Application of corn EDS1 gene in resisting southern rust disease

By increasing the expression level of the EDS1 gene or enhancing its protein activity in maize cells, and utilizing the direct interaction between the EDS1 protein and the effector protein Ppz002758 of Southern Rust, the problems of single resistance source and chemical pollution in the control of maize Southern Rust were solved, achieving long-lasting and effective disease resistance and environmentally friendly disease control.

CN121495980APending Publication Date: 2026-02-10CHINA AGRI UNIV
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Patent Information

Application Number
CN202511817404.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-04
Publication Date
2026-02-10

AI Technical Summary

Technical Problem

Existing technologies for controlling maize southern rust have problems such as limited source of resistance, pollution from chemical control, and short-lived resistance, making it difficult to achieve green and long-term disease control.

Method used

By increasing the expression level of the EDS1 gene in maize cells or enhancing its protein activity, and utilizing the direct interaction between the EDS1 protein and the southern rust fungus effector protein Ppz002758, the inhibition of the maize immune response by the rust fungus was blocked. The EDS1 gene was stably expressed in maize using genetic transformation and CRISPR/Cas9 gene editing technology.

Benefits of technology

This has broadened the germplasm base for disease-resistant maize, provided long-lasting and effective disease resistance, avoided environmental pollution caused by chemical control, and achieved diversification of disease-resistant resources and durability of disease resistance.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention relates to the field of plant genetic engineering, and particularly discloses application of a corn EDS1 gene in resisting southern rust. The application is based on the new discovery of direct interaction of southern rust fungus effect protein Ppz002758 and corn EDS1 protein, by constructing an overexpression vector, an editing vector or a co-expression vector and transforming corn, the EDS1 expression level is improved to block rust fungus infection, and new targets and resources are provided for corn disease-resistant breeding. The preparation method comprises the following steps: constructing an expression vector containing the EDS1 gene, transforming a corn immature embryo by an agrobacterium-mediated method, carrying out tissue culture to obtain a regenerated plant, and finally carrying out molecular identification and disease resistance screening to obtain a disease-resistant strain. According to the technical scheme provided by the invention, a new gene resource and a new breeding material are provided for preventing and treating the corn southern rust disease, and the method has the advantages of novel resistant source, environment friendliness and no influence on normal agronomic traits. In addition, the preparation method disclosed by the invention has the advantages of high transformation efficiency and short breeding period.
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