anthocyanin, beta carotene, cassia, chrysanthemum, DL-malic acid and citric acid are crushed and sieved through an 80-mesh sieve, and sodium bicarbonate, sorbitol, xylitol, sucralose, steviol glycosides, aspartame, passion fruit powder essence, magnesium stearate, sunset yellow aluminum lake, allura red aluminum lake and lemon yellow aluminum lake are crushed and sieved through a 40-mesh sieve.

[0014] Further, in the step (2), the number of times of the equal increment mixing is 3.

[0015] Further, in the step (2), the mesh number of the screen is 60 meshes.

[0016] Further, in the step (3), the pressure of the pressing is 80-120 N.

[0017] Further, in the step (3), the friability of the tablet is ≤0.5%.

[0018] Compared with the prior art, the beneficial effects of the present application are as follows: The present application takes protecting vision health as the goal, takes vitamins, minerals and functional plant raw materials as the main materials, and takes sorbitol with good compressibility, citric acid and sodium bicarbonate for improving bubble feeling as the auxiliary materials, and designs the product dosage form into a bubble compressed tablet candy according to a high-quality production process, so that the product has better stability, is convenient to carry, and is more interesting than traditional compressed tablet candies. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 Effect of FEH compound mass concentration (ug / mL) on APRE-19 cell viability (Note: *** indicates high significance difference, P <0.001); Figure 2 Effect of MGO concentration on APRE-19 cell viability; Figure 3 Effect of FEH compound mass concentration (ug / mL) on MGO-induced APRE-19 cell viability (Note: * indicates significant difference, P <0.05; ** indicates extremely significant difference, P <0.01; *** indicates high significance difference, P <0.001); Figure 4 Effect of FEH compound mass concentration on MGO-induced APRE-19 cell ROS level (Note: A: control group; B: MGO group; C: MGO+0.01 μg / mL FEH group; D: MGO+0.10 μg / mL FEH group; E: MGO+1.00 μg / mL FEH group; F: MGO+10.00 μg / mL FEH group); Figure 5 Effect of FEH compound mass concentration on MGO-induced APRE-19 cell NO content (A), SOD activity (B) and GSH-Px activity (C) (Note: * indicates significant difference, P <0.05; ** indicates extremely significant difference,P <0.01). DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present application will be described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0021] Embodiment 1 The bubble candy for supporting eyesight health comprises the following raw materials by weight: vitamin C 30.000 mg, vitamin C sodium 22.800 mg, lutein ester 5 mg, corn xanthophyll 1 mg, astaxanthin 0.1 mg, anthocyanin 1 mg, beta carotene 0.1 mg, cassia seed 1 mg, chrysanthemum 1 mg, DL-malic acid 15.000 mg, citric acid 75.000 mg, sodium bicarbonate 100.000 mg, sorbitol 240.095 mg, xylitol 40.800 mg, sucralose 0.500 mg, steviol glycosides 2.500 mg, aspartame 1.500 mg, passion fruit powder essence 2.500 mg, magnesium stearate 10.000 mg, sunset yellow aluminum lake 0.055 mg, allura red aluminum lake 0.010 mg, and lemon yellow aluminum lake 0.040 mg; The preparation method of the above bubble candy specifically comprises the following steps: (1) weighing The raw materials are weighed according to the weight of the above bubble candy for supporting eyesight health. The vitamin C, vitamin C sodium, lutein ester, corn xanthophyll, astaxanthin, anthocyanin, beta carotene, cassia seed, chrysanthemum, DL-malic acid, and citric acid are crushed and sieved through an 80-mesh sieve. The sodium bicarbonate, sorbitol, xylitol, sucralose, steviol glycosides, aspartame, passion fruit powder essence, magnesium stearate, sunset yellow aluminum lake, allura red aluminum lake, and lemon yellow aluminum lake are crushed and sieved through a 40-mesh sieve. (2) mixing The sunset yellow aluminum lake, allura red aluminum lake, lemon yellow aluminum lake, and sucralose are mixed with an equal amount of sodium bicarbonate in an equal incremental manner for three times. After being uniformly mixed, the mixture is sieved through a 60-mesh sieve to obtain a mixture A. The steviol glycosides, aspartame, and passion fruit powder essence are uniformly mixed with an equal amount of sodium bicarbonate and sieved through a 60-mesh sieve to obtain a mixture B. The mixture A, mixture B, vitamin C, vitamin C sodium, lutein ester, corn xanthophyll, astaxanthin, anthocyanin, beta carotene, cassia seed, chrysanthemum, DL-malic acid, citric acid, sorbitol, xylitol, magnesium stearate, and the remaining sodium bicarbonate are uniformly mixed to obtain a mixture C. (3) tabletting The mixture C is pressed at a pressure of 80 N to obtain tablets with friability ≤ 0.5%; Tablet weight: 550 mg (± 4%), check the average tablet weight every 15 min; check the single tablet weight every 30 min, take 10 tablets each time, check the appearance, tablet weight difference, etc. (4) Packaging The pressed tablets are bottled through an automatic packaging line to obtain the bubble candy for supporting eye health. (5) Inspection and submission After packaging, please check and take samples for submission by the QA personnel.

