Composition containing trehalose-6-phosphoric acid and application of composition in prevention and control of high-temperature heat damage to crops

Abstract

The invention provides a composition containing trehalose-6-phosphoric acid and application of the composition in prevention and control of high-temperature heat damage to crops, based on 100 parts by weight of the total weight of the composition, the composition comprises 0.25 parts by weight or more of trehalose-6-phosphoric acid, 0.5 parts by weight or more of Ectoine and 2.6 parts by weight or more of chelated calcium, the weight of the chelated calcium is based on the weight of calcium contained in the chelated calcium. The composition can reduce yellowing and withered leaves of crops and reduce the blighted grain rate, has environmental friendliness and application convenience, and has wide application prospects in prevention and control of high-temperature heat damage of the crops.

Description

Technical Field

[0001] This invention relates to the field of preventing and controlling high-temperature heat damage to crops, specifically to a composition containing trehalose-6-phosphate and its application in preventing and controlling high-temperature heat damage to crops. Background Technology

[0002] Rice is one of the world's most important food crops and a crucial cereal crop, with its yield and quality directly impacting food security. However, with the intensification of global warming, high-temperature heat stress has become a key abiotic stress factor limiting high and stable rice yields. High-temperature heat stress generally refers to an agricultural meteorological disaster caused by temperatures exceeding the upper limit of rice growth temperature during the rice's booting, heading, flowering, or grain-filling stages. This affects normal leaf photosynthesis, pollen development, flowering, grain filling, and ripening, resulting in reduced grain number per panicle and thousand-grain weight, increased empty grain rate, and consequently, decreased yield and deteriorated quality. Studies have shown that rice is extremely sensitive to temperature; for every 1°C increase in global average temperature, rice yield decreases by an average of 3.2%. Especially during the heading and flowering stage to the grain-filling stage, when the ambient temperature consistently exceeds the suitable growth threshold for rice (daily maximum temperature ≥35℃ or daily average temperature ≥30℃), it can trigger "high-temperature heat injury," leading to a series of physiological dysfunctions, including: High-temperature stress accelerates leaf senescence and reduces carbohydrate synthesis: High temperatures generate large amounts of reactive oxygen species (ROS), exacerbating oxidative damage to cell membranes, causing yellowing and wilting of leaves, premature senescence, and hindered carbohydrate synthesis. Grain development is impaired, resulting in an increase in empty grains and a decrease in yield: High temperatures during the grain-filling stage reduce the synthesis and translocation capacity of photosynthetic products (such as sucrose and starch), leading to insufficient grain filling and a decrease in thousand-grain weight.

[0003] Currently, the main measures to address high-temperature heat damage to rice in agricultural production include: breeding heat-resistant varieties (long cycle, limited adaptability), irrigation cooling (high water consumption, unstable effect), and foliar spraying of chemical regulators (such as salicylic acid and abscisic acid, but with poor effect).

[0004] Therefore, there is an urgent need for a compound fertilizer that can solve the problem of high-temperature heat damage to crops and increase crop yield. Summary of the Invention

[0005] Technical Purpose The technical objective of this invention is to provide a composition that solves the technical problem of crop yield reduction due to high-temperature heat damage. Attached Figure Description

[0006] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0007] Figure 1 This is a comparison chart of MDA content in heat-damaged rice after 48 hours of treatment with different concentrations of ectoine in Example 1 of the present invention.

[0008] Figure 2 This is a comparison chart of MDA content in heat-damaged rice after 48 hours of treatment with different concentrations of chelated calcium in Example 1 of the present invention.

[0009] Figure 3 This is a comparison chart of MDA content in heat-damaged rice after 48 hours of treatment with a mixture of ectoine and sugar alcohol calcium in Example 1 of the present invention.

[0010] Figure 4 This is a comparison chart of the heat damage index of rice caused by the combination of ectoine and sugar alcohol calcium in Example 1 of the present invention. Detailed Implementation

[0011] The technical solutions of this application are described in detail below through specific embodiments to enable those skilled in the art to better understand this application. However, the provision of these embodiments is not intended to limit the scope of protection of this application.

[0012] the term High temperature heat damage: refers to environmental conditions in which the daily maximum temperature is ≥35℃ or the daily average temperature is ≥30℃ for more than 3 consecutive days from the heading and flowering stage to the grain filling stage of rice.

