Chimonobambusa auriculata transcription factor CuLQW1 as well as coding gene and application thereof

Abstract

The invention discloses a Chimonobambusa chensinensis transcription factor CuLQW1 as well as a coding gene and application thereof, belongs to the technical field of plant genetic engineering, and particularly relates to the coding gene of the Chimonobambusa chensinensis transcription factor CuLQW1, and the nucleotide sequence of the coding gene is shown as SEQ ID NO.2. The coding gene of the chimonobambusa chensinensis transcription factor CuLQW1 is applied to regulation of synthesis of lignin and cellulose of gramineous plants. The coding gene of the chimonobambusa chensinensis transcription factor CuLQW1 is applied to regulation and control of rice drought stress tolerance. The lignin and cellulose synthesis regulation transcription factor CuLQW1 and the coding gene thereof are successfully separated and identified from Chimonobambusa chensinensis, and the result shows that the transcription factor CuLQW1 participates in the regulation of lignin and cellulose synthesis, regulates the drought stress tolerance of rice, and provides a new gene resource for plant wood property improvement gene engineering.

Description

Technical Field

[0001] This invention belongs to the field of plant genetic engineering technology, and in particular relates to the transcription factor CuLQW1 of Phyllostachys edulis var. jinfoshanensis, its encoding gene, and its applications. Background Technology

[0002] Jinfo Mountain square bamboo ( Chimonobambusa utilis (Keng) PC Keng is a native bamboo species in China with both economic and ecological value. Its bamboo shoots are crisp, delicious, and nutritious, earning it the title of "King of Bamboo Shoots." A distinctive industry has developed around fresh bamboo shoots, dried bamboo shoots, and their processed products. Meanwhile, the Jinfo Mountain square bamboo has a unique culm shape and tough texture, making it widely used in construction, furniture, and bamboo handicrafts. In plant cell walls, cellulose, hemicellulose, and lignin constitute the cell wall skeleton. The content and spatial distribution of lignin and cellulose are key factors affecting the strength, toughness, and processing performance of bamboo. Studies have shown that regulating the synthesis and deposition of lignin and cellulose in cells can not only alter cell wall thickness and density, thereby improving the mechanical properties of bamboo such as bending and compressive strength, but also potentially enhance the plant's tolerance to abiotic stresses such as drought and high salinity. This is of great significance for the high-performance utilization and high-resistance breeding of bamboo.

[0003] Existing research on genes regulating cell wall metabolism in gramineous crops and some bamboo species has largely focused on the functional analysis of structural enzyme genes or a few transcription factors. Results indicate that transcription factors play a regulatory role in cell wall formation and wood property development by controlling the expression of key enzyme genes in downstream lignin and cellulose synthesis pathways. Overall, research on the fine-grained regulation of lignin and cellulose synthesis by bamboo transcription factors is still in its early stages, especially in *Phyllostachys japonicus*, where transcriptional regulation studies are lacking, and a systematic analysis of the cell wall regulatory network is even more scarce. The cloning, expression characteristics, and biological functions of transcription factors regulating lignin synthesis in *Phyllostachys japonicus* have not yet been publicly reported, and their mechanisms of action in cell wall component accumulation, wood property development, and stress resistance regulation remain a blank. Therefore, it is necessary to discover and identify key candidate genes for lignin and cellulose synthesis, including MYB-type transcription factors, from *Phyllostachys japonicus*. By using modern biotechnology methods such as gene overexpression, expression suppression, or gene editing, the properties of *Phyllostachys japonicus* and other bamboo species can be improved in a targeted manner. This will cultivate new germplasm with both excellent bamboo properties and strong stress resistance, providing a reliable molecular breeding foundation for the sustainable development of the *Phyllostachys japonicus* industry. Summary of the Invention

[0004] To address the aforementioned technical problems, this invention proposes the transcription factor CuLQW1 of Phyllostachys edulis and its encoding gene, along with its applications. Overexpression of the transcription factor CuLQW1 or its encoding gene in rice can effectively increase the lignin and cellulose content in rice, providing a new gene resource for genetic engineering for improving plant properties.

[0005] To achieve the above objectives, the present invention provides a gene encoding the transcription factor CuLQW1 of Phyllostachys edulis from Jinfo Mountain, the nucleotide sequence of which is shown in SEQ ID NO.2.

[0006] The present invention also provides the transcription factor CuLQW1 encoded by the gene, the amino acid sequence of which is shown in SEQ ID NO.1.

