Preparation method of forest musk deer excrement-derived anti-inflammatory and analgesic extract
Anti-inflammatory and analgesic components were extracted from musk deer feces using water and ethanol extraction methods, which solved the problem of underutilization of musk deer feces resources. The prepared extracts showed significant anti-inflammatory and analgesic effects and have good economic value and biosafety.
Patent Information
- Application Number
- CN202511959642.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-12-24
- Publication Date
- 2026-02-17
AI Technical Summary
Musk deer feces resources are not being fully utilized, and there is a lack of existing technologies to effectively extract and apply the active ingredients from musk deer feces.
Anti-inflammatory and/or analgesic components were extracted from musk deer feces using water extraction and/or ethanol extraction methods. The musk deer feces extract was prepared by heating and reflux extraction and mixing with commonly used pharmaceutical excipients. The product was then obtained after drying.
The prepared musk deer fecal extract exhibited anti-inflammatory effects comparable to dexamethasone and analgesic effects comparable to aspirin. It is also easy to prepare, has good biocompatibility, and is inexpensive, thus showing broad application prospects.
Smart Images
Figure FT_1 
Figure FT_2 
Figure FT_3
Abstract
Description
Technical Field
[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a method for preparing an anti-inflammatory and analgesic extract derived from musk deer feces. Background Technology
[0002] Forest musk deer ( Moschus berezovskii The forest musk deer (Flerov) is a small deer species that typically inhabits mountainous forests, feeding on leaves, twigs, fruits, and herbs. While its feces have some applications in traditional medicine, their use is relatively limited, resulting in the underutilization and waste of this resource. Therefore, how to comprehensively utilize forest musk deer feces has become one of the current research topics for forest musk deer.
[0003] Currently, the application of musk deer feces is mainly concentrated in folk remedies or certain traditional Chinese medicine prescriptions. In traditional medicine, musk deer feces are called "musk feces" or "musk excrement." Some folk remedies believe that after processing, it can be applied externally to relieve rheumatic pain; "dried feces" are used for sores or insect and snake bites; and "after processing and combining with other medicinal materials," it can improve abdominal distension and indigestion, thus being considered to have certain medicinal value. However, there are no reported techniques for effectively extracting and applying the active ingredients from musk deer feces. Summary of the Invention
[0004] To address the problems of insufficient utilization of musk deer feces resources and inadequate efficacy of musk deer feces in existing technologies, this invention provides a method for preparing an anti-inflammatory and analgesic extract derived from musk deer feces, specifically including the following technical solution: The present invention provides an anti-inflammatory and / or analgesic product, wherein the active ingredient of the product includes a musk deer fecal extract.
[0005] Preferably, the extract includes an aqueous extract and / or an ethanol extract.
[0006] Preferably, the ethanol extract is an extract obtained from ethanol, wherein the volume percentage of ethanol is 0.1% to 100%.
[0007] Preferably, the product includes pharmaceuticals.
[0008] Preferably, the ingredients of the drug also include other pharmaceutically commonly used excipients.
[0009] The present invention also provides a method for preparing the anti-inflammatory and / or analgesic product as described above, comprising the following steps: The musk deer feces and solvent were mixed at a ratio of 1 g: 20-30 mL to obtain a mixture; The mixture was heated and refluxed for extraction to obtain a musk deer fecal extract; The solvent includes water and / or ethanol; The product is prepared by mixing the musk deer feces extract with excipients.
[0010] Preferably, the hot reflux extraction conditions are 85~95℃ for 2~4 hours.
[0011] Preferably, after obtaining the musk deer fecal extract, the method further includes drying the musk deer fecal extract to obtain a dried extract; The drying process includes vacuum drying or freeze drying.
[0012] Preferably, the excipients include pharmaceutically common excipients.
[0013] The present invention also provides the use of products prepared by the methods described above in the preparation of anti-inflammatory and / or analgesic pharmaceutical products.