[0013] Heat damage grading standards: According to Sun Shengnan's method, heat damage is divided into 5 levels: Level 0: No heat damage symptoms; Level 1: Cotyledons are slightly yellow, 1-2 leaves have yellow spots, leaf margins are slightly yellow, and the margins of the heart leaves are slightly yellow; Level 2: Cotyledons turn yellow, small areas of the leaves have yellow spots, and the heart leaves are yellowed and dried up; Level 3: Cotyledon margins are necrotic and dried up, most leaves are yellowed, margins are necrotic and dried up, and most of the heart leaves are necrotic and yellowed; Level 4: The entire plant's leaves wilt, die, and dry up.

[0014] T6P (trehalose-6-phosphate): Chemical formula C 12 H 23 O 15 P, with a molecular weight of 410.28, is a key precursor for trehalose synthesis and also an important signaling molecule regulating carbon allocation in plants.

[0015] Technical principles of this application Under high-temperature stress, rice leaves experience excessive transpiration and water loss, and the accumulation of peroxides causes cell membrane damage, leading to leaf wilting and yellowing, and weakened photosynthesis at the rice source end. Simultaneously, the expression of sucrose transport proteins in the grains is suppressed, and the activity of starch synthase is reduced, resulting in the ineffective transport of photosynthetic products. The compound composition of this invention addresses the above-mentioned technical problems through the following mechanism: T6P activates the SnRK1 / SnRK2 signaling pathway in rice grains, upregulates the expression of sucrose transporter genes (such as OsSUT1) and starch synthase genes (such as OsSSIIa), promotes the transport of sucrose synthesized in leaves to grains under high temperature stress, accelerates starch accumulation, and reduces the formation of empty grains. Ectocin contains substances that scavenge reactive oxygen species, maintain cell osmotic pressure balance to reduce water loss, and protect cell membranes. It helps prevent damage to cell membranes caused by excessive accumulation of reactive oxygen species under high-temperature stress, as well as cell death due to transpiration and water loss at high temperatures, leading to leaf drying and an increase in empty grains.

[0016] Sugar alcohol calcium acts as a signaling molecule, increasing the activity of core antioxidant enzymes such as superoxide dismutase (SOD) and catalase (CAT), thereby enhancing crops' ability to scavenge reactive oxygen species.

[0017] Experimental and testing methods: Indoor simulation of high-temperature heat damage to rice. This technical solution explored indoor bioassay simulation conditions for high-temperature heat damage in rice. In an artificial climate chamber, rice was subjected to different daytime temperatures of 30℃, 35℃, and 40℃, with a fixed nighttime temperature of 25℃, for 12 hours each day and night. MDA levels in leaves were measured at 0, 6, 12, 18, 24, 48, and 72 hours. The results showed that the treatment with 40℃ during the day and 25℃ at night exhibited more pronounced and regular changes in malondialdehyde (MDA) levels in the leaves, making it suitable for simulating environmental conditions in experiments.

[0018] In the following embodiments, the application method for each experimental group was to spray each experimental group with foliar spray after rice stress, spraying the front and back of the leaves evenly once. It should be noted that the spray treatment amount of each experimental group can be regarded as the same, that is, the total application amount of different concentration treatment groups is the same. For example, when applying the combination of T6P concentration of 20 mg / L + Ectoin concentration of 20 mg / L + Sugar alcohol calcium concentration of 62.5 mg / L (that is, the weight fractions of T6P, Ectoin, and Sugar alcohol calcium are 4%, 4%, and 5.2%, respectively), the weight parts of T6P, Ectoin, and Sugar alcohol calcium in this combination are 4 parts by weight of T6P, 4 parts by weight of Ectoin, and 5.2 parts by weight of Sugar alcohol calcium.

[0019] Determination of malondialdehyde (MDA) content.

[0020] The content of malondialdehyde (MDA) was determined by the thiobarbituric acid (TBA) colorimetric method.

[0021] Empty grain rate survey: For each experimental plot, five points were sampled along the diagonal. One square meter of rice panicles were collected at each sampling point. The number of empty and chaff grains and the total number of grains were counted. The empty and chaff rate was calculated as (number of empty and chaff grains * 100 / total number of grains).

