Cultivation method of ganoderma lucidum spore powder for enhancing body immunity

Abstract

The invention belongs to the technical field of artificial cultivation of medicinal fungi, and particularly relates to a ganoderma lucidum spore powder cultivation method capable of enhancing body immunity, which comprises the following steps: new strain cultivation, intelligent cultivation room construction cultivation, sterile cut-log matrix composite cultivation, directional spore collection and activity retention treatment. According to the invention, a molecular marker-assisted selection technology is adopted, strains with high content of beta-glucan and triterpenoids are accurately screened out from a wild ganoderma lucidum resource library, key gene markers related to synthesis of immune active components are determined through gene sequencing, and excellent strains are fused by using a protoplast fusion technology to cultivate a new strain. Therefore, the strain with high immune active component yield is quickly obtained, and the quality of the ganoderma lucidum spore powder and the effect of enhancing immunity are guaranteed from the source; by building the intelligent cultivation room, the most suitable environmental condition is provided for the growth of the lucid ganoderma, so that the growth of the lucid ganoderma and the synthesis and accumulation of immunocompetence components are promoted.

Description

Technical Field

[0001] This invention belongs to the field of artificial cultivation technology of medicinal fungi, specifically a method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity. Background Technology

[0002] Reishi mushroom, a traditional and precious Chinese medicinal herb, has a long history of medicinal use and exhibits significant pharmacological activities in many aspects, including enhancing immunity, anti-tumor activity, and anti-oxidation. Reishi spore powder, as the reproductive cells of Reishi, inherits most of its effective components, especially β-glucan and triterpenoids, which play a crucial role in enhancing the body's immune function.

[0003] However, existing methods for cultivating Ganoderma lucidum spores have many shortcomings: In terms of strain selection, traditional methods rely heavily on empirical screening, lacking precise molecular biology techniques. This makes it difficult to quickly and accurately screen strains that produce high levels of immunomodulatory components, resulting in inconsistent levels of active ingredients in Ganoderma lucidum spore powder and affecting its immune-enhancing effects. Regarding environmental control, conventional cultivation rooms cannot accurately simulate the natural growth environment of Ganoderma lucidum, failing to provide suitable light, temperature, humidity, and CO2 concentrations, which is detrimental to the growth of Ganoderma lucidum and the synthesis and accumulation of immunomodulatory components. In the spore collection stage, traditional methods are inefficient and introduce many impurities, affecting the purity and quality of the spore powder. Furthermore, Ganoderma lucidum spores have a double-layered, hard shell: the outer wall is chitinous, and the inner wall is cellulose. Traditional methods such as mechanical grinding and enzymatic hydrolysis easily destroy internal active substances or leave harmful residues, making it difficult to fully release the active ingredients within the spores and reducing the bioavailability of Ganoderma lucidum spore powder.

[0004] Therefore, the present invention provides a method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity. Summary of the Invention

[0005] In order to overcome the shortcomings of the prior art, at least one technical problem raised in the background art is solved.

[0006] The technical solution adopted by this invention to solve its technical problem is: a method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity, comprising the following steps: S1. Using molecular marker-assisted selection technology, strains with high β-glucan and triterpenoid content were screened from the wild Ganoderma lucidum resource bank. Key gene markers related to the synthesis of immune active components were identified by gene sequencing. Using protoplast fusion technology, the selected strains with excellent traits were fused to cultivate new strains. S2. Construct an intelligent cultivation room with adjustable light, temperature, humidity, and CO2 concentration to simulate the photoperiod changes of Ganoderma lucidum during its natural growth season, and inoculate the newly screened strains into the intelligent cultivation room for cultivation. S3. Select hardwoods such as oak as logs, drill holes in the surface of the logs, fill them with a special culture medium, and use double-layer bagging technology to transfer the new strains that have been cultivated in the intelligent cultivation room for a period of time to the logs for further cultivation. In the double-layer bagging technology, the inner layer is a breathable and antibacterial film, and the outer layer is a light-blocking bag; S4. A negative pressure guided collection device is used to collect spores during the spore ejection period of the new strain and automatically separate impurities; S5. The collected spores are alternately subjected to short-term intense light irradiation and dark treatment using pulsed photoactivation technology, and sterilization technology is combined with supercritical CO2 fluid cell disruption technology. By removing minute impurities through nano-filtration, Ganoderma lucidum spore powder that enhances the body's immunity is obtained.

