Composition for reducing blood fat and blood sugar and improving blood pressure and application thereof

Abstract

The invention relates to a composition for reducing blood fat and blood sugar and improving blood pressure and application thereof, and belongs to the technical field of traditional Chinese medicines. The technical problems that in the prior art, the technology of thirst quenching decoction and gastrodia elata and uncaria decoction prescriptions is backward, and the dosage is large are solved. The composition consists of the following traditional Chinese medicine extracts in parts by mass: 3-9 parts of a traditional Chinese medicine extract A, 2-4 parts of a traditional Chinese medicine extract B, 5-7 parts of a traditional Chinese medicine extract C and 2-6 parts of a traditional Chinese medicine extract D, according to the invention, classic prescriptions of diabetes decoction and gastrodia elata and uncaria drink are applied, effective components are extracted and separated through modern science and technology, effective extracts are obtained, and the curative effect is exerted through a corresponding proportion. Pharmacodynamic research shows that the traditional Chinese medicine composition can achieve the effects of reducing blood fat and blood sugar and improving blood pressure through the effects of clearing stomach heat and nourishing kidney yin. And the related process is advanced, and the dose is not large.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a composition for lowering blood lipids, blood sugar and improving blood pressure and its application. Background Technology

[0002] The ancient classic formula, Tianma Gouteng Decoction, is clinically used to treat hypertension, acute cerebrovascular disease, and inner ear vertigo, which are attributed to liver yang hyperactivity and liver wind disturbance. The formula consists of: Gastrodia elata, Uncaria rhynchophylla, Haliotis diversicolor, Gardenia jasminoides, Scutellaria baicalensis, Achyranthes bidentata, Eucommia ulmoides, Leonurus japonicus, Taxillus chinensis, Polygonum multiflorum, and Poria cocos. Its effects are: calming the liver and extinguishing wind, clearing heat and promoting blood circulation, and tonifying the liver and kidneys.

[0003] The ancient classic formula Xiaoke Decoction is mainly used for diabetes mellitus caused by deficiency of both Qi and Yin. The main ingredients of Xiaoke Decoction are clam meat, Rehmannia glutinosa, mulberry fruit, and Asparagus cochinchinensis. Its effects are: nourishing the five internal organs, quenching thirst, and resolving phlegm and fluid retention.

[0004] However, the main drawbacks of the aforementioned ancient classic prescriptions, Xiaoke Decoction and Tianma Gouteng Decoction, are that their processing techniques are relatively outdated and the dosage is large. Summary of the Invention

[0005] This invention addresses the technical problems of outdated processes and large dosages in existing Xiaoke Decoction and Tianma Gouteng Decoction formulas, providing a composition for lowering blood lipids, blood sugar, and improving blood pressure, and its application. The composition of this invention utilizes classic formulas like Xiaoke Decoction and Tianma Gouteng Decoction, employing modern scientific techniques to extract and separate the effective components, obtaining an effective extract. Through appropriate formulation, it exerts its therapeutic effect. Pharmacological studies show that the traditional Chinese medicine composition of this invention can lower blood lipids, blood sugar, and improve blood pressure by clearing stomach heat and nourishing kidney yin. The traditional Chinese medicine composition of this invention has a significant antihypertensive effect, exerting its effect of lowering the "three highs" (high blood pressure, high blood sugar, and high cholesterol) by regulating lipid metabolism and blood sugar levels. Furthermore, the involved process is advanced, and the dosage is small.

[0006] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows: A composition for lowering blood lipids, blood sugar, and improving blood pressure, comprising the following parts by weight of traditional Chinese medicine extracts: Chinese herbal extract A: 3-9 parts; Chinese herbal extract B: 2-4 parts; Chinese herbal extract C: 5-7 parts; Chinese herbal extract D: 2-6 parts; The preparation method of traditional Chinese medicine extract A includes the following steps: Gastrodia elata, Rehmannia glutinosa, Cassia tora, and Gardenia jasminoides were mixed, pectinase and water were added, and microwave extraction was performed. The extract was collected and freeze-dried to obtain Chinese herbal extract A. The preparation method of traditional Chinese medicine extract B includes the following steps: Gastrodia elata was extracted using a continuous reflux extraction method. The extract was collected, filtered, concentrated, and freeze-dried to obtain traditional Chinese medicine extract B. The preparation method of traditional Chinese medicine extract C includes the following steps: After mixing Rehmannia glutinosa, Gardenia jasminoides and Cornus officinalis, water ultrasonic extraction was performed. The extract was collected, filtered, concentrated, polyethylene glycol and polydextrose were added, the mixture was vortexed and centrifuged, the supernatant was collected and freeze-dried to obtain Chinese herbal extract C. The preparation method of traditional Chinese medicine extract D includes the following steps: After mixing cassia seeds and gastrodia elata, the mixture was extracted with water multiple times. The extracts were collected, filtered, concentrated, and freeze-dried to obtain herbal extract D.

