Whitening composition containing astaxanthin and preparation method thereof

Abstract

The invention discloses a whitening composition containing astaxanthin and a preparation method thereof, and belongs to the technical field of food. The astaxanthin oil composition is prepared from the following components in parts by mass: 20.7 to 40.7 parts of astaxanthin oil, 45 to 65 parts of grape seed oil, 5 to 7 parts of gamma-aminobutyric acid, 8 to 10 parts of purified water, 5 to 7 parts of South African drunken eggplant extract, 2 to 4 parts of black ginger extract, 20 to 28 parts of curcumin lecithin compound, 1.4 to 1.8 parts of grape seed extract, 0.1 to 0.3 part of white tomato extract, 0.6 to 1 part of radix polygonati officinalis extract and 0.6 to 0.8 part of folium mori extract. The whitening composition disclosed by the invention has a good melanin inhibition effect.

Description

Technical Field

[0001] This invention belongs to the field of food technology, specifically, it relates to a whitening composition containing astaxanthin and its preparation method. Background Technology

[0002] With social development and people's yearning and pursuit of a better life, beauty has become an increasingly important pursuit in life, and skin whitening is an important goal of becoming more beautiful.

[0003] Tyrosinase plays a crucial role in melanin formation. Tyrosinase (TYR) is a copper-containing metalloenzyme with a highly conserved double copper-binding region. Each copper ion is linked to three histidine residues via coordinate bonds, forming an active site with a specific structure. The binding of hydrogen peroxide to the two copper ions allows TYR to exist in three different states: oxidative, reduced, and deoxygenated, thus exerting its catalytic effect. TYR plays a vital role in pigment production. It is the rate-limiting enzyme in melanin synthesis, and inhibiting TYR activity is important for the adjunctive treatment of melanoma and most types of pigmentation, which has also promoted the development of skin-whitening cosmetics.

[0004] In the field of skin-whitening cosmetics, commonly used ingredients that can inhibit tyrosinase activity include kojic acid, arbutin, vitamin C, cycloheptatrienolone, and hydroquinone. However, most of these skin-whitening additives have certain side effects. For example, arbutin may hydrolyze into hydroquinone in an acidic environment or under the action of skin microorganisms (such as Staphylococcus aureus). Hydroquinone is a potent skin-whitening ingredient, but it is irritating, sensitizing, and has potential phototoxicity. Long-term use at high concentrations may cause contact dermatitis or abnormal pigmentation. Although kojic acid has a strong inhibitory effect on tyrosinase, it is unstable and long-term use may be carcinogenic. Vitamin C and its derivatives have poor skin absorption and are only effective at high concentrations. Summary of the Invention

[0005] In order to overcome the problems existing in the prior art, the present invention proposes a whitening composition containing astaxanthin and its preparation method.

[0006] To achieve the above objectives, the present invention is implemented through the following technical solution: The first aspect of this invention provides a whitening composition containing astaxanthin, comprising the following components in parts by weight: 20.7-40.7 parts astaxanthin oil, 45-65 parts grape seed oil, 5-7 parts γ-aminobutyric acid, 8-10 parts purified water, 5-7 parts Ashwagandha extract, 2-4 parts black ginger extract, 20-28 parts curcumin-lecithin complex, 1.4-1.8 parts grape seed extract, 0.1-0.3 parts white tomato extract, 0.6-1 part Solomon's seal extract, and 0.6-0.8 parts mulberry leaf extract.

[0007] Furthermore, the preparation method of the curcumin-lecithin complex is as follows: curcumin and soybean lecithin are dissolved in anhydrous ethanol at a mass ratio of 1:2-4, stirred in a water bath at 50°C for 1-1.5 h, and then the ethanol is removed by vacuum distillation. The solid is collected and dried under vacuum for 12-14 h to obtain the curcumin-lecithin complex.

