Agricultural composition containing pyridaben and application thereof

Abstract

The present invention provides a pyridaben-containing pesticide composition, which comprises a first effective component and a second effective component, the first effective component is a compound having a structural formula (I), the second effective component is pyridaben, and the weight ratio of the first effective component to the second effective component is 1-50: 1-50. The invention provides an application of the pesticide composition in prevention and control of agricultural and forestry pests, especially in prevention and control of phyllotreta striolata. The invention verifies that the binary composition obtained by compounding the flufenoxanil and the pyridaben in a proper proportion has a remarkable synergistic effect, so that the effects of improving the control effect, reducing the dosage and expanding the insecticidal spectrum are achieved. The composition disclosed by the invention has an excellent control effect on target pests such as chilo suppressalis, plutella xylostella, green peach aphid, beet armyworm, ostrinia nubilalis, thrips, flea beetle, spider mites, brown planthopper or liriomyza sativae. (I)

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticides. More particularly, the present application relates to a pyridaben-containing agricultural composition, and to the use of the agricultural composition. BACKGROUND

[0002] In the field of agriculture and forestry, crop damage caused by insect pests has always been a serious challenge. Currently, agricultural pests have developed resistance to traditional chemical insecticides, and most existing pesticides also have environmental risks, so there is an urgent need to develop new insecticides with higher biological activity, lower application dosage, and better environmental compatibility. At present, scientific research institutions and enterprises at home and abroad have successfully developed a variety of new insecticides with high efficiency, low dosage, and environmental friendliness. Under this background, the efficient development of new insecticide compound and mixed formulations has also been highly valued. Scientific and reasonable compound or mixed formulation scheme can not only effectively broaden the control spectrum, improve the control efficiency, reduce the pesticide dosage, and reduce the risk of pesticide damage, but also delay the development of target biological resistance.

[0003] Broflanilide (English name: Broflanilide, CAS No. 1207727-04-5) is a benzene-based benzamide insecticide jointly developed by Japan's Mitsui Chemicals and BASF, mainly used for preventing and controlling common pests such as Lepidoptera, Coleoptera, termites, and mosquitoes and flies on crops.

[0004] In order to further improve the activity of benzene-based benzamide insecticides, Chinese invention patent CN115925576B discloses a double amide compound containing heptafluoroisopropyl and a preparation method thereof. The compound has the structure shown in general formula (II):

[0005]

[0006] (II)

[0007] According to the description in its specification, SYN003 (Chinese generic name: flubrocydamide) with chemical formula I has excellent high insecticidal activity against resistant populations at low doses, has insecticidal activity against pests including Lepidoptera, Coleoptera, Hemiptera, Thysanoptera, Diptera, Orthoptera, Homoptera, Isoptera, Hymenoptera, spider mites, nematodes, mosquitoes, flies, ants, etc., has good speed, simple preparation method, high efficiency, easy industrialization production, and has broad application prospects in the fields of agriculture, forestry, and horticulture for controlling pests.

[0008]

[0009] (I)

[0010] The above-mentioned compound of formula (I) has better insecticidal activity on Chilo suppressalis, Plutella xylostella and Spodoptera frugiperda than bromofenprox, especially in low dose and rapid onset. However, although the compound of formula (I) is widely concerned due to its novel action target, its resistance risk increases significantly with the extension of use time. Therefore, it is still desirable to develop more efficient insecticidal compositions to meet the needs of the agricultural and forestry fields.

[0011] On the other hand, there is currently no relevant research report on the compound I and pyridaben complex composition, which provides a new breakthrough point for the field of agricultural compositions. SUMMARY

[0012] The purpose of the present application is to overcome the defects of the prior art and provide a new agricultural composition containing pyridaben.

[0013] The idea of the present application is based on further research on bromofenprox and the above-mentioned compound of formula (I), screening appropriate drug combination schemes, and verifying the synergistic effect of the drug composition containing the compound of formula (I) through experiments.