[0007] Preferably, the composition consists of the following parts by weight of traditional Chinese medicine extract: 6 portions of herbal extract A, 2 portions of herbal extract B, 6 portions of herbal extract C, and 6 portions of herbal extract D.

[0008] Preferably, in the preparation method of traditional Chinese medicine extract A, the mass ratio of Gastrodia elata, Rehmannia glutinosa, Cassia tora, and Gardenia jasminoides is 4:6:8:4.

[0009] Preferably, in the preparation method of traditional Chinese medicine extract A, the microwave extraction power is 320w and the extraction time is 60min.

[0010] Preferably, in the preparation method of traditional Chinese medicine extract B, the mass fraction of Gastrodia elata is 15 parts.

[0011] Preferably, in the preparation method of traditional Chinese medicine extract B, the number of times of continuous reflux extraction is 2, and the extraction time is 1.5h each time.

[0012] Preferably, in the preparation method of traditional Chinese medicine extract C, the mass ratio of Rehmannia glutinosa, Gardenia jasminoides, and Cornus officinalis is 20:10:10, and the ultrasonic extraction time is 30 min.

[0013] Preferably, in the preparation method of the traditional Chinese medicine extract D, the mass ratio of cassia seed and gastrodia is 10:7, the water extraction is performed twice, and the extraction time is 1.5h each time.

[0014] The use of a composition of the present invention in the preparation of a medicament for treating high blood lipids, high blood sugar, and high blood pressure.

[0015] The beneficial effects of this invention are: The composition of this invention for lowering blood lipids, blood sugar, and improving blood pressure includes 3-9 parts of traditional Chinese medicine extract A, 2-4 parts of traditional Chinese medicine extract B, 5-7 parts of traditional Chinese medicine extract C, and 2-6 parts of traditional Chinese medicine extract D. Each extract is obtained by extraction, separation, and purification of the effective components of different herbs using scientific and technological methods and instruments. Traditional Chinese medicine extract A is the polysaccharide portion of Gastrodia elata, Rehmannia glutinosa, Cassia tora, and Gardenia jasminoides; traditional Chinese medicine extract B is the polyphenol portion of Gastrodia elata; traditional Chinese medicine extract C is the iridoid ether terpenoid portion of Rehmannia glutinosa, Gardenia jasminoides, and Cornus officinalis; and traditional Chinese medicine extract D is the organic acid portion of Cassia tora and Gastrodia elata. Through reasonable pharmacodynamic experiments, the different extracts are combined in optimal proportions to enhance the absorption and efficacy of the active ingredients. Pharmacodynamic experiments have verified that the obtained traditional Chinese medicine composition has a significant antihypertensive effect. Blood sugar and blood lipid level tests show that the composition of this invention lowers blood pressure, blood sugar, and cholesterol by regulating lipid metabolism and blood sugar levels. The effect of the fourth example group is the best, and it is superior to the positive control group and other composition groups under the same dosage conditions. Furthermore, the process involved is advanced, and the dosage is small. Detailed Implementation

[0016] The inventive concept of this invention is as follows: Based on the ancient classic prescriptions Xiaoke Decoction and Tianma Gouteng Decoction, this invention uses Gastrodia elata, Cassia tora, Gardenia jasminoides, Rehmannia glutinosa, and Cornus officinalis as the main medicinal materials. Different methods are used to extract, separate, enrich, and purify each component to obtain extracts of different components. The extracts are then rationally proportioned, formulated, and processed into a preparation. Through animal experiments, a composition that lowers blood lipids and blood sugar and improves blood pressure and its application are obtained.

[0017] This invention utilizes classic formulas such as Xiaoke Decoction and Tianma Gouteng Decoction, and extracts and separates the effective components using modern scientific techniques to obtain effective extracts. Through appropriate formulation, these extracts exert their therapeutic effects. Pharmacological studies have shown that the traditional Chinese medicine composition of this invention can lower blood lipids and blood sugar and improve blood pressure by clearing stomach heat and nourishing kidney yin.

