POCT (Point of Care Testing) detection equipment and detection method for detecting nutrient substances

Abstract

The invention belongs to the technical field of detection equipment, and particularly discloses POCT (Point of Care Testing) detection equipment for nutrient substance detection, which comprises a bearing part, a translation assembly, a liquid preparation mechanism, a moving mechanism, a detection needle assembly and a detection unit, the bearing part comprises a bottom plate and a mounting frame arranged on the bottom plate; the translation assembly comprises a kit placing rack and a driving assembly, the driving assembly is arranged on the bottom plate, and the driving assembly is used for driving the kit placing rack to move in the length direction of the bottom plate. According to the POCT detection equipment for nutrient substance detection, full-process automatic operation is achieved by integrating the translation assembly, the liquid preparation mechanism and the automatic movement mechanism, a liquid path system is simplified through modular design, and the POCT detection equipment has the advantages that the detection automation degree is improved, the operation process is simplified, and the equipment size and the operation cost are reduced.

Description

Technical Field

[0001] This invention relates to the field of testing equipment technology, and in particular to a point-of-care testing (POCT) device and method for detecting nutrients. Background Technology

[0002] Trace element analyzers are mainly used to detect the content of trace elements in the human body. Currently, the mainstream products on the market include three main categories: electrochemical analysis, atomic absorption spectrometry, and intracellular plasma mass spectrometry (ICP-MS). Among them, atomic absorption spectrometry and ICP-MS equipment are high-end products, expensive and complex to operate, and are mainly used in large medical institutions.

[0003] Electrochemical analysis instruments are widely used in hospitals and community health service centers at all levels due to their advantages of easy operation, accurate results and low consumable costs.

[0004] However, existing electrochemical trace element analyzers have significant technical shortcomings: First, they lack automation, requiring specialized personnel to complete complex operating procedures; second, the instruments are bulky and have complex liquid circuit systems, making them difficult to adapt to the testing needs of different scenarios; third, the consumption of consumables during equipment operation is high, increasing the operating costs of medical institutions. Especially in POCT (point-of-care testing) scenarios, existing equipment cannot meet the requirements for rapid, convenient, and accurate testing. These problems severely restrict the promotion and application of trace element detection technology in primary healthcare institutions.

[0005] In summary, existing electrochemical trace element analyzers suffer from problems such as complex operation, high maintenance costs, and difficulty in meeting real-time detection needs. Summary of the Invention

[0006] This invention provides a POCT detection device for nutrient detection, which can solve the problems of existing electrochemical trace element analyzers, such as complex operation, high maintenance costs, and difficulty in meeting the needs of real-time detection.

[0007] In a first aspect, the present invention provides a POCT detection device for nutrient detection, comprising: A support member, the support member including a base plate and a mounting bracket disposed on the base plate; A translation component, comprising a reagent kit holder and a drive component, wherein the drive component is disposed on a base plate and is used to drive the reagent kit holder to move along the length direction of the base plate; A liquid preparation mechanism, comprising a movable connecting seat and a sampling gun body disposed on the movable connecting seat; A moving mechanism is mounted on a mounting frame and is used to drive the liquid dispensing mechanism to move along the height direction of the mounting frame. The detection needle assembly includes a drive mechanism and a needle fixing plate. The drive mechanism is mounted on the mounting bracket and is used to drive the needle fixing plate to move. The needle fixing plate is provided with an outlet needle and an inlet needle. The detection unit is connected to the liquid outlet needle and the liquid inlet needle respectively through the liquid circuit module.

[0008] This invention provides a POCT detection device for nutrient detection, which, compared with the prior art, has, but is not limited to, the following beneficial effects: The POCT testing equipment for nutrient detection includes a base plate and a mounting frame, forming a basic support structure. The translation component achieves lateral positioning of the reagent kit through the drive component. The liquid preparation mechanism is equipped with a liftable sampling gun to complete liquid collection. The moving mechanism controls the vertical movement of the sampling gun. The detection needle assembly achieves liquid transfer through a dual-needle structure. The detection unit is connected to the detection needle through a liquid circuit module to complete the analysis.

[0009] The device comprises several components: a support frame for the testing equipment, which can be a combination of a base plate and a mounting bracket, with the bracket vertically fixed to the edge of the base plate to provide a mounting reference for other components; a translation component for horizontal movement of the reagent kit, which can be a drive component for precise positioning of the reagent kit between testing stations; a dispensing mechanism for liquid transfer, which can be a movable connector housing the sampling gun and a moving mechanism for aspiration and pipetting operations; and a probe assembly for controlling the height of the dispensing mechanism to ensure the sampling gun switches between different height stations. Finally, a dual-needle structure connecting the testing units, with a drive mechanism controlling the needle's lifting and lowering to achieve the aspiration and expulsion of the testing liquid.

[0010] This POCT testing equipment for nutrient detection achieves fully automated operation by integrating translation components, liquid dispensing mechanisms, and automated moving mechanisms. It adopts a modular design to simplify the liquid circuit system, which has the advantages of improving the degree of automation of detection, simplifying the operation process, and reducing equipment size and operating costs.

[0011] Furthermore, the POCT testing device for nutrient detection also includes a control component, which is connected to the translation component, the liquid preparation mechanism, the moving mechanism, the detection needle assembly, and the detection unit.

[0012] Furthermore, the drive assembly includes a linear guide rail A, a stepper motor A, a drive wheel A, a synchronous belt A, a driven wheel A, and a driven frame, wherein the driven frame, the stepper motor A, and the linear guide rail A are all mounted on the base plate; The driving wheel A is located at the output end of the stepper motor A, the driven wheel A is located on the driven frame, and the synchronous belt A is wound around the driving wheel A and the driven wheel A. The reagent kit holder has a connecting block on its side, and the connecting block is located on the synchronization belt A; The reagent kit holder is slidably mounted on the linear guide rail A.

[0013] Furthermore, the moving mechanism includes a top plate A, a stepper motor B, a synchronous belt B, a driving wheel B, a driven wheel B, and a lead screw A, wherein the top plate A is disposed on the side of the mounting frame away from the base plate, and the synchronous belt B is wound around the driving wheel B and the driven wheel B; The stepper motor B is mounted on the top plate A, and the driving wheel B and the driven wheel B are both mounted on the top plate A on the side away from the stepper motor B. The output end of the stepper motor B passes through the top plate A and is connected to the drive wheel B; The lead screw A passes through the top plate A and is connected to the driven wheel B; The lead screw A is fitted with a lead screw slider A, and the lead screw slider A is equipped with a sampling gun up-and-down sliding slider. The movable connecting seat is located on the sampling gun up-and-down sliding slider.

[0014] Furthermore, the mounting bracket is also provided with a guide plate, and one end of the lead screw A away from the driven wheel B is located on the guide plate.

