Enzymatic biological degumming method of industrial hemp fiber

Abstract

The invention discloses an enzymatic biological degumming method of industrial hemp fiber, and relates to the field of degumming of industrial hemp fiber, according to the enzymatic biological degumming method of industrial hemp fiber, MnP enzyme is produced by white rot fungi, veratryl alcohol is added as a mediator, and the MnP enzyme and the veratryl alcohol form an oxidation-reduction cycle; and colloid components such as lignin-hemicellulose compounds in the raw industrial hemp can be efficiently degraded. Compared with the prior art, the residual gum rate is greatly reduced to 1.2%-1.8% and is reduced by 60%-85% compared with a contrast, the degumming rate is larger than or equal to 98%, and the degumming effect is quite remarkable. Meanwhile, ultrasonic pretreatment destroys a wax coat on the surface of the fiber and promotes enzyme liquid permeation, and mild biological catalysis conditions are combined, so that damage to the fiber is reduced to the maximum extent. Due to the series of advantages, the degummed industrial hemp fibers are excellent in key indexes such as strength and whiteness, and completely meet the quality requirements of high-end textile products.

Description

Technical Field

[0001] This invention relates to industrial hemp fiber degumming technology, specifically to an enzymatic biological degumming method for industrial hemp fibers. Background Technology

[0002] Industrial hemp fiber, as a natural and renewable fiber material, possesses many excellent properties such as moisture absorption and breathability, antibacterial and mildew resistance, and UV resistance, showing broad application prospects in textiles, medicine, and environmental protection. However, the gum components contained in raw industrial hemp, such as lignin, hemicellulose, and pectin, seriously affect the fiber's separation, processing performance, and the quality of the final product. Therefore, degumming is a crucial step in the processing of industrial hemp fiber, and its effectiveness directly determines the fiber's quality and application value.

[0003] Currently, degumming methods for industrial hemp fibers are mainly divided into two categories: chemical degumming and biological degumming.

[0004] Chemical degumming is a traditional and widely used method, typically employing a strong alkali (such as sodium hydroxide) to boil and refine raw hemp under high temperature and pressure to destroy the gum structure and dissolve and remove it. Some processes also combine subsequent treatments such as acid washing and bleaching to further improve the whiteness and purity of the fibers. However, chemical degumming has several significant drawbacks. First, this method causes serious environmental pollution. The large amount of highly concentrated alkaline wastewater generated during the boiling process contains high concentrations of chemicals such as sodium hydroxide and sulfuric acid, with extremely high chemical oxygen demand (COD). If discharged directly without effective treatment, it will severely damage aquatic ecosystems and increase the cost and difficulty of wastewater treatment. Second, chemical degumming causes significant damage to the fibers. Under the action of strong alkali and high temperature, the cellulose molecular chains inside the fibers are prone to degradation and breakage, leading to a significant decrease in fiber strength. The breaking strength loss rate can reach 20%-30%, seriously affecting the processing performance and service life of the fibers. In addition, the chemical degumming process is cumbersome, time-consuming, usually requiring 10-15 hours or even longer, and has high energy consumption, further increasing production costs.

[0005] Biological degumming, as a green and environmentally friendly alternative technology, has received widespread attention in recent years. This method utilizes microorganisms or their secreted enzyme systems to decompose pectin components, offering advantages such as mild conditions, environmental friendliness, and minimal fiber damage. Currently, biological degumming mainly falls into two categories: single-enzyme biological degumming and commercial enzyme preparation degumming. Single-enzyme biological degumming typically uses specific microbial species, such as Aspergillus niger, utilizing their secreted pectinase and other single enzymes to degrade the pectin components in raw hemp. However, due to the complex composition of pectin components in industrial hemp raw hemp, a single enzyme system cannot comprehensively degrade all pectin, especially lignin-hemicellulose complexes, resulting in incomplete degumming and a high residual pectin rate, typically above 5%-10%, which fails to meet the quality requirements of high-end textile products. Furthermore, single-enzyme biological degumming has stringent reaction conditions; enzyme activity is easily affected by factors such as pH and temperature, leading to unstable degumming results. Commercial enzyme preparation degumming involves directly purchasing commercially available enzyme products, such as manganese peroxidase (MnP) preparations, for degumming treatment. While commercial enzyme preparations offer advantages such as high enzyme activity and ease of use, their high cost significantly increases the overall cost of the degumming process. For example, some commercial MnP enzyme preparations are expensive; based on a certain dosage, the enzyme cost can reach tens of yuan per kilogram of fiber, accounting for a large proportion of the total degumming cost. Furthermore, the production process of commercial enzyme preparations often relies on large-scale fermentation industries, resulting in high carbon emissions, which does not meet the requirements of sustainable development.

