Method for preparing amino acid water-soluble fertilizer by processing animal blood

Abstract

The invention discloses a method for preparing an amino acid water-soluble fertilizer by processing animal blood, which belongs to the technical field of water-soluble fertilizer preparation, and comprises the following steps: step 1, collecting animal blood, and performing centrifugal treatment to remove part of impurities and blood cell fragments in the blood to obtain a relatively pure plasma part; 2, compound protease is added into the pretreated animal blood for an enzymolysis reaction, meanwhile, sodium selenite is added into an enzymolysis system, and the sodium selenite serves as a selenium source; 3, after enzymolysis is completed, a compound microbial agent is added into enzymatic hydrolysate, and aerobic fermentation is carried out; according to the method for preparing the amino acid water-soluble fertilizer through animal blood processing, the structural design is reasonable, the added selenium element can enhance the stress resistance of plants and improve the quality of agricultural products, and an acidolysis method or an enzymolysis method and a liquid fertilizer or solid fertilizer production mode are flexibly selected, so that the optimal economic benefits and social benefits are achieved.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of water-soluble fertilizer preparation, in particular to a method for preparing amino acid water-soluble fertilizer from animal blood. BACKGROUND

[0002] As a kind of efficient, environment-friendly and comprehensive nutrient fertilizer for plants, the amino acid water-soluble fertilizer has been widely used in the agricultural field in recent years. The traditional preparation method of the amino acid water-soluble fertilizer is to use animal and plant proteins as raw materials, convert the animal and plant proteins into amino acids through hydrolysis, and then compound the amino acids with other nutrient elements.

[0003] The traditional preparation raw materials of the amino acid water-soluble fertilizer are mainly animal hairs and soybean meal, while the animal blood, as a kind of resource rich in proteins, is often wasted or not fully utilized. Meanwhile, the existing amino acid water-soluble fertilizer still needs to be improved in terms of comprehensiveness and functionality of nutrient components, especially the addition and application of selenium, a kind of trace element which plays an important role in plant growth and human health. Therefore, the method for preparing amino acid water-soluble fertilizer from animal blood is proposed. SUMMARY

[0004] The application aims to provide the method for preparing amino acid water-soluble fertilizer from animal blood, so as to solve the problems in the background.

[0005] To achieve the above-mentioned purpose, the application provides the following technical scheme: the method for preparing amino acid water-soluble fertilizer from animal blood comprises the following steps: Step one: collect animal blood, and first perform centrifugal treatment to remove part of impurities and blood cell fragments in the blood, so as to obtain a relatively pure plasma part; Step two: add a compound protease to the pretreated animal blood to perform enzymatic reaction, and at the same time, add sodium selenite to the enzymatic system, and the sodium selenite is used as a selenium source; Step three: after the enzymatic reaction is completed, add a compound microbial agent to the enzymatic solution to perform aerobic fermentation; Step four: after the fermentation is completed, filter the fermentation liquor to remove microbial bodies and incomplete decomposition impurities, so as to obtain a clear liquid; Step five: perform quality detection on the prepared selenium-rich amino acid water-soluble fertilizer, and after the detection is qualified, perform filling, so as to obtain a finished product of the selenium-rich amino acid water-soluble fertilizer.

[0006] Preferably, the blood pretreatment in step one is performed. Animal blood collection: collect fresh animal blood such as pig blood and chicken blood, and ensure that the blood is not contaminated and not deteriorated. The subsequent treatment is performed within 2 hours after the blood is collected, so as to prevent the blood from coagulating and microorganisms from breeding. Impurity removal: the collected animal blood is filtered through a 200-mesh filter screen to remove large-particle impurities such as hair and tissue fragments, and then the filtered blood is placed in a centrifuge and centrifuged at 3000 rpm for 15 minutes to further remove blood cell precipitates and fat layers. Blood dilution: deionized water is added to the supernatant of the centrifuged blood, and the blood is diluted at a volume ratio of 1:2 to reduce the viscosity of the blood and facilitate subsequent hydrolysis.