[0015] Furthermore, the liquid dispensing mechanism includes a top plate B, a stepper motor C, a synchronous belt C, a driving wheel C, a driven wheel C, and a lead screw B, wherein the top plate B is disposed on the movable connecting seat, and the synchronous belt C is wound around the driving wheel C and the driven wheel C; The stepper motor C is mounted on the top plate B, and the driving wheel C and the driven wheel C are both mounted on the top plate B on the side away from the stepper motor C. The output end of the stepper motor C passes through the top plate B and is connected to the drive wheel C; The lead screw B passes through the top plate B and is connected to the driven wheel C; The lead screw B is fitted with a lead screw slider B, and the lead screw slider B is provided with a sampling gun sliding block; The sampling gun body is fitted with a fixing seat, which is located on the sliding block of the sampling gun.

[0016] Furthermore, the movable connecting seat is provided with a linear guide rail B, and the sampling gun sliding block is slidably disposed on the linear guide rail B.

[0017] Furthermore, the fixing base is also provided with a connector, the connector is provided with a TIP head push plate, and the TIP head push plate is provided with an opening block; The TIP head push plate has mounting holes that mate with the sampling gun body.

[0018] Furthermore, the driving mechanism includes a detection mechanism fixing plate, a stepper motor D, a first cam, a second cam, a needle sliding block, and a linear guide rail C, wherein one side of the needle sliding block is slidably disposed on the linear guide rail C, and the other side of the needle sliding block is connected to the needle fixing plate; The stepper motor D is located on one side of the detection mechanism fixing plate, and the linear guide rail C is located on the other side of the detection mechanism fixing plate; The output end of the stepper motor D passes through the detection mechanism fixing plate and is connected to the first cam; The end of the first cam is connected to the second cam, and the needle sliding block is provided with a straight sliding groove that cooperates with the second cam.

[0019] Secondly, the present invention also provides a detection method for a POCT detection device for nutrient detection, the method comprising the following steps: S1, Activation of the detection unit Place the reagent kit into the reagent kit rack. Drive the reagent kit rack along the length of the base plate using the translation component. Position the activation solution tank in the reagent kit below the solution preparation mechanism. Drive the dispensing needle and the inlet needle on the detection needle assembly to insert them into the activation solution tank on the reagent kit. Then, deliver the activation solution into the detection unit. The dispensing needle and the inlet needle are lifted, and the detection unit begins activation. S2, Cleaning of the detection unit After the detection unit is activated, the reagent kit holder continues to move to the cleaning solution tank position on the reagent kit. Through the drive mechanism on the detection needle assembly, the drive needle fixing plate is lowered, which drives the liquid outlet needle and the liquid inlet needle downward to deliver the cleaning solution into the detection kit. The liquid outlet needle and the liquid inlet needle are then raised, and the detection unit begins to clean. S3, Extraction of reaction solution After cleaning, the TIP on the reagent kit moves to the bottom of the sampling gun body. The moving mechanism drives the sampling gun body on the dispensing mechanism to extract the TIP head. After completion, it is lifted up. The reagent kit continues to move so that the sample slot on it is below the sampling gun body. The moving mechanism drives the dispensing mechanism to descend. The TIP head on the sampling gun body is inserted into the sample slot to extract the sample from the sample slot. After extraction, the moving mechanism drives the dispensing mechanism to lift up. The bottom liquid well on the reagent kit moves to the bottom of the sampling gun body. The moving mechanism drives the liquid preparation mechanism to descend. The TIP head is inserted into the bottom liquid well to draw out the liquid in the bottom liquid well. After the liquid is drawn out, the moving mechanism drives the liquid preparation mechanism to rise. The reaction well on the reagent kit moves to the bottom of the sampling gun body. The moving mechanism drives the dispensing mechanism to descend, the TIP head is inserted into the reaction well, and the liquid in the TIP head is ejected. After completion, the moving mechanism drives the dispensing mechanism to rise. S4, Detection The reaction well on the reagent kit is moved under the injection needle, the injection needle descends and is inserted into the reaction well, delivering the liquid in the reaction well to the detection unit. The injection needle is then lifted, and the detection unit begins to operate. S5, Reset The bottom liquid well on the kit moves to the position below the injection needle. The injection needle descends and inserts into the bottom liquid well, transferring the liquid from the bottom liquid well to the detection unit. The injection needle then rises. The kit moves to the starting position, completing the detection. Attached Figure Description

[0020] The accompanying drawings, which are included to provide a further understanding of the invention and form part of this application, illustrate exemplary embodiments of the invention and, together with their description, serve to explain the invention and do not constitute an undue limitation thereof. In the drawings: Figure 1 A schematic diagram of the structure of a POCT detection device for nutrient detection provided by the present invention; Figure 2 A schematic diagram of the structure of a POCT detection device for nutrient detection provided by the present invention; Figure 3 A schematic diagram of the translation component of a POCT detection device for nutrient detection provided by the present invention; Figure 4 A schematic diagram of the moving mechanism of a POCT detection device for nutrient detection provided by the present invention; Figure 5 A schematic diagram of the solution preparation mechanism of a POCT detection device for nutrient detection provided by the present invention; Figure 6 This is a schematic diagram of the detection needle assembly structure of a POCT detection device for nutrient detection provided by the present invention.

[0021] Explanation of reference numerals in the attached figures: 1. Supporting Components; 101. Base Plate; 102. Mounting Frame; 103. Guide Plate; 104. Vertical Plate A; 105. Vertical Plate B; 2. Translation Components; 201. Reagent Kit Placement Rack; 202. Linear Guide Rail A; 203. Stepper Motor A; 204. Driving Wheel A; 205. Synchronous Belt A; 206. Driven Wheel A; 207. Driven Frame; 208. Reagent Kit; 3. Moving Mechanism; 300. Top Plate A; 301. Stepper Motor B; 302. Synchronous Belt B; 303. Driving Wheel B; 304. Driven Wheel B; 305. Lead Screw A; 306. Lead Screw Slider A; 307. Sampling Gun Up / Down Moving Slider; 308. Linear Guide Rail D; 4. Solution Dispensing Mechanism; 400. Top Plate B; 401. Moving Connector; 402. Stepper motor C; 403. Synchronous belt C; 404. Drive wheel C; 405. Driven wheel C; 406. Lead screw B; 407. Lead screw slider B; 408. Sampling gun slider block; 409. Fixing base; 410. Linear guide rail B; 411. TIP head push plate; 412. Opening block; 413. Connector; 414. Sampling gun body; 5. Detection needle assembly; 501. Needle fixing plate; 502. Discharge needle; 503. Inlet needle; 504. Detection mechanism fixing plate; 505. Stepper motor D; 506. First cam; 507. Second cam; 508. Needle slider block; 509. Linear guide rail C; 510. Linear slide; 6. Peristaltic pump; 7. Detection unit; 8. Power switch; 9. Control component. Detailed Implementation

[0022] To make the objectives, technical solutions, and advantages of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings showing multiple embodiments according to this application. It should be understood that the described embodiments are only a part of the embodiments of this application, and not all of the embodiments. All other embodiments obtained by those skilled in the art based on the embodiments described in this application without creative effort will fall within the scope of protection of this application.