[0018] This invention provides a composition for lowering blood lipids, blood sugar, and improving blood pressure, comprising the following parts by weight of traditional Chinese medicine extracts: Chinese herbal extract A: 3-9 parts; Chinese herbal extract B: 2-4 parts; Chinese herbal extract C: 5-7 parts; Chinese herbal extract D: 2-6 parts; The preparation method of traditional Chinese medicine extract A includes the following steps: Gastrodia elata, Rehmannia glutinosa, Cassia tora, and Gardenia jasminoides were mixed, pectinase and water were added, and microwave extraction was performed. The extract was collected and freeze-dried to obtain Chinese herbal extract A. The preparation method of traditional Chinese medicine extract B includes the following steps: Gastrodia elata was extracted using a continuous reflux extraction method. The extract was collected, filtered, concentrated, and freeze-dried to obtain traditional Chinese medicine extract B. The preparation method of traditional Chinese medicine extract C includes the following steps: After mixing Rehmannia glutinosa, Gardenia jasminoides and Cornus officinalis, water ultrasonic extraction was performed. The extract was collected, filtered, concentrated, polyethylene glycol and polydextrose were added, the mixture was vortexed and centrifuged, the supernatant was collected and freeze-dried to obtain Chinese herbal extract C. The preparation method of traditional Chinese medicine extract D includes the following steps: After mixing cassia seeds and gastrodia elata, the mixture was extracted with water multiple times. The extracts were collected, filtered, concentrated, and freeze-dried to obtain herbal extract D.

[0019] In this invention, the mass fraction of the herbal extract A can be any value between 3 and 9 parts, such as 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 9 parts, etc., preferably 3 parts, 6 parts, 9 parts, and most preferably 6 parts.

[0020] In this invention, the mass fraction of the herbal extract B can be any value between 2 and 4 parts, such as 2 parts, 3 parts, 4 parts, etc., with 2 parts being the most preferred.

[0021] In this invention, the mass fraction of the herbal extract C can be any value between 5 and 7 parts, such as 5 parts, 6 parts, 7 parts, etc., with 6 parts being the most preferred.

[0022] In this invention, the mass fraction of the herbal extract D can be any value between 2 and 6 parts, such as 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, etc., preferably 2 parts, 4 parts, 6 parts, and most preferably 6 parts.

[0023] In this invention, the composition is most preferably composed of the following parts by weight of traditional Chinese medicine extracts: 6 parts of traditional Chinese medicine extract A, 2 parts of traditional Chinese medicine extract B, 6 parts of traditional Chinese medicine extract C, and 6 parts of traditional Chinese medicine extract D.

[0024] In this invention, there are no special requirements for the preparation process and raw material dosage of the herbal extract A. Extraction can be performed using conventional methods in the field. The raw material dosages and process parameters given in this invention are preferred dosages and process parameters. For example, for freeze-drying temperature and time not given in this invention, conventional settings in the field can be used. In the preparation method of the herbal extract A of this invention, the preferred mass ratio of Gastrodia elata, Rehmannia glutinosa, Cassia tora, and Gardenia jasminoides is 4:6:8:4. After weighing the medicinal materials according to the ratio, pectinase is added at 3% of the total mass of the medicinal materials. The pectinase is preferably SPE-010 type pectinase. Water is added at a mass of 10 times the mass of the medicinal materials. Then, the mixture is placed in a microwave extractor, and microwave extraction is performed at 320W for 60 minutes. The extract is collected, freeze-dried, and the herbal extract A is obtained. The herbal extract A obtained by this method is the polysaccharide portion of Gastrodia elata, Rehmannia glutinosa, Cassia tora, and Gardenia jasminoides.

[0025] In this invention, there are no special requirements for the preparation method and raw material dosage of the traditional Chinese medicine extract B. Extraction can be performed using conventional methods in the field. The raw material dosages and process parameters given in this invention are preferred dosages and process parameters. For example, for freeze-drying temperature and time not given in this invention, conventional settings in the field can be used. In the preferred method for preparing traditional Chinese medicine extract B of this invention, the following steps are performed: 15 parts by weight of Gastrodia elata are pulverized into a fine powder and passed through a 60-mesh sieve. The mixture is then continuously refluxed twice. The first extraction uses 10 times the amount of water as the medicinal material, and the second extraction uses 8 times the amount of water as the medicinal material, each extraction lasting 1.5 hours. The extract is then collected, filtered through a 400-mesh filter cloth, concentrated to a thick paste with a relative density of 1.10~1.15 at 70°C, and freeze-dried to obtain traditional Chinese medicine extract B. The traditional Chinese medicine extract B obtained by this method is the polyphenolic fraction of Gastrodia elata.