[0007] Preferably, the enzyme hydrolysis reaction of step two; Reagent addition: the diluted blood is transferred to an acid-resistant reaction kettle, and a 10% sulfuric acid solution is added, with the amount of sulfuric acid solution being 15% of the volume of the blood. Sulfuric acid acts as a hydrolysis catalyst to promote the decomposition of proteins in the blood into amino acids. Reaction conditions: nitrogen gas is introduced into the reaction kettle to exclude air and prevent oxidation of the components in the blood. Then, the temperature of the reaction kettle is raised to 110°C and maintained at this temperature for 4 hours. During the reaction, continuous stirring is required at a speed of 200 rpm to ensure uniform reaction.

[0008] Preferably, enzyme preparation selection: if enzyme hydrolysis is used, a mixed enzyme preparation of papain and neutral protease can be selected, with the amount of enzyme preparation being 5% of the dry weight of the blood. Reaction conditions: first adjust the pH of the diluted blood to 7.0 using a sodium hydroxide solution or hydrochloric acid solution, then control the temperature of the reaction kettle at 45°C, add the enzyme preparation, and react for 24 hours. During the enzyme hydrolysis process, continuous stirring is also required at a speed of 150 rpm. Preferably, selenium source selection: sodium selenite is selected as the source of selenium addition. Sodium selenite is easily soluble in water and has good stability. Addition amount and method: according to the standard of 0.05% selenium content in the finished fertilizer, calculate the required amount of sodium selenite, dissolve sodium selenite in a small amount of deionized water to prepare a 10% sodium selenite solution, then slowly add it to the prepared amino acid solution while continuously stirring to ensure uniform dispersion of selenium in the solution.

[0009] Preferably, the microbial fermentation of step three, during the fermentation process, the microorganisms further decompose the residual macromolecules after enzyme hydrolysis, making the nutrient components such as amino acids more abundant, and the metabolic activity of the microorganisms promotes the conversion of selenium in sodium selenite to organic selenium, improving the bioavailability of selenium. During the fermentation process, the temperature (30-35°C) and dissolved oxygen conditions need to be controlled to ensure the activity of the microorganisms.

[0010] Preferably, the filtering and preparation of step four; Neutralization reaction: after the hydrolysis reaction is completed, the reaction solution is cooled to room temperature, and 20% sodium hydroxide solution is slowly added dropwise to the reaction solution to neutralize the remaining sulfuric acid. The pH value of the reaction solution is adjusted to 6.5. During the neutralization process, constant stirring is required to ensure that the neutralization reaction proceeds completely. Filtering and slag removal: the neutralized reaction solution is filtered through a plate and frame filter press with a filter cloth aperture of 50 microns to remove unhydrolyzed impurities and precipitates, and the obtained filtrate is the preliminary amino acid solution.

[0011] Preferably, the filtered amino acid solution is transferred to a vacuum concentration tank and concentrated under reduced pressure at a vacuum degree of -0.08 MPa and a temperature of 60°C to increase the concentration of the amino acid solution to 15% (dry basis). According to the needs, the following nutrient elements are added to the concentrated amino acid solution: nitrogen source: urea is added to make the nitrogen content in the fertilizer reach 10% (N). Phosphorus source: potassium dihydrogen phosphate is added to make the phosphorus content in the fertilizer reach 5% (P2O5).

[0012] Potassium source: potassium sulfate is added to make the potassium content in the fertilizer reach 8% (K2O). Trace elements: chelated iron, zinc, boron and other trace elements are added, with a total amount of 3% of the weight of the fertilizer. The specific formulation is: chelated iron 1%, chelated zinc 1%, chelated boron 0.5%, chelated manganese 0.5%, to prepare a balanced nutrient selenium-rich amino acid water-soluble fertilizer.