[0014] The beneficial effects of this invention are as follows: This invention provides an anti-inflammatory and / or analgesic product, the active ingredient of which includes a fecal extract of the musk deer. This invention is the first to experimentally verify the anti-inflammatory and analgesic effects of the musk deer fecal extract, with anti-inflammatory effects comparable to dexamethasone and analgesic effects comparable to aspirin. Furthermore, the musk deer fecal extract prepared by this invention is easy to prepare, biosafety-free, and inexpensive, making it convenient to prepare and use, and possessing broad application prospects and good economic value. Attached Figure Description
[0015] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.
[0016] Figure 1 Effects of ethanol extracts and water extracts prepared from ethanol of different concentrations on the cytotoxicity and NO production of RAW 264.7 cells; Figure 2 The effects of extracts at different concentration gradients on the cytotoxicity and NO production of RAW 264.7 cells; Figure 3 The effects of extracts at different concentration gradients on the expression of inflammatory factors TNF-α, IL-1β and IL-6 mRNA in RAW 264.7 cells were investigated. Figure 4 The effects of extracts at different concentration gradients on a mouse model of pain induced by glacial acetic acid; Figure 5 The effect of extracts at different concentration gradients on xylene-induced ear swelling in mice; Figure 6 The effect of extracts at different concentration gradients on the degree of inflammatory infiltration in xylene-induced ear swelling in mice; Figure 7The effects of extracts at different concentration gradients on biochemical indicators of TNF-α, IL-1β, and IL-6 inflammatory stress in xylene-induced mouse ear swelling; Figures 1-7 middle, Indicates comparison with the model group P< 0.05, Indicates comparison with the model group P< 0.01, ## indicates that the model group is compared with the blank group. P< 0.01. Detailed Implementation
[0017] The present invention provides an anti-inflammatory and / or analgesic product, wherein the active ingredient of the product includes a musk deer fecal extract.
[0018] In one embodiment, the extract includes an aqueous extract and / or an ethanol extract. In one embodiment, the ethanol extract is an extract derived from ethanol. In one embodiment, the volume percentage of ethanol is 0.1% to 100%. In one embodiment, the volume percentage of ethanol can be any one or an intermediate value of any two points from 0.1%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. In one embodiment, the product includes a pharmaceutical product. In one embodiment, the pharmaceutical product also includes other pharmaceutically commonly used excipients. In one embodiment, the excipients include any one or more of solvents, solubilizers, co-solvents, antibacterial agents, suspending agents, and emulsifiers.
[0019] The musk deer fecal extract described in this invention has good anti-inflammatory and analgesic effects. Cell experiments and animal experiments have confirmed that the musk deer fecal extract prepared by this invention has good therapeutic effects on acetic acid-induced mouse pain models and xylene-induced mouse ear swelling models. It can significantly alleviate the degree of pain induced by acetic acid in mice; it can also significantly alleviate the degree of inflammatory infiltration in the ear tissue of mice with xylene-induced ear swelling; and it can regulate the inflammatory stress biochemical indicators in the mouse ear tissue.
[0020] The present invention also provides a method for preparing the anti-inflammatory and / or analgesic product as described above, comprising the following steps: The musk deer feces and solvent were mixed at a ratio of 1 g: 20-30 mL to obtain a mixture; The mixture was heated and refluxed for extraction to obtain a musk deer fecal extract; The solvent includes water and / or ethanol; The product is prepared by mixing the musk deer feces extract with excipients.
[0021] This invention involves mixing musk deer feces and a solvent at a material-to-liquid ratio of 1 g: 20-30 mL to obtain a mixture. In one embodiment, the solvent includes water and / or ethanol. In one embodiment, the water includes RO water. In one embodiment, the volume percentage of ethanol is 0.1% to 100%. In one embodiment, the volume percentage of ethanol can be any one or any intermediate value of any two points from 0.1%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. In a preferred embodiment, the volume percentage of ethanol is 60-100%. In a more preferred embodiment, the volume percentage of ethanol is 80%. In one embodiment, the ratio of musk deer feces to 80% ethanol is 15 g: 400 mL.