[0022] Experimental materials: T6P (trehalose-6-phosphate): A product of SIGMA, with a purity of 95%. Ectocin (tetrahydropyrimidine): A product of SIGMA, with a purity of 95%; Sorbitol calcium: Guangdong Mingtong Biotechnology Co., Ltd., content 98%; Salicylic acid: a product of SIGMA, with a purity of 99%; Calcium nitrate: SIGMA product, 99.9% purity; Calcium chloride: a product of SIGMA, with a purity of 99.9%; Trehalose: A product of SIGMA, with a purity of 99%; EDTA-Calcium: SIGMA product, 98% purity.

[0023] Note: The calcium concentrations mentioned in the following examples refer to the concentration of calcium element in the corresponding calcium reagents mentioned.

[0024] Example 1: Screening of Reactive Oxygen Scavenging Substances and Optimal Application Concentrations under High Temperature Heat Damage. Seedlings were raised in a greenhouse on March 25, 2025. When the seedlings had two leaves and one bud, they were placed in an artificial climate chamber for high temperature treatment (day / night temperature 40 / 25 ℃), with a photonic flux density (PFD) of 600 μmol•m. -2 • s -1 The treatment at room temperature (28 / 18 ℃) served as a control. MDA levels in the leaves were measured at 0, 24, and 72 h after treatment.

[0025] 24 hours after rice is subjected to stress, spray the following substances on the leaves, spraying evenly once on both sides of the leaves.

[0026] Experiment 1: Ectocin was taken at weight fractions of 0.5%, 1%, 2%, 4%, 8%, 16%, and 32%, respectively, and diluted 2000 times to prepare experimental groups with concentrations of 2.5 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 40 mg / L, 80 mg / L, and 160 mg / L.

[0027] The foliar spraying with an equal amount of water was set as a blank control for room temperature (28 / 18 ℃) (CK (room temperature)) and high temperature (40 / 25 ℃) (CK (high temperature)). Salicylic acid with a concentration of 80 mg / L was set as a routine control.

[0028] The results are as follows Figure 1 As shown in the figure, the results indicate that ectoine at concentrations of 40-1600 mg / L had a significant effect on reducing MDA content in rice leaves after 48 hours of high-temperature stress. Furthermore, at a concentration of 80 mg / L, ectoine was significantly superior to salicylic acid.

[0029] Experiment 2: Sugar alcohol calcium (calculated by calcium element content) with weight fractions of 2.6%, 5.2%, 10.6%, 21.1%, 42.2%, and 84.5% was diluted 2000 times to prepare experimental groups with calcium concentrations of 31.25 mg / L, 62.5 mg / L, 125 mg / L, 250 mg / L, 500 mg / L, and 1000 mg / L, respectively.

[0030] The foliar spray with equal amounts of water was set as a blank control at room temperature (28 / 18 ℃) and high temperature (40 / 25 ℃). The calcium concentration was set at 250 mg / L. The raw materials used were calcium nitrate, calcium chloride, and EDTA-calcium as conventional controls.

[0031] The results are as follows Figure 2 As shown in the figure, the results indicate that the experimental groups using sugar alcohol calcium with calcium concentrations of 62.5-1000 mg / L significantly reduced the MDA content in rice leaves after 48 hours of high-temperature stress. Furthermore, at calcium concentrations of 250-1000 mg / L, the sugar alcohol calcium experimental group showed significantly better results than calcium nitrate, calcium chloride, and EDTA-calcium.

[0032] Experiment 3 On June 20, 2025, based on Experiments 1 and 2, a synergistic effect test was conducted using ectoine at a concentration of 40 mg / L and calcium at a concentration of 250 mg / L (sugar alcohol calcium).

[0033] The experimental groups selected were: ectoine concentration of 40 mg / L, calcium concentration of 125 mg / L (sugar alcohol calcium), and ectoine concentration of 20 mg / L + calcium concentration of 62.5 mg / L (sugar alcohol calcium), foliar spray.

[0034] A foliar spray was used as a routine control, with a salicylic acid concentration of 40 mg / L and a calcium concentration of 125 mg / L (calcium nitrate).