[0013] Preferably, liquid fertilizer preparation: the amino acid solution with added selenium elements and other nutrient components is subjected to homogenization treatment by a homogenizer at a pressure of 20 MPa to ensure the uniformity of the fertilizer components, and then the homogenized liquid fertilizer is filled and packaged in plastic barrels or plastic bottles, with a capacity of each barrel (bottle) determined according to market demand, such as 5 liters, 10 liters, etc. Solid fertilizer preparation: if solid fertilizer is to be prepared, the prepared amino acid solution can be subjected to spray drying by a spray drying tower. The inlet air temperature for spray drying is 180°C, and the outlet air temperature is 80°C. The dried powder is then granulated by a granulator to form granular fertilizer with a particle size of 2-4 mm. After screening, unqualified granules are removed, and then the granular fertilizer is packaged, usually in plastic bags or woven bags, with a weight of each bag being usually 25 kg or 50 kg.

[0014] Preferably, raw material detection: during the collection of animal blood, the source, freshness and microbial indicators of the blood are strictly detected. Process monitoring: In the process of hydrolysis, neutralization, concentration, formulation, etc., the reaction conditions (such as temperature, pH value, reaction time, etc.) are monitored and recorded in real time to ensure the stability of the process parameters; Finished product detection: The nutrient content (such as amino acid content, nitrogen, phosphorus and potassium content, selenium content, etc.), pH value, heavy metal content, etc. of the finished fertilizer are comprehensively detected.

[0015] Compared with the prior art, the animal blood processing method for preparing amino acid water-soluble fertilizer has the following advantages: compared with the traditional animal blood processing method for preparing amino acid water-soluble fertilizer, sodium selenite is introduced in the enzymolysis stage, and microbial fermentation is combined to further promote the conversion of selenium, so that the selenium element is better combined with nutrient ingredients such as amino acids, the organic degree of selenium and the functionality of biological fertilizer are improved, the waste resource of animal blood is fully utilized, the production cost is reduced, and through scientific process design and nutrient deployment, the fertilizer has higher nutritional value and biological activity, the added selenium element can enhance the stress resistance of plants and improve the quality of agricultural products, has a wide market application prospect, and in the actual production process, the acidolysis method or the enzymolysis method, and the production mode of liquid fertilizer or solid fertilizer can be flexibly selected according to different needs and conditions to realize the best economic benefit and social benefit. BRIEF DESCRIPTION OF DRAWINGS

[0016] Figure 1 The flow chart of the animal blood processing method for preparing amino acid water-soluble fertilizer is shown in the figure. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present application will be described clearly and completely in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application. EMBODIMENT

[0018] Please refer to Figure 1 The present application provides a technical solution: an animal blood processing method for preparing amino acid water-soluble fertilizer. In this embodiment, fresh pig blood is used as raw material, and a compound protease enzymolysis process is adopted to prepare a liquid selenium-rich amino acid water-soluble fertilizer with high biological effectiveness, which comprises the following steps: Step one: collect animal blood, first centrifugal treatment, remove part of impurities and blood cell fragments in blood, get relatively pure plasma part; Collect 10L of fresh blood of healthy pigs, process within 1.5 hours after collection, filter through a 200-mesh screen to remove hair and tissue fragments; The filtered blood is placed in a centrifuge and centrifuged at 3000 r / min for 15 minutes. The blood cell precipitate and the upper fat layer are discarded, and 5 L of plasma supernatant is retained. 10 L of deionized water is added for dilution at a volume ratio of plasma:deionized water = 1:2 to reduce the viscosity.

[0019] Step two: complex protease is added to the pretreated animal blood for enzymatic reaction. At the same time, sodium selenite is added to the enzymatic system as a selenium source. The pH of the diluted plasma is adjusted to 7.0 with 1 mol / L sodium hydroxide solution, and it is transferred to a 50 L acid-resistant reaction kettle. Complex protease (papain: neutral protease = 1:1 mixture, amounting to 5% of the dry weight of the plasma, about 200 g) is added. The reaction temperature is set to 45°C, the stirring speed is 150 r / min, and the enzymolysis time is 24 hours. At the same time, sodium selenite is dissolved in deionized water to prepare a 10% solution (according to the selenium content of 0.05% in the finished product, 50 g of sodium selenite needs to be added), which is slowly added to the enzymolysis solution and continuously stirred.