[0022] After obtaining the mixture, the present invention heats the mixture under reflux to extract musk deer feces extract. As one embodiment, the temperature of the reflux extraction is 85-95°C. As another embodiment, the reflux extraction temperature can be any one or any two values between 85°C, 86°C, 87°C, 88°C, 89°C, 90°C, 91°C, 92°C, 93°C, 94°C, and 95°C. As another embodiment, the reflux extraction time is any one or any two values between 2 h, 3 h, and 4 h. In a specific embodiment, the reflux extraction conditions are 90°C for 3 h.
[0023] In one embodiment, after obtaining the musk deer fecal extract, the process further includes drying the musk deer fecal extract to obtain a dried extract. In one embodiment, the drying includes vacuum drying or freeze drying. In one embodiment, the vacuum drying time is 5 hours, and the vacuum drying temperature is 60°C.
[0024] This invention involves mixing the musk deer feces extract with excipients to obtain the product. As one embodiment, the excipients include pharmaceutically common excipients. The types of excipients are as previously described and will not be repeated here.
[0025] The present invention also provides the use of the product or product obtained by the said method in the preparation of anti-inflammatory and / or analgesic pharmaceutical products.
[0026] To further illustrate the present invention, the preparation method of an anti-inflammatory and analgesic extract from musk deer feces provided by the present invention will be described in detail below with reference to the accompanying drawings and embodiments, but these should not be construed as limiting the scope of protection of the present invention.
[0027] Example 1: Preparation method of musk deer fecal extract Taking the 80% ethanol extract as an example, musk deer feces were mixed with 80% ethanol solution at a material-to-liquid ratio of 15 g: 400 mL. After obtaining the mixture, it was heated and refluxed at 90℃ for 3 h to obtain the 80% ethanol extract of musk deer feces.
[0028] The dried extract was obtained by vacuum drying the 80% ethanol extract of musk deer feces at 60℃ for 5 h.
[0029] Example 2: Effects of different musk deer fecal extracts on NO content in LPS-induced RAW 264.7 cells 1) Experimental method: Preparation of extracts with different ethanol concentrations: Musk deer feces extract was prepared according to the method described in Example 1, except that the volume percentage of the ethanol solution used in the preparation process was 20%, 40%, 60%, 80%, or 100%, respectively. Dried extracts of 20%, 40%, 60%, 80%, and 100% ethanol were obtained, respectively.
[0030] Preparation of water extract from musk deer feces: Musk deer feces were mixed with RO water at a material-to-liquid ratio of 15 g: 400 mL. The mixture was then subjected to reflux extraction at 90°C for 3 h to obtain the water extract. The water extract was then vacuum dried at 60°C for 5 h to obtain the dried extract.
[0031] The dried extracts of the prepared musk deer feces water extract, 20% ethanol extract, 40% ethanol extract, 60% ethanol extract, 80% ethanol extract and 100% ethanol extract were stored at -20℃ for a long period of time.
[0032] Stock solution of musk deer feces extract: Accurately weigh 25 mg of the dried extracts of each of the above-obtained extracts and dissolve them in 1 mL of dimethyl sulfoxide (cell culture grade). Sonicate for 30 min to prepare stock solution. Use stock solution to prepare the concentration of each extract required for subsequent cell experiments.
[0033] Dexamethasone stock solution: Accurately weigh 25 mg of 99% pure dexamethasone powder and dissolve it in 1 mL of dimethyl sulfoxide (cell culture grade). Sonicate for 30 min to obtain the stock solution. Use the stock solution to prepare the required dexamethasone concentration.
[0034] Experimental group settings: Control group: The control group contains only basal culture medium, without LPS or any drug interference.
[0035] Model group: The model group contains only LPS with a final concentration of 1 μg / mL without any drug interference.
[0036] Positive control group (DXMS): Intervention was performed using LPS at a final concentration of 1 μg / mL and dexamethasone at a final concentration of 0.1 mg / mL.
[0037] 20%, 40%, 60%, 80% and 100% ethanol extract groups: The stock solutions of each ethanol extract were diluted to 0.1 mg / mL, 0.05 mg / mL and 0.01 mg / mL, respectively. Each extract group was treated with extracts at a final concentration of 1 μg / mL LPS and final concentrations of 0.1 mg / mL, 0.05 mg / mL and 0.01 mg / mL, respectively.