[0023] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the terminology used in the description of this application is for the purpose of describing specific embodiments only and is not intended to limit this application; the terms "comprising," "including," "having," "containing," etc., in the description, claims, and accompanying drawings of this application are open-ended terms. Therefore, "comprising," "including," or "having" refers to, for example, a method or apparatus having one or more steps or elements, but is not limited to having only these one or more elements. The terms "first," "second," etc., in the description, claims, or accompanying drawings of this application are used to distinguish different objects, not to describe a specific order or hierarchy. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, unless otherwise stated, "a plurality of" means two or more.

[0024] In the description of this invention, it should be understood that the terms "upper", "lower", "left", "right", "front", "rear", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0025] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "attachment" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0026] It should be emphasized that when the term "comprising / including" is used in this specification, it is used to explicitly indicate the presence of the stated feature, integer, step, or component, but does not exclude the presence or addition of one or more other features, integers, steps, parts, or groups of features, integers, steps, or parts.

[0027] In this application, the term "and / or" is merely a description of the relationship between related objects, indicating that three relationships can exist. For example, B and / or C can represent: B existing alone, B and C existing simultaneously, or C existing alone. Additionally, in this application, the character " / " generally indicates that the preceding and following related objects have an "or" relationship.

[0028] In existing technologies, electrochemical trace element analyzers generally suffer from low automation, complex liquid circuit systems, and large device size. Traditional instruments rely on manual operation to complete reagent positioning, sample transfer, and detection processes, resulting in limited detection efficiency. Furthermore, the complex liquid circuit systems require frequent maintenance and replacement of consumables. In actual testing, it is necessary to quickly complete the nutrient detection of batches of samples, but existing equipment is difficult to meet the demand for real-time detection due to its complex operation and high maintenance costs.

[0029] To address these issues, research revealed that automated positioning and liquid transfer are key to simplifying operations. Analysis showed that existing equipment lacked an integrated motion control system, resulting in independent operation of each functional module. After repeated verification, it was determined that the three core processes of reagent positioning, sampling, and detection execution would be mechanically linked, constructing a collaborative working mechanism in a spatial dimension.

[0030] Based on this, such as Figures 1 to 2 As shown, an embodiment of the first aspect of the present invention provides a POCT detection device for nutrient detection, including a carrier 1, a translation component 2, a liquid dispensing mechanism 4, a moving mechanism 3, a detection needle assembly 5, and a detection unit 7; Specifically, the carrier 1 includes a base plate 101 and a mounting bracket 102 disposed on the base plate 101; the translation component 2 includes a reagent kit placement rack 201 and a driving component, the driving component being disposed on the base plate 101 and used to drive the reagent kit placement rack 201 to move along the length direction of the base plate 101; the liquid preparation mechanism 4 includes a movable connecting seat 401 and a sampling gun body 414 disposed on the movable connecting seat 401; the moving mechanism 3 is disposed on the mounting bracket 102 and used to drive the liquid preparation mechanism 4 to move along the height direction of the mounting bracket 102; the detection needle assembly 5 includes a driving mechanism and a needle fixing plate 501, the driving mechanism being disposed on the mounting bracket 102 and used to drive the needle fixing plate 501 to move, the needle fixing plate 501 being provided with an outlet needle 502 and an inlet needle 503; the detection unit 7 is connected to the outlet needle 502 and the inlet needle 503 respectively through a liquid circuit module.

[0031] The carrier 1 includes a base plate 101 and a mounting bracket 102, forming a basic support structure; the translation component 2 achieves the lateral positioning of the reagent kit 208 through the drive component; the liquid preparation mechanism 4 is equipped with a liftable sampling gun to complete liquid collection; the moving mechanism 3 controls the vertical movement of the sampling gun; the detection needle assembly 5 achieves liquid transfer through a double needle structure; and the detection unit 7 is connected to the detection needle through the liquid circuit module to complete the analysis.

[0032] The supporting component 1 serves as the basic frame supporting the operation of the testing equipment. Specifically, it can be a combination of a base plate 101 and a mounting bracket 102. The mounting bracket 102 is vertically fixed to the edge of the base plate 101, providing a mounting reference for other components. The translation component 2 is a device for horizontal movement of the reagent kit 208. Specifically, it can be a drive component to achieve precise positioning of the reagent kit 208 between testing stations. The liquid preparation mechanism 4 is a device for performing liquid transfer. Specifically, it can be a movable connecting seat 401 carrying the sampling gun body 414 and a moving mechanism 3 to complete liquid aspiration and transfer operations. The moving mechanism 3 is a device for controlling the height of the liquid preparation mechanism 4, ensuring that the sampling gun switches between different height stations. The detection needle assembly 5 is a device for realizing the flow path of the detection liquid. Specifically, it can be a dual-needle structure connecting the detection unit 7, with the needle body raised and lowered by a drive mechanism to achieve the aspiration and discharge of the detection liquid.

[0033] Specifically, the base plate 101 serves as the basic platform for the equipment, supporting all functional modules, while the mounting frame 102 provides vertical structural support. The reagent placement rack 201 moves along the length of the base plate 101 via the translation component 2, positioning the target reagent well below the sampling gun. The moving mechanism 3 drives the liquid preparation mechanism 4 to move vertically along the mounting frame 102, ensuring the sampling gun precisely approaches the reagent well to complete the liquid aspiration operation. The drive mechanism on the detection needle assembly 5 drives the dual needles to insert into the reaction well, and the liquid path module delivers the reaction liquid to the detection unit 7. All components operate in coordination through the control component, forming a closed-loop detection process.

[0034] Compared to existing technologies, traditional equipment requires manual operation of three independent steps: reagent positioning, sample transfer, and detection execution. This solution, however, achieves automatic reagent positioning through the translation component 2, automatic sample transfer through the linkage of the dispensing mechanism 4 and the moving mechanism 3, and automation of the detection process through the cooperation of the detection needle component 5 and the liquid path module. Existing equipment employs a complex liquid path system with multiple pumps and valves; this solution simplifies the liquid path design through a dual-needle structure and the coordination of the liquid path module.

[0035] Through the above technical solutions, this application realizes fully automated control of reagent positioning, sample transfer, and detection execution, eliminating human operation errors; adopts an integrated mechanical structure to replace the traditional discrete liquid circuit system, effectively reducing the size of the equipment; reduces consumable consumption and maintenance costs through the reusable detection needle component 5; and has a compact overall structure, making it suitable for space-constrained scenarios such as bedside testing and community healthcare.

[0036] like Figures 1 to 6 As shown, in some embodiments of the present invention, the detection unit 7 can be designed using an electrochemical three-electrode system, integrating a miniaturized detection module. It is equipped with a working electrode and a reference electrode arranged in parallel, and a counter electrode positioned opposite the working electrode. Different electrodes can be selected for different nutrients, and it is not limited to a single type of electrode. That is, the detection unit 7 can be designed using an electrochemical three-electrode system, or it can employ a two-electrode or five-electrode system, and it is not limited to a single type of electrode. (For example, the working electrode includes a glassy carbon electrode, a gold electrode, or a platinum electrode; the counter electrode includes a platinum sheet or graphite; the reference electrode includes a saturated calomel electrode or Ag / AgCl).