[0026] In this invention, there are no special requirements for the preparation process and raw material dosage of the traditional Chinese medicine extract C. Extraction can be performed using conventional methods in the field. The raw material dosages and process parameters given in this invention are preferred dosages and process parameters. For example, for freeze-drying temperature and time not given in this invention, conventional settings in the field can be used. In the preparation method of the traditional Chinese medicine extract C of this invention, the preferred method is as follows: the mass ratio of Rehmannia glutinosa, Gardenia jasminoides, and Cornus officinalis is 20:10:10. The extract is pulverized into a fine powder, passed through a 60-mesh sieve, and then extracted with 4 times the amount of water by ultrasonic extraction for 30 minutes. The extract is filtered through a 400-mesh filter cloth and concentrated to a thick paste with a relative density of 1.10~1.15 at 70°C. 15% of polyethylene glycol 6000 and 10% of polydextrose 40000 of the paste volume are added, and the mixture is vortexed for 60 minutes. The mixture is then centrifuged at 10000 rad / min for 15 minutes at 25°C. The supernatant is collected and freeze-dried to obtain the traditional Chinese medicine extract C. The herbal extract C extracted by this method consists of iridoid fractions from Rehmannia glutinosa, Gardenia jasminoides, and Cornus officinalis.

[0027] In this invention, there are no special requirements for the preparation process and raw material dosage of the herbal extract D. Extraction can be performed using conventional methods in the field. The raw material dosages and process parameters given in this invention are preferred dosages and process parameters. For example, for freeze-drying temperature and time not given in this invention, conventional settings in the field can be used. In the preferred method for preparing herbal extract D of this invention, the mass ratio of cassia seed and gastrodia elata is 10:7. The extracts are pulverized into fine powder, passed through a 60-mesh sieve, and extracted twice with 10 times the amount of water, each time for 1.5 hours. The decoctions are combined, filtered through a 400-mesh sieve, concentrated to a thick paste with a relative density of 1.15~1.20 at 70℃, and freeze-dried to obtain herbal extract D. The herbal extract D extracted by this method is the organic acid fraction of cassia seed and gastrodia elata.

[0028] Finally, the Chinese herbal extracts A, B, C, and D are mixed in any proportion within the above-mentioned mass ratio range to obtain the composition of the present invention that lowers blood lipids, blood sugar, and improves blood pressure.

[0029] The present invention also includes the use of the above-mentioned composition in the preparation of a drug for treating high blood lipids, high blood sugar, and high blood pressure.

[0030] The technical solution of the present invention will be described more clearly and in detail below with reference to the embodiments. The scope of protection of the present invention is not limited by the following embodiments.

[0031] The herbal extracts used in the following examples were prepared as follows: The preparation method of traditional Chinese medicine extract A includes the following steps: Take 4 parts of Gastrodia elata, 6 parts of Rehmannia glutinosa, 8 parts of Cassia tora, and 4 parts of Gardenia jasminoides. Grind them into a fine powder, pass through an 80-mesh sieve, add 3% of the amount of SPE-010 pectinase (by weight of the herbs), mix well, pour into a beaker, add 10 times the amount of water, place in a microwave extractor, control the microwave power at 320w, microwave extract for 60 minutes, collect the extract, freeze dry, and obtain a traditional Chinese medicine extract A that lowers blood lipids, blood sugar, and improves blood pressure.

[0032] The preparation method of traditional Chinese medicine extract B includes the following steps: Take 15 parts of Gastrodia elata, grind it into fine powder, pass it through a 60-mesh sieve, and extract it by reflux twice. Add 10 times the amount of water to the first extraction and 8 times the amount of water to the second extraction. Each extraction lasts for 1.5 hours. Collect the extract, filter it through a 400-mesh filter cloth, concentrate it to a thick paste with a relative density of 1.15 at 70℃, freeze-dry it, and obtain Chinese herbal extract B that can lower blood lipids, blood sugar and improve blood pressure.