[0008] A second aspect of the present invention provides a method for preparing the astaxanthin-containing whitening composition of the first aspect, comprising the following steps: S1. Mix astaxanthin oil, grapeseed oil and curcumin lecithin, stir well to obtain the oil phase; S2, add γ-aminobutyric acid, grape seed extract, ashwagandha extract, black ginger extract, white tomato extract, Solomon's seal extract and mulberry leaf extract to purified water and stir to disperse evenly to obtain an aqueous phase; S3. Mix the aqueous phase and oil phase, stir and disperse evenly to obtain a suspension. The suspension is first treated with a high-speed dispersion homogenizer, and then treated with an ultrasonic cell disruptor. When using the high-speed dispersion homogenizer: high-speed shear dispersion at 10000r / min-12000r / min for 9-15min. When using the ultrasonic cell disruptor: ultrasonic dispersion at 100W power for 2min, followed by ultrasonic dispersion at 200W power for 4min, selecting the 1s working and 1s intermittent mode to obtain the whitening composition.

[0009] A third aspect of the present invention provides a capsule containing the whitening composition of the first aspect, wherein the capsule contains the whitening composition of the first aspect, and the capsule wall comprises the following components in weight fractions: 38.6% gelatin, 38.6% purified water, and 22.8% glycerin. Through the above technical solution, the present invention can achieve at least the following beneficial effects: the whitening composition of the present invention has a good melanin inhibition effect. Detailed Implementation

[0010] Unless otherwise stated, all materials and reagents used in this invention are commercially available.

[0011] In this invention: Astaxanthin oil and purified water are extracted / prepared by our company (Yunnan Green A Biotechnology Co., Ltd.). The astaxanthin oil contains 5% astaxanthin.

[0012] The grape seed oil was purchased from Heze Zhonghe Jianyuan Biotechnology Co., Ltd. Gamma-aminobutyric acid was purchased from Shandong Kangyou Biotechnology Co., Ltd., and passed through an 80-mesh sieve. Grape seed extract was purchased from Bozhou Xier Traditional Chinese Medicine Co., Ltd. Curcumin was purchased from Lanzhou Waterles Biotechnology Co., Ltd. Soy lecithin was purchased from Guangzhou Mingtong Biotechnology Co., Ltd. The Ashwagandha extract from South Africa was purchased from Shaanxi Qingmiao Biotechnology Co., Ltd. (it is a water extract, and the specifications are all 20:1). Black ginger extract was purchased from Xizhuorui Biotechnology Co., Ltd. (it is a water extract, and the specifications are all 20:1). White tomato extract was purchased from Glide Technology (Shanghai) Co., Ltd. The Polygonatum odoratum extract and mulberry leaf extract were purchased from Shanyang Lianfeng Biotechnology Co., Ltd. (both are water extracts, with a specification of 20:1). The gelatin was purchased from Kunming Sigui Trade Co., Ltd. Glycerin was purchased from Jiangxi Ipsen Pharmaceutical Co., Ltd. Example 1

[0013] A whitening composition containing astaxanthin comprises the following components in parts by weight: 30 parts astaxanthin oil, 55 parts grape seed oil, 6 parts γ-aminobutyric acid, 9 parts purified water, 6 parts Ashwagandha extract, 3 parts black ginger extract, 24 parts curcumin lecithin complex, 1.6 parts grape seed extract, 0.2 parts white tomato extract, 0.8 parts Solomon's seal extract, and 0.7 parts mulberry leaf extract.

[0014] The preparation method of the curcumin-lecithin complex is as follows: curcumin and soybean lecithin are dissolved in anhydrous ethanol at a mass ratio of 1:3, stirred in a water bath at 50°C for 1.2 h, and then the ethanol is removed by vacuum distillation. The solid is collected and dried under vacuum for 13 h to obtain the curcumin-lecithin complex.