[0022] Example 2 The bubble candy for supporting eye health comprises the following raw materials by weight: vitamin C 30.000 mg, vitamin C sodium 22.800 mg, lutein ester 5 mg, zeaxanthin 1 mg, astaxanthin 0.1 mg, anthocyanin 1 mg, beta carotene 0.1 mg, cassia seed 1 mg, chrysanthemum 1 mg, DL-malic acid 15.000 mg, citric acid 75.000 mg, sodium bicarbonate 100.000 mg, sorbitol 240.395 mg, xylitol 40.800 mg, sucralose 0.500 mg, steviol glycosides 2.200 mg, aspartame 1.500 mg, passion fruit powder essence 2.500 mg, magnesium stearate 10.000 mg, sunset yellow aluminum lake 0.055 mg, allura red aluminum lake 0.010 mg, and lemon yellow aluminum lake 0.040 mg. The preparation method of the above bubble candy specifically comprises the following steps: (1) Weighing The raw materials are weighed according to the weight of the above bubble candy for supporting eye health. The vitamin C, vitamin C sodium, lutein ester, zeaxanthin, astaxanthin, anthocyanin, beta carotene, cassia seed, chrysanthemum, DL-malic acid, and citric acid are crushed and passed through an 80-mesh sieve. The sodium bicarbonate, sorbitol, xylitol, sucralose, steviol glycosides, aspartame, passion fruit powder essence, magnesium stearate, sunset yellow aluminum lake, allura red aluminum lake, and lemon yellow aluminum lake are crushed and passed through a 40-mesh sieve. (2) Mixing The sunset yellow aluminum lake, allura red aluminum lake, lemon yellow aluminum lake, sucralose, and an equal amount of sodium bicarbonate are mixed in equal increments three times, and then passed through a 60-mesh sieve to obtain mixture A. The steviol glycosides, aspartame, passion fruit powder essence, and an equal amount of sodium bicarbonate are mixed uniformly and then passed through a 60-mesh sieve to obtain mixture B. Mixing mixture A, mixture B, vitamin C, sodium ascorbate, lutein ester, zeaxanthin, astaxanthin, anthocyanin, beta carotene, cassia obtusifolia, chrysanthemum, DL-malic acid, citric acid, sorbitol, xylitol, magnesium stearate and the remaining sodium bicarbonate to obtain mixture C; (3) tabletting Tabletting mixture C with a pressure of 100N to obtain tablets with a friability of ≤0.5%; Tablet weight: 550mg (±4%), check the average tablet weight every 15min, check the single tablet weight every 30min, take 10 tablets each time, check the appearance, tablet weight difference and other items; (4) packaging The tableted tablets are packaged by automatic packaging line to obtain the bubble candy for supporting eye health. (5) inspection, submission After packaging, please check, and the QA personnel take samples for inspection.