[0033] The preparation method of traditional Chinese medicine extract C includes the following steps: Take 20 parts of Rehmannia glutinosa, 10 parts of Gardenia jasminoides, and 10 parts of Cornus officinalis, grind them into fine powder, pass them through a 60-mesh sieve, add 4 times the amount of water of the medicinal materials, and extract by ultrasonic extraction for 30 minutes. Filter with a 400-mesh filter cloth and concentrate to a thick paste with a relative density of 1.15 at 70℃. Add 15% of polyethylene glycol 6000 and 10% of polydextrose 40000 to the paste, vortex for 60 minutes, and then centrifuge at 10000 rad / min for 15 minutes in a low-temperature centrifuge at 25℃. Take the supernatant, freeze dry it, and obtain the Chinese herbal extract C that lowers blood lipids, blood sugar and improves blood pressure.

[0034] The preparation method of traditional Chinese medicine extract D includes the following steps: Take 10 parts of cassia seed and 7 parts of gastrodia elata, grind them into fine powder, pass them through a 60-mesh sieve, add 10 times the amount of water of the medicinal materials and extract twice, each time for 1.5 hours, combine the decoctions, filter through a 400-mesh sieve, concentrate to a thick paste with a relative density of 1.15 at 70℃, freeze dry, and obtain the Chinese herbal extract D that lowers blood lipids, blood sugar and improves blood pressure.

[0035] The freeze-drying temperature and time during the preparation of the above four extracts can be set according to the conventional settings in the prior art, and there are no special requirements for this invention.

[0036] The screening experiments of the extracts contained in the composition of the present invention for lowering blood lipids, blood sugar and improving blood pressure are as follows: The composition of this invention comprises traditional Chinese medicine extracts A, B, C, and D. Using the mass fractions of extracts A, B, C, and D as factors, three levels are determined for each factor, and an orthogonal experimental table is designed as follows. The extracts are mixed according to the above proportions to prepare nine groups of extracts: I, II, III, IV, V, VI, VII, VIII, and IX. After thorough mixing, the final product is obtained. The nine mixtures are then subjected to efficacy evaluation studies to comprehensively evaluate their effects in lowering blood lipids, blood sugar, and improving blood pressure, and the optimal group of extracts is selected.

[0037]

[0038] Preparation of the composition of the present invention: According to the table below, the extracts are mixed evenly to prepare nine groups of compositions: I, II, III, IV, V, VI, VII, VIII, and IX.

[0039]

[0040] The following are examples of the preparation of the composition for lowering blood lipids, blood sugar and improving blood pressure according to the present invention.

[0041] Example 1: According to the mass ratio: take 3 parts of the prepared Chinese herbal extract A, 2 parts of Chinese herbal extract B, 5 parts of Chinese herbal extract C, and 2 parts of Chinese herbal extract D, and mix them thoroughly to obtain the composition I of the present invention for lowering blood lipids, blood sugar, and improving blood pressure.

[0042] Example 2: According to the mass ratio: take 3 parts of the prepared Chinese herbal extract A, 3 parts of Chinese herbal extract B, 6 parts of Chinese herbal extract C, and 4 parts of Chinese herbal extract D, and mix them thoroughly to obtain the composition II of the present invention for lowering blood lipids, blood sugar and improving blood pressure.

[0043] Example 3: According to the mass ratio: take 3 parts of the prepared Chinese herbal extract A, 4 parts of Chinese herbal extract B, 7 parts of Chinese herbal extract C, and 6 parts of Chinese herbal extract D, and mix them thoroughly to obtain the composition III of the present invention for lowering blood lipids, blood sugar, and improving blood pressure.

[0044] Example 4: According to the mass ratio: take 6 parts of the prepared Chinese herbal extract A, 2 parts of Chinese herbal extract B, 6 parts of Chinese herbal extract C, and 6 parts of Chinese herbal extract D, and mix them thoroughly to obtain the composition IV of the present invention for lowering blood lipids, blood sugar and improving blood pressure.

[0045] Example 5: By mass ratio: take 6 parts of the prepared Chinese herbal extract A, 3 parts of Chinese herbal extract B, 7 parts of Chinese herbal extract C, and 2 parts of Chinese herbal extract D, and mix them thoroughly to obtain the composition V of the present invention for lowering blood lipids, blood sugar, and improving blood pressure.

[0046] Example 6: By mass ratio: take 6 parts of the prepared Chinese herbal extract A, 4 parts of Chinese herbal extract B, 5 parts of Chinese herbal extract C, and 4 parts of Chinese herbal extract D, and mix them thoroughly to obtain the composition VI of the present invention for lowering blood lipids, blood sugar, and improving blood pressure.

