Multi-channel sample detection device and method based on lateral flow test strip

Abstract

The invention discloses a multi-channel sample detection device and method based on lateral flow test strips. The multi-channel sample detection device comprises a sample pad, an upper pressure fixing disc, a sample distribution disc, a hydrophilic film and a test strip distribution disc, the sample distribution disc is provided with a sample distribution area and a sample distribution runner; the sample pad is arranged in the sample distribution area; the hydrophilic film is adhered to the back surfaces of the sample pad and the sample distribution disc; the test strip distribution disc sleeves the periphery of the sample distribution disc, and the test strip distribution disc is a test strip area; and the upper pressing fixed disc is pressed above the periphery of the sample distribution disc and above the test strip distribution disc. A liquid sample flows to the corresponding test strips in the test strip distribution plate through the plurality of sample distribution runners of the sample distribution plate for detection, external power and complex equipment are not needed, and the liquid sample can be detected only through micro-runner optimization, structural design and capillary force effect. The liquid sample to be detected can be quickly, uniformly and automatically distributed in a plurality of detection flow channels, so that the consistency, reliability and operation convenience of a multi-channel detection result are effectively improved.

Description

Technical Field

[0001] This invention relates to the field of testing equipment technology, and specifically to a multi-channel sample testing device and method based on a side-flow test strip. Background Technology

[0002] Sideflow chromatography, due to its speed and convenience, has been widely used in medical diagnostics, food safety testing, and other fields. To achieve multi-indicator detection in a single sample, multi-channel sideflow detection devices have become an important development direction, as integrating multiple detection indicators into a single test strip presents problems such as false positives and complex preparation processes.

[0003] Currently, common multi-channel detection methods have the following main shortcomings: First, manually adding samples to multiple test strips one by one using a pipette is cumbersome and prone to uneven distribution due to human error, affecting the accuracy and comparability of the detection. Second, some integrated microfluidic devices use complex active pumps or centrifuges to drive liquid distribution and delivery. While this improves accuracy, it results in high equipment costs and complex structures, making it difficult to popularize in grassroots or field-based rapid detection scenarios. Furthermore, some passive distribution designs that rely on capillary force often suffer from inconsistent liquid distribution speeds and volumes due to minute differences in hydrophilicity / hydrophobicity between channels or manufacturing errors. This leads to asynchronous reaction times and varying detection signal strengths between channels, ultimately affecting the reliability of the results.

[0004] Therefore, there is an urgent need for a multi-channel detection solution that requires no external power, is easy to operate, and can achieve high-precision automatic liquid separation. Summary of the Invention

[0005] This invention addresses the shortcomings of existing technologies by providing a multi-channel sample detection device and method based on a side-flow test strip. It does not rely on external power or complex equipment, but achieves rapid, uniform, and automatic distribution of the liquid sample to be tested in multiple detection channels through microchannel optimization, structural design, and capillary force alone. This effectively improves the consistency, reliability, and ease of operation of multi-channel detection results.

[0006] To address the aforementioned technical problems, the first aspect of the present invention provides a multi-channel sample detection device based on a side-flow test strip, comprising a sample pad, an upper pressure fixing plate, a sample dispensing plate, a hydrophilic membrane, and a test strip dispensing plate;

[0007] The sample distribution disk is provided with a sample distribution area, and several sample distribution channels are arranged radially outward from the sample distribution area as the center;

[0008] The sample pad is placed in the sample dispensing area, and the hydrophilic membrane is adhered to the back of the sample pad and the sample dispensing tray;

[0009] The test strip distribution disk is fitted around the outer periphery of the sample distribution disk. The test strip distribution disk has a test strip area along the extension direction of the sample distribution channel for placing test strips. The inner end of the test strip is connected to the sample distribution channel.

[0010] The upper pressure fixing plate presses on the outer periphery of the sample distribution plate and the test strip distribution plate.

[0011] In this invention, liquid samples from the sample pad flow through multiple sample distribution channels in the sample distribution tray to the corresponding test strips in the test strip distribution tray for detection. This eliminates the need for external power and complex equipment. Through microchannel optimization, structural design, and capillary force, it achieves rapid, uniform, and automatic distribution of the liquid sample across multiple detection channels, effectively improving the consistency, reliability, and ease of operation of multi-channel detection results. Furthermore, the number of sample distribution channels and their corresponding test strips can be adjusted according to actual needs, offering high adaptability.

[0012] Furthermore, the sample dispensing tray is made of cellulose paper, and the sample dispensing channels of the sample dispensing tray are formed by wax printing.