[0014] Although the prior art has certain research on drug compositions containing bromofenprox, however, the introduction of alkoxy group usually has a significant effect on the activity of the compound. Design, Synthesis, and Insecticidal Activities of Novel N-Alkoxyalkyl Meta-Diamides as Potential GABAR Antagonists, Sha Zhou et al. Journal of Agricultural and Food Chemistry 2025 73 (28), 17446-17457 studied the effect of introducing alkyl or ether bond at R5 position on the activity of Plutella xylostella, and the results showed that introducing alkyl (compound le in Table 2 in the literature) or alkoxy (compounds lld or lle) had a significant effect on resistance, wherein compound le had insufficient activity on Plutella xylostella to meet the requirements of insecticides, and compounds lld and lle showed excellent performance. As can be seen, although the compounds recorded in Table 2 in the literature all have the same parent nucleus structure, when different groups are introduced at R5 position, the final insecticidal activity cannot be expected.

[0015] In order to further study the activity difference between bromofenprox and the compound of formula (I), the inventors commissioned a third party to conduct bioassay experiments of these two compounds on Phyllotreta striolata in Guangdong Province in 2024, and the test crop was Mingyuanjianye 50-day-old beet heart, and the results were as follows:

[0016]

[0017] The inventors commissioned a third party to conduct bioassay experiments of the two compounds on C. medinalis in Jiangsu Province in 2024 according to the Guidelines for Field Efficacy Trials - Insecticides for Controlling C. medinalis, and the test crop was rice Wei Liangyou 8612, and the control effects were as follows:

[0018] Formulation Rate Rep. 1 Rep. 2 Rep. 3 Total Eff. 5% Diff. Compound of Formula (I) SC 10 ml / acre 4 3 0 7 85.93 Bcc Compound of Formula (I) SC 15 ml / acre 3 0 1 4 90.24 Cc Compound of Formula (I) EC 10 ml / acre 2 0 2 4 90.24 Cc Bistrifluron SC 30 ml / acre 8 6 6 20 51.22 Bb Untreated check / 13 19 9 41 0 Aa

[0019] The inventors commissioned a third party to conduct bioassay experiments of the two compounds on C. medinalis in Jiangsu Province in 2024 according to the Guidelines for Field Efficacy Trials - Insecticides for Controlling C. medinalis, and the test crop was rice Wei Liangyou 8612, and the control effects were as follows:

[0020] Formulation Rate Rep. 1 Rep. 2 Rep. 3 Total Eff. 5% Diff. Compound of Formula (I) SC 6 ml / acre 53 46 34 133 86.01 d Compound of Formula (I) SC 10 ml / acre 33 27 40 100 89.48 e Bistrifluron SC 15 ml / acre 207 244 206 657 30.91 b Untreated check / 297 343 311 951 0.00 a

[0021] The above experimental results show that although bromofluorfenisulam and the compound of formula (I) have the same parent nucleus structure and have certain similarity, the inventors have confirmed through early research that there is a significant difference in their insecticidal activity, and this difference is difficult to predict and can only be confirmed through experiments.

[0022] In view of this, the present application provides an agricultural composition, which is composed of a first active ingredient and a second active ingredient, the first active ingredient is fluoro-bromfenisulam (compound of formula (I)), and the second active ingredient is pyridaben.