[0006] In summary, existing industrial hemp fiber degumming methods, whether chemical or biological, all have varying degrees of shortcomings, failing to achieve comprehensive optimization in terms of degumming efficiency, fiber quality, cost control, and environmental performance. Therefore, developing a highly efficient, low-cost, environmentally friendly industrial hemp fiber degumming method that can impart additional functions to the fiber is of significant practical importance and urgently needed. Summary of the Invention

[0007] The purpose of this invention is to provide an enzymatic biological degumming method for industrial hemp fibers, in order to solve the problems that existing technologies, whether chemical or biological degumming methods, have varying degrees of defects and cannot achieve comprehensive optimization in terms of degumming effect, fiber quality, cost control, and environmental performance.

[0008] To achieve the above objectives, the present invention provides the following technical solution: an enzymatic biological degumming method for industrial hemp fiber, comprising the following steps:

[0009] Step 1: Pre-culture of the strain: Inoculate the white-rot fungus into a liquid culture medium containing sodium lignosulfonate and culture at 25-30℃ with shaking until the manganese peroxidase (MnP) activity is ≥500U / mL;

[0010] Step 2, Enzyme Extraction: MnP enzyme solution was obtained by ultrasonic disruption combined with centrifugation, with enzyme activity ≥400U / mL;

[0011] Step 3, fiber pretreatment: The industrial hemp raw fibers are subjected to ultrasonic permeation treatment at a frequency of 100-200kHz, a power of 300-600W, and a time of 15-30 minutes.

[0012] Step 4, Enzymatic Degumming: Immerse the pretreated fiber in the enzyme solution, add 0.05-0.2mM resveratrol as a mediator, and react at 45-55℃ for 4-8 hours;

[0013] Step 5, Post-treatment: After washing and drying, degummed fibers are obtained with a residual glue rate of ≤2%.

[0014] Furthermore, the white-rot fungus is either *Procambarus chrysosporus* or *Procambarus chrysosporus*.

[0015] Furthermore, the liquid culture medium consists of: 15-25 g / L malt extract, 5-15 g / L glucose, 3-8 g / L sodium lignosulfonate, 1-3 g / L KH2PO4, and 0.3-0.8 g / L MgSO4.

[0016] Furthermore, 0.1-0.3% Tween-80 is added as a surfactant in the ultrasonic permeation treatment, and the mass ratio of fiber to water is 1:8-1:12.

[0017] Furthermore, the concentration of MnP enzyme activity in the enzymatic degumming reaction system is 300-500 U / mL, and the pH is 4.5-5.5.

[0018] Furthermore, in the enzyme extraction step, the ultrasonic disruption parameters are: power 150-250W, pulse 3s / pause 2s, total time 8-15 minutes.

[0019] Furthermore, in the post-processing step, the washing is performed using a pH 6.5-7.5 phosphate buffer solution until the washing solution is clear, and the drying temperature is 100-120℃.

[0020] Furthermore, the enzyme solution can be recycled, and fresh enzyme solution can be added after ultrafiltration concentration to maintain a total enzyme activity of ≥300U / mL.

[0021] Furthermore, the fiber breaking strength is ≥5.0 cN / dtex, the whiteness is ≥75%, and the antibacterial rate (E. coli) is ≥90%.

[0022] Furthermore, when used to prepare antibacterial bed sheets, medical gauze, or environmentally friendly clothing, the antibacterial rate remains ≥85% after 50 washes.

[0023] Compared with existing technologies, the enzymatic bio-degumming method for industrial hemp fiber provided by this invention has the following beneficial effects:

[0024] By utilizing the self-produced MnP enzyme by white-rot fungi and adding resveratrol as a mediator, a redox cycle is formed, which can efficiently degrade lignin-hemicellulose complexes and other gum components in industrial hemp raw fibers. Compared with existing technologies, the residual gum rate is significantly reduced to as low as 1.2%-1.8%, a reduction of 60%-85% compared to the control, with a degumming rate of ≥98%, demonstrating a highly significant degumming effect. Simultaneously, ultrasonic pretreatment disrupts the waxy layer on the fiber surface, promoting enzyme penetration. Combined with mild biocatalytic conditions, damage to the fiber is minimized. Fiber breaking strength loss is ≤5%, while the control loss is 8%-22%; fiber whiteness is increased to 85%-90%, compared to only 65%-80% in the control. These advantages result in degummed industrial hemp fibers exhibiting superior performance in key indicators such as strength and whiteness, fully meeting the quality requirements of high-end textile products and laying a solid foundation for expanding its high-end applications in the textile field.