[0037] like Figures 1 to 6 As shown, in some embodiments of the present invention, the driving component is further equipped with a photoelectric switch to limit the moving distance of the reagent kit holder 201. Specifically, the photoelectric switch can precisely control the moving range of the reagent kit holder, ensuring the accuracy and stability of reagent positioning. When the reagent kit holder moves to a preset position, the photoelectric switch sends a signal, and the driving component immediately stops driving, thereby avoiding problems such as reagent spillage or inaccurate positioning caused by excessive movement of the reagent kit holder. This design further improves the automation level and detection accuracy of the equipment, providing a strong guarantee for the rapid and accurate detection of nutrients.

[0038] like Figures 1 to 6 As shown, in some embodiments of the present invention, a power switch 8 is also provided on the base plate 101. The power switch 8 is used to control the power supply of the equipment. Specifically, the power switch 8 can adopt a waterproof and dustproof design to ensure stable operation in complex environments. Its position is set in an easily accessible area on the edge of the base plate 101, making it convenient for users to quickly turn the equipment on or off. At the same time, the power switch 8 integrates an overload protection module. When the operating current of the equipment is abnormal, it can automatically cut off the power supply to prevent circuit damage and effectively extend the service life of the equipment.

[0039] like Figures 1 to 6 As shown, in some embodiments of the present invention, the mounting bracket 102 includes two parallel vertical plates A 104 and B 105; both vertical plates A 104 and B 105 are mounted on the base plate 101.

[0040] A stable support structure is formed between vertical plate A 104 and vertical plate B 105 for installing and fixing the liquid dispensing mechanism, detection needle assembly, and detection unit. Vertical plate A 104 and vertical plate B 105 can be made of high-strength alloy material to ensure they can withstand large loads without deformation during equipment operation.

[0041] like Figures 1 to 6 As shown, in some embodiments of the present invention, the support member 1 refers to the basic frame that supports the operation of the testing equipment. Specifically, it can be a combination structure of a base plate 101 and a mounting frame 102 formed by welding metal plates. The mounting frame 102 is vertically fixed to the edge of the base plate 101 to provide an installation reference for other components.

[0042] like Figures 1 to 6 As shown, in some embodiments of the present invention, the POCT detection device for nutrient detection further includes a control component 9, which is connected to the translation component 2, the liquid preparation mechanism 4, the moving mechanism 3, the detection needle assembly 5, and the detection unit 7.

[0043] Among them, the control component 9 refers to the central control system used to coordinate the actions of various components of the detection equipment. Specifically, it can be implemented using a microcontroller or a programmable logic controller, and can send instruction signals to each actuator through a preset program.

[0044] Specifically, for example, the control component 9 can establish a communication connection with each actuator through a signal transmission interface. When the device is started, the control component first sends a displacement command to the translation component 2, driving the reagent kit holder 201 to move to the predetermined position; then, it controls the moving mechanism 3 to drive the liquid preparation mechanism 4 to descend to the sampling position, triggering the sampling gun to complete liquid aspiration; next, it controls the drive mechanism of the detection needle assembly 5 to perform a puncture action, allowing the inlet needle 503 and outlet needle 502 to enter the reaction container; finally, the liquid to be tested is delivered to the detection unit 7 through the liquid circuit module, while simultaneously receiving real-time data fed back by the detection unit 7. Throughout the process, the control component achieves coordinated operation of each module through timing control.

[0045] Compared to existing technologies, traditional equipment requires manual operation of pipetting, positioning, and detection steps, while this solution achieves full-process automation through an integrated control system. Existing technologies use discrete control modules, resulting in bulky equipment, while this solution simplifies the electrical connection structure by using a unified control component. Conventional equipment relies on complex manual fluid circuit switching, while this solution achieves precise fluid circuit scheduling through programmed control. Specifically, through the above technical solutions, this application achieves fully automated control of the detection process, eliminating human error. The coordinated action of each actuator significantly improves detection efficiency and significantly shortens the single detection cycle. Furthermore, the integrated control architecture reduces equipment size, making it more suitable for point-of-care testing scenarios. The programmed control mode reduces consumable consumption and avoids the risk of cross-contamination caused by the complex fluid circuits of traditional equipment.

[0046] like Figures 1 to 6As shown, in some embodiments of the present invention, the drive assembly includes a linear guide rail A 202, a stepper motor A 203, a drive wheel A 204, a synchronous belt A 205, a driven wheel A 206, and a driven frame 207. The driven frame 207, the stepper motor A 203, and the linear guide rail A 202 are all mounted on the base plate 101. Among them, the driving wheel A 204 is located at the output end of the stepper motor A 203, the driven wheel A 206 is located on the driven frame 207, and the synchronous belt A 205 is wound around the driving wheel A 204 and the driven wheel A 206; The reagent kit holder 201 has a connecting block on its side, which is mounted on the synchronous belt A 205; the reagent kit holder 201 is slidably mounted on the linear guide rail A 202; The linear guide rail A 202 is a guiding component used to support the linear movement of the reagent kit holder 201. It can be implemented using a high-precision ball-bearing linear guide rail, providing a stable sliding path for the reagent kit holder 201. The stepper motor A 203 is the power source driving the synchronous belt, which can be implemented using a two-phase hybrid stepper motor. Its function is to control the precise displacement of the synchronous belt through pulse signals. The synchronous belt A 205 is the transmission component connecting the driving pulley and the driven pulley. It can be implemented using a rubber-based steel wire embedded synchronous belt, converting the rotational motion of the stepper motor into linear displacement. The driven frame 207 is the fixed structure supporting the driven pulley, which can be formed from aluminum alloy profiles, providing rigid support for the synchronous belt transmission system. The connecting block is the connecting component between the reagent kit holder 201 and the synchronous belt, which can be fixed with stainless steel bolts, transmitting the linear motion of the synchronous belt to the reagent kit holder 201.

[0047] Specifically, stepper motor A 203 drives drive wheel A 204 to rotate via its output shaft, and synchronous belt A 205 forms a closed-loop transmission between drive wheel A 204 and driven wheel A 206. A connecting block is rigidly fixed to the straight section of synchronous belt A 205. When stepper motor A 203 receives a control signal, synchronous belt A 205 drives the connecting block to move along the extension direction of linear guide rail A 202, thereby driving reagent kit holder 201 to complete precise positioning. The ball bearing slider of linear guide rail A 202 and the sliding contact surface of reagent kit holder 201 form a low-friction kinematic pair, ensuring no jamming during displacement. Driven frame 207 is fixed to a preset position on base plate 101 by bolts, providing stable rotational support for driven wheel A 206.