[0007] A further improvement of the present invention is that step S1 specifically comprises: DNA was extracted from various strains in the wild Ganoderma lucidum resource bank, and PCR amplification was performed using specific primers for genes related to β-glucan and triterpenoid synthesis. Positive strains were screened based on the amplification results. When determining key gene markers through gene sequencing, high-throughput sequencing technology is used to perform whole-genome sequencing on the screened positive strains, and bioinformatics analysis is used to identify gene fragments closely related to the synthesis of immune active components as key gene markers. In protoplast fusion technology, protoplasts of selected superior strains are first prepared, and then polyethylene glycol is used as a fusion agent to promote protoplast fusion. After screening and identification, new strains with high production of immune active components are obtained.

[0008] A further improvement of the present invention is that, in step S2, the illumination control of the intelligent cultivation room is achieved by setting multiple independently controllable light sources; Temperature and humidity control is achieved by using temperature and humidity sensors to monitor environmental parameters in the cultivation room in real time, and by automatically adjusting the system through air conditioning and humidifiers / dehumidifiers to maintain the temperature in the cultivation room between 25-28℃ and the relative humidity between 85%-90%. CO2 concentration is controlled by monitoring with CO2 sensors and by using the ventilation system to control the amount of CO2 entering and leaving the system. When simulating changes in the light cycle, a timer is used to precisely control the switching between light and darkness. The change in the light cycle is reflected in the setting of 12 hours of light and 12 hours of darkness.

[0009] A further improvement of the present invention is that, in step S3: Before drilling, the logs are subjected to high-temperature steam sterilization. The conditions for high-temperature steam sterilization are: maintaining at 121℃ for 2-3 hours. The diameter of the holes drilled on the surface of the log is 3-5mm, the depth of the holes is 1 / 3-1 / 2 of the diameter of the log, and the spacing between the holes is 5-8cm. The specially formulated culture medium is composed of sawdust, wheat bran, and trace elements, with a mass ratio of sawdust, wheat bran, and trace elements of 70-80:15-25:1-5. The trace elements include at least one of zinc, iron, manganese, and copper.

[0010] A further improvement of the present invention is that the specially formulated culture medium is pretreated before filling: After mixing sawdust and wheat bran, add an appropriate amount of water to make the moisture content reach 60%-70%, and then carry out pile fermentation treatment. The fermentation time is 7-10 days, and the pile is turned over every 2-3 days during the fermentation process. Trace elements are added in solution form, and the solution concentration is precisely prepared according to the type and content of the element.

[0011] A further improvement of the present invention is that, in step S4: The negative pressure range of the negative pressure guided collection device is -500Pa to -1000Pa. The negative pressure guided collection device has a built-in electrostatic adsorption plate and a low temperature condensation system. Automatic separation of spores and impurities is achieved through airflow control. The voltage of the electrostatic adsorption plate is set to 5-10kV, the temperature of the low temperature condensation system is controlled at 0-5℃, and airflow control is achieved by adjusting the fan speed.

[0012] A further improvement of the present invention is that S4 further includes using a laser particle counter to monitor the spore ejection amount in real time, and intelligently adjusting the acquisition parameters according to the monitoring results; Intelligent adjustment of acquisition parameters includes adjusting the negative pressure, fan speed, and acquisition time according to changes in spore ejection volume.

[0013] A further improvement of the present invention is that the negative pressure guided collection device is also provided with an odor guiding module, which releases specific volatile organic compounds emitted by Ganoderma lucidum spores; The specific volatile organic compounds are the main volatile components released by Ganoderma lucidum spores during the natural spraying period. Their composition and proportion were determined by gas chromatography-mass spectrometry and then artificially simulated for formulation.

[0014] A further improvement of the present invention is that, in step S5: The short-term intense light irradiation of the pulsed photoactivation technology uses an LED light source, and the light intensity is set between 5000-10000 lux according to the characteristics of different batches of spores. The spore powder processed by supercritical CO2 fluid cell disruption technology needs to be dried. The drying method is vacuum freeze drying, with a drying temperature of -40℃ to -30℃. The drying time is determined according to the moisture content of the spore powder, which is less than 5%. The sterilization technology uses purified water wet heat sterilization combined with ultraviolet synergistic treatment.

[0015] A further improvement of the present invention is that, in the pulsed photoactivation technology, a pulsed magnetic field of a specific wavelength is introduced during the alternation of short-duration intense light irradiation and dark treatment, which works synergistically with the photoactivation process; The magnetic field pulse frequency is 10-50 Hz, and the magnetic field strength is 10-50 mT.