[0023] Example 3 The bubble candy for supporting eye health comprises the following raw materials by weight: vitamin C 30.000mg, sodium ascorbate 22.800mg, lutein ester 5mg, zeaxanthin 1mg, astaxanthin 0.1mg, anthocyanin 1mg, beta carotene 0.1mg, cassia obtusifolia 1mg, chrysanthemum 1mg, DL-malic acid 15.000mg, citric acid 75.000mg, sodium bicarbonate 100.000mg, sorbitol 240.595mg, xylitol 40.800mg, sucralose 0.500mg, steviol glycosides 2.000mg, aspartame 1.500mg, passion fruit powder flavor 2.500mg, magnesium stearate 10.000mg, sunset yellow aluminum lake 0.055mg, allura red aluminum lake 0.010mg and lemon yellow aluminum lake 0.040mg. The preparation method of the above bubble candy specifically comprises the following steps: (1) weighing Weigh each raw material according to the weight of the above bubble candy for supporting eye health, and sieve the vitamin C, sodium ascorbate, lutein ester, zeaxanthin, astaxanthin, anthocyanin, beta carotene, cassia obtusifolia, chrysanthemum, DL-malic acid and citric acid through an 80-mesh sieve, and sieve the sodium bicarbonate, sorbitol, xylitol, sucralose, steviol glycosides, aspartame, passion fruit powder flavor, magnesium stearate, sunset yellow aluminum lake, allura red aluminum lake and lemon yellow aluminum lake through a 40-mesh sieve; (2) mixing Mix sunset yellow aluminum lake, allura red aluminum lake, lemon yellow aluminum lake, sucralose and an equal amount of sodium bicarbonate in equal increments 3 times, sieve the mixture through a 60-mesh sieve after mixing evenly to obtain mixture A; Mixing the steviol glycosides, aspartame, passion fruit powder essence with equal amount of sodium bicarbonate uniformly, and then passing through a 60 mesh sieve to obtain mixture B; Mixing mixture A, mixture B, vitamin C, sodium ascorbate, lutein ester, zeaxanthin, astaxanthin, anthocyanin, beta carotene, cassia seed, chrysanthemum, DL-malic acid, citric acid, sorbitol, xylitol, magnesium stearate and the remaining sodium bicarbonate uniformly to obtain mixture C; (3) tabletting Tabletting mixture C at a pressure of 120 N to obtain tablets with a friability of ≤0.5%; Tablet weight: 550 mg (±4%), check the average tablet weight every 15 min; check the single tablet weight every 30 min, take 10 tablets each time, and check the appearance, tablet weight difference and other items; (4) packaging The tabletted tablets are packaged by an automatic packaging line to obtain the eye health supporting bubble candy; (5) inspection and submission After packaging, please inspect, and the QA personnel take samples for submission.

[0024] Performance test 1. Pilot production inspection Take the eye health supporting bubble candy (specification: 0.55 g / tablet) prepared in Examples 1-3 respectively, and test their sensory index, physicochemical index and microbial index (inspection basis: GB 24154), and the results are shown in Table 1.

[0025] Table 1 Pilot production inspection report of bubble candy of Examples 1-3

[0026] As can be seen from Table 1, the sensory index, physicochemical index and microbial index of the eye health supporting bubble candy of Examples 1-3 are all qualified for inspection.

[0027] 2. Vision protection effect verification Take the eye health supporting bubble candy (hereinafter also referred to as "FEH compound") prepared in Example 2, and verify its protective effect on the oxidative stress damage of human retinal pigment epithelial cells (Retinal pigment epithelium, APRE-19) induced by methylglyoxal (MGO).