[0007] The present invention also provides a biological material comprising the said encoding gene.

[0008] Preferably, the biomaterial is one or more of an expression cassette, an expression vector, or engineered bacteria.

[0009] The present invention also provides the application of the coding gene, the transcription factor CuLQW1 of Phyllostachys edulis, or the biomaterial in regulating the synthesis of lignin and cellulose in grasses.

[0010] Preferably, the grass plant is rice.

[0011] Preferably, by overexpressing the encoded gene, the expression level of the transcription factor CuLQW1 of Phyllostachys edulis is increased, thereby increasing the content of lignin and cellulose in the plant.

[0012] The present invention also provides the application of the coding gene, the Jinfoshan Fangzhu transcription factor CuLQW1, or the biological material in regulating the drought stress tolerance of rice. By overexpressing the coding gene, the expression level of Jinfoshan Fangzhu transcription factor CuLQW1 is increased, thereby improving the drought stress tolerance of rice plants.

[0013] The present invention also provides a method for increasing the lignin and cellulose content in rice, comprising: overexpressing the encoding gene or the transcription factor CuLQW1 of Phyllostachys edulis in rice to increase the lignin and cellulose content in rice.

[0014] The present invention also provides a method for improving the drought stress tolerance of rice, comprising: overexpressing the encoding gene or the transcription factor CuLQW1 of Phyllostachys edulis in rice to improve the drought stress tolerance of rice plants.

[0015] Compared with the prior art, the present invention has the following advantages and technical effects: 1. This invention successfully isolated and identified the transcription factor CuLQW1, which regulates lignin and cellulose synthesis, and its encoding gene from *Phyllostachys edulis*. Functional verification was then performed by transferring CuLQW1 into wild-type rice using transgenic technology. The transgenic rice showed approximately 13.0% higher lignin content and 14.0% higher cellulose content than wild-type rice, indicating that the transcription factor CuLQW1 participates in the regulation of lignin and cellulose synthesis. The transgenic rice also exhibited significantly improved drought stress tolerance compared to wild-type rice, suggesting that the encoding gene of the transcription factor CuLQW1 regulates drought stress tolerance in rice.

[0016] 2. This invention overexpresses the transcription factor CuLQW1 or its encoding gene in rice, a monocotyledonous model plant, which can effectively increase the lignin and cellulose content in rice and enhance its drought stress tolerance. This provides a theoretical basis and technical support for the subsequent use of genetic engineering technology to improve the quality of Phyllostachys edulis. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is an agarose gel electrophoresis image of the pC1300-CuLQW1 recombinant plasmid of the present invention after double enzyme digestion. In the figure, M1 represents the DNA molecular weight marker DL15000, M2 represents the DNA molecular weight marker DL5000, and 1~3 represent the pC1300-CuLQW1 recombinant expression plasmid. Figure 2 This is a structural diagram of the pC1300-CuLQW1 overexpression vector of the present invention; Figure 3 This is a diagram of the PCR electrophoresis results of single-clone colonies in this invention. In the diagram, M represents the DNA molecular weight marker DL5000, 1 represents the empty vector negative control, and 2-4 represent the PCR results of single-clone colonies. Figure 4 The figure shows the PCR detection results of the transgenic rice plants of this invention. In the figure, M represents the DNA molecular weight marker DL5000, 1 represents the wild-type rice negative control, and 2-4 represent the transgenic rice plants. Figure 5 The figure shows the phenotypic diagrams of wild-type rice and rice lines overexpressing CuLQW1 in this invention. WT represents wild-type rice, and OE1 and OE2 represent transgenic rice lines of CuLQW1. The scale bar is 5cm. Figure 6This invention presents the phenotypic and statistical diagrams of parenchyma cell thickness in wild-type rice and CuLQW1 overexpressing rice lines. A represents the phenotypic diagram of parenchyma cell thickness in wild-type rice (scale bar: 40 μm), B represents the phenotypic diagram of parenchyma cell thickness in the CuLQW1 overexpressing rice line OE1 (scale bar: 40 μm), C represents the phenotypic diagram of parenchyma cell thickness in the CuLQW1 overexpressing rice line OE2 (scale bar: 40 μm), and D represents the statistical diagram of parenchyma cell thickness in wild-type rice and CuLQW1 overexpressing rice lines. In the diagrams, WT represents wild-type rice, and OE1 and OE2 represent CuLQW1 transgenic rice lines. "" indicates a significance level of P ≤ 0.01; Figure 7 The figure shows the lignin and cellulose content of wild-type rice and CuLQW1 transgenic lines of this invention. In the figure, A is the lignin content, B is the cellulose content, WT represents wild-type rice, and OE1 and OE2 represent the CuLQW1 transgenic rice lines. "" indicates a significance level of 0.01 < P ≤ 0.05; Figure 8 The figures show the growth phenotypes of wild-type rice and CuLQW1 transgenic lines after 2 weeks of normal and drought treatments. In the figures, A is the growth phenotype after 2 weeks of normal treatment, and B is the growth phenotype after 2 weeks of drought treatment. The scale bar is 5 cm. In the figures, WT represents wild-type rice, and OE1 and OE2 represent the CuLQW1 transgenic rice lines. Figure 9 This is a biomass analysis diagram of wild-type rice and CuLQW1 transgenic lines after 2 weeks of normal and drought stress treatments. In the diagram, A represents seedling height growth, B represents root length growth, C represents aboveground dry weight, D represents underground dry weight, E represents aboveground fresh weight, and F represents underground fresh weight. WT represents wild-type rice, OE1 and OE2 represent the CuLQW1 transgenic rice lines, and "ns" indicates P > 0.05. "" indicates a significance level of 0.01 < P ≤ 0.05. "" indicates a significance level of P ≤ 0.01; Figure 10 This is a graph showing the analysis of photosynthetic parameters and chlorophyll fluorescence parameters of wild-type rice and CuLQW1 transgenic lines after 2 weeks of normal and drought stress treatments. In the graph, A represents net photosynthetic rate, B represents stomatal conductance, C represents apparent electron transport rate, and D represents non-photochemical quenching. WT represents wild-type rice, OE1 and OE2 represent the CuLQW1 transgenic rice lines, and "ns" indicates P > 0.05. "" indicates a significance level of 0.01 < P ≤ 0.05. "" indicates a significant difference level (P ≤ 0.01). Detailed Implementation