[0013] Furthermore, a first sample buffer zone and a second sample buffer zone are provided on the sample dispensing channel. The first sample buffer zone is pre-filled with a soluble reagent stopper, and the second sample buffer zone is pre-embedded with lyophilized labeling reagents for different detection targets. The first sample buffer zone is located close to the sample dispensing area. When a liquid sample flows through the sample dispensing channel into the first sample buffer zone, the soluble reagent stopper briefly blocks the liquid sample before dissolving, achieving uniform dispensing of liquid samples across the multiple channels of the sample dispensing tray. The second sample buffer zone can be pre-embedded with lyophilized labeling antibodies or other reagents for different detection targets according to the detection requirements.

[0014] Furthermore, the sample pad is made of glass fiber or polyester fiber, and can be pretreated by soaking in a soaking solution before use, depending on the testing requirements.

[0015] Furthermore, the upper pressure fixing plate is provided with an inner pressure head and an outer pressure head. The inner pressure head presses on the inner end of the test strip and the outer periphery of the sample distribution plate. The inner end of the test strip extends beyond the test strip area of ​​the test strip distribution plate and onto the sample distribution channel of the sample distribution plate. The inner pressure head presses the inner end of the test strip onto the sample distribution channel. The outer pressure head presses on the outer end of the test strip (the absorbent pad at the end of the test strip).

[0016] Furthermore, the number of sample dispensing channels, test strip areas, inner pressure heads, and outer pressure heads are the same.

[0017] Furthermore, a snap-fit ​​position is provided above the upper pressure fixing plate for fixing the upper pressure fixing plate and the test strip distribution plate by snapping.

[0018] Furthermore, the upper pressure fixing plate has a hollowed-out observation area in the upper region of the test strip, which covers the detection line and control line of the test strip.

[0019] Furthermore, a chuck is provided at the bottom of the test strip distribution tray to position the detection device in the detection equipment, facilitating integrated detection with the result reading device.

[0020] A second aspect of the present invention provides a multi-channel sample detection method based on a lateral flow test strip, employing the multi-channel sample detection device based on a lateral flow test strip described in the first aspect, comprising the following steps:

[0021] (1) The liquid sample is dropped onto the sample pad. The hydrophilic membrane drives the liquid sample to flow faster to the first sample buffer zone, where it is briefly blocked by the soluble reagent plug pre-placed in the area.

[0022] (2) After the soluble reagent stopper is dissolved, the liquid sample continues to flow to the second sample buffer and mixes with the pre-embedded lyophilized labeling reagent. Then the mixed liquid flows to the corresponding test strip to achieve uniform distribution of liquid samples in multiple channels.

[0023] The results can be interpreted by the human eye by observing the test lines and control lines of the test strip in the hollowed-out observation area. Alternatively, the entire device can be placed in the result reading device and fixed by a chuck, enabling integrated testing with the result reading device.

[0024] The beneficial effects of this invention are:

[0025] The liquid sample from the sample pad of this invention flows through multiple sample distribution channels in the sample distribution tray to the corresponding test strip in the test strip distribution tray for detection. It does not rely on external power or complex equipment. It achieves rapid, uniform and automatic distribution of the liquid sample to be tested in multiple detection channels through microchannel optimization, structural design and capillary force, thereby effectively improving the consistency, reliability and ease of operation of multi-channel detection results. Attached Figure Description

[0026] To more clearly illustrate the technical solution of the present invention, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0027] Figure 1 The multi-channel sample detection device based on lateral flow test strips of the present invention explodes. Figure 1 ;

[0028] Figure 2The multi-channel sample detection device based on lateral flow test strips of the present invention explodes. Figure 2 ;

[0029] Figure 3 This is a plan view of the multi-channel sample detection device based on the side-flow test strip of the present invention;

[0030] Figure 4 This is a perspective view of the multi-channel sample detection device based on the side-flow test strip of the present invention;

[0031] The labels in the diagram are as follows: 1. Sample pad; 2. Upper pressure fixing plate; 21. Inner pressure head; 22. Outer pressure head; 23. Clip position; 24. Observation area; 3. Sample distribution plate; 31. Sample distribution area; 32. Sample distribution channel; 33. First sample buffer zone; 34. Second sample buffer zone; 4. Hydrophilic membrane; 5. Test strip distribution plate; 51. Test strip area; 52. Clamping plate. Detailed Implementation

[0032] The technical solution of the present invention will be clearly and completely described below with reference to specific embodiments. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0033] In this invention, unless otherwise stated, the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," indicating orientation or positional relationships, are merely for the convenience of describing the invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the invention. The terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. Furthermore, unless otherwise explicitly specified and limited, the terms "installed," "connected," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. For those skilled in the art, the specific meaning of the above terms in this patent can be understood according to the specific circumstances.

