Device for researching allelopathic effect between plants

Abstract

The invention relates to the technical field of plant allelopathy, and discloses a device for researching allelopathy among plants, which comprises an incubator, and the top of the incubator is connected with a communication bin; and the communication bin is internally provided with a communication structure for performing allelopathic communication between the two observation rooms. According to the invention, the allelochemicals in the current growth stage of the plant in one observation room needing to be collected are correspondingly opened, a ball valve of an air pipe without a branch pipe in the observation room needing to collect the allelochemicals is opened, and then a branch pipe three-way valve installed in the other observation room is opened, so that air flow can only flow into an air bag; then the motor is started to drive the fan to rotate to extract allelochemicals in the observation room to the inner side of the air bag, air flows through the filter disc when the allelochemicals in the observation room are collected through the air bag, and the allelochemicals in the air flows are adsorbed through an elution layer on the surface of the filter disc; the beneficial effect of collecting the allelochemicals released into the air by the plants in real time in the whole growth period or different growth stages of the plants is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of plant allelopathy, in particular to a device for studying the allelopathy between plants. BACKGROUND

[0002] During the growth of plants, plants can transmit information to surrounding plants through allelopathy to affect the growth and development, photosynthesis, respiration, endogenous hormone level, content of metabolic substances in the body and response of plants to adversity of surrounding plants.

[0003] At present, in the research of plant allelopathy, the plant secondary metabolites are usually extracted after grinding the plant materials, and the secondary metabolites are used as allelochemicals to carry out the research of allelopathy. For example, the disclosure of CN120283791A specification 0018 discloses that the biological bacteriostatic agent includes bean root exudates, and the concentration of the bean root exudates is 66.67-142.86 g / L. As a specific embodiment, the concentration of the bean root exudates is calculated as follows: 100 g of beans are soaked, germinated and watered, and the roots of the beans reach a length of 5-7 cm. The beans with roots of 5-7 cm are placed in 1500 mL of high-temperature sterilized deionized water for 24 hours to obtain bean root exudates, which are defined as an initial concentration of 66.67 g / L. As another specific embodiment, 100 g of beans are soaked, germinated and watered, and the roots of the beans reach a length of 5-7 cm. The beans with roots of 5-7 cm are placed in 700 mL of high-temperature sterilized deionized water for 24 hours to obtain bean root exudates, which are defined as an initial concentration of 142.86 g / L. As a specific embodiment, the length of the roots of the beans can be any one of 5 cm, 6 cm or 7 cm.

[0004] Although the allelochemicals of plants are some plant secondary metabolites with small molecular weight and simple structure, the composition and proportion of the allelochemicals produced by plants are different under different growth conditions, different growth stages and different adversity stresses, so that the chemical signals released by plants change. Therefore, it is difficult to truly reflect the allelopathy between plants under different adversity conditions by using the secondary metabolites extracted from plants to study the allelopathy. SUMMARY

[0005] Technical problem solved: In view of the deficiencies of the prior art, the present application provides a device for studying the allelopathy between plants, which has the advantages of collecting allelochemicals released into the air by plants in real time during the entire growth period or different growth stages of plants, releasing the collected allelochemicals into the air, and observing the allelopathy of plants, thereby solving the above technical problems.

[0006] Technical scheme: To achieve the above object, the present application provides the following technical scheme: a device for studying the allelopathy effect between plants, comprising a culture box, a partition plate vertically installed in the middle of the inner side of the culture box, the partition plate being sealed with the four sides of the culture box, the inner side of the culture box being separated by the partition plate into two observation spaces for placing plants, one hinged door being installed in each of the two observation spaces, the hinged doors being located on the front wall of the culture box, two air holes being respectively formed in the top of each of the two observation spaces, each of the air holes being at least two, the air holes being open at the top and located on the top surface of the culture box and being connected to air pipes through flanges, a ball valve being movably installed at the bottom of the air pipes, the air pipes extending to the top of the culture box at the top and being connected to an alternating chamber at the tail end; The alternating chamber is internally installed with an alternating structure for allelopathy communication between the two observation spaces. A fan is further included, the fan being located in the middle of the inner side of the alternating chamber, the fan being located on the left and right sides of the two observation spaces, two air pipes being respectively connected to the front and rear of the fan and extending from the two observation spaces. An extension pipe is further included, the extension pipe being installed at the tail end of the air pipe and being inserted into the inner side of the alternating chamber, the tail end of at least one of the two air pipes extending from each of the observation spaces being provided with the extension pipe, a three-way valve being installed at the middle of the extension pipe, the two ends of the three-way valve being connected to the extension pipes and the last end being connected to an air bag.

[0007] Preferably, the culture box is made of tempered glass or acrylic plate and is bidirectional transparent and is bonded by glass cement.

[0008] Preferably, the three-way valve connected to one end of the air bag further comprises a branch pipe, the branch pipe being a hollow pipe, the tail end of the branch pipe being fixedly connected to a screw sleeve, the screw sleeve also being hollow and being sealingly connected to the tail end of the branch pipe, the tail end of the screw sleeve being threadedly connected to a screw ring fixedly installed on the front wall of the air bag, the screw ring also being hollow and being fixedly connected to an opening in the front wall of the air bag at the tail end, the outer surface of the screw ring being threadedly connected to the inner side of the screw sleeve, and a filter disc being inserted into the inner side of the screw ring.