[0047] Example 7: According to the mass ratio: take 9 parts of the prepared Chinese herbal extract A, 2 parts of Chinese herbal extract B, 7 parts of Chinese herbal extract C, and 4 parts of Chinese herbal extract D, and mix them thoroughly to obtain the composition VII of the present invention for lowering blood lipids, blood sugar, and improving blood pressure.

[0048] Example 8: According to the mass ratio: take 9 parts of the prepared Chinese herbal extract A, 3 parts of Chinese herbal extract B, 5 parts of Chinese herbal extract C, and 6 parts of Chinese herbal extract D, and mix them thoroughly to obtain the composition VIII of the present invention for lowering blood lipids, blood sugar, and improving blood pressure.

[0049] Example 9: According to the mass ratio: take 9 parts of the prepared Chinese herbal extract A, 4 parts of Chinese herbal extract B, 6 parts of Chinese herbal extract C, and 2 parts of Chinese herbal extract D, and mix them thoroughly to obtain the composition IX of the present invention for lowering blood lipids, blood sugar, and improving blood pressure.

[0050] Comparative Example 1: By mass ratio: take 2 parts of the prepared Chinese herbal extract A, 6 parts of Chinese herbal extract B, 6 parts of Chinese herbal extract C, and 4 parts of Chinese herbal extract D, and mix them thoroughly to obtain a composition that lowers blood lipids, blood sugar, and improves blood pressure.

[0051] Comparative Example 2: By mass ratio: take 6 parts of the prepared Chinese herbal extract A, 9 parts of Chinese herbal extract B, 4 parts of Chinese herbal extract C, and 7 parts of Chinese herbal extract D, and mix them thoroughly to obtain a composition that lowers blood lipids, blood sugar, and improves blood pressure.

[0052] Comparative Example 3: By mass ratio: take 5 parts of the prepared Chinese herbal extract A, 8 parts of Chinese herbal extract B, 3 parts of Chinese herbal extract C, and 7 parts of Chinese herbal extract D, and mix them thoroughly to obtain a composition that lowers blood lipids, blood sugar, and improves blood pressure.

[0053] The following are comparative pharmacodynamic experiments of twelve groups of compositions prepared in Examples 1-9 and Comparative Examples 1-3 of the present invention.

[0054] I. Establishment of a Hypertensive Mouse Model After one week of acclimatization feeding, C57 / J mice were randomly divided into 15 groups of 10 mice each: a blank control group, a model group, a positive control group (i.e., Zhenju antihypertensive tablets group, 0.11 g / kg, prepared with physiological saline to a drug concentration of 0.011 g / mL), Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, Example 7 group, Example 8 group, Example 9 group, and comparative example 1, comparative example 2, and comparative example 3 groups. The blank control group was fed a blank diet, while the other groups were fed a high-fat diet. After 6 weeks, when the body weight of the model group mice exceeded that of the control group mice by 20%, mice with a systolic blood pressure >120 mmHg were selected, indicating successful establishment of the hypertension model.

[0055] Intervention: On the second day after successful modeling, the drug administration groups were administered 0.11 g / kg of the corresponding drug by gavage. Specifically, the groups of Examples 1-9 were administered 0.11 g / kg of the compositions I-IX prepared in the above examples by gavage, the groups of Comparative Examples 1-3 were administered 0.11 g / kg of the compositions prepared in Comparative Examples 1-3 by gavage, and the positive control group was administered 0.11 g / kg of Zhenju antihypertensive tablets by gavage. The drug administration was carried out for 12 weeks. The blank group (normal group) and the model group (hypertensive group) were administered an equal volume of physiological saline by gavage.

[0056] II. Blood Pressure Measurement After the last administration, the blood pressure of the mice was measured using a non-invasive tail sphygmomanometer. The mice were placed in a mouse restraint device, and an occlusion cuff was placed about 1 cm from the base of the tail, into which a volumetric pressure sensor was inserted. After the mice were calm in the dark for 10 minutes, systolic blood pressure (SBP) and diastolic blood pressure (DBP) were measured, with 3 measurements taken for each mouse.