[0034] Reference Figures 1-4As shown, this embodiment relates to a multi-channel sample detection device based on a side-flow test strip, including a sample pad 1, an upper pressure fixing plate 2, a sample dispensing plate 3, a hydrophilic membrane 4, and a test strip dispensing plate 5; the sample dispensing plate 3 is provided with a sample dispensing area 31, and a plurality of sample dispensing channels 32 are arranged radially outward from the sample dispensing area 31 as the center; the sample dispensing area 31 is hollowed out, the sample pad 1 is placed in the sample dispensing area 31, and the hydrophilic membrane 4 is pasted on the back of the sample pad 1 and the sample dispensing plate 33, and the sample pad 1 is fixed to the sample dispensing area 31 by the hydrophilic membrane 4. Inside, it is connected to the sample distribution channel 32, so that the liquid sample can flow from the sample pad 1 to the sample distribution channel 32 of the sample distribution plate 3 during testing; the test strip distribution plate 5 is sleeved on the outer periphery of the sample distribution plate 3, and the test strip distribution plate 5 is provided with a test strip area 51 along the extension direction of the sample distribution channel 32 for placing the test strip. The inner end of the test strip is connected to the sample distribution channel 32, so that the liquid sample flowing through the sample distribution channel 32 can flow into the test strip; the upper pressure fixing plate 2 is pressed on the outer periphery of the sample distribution plate 33 and on the test strip distribution plate 5 to fix the test strip.

[0035] In this embodiment, the liquid sample from sample pad 1 flows through multiple sample distribution channels 32 of sample distribution tray 3 to the corresponding test strip in test strip distribution tray 5 for detection. This eliminates the need for external power and complex equipment; through microchannel optimization, structural design, and capillary force, it achieves rapid, uniform, and automatic distribution of the liquid sample in multiple detection channels, effectively improving the consistency, reliability, and ease of operation of multi-channel detection results. Furthermore, the number of sample distribution channels 32 and their corresponding test strips can be adjusted according to actual needs, offering high adaptability.

[0036] In a preferred embodiment, the sample distribution tray 3 is made of cellulose paper, and the sample distribution channels 32 of the sample distribution tray 3 are formed by wax printing. A first sample buffer zone 33 and a second sample buffer zone 34 are provided on the sample distribution channels 32. The first sample buffer zone 33 is pre-filled with a soluble reagent stopper, and the second sample buffer zone 34 is pre-embedded with lyophilized labeling reagents for different detection targets. The first sample buffer zone 33 is located close to the sample distribution area 31. When a liquid sample flows through the sample distribution channels 32 to the first sample buffer zone 33, the soluble reagent stopper briefly blocks the liquid sample and then dissolves, achieving uniform distribution of liquid samples across the multiple channels of the sample distribution tray 3. The second sample buffer zone 34 can be pre-embedded with lyophilized labeling antibodies or other reagents for different detection targets according to detection requirements. The sample pad 1 is made of glass fiber or polyester fiber, and can be pre-treated with an soaking solution before use according to detection requirements.

[0037] In a preferred embodiment, the upper pressure fixing plate 2 is provided with an inner pressure head 21 and an outer pressure head 22. The inner pressure head 21 presses against the inner end of the test strip and the outer periphery of the sample distribution plate 33. The inner end of the test strip extends beyond the test strip area 51 of the test strip distribution plate 5 and onto the sample distribution channel 32 of the sample distribution plate 3. The inner pressure head 21 presses the inner end of the test strip onto the sample distribution channel 32. The outer pressure head 22 presses against the outer end of the test strip (the absorbent pad at the end of the test strip). The number of sample distribution channels 32, test strip area 51, inner pressure head 21, and outer pressure head 22 are the same. A snap-fit ​​position 23 is provided above the upper pressure fixing plate 2 for fixing the upper pressure fixing plate 2 and the test strip distribution plate 5 by a clip-type snap-fit.

[0038] In a preferred embodiment, the upper pressure fixing plate 2 has a perforated observation area 24 above the test strip, which covers the test lines and control lines of the test strip. The test strip distribution plate 5 has a chuck 52 at its bottom for positioning the detection device within the detection equipment, facilitating integrated testing with the result reading device. Results can be interpreted visually by observing the test lines and control lines of the test strip within the perforated observation area 24, or the entire device can be placed within the result reading device and secured by the chuck 52, enabling integrated testing with the result reading device.

[0039] Another embodiment relates to a multi-channel sample detection method based on a lateral flow test strip, employing the multi-channel sample detection device based on a lateral flow test strip as described in the first aspect, comprising the following steps:

[0040] (1) The liquid sample is dropped onto the sample pad 1, and the hydrophilic membrane 4 drives the liquid sample to flow faster to the first sample buffer 33, and is temporarily blocked by the soluble reagent plug pre-placed in this area.