[0009] Preferably, the surface of the filter disc is further coated with an elution layer, the elution layer being one of Tenax-TA, Tenax-GR, activated carbon and silica gel, and the thickness of the elution layer being 0.1 mm.

[0010] Preferably, the shaft of the fan is further fixedly connected to the output end of an electric motor, the electric motor being installed on the outer surface of the alternating chamber.

[0011] Preferably, the alternating chamber is an internal hollow cylinder perpendicular to the top surface of the culture box, the top and bottom surfaces of the alternating chamber being sealingly connected to the four air pipes extending from the two observation spaces.

[0012] Preferably, the alternating structure further comprises four air pipes extending from the two observation rooms to form a support for the alternating chamber, one of the two air pipes extending from each observation room is connected to the bottom of the alternating chamber to form a vertical shape, and the other is connected to the top of the alternating chamber to form an inverted "L" shape, and the four air pipes and the alternating chamber form an M-shaped support for the alternating chamber.

[0013] Preferably, the alternating chamber is an internal hollow cylinder parallel to the top of the incubator, and the front end and the rear end of the two sides of the alternating chamber are also sealingly connected to the four air pipes extending from the two observation rooms, and the tail ends of the four air pipes are connected to extension pipes, but only one of the two air pipes extending from each observation room has an air bag installed at the tail end of the extension pipe.

[0014] Preferably, the alternating structure further comprises a support bracket, the top of the support bracket is an arc that fits the bottom of the alternating chamber, and the bottom of the support bracket is connected vertically to the top of the alternating chamber.

[0015] Preferably, the fan is located at the center of the inside of the alternating chamber parallel to the top of the incubator, and each of the two ends of the front side and the two ends of the rear side of the fan is opposite to an extension pipe located at the opening of the inside of the alternating chamber.

[0016] Compared with the prior art, the present application provides a device for studying the allelopathic effect between plants, which has the following beneficial effects: 1、The present application closes all the ball valves of the four air pipes to lock the air pipes, and then opens the ball valve of the air pipe without a branch pipe in the observation room corresponding to the current growth stage of the plant whose allelochemicals need to be collected, and then opens the three-way valve with a branch pipe in the other observation room to make the airflow direction only flow into the air bag, at this time the front and rear ends of the branch pipe are indirectly connected to an observation room and an air bag respectively, and then the motor is started to drive the fan to rotate, it is worth noting that the direction of the fan rotation must ensure that the rear end is connected to the air pipe without a branch pipe, at this time the fan forms a pump-like effect between the observation room and the air bag, and the allelochemicals in the observation room are extracted into the inside of the air bag, when the air flow passes through the filter sheet during the collection of the allelochemicals in the observation room, the filter sheet surface uses the elution layer to adsorb the allelochemicals in the air flow, the collected air bag is selected to be disassembled or injected into another observation room, if selected to be disassembled, the air bag is rotated in the opposite direction to make the screw ring and the screw sleeve move relative to each other, so that the screw ring is separated from the screw sleeve, at this time the filter sheet inside the screw ring is exposed, the filter sheet at this time adsorbs the allelochemicals in the observation room, and can be directly taken off for the next step of research, achieving the beneficial effect of real-time collection of allelochemicals released into the air by plants during the entire growth period or different growth stages of plants.

[0017] 2. This invention involves removing the filter and closing all the ball valves of the four air tubes to lock them. Then, to collect allelochemicals from the plant at the current growth stage in a particular observation room, the ball valve of the air tube without a branch pipe in that observation room is opened. Next, the three-way valve of the air tube with a branch pipe in another observation room is opened, ensuring airflow only into the airbag. The front and rear ends of the branch pipe are indirectly connected to one observation room and one airbag, respectively. The motor is then started to drive the fan. It is important to note that the fan's rotation direction must ensure that the rear end connects to the air tube without a branch pipe. The fan creates a pump-like effect between the observation room and the airbag, drawing the allelochemicals from that observation room into the airbag. The ball valve of the air tube without a branch pipe in the first observation room is then closed, and the ball valve of the air tube without a branch pipe in the second observation room is opened. The allelochemicals collected in the first observation room then enter the second observation room, achieving the beneficial effect of releasing the collected allelochemicals into the air and observing the allelochemical effects of plants. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of Embodiment 1 of the present invention; Figure 2 This is a schematic cross-sectional view of the overall structure of Embodiment 1 of the present invention; Figure 3 This is a schematic cross-sectional view of the AC compartment structure according to Embodiment 1 of the present invention; Figure 4 This is a schematic cross-sectional view of the branch pipe structure of the present invention; Figure 5 This is a schematic diagram of the overall structure of Embodiment 2 of the present invention; Figure 6 This is a schematic cross-sectional view of the overall structure of Embodiment 2 of the present invention; Figure 7 This is a schematic cross-sectional view of the AC compartment structure according to Embodiment 2 of the present invention; Figure 8 This is a cross-sectional view of the trachea structure in Embodiment 2 of the present invention.

[0019] The components include: 1. Incubator; 101. Isolation plate; 2. Air vent; 3. Air tube; 301. Ball valve; 4. Exchange chamber; 401. Fan; 5. Extension tube; 501. Three-way valve; 502. Branch tube; 503. Screw sleeve; 6. Airbag; 601. Threaded ring; 602. Filter plate; 7. Support bracket. Detailed Implementation

[0020] With reference to the drawings of the embodiments of the present application, the technical solutions in the embodiments of the present application will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments of the present application, all the other embodiments obtained by a person of ordinary skill in the art without creative effort belong to the scope of protection of the present application.

