Feed additive for improving intestinal health of rabbits and application thereof

Abstract

The invention relates to a feed additive for improving intestinal health of rabbits and application thereof, the feed additive is composed of bacillus licheniformis and saccharomyces cerevisiae in a mass ratio of 1: 2, the viable count of the bacillus licheniformis is greater than or equal to 5 * 10 < 7 > CFU / mL, and the viable count of the saccharomyces cerevisiae is greater than or equal to 3 * 10 < 7 > CFU / mL. Rabbit breeding tests show that the bacillus licheniformis powder and the saccharomyces cerevisiae have a synergistic effect, and the survival rate and the production performance of rabbits can be improved by improving the intestinal health condition of the rabbits.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of feed additives, in particular to a feed additive for improving the intestinal health of rabbits and application thereof. BACKGROUND

[0002] Rabbit meat is popular among consumers due to its high protein, low fat, and low cholesterol content. The demand for rabbit meat has gradually increased with the popularization of healthy eating concepts, especially in some areas where it has become a specialty dish or part of traditional cuisine. Rabbit breeding attracts breeders due to its low investment, high return rate, and short breeding cycle. Rabbit breeding is mainly small and medium-sized, and to some extent, it is decentralized, with varying levels of technical and management skills. Poor management in some rabbit farms can lead to gastrointestinal diseases such as diarrhea and enterogastritis, affecting breeding efficiency. The high incidence of rabbit gastrointestinal diseases is mainly due to the rabbit's unique digestive system, particularly the well-developed cecum. Grass contains a large amount of cellulose, which is difficult for humans and many other animals to digest. However, the rabbit's cecum has a large microbial community that effectively breaks down cellulose and other complex carbohydrates, converting them into nutrients that the rabbit can absorb and utilize. Maintaining a balanced and stable microbial community in the rabbit's cecum is crucial in preventing the onset of gastrointestinal diseases.

[0003] Bacillus licheniformis is a probiotic with excellent properties such as heat resistance, acid resistance, rich enzyme system, high enzyme production, and safety. It can adjust intestinal flora imbalance to achieve therapeutic purposes, promote the production of antibacterial active substances in the body, kill pathogenic bacteria, produce anti-active substances, and have a unique biological oxygen scavenging mechanism to inhibit the growth and reproduction of pathogenic bacteria. Bacillus licheniformis is a spore-forming bacterium that is highly resistant to acid and can pass through the acidic environment of the animal's stomach to enter the intestine, inhibit the growth and reproduction of intestinal pathogenic bacteria, and maintain a dynamic balance of intestinal microorganisms, thereby treating intestinal infections. In addition, it can also help treat indigestion by increasing the number of probiotic bacteria in the intestine and promoting food decomposition and absorption.

[0004] Saccharomyces cerevisiae can improve intestinal health, consume oxygen in the gastrointestinal tract, help create an anaerobic environment, and promote the reproduction of beneficial bacteria such as lactic acid bacteria, thereby maintaining the microecological balance in the animal's digestive tract. Saccharomyces cerevisiae also has the function of promoting digestion and absorption. Yeast is rich in various nutrients such as protein, B vitamins, trace elements, and various digestive enzymes, which promote the digestion and absorption of nutrients in feed by animals. Components such as yeast polysaccharides, functional polypeptides, and nucleotides help improve animal immunity and enhance disease resistance. Saccharomyces cerevisiae can be used as a mycotoxin adsorbent to reduce the harm of mycotoxins in feed to animals and ensure animal health.

[0005] At present, the common feed additives for improving the intestinal health of rabbits on the market are mainly antibiotics, and the product functions are single, which has certain influence on the health and later growth of rabbits, and there are few safe, healthy and effective preventive products used in the rabbit breeding process. There is no related report on the use of bacillus licheniformis and saccharomyces cerevisiae as feed additives for improving the intestinal health of rabbits. SUMMARY

[0006] The application provides a feed additive for improving the intestinal health of rabbits and application thereof, which is prepared by combining bacillus licheniformis and saccharomyces cerevisiae with rabbit feed to form granular feed, so as to solve the problems of gastrointestinal diseases such as diarrhea of rabbits, reduce the death rate of rabbits, promote the growth and development of rabbits, shorten the time of taking out of the market and improve the economic benefits of rabbit breeders.

[0007] In order to achieve the purpose of the application, in the first aspect, the application provides a feed additive for improving the intestinal health of rabbits, which is composed of bacillus licheniformis and saccharomyces cerevisiae, and the mass ratio of the bacillus licheniformis and the saccharomyces cerevisiae is 1:2, wherein:

[0008] The viable cell count of the bacillus licheniformis is ≥5×10 7 CFU / mL, preferably 6×10 7 CFU / mL;

[0009] The viable cell count of the saccharomyces cerevisiae is ≥3×10 7 CFU / mL, preferably 4×10 7 CFU / mL.