[0048] This solution employs a transmission method combining synchronous belts and linear guides, simplifying the mechanical structure and reducing wear on moving parts while ensuring positioning accuracy. Synchronous belt drives require no lubrication and significantly reduce operating noise, making them more suitable for medical testing scenarios. Through this technical solution, high-precision automated positioning control of the reagent kit holder 201 is achieved, solving the inefficiency problem caused by manual operation in traditional equipment. The combination of the synchronous belt drive system and the linear guides makes the equipment operate more smoothly and reliably, reducing testing errors caused by mechanical vibration. The modular design of the drive components facilitates maintenance and replacement, effectively reducing equipment operating costs.

[0049] like Figures 1 to 6 As shown, in some embodiments of the present invention, the moving mechanism 3 includes a top plate A 300, a stepper motor B 301, a timing belt B 302, a drive wheel B 303, a driven wheel B 304, and a lead screw A 305. The top plate A 300 is disposed on the side of the mounting bracket 102 away from the bottom plate 101, and the timing belt B 302 is wound around the drive wheel B 303 and the driven wheel B 304. Stepper motor B 301 is located at the bottom of top plate A 300, while drive wheel B 303 and driven wheel B 304 are located at the top of top plate A 300. The output end of stepper motor B 301 passes through top plate A 300 and is connected to drive wheel B 303; The lead screw A 305 passes through the top plate A 300 and is connected to the driven wheel B 304; A lead screw A 305 is fitted with a lead screw slider A 306, and a sampling gun up-and-down moving slider 307 is provided on the lead screw slider A 306. A movable connecting seat 401 is provided on the sampling gun up-and-down moving slider 307. The top plate A 300 is a support plate that carries the main structure of the moving mechanism 3, providing a mounting reference surface for the stepper motor B 301 and transmission components. The stepper motor B 301 is the power source driving the vertical movement of the dispensing mechanism 4; it can be implemented using a two-phase hybrid stepper motor, with the rotation angle controlled by pulse signals to precisely adjust the lifting stroke. The synchronous belt B 302 is the transmission component that transmits power; it can be implemented using a polyurethane synchronous belt with steel reinforcing wire, used to transmit the rotational motion of the stepper motor B 301 to the driven wheel B 304. The lead screw A 305 is a precision transmission component that converts rotational motion into linear motion; it can be implemented using a ball screw pair, and its cooperation with the lead screw slider A 306 enables the height adjustment of the dispensing mechanism 4.

[0050] Specifically, when stepper motor B 301 receives a control signal, its output shaft drives the driving wheel B 303 to rotate, which in turn drives the driven wheel B 304 to rotate synchronously via synchronous belt B 302. Since lead screw A 305 is coaxially connected to driven wheel B 304, the rotational motion of driven wheel B 304 is converted into the rotational motion of lead screw A 305. The lead screw slider A 306, sleeved on lead screw A 305, then undergoes axial displacement, causing the sampling gun's up-and-down sliding slider 307 to move along the height direction of mounting bracket 102. The movable connecting seat 401 is fixedly connected to the sampling gun's up-and-down sliding slider 307, thereby achieving precise positioning of the liquid dispensing mechanism 4.

[0051] The above solution achieves micron-level positioning accuracy while maintaining a compact structure through a combination of synchronous belt drive and ball screw drive. Compared with gear and rack drive, this design avoids backlash error and reduces operating noise.

[0052] The above technical solution achieves precise vertical positioning of the dispensing mechanism 4, solving the problem of sample placement deviation caused by mechanical transmission errors in traditional equipment. The use of a lubrication-free synchronous belt drive combined with a pre-tensioned ball screw ensures transmission accuracy while reducing maintenance requirements and extending the replacement cycle of consumables.

[0053] like Figures 1 to 6 As shown, in some embodiments of the present invention, the mounting bracket 102 is further provided with a guide plate 103, and one end of the lead screw A 305 away from the driven wheel B 304 is provided on the guide plate 103. The guide plate 103 is a support structure mounted on the mounting bracket 102, used to provide axial positioning support for the lead screw and prevent radial offset. Specifically, the end of the lead screw A 305 away from the driven wheel B 304 is located on the guide plate 103, meaning that the end of the lead screw is mounted in the positioning hole of the guide plate 103 through a bearing or bushing structure. This can be achieved by interference fit or flange connection. This structural design can effectively constrain the axial movement trajectory of the lead screw.

[0054] The guide plate 103 engages with the end of the lead screw A 305 to further constrain the radial runout of the lead screw A 305. For example, a guide structure containing linear bearings can be used to ensure smooth and reliable movement.

[0055] In addition, a linear guide rail D 308 is provided on the inner side of the mounting bracket 102, and the up-and-down moving slider 307 of the sampling gun is slidably mounted on the linear guide rail D 308; the setting of the linear guide rail D 308 can further constrain the position of the up-and-down moving slider 307 of the sampling gun, so as to prevent it from deviating during the up-and-down movement. like Figures 1 to 6As shown, in some embodiments of the present invention, the liquid dispensing mechanism 4 includes a top plate B 400, a stepper motor C 402, a synchronous belt C 403, a driving wheel C 404, a driven wheel C 405, and a lead screw B 406. The top plate B 400 is disposed on the movable connecting seat 401, and the synchronous belt C 403 is wound around the driving wheel C 404 and the driven wheel C 405. Stepper motor C 402 is located at the bottom of top plate B 400, while drive wheel C 404 and driven wheel C 405 are located at the top of top plate B 400. The output end of the stepper motor C 402 passes through the top plate B 400 and is connected to the drive wheel C 404; The lead screw B 406 passes through the top plate B 400 and is connected to the driven wheel C 405; A lead screw slider B 407 is sleeved on the outside of the lead screw B 406, and a sampling gun slider 408 is provided on the lead screw slider B 407; A fixing seat 409 is sleeved on the outside of the sampling gun body 414, and the fixing seat 409 is located on the sampling gun sliding block 408; The top plate B 400 is a support structure for carrying the stepper motor C 402 and transmission components. It can be made of sheet metal to ensure the installation accuracy of the transmission system. The stepper motor C 402 is the power source that drives the sampling gun; it can be a two-phase hybrid stepper motor for micro-step control. The synchronous belt C 403 is the transmission component connecting the driving pulley C 404 and the driven pulley C 405; it can be a synchronous belt made of polyurethane material for precise transmission of rotational motion. The lead screw B 406 is the mechanical element that converts rotational motion into linear motion; it can be a ball screw, working in conjunction with the lead screw slider B 407 to achieve linear displacement of the sampling gun. The sampling gun slider 408 is the moving component connected to the lead screw slider B 407; it can be made of aluminum alloy with a self-lubricating coating on its sliding surface to reduce motion resistance.