[0016] The beneficial effects of this invention are as follows: 1. This invention employs molecular marker-assisted selection technology to precisely screen strains with high β-glucan and triterpenoid content from wild Ganoderma lucidum resource banks. Key gene markers related to the synthesis of immunomodulatory components are identified through gene sequencing. Furthermore, protoplast fusion technology is used to fuse superior strains to cultivate new strains, significantly improving screening efficiency and accuracy. This allows for the rapid acquisition of strains that produce high levels of immunomodulatory components, ensuring the quality of Ganoderma lucidum spore powder and its immune-enhancing effects from the source. An intelligent cultivation chamber with adjustable light, temperature, humidity, and CO2 concentration is constructed to precisely simulate the photoperiodic changes of Ganoderma lucidum during its natural growth season. Multiple independently controlled light sources, temperature and humidity sensors, CO2 sensors, and corresponding control equipment provide the most suitable environmental conditions for Ganoderma lucidum growth, thereby promoting its growth and the synthesis and accumulation of immunomodulatory components. 2. This invention employs a negative pressure guided collection device with a built-in electrostatic adsorption plate and a low-temperature condensation system. Automatic separation of spores from impurities is achieved through airflow control. Simultaneously, an odor-guiding module releases specific volatile organic compounds emitted by Ganoderma lucidum spores, guiding them into the collection device more efficiently. This improves collection efficiency and spore purity while reducing impurity contamination. Secondly, pulsed photoactivation technology is used to alternately subject the collected spores to short-duration intense light irradiation and dark treatment, while introducing a specific wavelength pulsed magnetic field for synergistic effect. This activates enzyme activity related to the synthesis of immune-active components within the spores, further enhancing the Ganoderma lucidum's spore purity. The efficacy of Ganoderma lucidum spore powder in enhancing the body's immunity: This invention utilizes supercritical CO2 fluid cell disruption technology combined with sterilization technology. Supercritical CO2 penetrates into the cell interior under high pressure. When the pressure is suddenly reduced, the CO2 rapidly vaporizes, causing a sharp increase in the pressure difference between the inside and outside of the cell. The cell wall ruptures due to violent expansion, while avoiding mechanical damage to intracellular components. In addition, CO2 has an extractive effect on lipids and can destroy the chemical structure of the cell wall, assisting in cell disruption. Compared with mechanical or chemical methods, supercritical CO2 cell disruption technology can more thoroughly release intracellular active substances, improve extraction efficiency, achieve good cell disruption effect, and ensure the hygiene, safety, activity, and stability of the spore powder. Attached Figure Description

[0017] The invention will now be further described with reference to the accompanying drawings.

[0018] Figure 1 This is a flowchart of the cultivation process of the present invention. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0020] Please see Figure 1 , This embodiment provides a method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity, including the following steps: S1. Cultivation of new strains: Multiple strains were selected from the wild Ganoderma lucidum resource bank, and DNA was extracted from each strain. PCR amplification was performed using specific primers for genes related to β-glucan and triterpenoid synthesis. Positive strains were screened based on the amplification results. High-throughput sequencing technology was used to perform whole-genome sequencing on the screened positive strains, and bioinformatics analysis was used to identify gene fragments closely related to the synthesis of immune active components as key gene markers. Protoplasts of the selected superior strains were prepared, and polyethylene glycol was used as a fusion agent to promote protoplast fusion. After screening and identification, new strains with high production of immunogenic components were obtained. It should be noted that the cultivation of new strains achieves strain optimization through gene-targeted screening and recombination technology: First, using PCR amplification technology, based on the principle of complementary DNA base pairing, specific primers for β-glucan and triterpenoid synthesis-related genes are designed to accurately screen positive strains carrying target genes; then, high-throughput sequencing combined with bioinformatics analysis such as comparison with NCBI and KEGG databases is used to locate key functional gene markers such as squalene synthase gene and β-glucan synthase gene; furthermore, protoplast fusion of different superior strains is induced by polyethylene glycol to achieve the integration and recombination of high-yield genes. This operation can accurately screen strains containing genes for the synthesis of highly active ingredients and enhance metabolic capacity through gene superposition effect, thereby overcoming the limitations of large differences in active ingredients and low screening efficiency of traditional wild Ganoderma lucidum strains. Through gene-targeted operation and cross-strain recombination, the targeted improvement process of high-yield strains is significantly accelerated, laying the genetic foundation for the subsequent cultivation of high-quality Ganoderma lucidum spore powder.