[0021] Please refer to Figures 1-8 A device for studying the allelopathic effect between plants, comprising a culture box 1, a vertical isolation plate 101 is installed in the middle of the inner side of the culture box 1, the isolation plate 101 forms a seal with the four sides of the culture box 1, and the inner side of the culture box 1 is separated into two observation rooms for placing plants by the isolation plate 101, a hinge door is installed in each of the two observation rooms, and the hinge door is located on the front wall of the culture box, a gas hole 2 is formed in the top of each of the two observation rooms, and the gas hole 2 penetrates the top surface of the culture box 1, the top of the gas hole 2 is open and located on the top surface of the culture box 1, and a gas pipe 3 is connected to the top surface of the culture box 1 through a flange, a ball valve 301 is movably installed at the bottom of the gas pipe 3, and the gas pipe 3 extends to the top of the culture box 1 at the top and is connected to an alternating warehouse 4 at the tail end; The alternating warehouse 4 is respectively corresponding to the two observation rooms on both sides, and an alternating structure for allelopathic communication between the two observation rooms is installed in the alternating warehouse 4; The alternating structure comprises a fan 401, and two gas pipes 3 extended from the two observation rooms are respectively connected to the front and rear of the left and right sides of the fan 401; Further comprising an extension pipe 5, the extension pipe 5 is installed at the tail end of the gas pipe 3 and inserted into the inner side of the alternating warehouse 4, at least one gas pipe 3 extended from each of the two observation rooms is provided with an extension pipe 5 at the tail end, a three-way valve 501 is installed in the middle of the extension pipe 5, and the three-way valve 501 is connected to the extension pipe 5 at both ends and connected to an air bag 6 at the last end.

[0022] The hinge door is the most basic function of the culture box 1 in the laboratory, and is the most common facility for a person skilled in the art, which is used to open the culture box 1. Even on the market, it can be directly purchased, such as the goods shown in the goods number 10195563703492, so it will not be introduced too much.

[0023] Further, the culture box 1 is made of tempered glass or acrylic plate, and is bidirectional transparent, and is bonded by glass cement.

[0024] The culture box 1 is made of tempered glass or acrylic plate, which has a perspective effect due to its transparent characteristics, so as to facilitate data recording by taking pictures or recording screen. The glass glue is a silicone sealant, which is cured like rubber and can stretch with the thermal expansion and contraction of the glass without cracking. Since the adhesion of glass glue to tempered glass or acrylic plate is a very common application in the art, and the application does not improve it, the adhesion step of glass glue is not described again.

[0025] Further, the branch pipe 502 connected to one end of the air bag 6 also includes a branch pipe 502, which is a hollow pipe on the inside. The tail end of the branch pipe 502 is fixedly connected to a screw sleeve 503, which is also hollow and has a tail end that is sealingly connected to the tail end of the branch pipe 502. The tail end of the screw sleeve 503 is threadedly connected and fixedly installed on the screw ring 601 on the front wall of the air bag 6. The inside of the screw ring 601 is also hollow, and the tail end of the screw ring 601 is fixedly connected to the opening on the front wall of the air bag 6. The outer surface of the screw ring 601 is threadedly connected to the inside of the screw sleeve 503, and the inside of the screw ring 601 is inserted with a filter 602.

[0026] The air bag 6 is a rubber air bag, which is a commodity that can be purchased directly on an online shopping platform, with a commodity number of 10086338841781. The air bag 6 can be replaced with different sizes according to the experimental purpose, but the disassembly method is consistent, which is fixed by screwing the screw sleeve 503 and the screw ring 601.

[0027] The air bag 6 connected to the tail end of the branch pipe 502 is used to collect the allelochemicals between the two observation rooms, so that the device can not only exchange allelochemicals but also collect them. Specifically, all ball valves 301 of the four air pipes 3 are closed and locked, and then the ball valves 301 of the air pipes 3 without the branch pipe 502 in the observation room whose allelochemicals need to be collected are opened, and then the ball valve 301 of the air pipe 3 with the branch pipe 502 in the other observation room is opened, so that the airflow direction can only flow into the air bag 6. At this time, the branch pipe 502 is indirectly connected to one observation room and one air bag 6 at the front and rear ends, respectively. Then start the motor to drive the fan 401 to rotate. It is worth noting that the direction of fan rotation must ensure that the rear end is connected to the air pipe 3 without the branch pipe 502. At this time, the fan 401 forms a pump-like effect between the observation room and the air bag 6, which extracts the allelochemicals in the observation room into the inside of the air bag 6. It is worth noting that the air bag 6 extracts allelochemicals in a cross manner, that is, the allelochemicals in the left observation room are extracted, and the three-way valve 501 with the branch pipe 502 on the upper side of the right observation room is opened.

[0028] Further, the surface of the filter 602 is also coated with an elution layer, which is one of Tenax-TA, Tenax-GR, activated carbon and silica gel, and the thickness of the elution layer is 0.1 mm.