[0038] Water extract group: Water extract stock solution was diluted to 0.1 mg / mL, 0.05 mg / mL, and 0.01 mg / mL, and water extracts containing a final concentration of 1 μg / mL LPS and final concentrations of 0.1 mg / mL, 0.05 mg / mL, and 0.01 mg / mL were used for intervention.
[0039] The preparation methods of the drugs used in each group, the cell culture methods, the cell viability assay methods, and the method for detecting changes in NO content in RAW 267.4 cells induced by LPS were all referenced from the literature "Fu YJ, et al. Baicalinprevents LPS-induced activation of TLR4 / NF-κB p65 pathway and inflammation inmice via inhibiting the expression of CD14. Acta Pharmacol Sin. 2021 Jan;42(1):88-96." The experimental results are as follows: Figure 1 As shown.
[0040] 2) Experimental results: Figure 1 Figure A shows that the five kinds of dried musk deer fecal ethanol extracts and musk deer fecal water extracts all showed a survival rate of more than 90% for RAW 264.7 cells at drug doses of 0.1 mg / mL, 0.05 mg / mL, and 0.01 mg / mL, with no obvious toxic side effects, proving that the dried musk deer fecal extracts prepared by the method of the present invention are safe.
[0041] Figure 1 The results showed that LPS stimulation of RAW 264.7 cells for 24 h significantly upregulated NO levels, while the five groups of musk deer fecal ethanol extract, musk deer fecal water extract, and dexamethasone group significantly downregulated the LPS-upregulated NO levels. PThe concentration of NO in the extract was <0.01%, indicating that both the ethanol and water extracts of musk deer feces can inhibit NO production to some extent. The effect was: 80% ethanol extract > 60% ethanol extract > 100% ethanol extract > 40% ethanol extract > 20% ethanol extract > water extract. Based on these results, 60%–100% ethanol is preferred as the extraction solvent, and 80% ethanol is more preferred. Subsequent examples all used the 80% ethanol extract as the sample for experiments.
[0042] Example 3: Effects of different concentrations of musk deer fecal extract on LPS-induced NO content in RAW 264.7 cells 1) Experimental method: Stock solution of musk deer feces extract: Accurately weigh 25 mg of dried powder of musk deer feces extract, dissolve it in 1 mL of dimethyl sulfoxide (cell culture grade), and sonicate for 30 min to obtain stock solution. Use stock solution to prepare the concentration required for cell experiments.
[0043] Dexamethasone stock solution: Accurately weigh 25 mg of 99% pure dexamethasone powder, dissolve it in 1 mL of dimethyl sulfoxide (cell culture grade), and sonicate for 30 min to obtain dexamethasone stock solution. Use the dexamethasone stock solution to prepare the required dexamethasone concentration.
[0044] The methods for cell culture, cell viability assay, and detection of NO content changes in RAW 267.4 cells induced by LPS were also as described in Example 2, in the literature "Fu YJ, et al. 2021". The experimental results are as follows: Figure 2 As shown.
[0045] Specifically, the concentration of dexamethasone stock solution was prepared to 0.1 mg / mL using DMEM high-glucose medium as a positive control sample; the concentration of musk deer fecal extract was diluted to 0.2 mg / mL, 0.1 mg / mL, 0.05 mg / mL, 0.025 mg / mL, 0.0125 mg / mL, and 0.00625 mg / mL using DMEM high-glucose medium as administration samples.
[0046] 2) Experimental results: Depend on Figure 2 As shown in Figure A, the survival rate of RAW 264.7 cells was greater than 90% at drug doses of 0.2 mg / mL, 0.1 mg / mL, 0.05 mg / mL, 0.025 mg / mL, 0.0125 mg / mL, and 0.00625 mg / mL, indicating that the extract had no obvious toxic side effects.