[0015] The preparation method of the whitening composition containing astaxanthin includes the following steps: S1. Mix astaxanthin oil, grapeseed oil and curcumin lecithin, stir well to obtain the oil phase; S2, add γ-aminobutyric acid, grape seed extract, ashwagandha extract, black ginger extract, white tomato extract, Solomon's seal extract and mulberry leaf extract to purified water and stir to disperse evenly to obtain an aqueous phase; S3. Mix the aqueous phase and oil phase, stir and disperse evenly to obtain a suspension. The suspension is first treated with a high-speed dispersion homogenizer, and then treated with an ultrasonic cell disruptor. When using the high-speed dispersion homogenizer: high-speed shear dispersion at 11000r / min for 12min. When using the ultrasonic cell disruptor: ultrasonic dispersion at 100W power for 2min, followed by ultrasonic dispersion at 200W power for 4min, selecting the 1s working and 1s intermittent mode, to obtain the whitening composition. Example 2

[0016] A skin-whitening composition containing astaxanthin comprises the following components in parts by weight: 20.7 parts astaxanthin oil, 45 parts grape seed oil, 5 parts γ-aminobutyric acid, 8 parts purified water, 5 parts Ashwagandha extract, 2 parts black ginger extract, 20 parts curcumin-lecithin complex, 1.4 parts grape seed extract, 0.1 parts white tomato extract, 0.6 parts Solomon's seal extract, and 0.6 parts mulberry leaf extract.

[0017] The preparation method of the curcumin-lecithin complex is as follows: curcumin and soybean lecithin are dissolved in anhydrous ethanol at a mass ratio of 1:2, stirred in a water bath at 50°C for 1 hour, and then the ethanol is removed by vacuum distillation. The solid is collected and dried under vacuum for 12 hours to obtain the curcumin-lecithin complex.

[0018] The preparation method of the whitening composition containing astaxanthin includes the following steps: S1. Mix astaxanthin oil, grapeseed oil and curcumin lecithin, stir well to obtain the oil phase; S2, add γ-aminobutyric acid, grape seed extract, ashwagandha extract, black ginger extract, white tomato extract, Solomon's seal extract and mulberry leaf extract to purified water and stir to disperse evenly to obtain an aqueous phase; S3. Mix the aqueous phase and oil phase, stir and disperse evenly to obtain a suspension. The suspension is first treated with a high-speed dispersion homogenizer, and then treated with an ultrasonic cell disruptor. When using the high-speed dispersion homogenizer: high-speed shear dispersion at 10000r / min for 9min. When using the ultrasonic cell disruptor: ultrasonic dispersion at 100W power for 2min, followed by ultrasonic dispersion at 200W power for 4min, selecting the 1s working and 1s intermittent mode to obtain the whitening composition. Example 3

[0019] A skin-whitening composition containing astaxanthin comprises the following components in parts by weight: 40.7 parts astaxanthin oil, 65 parts grape seed oil, 7 parts γ-aminobutyric acid, 10 parts purified water, 7 parts Ashwagandha extract, 4 parts black ginger extract, 28 parts curcumin lecithin complex, 1.8 parts grape seed extract, 0.3 parts white tomato extract, 1 part Solomon's seal extract, and 0.8 parts mulberry leaf extract.

[0020] The preparation method of the curcumin-lecithin complex is as follows: curcumin and soybean lecithin are dissolved in anhydrous ethanol at a mass ratio of 1:4, stirred in a water bath at 50°C for 1.5 h, and then the ethanol is removed by vacuum distillation. The solid is collected and dried under vacuum for 14 h to obtain the curcumin-lecithin complex.