[0029] Tenax-TA and Tenax-GR are heat resistant (≤300℃), hydrophobic, and have strong adsorption capacity for C6-C20 allelochemical VOCs such as terpenes and phenols. They are also easily thermally desorbed and suitable for gas chromatography (GC) analysis. Activated carbon has a wide adsorption range, from low-boiling-point to high-boiling-point VOCs, and is low in cost, but it is easily decomposed by thermal desorption. It is used to enrich low-concentration, difficult-to-adsorb allelochemical gases such as small molecule aldehydes. Silica gel is used to adapt to polar allelochemical gases such as alcohols and ketones. When collecting allelochemical substances in the observation room, the gas flow passes through filter 602 via the gasbag 6, and the allelochemical substances in the gas flow are adsorbed by the eluent layer on the surface of filter 602.

[0030] Furthermore, the shaft of fan 401 is also fixedly connected to the output end of a motor, and the motor is mounted on the outer surface of AC compartment 4.

[0031] The motor is a 12V 4000 RPM model with the existing model number WS-555R. Its encoder is a magnetic encoder, which is a micro motor capable of forward and reverse rotation. Since it is a device that can be purchased directly on shopping platforms, the specific operation and installation methods are already publicly available. Furthermore, this application has not made any improvements to the electrodes, but only uses them as a power source to directly connect to the shaft of the fan for driving. Therefore, it will not be described in detail.

[0032] Furthermore, the exchange chamber 4 is an internally hollow cylinder perpendicular to the top surface of the incubator 1, and the top and bottom surfaces on both sides of the exchange chamber 4 are also sealed and connected to four air tubes 3 extending from the two observation rooms.

[0033] As per the instruction manual Figures 2-3 The two observation rooms are interconnected by four air pipes 3 extending from the top and bottom surfaces of the two sides of the exchange chamber 4, which are sealed and connected respectively. In conjunction with the aforementioned technical solution, a ball valve 301 is installed at the bottom of each air pipe 3. This allows for various combinations: if all four ball valves 301 are closed, the two observation rooms become completely independent and do not interfere with each other; if all four ball valves 301 are open, the two air pipes 3 in each observation room complete the chemiluminescent exchange of gases through the exchange chamber 4. Furthermore, by sequentially closing one air pipe 3 on each side of the top and bottom surfaces of the exchange chamber 4, the remaining air pipes 3 in the two observation rooms are positioned such that one is on the top surface of the exchange chamber 4 and the other on the bottom surface, thus creating interconnection. This is coordinated with the motor for forward and reverse rotation. If the air pipe 3 on the left side of the exchange chamber is on its top surface and the air pipe 3 on the right side is on its bottom surface, then the forward fan 401 rotates as shown in the instruction manual. Figure 2As shown, the fan 401 compresses the rear gas, that is, extracts the gas of the left gas pipe 3, and the corresponding gas of the left observation room enters the inside of the alternating warehouse 4. The space in front of the fan, that is, the right gas pipe 3, is output by the fan 401, and the gas of the left observation room enters the right observation room, so as to complete the exchange of the chemical substances. Further, if the gas in the right observation room is introduced into the left observation room, the motor drives the fan 401 to reverse or sequentially closes each one of the gas pipes 3 on the top and bottom surfaces of the alternating warehouse 4, so that the gas pipe 3 on the left side of the alternating warehouse is on the bottom surface, and the gas pipe 3 on the right side is on the top surface. Continue to drive the fan 401 to rotate in the forward direction.

[0034] Further, the alternating structure further includes four gas pipes 3 extending from the two observation rooms to form a support for the alternating warehouse 4. Two gas pipes 3 extending from a single observation room are connected to the bottom surface of the alternating warehouse 4 to form a vertical shape, and the other gas pipe 3 is connected to the top surface of the alternating warehouse 4 to form an inverted "L" shape. The four gas pipes 3 and the alternating warehouse 4 form an M-shaped support for the alternating warehouse 4.

[0035] The two gas pipes 3 at the bottom of the alternating warehouse 4 directly contact the top surface of the incubator 1 when extending towards the alternating warehouse 4, thereby forming a support for the alternating warehouse 4 based on the incubator 1.

[0036] Further, the alternating warehouse 4 is an internally hollow cylindrical body parallel to the top surface of the incubator 1. The front end and the rear end of the alternating warehouse 4 are respectively sealed and connected to the four gas pipes 3 extending from the two observation rooms. The tail ends of the four gas pipes 3 are connected to the extension pipes 5, but only one of the two gas pipes 3 extending from each observation room has a gas bag 6 installed at the tail end of the extension pipe 5.

[0037] As shown in the accompanying drawings Figures 6-7 The front end and the rear end of the alternating warehouse 4 are respectively sealed and connected to the four gas pipes 3 extending from the two observation rooms, thereby forming intercommunication between the two observation rooms. In combination with the foregoing technical solutions, each gas pipe 3 is provided with a ball valve 301 at the bottom. In this way, various combinations are formed, such as the complete closing of the four ball valves 301 to completely form an independent state between the two observation rooms without interference between them. For example, the four ball valves 301 are completely opened, and the two gas pipes 3 of each observation room complete the chemical exchange between the gases through the alternating warehouse 4. Further, sequentially closing each one of the gas pipes 3 on the front end and the rear end of the alternating warehouse 4 on both sides forms intercommunication between the two observation rooms. One is on the front end of the alternating warehouse 4, and the other is on the rear end of the alternating warehouse 4. In combination with the forward and reverse rotation of the motor, if the gas pipe 3 on the left side of the alternating warehouse is on the front end, and the gas pipe 3 on the right side is on the rear end, the fan 401 is rotated in the forward direction at this time. Figure 2As shown, the fan 401 compresses the rear gas, i.e. the gas of the right side air pipe 3, and extracts the gas of the right side observation room to the inside of the alternating warehouse 4. The space in front of the fan 401, i.e. the left side air pipe 3, is outputted by the fan 401, and the gas of the right side observation room is brought into the left side observation room, thereby completing the exchange of the chemical sensate. Further, if the gas of the left side observation room is to be introduced into the right side observation room, the motor is reversed to reverse the fan 401, or one of the front and rear air pipes 3 on the left and right sides of the alternating warehouse 4 is closed in sequence, so that the left side air pipe 3 of the alternating warehouse is at the rear end, the right side air pipe 3 is at the front end, and the motor is continued to be rotated to drive the fan 401 to rotate forward.