[0057] III. Establishment of a diabetic mouse model After one week of acclimatization feeding, C57 / J mice were fasted for 12 hours with no water. Ten mice were injected intraperitoneally with saline as a control group, while the remaining mice were injected intraperitoneally twice at 24-hour intervals with freshly prepared alloxan-containing saline solution at 0–4°C to establish a diabetic model. The ratio of the two injection doses was 3:2. Four hours after the first injection, all groups were given free access to 5% glucose to prevent hypoglycemia and subsequent death. After 4 hours, the mice were given regular drinking water. After the second injection, mice were allowed free access to food and water. The average food intake and water intake of each group were measured at fixed times daily. After one week, mice were fasted for 12 hours (with no water restriction), and fasting blood glucose was measured by tail amputation. A blood glucose level greater than 11.1 mmol / L was considered a successful model.

[0058] IV. Grouping and Treatment of Experimental Animals for Lowering Blood Glucose Mice were sorted by blood glucose levels from lowest to highest, and those with blood glucose levels greater than 25 mmol / L and less than 11.1 mmol / L were culled. Each group consisted of 10 mice: a control group, a model group, a positive control group (metformin hydrochloride group, 0.11 g / kg), Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, Example 7 group, Example 8 group, Example 9 group, and Comparative Example 1, Comparative Example 2, and Comparative Example 3. The model and control groups were fed a basal diet; the drug treatment groups were given 0.11 g / kg of the corresponding drug, i.e., Examples 1-9 were given 0.11 g / kg of the compositions I-IX prepared in the above examples, Comparative Examples 1-3 were given 0.11 g / kg of the compositions prepared in Comparative Examples 1-3, and the positive control group was given 0.11 g / kg of metformin hydrochloride. Bedding was changed and water bottles were cleaned daily. After 30 days of continuous feeding, the animals were fasted for 12 hours but allowed free access to water. Blood was then collected from the tail to measure fasting blood glucose levels.

[0059] V. Grouping and Treatment of Experimental Animals for Lowering Blood Lipids After one week of acclimatization feeding, C57 / J mice were randomly divided into 15 groups of 10 mice each: blank group, model group (high-lipid group), positive control group (0.24 g / kg, prepared with physiological saline to a drug concentration of 0.024 g / mL), Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, Example 7 group, Example 8 group, Example 9 group, Comparative Example 1 group, Comparative Example 2 group, and Comparative Example 3 group. The normal group was fed a normal diet, while the other groups were fed a high-fat diet. The drug administration groups were administered 0.01 g / kg of the corresponding drug via gavage. Specifically, groups 1-9 were administered 0.01 g / kg of the compositions I-IX prepared in the above examples via gavage, and groups 1-3 were administered 0.01 g / kg of the compositions prepared in comparative examples 1-3 via gavage. The positive control group was administered 0.24 g / kg of Xuezhikang tablets via gavage. The administration lasted for 12 weeks. The blank group (normal group) and the model group (high-fat group) were administered an equal volume of physiological saline via gavage. After 30 days of continuous feeding, the animals were fasted but allowed free water for 12 hours. Blood was collected from the eyeballs, serum was separated, and the levels of TG, TC, and LDL-C were measured.

[0060] Experimental results 1. Effects on blood pressure changes in hypertensive mice Compared with the blank group, the diastolic and systolic blood pressure of the model group increased significantly (P<0.05). Compared with the model group, the diastolic and systolic blood pressure of the Example 1-9 groups decreased slightly (P<0.05). The blood pressure results show that among the compositions of the Example 1-9 groups, the Example 4 group is superior to the positive control group and the comparative group, as shown in Table 1.

[0061] Table 1 Effects on blood pressure changes in hypertensive mice ( (n=10)

[0062] Note: The subheadings indicate that after analysis of variance - pairwise comparisons, different letters in the same column indicate significant differences (P < 0.05), and the same letters in the same column indicate no significant differences (P > 0.05).

[0063] 2. Effects on blood glucose in mice Compared with the blank group, the blood glucose level in the model group was significantly increased (P<0.05). Compared with the model group, the blood glucose level in the positive drug group and the mice in Examples 1-9 was significantly decreased (P<0.05). The results of blood glucose level show that in Examples 1-9, the mice in Example 4 were better than the mice in the positive drug group and the control group, as shown in Table 2.

[0064] Table 2 Effects on blood glucose in mice ( (n=10)

[0065] Note: The subheadings indicate that after analysis of variance - pairwise comparisons, different letters in the same column indicate significant differences (P < 0.05), and the same letters in the same column indicate no significant differences (P > 0.05).