[0035] The leaf spraying group with an equal amount of water was set as a blank control at room temperature (28 / 18 ℃) and high temperature (40 / 25 ℃).

[0036] The MDA content in rice leaves was measured after 48 hours of high temperature stress.

[0037] The results are as follows Figure 3 As shown in the figure, the experimental group with ectoine concentration of 20 mg / L + calcium concentration of 62.5 mg / L (sugar alcohol calcium) was significantly better than the experimental group with ectoine concentration of 40 mg / L, calcium concentration of 125 mg / L (sugar alcohol calcium), and salicylic acid concentration of 80 mg + calcium concentration of 125 mg / L (calcium nitrate) in reducing the MDA content in rice leaves after 48 hours of high temperature stress.

[0038] Furthermore, based on the heat damage symptoms of the seedlings, the heat damage of each group was classified, and the heat damage index was calculated according to the following formula.

[0039] Heat damage index = ∑(heat damage level of each level × number of plants at each level) / (highest level × total number of plants in the sample) × 100%.

[0040] The results are as follows Figure 4 As shown in the figure, the results indicate that the experimental group with ectoine concentration of 20 mg / L + calcium concentration of 62.5 mg / L (sugar alcohol calcium) was significantly better than the experimental group with ectoine concentration of 40 mg / L, calcium concentration of 125 mg / L (sugar alcohol calcium), and salicylic acid concentration of 80 mg + calcium concentration of 250 mg / L (calcium nitrate) in reducing the heat injury index of rice.

[0041] Example 2: Experiment on the effects of T6P on empty grains and yield of rice caused by high temperature heat damage On August 10, 2025, in Xinjian County, Jiangxi Province, foliar spraying with different concentrations of T6P was conducted two days after rice heat damage (temperature > 35℃). On October 13, 2025, the rate of empty grains was counted and the yield was measured. The results are shown in Tables 1 and 2 below.

[0042] T6P with weight fractions of 0.25%, 0.5%, 1%, 2%, 4%, 8%, and 16% was diluted 2000 times to prepare experimental groups with concentrations of 1.25 mg / L, 2.5 mg / L, 5 mg / L, 10 mg / L, 20 mg / L, 40 mg / L, and 80 mg / L, respectively.

[0043] The experimental groups selected in Experiment 3 of Example 1 consisted of calcium concentrations of 62.5 mg / L (sugar alcohol calcium) and ectoine concentrations of 20 mg / L.

[0044] An experimental group with a trehalose concentration of 80 mg / L was set up as a control.

[0045] An equal volume of water was sprayed onto the leaves as a blank control (CK).

[0046] Table 1

[0047] As shown in Table 1, the experimental groups with T6P concentrations of 5-80 mg / L had a significant effect on reducing the rate of empty grains in heat-damaged rice, decreasing the rate by approximately 2.3-6.5%. The experimental group with a T6P concentration of 80 mg / L was significantly better than the experimental group with a trehalose concentration of 80 mg / L, and the experimental group with a T6P concentration of 80 mg / L was significantly better than the experimental group with an ectoine concentration of 20 mg / L + a calcium concentration of 62.5 mg / L (sugar alcohol calcium). The experimental group with an ectoine concentration of 20 mg / L + a calcium concentration of 62.5 mg / L (sugar alcohol calcium) was better than the experimental group with a trehalose concentration of 80 mg / L.

[0048] Table 2

[0049] As shown in Table 2, the experimental groups with T6P concentrations of 5-80 mg / L had a significant effect on increasing the yield of rice affected by heat damage, increasing the yield by approximately 20.5-50 kg / mu (667 square meters), with a yield increase rate of approximately 3.8-9.3%. The experimental group with a T6P concentration of 10 mg / L was significantly better than the experimental group with a trehalose concentration of 80 mg / L, and the experimental group with a T6P concentration of 40 mg / L was significantly better than the experimental group with an ectoine concentration of 20 mg / L + a calcium concentration of 62.5 mg / L (sugar alcohol calcium). The experimental group with an ectoine concentration of 20 mg / L + a calcium concentration of 62.5 mg / L (sugar alcohol calcium) was significantly better than the experimental group with a trehalose concentration of 80 mg / L.