[0020] Step three: After the enzymolysis is completed, complex microbial agents are added to the enzymolysis solution for aerobic fermentation. Complex microbial agents (Bacillus subtilis: yeast = 2:1, amounting to 2% of the volume of the enzymolysis solution) are added to the enzymolysis solution. The fermentation temperature is controlled at 32°C, sterile air is introduced to maintain the dissolved oxygen content at 2-3 mg / L, and the fermentation time is 48 hours.

[0021] Step four: After the fermentation is completed, the fermentation broth is filtered to remove microbial cells and incompletely decomposed impurities, obtaining a clear liquid. After the fermentation is completed, the pH is neutralized to 6.5 with 20% sodium hydroxide solution, and the filtrate is collected by filtering through a 50 μm plate frame filter press. The filtrate is transferred to a vacuum concentration tank and concentrated to an amino acid concentration of 15% (dry basis) at -0.08 MPa and 60°C. Nutritional ingredients are added in the following proportions: urea (to make the N content reach 10%), potassium dihydrogen phosphate (to make the P2O5 content reach 5%), potassium sulfate (to make the K2O content reach 8%), and chelated trace elements (chelated iron 1%, chelated zinc 1%, chelated boron 0.5%, chelated manganese 0.5%). After 20 MPa homogenization treatment, the ingredients are evenly distributed.

[0022] Step five: The quality of the prepared selenium-rich amino acid water-soluble fertilizer is detected, and after passing the detection, it is filled to obtain the finished product of selenium-rich amino acid water-soluble fertilizer.

[0023] The finished product is detected: the amino acid content is greater than or equal to 15%, N-P2O5-K2O is greater than or equal to 23%, the selenium content is 0.05%, the pH is 6.5, and the heavy metals (lead, cadmium, and mercury) are all less than or equal to 0.1 mg / kg, which meets the standard; Filling in a 10L plastic bucket, and after sealing, it is the finished product Embodiment

[0024] Please refer to Figure 1 The present application provides a technical solution: a method for preparing amino acid water-soluble fertilizer from animal blood. In this embodiment, chicken blood is used as raw material, and the hydrolysis efficiency is improved by using sulfuric acid hydrolysis method. The solid particle fertilizer prepared by spray drying is convenient for storage and transportation, and comprises the following steps: Step one: collect animal blood, first centrifugal treatment, remove part of impurities and blood cell fragments in the blood, get relatively pure plasma part; Collect fresh chicken blood 8L, filter through a 200 mesh screen within 2 hours, centrifuge at 3000 r / min for 15 minutes, and reserve 4L of plasma supernatant; Dilute with 8L deionized water at a volume ratio of 1:2.

[0025] Step two: add compound protease to the pretreated animal blood for enzymatic reaction, and at the same time, add sodium selenite to the enzymatic system as a selenium source; Transfer the diluted plasma into the reaction kettle, add 10% sulfuric acid solution (the amount is 15% of the volume of the plasma, i.e. 1.8L), and blow in nitrogen to remove air; Heat to 110℃, stirring speed 200 r / min, reaction for 4 hours; After the reaction is completed, cool to 60℃, add 10% sodium selenite solution (calculate the selenium content of the finished product as 0.05%, add 40g of sodium selenite), and stir evenly.

[0026] Step three: after the enzymatic reaction is completed, add compound microbial agent to the enzymatic solution for aerobic fermentation; Add compound microbial agent (lactic acid bacteria: actinomycetes = 1:1, the amount is 3% of the volume of the acid hydrolysis solution), control the temperature at 30℃, the dissolved oxygen at 1.5-2.5 mg / L, and ferment for 36 hours.