[0023] In the present application, the flubrocydamide is 2-fluoro-3-{[(4-fluorophenyl)carbonyl](2-methoxyethyl)amino}-N-[2-bromo-4-(1,1,1,2,3,3,3-heptafluoropropan-2-yl)-6-(trifluoromethyl)phenyl]benzamide (also written as N-[2-bromo-4-(1,1,1,2,3,3,3-heptafluoropropan-2-yl)-6-(trifluoromethyl)phenyl]-2-fluoro-3-[4-fluoro-N-(2-methoxyethyl)benzamido]benzamide in some prior art) prepared according to the method described in the specification of Chinese Invention Patent CN 115925576B. According to the description of its specification, according to the indoor biological activity determination of Plutella xylostella, the insecticidal rate of the compound reaches 60% for the resistant population of Plutella xylostella and 70% for the sensitive population at a concentration of 0.05 mg / L, and the insecticidal rate of the compound reaches 100% for both the resistant population and the sensitive population of Plutella xylostella at a concentration of 0.1-0.2 mg / L. According to the indoor biological activity determination of Chilo suppressalis, the insecticidal rate of the compound reaches 83.6% for Chilo suppressalis at a concentration of 0.125 mg / L, and the insecticidal rate of the compound reaches 100% for Chilo suppressalis at a concentration of 0.25 mg / L or above. According to the indoor biological activity determination of Spodoptera exigua, the insecticidal rate of the compound reaches 100% for Spodoptera exigua at a concentration of 0.125 mg / L or above, showing good insecticidal activity.

[0024] The second active ingredient is pyridaben (chemical name 2-tert-butyl-5-(4-tert-butylbenzylthio)-4-chloropyridazin-3(2H)-one), which is a highly effective and broad-spectrum miticide belonging to the pyridazine class of insecticides and miticides. It is mainly used to control various types of mite pests on crops such as fruit trees, vegetables, and cotton, and has excellent control effects on red spider mites (such as two-spotted spider mites, carmine spider mites, and hawthorn spider mites) and rust mites. Pyridaben exerts its effects through contact and stomach poisoning, and can quickly inhibit the activity and reproduction of mites after application, thereby effectively controlling the spread of mite damage. It is obtained from Xinyi Taisheng Chemical Co., Ltd.

[0025] In the present application, the weight ratio of the first active ingredient to the second active ingredient is 1-50:1-50.

[0026] Based on this, the present application also provides an agricultural insecticide containing 0.5-95.0% by weight of the above-mentioned agricultural composition and the balance being an agriculturally acceptable adjuvant.

[0027] The dosage form of the agricultural composition of the present application is soluble liquid, soluble powder, soluble granule, emulsifiable concentrate, wettable powder, aqueous emulsion, suspension, dispersible oil suspension, water dispersible granule, microcapsule suspension, granule, microemulsion, suspended emulsion, microcapsule suspension-suspension, ultra-low volume liquid, hot fogging, spreader-sticker, suspension seed coating, seed treatment dry powder, seed treatment suspension, seed treatment soluble powder, seed treatment dispersible powder, seed treatment emulsion, or seed treatment liquid. The use mode of the pharmaceutical composition or pharmaceutical preparation is spraying, soil treatment, or seed treatment, etc.

[0028] Preferably, the dosage form of the agricultural composition is soluble liquid, soluble granule, suspension, emulsifiable concentrate, wettable powder, aqueous emulsion, water dispersible granule, dispersible oil suspension, microcapsule suspension, ultra-low volume liquid, hot fogging, suspension seed coating, or seed treatment dispersible powder.

[0029] The carrier is a solid carrier or a liquid carrier. The solid carrier is selected from silicate, carbonate, sulfate, oxide, alkali metal salt, or vegetable carrier. The liquid carrier is selected from water, aromatic hydrocarbon, chlorinated aliphatic hydrocarbon, aliphatic hydrocarbon, alcohol, ether, ketone, vegetable oil, or methylated vegetable oil.