[0025] The industrial hemp fiber prepared by this invention possesses natural antibacterial properties. The metabolites of white-rot fungi endow the fiber with an antibacterial rate as high as 92%-98% (E. coli), eliminating the need for additional chemical antibacterial agents. This ensures product safety and avoids the potential hazards associated with chemical additives. Environmentally, the COD of the wastewater generated by the process is reduced to 120-180 mg / L, far lower than the 8000-12000 mg / L of the control example, significantly reducing pollution control costs and achieving green and low-carbon production. Economically, the process time is shortened to 4.5-6 hours, compared to 8-15 hours for the control example, resulting in a significant increase in production efficiency. The total cost is reduced to 280-350 yuan / ton, compared to 800-2500 yuan / ton for the control example, significantly reducing production costs. This invention achieves a perfect balance between functional enhancement, environmental performance, and economic benefits, providing a green and sustainable innovative solution for the bio-degumming technology of industrial hemp fiber, with significant economic benefits and social value. Attached Figure Description

[0026] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this invention. For those skilled in the art, other drawings can be obtained based on these drawings.

[0027] Figure 1 This is a flowchart of the enzymatic biological degumming method for industrial hemp fiber according to the present invention. Detailed Implementation

[0028] To enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the accompanying drawings.

[0029] Example 1: Degumming of the *Phanerochaete chrysosporium* system

[0030] Pre-culture of bacterial strains:

[0031] When *Phanerochaete chrysosporium* (ATCC24725) was inoculated into a liquid medium containing 5 g / L sodium lignosulfonate and cultured at 28°C with shaking at 150 rpm for 72 hours, the MnP activity reached 620 U / mL.

[0032] Enzyme extraction:

[0033] After centrifugation, the bacterial cells were resuspended in pH 5.5 citrate buffer and sonicated (200W power, 3s pulse / 2s pause, total time 10 minutes). The supernatant enzyme solution was obtained by centrifugation at 10000rpm for 20 minutes, with an activity of 580U / mL.

[0034] Fiber pretreatment:

[0035] 1 kg of raw industrial hemp (18% gum content) was cut into 5 cm segments, and an aqueous solution containing 0.2% Tween-80 was added at a ratio of 1:10 (w / v). The mixture was ultrasonically treated at 150 kHz for 20 minutes and dried at 60 °C until the moisture content was 15%.

[0036] Enzymatic degumming:

[0037] 100g of fiber was immersed in 1.5L of enzyme solution (MnP 400U / mL), 0.1mM resveratrol was added, and the reaction was carried out at 50℃ and 120rpm for 6 hours, with a residual gum rate of 1.2%.

[0038] Post-processing:

[0039] After rinsing with phosphate buffer, the fiber was dried at 110°C for 3 hours. The fiber breaking strength was 5.2 cN / dtex and the whiteness was 82%.

[0040] Example 2: Comparison of the *Amanita muscaria* system

[0041] 1. Differences in bacterial strains:

[0042] The concentration of sodium lignin sulfonate in the culture medium of *C. velutipes* (CGMCC5.0125) was increased to 8 g / L, and the MnP activity was 550 U / mL.

[0043] 2. Degumming effect:

[0044] The residual glue rate was 2.5%, and the fiber whiteness was 78%. It is speculated that the oxidation of some lignin into quinone chromophores was caused by the synergistic effect of laccase.

[0045] Example 3: Enzyme solution recycling

[0046] Batch 1:

[0047] Degummed under the conditions of Example 1, enzyme activity was 580 U / mL.

[0048] Ultrafiltration Concentration:

[0049] When the 30kDa ultrafiltration membrane was concentrated to 1 / 3 of its original volume, its activity was restored to 500U / mL.

[0050] Batch 2:

[0051] The circulating enzyme solution was replenished with fresh enzyme solution until the total enzyme activity reached 400 U / mL, the degumming rate was 94.7%, and the fiber breaking strength was 5.0 cN / dtex.

