RNA (Ribonucleic Acid) detection kit and use method thereof

Abstract

The invention provides an RNA (Ribonucleic Acid) detection kit and a use method, and relates to the field of detection kits. The RNA detection kit comprises a horizontal supporting base, two lateral grooves are formed in the two sides of the horizontal supporting base and distributed front and back, the inner surface of the lateral groove in the front side is fixedly connected with a second wear-resistant elastic piece, and the inner surface of the lateral groove in the rear side is fixedly connected with a second wear-resistant elastic piece. And the inner surface of the lateral groove on the rear side is fixedly connected with a lateral fixing rod. Through an inverted-cone-shaped leakage-proof piece and a water absorption cotton piece which can ascend and descend, the height of the inverted-cone-shaped leakage-proof piece can be adjusted according to needs in the using process, liquid in the inverted-cone-shaped leakage-proof piece is prevented from being scattered, stirring is achieved through a stirring device, shaking is avoided, and through a lateral groove, a lateral fixing rod and a bottom plastic connecting rod, the leakage-proof effect is achieved. The horizontal supporting bases can be arranged, and the use convenience is improved.

Description

Technical Field

[0001] This invention relates to the field of detection kit technology, specifically to an RNA detection kit and its usage method. Background Technology

[0002] RNA detection kits are chemical reagent kits used to detect RNA molecules. They typically include steps such as RNA extraction, transcription of RNA into cDNA, cDNA amplification, and detection of the amplified products. RNA detection kits can be used in many applications, such as gene expression detection, viral infection, and tumor diagnosis and treatment. Common RNA detection kits include reverse transcription kits, PCR amplification kits, and real-time quantitative PCR kits. These kits usually consist of various reagents, such as reverse transcriptase, polymerase, primers, probes, and buffers. When using RNA detection kits, it is essential to strictly follow the instructions to ensure accurate and reliable results.

[0003] However, one of the main drawbacks of RNA testing kits is the risk of false positives and false negatives. This can be due to technical issues in steps such as RNA sample extraction, reverse transcription, and PCR amplification, or problems with the kit itself. Kit-related issues refer to potential quality problems with certain reagents or components, or deficiencies or defects in the kit's design and formulation, leading to poor overall performance or erroneous test results. Additionally, insufficient or excessive reagent usage can cause spillage, resulting in poor detection results, or easily lead to contamination. Summary of the Invention

[0004] (a) Technical problems to be solved To address the shortcomings of existing technologies, this invention provides an RNA detection kit and its usage method, solving the problem of easy leakage and subsequent contamination.

[0005] (II) Technical Solution To achieve the above objectives, the present invention provides the following technical solution: an RNA detection kit, comprising: A horizontal support base has two lateral grooves on both sides, arranged front to back. A second wear-resistant elastic sheet is fixedly connected to the inner surface of the front lateral groove, and a lateral fixing rod is fixedly connected to the inner surface of the rear lateral groove. A first wear-resistant elastic sheet is fixedly connected to the outer surface of the lateral fixing rod. A bottom plastic connecting rod is fixedly connected to the inner wall of the bottom of the horizontal support base. Multiple horizontal support bases are arranged front to back, and the bottom plastic connecting rods extend into the interior of multiple horizontal support bases. The interior of the horizontal support base forms an arc-shaped cavity, and a sunken conical cavity is provided on the lower surface of the arc-shaped cavity. A first lateral fixing sleeve is fixedly connected to the upper surface of the horizontal support base. A second lateral fixing sleeve is slidably connected to the outer surface of the first lateral fixing sleeve. A leak-proof washer is fixedly connected to the inner surface of the second lateral fixing sleeve. The inner surface of the leak-proof washer contacts the outer surface of the first lateral fixing sleeve. An inverted conical leak-proof sheet is fixedly connected to the inner surface of the second lateral fixing sleeve. A horizontal fixing plate is fixedly connected to the outer surface of the second lateral fixing sleeve. A cutting line is provided on the upper surface of the horizontal fixing plate. An absorbent cotton sheet is fixedly connected to the upper surface of the horizontal fixing plate. A stirring device, comprising a first horizontal fixed plate, a spiral plate, a second fixed plate, a connecting shaft, a stirring rod, and a stirring device, wherein the first horizontal fixed plate is fixedly connected to the inner surface of an inverted conical leak-proof plate, the lower surface of the first horizontal fixed plate is rotatably connected to the spiral plate, the lower surface of the spiral plate is fixedly connected to the connecting shaft, the lower surface of the connecting shaft is fixedly connected to the stirring rod, and the second fixed plate is fixedly connected to the inner surface of a first lateral fixed sleeve.