[0027] Step four: after the fermentation is completed, filter the fermentation liquor to remove microbial bodies and incomplete decomposition impurities, and obtain clear liquid; After neutralization to pH=6.5, filter through a 50μm plate frame, and concentrate the filtrate to an amino acid concentration of 18% at-0.08 MPa and 60℃; Add urea, potassium dihydrogen phosphate, potassium sulfate, and chelated trace elements according to the nutrient component ratio of embodiment 1, and stir evenly; The dispensing solution is sent into a spray drying tower, the inlet air temperature is 180 DEG C, and the outlet air temperature is 80 DEG C, so that amino acid powder is obtained; The powder is granulated by a granulator, 2-4mm granules are prepared, and unqualified granules are removed by screening.

[0028] Step five: quality detection is performed on the prepared selenium-rich amino acid water-soluble fertilizer, and after the detection is qualified, the selenium-rich amino acid water-soluble fertilizer is obtained.

[0029] The product is detected: the amino acid content is greater than or equal to 18%, the N-P2O5-K2O is greater than or equal to 23%, the selenium content is 0.05%, and the moisture content is less than or equal to 5%, which meets the standard; The product is packaged in a 25kg woven bag, and after sealing, it is a solid granular product. Embodiment

[0030] Please refer to Figure 1 The present application provides a technical solution: a method for preparing amino acid water-soluble fertilizer from animal blood, the present embodiment uses mixed raw materials of pig blood and chicken blood, simplifies the fermentation step, reduces the production cost, and is suitable for small and medium-sized production scenes, including the following steps: Step one: collect animal blood, first centrifugal treatment, remove part of impurities and blood cell fragments in the blood, get relatively pure plasma part; Mix fresh pig blood 5L, chicken blood 5L, a total of 10L, filter through a 200 mesh screen within 2 hours, centrifuge at 3000r / min for 15 minutes, and reserve 5L of mixed blood plasma; Dilute with 10L deionized water at a volume ratio of 1:2.

[0031] Step two: add compound protease to the pretreated animal blood for enzymolysis reaction, and at the same time, add sodium selenite to the enzymolysis system, and sodium selenite is used as a selenium source; Adjust the pH to 7.0, add a single neutral protease (the amount is 4% of the dry weight of the plasma, about 160g), and enzymolysis at 45 DEG C, 150r / min for 20 hours; Add 10% sodium selenite solution (50g sodium selenite), and stir uniformly.

[0032] Step three: after the enzymolysis is completed, add a compound microbial agent to the enzymolysis liquid for aerobic fermentation. Omit the compound microbial agent fermentation, directly neutralize the enzymolysis liquid to pH=6.5, filter through a 50um plate frame, and collect the filtrate.

[0033] Step four: after the fermentation is completed, filter the fermentation liquid to remove microbial bodies and incomplete decomposition impurities, and obtain a clear liquid. The filtrate does not need to be concentrated, and urea (N content 10%), potassium dihydrogen phosphate (P2O5 content 5%), and potassium sulfate (K2O content 8%) are directly added, and chelated trace elements are omitted. 20 MPa homogenization treatment.

[0034] Step five: quality detection is performed on the prepared selenium-rich amino acid water-soluble fertilizer, and after the detection is qualified, the selenium-rich amino acid water-soluble fertilizer is filled to obtain a finished product.

[0035] The finished product is detected: the amino acid content is ≥8%, the N-P2O5-K2O is ≥23%, and the selenium content is 0.05%, which meets the basic water-soluble fertilizer standard; The finished product is filled in 5L plastic bottles as an economic product.

[0036] It should be noted that, in this text, relational terms such as first and second are used merely to distinguish one entity or action from another, without necessarily requiring or implying any such actual relationship or order between or among the entities or actions. Moreover, the terms "comprises", "comprising", or any other variations thereof are intended to cover non-exclusive inclusions, so that a process, method, article, or apparatus that comprises a list of elements does not only include those elements, but also includes other elements not expressly listed or inherent to such a process, method, article, or apparatus.