[0038] Further, the alternating structure further comprises a support bracket 7, the top of the support bracket 7 is an arc shape matching the bottom surface of the alternating warehouse 4, and the bottom of the support bracket 7 is vertically connected to the top surface of the alternating warehouse 4.

[0039] Further, the fan 401 is located at the center inside of the alternating warehouse 4 parallel to the top surface of the incubator 1, and each of the two ends of the front side and the two ends of the rear side of the fan 401 is provided with an extension pipe 5 located at the opening inside the alternating warehouse 4.

[0040] Embodiment one In this embodiment, the incubator 1 is made of tempered glass or acrylic plate, and is bidirectional transparent, and is adhered by glass cement. In this embodiment, the outer surface of the incubator 1 is also sleeved with a reinforcing rib. Since the reinforcing rib sleeved on the surface of the glass box body is a common technical solution and not a technical improvement, it will not be described in detail. In this embodiment, the elution layer on the surface of the filter sheet 602 is Tenax-T or Tenax-GR, and the thickness is 0.1mm. In this embodiment, the three-way valve 501 is in a default closed state.

[0041] As shown in the drawings Figure 1As shown, the front wall of the incubator 1 of the present application is provided with a hinged door, and the plants are taken out or put in by opening the hinged door. After the plants are put into the observation room, the hinged door is closed. In order to ensure the sealing of the subsequent experiment, a polyethylene film can also be used to cover the hinged door. When two plants for allelopathy experiment enter the incubator 1, the external light of the laboratory can be turned on to supplement light for the plants to promote their photosynthesis. Further, with the oxygen produced by the photosynthesis of the plants, this part of the oxygen contains the allelochemicals of the plants. At this time, the collection of allelochemicals can be started. The gas bag 6 connected at the tail end of the branch pipe 502 connected by the three-way valve 501 is used to collect the allelochemicals of the two observation rooms. The ball valves 301 of the gas pipes 3 are all closed and locked. Then, the allelochemicals of the current growth stage of the plants in the observation room which needs to be collected are collected. The ball valves 301 of the gas pipes 3 in the observation room which needs to collect allelochemicals are opened correspondingly. Then, the three-way valve 501 installed in the other observation room is opened, so that the airflow direction can only flow into the gas bag 6. At this time, the branch pipe 502 is indirectly connected to an observation room and a gas bag 6 at the front and rear ends respectively. Then, the motor is started to drive the fan 401 to rotate. It is worth noting that the direction of the fan rotation must ensure that the rear end is connected to the gas pipe 3 without the branch pipe 502. At this time, the fan 401 forms a pump-like effect between the observation room and the gas bag 6, and the allelochemicals in the observation room are extracted into the inside of the gas bag 6. When the allelochemicals in the observation room are collected by the gas bag 6, the airflow passes through the filter sheet 602. The filter sheet 602 on the surface of the filter sheet 602 adsorbs the allelochemicals in the airflow. The collected gas bag 6 is selected to be detached or injected into another observation room. If it is selected to be detached, the gas bag 6 is rotated in the opposite direction to make the screw ring 601 move relative to the screw sleeve 503, so that the screw ring 601 is separated from the screw sleeve 503. At this time, the filter sheet 602 inside the screw ring 601 is exposed. At this time, the filter sheet 602 adsorbs the allelochemicals in the observation room, which can be directly taken off and peeled off for the next step of research. The filter sheet 602 is heated by electrification, the gas containing allelochemicals is released, and then collected separately. The collected gas containing allelochemicals is sent back into the observation room. The detached gas bag 6 is connected to the branch pipe 502 after being deflated. In order to avoid negative pressure in the observation room where the allelochemicals are extracted, the fan 401 is reversed to supplement air when the gas bag 6 leaves the branch pipe 502.

[0042] Further, the embodiment can also release the collected allelochemicals. First, reverse rotation of the air bag 6 causes the screw ring 601 at the front end to move relative to the screw sleeve 503, so that the screw ring 601 is separated from the screw sleeve 503. At this time, the filter 602 inside the screw ring 601 is exposed, and the filter 602 of the air bag 6 is taken out alone. The air bag 6 is reloaded into the branch pipe 502 by repeating the above technical solution. The ball valve 301 of the air pipe 3 is completely closed and locked. Then, the ball valve 301 of the air pipe 3 in the observation room where the allelochemicals of the current growth stage of the plant need to be collected is opened. Then, the three-way valve 501 of the observation room where the branch pipe 502 is installed is opened, so that the airflow direction can only flow into the air bag 6. At this time, the branch pipe 502 is indirectly connected to an observation room at the front end and an air bag 6 at the rear end. Then, the motor is started to drive the fan 401 to rotate. It is worth noting that the direction of the fan rotation must ensure that the rear connection is the air pipe 3 without the branch pipe 502. At this time, the fan 401 forms a pump-like effect between the observation room and the air bag 6, and the allelochemicals in the observation room are extracted into the air bag 6. Then, the ball valve 301 of the air pipe 3 without the branch pipe 502 in the observation room is closed, and the ball valve 301 of the air pipe 3 without the branch pipe 502 in the other observation room is opened. At this time, the allelochemicals of the plant in the original observation room are collected into the other observation room.

