Phycoerythrin PE-1 with fluorescence characteristic, fusion protein of phycoerythrin PE-1 and application of phycoerythrin PE-1

By designing a novel phycoerythrin PE-1 and its fusion protein SA-PE, the limitations of the natural phycoerythrin extraction process have been overcome, achieving efficient fluorescence amplification and accurate detection, which is suitable for solid-phase immunoassay in the biomedical and industrial fields.

CN121494949AActive Publication Date: 2026-02-10OCEAN UNIV OF CHINA
View PDF 3 Cites 0 Cited by

Patent Information

Application Number
CN202610030498.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-01-12
Publication Date
2026-02-10
Estimated Expiration
2046-01-12

AI Technical Summary

Technical Problem

The current extraction process of natural phycoerythrin is affected by the season, algal growth cycle and environmental conditions, resulting in low yield, high cost and great difficulty in purification. Moreover, existing engineering strategies are difficult to break through the original sequence framework, which limits its application in the biomedical and industrial fields.

Method used

A novel phycoerythrin PE-1 and its fusion protein were designed. A recombinant phycoerythrin with a unique structure and function was constructed by designing an amino acid sequence. It was then combined with streptavidin to form the fusion protein SA-PE for solid-phase immunoassay, achieving efficient fluorescence amplification and accurate detection.

Benefits of technology

It improves fluorescence emission capability and thermal stability, enhances signal-to-noise ratio, reduces overlap between absorption and emission peaks, and improves detection accuracy and sensitivity. It is suitable for platforms such as ELISA, immunochromatography, and rapid diagnostic test strips, and has good industrial application value.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN121494949A_ABST
    Figure CN121494949A_ABST
Patent Text Reader

Abstract

The invention discloses phycoerythrin PE-1 with fluorescence characteristics and a fusion protein and application thereof, and belongs to the technical field of gene engineering. The invention provides novel phycoerythrin. The amino acid sequence of the phycoerythrin is shown as SEQ ID NO. 1. The phycoerythrin has high fluorescence quantum yield and fluorescence emission capability. The invention also provides a fusion protein constructed by fusion of phycoerythrin and streptavidin through a connecting peptide, a recombinant expression vector containing a coding sequence of the fusion protein, and a recombinant host cell capable of expressing the fusion protein. The fusion protein provided by the invention can be used for solid-phase immunodetection systems such as ELISA (Enzyme-Linked Immunosorbent Assay), immunochromatography and the like, and sensitive detection on a target object is realized through a 540 / 580nm fluorescence detection mode. The fusion protein provided by the invention has remarkable advantages in the aspects of signal-to-noise ratio and detection stability, and can be widely applied to the field of biomedical detection and diagnosis.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Preparation method of high fluorescence intensity recombinant phycobiliprotein concatermer

    CN106916839A

  • Fusion protein capable of specifically binding to marker and application thereof

    CN121086077A

  • AU1286199A