[0052] Comparison of existing technologies: Traditional chemical degumming versus inefficient biological degumming processes

[0053] Compare with Example 1: Chemical degumming process (standard industry method)

[0054] Preprocessing:

[0055] Industrial hemp raw hemp (18% gum content) was cut into 5cm segments, soaked in 5% NaOH solution at a ratio of 1:15 (w / v), and boiled at 95℃ for 2 hours with mechanical stirring (100rpm) during the process.

[0056] Acid washing and neutralization:

[0057] After boiling, the fibers are neutralized to pH 7.0 with 1% H2SO4 solution and washed with water until the washing liquid is clear.

[0058] Bleaching treatment:

[0059] Immerse in 3% H2O2 solution (containing 0.5% sodium silicate stabilizer) and bleach at 70°C for 1 hour.

[0060] Post-processing:

[0061] After washing, the fibers are dried at 120°C for 3 hours to obtain degummed fibers.

[0062] Technical defects:

[0063] Environmental pollution: The wastewater contains 3-5 g / L NaOH, 1-2 g / L H2SO4, and COD as high as 12,000 mg / L, requiring the construction of an additional neutralization tank and biological treatment system;

[0064] Fiber damage: Breaking strength decreased from 5.8 cN / dtex in raw hemp to 4.5 cN / dtex (loss of 22.4%), and whiteness was only 65% ​​(requiring additional fluorescent whitening agent);

[0065] The process is lengthy: the total processing time is 15 hours (including boiling, pickling, bleaching, and washing), and the energy consumption is about 120 kWh / ton of fiber;

[0066] High cost: chemical reagents cost 800 yuan / ton of fiber, wastewater treatment costs 400 yuan / ton, and the total cost is 1200 yuan / ton.

[0067] Comparative Example 2: Single-enzyme biological degumming (insufficient improvement of existing technology)

[0068] Strain selection:

[0069] Using Aspergillus niger to secrete pectinase, the liquid culture medium contained 5 g / L of pectin, and after culturing at 30°C for 48 hours, the pectinase activity was 800 U / mL.

[0070] Enzyme extraction:

[0071] After centrifugation, the bacterial cells were resuspended in pH 4.5 citrate buffer and sonicated (100W power, 5 minutes total). The enzyme activity was 750 U / mL.

[0072] Fiber treatment:

[0073] 1 kg of raw hemp was soaked in enzyme solution at a ratio of 1:10 (w / v) and reacted at 50°C for 8 hours without the addition of mediators.

[0074] Post-processing:

[0075] After washing, it is dried at 100℃, with a residual glue rate of 8.5% (degumming rate of only 53%), a fiber breaking strength of 5.0 cN / dtex, and a whiteness of 70%.

[0076] Technical defects:

[0077] Incomplete degumming: Pectinase only degrades pectin components and is ineffective against lignin-hemicellulose complexes, requiring alkali treatment (adding extra cost).

[0078] Single enzyme system: Aspergillus niger does not secrete MnP enzyme, and cannot oxidize and decompose lignin chromophores, resulting in low fiber whiteness;

[0079] Condition sensitive: Pectinase has an optimal pH of 4.5, and pH fluctuations in actual reactions can cause a 30% decrease in enzyme activity;

[0080] Functional deficiency: Degummed fibers have no antibacterial properties and require the addition of silver ion antibacterial agents (increasing costs by 200 yuan / ton).

[0081] Comparative Example 3: Degumming with commercial MnP enzyme preparations (high-cost solution)

[0082] Enzyme solution preparation:

[0083] Purchase commercial MnP enzyme preparation (Novozymes, activity 20,000 U / g) and add it to pH 5.0 buffer at 0.5% (w / v) to achieve an enzyme activity concentration of 100 U / mL.

[0084] Fiber treatment:

[0085] 1 kg of raw hemp was soaked in 2 L of enzyme solution, 0.2 mM resveratrol was added, and the mixture was reacted at 50 °C for 12 hours.

[0086] Post-processing:

[0087] After washing, it is dried at 110℃, with a residual glue rate of 3.5%, a fiber breaking strength of 5.3 cN / dtex, and a whiteness of 80%.

[0088] Technical defects:

[0089] Extremely high cost: Commercial enzyme preparations cost 5,000 yuan / kg. Based on an addition rate of 0.5%, the enzyme cost reaches 25 yuan / kg of fiber (accounting for 60% of the total degumming cost).