[0056] Specifically, the movable connecting seat 401 is mounted on the moving mechanism 3, and specifically, the movable connecting seat 401 is installed on the up-and-down moving slider 307 of the sampling gun; the top plate B 400 is fixed to the top of the movable connecting seat 401 by bolts. After the stepper motor C 402 is powered on, it drives the driving wheel C 404 to rotate, and transmits the power to the driven wheel C 405 through the synchronous belt C 403, driving the lead screw B 406 to rotate synchronously. The rotational motion of the lead screw B 406 is converted into linear displacement by the lead screw slider B 407, which drives the sampling gun sliding block 408 to move along the height direction of the movable connecting seat 401. The fixed seat 409 is rigidly connected to the sampling gun sliding block 408, so that the button on the sampling gun body 414 can move synchronously with the sliding block to complete the liquid aspiration or drainage operation. During the movement, the step angle of the stepper motor C 402 can be precisely controlled by the controller to achieve precise positioning of the sampling gun in the vertical direction.

[0057] More specifically, the housing of the sampling gun body 414 can be mounted on the movable connecting seat 401. When the stepper motor C 402 is started, it drives the lead screw B 406 to rotate synchronously via the synchronous belt C 403, so that the button on the sampling gun body 414 can move synchronously with the sampling gun sliding block 408 to complete the liquid aspiration or drainage operation.

[0058] In summary, the liquid dispensing mechanism 4 achieves high-precision vertical positioning of the sampling gun, meeting the accuracy requirements for micro-volume liquid transfer. The modular design reduces the overall size of the dispensing mechanism 4, facilitating integration into portable testing equipment. The digital control characteristics of the stepper motor support communication with a host computer, enabling fully automated liquid transfer operations; operators only need to input parameters to complete the testing process.

[0059] like Figures 1 to 6 As shown, in some embodiments of the present invention, the movable connecting seat 401 is provided with a linear guide rail B410, and the sampling gun sliding block 408 is slidably disposed on the linear guide rail B410; that is, the linear guide rail B410 disposed on the movable connecting seat 401 and the sampling gun sliding block 408 form a sliding pair, further ensuring the straightness of the moving trajectory.

[0060] like Figures 1 to 6 As shown, in some embodiments of the present invention, the fixing base 409 is further provided with a connector 413, the connector 413 is provided with a TIP head push plate 411, and the TIP head push plate 411 is provided with an opening block 412. The TIP head push plate 411 has a mounting hole that matches the sampling gun body 414. Specifically, one end of the sampling gun body 414 for mounting the TIP head (sampling end) is set in the mounting hole, and the sampling end is adapted to the mounting hole. The connector 413 refers to the connection structure used to connect the TIP head push plate 411 and the fixed base 409.

[0061] The opening block 412 refers to the structure set on the TIP head push plate 411. Specifically, it can adopt a modular design with a guide bevel and can be used for opening operations. The mounting hole refers to the positioning hole that matches the outer diameter of the sampling end (i.e., the end where the TIP head is mounted) of the sampling gun body 414.

[0062] like Figures 1 to 6 As shown, in some embodiments of the present invention, a sliding hole is provided on the fixing base 409 to cooperate with the connector 413, and the connector 413 is slidably disposed in the sliding hole; The fixed base 409 is also provided with a driving component, which is used to drive the connecting component 413 to rise and fall. The driving component can be a cylinder. When the sampling gun needs to retract the TIP head, the driving component drives the connector 413 to descend, that is, the TIP head push plate 411 on the connector 413 descends. Since the outer diameter of the TIP head on the sampling gun body 414 is larger than the outer diameter of the sampling end on the sampling gun body 414, the TIP head is restricted from rising with the sampling gun body 414 due to the obstruction of the TIP head push plate 411, thereby causing the TIP head to detach from the sampling gun body 414 and completing the TIP head retraction operation.

[0063] This structure automates the TIP head replacement process, effectively reducing the frequency of manual intervention.

[0064] like Figures 1 to 6 As shown, in some embodiments of the present invention, the driving mechanism includes a detection mechanism fixing plate 504, a stepper motor D 505, a first cam 506, a second cam 507, a needle sliding block 508, and a linear guide rail C 509. One side of the needle sliding block 508 is slidably mounted on the linear guide rail C 509, and the other side of the needle sliding block 508 is connected to the needle fixing plate 501. The linear guide rail C 509 is vertically oriented. Stepper motor D 505 is located on one side of the detection mechanism fixing plate 504, and linear guide rail C 509 is located on the other side of the detection mechanism fixing plate 504; The output end of the stepper motor D505 passes through the detection mechanism fixing plate 504 and is connected to the first cam 506; The end of the first cam 506 is connected to the second cam 507, and the needle sliding block 508 is provided with a straight groove 510 that cooperates with the second cam 507. The detection mechanism fixing plate 504 is a support structure for mounting the drive mechanism. It can be made of sheet metal and supports the stepper motor D 505 and the linear guide rail C 509. The stepper motor D 505 is the power element that drives the cam rotation; it can be a stepper motor with high-precision position control capabilities, used to convert electrical signals into mechanical rotational motion. The first cam 506 and the second cam 507 are transmission elements that convert rotational motion into linear motion; they can be eccentric wheel structures, achieving displacement output through changes in the cam profile. The needle slide block 508 is a moving part that cooperates with the linear guide rail C509; it can be made of aluminum alloy, and its side linear groove 510 constrains the movement trajectory of the second cam 507, thus converting cam rotation into linear displacement. The linear guide rail C509 is a guide structure that guides the movement of the needle slide block 508; it can be a ball-bearing linear guide rail, used to ensure the movement accuracy of the needle fixing plate 501.

[0065] Specifically, after the stepper motor D 505 starts, it drives the first cam 506 to rotate. The first cam 506 is linked with the second cam 507, which is embedded in the linear groove 510 of the needle slide block 508. When the second cam 507 rotates with the stepper motor D 505, the contact point between its contour and the linear groove 510 changes periodically, forcing the needle slide block 508 to reciprocate linearly along the direction of the linear guide rail C 509. The movement of the needle slide block 508 directly drives the needle fixing plate 501 to move synchronously, thereby controlling the lifting and lowering of the liquid outlet needle 502 and the liquid inlet needle 503. For example, when the second cam 507 is at its maximum lift position, the needle fixing plate 501 drives the detection needle to be fully inserted into the reaction hole; when the cam rotates to the lowest position, the detection needle is raised to a safe height. During this process, the linear guide rail C 509 constrains the movement path of the needle slide block 508 to prevent the needle body from colliding due to movement deviation.

[0066] Compared to existing technologies, traditional probe drive mechanisms often use pneumatic or hydraulic cylinders for lifting, requiring complex pneumatic or hydraulic systems, resulting in bulky equipment and high maintenance costs. This solution, however, directly converts rotary motion into linear motion through a combination of a cam mechanism and a stepper motor, eliminating the need for an external pneumatic or hydraulic pressure source and simplifying the overall structure. Furthermore, the interaction between the cam profile and the linear slide 510 allows for precise control of the probe's stroke and speed, avoiding the impact problems inherent in traditional electromagnetic drives.