[0037] Although the present application has been described above with reference to the embodiments, various modifications can be made thereto and equivalents can be substituted for elements thereof without departing from the scope of the present application. In particular, features of the disclosed embodiments can be combined together in any manner without structural conflict, and the combinations are not exhaustively described in the specification only for the purpose of omitting the length and saving resources. Therefore, the present application is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A method for preparing amino acid water-soluble fertilizer from animal blood, characterized in that: Includes the following steps: Step 1: Collect animal blood and centrifuge it to remove some impurities and blood cell fragments, obtaining a relatively pure plasma portion; Step 2: Add a complex protease to the pretreated animal blood for enzymatic hydrolysis. At the same time, add sodium selenite to the enzymatic hydrolysis system as a selenium source. Step 3: After enzymatic hydrolysis is complete, add compound microbial inoculant to the hydrolysate and carry out aerobic fermentation; Step 4: After fermentation is complete, filter the fermentation liquid to remove microbial cells and incompletely decomposed impurities, and obtain a clear liquid; Step 5: Conduct quality testing on the prepared selenium-enriched amino acid water-soluble fertilizer. After passing the test, it will be bottled to obtain the finished selenium-enriched amino acid water-soluble fertilizer.

2. The method for preparing amino acid water-soluble fertilizer from animal blood according to claim 1, characterized in that: The blood pretreatment described in step one; Animal blood collection: Collect fresh animal blood, such as pig blood and chicken blood, to ensure that the blood is uncontaminated and unspoiled. Follow-up processing is carried out within 2 hours after blood collection to prevent blood clotting and microbial growth. Impurity removal: The collected animal blood is filtered through a 200-mesh filter to remove large particles such as hair and tissue fragments. Then, the filtered blood is placed in a centrifuge and centrifuged at 3000 rpm for 15 minutes to further remove blood cell sediment and fat layer. Blood dilution: Deionized water is added to the supernatant of centrifuged blood at a volume ratio of 1:2 (blood to water) to reduce the viscosity of the blood and facilitate subsequent hydrolysis.

3. The method for preparing amino acid water-soluble fertilizer from animal blood according to claim 1, characterized in that: The enzymatic hydrolysis reaction described in step two; Reagent addition: Transfer the diluted blood to an acid-resistant reactor and add a 10% sulfuric acid solution. The amount of sulfuric acid solution used is 15% of the blood volume. Sulfuric acid acts as a hydrolysis catalyst, which can promote the decomposition of proteins in the blood into amino acids. Reaction conditions: Nitrogen gas is introduced into the reaction vessel to purge air and prevent the components in the blood from being oxidized; then, the temperature of the reaction vessel is raised to 110°C and maintained at this temperature for 4 hours. During the reaction, the vessel is continuously stirred at a speed of 200 rpm to ensure that the reaction proceeds uniformly.

4. The method for preparing amino acid water-soluble fertilizer from animal blood according to claim 3, characterized in that: Enzyme selection: If enzymatic hydrolysis is used, a complex protease, such as a mixture of papain and neutral protease, can be selected. The amount of enzyme used is 5% of the dry weight of blood. Reaction conditions: First, adjust the pH of the diluted blood to 7.0 using sodium hydroxide solution or hydrochloric acid solution. Then, control the temperature of the reaction vessel at 45℃, add the enzyme preparation, and react for 24 hours. During the enzymatic hydrolysis process, continuous stirring is required at a stirring speed of 150 rpm.

5. The method for preparing amino acid water-soluble fertilizer from animal blood according to claim 4, characterized in that: Selenium source selection: Sodium selenite was selected as the source of selenium. Sodium selenite is easily soluble in water and has good stability. Dosage and method of addition: Calculate the required amount of sodium selenite to be added according to the standard that the selenium content in the finished fertilizer reaches 0.05%. Dissolve the sodium selenite in a small amount of deionized water to prepare a 10% sodium selenite solution. Then slowly add it to the prepared amino acid solution while stirring continuously to ensure that the selenium element is evenly dispersed in the solution.