[0050] Example 3: Experiment on the effects of T6P, sugar alcohol calcium, and ectoine combination on empty grains and yield in rice affected by high temperature heat damage. Based on the screening results of Example 2, a combination of three components, T6P, sugar alcohol calcium, and ectoine (hereinafter referred to as the T6P composition), was developed and tested at different concentrations on rice affected by heat damage.

[0051] On August 1, 2025, in Zhijiang City, Hubei Province, foliar spraying of different concentrations of the compound was carried out 5 days after the occurrence of rice heat injury (temperature > 38℃). On September 28, 2025, the empty grain rate and yield were measured, and the results are shown in Tables 3 and 4 below.

[0052] The experimental groups were prepared with the following total concentrations of T6P compositions (i.e., three compositions): 30.4 mg / L (i.e., the weight fractions of T6P, ectoine, and calcium tartrate were 1.2%, 1.2%, and 1.5%, respectively), 45.5 mg / L (i.e., the weight fractions of T6P, ectoine, and calcium tartrate were 1.8%, 1.8%, and 2.3%, respectively), 68.3 mg / L (i.e., the weight fractions of T6P, ectoine, and calcium tartrate were 2.7%, 2.7%, and 3.5%, respectively), and 102.5 mg / L (i.e., the weight fractions of T6P, ectoine, and calcium tartrate were 1.8%, 1.8%, and 2.3%, respectively). The concentrations were 4%, 4%, and 5.2% (i.e., the weight fractions of T6P, ectoine, and sugar alcohol calcium were 6%, 6%, and 7.8%, respectively), and 230.6 mg / L (i.e., the weight fractions of T6P, ectoine, and sugar alcohol calcium were 9%, 9%, and 11.7%, respectively). Among them, 102.5 mg / L was T6P concentration of 20 mg / L + ectoine concentration of 20 mg / L + sugar alcohol calcium concentration of 62.5 mg / L. The other groups were set with the same proportions, with gradients in multiples of 1.5, such as 68.3 mg / L, which was T6P concentration of 13.3 mg / L, ectoine concentration of 13.3 mg / L, and calcium concentration of 41.7 mg / L.

[0053] The following control groups were set up: a combination of trehalose concentration of 40 mg / L, calcium nitrate concentration of 125 mg / L, and salicylic acid concentration of 40 mg / L (referred to as the salicylic acid control group); a combination of ectoine concentration of 20 mg / L and calcium concentration of 62.5 mg / L (sugar alcohol calcium) (referred to as the ectoine + calcium two-component control group); and a blank control (CK) group with an equal amount of water sprayed on the leaves.

[0054] Table 3

[0055] As shown in Table 3, the T6P composition began to work in reducing the rate of empty grains caused by heat damage in rice at a concentration of 30.4 mg / L. At a concentration of 45.5 mg / L, the reduction was comparable to that of the salicylic acid control group. The effect was better than that of the ectoine + calcium dual-component control group at a concentration of 68.3 mg / L. It had a significant effect at concentrations above 102.5 mg / L, reducing the rate of empty grains by more than 10.45%, which was significantly better than that of the salicylic acid control group and the ectoine + calcium dual-component control group.

[0056] Table 4

[0057] As shown in Table 4, the T6P composition began to exert its effect on increasing the yield of rice affected by heat damage at a concentration of 30.4 mg / L. At a concentration of 45.5 mg / L, the reduction was comparable to that of the salicylic acid control group. At a concentration of 68.3 mg / L and above, the effect was superior to that of the ectoine + calcium dual-component control group. At a concentration of 102.5 mg / L and above, it had a significant effect, increasing the yield by more than 61.6 kg / mu, with a yield increase rate of more than 12.6%, which was significantly better than the salicylic acid control group and the ectoine + calcium dual-component control group.

[0058] Example 4: Experiment on the effects of different proportions of compound products on empty grains and yield in rice affected by high temperature heat damage According to the above embodiments, different concentrations of T6P, which promotes nutrient transport, combined with ectoin, which scavenges reactive oxygen species and protects leaf cells, and sugar alcohol calcium (hereinafter referred to as the T6P composition) were tested on heat-damaged rice.