[0067] Through the above technical solution, this application achieves precise positioning and stable driving of the detection needle assembly 5, solving the problems of complex liquid circuits and large size in existing electrochemical analysis instruments. After the cam transmission mechanism replaces the traditional gas-liquid drive unit, there is no need to arrange complex pipelines inside the device, reducing the amount of consumables used. At the same time, the compact structural design makes the instrument more suitable for the rapid detection needs in POCT scenarios.

[0068] like Figures 1 to 6 As shown, in some embodiments of the present invention, the liquid circuit module is equipped with a peristaltic pump 6. The peristaltic pump 6 refers to a device that achieves liquid transfer by squeezing a flexible tube with a rotating roller. Specifically, it can be implemented using a peristaltic pump 6 with an adjustable speed motor, and the liquid flow rate is controlled by adjusting the rotation speed. The liquid circuit module refers to the pipeline system connecting the liquid outlet needle 502, the liquid inlet needle 503, and the detection unit 7. Specifically, it can adopt a combination structure of silicone flexible tube and connector to construct a closed liquid transfer channel.

[0069] Specifically, the peristaltic pump 6 is integrated into the fluid circuit module, with its hoses connected to the outlet needle 502 and the inlet needle 503. When the detection unit 7 is activated, the rollers of the peristaltic pump 6 rotate at a preset speed, squeezing the hose to generate negative pressure, drawing the liquid from the reaction orifice into the detection unit 7 through the inlet needle 503. After detection, the peristaltic pump 6 rotates in the reverse direction, driving the residual liquid back to the waste container. This design replaces traditional multi-valve control with mechanical squeezing, reducing the number of pipeline connection points.

[0070] A second aspect of the present invention also provides a detection method for a POCT detection device for nutrient detection, the method comprising the following steps: S1. Activation and cleaning of the detection unit The reagent kit is placed in the reagent kit holder 201, and the reagent kit holder 201 is moved along the length of the base plate 101 by the translation component 2 to position the activation solution tank in the reagent kit below the solution preparation mechanism 4; specifically, in the initial process, the activation solution tank on the reagent kit 208 is moved below the detection needle assembly 5 by the translation component 2. Driven by the drive mechanism on the detection needle assembly 5, the drive needle fixing plate 501 is lowered, causing the dispensing needle 502 and the inlet needle 503 to be inserted into the activation solution tank on the reagent kit; then the peristaltic pump 6 is started to deliver the activation solution into the detection unit 7, the dispensing needle 502 and the inlet needle 503 are raised, and the detection unit 7 begins to activate. After the detection unit 7 is activated, the reagent kit holder 201 continues to move to the cleaning solution tank position on the reagent kit 208. Through the drive mechanism on the detection needle assembly 5, the drive needle fixing plate 501 is lowered, which drives the liquid outlet needle 502 and the liquid inlet needle 503 downward, causing the peristaltic pump 6 to start and deliver the cleaning solution into the detection kit 7. The liquid outlet needle 502 and the liquid inlet needle 503 are raised, and the detection unit 7 begins to be cleaned. S2, Extraction of reaction solution After cleaning, the TIP position on the reagent kit 208 moves to the bottom of the sampling gun body 414. The moving mechanism 3 drives the sampling gun body 414 on the liquid preparation mechanism 4 to extract the TIP head, and then lifts it up. The reagent kit 208 continues to move, so that the sample slot on it is below the sampling gun body 414. The moving mechanism 3 drives the liquid preparation mechanism 4 to descend. The TIP head on the sampling gun body 414 is inserted into the sample slot. The stepper motor on the liquid preparation mechanism 4 is started to extract the sample from the sample slot. After a certain amount of sample is extracted, the moving mechanism 3 drives the liquid preparation mechanism 4 to lift up. The bottom liquid well on reagent kit 208 moves to the sampling gun body 414. The moving mechanism 3 drives the liquid preparation mechanism 4 to descend. The TIP head is inserted into the bottom liquid well. The stepper motor on the liquid preparation mechanism 4 is started to extract the liquid in the bottom liquid well. After a certain amount of sample is extracted, the moving mechanism 3 drives the liquid preparation mechanism 4 to lift up. The reaction well on reagent kit 208 moves to the sampling gun body 414. The moving mechanism 3 drives the liquid preparation mechanism 4 to descend. The TIP head is inserted into the reaction well. The stepper motor on the liquid preparation mechanism 4 starts and spits out the liquid in the TIP head. After completion, the moving mechanism 3 drives the liquid preparation mechanism 4 to lift up. S3, Detection The reaction well on reagent kit 208 is moved under the liquid injection needle 503. The liquid injection needle 503 descends and is inserted into the reaction well. The peristaltic pump 6 is activated to deliver the liquid in the reaction well to the detection unit 7. The liquid injection needle 503 is lifted, and the detection unit 7 begins to work. S4, Reset The bottom liquid well on reagent kit 208 moves to the position below the injection needle 503. The injection needle 503 descends and inserts into the bottom liquid well. The peristaltic pump 6 starts, delivering the liquid in the bottom liquid well to the detection unit 7. The injection needle 503 then rises. The reagent kit moves to the starting position, completing the detection.

[0071] While the present invention has been disclosed above, its scope of protection is not limited thereto. Those skilled in the art can make various changes and modifications without departing from the spirit and scope of the present invention, and all such changes and modifications will fall within the scope of protection of the present invention.

Claims

1. A POCT (Point-of-Care Testing) device for nutrient detection, characterized in that, include: The support member (1) includes a base plate (101) and a mounting bracket (102) disposed on the base plate (101). Translation component (2), the translation component (2) includes a reagent kit holder (201) and a driving component, the driving component is disposed on the base plate (101), the driving component is used to drive the reagent kit holder (201) to move along the length direction of the base plate (101); The liquid preparation mechanism (4) includes a movable connecting seat (401) and a sampling gun body (414) disposed on the movable connecting seat (401). The moving mechanism (3) is mounted on the mounting frame (102) and is used to drive the liquid dispensing mechanism (4) to move along the height direction of the mounting frame (102); The detection needle assembly (5) includes a drive mechanism and a needle fixing plate (501). The drive mechanism is mounted on the mounting bracket (102) and is used to drive the needle fixing plate (501) to move. The needle fixing plate (501) is provided with an outlet needle (502) and an inlet needle (503). The detection unit (7) is connected to the liquid outlet needle (502) and the liquid inlet needle (503) respectively through the liquid circuit module.

2. The POCT detection device for nutrient detection according to claim 1, characterized in that, It also includes a control component (9), which is connected to the translation component (2), the liquid dispensing mechanism (4), the moving mechanism (3), the detection needle component (5), and the detection unit (7), respectively.