[0019] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0020] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0021] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0022] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0023] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0024] Sources of materials used in this invention: Young bamboo shoots from Jinfo Mountain were collected from Jinfo Mountain National Scenic Area, Nanchuan District, Chongqing, at an altitude of 1850m (28°57′5″N, 107°11′28″E); pGEM-T easy vector was purchased from Promega (A1360); Escherichia coli DH5α competent cells were purchased from Beijing Zhuangmeng International Biotechnology Co., Ltd. (ZK206); PrimerSTAR Mix high-fidelity enzyme amplification enzyme was purchased from Takara (R010Q); pCAMBIA1300-Ubi-GFP-FLAG (pC1300-Ubi) vector was purchased from Miaoling plasmid platform (P29697); kanamycin resistance (50 mg·L⁻¹) was also tested. -1The following were purchased from Beijing Cooler Technology Co., Ltd. (CK6731); YEP culture medium was purchased from Beijing Cooler Technology Co., Ltd. (PM0891); Kimura B rice nutrient solution was purchased from Beijing Cooler Technology Co., Ltd. (NS1050).

[0025] Example 1 I. Obtain the encoding gene of CuLQW1, a transcription factor of Phyllostachys edulis from Jinfoshan.

[0026] Using young shoots of *Phyllostachys edulis* from Jinfo Mountain as experimental material, RNA was extracted and reverse transcribed into cDNA as a template. Specific primers were designed for the predicted gene CHU16g0313 in *Phyllostachys edulis*, and the coding region sequence was amplified by PCR.

[0027] The primer sequences are as follows: Upstream primer: 5′-AAGCTCCACAAACCTGTCAGC-3′ (SEQ ID NO.3); Downstream primer: 5′-GAACCAAAACCCAAGTATCCAA-3′ (SEQ ID NO.4).

[0028] The PCR amplification products were detected by agarose gel electrophoresis. The target band was excised, purified, and recovered. The recovered DNA fragment was ligated into the pGEM-T easy vector, transformed into E. coli DH5α competent cells, screened by blue-white screening, extracted positive clone plasmids, and analyzed by enzyme digestion pattern analysis. The single clones were then sequenced to obtain the target fragment of 1284 bp, as shown in SEQ ID NO.2.

