Organ-like culture system and preparation method thereof
By using biocompatible scaffolds, microfluidic chips, and dynamic culture devices, the problems of static environment and insufficient scaffold materials in organoid culture have been solved, enabling efficient and stable culture of organoids, which is suitable for drug screening and regenerative medicine research of various organs.
Patent Information
- Application Number
- CN202511410436.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-29
- Publication Date
- 2026-02-27
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Existing organoid culture technologies suffer from problems such as static culture environment, insufficient scaffold materials, limited maturity, and short culture cycles, resulting in low nutrient exchange efficiency, unclear composition, incomplete functional differentiation, and poor long-term stability.
By employing biocompatible scaffolds, microfluidic chips, biodegradable microcarriers, and dynamic culture devices, combined with a monitoring module, the in vivo microenvironment is simulated. The maturity and functional differentiation of organoids are improved through three-dimensional porous scaffolds and dynamic fluid shear forces. The efficient exchange of nutrients and metabolic waste is achieved using microfluidic chips, and biodegradable microcarriers are used to match the tissue growth rate.
It significantly improves the maturity and functional differentiation of organoids, enhances culture efficiency and stability, reduces batch-to-batch variability, and is suitable for long-term culture of various organs and drug screening studies.
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Figure CN121574818A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application belongs to the field of biotechnology, and specifically relates to an organoid culture system and a preparation method thereof. BACKGROUND
[0002] An organoid is a three-dimensional cell aggregate formed by stem cells or progenitor cells in vitro, which has a structure and partial function similar to an in vivo organ, and has broad application prospects in basic research, drug development and regenerative medicine.
[0003] However, the existing organoid culture technology has the following problems: static culture environment: organoids in traditional culture dishes or plates lack dynamic fluid stimulation, resulting in low exchange efficiency of nutrients and metabolic waste.
[0004] Insufficient scaffold material: the commonly used Matrigel has problems such as large batch difference, unclear composition and animal origin, which affects the repeatability of culture results and the safety of clinical application.
[0005] Limited maturity: most in vitro cultured organoids have simple structure and incomplete functional differentiation, making it difficult to simulate the complex physiological functions of real organs.
[0006] Short culture period: the existing system is difficult to maintain the survival and functional stability of organoids for a long time, which limits long-term drug testing and disease progression research.
[0007] Therefore, it is of great significance to develop a culture system that can simulate the in vivo microenvironment and improve the maturity and long-term stability of organoids. SUMMARY
[0008] In view of the problems mentioned in the background, the purpose of the present application is to provide an organoid culture system and a preparation method thereof to solve the problems mentioned in the background.
[0009] The above technical purpose of the present application is realized by the following technical scheme: an organoid culture system, comprising a biocompatible scaffold, a microfluidic chip, a degradable microcarrier, a dynamic culture device and a monitoring module.
[0010] Preferably, the biocompatible scaffold is composed of a degradable polymer material and a bioactive factor, and has a three-dimensional porous structure.
[0011] Preferably, the microfluidic chip contains multiple culture chambers and microchannels, and can accurately control the flow of culture solution.
[0012] Preferably, the degradable microcarrier is loaded with stem cells or progenitor cells and can be gradually degraded during the culture process.
[0013] Preferably, the dynamic culture device can provide periodic fluid shear forces to simulate the hemodynamic environment in vivo.
[0014] Preferably, the monitoring module can monitor parameters such as pH value, dissolved oxygen and metabolite concentration in real time.
[0015] The present invention also discloses a method for preparing an organoid culture system, comprising the following steps: scaffold material synthesis: combining biodegradable polymer materials with bioactive factors to prepare a three-dimensional scaffold.
[0016] Microcarrier preparation: Cell-loaded biodegradable microcarriers were prepared using an emulsification-crosslinking method.
[0017] Microfluidic chip fabrication: Microfluidic chips are fabricated using photolithography and soft photolithography techniques.
[0018] System assembly: Integrating scaffolds, microcarriers, and microfluidic chips into a dynamic culture device.
[0019] Cell seeding and culture: Stem cells or progenitor cells are seeded onto microcarriers and cultured dynamically.
[0020] Preferably, the scaffold material synthesis step employs electrospinning or 3D printing technology.
[0021] Preferably, the microfluidic chip fabrication step uses PDMS material to fabricate the chip body.
[0022] Preferably, the flow rate and shear force of the culture medium in the dynamic culture step can be adjusted to simulate the physiological environment of different organs.
[0023] In summary, the present invention has the following beneficial effects: The organoid culture system of the present invention can simulate the in vivo microenvironment: through three-dimensional scaffolds, dynamic fluid shear force and bioactive factors, it can comprehensively simulate the in vivo microenvironment, significantly improving the maturity and functional differentiation of organoids.
[0024] This invention can improve culture efficiency: the microfluidic chip design enables efficient exchange of nutrients and metabolic waste, thereby improving the survival rate and growth rate of organoids.
[0025] The present invention can enhance culture stability: the use of degradable microcarriers avoids excessive cell aggregation and necrosis, while the degradation rate of the scaffold material matches the tissue growth, which is beneficial for long-term culture.
[0026] This invention can improve experimental reproducibility: the scaffold material with well-defined chemical composition and the precisely controlled culture environment reduce batch-to-batch variability and improve the reliability and reproducibility of experimental results.
[0027] The application has wide application prospects: the system is suitable for culture of various kinds of organs, including intestines, liver, kidney and the like, and can be used for drug screening, disease model construction and regenerative medicine research.