6. The method for preparing amino acid water-soluble fertilizer from animal blood according to claim 1, characterized in that: In step three, the microbial fermentation process involves the microorganisms further decomposing the macromolecular substances remaining after enzymatic hydrolysis, enriching the nutrients such as amino acids. On the other hand, the metabolic activities of the microorganisms promote the more complete conversion of selenium in sodium selenite into organic selenium, thereby improving the bioavailability of selenium. During the fermentation process, it is necessary to control the temperature (30-35℃), dissolved oxygen, and other conditions to ensure the activity of the microorganisms.

7. The method for preparing amino acid water-soluble fertilizer from animal blood according to claim 1, characterized in that: The filtering and blending described in step four; Neutralization reaction: After the hydrolysis reaction is completed, wait for the reaction solution to cool to room temperature, then slowly add 20% sodium hydroxide solution dropwise to the reaction solution to neutralize the remaining sulfuric acid and adjust the pH of the reaction solution to 6.

5. During the neutralization process, the solution should be stirred continuously to ensure that the neutralization reaction is fully carried out. Filtration and slag removal: The neutralized reaction solution is filtered through a plate and frame filter press with a filter cloth pore size of 50 micrometers to remove unhydrolyzed impurities and precipitates. The resulting filtrate is the preliminary amino acid solution.

8. The method for preparing amino acid water-soluble fertilizer from animal blood according to claim 7, characterized in that: The filtered amino acid solution was transferred to a vacuum concentration tank and concentrated under reduced pressure at a vacuum of -0.08 MPa and a temperature of 60°C to increase the concentration of the amino acid solution to 15% (on a dry basis). The following nutrients were added to the concentrated amino acid solution as needed: Nitrogen source: Add urea to make the nitrogen content in the fertilizer reach 10% (calculated as N). Phosphorus source: Add potassium dihydrogen phosphate to make the phosphorus content in the fertilizer reach 5% (calculated as P2O5); Potassium source: Add potassium sulfate to make the potassium content in the fertilizer reach 8% (calculated as K2O); Micronutrients: Add chelated micronutrients such as iron, zinc, and boron, totaling 3% of the fertilizer weight; the specific formula is: 1% chelated iron, 1% chelated zinc, 0.5% chelated boron, and 0.5% chelated manganese, to create a nutritionally balanced selenium-enriched amino acid water-soluble fertilizer.

9. The method for preparing amino acid water-soluble fertilizer from animal blood according to claim 1, characterized in that: Liquid fertilizer preparation: The amino acid solution with added selenium and other nutrients is homogenized using a homogenizer at a pressure of 20 MPa to ensure the uniformity of fertilizer components. Then, the homogenized liquid fertilizer is bottled and packaged in plastic buckets or bottles. The capacity of each bucket (bottle) can be determined according to market demand, such as 5 liters, 10 liters, etc. Solid fertilizer preparation: To prepare solid fertilizer, the prepared amino acid solution can be spray-dried through a spray drying tower. The inlet air temperature of the spray dryer is 180℃ and the outlet air temperature is 80℃. The dried powder is then granulated by a granulator to produce granular fertilizer with a particle size of 2-4 mm. After sieving, unqualified particles are removed, and then the fertilizer is packaged. Plastic bags or woven bags can be used for packaging, and each bag usually weighs 25 kg or 50 kg.

10. The method for preparing amino acid water-soluble fertilizer from animal blood according to claim 1, characterized in that: Raw material testing: During the animal blood collection stage, strict testing is conducted on the blood's source, freshness, and microbiological indicators; Process monitoring: Real-time monitoring and recording of reaction conditions (such as temperature, pH value, reaction time, etc.) are carried out at each process stage, including hydrolysis, neutralization, concentration, and blending, to ensure the stability of process parameters; Finished product testing: Comprehensive testing is conducted on the nutrient content (such as amino acid content, nitrogen, phosphorus, potassium content, selenium content, etc.), pH, and heavy metal content of the finished fertilizer.

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