[0021] The preparation method of the whitening composition containing astaxanthin includes the following steps: S1. Mix astaxanthin oil, grapeseed oil and curcumin lecithin, stir well to obtain the oil phase; S2, add γ-aminobutyric acid, grape seed extract, ashwagandha extract, black ginger extract, white tomato extract, Solomon's seal extract and mulberry leaf extract to purified water and stir to disperse evenly to obtain an aqueous phase; S3. Mix the aqueous phase and oil phase, stir and disperse evenly to obtain a suspension. The suspension is first treated with a high-speed dispersion homogenizer, and then treated with an ultrasonic cell disruptor. When using the high-speed dispersion homogenizer: high-speed shear dispersion at 12000r / min for 15min. When using the ultrasonic cell disruptor: ultrasonic dispersion at 100W power for 2min, followed by ultrasonic dispersion at 200W power for 4min, selecting the 1s working and 1s intermittent mode, to obtain the whitening composition. Example 4

[0022] A whitening composition capsule containing astaxanthin, the contents of which are the whitening composition of Example 1, the capsule wall containing the following components by mass fraction: gelatin 38.6%, purified water 38.6% and glycerin 22.8%.

[0023] The preparation method of the above-mentioned capsules includes the following steps: S1, after obtaining the whitening composition, vacuum degassing is performed to obtain the capsule contents; S2, in a sol tank, heat purified water to 80°C, add glycerol, mix well, and then heat to 80°C again. Turn on the vacuum pump, evacuate, and stir at 140 rpm / min. Use the feed tube to draw gelatin into the sol tank. After completion, close the feed valve and vacuum pump, and continue stirring for 20 minutes. Turn on the vacuum pump, evacuate to remove bubbles, and continue stirring at 140 rpm / min. When the temperature drops below 60°C, stop the vacuum. Heat the mixture to 62°C for later use to obtain the capsule wall material. S3, the capsule core material and capsule wall material are encapsulated in a soft capsule making machine, and the capsule wall thickness is controlled at 0.7-0.9mm. Comparative Example 1

[0024] The difference between Comparative Example 1 and Example 1 is that it does not contain Ashwagandha extract. Comparative Example 2

[0025] The difference between Comparative Example 2 and Example 1 is that it does not contain black ginger extract. Comparative Example 3

[0026] The difference between Comparative Example 3 and Example 1 is that it does not contain curcumin lecithin complex. Comparative Example 4

[0027] The difference between Comparative Example 3 and Example 1 is that it does not contain Ashwagandha extract, black ginger extract and curcumin lecithin complex. Test case

[0028] Melanin Inhibition Test The test was conducted according to the "Cosmetic Melanin Inhibition Test - Zebrafish Embryo Test Method".

[0029] The whitening compositions of Examples 1 to 3 and Comparative Examples 1 to 3 were diluted to 100 mg / L with purified water and a co-solvent (dimethyl sulfoxide) to obtain sample solutions; the total content of DMSO was 0.08 wt% of the sample solution.

[0030] Fish embryo culture medium: prepared by dissolving 2940mg anhydrous calcium chloride, 1240mg magnesium sulfate heptahydrate, 630mg sodium bicarbonate, and 55mg potassium chloride in 10L of water.

[0031] Experimental group: 24 zebrafish embryos aged 8 hours were exposed to the sample solution; Control group: 24 zebrafish embryos aged 8 hours were exposed to fish embryo culture medium; Positive control group: 24 zebrafish embryos aged 8 hours were exposed to a 30 mg / L phenylthionine aqueous solution; After exposure to a 28℃ constant temperature incubator for 48 hours, the melanin signal intensity of fish embryos was detected and statistically analyzed. Three replicates were performed for each assay. The formula for calculating the melanin inhibition rate is as follows: Melanin inhibition rate = (average signal intensity of blank control group - average signal intensity of sample solution) / (average signal intensity of blank control group - average signal intensity of positive control group) × 100%.

[0032] The statistical results are shown in Table 1 below.

[0033] Table 1. Statistical table of melanin inhibition rate of samples from Examples 1 to 3 and Comparative Examples 1 to 3. sample Melanin inhibition rate in the experimental group / % Example 1 34.35 Example 2 33.96 Example 3 35.01 Comparative Example 1 28.62 Comparative Example 2 29.88 Comparative Example 3 28.05 Comparative Example 4 26.79 As can be seen from Table 1 above, the whitening compositions of Examples 1 to 3 have a higher melanin inhibition rate compared with Comparative Examples 1 to 3.