[0028] 1. Verification method 1.1 APRE-19 cell culture After the APRE-19 cells were taken out by liquid nitrogen tank, they were transferred to DMEM / F12 medium containing 10% fetal bovine serum, 100 U / mL penicillin and 100 g / L streptomycin, and were placed in a 37°C, 5% CO2 constant temperature cell incubator for culture, and cell recovery was performed. The culture solution was replaced every day until the APRE-19 cells grew to about 90% cell confluence, and they were passaged, and the logarithmic phase growth cells were selected for subsequent experiments.

[0029] 1.2 Effect of MGO and FEH complex on the viability of APRE-19 cells The logarithmic growth phase APRE-19 cells were divided into a control group, an MGO group and an FEH complex group, and were inoculated in a 96-well plate at a standard of 1×10 4 cells per well. After 24 h of inoculation, the complete culture medium was discarded. The control group APRE-19 cells were added with serum-free medium, the MGO group APRE-19 cells were added with 0.25, 0.50, 1, 2, 4 mmol / L MGO solution, and the FEH complex group APRE-19 cells were added with 0.01, 0.1, 1, 10, 100 μg / mL FEH complex solution, and were incubated for 18 h.

[0030] 1.3 Effect of FEH complex on the viability of MGO-induced APRE-19 cells The logarithmic growth phase APRE-19 cells were divided into a control group, an MGO group, an MGO+0.01 μg / mL FEH group, an MGO+0.10 μg / mL FEH group, an MGO+1.00 μg / mL FEH group, an MGO+10.00 μg / mL FEH group, and were inoculated in a 96-well plate at a standard of 1×10 4 cells per well. After 24 h of inoculation, the complete culture medium was discarded. The control group and the MGO group APRE-19 cells were added with serum-free medium, and the MGO+different mass concentration FEH group APRE-19 cells were added with 0.01, 0.10, 1.00, 10.00 μg / mL FEH complex solution. After 6 h of incubation, the MGO group and the MGO+different mass concentration FEH group were continuously added with MGO, so that the final concentration of MGO was 0.08 mmol / L, and were incubated for 18 h.

[0031] 1.4 CCK-8 detection of cell viability The logarithmic growth phase APRE-19 cells were divided into groups according to 7.2 and 7.3, and were inoculated in a 96-well plate at a standard of 1×10 4 cells per well. After 24 h of culture, 100 μL of CCK-8 reagent (CCK-8: serum-free medium = 1:9) was added, and was incubated for 3 h. The absorbance value at 450 nm was determined, and the cell viability was calculated.

[0032] 1.5 Detection of cellular oxidative stress level 1.5.1 ROS content determination Reference to the method of Gan Jing (Gan Jing. Apigenin alleviates aflatoxin B1-induced apoptosis in human embryonic kidney cells by regulating oxidative phosphorylation and caspase signaling pathway[D]. Northwest A&F University, 2017.) and make slight adjustments. After the APRE-19 cells were washed with phosphate buffer (PBS), 10 μmol / L of active oxygen fluorescent probe (2’7-dichlorofluorescin diacetate, DCFH-DA) was added, and the cells were incubated at 37°C in the dark for 30 min. After washing with serum-free cell culture medium to remove excess probe, the fluorescence microscope parameters were set to excitation wavelength 485 nm and emission wavelength 528 nm, and the ROS fluorescence intensity was observed.

[0033] 1.5.2 NO content, GSH-Px and SOD activity determination According to the kit instructions, the SOD activity of APRE-19 cell lysate was determined by hydroxylamine method, the NO content was determined by ELISE method, and the GSH-Px activity was determined by colorimetric method.