[0043] Further, in the experiment, not only the allelochemicals need to be collected, but sometimes the allelochemicals of two observation rooms also need to be mixed to observe the growth of plants. The four air pipes 3 extending from the two observation rooms are connected to the top and bottom surfaces of the exchange bin 4 on both sides to form intercommunication between the two observation rooms. In combination with the above technical solution, the ball valve 301 is installed at the bottom of each air pipe 3. In this way, various combinations are formed, such as complete independence between the two observation rooms when the four ball valves 301 are completely closed, and no interference between each other. When the four ball valves 301 are completely opened, the two air pipes 3 in each observation room complete the allelochemical exchange between the gases through the exchange bin 4. Further, one air pipe 3 on the top surface of the exchange bin 4 and the other air pipe 3 on the bottom surface of the exchange bin 4 are closed in sequence on the top and bottom surfaces of the exchange bin 4 on both sides. In this way, intercommunication is also formed. In combination with the motor for forward and reverse rotation, if the air pipe 3 on the left side of the exchange bin is on the top surface, and the air pipe 3 on the right side is on the bottom surface, the fan 401 is rotated forward at this time. As shown in the description, the air pipe 3 on the left side of the exchange bin is on the bottom surface, and the air pipe 3 on the right side is on the top surface. At this time, the fan 401 is rotated in the reverse direction. Figure 2As shown, the fan 401 compresses the rear gas, i.e. extracts the gas of the left gas pipe 3, and the corresponding gas of the left observation room enters the inside of the alternating warehouse 4. The space in front of the fan, i.e. the right gas pipe 3, is output by the fan 401, and the gas of the left observation room enters the right observation room, thereby completing the exchange of allelochemicals. Further, if the gas in the right observation room is to be introduced into the left observation room, the motor is reversed to reverse the fan 401, or the top and bottom of each gas pipe 3 on both sides of the alternating warehouse 4 is sequentially closed, so that the gas pipe 3 on the left side of the alternating warehouse is on the bottom, and the gas pipe 3 on the right side is on the top. Continue to rotate the motor to drive the fan 401 to rotate in the forward direction, thereby mixing the allelochemicals of the two observation rooms and observing the experiment.

[0044] In use, two plants enter the incubator 1, and the external light of the laboratory can be turned on to supplement the light for the plants to promote photosynthesis. Further, as the plants produce oxygen through photosynthesis, the oxygen contains allelochemicals of the plants. At this time, the collection of allelochemicals can be started. The air bag 6 connected by the branch pipe 502 at the tail end of the three-way valve 501 is used to collect allelochemicals from the two observation rooms. The ball valve 301 of the four gas pipes 3 is closed and locked, and then the allelochemicals of the current growth stage of the plants in the observation room which needs to be collected are collected. The ball valve 301 of the gas pipe 3 without the branch pipe 502 in the observation room which needs to collect allelochemicals is opened, and then the ball valve 301 of the gas pipe 3 with the branch pipe 502 three-way valve 501 in the other observation room is opened, so that the airflow direction can only flow into the air bag 6. At this time, the branch pipe 502 is indirectly connected to an observation room and an air bag 6 at the front and rear ends, respectively. Then start the motor to drive the fan 401 to rotate. It is worth noting that the direction of the fan rotation must ensure that the rear end is connected to the gas pipe 3 without the branch pipe 502. At this time, the fan 401 forms a pump-like effect between the observation room and the air bag 6, and the allelochemicals in the observation room are extracted into the inside of the air bag 6. When the air bag 6 collects the allelochemicals in the observation room, the airflow passes through the filter 602, and the filter 602 on the surface of the filter 602 adsorbs the allelochemicals in the airflow. The collected air bag 6 is selected to be detached or injected into another observation room. If it is selected to be detached, the air bag 6 is rotated in the opposite direction to make the screw ring 601 move relative to the screw sleeve 503, so that the screw ring 601 is separated from the screw sleeve 503. At this time, the filter 602 inside the screw ring 601 is exposed. At this time, the filter 602 adsorbs the allelochemicals in the observation room, which can be directly taken off and peeled off for the next step of research. The removed filter 602 is heated by electrification to release the gas containing allelochemicals, and then the gas containing allelochemicals is collected separately and sent back into the observation room.