[0047] Depend on Figure 2 As shown in Figure B, the NO content in all treatment groups and the positive drug group was significantly lower than that in the model group.P <0.01), and the extracts at concentrations of 0.2 mg / mL, 0.1 mg / mL, 0.05 mg / mL, and 0.025 mg / mL showed better inhibitory effects on NO than the positive control drug dexamethasone. This demonstrates that the extracts at all six concentration gradients can inhibit NO production to some extent; the effect is: 0.1 mg / mL > 0.2 mg / mL > 0.05 mg / mL > 0.025 mg / mL > 0.0125 mg / mL > 0.00625 mg / mL. Therefore, in subsequent examples, three final concentrations of 0.025–0.1 mg / mL were selected as the drug administration concentrations for RAW 264.7 cells.
[0048] Example 4: Effects of different concentrations of musk deer fecal extract on the expression of LPS-induced inflammatory factors TNF-α, IL-1β, and IL-6 mRNA in RAW 264.7 cells. 1) Experimental method: The preparation methods for the musk deer fecal extract mother liquor, dexamethasone mother liquor, and dexamethasone positive control sample were the same as in Example 3. The extracts were diluted to concentrations of 0.1 mg / mL, 0.05 mg / mL, and 0.025 mg / mL using DMEM high-glucose medium to prepare the drug-treated samples.
[0049] The cell culture, cell viability assay, and detection methods for LPS-induced changes in the expression of inflammatory cytokines mRNA in RAW 264.7 cells using Real-time quantitative PCR were referenced from "Dong X, et al. Nuanxinkang protects against ischemia / reperfusion-induced heart failure through regulating IKKβ / IκBα / NF-κB-mediated macrophage polarization. Phytomedicine. 2022 Jul;101:154093." The results are as follows: Figure 3 As shown.
[0050] 2) Experimental results: Depend on Figure 3 It was found that the mRNA levels of TNF-α, IL-1β, and IL-6 in the model group cells were significantly higher than those in the control group; compared with the model group, the mRNA levels of TNF-α, IL-1β, and IL-6 in each drug-treated group were significantly reduced, and the inhibitory effect of the extract on IL-1β was significantly stronger than that in the positive control group. P <0.05 or P<0.01), the inhibitory effect of the extract on TNF-α and IL-6 mRNA was comparable to that of the positive control group. It can be seen that the 0.025~0.1 mg / mL forest musk deer fecal extract prepared in this invention can significantly reduce the expression level of inflammatory factors and produce an ideal anti-inflammatory effect.
[0051] Example 5: Effects of different concentrations of musk deer fecal extract on an acetic acid-induced mouse pain model. 1) Experimental Objective This study used mice with acetic acid-induced pain as the research subject. The modeling method for acetic acid-induced pain mice was as follows: freshly prepared 0.6% acetic acid was injected into the peritoneum of mice at an injection dose of 0.01 mL / g.
[0052] The number of writhing movements (extending hind limbs, abdominal concavity, and raising hips) within 20 minutes after acetic acid-induced pain in mice was used as the observation index, and aspirin was used as the positive control drug to analyze the analgesic effect of different concentrations of musk deer fecal extract.
[0053] 2) Experimental Grouping Forty SPF-grade ICR mice aged 6-8 weeks were selected, with half males and half females. After acclimatization for 7 days, they were randomly divided into 5 groups, with 4 females and 4 males in each group. The 5 groups of mice were designated as: model group (control group), positive control group (aspirin group), high-dose group of musk deer fecal extract (100 mg / kg), medium-dose group of musk deer fecal extract (50 mg / kg), and low-dose group of musk deer fecal extract (25 mg / kg).
[0054] 3) Experimental methods Following the grouping treatments described in step 2), the model group was administered physiological saline by gavage. The high-dose, medium-dose, and low-dose groups were administered 100 mg / kg / day, 50 mg / kg / day, and 25 mg / kg / day of musk deer fecal extract solution by gavage, respectively. The positive control group was administered 200 mg / kg / day of aspirin solution by gavage. All groups were administered the drug at a dose of 0.1 mL / 10g body weight (using physiological saline as the carrier solvent). Gavage was continued for 7 days, once daily at a fixed time. Sixty minutes after the last administration, all mice were intraperitoneally injected with 0.01 mL / g of freshly prepared 0.6% glacial acetic acid. The number of writhing movements within 20 minutes after injection was used as the observation index. After the observation period, the mice were anesthetized and euthanized.