[0059] On August 18, 2025, in Yongxing, Hunan Province, foliar spraying treatments with different concentrations of the composition were conducted 7 days after rice heat injury (temperature > 36℃). Specifically, according to the components in the above examples, T6P at concentrations of 1.25 mg / L, 5 mg / L, 20 mg / L, and 80 mg / L (i.e., T6P weight fractions of 0.25%, 1%, 4%, and 16%, respectively), ectoine at concentrations of 2.5 mg / L, 80 mg / L, and 160 mg / L (i.e., ectoine weight fractions of 0.5%, 16%, and 32%, respectively), and sugar alcohol calcium at concentrations of 31.25 mg / L, 250 mg / L, 500 mg / L, and 1000 mg / L (i.e., calcium weight fractions of 2.6%, 21.1%, 42.2%, and 84.5%, respectively) were combined to form test groups 1-4 in the table below, and an equal volume of water was set up as a blank control (CK).

[0060] On October 9, 2025, the empty grain rate was calculated and the yield was measured. The results are shown in Tables 5 and 6 below.

[0061] Table 5

[0062] Table 6

[0063] The experimental results show that different combinations of T6P with ectoine and sugar alcohol calcium can effectively reduce the rate of empty grains in rice under heat damage conditions and increase yield in the field.

[0064] Fertilizer Formulation and Application: Referring to the above embodiments, when formulating the specific fertilizer product, weigh the following components of the T6P composition: 0.25-16 parts by weight of trehalose-6-phosphate (T6P), 0.5-30 parts by weight of ectoine, and 2.6-85 parts by weight of chelated calcium (based on the weight of the calcium element contained therein). Pass each component through a 100-mesh sieve to ensure uniform particle size. Add 0.1-1 parts by weight of xanthan gum to improve stability; and 0.1-5 parts by weight of propylene glycol and 0.05-0.1 parts by weight of potassium sorbate as antifreeze agents. Fill the remaining volume (total volume 100 parts by weight) with sodium sulfate as a filler, and then mix for 30 minutes to ensure uniform mixing of all components. For application, the solution can be diluted with water at different ratios for foliar spraying, such as 2000 times or 3000 times.

[0065] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition, characterized in that, Based on 100 parts by weight of the total weight of the composition, the composition comprises: more than 0.25 parts by weight of trehalose-6-phosphate, more than 0.5 parts by weight of ectoine, and more than 2.6 parts by weight of chelated calcium, wherein the weight of the chelated calcium is based on the weight of the calcium contained therein.

2. The composition according to claim 1, characterized in that, Based on 100 parts by weight of the total weight of the composition, the composition comprises: more than 1 part by weight of trehalose-6-phosphate, more than 8 parts by weight of ectoine, and more than 5.2 parts by weight of chelated calcium.

3. The composition according to claim 1, characterized in that, Based on 100 parts by weight of the total composition, the composition comprises: 1-16 parts by weight of trehalose-6-phosphate, 8-32 parts by weight of ectoine, and 5.2-85 parts by weight of chelated calcium.

4. The composition according to claim 1, characterized in that, The chelated calcium is selected from one or more of sugar alcohol calcium, EDTA-calcium, amino acid chelated calcium, and DTPA chelated calcium.

5. The composition according to claim 4, characterized in that, The chelated calcium is sugar alcohol calcium.

6. The composition according to claim 1, characterized in that, The composition also includes a commonly used fertilizer formulation, which optionally is selected from one or more of xanthan gum, polyvinylpyrrolidone, glycerol, propylene glycol, potassium sorbate, formaldehyde, sodium diacetate, and water.

7. A product characterized in that, The composition comprising any one of claims 1-6, wherein the product is a biostimulant, fertilizer, crop antibacterial agent, or soil conditioner.

8. The product according to claim 7, characterized in that, Its dosage forms include soluble powder, wettable powder, emulsifiable concentrate, oil suspension, microcapsule or suspension concentrate.

9. The use of the composition according to any one of claims 1-6 in any of the following aspects: (1) Promote crop growth, increase crop yield and / or quality; (2) Reduce empty grains in crops and increase their plumpness; (3) Reduce yellowing and wilting of crop leaves; (4) Preventing and controlling high-temperature heat damage to crops.

10. The application according to claim 9, characterized in that, The crops mentioned include: cereal crops, legume crops, fruits and vegetables.

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