[0030] The adjuvant is selected from wetting agent, dispersant, emulsifier, surfactant, antifoaming agent, thickening agent, preservative, and antifreeze. The wetting agent is polyoxyethylene alkyl aryl ether, polyoxyethylene alkyl ether, alkyl benzene sulfonate, or alkyl naphthalene sulfonate, etc. The dispersant is sodium sulfate, sodium chloride, alkyl naphthalene sulfonate, alkyl phenol polyoxyethylene ether, etc. The emulsifier is polyoxyethylene ester and ether, sodium dodecyl benzene sulfonate, etc. The surfactant is the surfactant used in emulsifier, dispersant, stabilizer, or wetting agent. The antifoaming agent is selected from Antifoam, silicone, C8-10 fatty alcohol, C10-20 saturated fatty acid, or amide antifoaming agent. The thickening agent is selected from carboxymethyl alcohol, polyvinyl alcohol, polyvinyl acetate, xanthan gum, gelatin, gum arabic, polyvinyl pyrrolidone, magnesium aluminum silicate, polyethylene glycol, phenol formaldehyde resin, shellac, carboxymethyl cellulose, or sodium alginate. The preservative is selected from sodium benzoate, carboxin, potassium sorbate, p-hydroxy benzoic acid ester, etc. The antifreeze is selected from glycerol, urea, ethylene glycol, or propylene glycol.

[0031] The preparation methods of these dosage forms are all conventional, for example, the preparation method of the wettable powder is as follows: 0.5% to 80% by weight of the agricultural insecticidal composition, 2% to 10% of a wetting agent, 5% to 10% of a dispersing agent, and the balance to 100% of a filler are thoroughly mixed, and then a superfine pulverizer or an air flow pulverizer commonly used in the art is used for pulverization to obtain the wettable powder. The filler used in the present application is kaolin, diatomite, mica powder, bentonite, attapulgite, or light calcium carbonate; other raw materials used in the present application are as described above, and thus are not repeated here.

[0032] The preparation method of the suspension concentrate is as follows: 0.5% to 50% by weight of the agricultural insecticidal composition, 1% to 5% of a wetting agent, 1% to 8% of a dispersing agent, 0.1% to 2% of a thickening agent, 2% to 5% of an antifreezing agent, and 0.2% to 1% of an antifoaming agent are mixed with water to make up 100%, and then sand milling is performed in a sand mill to a particle size of 2 to 5 microns, and then the thickening agent is added to a preparation kettle and shearing stirring is performed to obtain the suspension concentrate.

[0033] The preparation method of the microemulsion is as follows: 0.5% to 40% by weight of the agricultural insecticidal composition, 1% to 30% of an emulsifier, and 0% to 50% of a solvent are added to a preparation kettle, stirring is performed until complete dissolution, and then stirring is continuously performed until uniform mixing to obtain the microemulsion. The solvent used in the preparation of the microemulsion in the present application is, for example, isopropyl alcohol or cyclohexanone, and thus is not repeated here. They are all products currently sold on the market, for example, isopropyl alcohol products sold by Shandong Jinan Guota Chemical Co., Ltd. or cyclohexanone products sold by Nanjing Desman Company.

[0034] The preparation method of the emulsion in water is as follows: 0.5% to 40% by weight of the agricultural insecticidal composition, 1% to 20% of an emulsifier, 1% to 15% of a cosolvent, and 10% to 40% of a solvent are added to a preparation kettle, stirring and dissolution are performed, and then the mixture is added to a high-shear stirring kettle in which water has been weighed to make up 100%, and then high-shear stirring is continuously performed until uniform mixing to obtain the emulsion in water.

[0035] The preparation method of the suspension-emulsion is as follows: taking 0.5%-40% by weight of the agricultural insecticidal composition, 1%-5% of a solvent, 1%-5% of a dispersant, 1%-10% of an emulsifier, 1%-5% of a wetting agent, 1%-5% of an antifreezing agent, 0.1%-1% of a thickening agent, 0.1%-1% of an antifoaming agent, and water to 100%, then preparing the fluoroimid into an emulsion in water according to the preparation method of the emulsion in water above, and preparing the pyridaben into a suspension according to the preparation method of the suspension above, then stirring and mixing the emulsion in water and the suspension uniformly to obtain the suspension-emulsion. Alternatively, the pyridaben is prepared into an emulsion in water according to the preparation method of the emulsion in water above, and the fluoroimid is prepared into a suspension according to the preparation method of the suspension above, then stirring and mixing the emulsion in water and the suspension uniformly to obtain the suspension-emulsion.