[0034] Assay for inhibiting tyrosinase activity Tyrosinase solution was prepared using 50 mmol / L PBS buffer at pH 6.8. An 8 g / L solution of the combined composition was prepared using 40% ethanol aqueous solution. In a 96-well plate, 40 μL of 0.1 mg / mL tyrosine, 80 μL of PBS buffer, 50 μL of the combined composition solution, and 50 μL of 60 U / mL tyrosinase solution were added sequentially. After incubation at 37°C for 30 min, the enzyme activity was detected at 475 nm using a microplate reader. PBS buffer was used as a blank control. Each assay was performed in triplicate. The inhibition rate of tyrosinase activity was calculated using the following formula: Tyrosinase activity inhibition rate (%) = [(A1-A2)-(A3-A4)] / (A1-A2)x100%; In the formula, A1 is the absorbance after incubation with the blank solution, A2 is the absorbance before incubation with the blank solution, A3 is the absorbance after incubation with the composition solution, and A4 is the absorbance before incubation with the composition solution. The test results are shown in Table 2 below.

[0035] Table 2. Statistical table of inhibition rates of tyrosinase activity in samples from Examples 1 to 3 and Comparative Examples 1 to 3. sample Inhibition rate of tyrosinase activity / % Example 1 91.69 Example 2 89.44 Example 3 91.90 Comparative Example 1 77.39 Comparative Example 2 80.18 Comparative Example 3 73.59 Comparative Example 4 67.07 As can be seen from Table 2 above, the whitening composition of this application has a good inhibitory effect on tyrosinase activity.

[0036] Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that various changes can be made to it in form and detail without departing from the scope defined by the claims of the present invention.

Claims

1. A whitening composition containing astaxanthin, characterized in that: It contains the following components in parts by weight: astaxanthin oil 20.7-40.7 parts, grape seed oil 45-65 parts, γ-aminobutyric acid 5-7 parts, purified water 8-10 parts, ashwagandha extract 5-7 parts, black ginger extract 2-4 parts, curcumin-lecithin complex 20-28 parts, grape seed extract 1.4-1.8 parts, white tomato extract 0.1-0.3 parts, Solomon's seal extract 0.6-1 part, and mulberry leaf extract 0.6-0.8 parts.

2. The whitening composition containing astaxanthin according to claim 1, characterized in that: The preparation method of the curcumin-lecithin complex is as follows: curcumin and soybean lecithin are dissolved in anhydrous ethanol at a mass ratio of 1:2-4, stirred in a water bath at 50°C for 1-1.5 h, and then the ethanol is removed by vacuum distillation. The solid is collected and dried under vacuum for 12-14 h to obtain the curcumin-lecithin complex.

3. A method for preparing a whitening composition containing astaxanthin as described in any one of claims 1 or 2, characterized in that: It includes the following steps: S1. Mix astaxanthin oil, grapeseed oil and curcumin lecithin, stir well to obtain the oil phase; S2, add γ-aminobutyric acid, grape seed extract, ashwagandha extract, black ginger extract, white tomato extract, Solomon's seal extract and mulberry leaf extract to purified water and stir to disperse evenly to obtain an aqueous phase; S3. Mix the aqueous phase and oil phase, stir and disperse evenly to obtain a suspension. The suspension is first treated with a high-speed dispersion homogenizer, and then treated with an ultrasonic cell disruptor. When using the high-speed dispersion homogenizer: high-speed shear dispersion at 10000r / min-12000r / min for 9-15min. When using the ultrasonic cell disruptor: ultrasonic dispersion at 100W power for 2min, followed by ultrasonic dispersion at 200W power for 4min, selecting the 1s working and 1s intermittent mode to obtain the whitening composition.

4. A whitening composition capsule containing astaxanthin, characterized in that: The contents of the capsule are the whitening composition according to any one of claims 1-2, and the capsule wall contains the following components in mass fractions: 38.6% gelatin, 38.6% purified water and 22.8% glycerin.