[0034] 2 Verification results 2.1 Effect of bubble candy FEH complex on APRE-19 cell viability According to the APRE-19 cell viability, the safe quality concentration of FEH complex was screened, and the results are shown in Figure 1 0, 0.01, 0.10, 1.00, 10.00 μg / mL FEH complex had no significant effect on the survival rate of APRE-19 cells ( P >0.05), indicating that 0~10.00 μg / mL FEH had no toxic effect on APRE-19 cells. When 100.00 μg / mL FEH complex was used to treat APRE-19 cells, the survival rate of APRE-19 cells decreased by 15.43%, which was highly significant ( P <0.001), and the toxicity of APRE-19 cells was obvious. Therefore, 0.01, 0.10, 1.00, 10.00 μg / mL were selected for cell experiments.

[0035] 2.2 Effect of MGO on APRE-19 cell viability According to the APRE-19 cell viability, the MGO induction concentration was screened, and the results are shown in Figure 2As shown, the inhibition rate of APRE-19 cell viability increased significantly with increasing MGO concentration. Curve fitting using GraphPad Prism 6.0 software yielded a half-maximal inhibitory concentration (IC50) of (0.77 ± 0.07) mmol / L for MGO treatment of APRE-19 cells. Considering specific experimental procedures, a concentration of 0.80 mmol / L of MGO was selected to induce APRE-19 cells and establish a cell damage model.

[0036] 2.3 Effect of bubble candy FEH complex on MGO-induced APRE-19 cell viability MGO-induced APRE-19 cell viability was observed after treatment with 0.01, 0.10, 1.00, and 10.00 μg / mL FEH complex. Figure 3 Compared with the MGO group, all FEH concentration groups significantly improved APRE-19 cell viability. The 0.01 μg / mL concentration showed the most significant improvement. P <0.05), and a mass concentration of 10 μg / mL was extremely significant ( P <0.01), the increase in mass concentration at 0.10 and 1.00 μg / mL was highly significant ( P The levels of FEH complex were <0.001%, increasing by 21.23% and 23.03% respectively. This indicates that the FEH complex has an effective protective effect against oxidative stress-induced damage to APRE-19 cell viability.

[0037] 2.4 Effect of bubble candy FEH complex on MGO-induced ROS production in APRE-19 cells The result is Figure 4 As shown in the figure. Compared with the control group, MGO intervention significantly increased the oxidative stress level of ARPE-19 cells, and the fluorescence intensity of ROS in the cells increased by 2.89 times compared with the control group, which was statistically significant. P <0.05). Compared with the MGO group, except for the FEH complex group (1.00 μg / mL), all other groups significantly reduced the fluorescence intensity of ROS in ARPE-19 cells ( P The fluorescence intensity of ROS in cells was <0.05 μg / mL in the FEH complex groups (0.01, 0.10, and 10.00 μg / mL) decreased to 1.02, 1.28, and 2.37 times that of the MGO group, respectively. This indicates that a certain concentration of the FEH complex can inhibit the generation of ROS in vivo, thereby achieving a protective effect against oxidative stress in ocular cells.

[0038] 2.5 Effects of the FEH complex from bubble candy on NO content, GSH-Px and SOD activity in MGO-induced APRE-19 cells To further investigate the effect of the FEH complex on the MGO-induced antioxidant capacity of APRE-19 cells, the NO content, GSH-Px, and SOD activity in APRE-19 cells were measured. Figure 5 As is known in Chinese A, compared with the control group, MGO induction can significantly promote NO production in ARPE-19 cells ( P <0.01), NO production was 2.53 times that of the normal group. Compared with the MGO model group, except for the 1.00 μg / mL FEH complex, 0.01, 0.10, and 10.00 μg / mL concentrations all reduced NO production in ARPE-19 cells, specifically by 13.24, 15.23, and 16.24 μmol / gprot, respectively, which were statistically significant. P <0.05). This indicates that a certain dose and concentration of the FEH complex can inhibit the production of NO in vivo. (From...) Figure 5 As is known in Chinese medicine, compared with the control group, MGO intervention can significantly reduce the activity of SOD in ARPE-19 cells. P <0.01); Compared with the MGO group, the FEH complex dose-dependently increased the SOD activity of ARPE-19 cells, with treatments of 0.10, 1.00, and 10.00 μg / mL FEH complex significantly increasing SOD activity in ARPE-19 cells by 5.23 (μg / mL, respectively). P <0.05), 6.01 ( P <0.01), 7.43 U / mg ( P <0.01). By Figure 5 As is known in China, compared with the control group, MGO intervention can significantly inhibit the activity of GSH-Px in ARPE-19 cells. P <0.05), compared with the MGO group, the FEH complex dose-dependently increased the activity of GSH-Px in ARPE-19 cells. Specifically, treatment with 1.00 and 10.00 μg / mL FEH complex significantly increased the activity of GSH-Px in ARPE-19 cells by 13.32 and 22.42 U / mg, respectively. P <0.05).