[0036] The preparation method of the water dispersible granule is as follows: taking 0.5%-70% by weight of the agricultural insecticidal composition, 1%-15% of a wetting agent, 1%-15% of a dispersant, 1%-10% of a disintegrant, and a filler to 100%, mixing and crushing them by an airflow pulverizer, then adding water to knead, granulating in a granulator, drying, and screening to obtain the water dispersible granule. The disintegrant used in the present application is ammonium sulfate or sodium carboxymethyl cellulose; and the filler used in the present application is kaolin, diatomite, or corn starch.

[0037] The preparation method of the microcapsule suspension-suspension is as follows: taking 0.5%-40% by weight of the agricultural insecticidal composition, 1%-20% of an emulsifier, 1%-20% of a solvent, 1%-10% of a dispersant, 1%-5% of a wetting agent, 3%-5% of a capsule skin material, 1%-2% of an initiator, 1%-5% of a pH regulator, 0.1%-1% of a thickening agent, 1%-5% of an antifreezing agent, 0.1%-1% of an antifoaming agent, 0.1%-1% of a preservative, and water to 100%. Taking some active components, dissolving the raw drug in the solvent, adding the emulsifier and the capsule skin material, mixing uniformly to prepare an oil phase, adding the dispersant and water in another preparation kettle to prepare an aqueous phase, dispersing the oil phase in the aqueous phase to make it into an emulsion in water, adding the initiator to perform a capsule reaction, then keeping warm at about 50°C for 2-3 hours to make the capsule skin solidify, adjusting the pH value, and reserving the semi-finished product. Another component is sheared and milled by adding the dispersant, the wetting agent, the antifreezing agent, and other auxiliary agents according to the preparation method of the suspension, and then the material is discharged after reaching the quality standard of the suspension; the microcapsule suspension-suspension is obtained by mixing the microcapsule suspension and the suspension uniformly according to the proportion. The capsule skin material used in the present application is a modified isocyanate.

[0038] The preparation method of the emulsifiable concentrate is as follows: 0.5% to 40% of the agricultural insecticidal composition, 1% to 30% of an emulsifier, 0% to 20% of a cosolvent and a solvent supplemented to 100% are added into a preparation kettle, stirred uniformly and dissolved completely, stirred and mixed uniformly to obtain the emulsifiable concentrate. The cosolvent used in the preparation of the emulsifiable concentrate of the present application is, for example, isopropyl alcohol, cyclohexanone, N-methyl pyrrolidone and the like; the same applies to the following, and thus will not be described again.

[0039] The preparation process and the selection of the adjuvant and excipient of these dosage forms all belong to the prior art in the field and are the knowledge mastered by the skilled in the art, and thus will not be described again.

[0040] In the present application, the agricultural composition is used for preparing an agricultural drug for preventing and controlling pests on fruit trees, vegetables, ornamental plants, tea, cotton, cereal crops, shrubs, broad-leaved trees, evergreen trees and urban health pests. The composition can also be used for preparing a drug for preventing or controlling pests including pests of Lepidoptera, Coleoptera, Hemiptera, Thysanoptera, Diptera, Orthoptera, Homoptera, Isoptera, Hymenoptera, spider mites and nematodes, mosquitoes, flies, ants and their larvae and eggs. The pests include, but are not limited to, cotton bollworm, diamondback moth, beet armyworm, oriental armyworm, cabbage worm, yellow borer, rice borer, rice leaf roller, rice plant hopper, western flower thrips, melon thrips, onion thrips, ginger thrips, mango thrips, peach aphid, cotton aphid, alfalfa aphid, apple yellow aphid, wheat aphid, flea beetle, stink bug, brown planthopper, white-backed planthopper, termite, mosquito, red spider mite and / or citrus red spider mite.