[0045] Example Two In the embodiment, the exchange chamber 4 is a hollow cylinder parallel to the top surface of the incubator 1. In the embodiment, the incubator 1 is made of tempered glass or acrylic plate, is bidirectional transparent, and is bonded by glass cement. In the embodiment, the incubator 1 is not shown in the drawings and is further sleeved with a reinforcing rib on the outer surface. Since the reinforcing rib sleeved on the surface of the glass box body is a common technical solution and is not a technical improvement, further description is omitted. In the embodiment, the elution layer on the surface of the filter sheet 602 is Tenax-T or Tenax-GR, and the thickness is 0.1 mm. When two plants that need to be subjected to allelopathy experiment enter the incubator 1, the external light of the laboratory can be turned on to supplement light for the plants to promote photosynthesis. Further, oxygen generated by photosynthesis of the plants contains allelochemicals of the plants. At this time, the allelochemicals can be collected. The four gas pipes 3 extending from the front end and the rear end of the two sides of the exchange chamber 4 are respectively sealed and connected to form intercommunication between the two observation rooms. According to the foregoing technical solution, the ball valve 301 is installed at the bottom of each gas pipe 3. In this way, various combinations are formed. For example, when all the four ball valves 301 are closed, the two observation rooms are completely independent and do not interfere with each other. When all the four ball valves 301 are opened, the two gas pipes 3 in each observation room complete the allelopathic communication between the gases through the exchange chamber 4. Further, one gas pipe 3 on the front end of the exchange chamber 4 and the other gas pipe 3 on the rear end of the exchange chamber 4 are left in the two observation rooms by sequentially closing each one of the gas pipes 3 on the front end and the rear end of the two sides of the exchange chamber 4. In this way, intercommunication is also formed. In cooperation with the motor, if the gas pipe 3 on the left side of the exchange chamber is at the front end and the gas pipe 3 on the right side is at the rear end, the fan 401 is rotated in the forward direction. As shown in the description and the drawings, the fan 401 compresses the gas at the rear end, that is, the gas in the gas pipe 3 on the right side, and the gas in the observation room on the right side enters the inside of the exchange chamber 4. The space in front of the fan 401, that is, the gas pipe 3 on the left side, is output by the fan 401, and the gas in the observation room on the right side enters the observation room on the left side, so that the exchange of allelochemicals is completed. Further, if the gas in the observation room on the left side is introduced into the observation room on the right side, the motor is reversed or each one of the gas pipes 3 on the front end and the rear end of the two sides of the exchange chamber 4 is sequentially closed, so that the gas pipe 3 on the left side of the exchange chamber is at the rear end and the gas pipe 3 on the right side is at the front end. The motor is continuously rotated in the forward direction to drive the fan 401 to rotate in the forward direction. Figure 2

[0046] ​Furthermore, this embodiment can also release the collected allelochemicals. First, rotate the airbag 6 in the opposite direction so that the screw ring 601 at its front end moves relative to the screw sleeve 503, causing the screw ring 601 to disengage from the screw sleeve 503. At this time, the filter 602 inside the screw ring 601 is exposed. The filter 602 of the airbag 6 is removed separately. Repeat the aforementioned technical solution to reinstall the airbag 6 into the branch pipe 502. Close all the ball valves 301 of the four air pipes 3 to lock the air pipes 3. Then, to collect allelochemicals of the current growth stage of the plants in the observation room, open the ball valve 301 of the air pipe 3 in the observation room where the allelochemicals need to be collected that does not have the branch pipe 502 installed. Then open the three-way valve of the other observation room where the branch pipe 502 is installed. 501, ensuring that the airflow direction can only flow into the airbag 6. In embodiment 1, the airflow direction of the exchange chamber 4 is longitudinal, while in this embodiment, the airflow direction is transverse. Start the motor to drive the fan 401 to rotate. It is worth noting that the direction of the fan rotation must ensure that the air pipe 3 connected to the rear is not connected to the branch pipe 502. At this time, the fan 401 forms a pump-like effect between the observation room and the airbag 6, drawing the allelochemicals from the observation room into the inside of the airbag 6. Then, close the ball valve 301 of the air pipe 3 in the observation room that is not connected to the branch pipe 502, and open the ball valve 301 of the air pipe 3 in another observation room that is not connected to the branch pipe 502. At this time, the plant allelochemicals collected in the observation room enter the other observation room.

[0047] Furthermore, in this embodiment, the three-way valve 501 is kept in the normally closed state of the air chamber 6 by default. In experiments, it is not only necessary to collect allelochemicals, but sometimes it is also necessary to mix allelochemicals from two observation rooms to observe plant growth. The four air pipes 3 extending from the two observation rooms are sealed and connected to the front and rear ends of the two sides of the exchange chamber 4, forming an interconnection between the two observation rooms. Combined with the aforementioned technical solution, a ball valve 301 is installed at the bottom of each air pipe 3. This allows for various combinations. For example, if all four ball valves 301 are closed, the two observation rooms become completely independent and do not interfere with each other. Alternatively, if all four ball valves 301 are open, the two air pipes 3 of each observation room complete the allelochemical exchange of gases through the exchange chamber 4. Further, by sequentially closing one air pipe 3 at the front and one at the rear of each side of the exchange chamber 4, the remaining air pipes 3 of the two observation rooms are positioned such that one is at the front end of the exchange chamber 4 and the other at the rear end, thus forming an interconnection. This is coordinated with the motor for forward and reverse rotation. If the air pipe 3 on the left side of the exchange chamber is at its front end and the air pipe 3 on the right side is at its rear end, then the fan 401 rotates forward, as shown in the attached instruction manual. Figure 2As shown, the fan 401 compresses the rear gas, that is, the gas of the right side air pipe 3, and extracts the gas of the right side observation room into the air exchange room 4. The space in front of the fan 401, that is, the left side air pipe 3, is outputted by the fan 401, and the gas of the right side observation room is brought into the left side observation room, so as to complete the exchange of the allelochemicals. Further, if the gas of the left side observation room is to be introduced into the right side observation room, the motor is reversed to reverse the fan 401, or one of the front end and the rear end of the air pipe 3 on the left side of the air exchange room 4 is closed in sequence, so that the air pipe 3 on the left side of the air exchange room is at the rear end, the air pipe 3 on the right side is at the front end, the motor is continuously rotated to drive the fan 401 to rotate in the forward direction, so as to perform the mixing observation experiment of the allelochemicals on the plants in the two observation rooms.