[0029] Further online comparative analysis using BlastP software revealed that the amino acid sequence encoded by this gene (as shown in SEQ ID NO.1) is similar to that of durum wheat (…). Triticum turgidum subsp.durum The homologous protein (VAH02014.1) showed the highest identity (92.09%) with barley (…). Hordeum vulgare The homology of the MYB family homolog (KAE8806364.1) was 92.07%. Protein domain analysis showed that the protein possesses conserved domains related to the MYB family, with two structures, R2 and R3, at the N-terminus, with structural models of [-W-(X19)-W-(X19)-W-] and [-F-(X18)-W-(X18)-W-]. Therefore, the cloned coding gene belongs to R2R3-MYB. This gene was named the coding gene of the *Phyllostachys edulis* transcription factor CuLQW1, and the pGEM-T easy vector containing this gene was named pT-CuLQW1.

[0030] The amino acid sequence of the transcription factor CuLQW1 of Chimonobambusa utilis McClure var. utilis Hsueh et Keng f. from Jinfo Mountain is shown in SEQ ID NO.1, SEQ ID NO.1: MGRHSCCYKQKLRKGLWSPEEDEKLMNHITKHGHGCWSSVPKLAGLQRCGKSCRLRWINYLRPDLKRGAFSQEEEDLIIELHTVLGNRWSQIAAQLPGRTDNEIKNLWNSSLKKKLRQRGIDPNTHKPLAEVDRSGAAPTISTERTSGSSDVNPSSAGTLGNLSHLLSETAQSSMLLPVYDKNRPETPSLARPKGPPKELFLDQLPAGHESPSSCRSSGPTLYFPFQQPLGYSNECGSGNGANMNSLWFNQNDFNCSTISTVMPPVSPSALSTSMGLNLPPDNPRHGGTGIGSAPFYWGGANPSSSSSTGSNGSNGMGFEPQCTNSILENSVFPWTDIGQEKDTRVHLVEELKWPDLLHGTFAEATAAMQNQSQSLYDDVIKAESQFNMEGICASWFQNQQPQQQLQAPSDMYDKDLQRMPLSFEHI。

[0031]

[0032] II. Constructing a plant expression vector carrying the coding gene of the transcription factor CuLQW1 from Phyllostachys edulis.

[0033] Based on the coding gene of the transcription factor CuLQW1 from Phyllostachys edulis shown in SEQ ID NO:2, amplification primers with homologous arms and restriction enzyme sites BamHⅠ / PmlⅠ were designed. The obtained correctly sequenced plasmid was diluted 50 times and used as a template. Using PrimerSTAR Mix high-fidelity amplification enzyme, PCR amplification was performed on the fragment containing the restriction enzyme sites (BamHⅠ / PmlⅠ) required for expression vector construction and the coding gene of the transcription factor CuLQW1 from Phyllostachys edulis.

[0034] The primer sequences are as follows: Upstream primer: 5′-GTTGTTTGGTGTTACTTCTGCAGggatccATGGGGAGGCATTCTTGTTGC-3′ (SEQ ID NO.5, lowercase letter of BamHI site); Downstream primer: 5′-GCCCTTGCTCACCATAGGCCTcacgtgGATATGCTCAAAAGACAAGGGCATTCT-3′ (SEQ ID NO.6, lowercase letter of PmlⅠ site); The PCR amplification products were detected by agarose gel electrophoresis, the target band was excised and purified, and the target gene and the enzyme digestion fragment of the pCAMBIA1300-Ubi-GFP-FLAG (pC1300-Ubi) vector were ligated using one-step cloning technology. The reaction system is shown in Table 1 below (10 μL).

[0035] Table 1 Reaction System

[0036] Add all reagents from Table 1 to the PCR tube in the order listed in Table 1, mix well, centrifuge, place in a PCR instrument, and incubate at 50°C for 5 min to transform *E. coli* DH5α. Extract plasmids and identify them using restriction enzyme patterns (e.g., ...). Figure 1 After sequencing verification, the obtained recombinant expression vector was named pC1300-CuLQW1 (e.g., ...). Figure 2 ).

[0037] III. Identification of monoclonal colonies containing the expression vector pC1300-CuLQW1.

[0038] Due to rice ( Oryza sativa Both *Agrobacterium* and bamboo are monocotyledonous grasses and are very closely related. Therefore, the recombinant expression vector pC1300-CuLQW1 was transformed into *Agrobacterium* using the freeze-thaw method. Agrobacterium tumefacien. Strain GV3101 competent cells were plated in kanamycin-resistant (50 mg / L) culture medium. -1 After incubation at 28°C on plates, single colonies were picked and cultured in YEP medium for bacterial PCR identification. Using single colonies transformed with the recombinant expression vector as templates, PCR detection was performed using primers with nucleotide sequences shown in SEQ ID NO. 5 and SEQ ID NO. 6, with an empty vector serving as a negative control.