[0006] Preferably, there are two second wear-resistant elastic sheets and two first wear-resistant elastic sheets, which are distributed vertically. The distance between the two first wear-resistant elastic sheets is 1.1 times the distance between the two second wear-resistant elastic sheets, and the elasticity range of the first wear-resistant elastic sheets and the second wear-resistant elastic sheets is within the range of the elasticity of the first wear-resistant elastic sheets and the second wear-resistant elastic sheets, so as to facilitate the connection of the device.

[0007] Preferably, the lower surface of the inverted conical leak-proof sheet is provided with a circular hole, the diameter of which is half the diameter of the inverted conical leak-proof sheet, and the number of leak-proof gaskets is two, which are distributed vertically to increase the sealing performance between the first lateral fixing sleeve and the second lateral fixing sleeve.

[0008] Preferably, the inner surface of the absorbent cotton pad is fixedly connected to the second lateral fixing sleeve to prevent liquid outside the second lateral fixing sleeve from flowing down and causing pollution.

[0009] Preferably, the upper surface of the second fixing plate is provided with a through hole, which is slidably connected to the spiral plate and is used to drive the stirring rod to rotate by the lifting and lowering of the first horizontal fixing plate.

[0010] Preferably, the lower side of the stirring rod extends into the interior of the arc-shaped cavity.

[0011] Preferably, the second lateral fixing sleeve and the first lateral fixing sleeve are made of transparent material.

[0012] A method for using an RNA detection kit includes the following steps: a. As needed, multiple horizontal support bases can be connected to the side fixing rods via side grooves, or the horizontal support bases can be separated by breaking off the bottom plastic connecting rod along its axis to achieve the desired arrangement. b. Check that all reagents are within their expiration date and check for any leaks; c. If the reagents are qualified, place all the required reagents inside the arc-shaped cavity, and adjust the height of the inverted conical anti-leakage plate according to the amount of reagents, that is, pull it up and down, so as to prevent the liquid with a high liquid level in the first lateral fixed sleeve from spilling out. d. If spillage occurs, first absorb it with absorbent cotton pads; e. During the rising and falling of the inverted conical leak-proof plate, the spiral plate rotates, which drives the stirring rod to rotate and stir the liquid, avoiding the need for shaking and causing spillage; f. When it is necessary to remove liquid, place the test tube inside the recessed conical cavity to facilitate more thorough aspiration of the liquid.

[0013] (III) Beneficial Effects This invention provides an RNA detection kit and its usage method. It has the following beneficial effects: 1. The horizontal support base, with its adjustable inverted cone-shaped leak-proof sheet and absorbent cotton pad, allows for height adjustment of the inverted cone-shaped leak-proof sheet during use to prevent internal liquid from spilling. It also features a stirring device to prevent shaking.

[0014] 2. The horizontal support base can be arranged by means of lateral grooves, lateral fixing rods and bottom plastic connecting rods, increasing the convenience of use. Attached Figure Description

[0015] 1. Figure 1 This is a schematic diagram of the overall structure of the present invention; 2. Figure 2 This is an enlarged internal schematic diagram of a portion of the structure of the present invention; 3. Figure 3 for Figure 2 Enlarged schematic diagram of the structure at point A in the middle; 4. Figure 4 This is a schematic diagram of the horizontal support base structure of the present invention; 5. Figure 5 This is a schematic diagram of the internal structure of the second lateral fixing sleeve of the present invention.

[0016] The components include: 1. Horizontal support base; 2. Bottom plastic connecting rod; 3. Lateral groove; 4. Lateral fixing rod; 5. First wear-resistant elastic sheet; 6. Arc-shaped cavity; 7. Recessed conical cavity; 8. First lateral fixing sleeve; 9. Second wear-resistant elastic sheet; 10. Second lateral fixing sleeve; 11. Horizontal fixing plate; 12. Inverted conical leak-proof sheet; 13. Absorbent cotton sheet; 14. Round hole; 15. Leak-proof washer; 16. First horizontal fixing piece; 17. Spiral blade; 18. Second fixing piece; 19. Connecting shaft; 20. Stirring rod; 21. Stirring device; 22. Cutting line. Detailed Implementation