3. The POCT detection device for nutrient detection according to claim 1, characterized in that, The drive assembly includes a linear guide rail A (202), a stepper motor A (203), a drive wheel A (204), a synchronous belt A (205), a driven wheel A (206), and a driven frame (207). The driven frame (207), the stepper motor A (203), and the linear guide rail A (202) are all mounted on the base plate (101). The driving wheel A (204) is located at the output end of the stepper motor A (203), the driven wheel A (206) is located on the driven frame (207), and the synchronous belt A (205) is wound around the driving wheel A (204) and the driven wheel A (206); The reagent kit holder (201) has a connecting block on its side, and the connecting block is located on the synchronous belt A (205); The reagent kit holder (201) is slidably mounted on the linear guide rail A (202).

4. The POCT detection device for nutrient detection according to claim 1, characterized in that, The moving mechanism (3) includes a top plate A (300), a stepper motor B (301), a timing belt B (302), a drive wheel B (303), a driven wheel B (304), and a lead screw A (305). The top plate A (300) is located on the mounting bracket (102) on the side away from the bottom plate (101), and the timing belt B (302) is wound around the drive wheel B (303) and the driven wheel B (304). The stepper motor B (301) is mounted on the top plate A (300), and the driving wheel B (303) and the driven wheel B (304) are both mounted on the top plate A (300) on the side away from the stepper motor B (301). The output end of the stepper motor B (301) passes through the top plate A (300) and is connected to the drive wheel B (303); The lead screw A (305) passes through the top plate A (300) and is connected to the driven wheel B (304); The lead screw A (305) is fitted with a lead screw slider A (306), and the lead screw slider A (306) is provided with a sampling gun up-and-down moving slider (307). The movable connecting seat (401) is provided on the sampling gun up-and-down moving slider (307).

5. The POCT detection device for nutrient detection according to claim 4, characterized in that, The mounting bracket (102) is also provided with a guide plate (103), and one end of the lead screw A (305) away from the driven wheel B (304) is provided on the guide plate (103).

6. The POCT detection device for nutrient detection according to claim 1, characterized in that, The liquid dispensing mechanism (4) includes a top plate B (400), a stepper motor C (402), a synchronous belt C (403), a drive wheel C (404), a driven wheel C (405), and a lead screw B (406). The top plate B (400) is mounted on the movable connecting seat (401), and the synchronous belt C (403) is wound around the drive wheel C (404) and the driven wheel C (405). The stepper motor C (402) is mounted on the top plate B (400), and the driving wheel C (404) and the driven wheel C (405) are both mounted on the top plate B (400) on the side away from the stepper motor C (402); The output end of the stepper motor C (402) passes through the top plate B (400) and is connected to the drive wheel C (404); The lead screw B (406) passes through the top plate B (400) and is connected to the driven wheel C (405); The lead screw B (406) is fitted with a lead screw slider B (407), and the lead screw slider B (407) is provided with a sampling gun slider block (408). The sampling gun body (414) is fitted with a fixing seat (409), which is located on the sampling gun sliding block (408).

7. The POCT detection device for nutrient detection according to claim 6, characterized in that, The movable connecting seat (401) is provided with a linear guide rail B (410), and the sampling gun sliding block (408) is slidably disposed on the linear guide rail B (410).

8. The POCT detection device for nutrient detection according to claim 6, characterized in that, The fixing base (409) is also provided with a connector (413), the connector (413) is provided with a TIP head push plate (411), and the TIP head push plate (411) is provided with an opening block (412). The TIP head push plate (411) has mounting holes that cooperate with the sampling gun body (414).

9. The POCT detection device for nutrient detection according to claim 1, characterized in that, The driving mechanism includes a detection mechanism fixing plate (504), a stepper motor D (505), a first cam (506), a second cam (507), a needle sliding block (508), and a linear guide rail C (509). The needle sliding block (508) is slidably mounted on the linear guide rail C (509) on one side, and the other side of the needle sliding block (508) is connected to the needle fixing plate (501). The stepper motor D (505) is located on one side of the detection mechanism fixing plate (504), and the linear guide rail C (509) is located on the other side of the detection mechanism fixing plate (504); The output end of the stepper motor D (505) passes through the detection mechanism fixing plate (504) and is connected to the first cam (506). The end of the first cam (506) is connected to the second cam (507), and the needle slide block (508) is provided with a linear slide groove (510) that cooperates with the second cam (507).

10. A detection method for a POCT detection device for nutrient detection according to any one of claims 1-9, characterized in that, The method includes the following steps: S1, Activation of the detection unit Place the reagent kit into the reagent kit holder (201), and drive the reagent kit holder (201) to move along the length of the base plate (101) through the translation component (2). Position the activation solution tank in the reagent kit below the solution preparation mechanism (4). Drive the dispensing needle (502) and the inlet needle (503) on the detection needle assembly (5) to insert them into the activation solution tank on the reagent kit. Then, deliver the activation solution into the detection unit (7). The dispensing needle (502) and the inlet needle (503) are lifted, and the detection unit (7) begins to activate. S2, Cleaning of the detection unit After the detection unit (7) is activated, the reagent kit holder (201) continues to move to the cleaning solution tank position on the reagent kit. Through the drive mechanism on the detection needle assembly (5), the drive needle fixing plate (501) is lowered, which drives the liquid outlet needle (502) and liquid inlet needle (503) downward to deliver the cleaning solution into the detection kit (7). The liquid outlet needle (502) and liquid inlet needle (503) are raised, and the detection unit (7) begins to clean. S3, Extraction of reaction solution After cleaning, the TIP position on the reagent kit moves to the bottom of the sampling gun body (414). The moving mechanism (3) drives the sampling gun body (414) on the liquid preparation mechanism (4) to extract the TIP head. After completion, it is lifted up. The reagent kit continues to move so that the sample slot on it is below the sampling gun body (414). The moving mechanism (3) drives the liquid preparation mechanism (4) to descend. The TIP head on the sampling gun body (414) is inserted into the sample slot to extract the sample from the sample slot. After extraction, the moving mechanism (3) drives the liquid preparation mechanism (4) to lift up. The bottom liquid well on the reagent kit is moved to the bottom of the sampling gun body (414). The moving mechanism (3) drives the liquid preparation mechanism (4) to descend. The TIP head is inserted into the bottom liquid well to extract the liquid in the bottom liquid well. After extraction, the moving mechanism (3) drives the liquid preparation mechanism (4) to rise. The reaction well on the reagent kit is moved to the bottom of the sampling gun body (414). The moving mechanism (3) drives the liquid preparation mechanism (4) to descend. The TIP head is inserted into the reaction well and the liquid in the TIP head is ejected. After completion, the moving mechanism (3) drives the liquid preparation mechanism (4) to rise. S4, Detection The reaction well on the kit is moved under the injection needle (503), the injection needle (503) descends and is inserted into the reaction well, and the liquid in the reaction well is delivered to the detection unit (7). The injection needle (503) is lifted up and the detection unit (7) starts to work. S5, Reset The bottom liquid well on the reagent kit is moved to the bottom of the injection needle (503). The injection needle (503) descends and inserts into the bottom liquid well, delivering the liquid in the bottom liquid well to the detection unit (7). The injection needle (503) is then lifted. The reagent kit moves to the starting position to complete the test.

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