[0055] 4) Experimental Indicators When stimulated with 0.6% glacial acetic acid, blinded observers were asked to count the number of twisting movements (extending hind limbs, abdominal concavity, and raising hips).
[0056] 5) Statistical methods All experimental data are expressed as mean ± standard deviation and statistically analyzed using GraphPad Prism 8; one-way ANOVA was used for comparisons among multiple groups. P <0.05 indicates a statistically significant difference.
[0057] 6) Experimental Results 6.1 Results of the acetic acid-induced mouse pain model The results of the number of writhing movements in mice after acetic acid stimulation are as follows: Figure 4 As shown, compared with the model group, the number of writhing movements in the positive drug group and the different doses of extract group decreased significantly within 20 minutes. P <0.05 or P <0.01), and both the medium-dose and high-dose groups were more effective than the positive control group.
[0058] 6.2 Summary Animal experiments have shown that the extract obtained from the feces of musk deer in this invention has a certain ameliorative effect on the acetic acid-induced mouse pain model. The medium-dose group and the high-dose group showed better results than the positive drug group in terms of behavioral scores.
[0059] Example 6: Experimental Verification of the Therapeutic Effect of Musk Deer Fecal Extract on Xylene-Induced Ear Swelling in Mouse Ear Tissue 1) Experimental Objective This study used a xylene-induced ear swelling model in mice. Sixty minutes after the last oral administration of xylene, 20 μL of xylene was applied to both the front and back of the right ear. The degree of ear swelling, pathological observation, and expression levels of cell-related inflammatory factors were used as observation indicators. Dexamethasone was used as a positive control to analyze the pharmacodynamic effects of musk deer fecal extract in treating inflammation.
[0060] 2) Experimental Grouping Forty-eight SPF-grade ICR mice aged 6-8 weeks were selected, with half males and half females. After 7 days of acclimatization, they were randomly divided into 6 groups, with 4 females and 4 males in each group. These groups were designated as: blank group, model group, positive control group (2 mg / kg dexamethasone group), high-dose group of musk deer fecal extract (100 mg / kg), medium-dose group of musk deer fecal extract (50 mg / kg), and low-dose group of musk deer fecal extract (25 mg / kg).
[0061] 3) Experimental methods Following the grouping and treatment described in step 2), the blank control group and model group were administered physiological saline by gavage. The high-dose group, medium-dose group, and low-dose group were administered 100 mg / kg / d, 50 mg / kg / d, and 25 mg / kg / d of musk deer fecal extract solution by gavage, respectively. The positive control group was administered 2 mg / kg / d of dexamethasone solution by gavage. All groups were administered 0.1 mL / 10 g body weight (using physiological saline as the carrier solvent). Gavage was continued for 7 days, once daily at a fixed time. Sixty minutes after the last administration, except for the blank control group, 20 μL of xylene was applied to both sides of the right ear of all mice, with the left ear serving as a control. Thirty minutes after application, the mice were anesthetized and euthanized, and samples were taken from the same location in both ears using an 8 mm punch.
[0062] 4) Experimental Indicators The weight difference of 8 mm ear pieces at the same location in the left and right ears of the same mouse was used as a behavioral observation indicator; the ear pieces were stained with hematoxylin and eosin (H&E staining) to observe the pathological changes in ear tissue; the biochemical indicators of ear tissue inflammation were measured by enzyme-linked immunosorbent assay, and the contents of TNF-α, IL-6 and IL-1β were measured according to the instructions of each kit.
[0063] 5) Statistical methods All experimental data are expressed as mean ± standard deviation and statistically analyzed using GraphPad Prism 8; one-way ANOVA was used for comparisons among multiple groups. P <0.05 indicates a statistically significant difference.