[0017] The technical solutions in the embodiments of the present invention have been clearly and completely described. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] This invention provides an RNA detection kit, comprising: A horizontal support base 1 has two lateral grooves 3 on both sides, arranged front to back. A second wear-resistant elastic sheet 9 is fixedly connected to the inner surface of the front lateral groove 3. Two first wear-resistant elastic sheets 5 and two second wear-resistant elastic sheets 5 are arranged vertically. The distance between the two first wear-resistant elastic sheets 5 is 1.1 times the distance between the two second wear-resistant elastic sheets 9, and the distance is within the elastic range of the first wear-resistant elastic sheets 5 and the second wear-resistant elastic sheets 9, facilitating installation. The connection is as follows: a lateral fixing rod 4 is fixedly connected to the inner surface of the rear lateral groove 3, and a first wear-resistant elastic sheet 5 is fixedly connected to the outer surface of the lateral fixing rod 4. A bottom plastic connecting rod 2 is fixedly connected to the bottom inner wall of the horizontal support base 1. There are multiple horizontal support bases 1 distributed front and back. The bottom plastic connecting rod 2 extends into the interior of multiple horizontal support bases 1 respectively. The interior of the horizontal support base 1 forms an arc-shaped cavity 6. A sunken conical cavity 7 is provided on the lower surface of the arc-shaped cavity 6 to facilitate the suction of liquid.

[0019] A first lateral fixing sleeve 8 is fixedly connected to the upper surface of the horizontal support base 1. A second lateral fixing sleeve 10 is slidably connected to the outer surface of the first lateral fixing sleeve 8. A leak-proof washer 15 is fixedly connected to the inner surface of the second lateral fixing sleeve 10, and the inner surface of the leak-proof washer 15 contacts the outer surface of the first lateral fixing sleeve 8. An inverted conical leak-proof plate 12 is fixedly connected to the inner surface of the second lateral fixing sleeve 10 to prevent liquid leakage. A circular hole 14 is provided on the lower surface of the inverted conical leak-proof plate 12, and the diameter of the circular hole 14 is half the diameter of the inverted conical leak-proof plate 12. The leak-proof washer 15... There are two of them, arranged vertically, to increase the sealing performance between the first lateral fixing sleeve 8 and the second lateral fixing sleeve 10. The second lateral fixing sleeve 10 and the first lateral fixing sleeve 8 are made of transparent material. A horizontal fixing plate 11 is fixedly connected to the outer surface of the second lateral fixing sleeve 10. A cutting line 22 is provided on the upper surface of the horizontal fixing plate 11. An absorbent cotton sheet 13 is fixedly connected to the upper surface of the horizontal fixing plate 11. The inner surface of the absorbent cotton sheet 13 is fixedly connected to the second lateral fixing sleeve 10 to prevent liquid from flowing down and causing pollution on the outside of the second lateral fixing sleeve 10.

[0020] The stirring device 21 includes a first horizontal fixing plate 16, a spiral plate 17, a second fixing plate 18, a connecting shaft 19, a stirring rod 20, and the stirring device 21. The upper surface of the second fixing plate 18 is provided with a through hole, which is slidably connected to the spiral plate 17. It is used to drive the stirring rod 20 to rotate by the lifting and lowering of the first horizontal fixing plate 16. The first horizontal fixing plate 16 is fixedly connected to the inner surface of the inverted conical leak-proof plate 12. The lower surface of the first horizontal fixing plate 16 is rotatably connected to the spiral plate 17. The lower surface of the spiral plate 17 is fixedly connected to the connecting shaft 19. The lower surface of the connecting shaft 19 is fixedly connected to the stirring rod 20. The lower side of the stirring rod 20 extends into the interior of the arc-shaped cavity 6. The second fixing plate 18 is fixedly connected to the inner surface of the first lateral fixing sleeve 8.

[0021] A method for using an RNA detection kit includes the following steps: a. Connect multiple horizontal support bases 1 to the lateral fixing rods 4 via the lateral grooves 3 as needed, or break the bottom plastic connecting rods 2 along their axis to separate the horizontal support bases 1 and obtain the required arrangement. b. Check that all reagents are within their shelf life and check for leaks. c. If the reagents are qualified, place all the required reagents inside the arc-shaped cavity 6. Adjust the height of the inverted conical leak-proof plate 12 according to the amount of reagents by pulling it up and down to prevent liquids with high liquid levels in the first lateral fixing sleeve 8 from spilling out. d. If spillage occurs, first absorb the liquid with absorbent cotton pads 13. e. During the rise and fall of the inverted conical leak-proof plate 12, the spiral plate 17 rotates, which drives the stirring rod 20 to rotate and stir the liquid, preventing spillage caused by shaking. f. When it is necessary to remove the liquid, place the test tube inside the sunken conical cavity 7 to facilitate more thorough liquid extraction.