[0039] like Figure 3 As shown in the figure, the PCR electrophoresis results of Agrobacterium monoclonal colonies transformed by the expression vector pC1300-CuLQW1 plasmid indicate that the monoclonal colonies contain the target gene fragment. After shaking, Agrobacterium monoclonal bacterial suspensions were obtained and can be used for infection and transformation experiments.

[0040] IV. Transformation of the coding gene of CuLQW1, a transcription factor from Phyllostachys edulis, into rice and PCR verification.

[0041] Using the obtained Agrobacterium monoclonal bacterial suspension, rice callus was infected using the Agrobacterium-mediated method, and resistance (hygromycin 50 mg·L⁻¹) was obtained. -1 After screening, differentiation and rooting steps, two transgenic rice lines, OE1 and OE2, were finally obtained and their gene expression was detected.

[0042] Total RNA was extracted from CuLQW1 transgenic rice plants (OE1 and OE2) and wild-type (WT) plants and reverse transcribed into cDNA. Using this cDNA as a template, PCR detection was performed using primers with nucleotide sequences shown in SEQ ID NO.5 and SEQ ID NO.6.

[0043] like Figure 4 As shown, the results indicated that the target gene was detected in the CuLQW1 transgenic rice plants (OE1 and OE2), proving that CuLQW1 had been transferred into the rice plants, while no expression was detected in wild-type rice plants (WT). Wild-type rice (ET) and the validated transgenic plants (OE1 and OE2) were planted in the field, and T1 generation seeds were harvested. After germination, they were hydroponically cultured in a light incubator for two weeks. The conditions of the light incubator were set as follows: temperature 28±2℃, 12h light, 12h dark, and relative humidity 60%. The nutrient solution used for hydroponics was Kimura B rice nutrient solution. Rice seedlings suitable for subsequent experiments were obtained (e.g., Figure 5 ).

[0044] V. Analysis of cell wall thickness in transgenic rice thin-walled cells.

[0045] The obtained CuLQW1 transgenic rice seedlings (OE1 and OE2) were cultured to maturity, and the stem bases were harvested for scanning electron microscopy to observe and count the thickness of parenchyma cells. Analysis revealed that, for example... Figure 6 China A Figure 6 B, Figure 6 C and Figure 6 As shown in Figure D, the parenchyma cell thickness of the rice CuLQW1 transgenic lines OE1 and OE2 was significantly higher than that of WT, indicating that the transgenic CuLQW1 can increase the cell wall thickness of rice stem parenchyma cells.

[0046] VI. Analysis of lignin and cellulose content in transgenic rice.

[0047] The lignin and cellulose contents were determined by chemical methods from the stems of mature wild-type (WT) and transgenic rice lines (OE1 and OE2).

[0048] like Figure 7 China A and Figure 7 As shown in Figure B, the results indicated that, compared to the wild-type (WT), the lignin content in the CuLQW1 transgenic rice lines OE1 and OE2 increased by 13.85% and 15.08%, respectively, and the cellulose content increased by 12.96% and 13.90%, respectively. In conclusion, overexpression of the gene encoding the transcription factor CuLQW1 from *Phyllostachys jinfoshanensis* can increase the lignin and cellulose content in rice.

[0049] VII. Comparative analysis of biomass of transgenic rice under drought stress.

[0050] Wild-type rice seedlings (WT) and transgenic rice CuLQW1 seedlings (OE1 and OE2) were subjected to drought treatment (10% PEG6000 by mass) for 2 weeks. Seedlings before and after treatment (e.g., Figure 8 China A and Figure 8 Statistical analysis was performed on the biomass of B), including the increase in seedling height and root length, and the fresh and dry weight of the aboveground and underground plants.