[0028] The application solves key problems in existing organoid culture technology through innovative system design and preparation method, and provides powerful technical support for organoid research and application. BRIEF DESCRIPTION OF DRAWINGS
[0029] Figure 1 is a flowchart of the application. DETAILED DESCRIPTION
[0030] The technical solutions in the embodiments of the application will be clearly and completely described below with reference to the drawings in the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the application. EMBODIMENT
[0031] REFERENCE Figure 1 An organoid culture system comprises a biocompatible scaffold, a microfluidic chip, degradable microcarriers, a dynamic culture device and a monitoring module.
[0032] The biocompatible scaffold is composed of degradable polymer materials and bioactive factors, and has a three-dimensional porous structure.
[0033] The microfluidic chip contains multiple culture chambers and microchannels, and can accurately control the flow of culture solution.
[0034] The degradable microcarriers carry stem cells or progenitor cells, and can be gradually degraded during the culture process.
[0035] The dynamic culture device can provide periodic fluid shear force, simulating the in-vivo hemodynamic environment.
[0036] The monitoring module can monitor parameters such as pH value, dissolved oxygen and metabolite concentration in real time.
[0037] The embodiment also discloses a preparation method of the organoid culture system, comprising the following steps: scaffold material synthesis: degradable polymer materials are compounded with bioactive factors to prepare a three-dimensional scaffold.
[0038] Microcarrier preparation: degradable microcarriers loaded with cells are prepared by using an emulsification-crosslinking method.
[0039] Microfluidic chip processing: a microfluidic chip is manufactured by using photolithography and soft lithography technology.
[0040] System assembly: integrating the scaffold, microcarriers, and microfluidic chip into a dynamic culture device.
[0041] Cell seeding and culture: seeding stem cells or progenitor cells onto microcarriers for dynamic culture.
[0042] In the scaffold material synthesis step, electrospinning or 3D printing technology is used.
[0043] In the microfluidic chip processing step, the chip body is made of PDMS material.
[0044] In the dynamic culture step, the flow rate and shear force of the culture medium can be adjusted to simulate the physiological environment of different organs.
[0045] The organoid culture system of the present application can simulate the in vivo microenvironment: through three-dimensional scaffolds, dynamic fluid shear force and bioactive factors, it can fully simulate the in vivo microenvironment, significantly improving the maturity and functional differentiation of organoids.
[0046] The present application can improve the culture efficiency: the microfluidic chip design realizes efficient exchange of nutrients and metabolic waste, improving the survival rate and growth rate of organoids.
[0047] The present application can enhance the stability of the culture: the use of degradable microcarriers avoids excessive cell aggregation and necrosis, and the degradation rate of the scaffold material matches the growth of the tissue, which is beneficial for long-term culture.
[0048] The present application can improve the repeatability of experiments: the chemical composition of the scaffold material is clear and the culture environment is precisely controlled, reducing batch-to-batch differences and improving the reliability and repeatability of experimental results.
[0049] The present application has wide application prospects: the system is suitable for the culture of various organoids, including intestinal, liver, kidney, etc., and can be used for drug screening, disease model construction and regenerative medicine research.
[0050] The present application solves the key problems in existing organoid culture technology through innovative system design and preparation method, providing strong technical support for organoid research and application.
[0051] Although embodiments of the present application have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and alterations can be made without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.
Claims
1. An organoid culture system, characterized by: These include biocompatible scaffolds, microfluidic chips, biodegradable microcarriers, dynamic culture devices, and monitoring modules.
2. The organoid culture system of claim 1, wherein, The biocompatible scaffold is composed of biodegradable polymer materials and bioactive factors, and has a three-dimensional porous structure.
3. The organoid culture system according to claim 1, characterized in that, The microfluidic chip contains multiple culture chambers and microchannels, enabling precise control of the flow of the culture medium.
4. The organoid culture system according to claim 1, characterized in that, The biodegradable microcarriers are loaded with stem cells or progenitor cells and can gradually degrade during culture.
5. The organoid culture system according to claim 1, characterized in that, The dynamic culture device can provide periodic fluid shear force to simulate the hemodynamic environment in vivo.
6. The organoid culture system according to claim 1, characterized in that, The monitoring module can monitor parameters such as pH value, dissolved oxygen and metabolite concentration in real time.
7. A method for preparing an organoid culture system, characterized in that: Includes the following steps: Scaffold material synthesis: Three-dimensional scaffolds are prepared by combining biodegradable polymer materials with bioactive factors; Microcarrier preparation: Cell-loaded biodegradable microcarriers were prepared using an emulsification-crosslinking method; Microfluidic chip fabrication: Microfluidic chips are fabricated using photolithography and soft photolithography techniques; System assembly: Integrating scaffolds, microcarriers, and microfluidic chips into a dynamic culture device; Cell seeding and culture: Stem cells or progenitor cells are seeded onto microcarriers and cultured dynamically.
8. The method for preparing an organoid culture system according to claim 7, characterized in that: The scaffold material synthesis process employs electrospinning or 3D printing technology.
9. The method for preparing an organoid culture system according to claim 7, characterized in that: The microfluidic chip fabrication process uses PDMS material to fabricate the chip body.
10. The method for preparing an organoid culture system according to claim 7, characterized in that: The flow rate and shear force of the culture medium can be adjusted in the dynamic culture step to simulate the physiological environment of different organs.