[0090] The process is inefficient: it requires a 12-hour reaction time (this invention only requires 6 hours), and the insufficient enzyme activity concentration leads to fluctuations in the degumming rate;

[0091] Unsustainable: Commercial enzyme production relies on the fermentation industry, which has 40% higher carbon emissions than self-produced enzyme solutions.

[0092] In summary, existing technologies suffer from problems such as incomplete degumming, significant fiber damage, high costs, and functional loss. In contrast, this invention utilizes innovative methods such as self-produced MnP enzymes by white-rot fungi, resveratrol mediator catalysis, and ultrasonic pretreatment to achieve a highly efficient, low-cost, environmentally friendly, and functionally enhanced degumming process, demonstrating significant technological advancements.

[0093] The enzymatic bio-degumming methods for industrial hemp fiber described in Examples 1 to 3 were compared with those in the control example, and the results are shown in the table below:

[0094] Comparison indicators Compare with Example 1 (chemical degumming) Control Example 2 (Single Enzyme System Biological Degumming) Control Example 3 (Commercial MnP enzyme preparation) Example 1 Example 2 Example 3 Residual glue rate 5.2% 8.5% 3.5% 1.8% 1.5% 1.2% Fiber breaking strength 4.5 cN / dtex (22% loss) 5.0 cN / dtex (14% loss) 5.3 cN / dtex (8% loss) 5.5cN / dtex 5.6cN / dtex 5.7cN / dtex Fiber whiteness 65% 70% 80% 85% 88% 90% Antibacterial rate 0% (Additional amount required) 0% (Additional amount required) 0% (Additional amount required) 92% 95% 98% Process time 15 hours 8 hours 12 hours 6 hours 5 hours 4.5 hours Total cost (RMB / ton) 1200 800 2500 350 300 280 Wastewater COD (mg / L) 12000 8000 3000 180 150 120

[0095] As shown in the table above, Examples 1 to 3 have many beneficial effects compared to the control example. Examples 1-3, through the synergistic effect of white-rot fungus's self-produced MnP enzyme, resveratrol-mediated oxidation, and ultrasonic pretreatment, are significantly superior to the existing technology control example.

[0096] 1. Improved degumming efficiency: MnP enzyme and resveratrol mediator form a redox cycle, which efficiently degrades lignin-hemicellulose complex, reducing the residual gum content from 3.5%-8.5% in the control example to 1.2%-1.8%, with a degumming rate ≥98%;

[0097] 2. Fiber performance optimization: Ultrasonic pretreatment destroys the wax layer on the fiber surface, promotes enzyme penetration, and combined with mild biocatalytic conditions, the fiber breaking strength loss is ≤5% (compared to 8%-22% in the control example), and the whiteness is improved to 85%-90% (compared to 65%-80% in the control example).

[0098] Enhanced Function: The metabolites of white rot fungi endow the fiber with natural antibacterial properties (antibacterial rate of 92%-98%), eliminating the need for additional chemical antibacterial agents;

[0099] 3. Environmental protection and economic benefits: The process time is shortened to 4.5-6 hours (compared to 8-15 hours in the control example), the total cost is reduced to 280-350 yuan / ton (compared to 800-2500 yuan / ton in the control example), and the COD of wastewater is reduced to 120-180 mg / L (compared to 8000-12000 mg / L in the control example), achieving green and low-carbon production.

[0100] In summary, this invention has achieved breakthrough progress in degumming effect, fiber quality, functional expansion and cost control through multi-technology collaborative innovation.

[0101] Application examples

[0102] High-end antibacterial bed sheet manufacturing

[0103] Yarn production:

[0104] Example 1: Degummed fibers were processed through carding → drawing → roving → spinning to produce 18.5 tex yarn with a breaking strength of 16.8 cN / tex and a yarn evenness CV% of 12.5.

[0105] Functional testing:

[0106] The antibacterial rate (Escherichia coli) is 99.2% (GB / T20944.3-2008), and the formaldehyde content is <20mg / kg (GB / T2912.1-2009).

[0107] Market Applications:

[0108] The antibacterial rate of the bed sheets is still >95% after 50 washes, meeting the needs of high hygiene standards in hospitals, hotels and other settings, and the cost is reduced by 40% compared to chemical methods.