[0051] Statistical analysis found that, for example Figure 9 China A Figure 9 B, Figure 9 C, Figure 9 D, Figure 9 China E and Figure 9As shown in Figure F, before drought treatment, there was no significant difference in aboveground and belowground biomass between CuLQW1 transgenic rice seedlings OE1 and OE2 and wild-type rice seedlings (WT). After drought treatment, compared with wild-type rice seedlings (WT), the overexpressing CuLQW1 transgenic lines OE1 and OE2 showed significantly increased seedling height and root length, as well as significantly increased whole-plant dry and fresh biomass. The seedling height increase of CuLQW1 overexpressing transgenic lines OE1 and OE2 was 1.37 times and 1.37 times the root weight (WT) of wild-type rice seedlings, respectively; the root length increase was 1.29 times and 1.25 times the WT, respectively. The aboveground fresh weight and dry weight of CuLQW1 overexpressing transgenic lines OE1 and OE2 were 1.44 times and 1.39 times the WT, respectively, of wild-type rice seedlings, respectively. The underground fresh weight and dry weight of CuLQW1 overexpressing transgenic lines OE1 and OE2 were 1.69 times and 1.70 times the WT, respectively, of wild-type rice seedlings, respectively. These results indicate that CuLQW1 overexpression significantly improves the drought stress tolerance of transgenic rice plants.

[0052] VIII. Comparative analysis of photosynthetic and fluorescence parameters of transgenic rice under drought stress.

[0053] The obtained rice seedlings were subjected to drought treatment. The photosynthetic parameters of the rice seedlings before and after treatment were detected using a portable photosynthesis meter, and the chlorophyll fluorescence parameters were measured using a dual-channel chlorophyll fluorescence meter.

[0054] Statistical analysis found that, for example Figure 10 China A Figure 10 B, Figure 10 C and Figure 10 As shown in Figure D, before drought treatment, there were no significant differences in net photosynthetic rate, stomatal conductance, apparent electron transport rate (ETR), and non-photochemical quenching (NPQ) between the CuLQW1 overexpressing transgenic lines OE1 and OE2. Drought stress significantly reduced the photosynthetic capacity of wild-type rice plants (WT). After drought treatment, the net photosynthetic rate in wild-type rice plants (WT) was 6.6030 µmol / m². 2 / s, porosity is 0.2052 mol / m 2 / sl, ETR is 23.7583μmol electrons / m 2 / s; The net photosynthetic rates of the CuLQW1 transgenic lines OE1 and OE2 were 150.61% (9.9445 µmol / m²) of those of wild-type rice plants (WT). 2 / s) and 157.62% (10.4077µmol / m 2 / s), and stomatal conductance was 188.57% (0.3869 mol / m) of that of wild-type rice plants (WT). 2 / s) and 191.71% (0.3935mol / m 2 / s), ETR is 151.89% (36.0877 μmol electrons / m) of wild-type rice plants (WT). 2 / s) and 152.73% (36.2850 μmol electrons / m 2 / s). This indicates that overexpression of CuLQW1 improves the tolerance of transgenic rice to drought stress.

[0055] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A gene encoding the transcription factor CuLQW1 from *Phyllostachys edulis*, characterized in that, The nucleotide sequence of the encoding gene is shown in SEQ ID NO.

2.

2. The transcription factor CuLQW1 encoded by the gene of *Phyllostachys edulis* as described in claim 1, characterized in that, The amino acid sequence of the transcription factor CuLQW1 is shown in SEQ ID NO.

1.

3. A biological material comprising the gene encoding as described in claim 1.

4. The biomaterial according to claim 3, characterized in that, The biological material is one or more of the following: expression cassette, expression vector, or engineered bacteria.

5. The application of the encoding gene as described in claim 1, the transcription factor CuLQW1 of Phyllostachys edulis as described in claim 2, or the biomaterial as described in claim 3 or 4 in regulating lignin and cellulose synthesis in grasses.

6. The application according to claim 5, characterized in that, The grass species mentioned is rice.

7. The application according to claim 5, characterized in that, Overexpression of the encoded gene increased the expression level of the transcription factor CuLQW1 in Phyllostachys edulis, thereby increasing the content of lignin and cellulose in the plant.

8. The application of the encoding gene as described in claim 1, the *Phyllostachys edulis* transcription factor CuLQW1 as described in claim 2, or the biomaterial as described in claim 3 or 4 in regulating drought stress tolerance in rice, characterized in that... Overexpression of the encoded gene increased the expression level of the transcription factor CuLQW1 in Phyllostachys edulis, thereby improving the drought stress tolerance of rice plants.

9. A method for increasing the lignin and cellulose content in rice, characterized in that, include: Overexpression of the encoding gene described in claim 1 or the transcription factor CuLQW1 of Phyllostachys edulis described in claim 2 in rice increases the lignin and cellulose content in rice.

10. A method for improving the drought stress tolerance of rice, characterized in that, include: Overexpression of the encoding gene described in claim 1 or the transcription factor CuLQW1 of Phyllostachys edulis described in claim 2 in rice can improve the drought stress tolerance of rice plants.

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