[0039] 3. Conclusion The results showed that the FEH complex in the bubble candy of this invention can maintain the biological functional integrity of ARPE-19 cells under oxidative stress damage, fully exerting its protective effect on ocular cells. Compared with the ocular protective effects of other natural complexes, the FEH complex exhibited a stronger ability to enhance SOD and GSH-Px activity.

[0040] The foregoing description of the disclosed embodiments enables a person skilled in the art to make or use the application. Modifications of these embodiments will occur to persons of skill in the art, and that the appended claims are intended to cover all such modifications that do not depart from the true spirit and scope of the application. Therefore, the application is not limited to the embodiments shown but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A type of bubble candy that supports eye health, characterized in that, The ingredients include the following parts by weight: Vitamin C 30,000 parts, Sodium Vitamin C 22,800 parts, Lutein Ester 5 parts, Zeaxanthin 1 part, Astaxanthin 0.1 part, Anthocyanin 1 part, β-carotene 0.1 part, Cassia Seed 1 part, Chrysanthemum 1 part, DL-Malic Acid 15,000 parts, Citric Acid 75,000 parts, Sodium Bicarbonate 100,000 parts, Sorbitol 240.095-240.595 parts, Xylitol 40.800 parts, Sucralose 0.500 parts, Stevioside 2,000-2.500 parts, Aspartame 1,500 parts, Passion Fruit Powder Flavor 2,500 parts, Magnesium Stearate 10,000 parts, Sunset Yellow Aluminum Lake 0.055 parts, Allura Red Aluminum Lake 0.010 parts, and Tartrazine Aluminum Lake 0.040 parts.

2. The bubble candy that supports vision health according to claim 1, characterized in that, The ingredients include the following parts by weight: Vitamin C 30,000 parts, Sodium Vitamin C 22,800 parts, Lutein Ester 5 parts, Zeaxanthin 1 part, Astaxanthin 0.1 part, Anthocyanin 1 part, Beta-carotene 0.1 part, Cassia Seed 1 part, Chrysanthemum 1 part, DL-Malic Acid 15,000 parts, Citric Acid 75,000 parts, Sodium Bicarbonate 100,000 parts, Sorbitol 240,095 parts, Xylitol 40,800 parts, Sucralose 0.500 parts, Stevioside 2,500 parts, Aspartame 1,500 parts, Passion Fruit Powder Flavor 2,500 parts, Magnesium Stearate 10,000 parts, Sunset Yellow Aluminum Lake 0.055 parts, Allura Red Aluminum Lake 0.010 parts, and Tartrazine Aluminum Lake 0.040 parts.