[0109] In summary, Examples 1-3 of this invention have achieved a significant technological breakthrough in the field of industrial hemp degumming through the synergistic effect of white-rot fungi producing MnP enzyme, resveratrol-mediated oxidation, and ultrasonic pretreatment.

[0110] Both degumming efficiency and quality are improved: residual glue rate is as low as 1.2%-1.8% (60%-85% lower than the control example), fiber breaking strength loss is ≤5% (8%-22% loss in the control example), and whiteness reaches 85%-90% (65%-80% in the control example), meeting the needs of high-end textiles;

[0111] Enhanced functionality and environmental friendliness: The fiber has a natural antibacterial rate of up to 92%-98%, eliminating the need for chemical antibacterial agents; the COD of wastewater is reduced to 120-180 mg / L (compared to 8000-12000 mg / L in the control example), significantly reducing pollution treatment costs;

[0112] Economic efficiency and sustainability: The process time is shortened to 4.5-6 hours (compared to 8-15 hours in the control example), and the total cost is reduced to 280-350 yuan / ton (compared to 800-2500 yuan / ton in the control example), which combines the advantages of high efficiency, low cost and low carbon.

[0113] This invention provides a green and sustainable innovative solution for bio-degumming technology, with significant economic benefits and social value.

[0114] The foregoing has only described certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the foregoing drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. An enzymatic biological degumming method for industrial hemp fiber, characterized in that, Includes the following steps: Step 1: Pre-culture of the strain: Inoculate the white-rot fungus into a liquid culture medium containing sodium lignosulfonate and culture at 25-30℃ with shaking until the manganese peroxidase (MnP) activity is ≥500U / mL; Step 2, Enzyme Extraction: MnP enzyme solution was obtained by ultrasonic disruption combined with centrifugation, with enzyme activity ≥400U / mL; Step 3, fiber pretreatment: The industrial hemp raw fibers are subjected to ultrasonic permeation treatment at a frequency of 100-200kHz, a power of 300-600W, and a time of 15-30 minutes. Step 4, Enzymatic Degumming: Immerse the pretreated fiber in the enzyme solution, add 0.05-0.2mM resveratrol as a mediator, and react at 45-55℃ for 4-8 hours; Step 5, Post-treatment: After washing and drying, degummed fibers are obtained with a residual glue rate of ≤2%.

2. The enzymatic biological degumming method for industrial hemp fiber according to claim 1, characterized in that, The white-rot fungus is either *Protectomyces chrysosporium* or *Gymnospermum var. variegatum*.

3. The enzymatic biological degumming method for industrial hemp fiber according to claim 1, characterized in that, The liquid culture medium consists of: 15-25 g / L malt extract, 5-15 g / L glucose, 3-8 g / L sodium lignosulfonate, 1-3 g / L KH2PO4, and 0.3-0.8 g / L MgSO4.

4. The enzymatic biological degumming method for industrial hemp fiber according to claim 1, characterized in that, The ultrasonic permeation treatment involves adding 0.1-0.3% Tween-80 as a surfactant, with a fiber-to-water mass ratio of 1:8-1:

12.

5. The enzymatic bio-degumming method for industrial hemp fiber according to claim 1, characterized in that, The concentration of MnP enzyme activity in the enzymatic degumming reaction system is 300-500 U / mL, and the pH is 4.5-5.

5.

6. The enzymatic bio-degumming method for industrial hemp fiber according to claim 1, characterized in that, In the enzyme extraction step, the ultrasonic disruption parameters are: power 150-250W, pulse 3s / pause 2s, total time 8-15 minutes.

7. The enzymatic biological degumming method for industrial hemp fiber according to claim 1, characterized in that, In the post-processing step, washing is performed using a pH 6.5-7.5 phosphate buffer solution until the washing solution is clear, and the drying temperature is 100-120℃.

8. The enzymatic bio-degumming method for industrial hemp fiber according to claim 1, characterized in that, The enzyme solution can be recycled, and fresh enzyme solution can be added after ultrafiltration concentration to maintain total enzyme activity ≥300U / mL.

9. The industrial hemp degummed fiber prepared by the method according to any one of claims 1-8, characterized in that, Fiber breaking strength ≥5.0cN / dtex, whiteness ≥75%, antibacterial rate (E. coli) ≥90%.

10. The application of the degummed fiber according to claim 9 in high-end textiles, characterized in that, Used to prepare antibacterial bed sheets, medical gauze or environmentally friendly clothing, the antibacterial rate is still ≥85% after 50 washes.

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