[0041] The plants to which the agricultural composition of the present application can be applied mainly include the following categories: vegetables such as cucumber, sponge gourd, watermelon, melon, pumpkin, bottle gourd, spinach, celery, cabbage, Chinese cabbage, gourd, pepper, eggplant, tomato, onion, ginger, garlic, leek, lettuce, bean, rainbow bean, broad bean, radish, carrot, potato, yam; cereal crops such as wheat, barley, corn, rice, sorghum; fruit trees such as apple, pear, banana, citrus, grape, litchi, mango; flowers such as peony, Chinese rose, fire crane; oil crops such as peanut, soybean, rape, sunflower, sesame; sugar crops such as sugar beet, sugarcane; and other crops such as strawberry, potato, sweet potato, tobacco and tea. The agricultural composition of the present application can also be applied in the fields of gardening, forestry, household health and / or public health and the like.

[0042] The present application is proved by experiments that the binary composition obtained by compounding flubrocydamide and pyridaben at a suitable ratio has a significant synergistic effect, thereby achieving the effects of improving the control effect, reducing the amount of drug and expanding the insecticidal spectrum. The composition of the present application has excellent control effect on target pests such as diamondback moth, cabbage worm, peach aphid, beet armyworm, corn borer, thrips, flea beetle, spider mite, brown planthopper or american planthopper. DETAILED DESCRIPTION

[0043] The following examples are intended to illustrate the present application but not limit its scope.

[0044] In the present application, unless otherwise specified, "%" used to explain concentration is all weight percent, and ":" is all weight ratio.

[0045] Example 1: Preparation of 38% flubrocydamide·pyridaben wettable powder

[0046] Take 28% of chemical structural formula (I) flubrocydamide, 10% pyridaben, 4% sodium dodecyl sulfate, 8% aryl sulfonic acid sodium salt, 4% ammonium sulfate, 10% kaolin, and supplement to 100% talc, based on the total weight of the present insecticide wettable powder, to obtain the present insecticide wettable powder according to the preparation method described in the specification.

[0047] Example 2: Preparation of 30% flubrocydamide·pyridaben water dispersible granule

[0048] Take 20% of chemical structural formula (I) flubrocydamide, 10% pyridaben, 4% sulfosuccinyl sodium salt, 8% sodium lignosulfonate, 4% polyvinylpyrrolidone, 20% kaolin, and supplement to 100% corn starch, based on the total weight of the present insecticide water dispersible granule, to obtain the present insecticide water dispersible granule according to the preparation method described in the specification.

[0049] Example 3: Preparation of 25% flubrocydamide·pyridaben suspension concentrate

[0050] Take 15% of chemical structural formula (I) flubrocydamide, 10% pyridaben, 2% polyoxyethylene sorbitan fatty acid ester, 2% modified styrene maleic acid copolymer, 2% EO-PO fatty alcohol polyether, 0.2% xanthan gum, 1.8% magnesium aluminum silicate, 1% white carbon black, 0.2% sodium benzoate, 4% ethylene glycol, and supplement to 100% water, based on the total weight of the present insecticide suspension concentrate, to obtain the present insecticide suspension concentrate according to the preparation method described in the specification.

[0051] Example 4: Preparation of 20% flubrocydamide·pyridaben emulsifiable concentrate

[0052] Take 10% of chemical structural formula (I) flubrocydamide, 10% pyridaben, 4% fatty alcohol polyoxyethylene ether, 5% castor oil polyoxyethylene ether, 5% calcium dodecylbenzenesulfonate, 3% triphenylethyl phenol polyoxyethylene ether sulfate, 20% cyclohexanone, 5% N-methyl pyrrolidone, and supplement to 100% S-200 aromatic hydrocarbon solvent, based on the total weight of the present insecticide emulsifiable concentrate, to obtain the present insecticide emulsifiable concentrate according to the preparation method described in the specification.