[0041] (2) After the soluble reagent stopper is dissolved, the liquid sample continues to flow into the second sample buffer 34, where it mixes with the pre-embedded lyophilized labeling reagent. The mixed liquid then flows onto the corresponding test strip, achieving uniform distribution of liquid samples across multiple channels. The results can be interpreted by visually observing the test lines and control lines of the test strip within the perforated observation area 24. Alternatively, the entire device can be placed within the result reading device and fixed using the chuck 52, enabling integrated testing with the result reading device.

[0042] In summary, the liquid sample from the sample pad of this invention flows through multiple sample distribution channels in the sample distribution tray to the corresponding test strip in the test strip distribution tray for detection. It does not rely on external power or complex equipment. Only through microchannel optimization, structural design and capillary force, it achieves rapid, uniform and automatic distribution of the liquid sample to be tested in multiple detection channels, thereby effectively improving the consistency, reliability and ease of operation of multi-channel detection results.

[0043] The present invention has been described in detail above with reference to specific embodiments and exemplary examples; however, these descriptions should not be construed as limiting the present invention. Those skilled in the art will understand that various equivalent substitutions, modifications, or improvements can be made to the technical solutions and embodiments of the present invention without departing from the spirit and scope of the invention, and all such modifications and improvements fall within the scope of the present invention. The scope of protection of the present invention is defined by the appended claims.

Claims

1. A multi-channel sample detection device based on a side-flow test strip, characterized in that, Includes sample pad, pressure plate, sample dispensing plate, hydrophilic membrane, and test strip dispensing plate; The sample distribution disk is provided with a sample distribution area, and several sample distribution channels are arranged radially outward from the sample distribution area as the center; The sample pad is placed in the sample dispensing area, and the hydrophilic membrane is adhered to the back of the sample pad and the sample dispensing tray; The test strip distribution disk is fitted around the outer periphery of the sample distribution disk. The test strip distribution disk has a test strip area along the extension direction of the sample distribution channel for placing test strips. The inner end of the test strip is connected to the sample distribution channel. The upper pressure fixing plate presses on the outer periphery of the sample distribution plate and the test strip distribution plate.

2. The multi-channel sample detection device based on lateral flow test strips as described in claim 1, characterized in that, The sample dispensing tray is made of cellulose paper, and the sample dispensing channels of the sample dispensing tray are formed by wax printing.

3. The multi-channel sample detection device based on lateral flow test strips as described in claim 2, characterized in that, The sample distribution channel is provided with a first sample buffer and a second sample buffer. The first sample buffer is pre-filled with a soluble reagent stopper, and the second sample buffer is pre-embedded with lyophilized labeling reagents for different detection targets.

4. The multi-channel sample detection device based on lateral flow test strips as described in claim 1, characterized in that, The sample pad is made of glass fiber or polyester fiber.

5. The multi-channel sample detection device based on lateral flow test strips as described in claim 1, characterized in that, The upper pressure fixing plate is equipped with an inner pressure head and an outer pressure head. The inner pressure head presses against the inner end of the test strip and the outer periphery of the sample distribution plate, while the outer pressure head presses against the outer end of the test strip.

6. The multi-channel sample detection device based on lateral flow test strips as described in claim 5, characterized in that, The number of sample distribution channels, test strip areas, inner pressure heads, and outer pressure heads are the same.

7. The multi-channel sample detection device based on lateral flow test strips as described in claim 1, characterized in that, A snap-fit ​​position is provided above the upper pressure fixing plate for fixing the upper pressure fixing plate and the test strip distribution plate by snapping.

8. The multi-channel sample detection device based on lateral flow test strips as described in claim 1, characterized in that, The upper pressure fixing plate has a hollowed-out observation area in the area above the test strip.

9. The multi-channel sample detection device based on lateral flow test strips as described in claim 1, characterized in that, The bottom of the test strip distribution tray is equipped with a chuck for positioning the detection device in the detection equipment.

10. A multi-channel sample detection method based on a lateral flow test strip, characterized in that, The multi-channel sample detection device based on lateral flow test strips as described in any one of claims 1-9 includes the following steps: (1) The liquid sample is dropped onto the sample pad. The hydrophilic membrane drives the liquid sample to flow faster to the first sample buffer zone, where it is briefly blocked by the soluble reagent plug pre-placed in the area. (2) After the soluble reagent stopper is dissolved, the liquid sample continues to flow to the second sample buffer and mixes with the pre-embedded lyophilized labeling reagent. Then the mixed liquid flows to the corresponding test strip to achieve uniform distribution of liquid samples in multiple channels.

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