[0064] 6) Experimental Results 6.1 Behavioral performance of mice Depend on Figure 5 As can be seen, the swelling degree in the model group was higher than that in the blank group, and the differences were statistically significant, indicating that this embodiment successfully constructed a mouse model of ear swelling. The ear swelling degree in the positive drug group and the groups containing different doses of musk deer fecal extract was significantly reduced compared to the model group, and the differences were statistically significant. Furthermore, the high-dose group of musk deer fecal extract was more effective than the positive drug group, demonstrating that the musk deer fecal extract prepared in this invention has a good anti-swelling effect.
[0065] 6.2 Effects on inflammatory responses in mouse ear tissues of each group Depend on Figure 6As can be seen, the ear tissue of mice in the blank group was intact, with neatly arranged cells and intact, continuous, and narrow striated muscle fibers. In contrast, the cell arrangement in the model group was less neat, with significant inflammatory cell infiltration and obvious swelling. Compared with the model group, the ear tissue of mice in different concentrations of musk deer fecal extract and the positive control group showed reduced inflammatory cell infiltration, relatively neat mucosal tissue arrangement, and reduced mucosal swelling. Furthermore, in the low, medium, and high dose groups of musk deer fecal extract, the above-mentioned improvement effect was more significant with increasing drug dosage. Therefore, the musk deer fecal extract prepared in this invention can significantly reduce the degree of inflammatory infiltration in ear tissue induced by xylene.
[0066] 6.3 Expression of relevant inflammatory factors in ear tissues of mice in each group Depend on Figure 7 It can be seen that the protein levels of inflammatory factors TNF-α, IL-1β, and IL-6 in the model group mice were significantly higher than those in the blank group. P <0.01); compared with the model group, the levels of inflammatory factors TNF-α, IL-1β, and IL-6 in each musk deer fecal extract group and the positive drug group were significantly reduced ( P <0.01).
[0067] In summary, animal experiments showed that the musk deer fecal extract prepared in this invention significantly improved the xylene-induced ear swelling mouse model. It inhibited the inflammatory response by improving ear tissue inflammatory infiltration and regulating the expression of local inflammatory factors in the ear tissue. The high-dose group showed comparable efficacy to the positive control group in behavioral scores, ear tissue pathology, and reduction of inflammatory biochemical indicators such as TNF-α, IL-1β, and IL-6. This invention utilizes musk deer feces to prepare an extract with effective anti-inflammatory and / or analgesic properties, fully leveraging the medicinal value of musk deer feces.
[0068] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, not all embodiments. People can obtain other embodiments without creative effort, as shown in these embodiments, and these embodiments all fall within the protection scope of the present invention.
Claims
1. An anti-inflammatory and / or analgesic product, characterized in that, The active ingredient in the product includes extract from musk deer feces.
2. The product as described in claim 1, characterized in that, The extracts include water extracts and / or ethanol extracts.
3. The product as described in claim 2, characterized in that, The ethanol extract is an extract obtained from ethanol, wherein the volume percentage of ethanol is 0.1% to 100%.
4. The product as described in claim 1, characterized in that, The products include pharmaceuticals.
5. The product as described in claim 4, characterized in that, The ingredients of the drug also include other pharmaceutically commonly used excipients.
6. A method for preparing the anti-inflammatory and / or analgesic product according to any one of claims 1 to 5, characterized in that, Includes the following steps: The musk deer feces and solvent were mixed at a ratio of 1 g: 20-30 mL to obtain a mixture; The mixture was heated and refluxed for extraction to obtain a musk deer fecal extract; The solvent includes water and / or ethanol; The product is prepared by mixing the musk deer feces extract with excipients.
7. The preparation method according to claim 6, characterized in that, The hot reflux extraction conditions are 85~95℃ for 2~4 hours.
8. The preparation method according to claim 6, characterized in that, After obtaining the musk deer fecal extract, the process also includes drying the musk deer fecal extract to obtain a dried extract; The drying process includes vacuum drying or freeze drying.
9. The preparation method according to claim 6, characterized in that, The excipients include those commonly used in pharmaceutical science.
10. The use of the product according to any one of claims 1 to 5 or the product obtained by the method according to any one of claims 6 to 9 in the preparation of anti-inflammatory and / or analgesic pharmaceutical products.