[0010] In the second aspect, the application provides application of the feed additive in preparing granular feed for reducing gastrointestinal diseases of rabbits, reducing the death rate of rabbits or improving the growth speed of rabbits.

[0011] The application further provides application of the feed additive in preparing granular feed for reducing gastrointestinal diseases of rabbits.

[0012] The granular feed comprises, by weight, 30-35 parts of alfalfa grass powder, 10-15 parts of grass family green hay grass powder, 22-25 parts of corn powder, 13-15 parts of cake and meal feed, 12-14 parts of bran, 1-1.5 parts of light calcium carbonate, 0.7-1 part of potassium dihydrogen phosphate, 1-1.5 parts of trace element premix, 1-1.5 parts of vitamin premix and 0.3-0.5 parts of salt.

[0013] The alfalfa grass powder has a crude protein content of ≥14% and a particle size of ≤0.3 cm.

[0014] The grass powder of the Poaceae green hay is selected from at least one of oat grass, ryegrass, wheat, barley and timothy grass, and the particle size of the grass powder of the Poaceae green hay is less than or equal to 0.3 cm.

[0015] The cake meal type feed is selected from at least one of soybean meal, rapeseed meal and cottonseed meal.

[0016] The microelement premix includes one or more of magnesium, sulfur, iron, copper, zinc, selenium, manganese, iodine, cobalt, chromium and molybdenum.

[0017] The vitamin premix is composed of vitamin A, vitamin D3, vitamin K3, vitamin E, vitamin B1, vitamin B2, vitamin B6, vitamin B12, nicotinamide, folic acid, calcium pantothenate and biotin.

[0018] In a third aspect, the present application provides application of the feed additive in rabbit breeding.

[0019] The feed additive is added in the rabbit feed in an amount of 150-500 kg / t.

[0020] In a fourth aspect, the present application provides a preparation method of the feed additive, which comprises: mixing Bacillus licheniformis powder and Saccharomyces cerevisiae powder with 35-37 DEG C pure water uniformly to obtain the feed additive, and diluting the feed additive according to the number of viable bacteria contained in the Bacillus licheniformis powder and the Saccharomyces cerevisiae powder during preparation.

[0021] The technical scheme of the present application has at least the following beneficial technical effects:

[0022] 1. The rabbit pellet feed is nutritionally balanced, and can provide comprehensive and rich nutrients for weaned young rabbits and adult rabbits, avoiding diseases caused by nutrient deficiency.

[0023] 2. The strains selected in the present application are acid-tolerant probiotics, which can avoid killing of viable bacteria in the granulation process, have good ability to pass through the gastric acid environment and colonize in the intestinal tract, and fully exert the regulating effect of the strains.

[0024] 3. Rabbit breeding tests show that the Bacillus licheniformis powder and the Saccharomyces cerevisiae have a synergistic effect, which can improve the intestinal health of rabbits and thus improve the survival rate and production performance of rabbits. BRIEF DESCRIPTION OF DRAWINGS

[0025] Figure 1 It is a live Bacillus licheniformis microscopic counting chart in Example 1 of the present application

[0026] Figure 2 It is a live Saccharomyces cerevisiae microscopic counting chart in Example 1 of the present application DETAILED DESCRIPTION

[0027] The present application is illustrated by the following examples, which should not be construed as limiting the application. If not specified, the technical means used in the examples are conventional means known to those skilled in the art, and the raw materials used are commercially available.

[0028] Example 1: Preparation of the feed additive described in the present application

[0029] 1. Composition

[0030] Bacillus licheniformis powder 100 g

[0031] Saccharomyces cerevisiae powder 200 g

[0032] 2. Preparation method

[0033] The Bacillus licheniformis powder and the Saccharomyces cerevisiae powder are mixed with 35-37°C pure water in a mass ratio of 1:2 to prepare the feed additive. During preparation, the Bacillus licheniformis powder and the Saccharomyces cerevisiae powder are diluted according to the number of viable bacteria contained therein. The number of viable bacteria of the prepared Bacillus licheniformis is ≥5x10 7 CFU / mL, and the number of viable bacteria of the Saccharomyces cerevisiae is ≥3x10 7 CFU / mL. The Bacillus licheniformis powder and the Saccharomyces cerevisiae powder can be commercially available.

[0034] The number of viable bacteria in the feed additive is detected by the method described in GB / T 34224-2017 Functional Microorganisms in Biological Products.

[0035] 3. Test results:

[0036] Example 1: The number of viable bacteria of Bacillus licheniformis is 6x10 7 CFU / mL, and the number of viable bacteria of Saccharomyces cerevisiae is 4x

[0037] 10 7 CFU / mL.

[0038] The amount of the feed additive prepared in this example added to the feed for rabbits is 100-150 kg / ton.

[0039] Example 2: Preparation of the feed additive described in the present application to prepare rabbit pellet feed

[0040] The composition of the rabbit feed is as follows: alfalfa powder 32%, oat grass powder 14%, corn powder 25%, soybean meal 8%, cottonseed meal 5%, bran 12%, light calcium carbonate 1%, potassium dihydrogen phosphate 0.7%, trace element premix 1%, vitamin premix 1%, and salt 0.3%.