[0053] Example 5: Preparation of 18% flubrocydamide·pyridaben water emulsion

[0054] Take 8% of the chemical structure of formula (I) of flubrocythrinate, 10% of pyridaben, 6% of cyclohexanone, 2% of dimethylformamide, 20% of aromatic hydrocarbon solvent, 6% of sodium dodecylbenzenesulfonate, 5% of fatty alcohol polyoxyethylene ether, 2% of castor oil polyoxyethylene ether, 0.3% of defoaming agent, 5% of propylene glycol and supplement to 100% of deionized water, according to the preparation method described in the specification of the application, the insecticide emulsion in water of the application is obtained.

[0055] Example 6: Preparation of 10% flubrocythrinate·pyridaben microemulsion

[0056] Take 5% of the chemical structure of formula (I) of flubrocythrinate, 5% of pyridaben, 20% of dimethyl sulfoxide, 6% of N-methyl pyrrolidone, 4% of n-butanol, 6% of triphenyl ethyl phenol polyoxyethylene ether, 7% of dodecylbenzenesulfonate and supplement to 100% of water, according to the preparation method described in the specification of the application, the insecticide microemulsion of the application is obtained.

[0057] Example 7: Preparation of 18% flubrocythrinate·pyridaben suspoemulsion

[0058] Take 10% of the chemical structure of formula (I) of flubrocythrinate, 6% of phosphate and sulfonate mixture, 2% of EO-PO block polyether, 2% of styryl phenol polyoxyethylene ether phosphate, 1% of magnesium aluminum silicate, 1% of white carbon black, 0.2% of xanthan gum, 5% of ethylene glycol, 0.2% of kathon, 0.4% of defoaming agent and appropriate amount of deionized water; 8% of pyridaben, 6% of N-methyl pyrrolidone, 8% of cyclohexanone, 3% of carboxylic acid ester, 2% of EO-PO block copolymer, 5% of propylene glycol, 0.2% of defoaming agent and appropriate amount of deionized water, according to the preparation method described in the specification of the application, the insecticide suspoemulsion of the application is obtained.

[0059] Example 8: Preparation of 10% flubrocythrinate·pyridaben microcapsule suspension-suspension agent

[0060] Take 10% of the chemical structure of formula (I) of flubrocythrinate, 2% of dimethylformamide, 8% of cyclohexanone, 2% of carboxylic acid ester, 2% of non-ionic polyether sulfonate, 4% of modified isocyanate, 1% of initiator, 1% of pH regulator and supplement to 100% of deionized water, according to the preparation method described in the specification of the application, the microcapsule suspension agent is obtained; 10% of pyridaben, 4% of polycarboxylate, 2% of sodium lignosulfonate, 2% of fatty acid derivative, 0.2% of xanthan gum, 1% of magnesium aluminum silicate, 1% of white carbon black, 0.2% of sodium benzoate, 0.4% of silicone defoaming agent, 5% of propylene glycol and supplement to 100% of deionized water, according to the preparation method described in the specification of the application, the suspension agent is obtained, and the two are mixed in proportion to obtain the microcapsule suspension-suspension agent.

[0061] Bioassay Example 1 Indoor combined toxicity determination test of Phyllotreta striolata

[0062] Test target: Phyllotreta striolata.

[0063] Test method: A series of drug solutions with certain concentration gradient were prepared, clean cabbage leaves were immersed in the drug solution for 10s, then taken out, naturally dried, and placed in a 9cm diameter culture dish with filter paper for moisture, 20 CO2 anesthetized P. striolata adults were added, immediately covered with a culture dish cover, and the culture dish was stabilized with a rubber band to prevent the P. striolata from escaping. Each treatment was repeated 3 times, and distilled water containing organic solvent was used as a blank control. The treated test materials were placed in an observation room, and the temperature, humidity and light in the observation room were adjusted as needed. After 48 hours, the number of dead and live insects was investigated, and the mortality of each pesticide treatment was calculated. The co-toxicity coefficient of each ratio was calculated by Suny-p method in bioassay example 1, and the mixing effect was evaluated. The test results are shown in Table 1.