[0022] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An RNA detection kit, characterized by, The utility model relates to a horizontal type support base (1) is provided with two lateral recesses (3) on both sides, the number of lateral recess (3) is two and is distributed in front and back, the inner surface of front lateral recess (3) is fixedly connected with second wear type elastic sheet (9), the inner surface of rear lateral recess (3) is fixedly connected with lateral fixed rod (4), the outer surface of lateral fixed rod (4) is fixedly connected with first wear type elastic sheet (5), the bottom inner wall of horizontal type support base (1) is fixedly connected with bottom plastic connecting rod (2), the number of horizontal type support base (1) is multiple and is distributed in front and back, bottom plastic connecting rod (2) extends to the inside of multiple horizontal type support base (1) respectively, the inside of horizontal type support base (1) forms arc cavity (6), the lower surface of arc cavity (6) is provided with sunken type conical cavity (7). The upper surface of horizontal type support base (1) is fixedly connected with first lateral fixed sleeve (8), the outer surface of first lateral fixed sleeve (8) is slidably connected with second lateral fixed sleeve (10), the inner surface of second lateral fixed sleeve (10) is fixedly connected with leakproof washer (15), the inner surface of leakproof washer (15) is in contact with the outer surface of first lateral fixed sleeve (8), the inner surface of second lateral fixed sleeve (10) is fixedly connected with inverted conical leakproof sheet (12), the outer surface of second lateral fixed sleeve (10) is fixedly connected with horizontal type fixed plate (11), the upper surface of horizontal type fixed plate (11) is provided with cutting line (22), the upper surface of horizontal type fixed plate (11) is fixedly connected with water-absorbing cotton sheet (13). The stirring device (21) comprises a first horizontal fixing sheet (16), a spiral sheet (17), a second fixing sheet (18), a connecting shaft (19), a stirring rod (20) and the stirring device (21), the inner surface of the first horizontal fixing sheet (16) and the inverted conical leakproof sheet (12) are fixedly connected, the lower surface of the first horizontal fixing sheet (16) is rotatably connected with the spiral sheet (17), the lower surface of the spiral sheet (17) is fixedly connected with the connecting shaft (19), the lower surface of the connecting shaft (19) is fixedly connected with the stirring rod (20), and the inner surface of the second fixing sheet (18) and the first lateral fixed sleeve (8) are fixedly connected. The number of second wear type elastic sheet (9) and first wear type elastic sheet (5) is two and is distributed in upper and lower, the distance between two first wear type elastic sheets (5) is 1.1 times the distance between two second wear type elastic sheets (9).

2. The RNA detection kit according to claim 1, characterized in that: The lower surface of inverted conical leakproof sheet (12) is provided with round hole (14), the diameter of round hole (14) is half the diameter of inverted conical leakproof sheet (12), and the number of leakproof washer (15) is two and is distributed in upper and lower.

3. The RNA detection kit according to claim 1, characterized in that: The inner surface of water-absorbing cotton sheet (13) is fixedly connected with second lateral fixed sleeve (10).

4. The RNA detection kit according to claim 1, characterized in that: ​ 5. The RNA detection kit according to claim 1, characterized in that: The upper surface of the second fixed sheet (18) is provided with a through hole, and the through hole is in sliding connection with the spiral sheet (17).

6. The RNA detection kit according to claim 1, characterized in that: The lower side of the stirring rod (20) extends to the inside of the circular arc cavity (6).

7. The RNA detection kit according to claim 1, characterized in that: The second lateral fixing sleeve (10) and the first lateral fixing sleeve (8) are made of transparent material.

8. A method of using an RNA detection kit, the method comprising: The method comprises the following steps: a. According to the needs of use, a plurality of horizontal support bases (1) are connected with the lateral fixing rod (4) through the lateral groove (3), or the horizontal support bases (1) are separated by breaking the bottom plastic connecting rod (2) along the axis direction of the bottom plastic connecting rod (2) according to the needs, so that the required arrangement is obtained; b. Check whether the various reagents are within the shelf life, and check whether there is air leakage; c. Under the condition that the reagents are qualified, various required reagents are placed in the circular arc cavity (6), the height of the inverted conical leakage-proof sheet (12) is adjusted according to the amount of the reagent, that is, the inverted conical leakage-proof sheet (12) is pulled up and down, so that the liquid with high liquid level in the first lateral fixing sleeve (8) is prevented from spilling; d. If spilling occurs, the water-absorbing cotton sheet (13) is used for adsorption; e. In the process of rising and falling of the inverted conical leakage-proof sheet (12), the spiral sheet (17) rotates, so that the stirring rod (20) is driven to rotate, the liquid is stirred, and spilling caused by shaking is avoided; f. When the liquid needs to be removed, the test tube is placed in the sunken conical cavity (7), so that the liquid can be sucked more thoroughly.

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