[0041] The alfalfa grass powder has a crude protein content of 14% or more and a particle size of 0.3 cm or less.

[0042] The oat grass powder has a particle size of 0.3 cm or less.

[0043] The corn powder, soybean meal, cottonseed meal, bran, light calcium carbonate and potassium dihydrogen phosphate need to be in a powder form without caking and can pass through an 80-mesh sieve.

[0044] The trace element premix is composed of magnesium, sulfur, iron, copper, zinc, selenium, manganese, iodine, cobalt, chromium and molybdenum.

[0045] The vitamin premix is composed of vitamin A, vitamin D3, vitamin K3, vitamin E, vitamin B1, vitamin B2, vitamin B6, vitamin B12, nicotinamide, folic acid, calcium pantothenate and biotin.

[0046] The pellet feed preparation method

[0047] (1) uniformly mix the various powder feed raw materials and premixes mentioned above;

[0048] (2) prepare the feed additive according to Table 1;

[0049] Table 1 Test design

[0050] Group Type of feed additive Control group Purified water Test Example 1 Bacillus licheniformis: Saccharomyces cerevisiae (1:2) feed additive Test Example 2 Bacillus licheniformis feed additive Test Example 3 Saccharomyces cerevisiae feed additive

[0051] (3) add the feed additive prepared in step (2) to the dry feed prepared in step (1), stir uniformly, and control the moisture content of the stirred mixture to 12% to 15%, so that the mixture can form a ball when grasped by hand and immediately disperse when touched by a finger;

[0052] (4) pelletize the mixed feed prepared in step (3) using a pelletizer

[0053] (5) dry the pellet prepared in step (4) at low temperature or in the shade, do not dry in the sun, and store in a bag when the moisture content is ≤14%, and store in the shade and seal

[0054] Example 3: Rabbit feeding test using the rabbit pellet feed prepared according to Example 2

[0055] Test method: 100 male rabbits aged 25 days were selected, one rabbit per cage, and the 100 rabbits were divided into 4 groups, with a test period of 60 days, and the test process was conventional feeding, free feeding and drinking.

[0056] Data statistics: first, the test data was arranged using Excel 2019, then one-way ANOVA was performed on the test data using SPSS19.0, and the data analysis results were expressed as mean ± standard error.

[0057] Table 2 Effects of different combinations of microbial agents on diarrhea rate and production performance of rabbits (26-86d)

[0058]

[0059]

[0060] Note: The same column data with different small letters indicate significant difference (P<0.05), the same or no letter indicates no significant difference (P>0.05)3002

[0061] Table 2 shows that Bacillus licheniformis and Saccharomyces cerevisiae have certain improvement on the daily weight gain, death rate caused by diarrhea and overall death rate of rabbits. The feed additive of Bacillus licheniformis combined with Saccharomyces cerevisiae has the strongest effect on increasing the daily weight gain and reducing the death rate of rabbits.

Claims

1. A feed additive for improving the intestinal health of rabbits, characterized in that... It consists of Bacillus licheniformis and Saccharomyces cerevisiae in a mass ratio of 1:2, wherein: The viable count of the Bacillus licheniformis is ≥5×10⁻⁶. 7 CFU / mL The viable count of the brewer's yeast is ≥3×10⁻⁶. 7 CFU / mL.

2. The feed additive according to claim 1, characterized in that... The viable count of the *Bacillus licheniformis* was 6 × 10⁻⁶ 7 CFU / mL.

3. The feed additive according to claim 1, characterized in that... The viable count of the brewing yeast is 4 × 10⁻⁶. 7 CFU / mL.

4. The use of the feed additive according to any one of claims 1-3 in the preparation of pelleted feed that reduces gastrointestinal diseases in rabbits, reduces rabbit mortality, or increases rabbit growth rate.

5. The application according to claim 4, characterized in that, The pelleted feed, by weight, includes 30-35 parts alfalfa hay powder, 10-15 parts grass hay powder, 22-25 parts corn flour, 13-15 parts oilseed meal, 12-14 parts wheat bran, 1-1.5 parts light calcium carbonate, 0.7-1 part potassium dihydrogen phosphate, 1-1.5 parts trace element premix, 1-1.5 parts vitamin premix, and 0.3-0.5 parts salt.

6. The use of the feed additive according to any one of claims 1-3 in rabbit farming.

7. The application according to claim 6, characterized in that, The feed additive is added at a rate of 150-500 kg / ton to rabbit feed.

8. A method for preparing the feed additive according to any one of claims 1-3, characterized in that, Mix Bacillus licheniformis powder and Saccharomyces cerevisiae powder with pure water at 35-37℃ until homogeneous. When preparing the solution, dilute according to the number of live bacteria contained in the Bacillus licheniformis powder and Saccharomyces cerevisiae powder.

Images