[0064] The co-toxicity coefficient (CTC value) of the mixed agent was calculated according to the following formula:

[0065]

[0066] In the formula: ATI-observed toxicity index of mixed agent; S-LC 50 of standard pesticide; M-LC 50 of mixed agent.

[0067]

[0068] In the formula: TTI-theoretical toxicity index of mixed agent; TIA-toxicity index of pesticide A; PA-percent content of pesticide A in mixed agent; TIB-toxicity index of pesticide B; PB-percent content of pesticide B in mixed agent.

[0069]

[0070] In the formula: CTC-co-toxicity coefficient; ATI-observed toxicity index of mixed agent; TTI-theoretical toxicity index of mixed agent.

[0071] When the co-toxicity coefficient is greater than 120, it indicates that the mixed preparation has a synergistic effect; when the co-toxicity coefficient is less than 80, it indicates that the mixed preparation has an antagonistic effect; and when the co-toxicity coefficient is greater than 80 but less than 120, it indicates that the mixed preparation has an additive effect.

[0072] The results of indoor combined toxicity determination of flubrocydamide and pyridaben on P. striolata are shown in Table 1.

[0073] Table 1

[0074] Formulation LC 50 (mg / L) ATI TTI Co-toxicity factor CTC Flubrocythrinate (A) 0.0448 10174.3304 - - Pyridaben (B) 4.5581 100.0000 - - A:B (1:50) 1.1999 379.8733 297.5359 127.6731 A:B (1:20) 0.6248 729.5294 579.7300 125.8395 A:B (1:10) 0.3325 1370.8571 1015.8482 134.9470 A:B (1:5) 0.1807 2522.4682 1779.0551 141.7870 A:B (1:1) 0.0631 7223.6133 5137.1652 140.6148 A:B (5:1) 0.0353 12912.4646 8495.2753 151.9958 A:B (10:1) 0.0305 14944.5902 9258.4821 161.4151 A:B (20:1) 0.0378 12058.4656 9694.6003 124.3833 A:B (50:1) 0.0362 12591.4365 9976.7945 126.2072

[0075] From the above indoor virulence determination results, it can be seen that the mixture of flubrocydamid and pyridaben shows synergistic effect on Phyllotreta striolata in the range of 1:50 to 50:1 in weight ratio. Among them, the synergistic effect is more obvious in the range of 1:5 to 10:1 in weight ratio. The results show that the mixture of flubrocydamid and pyridaben has rationality and feasibility for controlling Phyllotreta striolata.

Claims

1. A pesticide composition containing pyridaben, characterized in that... The pesticide composition comprises a first active ingredient and a second active ingredient, wherein the first active ingredient is a compound having the following structural formula (I): （I） The second active ingredient is pyridaben.

2. The pesticide composition according to claim 1, characterized in that... The weight ratio of the first active ingredient to the second active ingredient is 1~50:1~50.

3. An agricultural insecticide, characterized in that... It contains 0.5 to 95.0% by weight of the pesticide composition as described in claim 1 or 2 and the balance being an agriculturally acceptable adjuvant.

4. The agricultural insecticide according to claim 3, characterized in that... The insecticide is formulated as a wettable powder, suspension concentrate, suspension emulsion, microemulsion, water emulsion, water-dispersible granules, emulsifiable concentrate, or microcapsule suspension.

5. The application of the pesticide composition according to claim 1 or 2 or the agricultural insecticide according to claim 3 in the control of agricultural and forestry pests.

6. The application according to claim 5, characterized in that... The agricultural and forestry pest mentioned is the yellow-striped flea beetle.

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