[0048] Although the embodiments of the present application have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. An apparatus for studying allelopathy among plants includes a culture chamber (1), wherein a partition plate (101) is vertically mounted on the middle of the inner side of the culture chamber (1), the partition plate (101) and the four sides of the culture chamber (1) are sealed, and the inner side of the culture chamber (1) is separated by the partition plate (101) into two observation rooms for placing plants, characterized in that: Each of the two observation rooms has an air hole (2) that penetrates the top surface of the incubator (1) on its inner side. Each observation room has at least two air holes (2). The top of the air hole (2) is open and located on the top surface of the incubator (1). An air pipe (3) is connected to the air pipe (3) through a flange. A ball valve (301) is movably installed at the bottom of the air pipe (3). The top of the air pipe (3) extends toward the top of the incubator (1) and the tail end is connected to the communication chamber (4). The communication chamber (4) is equipped with an communication structure for allelopathic communication between the two observation rooms. Includes a fan (401), which is located in the middle of the inner side of the communication chamber (4). The fan (401) has two observation rooms on its left and right sides, and the fan (401) is connected to the air pipes (3) extending from the two observation rooms at the front and rear. It also includes an extension tube (5), which is installed at the end of the trachea (3) and inserted into the inside of the communication chamber (4). At least one of the two tracheas (3) extending from each observation room has an extension tube (5) installed at its end. A three-way valve (501) is installed in the middle of the extension tube (5). The two ends of the three-way valve (501) are respectively connected to the extension tube (5) and the last end is movably connected to the airbag (6).

2. The apparatus for studying allelopathic effects among plants according to claim 2, characterized in that: The fan (401) is also fixedly connected to the output end of a motor, which is mounted on the outer surface of the AC compartment (4).

3. The apparatus for studying allelopathy among plants according to claim 1 or 2, characterized in that: The incubator (1) is made of tempered glass or acrylic sheet, and is transparent in both directions, and is bonded with glass glue.

4. The apparatus for studying allelopathy among plants according to claim 3, characterized in that: The three-way valve (501) is connected to one end of the airbag (6) and also includes a branch pipe (502). The branch pipe (502) is a hollow pipe. The tail end of the branch pipe (502) is fixedly connected to a threaded sleeve (503). The threaded sleeve (503) is also hollow and its tail end forms a sealed connection with the tail end of the branch pipe (502). The tail end of the threaded sleeve (503) is threadedly connected to a threaded ring (601) fixedly installed on the front wall of the airbag (6). The inner side of the threaded ring (601) is also hollow and its tail end is fixedly connected to the opening on the front wall of the airbag (6). The outer surface of the threaded ring (601) is threadedly connected to the inner side of the threaded sleeve (503). A filter (602) is inserted into the inner side of the threaded ring (601).

5. The apparatus for studying allelopathy among plants according to claim 4, characterized in that: The filter (602) surface is also coated with an elution layer, which is one of Tenax-TA, Tenax-GR, activated carbon and silica gel, and the thickness of the elution layer is 0.1 mm.

6. The apparatus for studying allelopathy among plants according to claim 5, characterized in that: The communication chamber (4) is an internal hollow cylinder perpendicular to the top surface of the incubator (1). The top and bottom surfaces on both sides of the communication chamber (4) are also sealed and connected to four air tubes (3) extending from the two observation rooms.

7. The apparatus for studying allelopathy among plants according to claim 6, characterized in that: The communication structure also includes four air pipes (3) extending from the two observation rooms to support the communication chamber (4). One air pipe (3) extending from a single observation room is connected to the bottom surface of the communication chamber (4) to form a vertical shape, and the other air pipe (3) is connected to the top surface of the communication chamber (4) to form an inverted "L" shape. The four air pipes (3) and the communication chamber (4) together form an M-shaped support for the communication chamber (4).

8. The apparatus for studying allelopathy among plants according to claim 4, characterized in that: The communication chamber (4) is an internal hollow cylinder parallel to the top surface of the incubator (1). The front and rear ends of the two sides of the communication chamber (4) are also sealed and connected to four air tubes (3) extending from the two observation rooms. The tail ends of the four air tubes (3) are all connected to extension tubes (5), but only one of the two air tubes (3) extending from each observation room has an air bag (6) installed on the extension tube (5) at the tail end of the air tube (3).

9. The apparatus for studying allelopathy among plants according to claim 8, characterized in that: The communication structure also includes a support bracket (7), the top of which is an arc shape that fits the bottom surface of the communication compartment (4), and the bottom of which is vertically connected to the top surface of the communication compartment (4).

10. The apparatus for studying allelopathy among plants according to claim 9, characterized in that: The fan (401) is located at the center of the inner side of the communication chamber (4) parallel to the top surface of the incubator (1), and each of the front and rear ends of the fan (401) has an extension tube (5) with an opening inside the communication chamber (4).

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