[0066] 3. Results of the effect on blood lipid levels Compared with the blank group, the levels of TG, TC and LCL-C in the model group were significantly increased (P<0.05). Compared with the model group, the levels of TG, TC and LCL-C in the positive drug group and the examples 1-9 were significantly decreased (P<0.05). The results of blood lipid indicators show that, among the examples 1-9, the example 4 group was better than the positive drug group and the comparative example group, as shown in Table 3.

[0067] Table 3 Results of blood lipid indicators in each group ( (n=10)

[0068] Note: The subheadings indicate that after analysis of variance - pairwise comparisons, different letters in the same column indicate significant differences (P < 0.05), and the same letters in the same column indicate no significant differences (P > 0.05).

[0069] Based on Tables 1-3 above, the compositions of Examples 1-9 provided by the present invention have a significant hypotensive effect on hypertensive mice. As can be seen from the detection of blood glucose and blood lipid levels, they exert the effect of lowering the three highs by regulating lipid metabolism and blood glucose levels. The composition of Example 4 has the best effect and is superior to the positive drug group and other composition groups under the same dosage conditions.

[0070] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A composition for lowering blood lipids, blood sugar, and improving blood pressure, characterized in that, Composed of the following parts by weight of traditional Chinese medicine extract: Chinese herbal extract A: 3-9 parts; Chinese herbal extract B: 2-4 parts; Chinese herbal extract C: 5-7 parts; Chinese herbal extract D: 2-6 parts; The preparation method of traditional Chinese medicine extract A includes the following steps: Gastrodia elata, Rehmannia glutinosa, Cassia tora, and Gardenia jasminoides were mixed, pectinase and water were added, and microwave extraction was performed. The extract was collected and freeze-dried to obtain Chinese herbal extract A. The preparation method of traditional Chinese medicine extract B includes the following steps: Gastrodia elata was extracted using a continuous reflux extraction method. The extract was collected, filtered, concentrated, and freeze-dried to obtain traditional Chinese medicine extract B. The preparation method of traditional Chinese medicine extract C includes the following steps: After mixing Rehmannia glutinosa, Gardenia jasminoides and Cornus officinalis, water ultrasonic extraction was performed. The extract was collected, filtered, concentrated, polyethylene glycol and polydextrose were added, the mixture was vortexed and centrifuged, the supernatant was collected and freeze-dried to obtain Chinese herbal extract C. The preparation method of traditional Chinese medicine extract D includes the following steps: After mixing cassia seeds and gastrodia elata, the mixture was extracted with water multiple times. The extracts were collected, filtered, concentrated, and freeze-dried to obtain herbal extract D.

2. The composition for lowering blood lipids, blood sugar, and improving blood pressure according to claim 1, characterized in that, It is composed of the following parts by weight of traditional Chinese medicine extracts: 6 portions of herbal extract A, 2 portions of herbal extract B, 6 portions of herbal extract C, and 6 portions of herbal extract D.

3. The composition for lowering blood lipids, blood sugar, and improving blood pressure according to claim 1, characterized in that, In the preparation method of traditional Chinese medicine extract A, the mass ratio of Gastrodia elata, Rehmannia glutinosa, Cassia tora, and Gardenia jasminoides is 4:6:8:

4.

4. The composition for lowering blood lipids, blood sugar, and improving blood pressure according to claim 1, characterized in that, In the preparation method of traditional Chinese medicine extract A, the microwave extraction power is 320w and the extraction time is 60min.

5. The composition for lowering blood lipids, blood sugar, and improving blood pressure according to claim 1, characterized in that, In the preparation method of traditional Chinese medicine extract B, the mass fraction of Gastrodia elata is 15 parts.

6. The composition for lowering blood lipids, blood sugar, and improving blood pressure according to claim 1, characterized in that, In the preparation method of Chinese herbal extract B, the number of continuous reflux extractions is 2, and the extraction time is 1.5h each time.

7. The composition for lowering blood lipids, blood sugar, and improving blood pressure according to claim 1, characterized in that, In the preparation method of Chinese herbal extract C, the mass ratio of Rehmannia glutinosa, Gardenia jasminoides and Cornus officinalis is 20:10:10, and the ultrasonic extraction time is 30 min.

8. The composition for lowering blood lipids, blood sugar, and improving blood pressure according to claim 1, characterized in that, In the preparation method of traditional Chinese medicine extract D, the mass ratio of cassia seed and gastrodia is 10:7, the water extraction is performed twice, and the extraction time is 1.5h each time.

9. The use of the composition according to any one of claims 1-8 in the preparation of a medicament for treating high blood lipids, high blood sugar, and high blood pressure.

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