3. The bubble candy that supports vision health according to claim 1, characterized in that, The ingredients include the following parts by weight: Vitamin C 30,000 parts, Sodium Vitamin C 22,800 parts, Lutein Ester 5 parts, Zeaxanthin 1 part, Astaxanthin 0.1 part, Anthocyanin 1 part, Beta-carotene 0.1 part, Cassia Seed 1 part, Chrysanthemum 1 part, DL-Malic Acid 15,000 parts, Citric Acid 75,000 parts, Sodium Bicarbonate 100,000 parts, Sorbitol 240,395 parts, Xylitol 40,800 parts, Sucralose 0.500 parts, Stevioside 2,200 parts, Aspartame 1,500 parts, Passion Fruit Powder Flavor 2,500 parts, Magnesium Stearate 10,000 parts, Sunset Yellow Aluminum Lake 0.055 parts, Allura Red Aluminum Lake 0.010 parts, and Tartrazine Aluminum Lake 0.040 parts.

4. The bubble candy that supports vision health according to claim 1, characterized in that, The ingredients include the following parts by weight: Vitamin C 30,000 parts, Sodium Vitamin C 22,800 parts, Lutein Ester 5 parts, Zeaxanthin 1 part, Astaxanthin 0.1 part, Anthocyanin 1 part, Beta-carotene 0.1 part, Cassia Seed 1 part, Chrysanthemum 1 part, DL-Malic Acid 15,000 parts, Citric Acid 75,000 parts, Sodium Bicarbonate 100,000 parts, Sorbitol 240,595 parts, Xylitol 40,800 parts, Sucralose 0.500 parts, Stevioside 2,000 parts, Aspartame 1,500 parts, Passion Fruit Powder Flavor 2,500 parts, Magnesium Stearate 10,000 parts, Sunset Yellow Aluminum Lake 0.055 parts, Allura Red Aluminum Lake 0.010 parts, and Tartrazine Aluminum Lake 0.040 parts.

5. A method for preparing a bubble candy that supports vision health, characterized in that, Specifically, the following steps are included: (1) Weighing Weigh each raw material according to the weight proportions of the bubble candy that supports vision health according to any one of claims 1-4, and crush and sieve them; (2) Mixing Sunset Yellow aluminum lake, Allure Red aluminum lake, Lemon Yellow aluminum lake, sucralose and sodium bicarbonate were mixed in equal increments, and after being thoroughly mixed, the mixture was sieved to obtain mixture A. Steviol glycosides, aspartame, passion fruit powder flavoring and an equal amount of sodium bicarbonate were mixed evenly and then sieved to obtain mixture B; Mixture A, mixture B, vitamin C, sodium vitamin C, lutein ester, zeaxanthin, astaxanthin, anthocyanins, β-carotene, cassia seed, chrysanthemum, DL-malic acid, citric acid, sorbitol, xylitol, magnesium stearate, and the remaining sodium bicarbonate are mixed evenly to obtain mixture C. (3) Tableting The mixture C is compressed to obtain tablets; (4) Packaging The compressed tablets are bottled via an automated packaging line to obtain the eye health-supporting bubble candy.

6. The method for preparing a bubble candy that supports vision health according to claim 5, characterized in that, In step (1), the pulverization and sieving specifically involves: pulverizing vitamin C, sodium vitamin C, lutein ester, zeaxanthin, astaxanthin, anthocyanins, β-carotene, cassia seed, chrysanthemum, DL-malic acid, and citric acid and then sieving them through an 80-mesh sieve; and pulverizing sodium bicarbonate, sorbitol, xylitol, sucralose, steviol glycosides, aspartame, passion fruit powder flavoring, magnesium stearate, sunset yellow aluminum lake, allura red aluminum lake, and tartrazine aluminum starch and then sieving them through a 40-mesh sieve.

7. The method for preparing a bubble candy that supports vision health according to claim 5, characterized in that, In step (2), the number of times the mixture is mixed in equal increments is 3.

8. The method for preparing a bubble candy that supports vision health according to claim 5, characterized in that, In step (2), the sieve mesh number is 60 mesh.

9. The method for preparing a bubble candy that supports vision health according to claim 5, characterized in that, In step (3), the pressing pressure is 80-120N.

10. The method for preparing a bubble candy that supports vision health according to claim 5, characterized in that, In step (3), the friability of the tablet is ≤0.5%.

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