[0021] S2. Constructing an intelligent cultivation room for cultivation: A smart cultivation room with adjustable light, temperature, humidity, and CO2 concentration was built; multiple independently controllable light sources were set up, and the switching of light and darkness was precisely controlled by a timer to simulate the photoperiod changes of Ganoderma lucidum during its natural growth season, with 12 hours of light and 12 hours of darkness alternating. Temperature and humidity sensors are used to monitor environmental parameters in the cultivation room in real time. The system is automatically adjusted through air conditioning and humidifiers / dehumidifiers to maintain the temperature in the cultivation room between 25-28℃ and the relative humidity between 85%-90%. CO2 sensors are used to monitor the CO2 concentration in the cultivation room, and the amount of CO2 entering and leaving is controlled by the ventilation system to provide a suitable CO2 environment for the growth of Ganoderma lucidum. The newly screened strains were inoculated into a smart culture room for further cultivation. It should be noted that the intelligent cultivation chamber optimizes the growth conditions of Ganoderma lucidum through precise environmental control: on the one hand, it simulates the natural photocycle, activating circadian rhythm genes such as LgBC1 in Ganoderma lucidum with a 12-hour light / dark cycle to induce spore formation; on the other hand, it maintains a constant temperature of 25-28℃, humidity of 85%-90%, and dynamic CO2 concentration control through an intelligent system, providing optimal conditions for enzyme activity and optimizing photosynthetic efficiency. These regulatory measures not only promote the synthesis and metabolism of immune-active components such as triterpenoids, but also shorten the growth cycle from 120 days in the natural environment to 90 days, thereby increasing the triterpenoid content; given that fluctuations in natural climate can easily lead to instability in metabolic pathways, the intelligent cultivation chamber ensures the stability of growth conditions through real-time monitoring and automatic adjustment. Furthermore, sterols, such as ergosterol, in Ganoderma lucidum spores are one of the core components of Ganoderma lucidum's medicinal efficacy. They have the ability to enhance immune regulation, antioxidation, and anti-inflammation. Under prolonged mild light conditions, the sterols inside the spores will be fully activated. This activation process may involve structural changes in the sterols or synergistic effects with other active ingredients, thereby enhancing their biological activity. By further optimizing the light conditions in the intelligent cultivation room based on the simulation of natural photoperiods, a more favorable environment for the activation of sterols can be provided, which in turn helps to improve the efficacy of the final cultivated Ganoderma lucidum spore powder.

[0022] S3, Aseptic log substrate composite culture: Hardwoods such as oak are selected as logs. Before drilling, the logs are sterilized by high-temperature steam and kept at 121℃ for 2-3 hours. Drill holes on the surface of the log, with a diameter of 3-5mm and a depth of 1 / 3-1 / 2 of the log diameter, and a spacing of 5-8cm between the holes; To prepare a special culture medium, sawdust and wheat bran are mixed at a mass ratio of 70-80:15-25, and an appropriate amount of water is added to achieve a moisture content of 60%-70%. The mixture is then composted and fermented for 7-10 days, with the pile turned over every 2-3 days during fermentation. Trace elements, including at least one of zinc, iron, manganese, and copper, are added in solution form. The solution concentration is precisely prepared according to the type and content of each element. The trace element solution is mixed with the fermented sawdust and wheat bran to obtain a special culture medium with a mass ratio of sawdust, wheat bran, and trace elements of 70-80:15-25:1-5. The specially prepared culture medium is filled into the drilled holes in the logs. A double-layer bagging technique is used, with an inner layer of breathable and antibacterial film and an outer layer of light-blocking bag. The new strains that have been cultivated in the intelligent cultivation room for a period of time are transferred to the logs for further cultivation. It should be noted that the aseptic log substrate composite cultivation optimizes the microenvironment for Ganoderma lucidum growth through the synergistic effect of multiple processes: Firstly, it uses 121℃ high-temperature steam to deeply sterilize the logs, thoroughly eliminating unwanted microorganisms and nutrient competition. Secondly, it prepares a sawdust / wheat bran fermentation substrate to provide easily absorbed carbon and nitrogen sources, and activates the activity of key metabolic enzymes such as superoxide dismutase by adding trace elements such as zinc and iron. Thirdly, it employs a double-layer bagging technology—an inner antibacterial film to isolate contamination and an outer light-blocking bag to regulate the light environment—to construct a clean and stable microecology. This operation, through aseptic protection and nutrient enhancement, significantly improves mycelial biomass and the synthesis efficiency of active ingredients such as triterpenoids. Compared to the shortcomings of traditional open cultivation, which is susceptible to interference from unwanted microorganisms leading to metabolic instability, this closed cultivation ensures stable production of active ingredients through environmental control.

[0023] S4. Directed spore collection: A negative pressure guided collection device was used to collect spores during the spore ejection period of the new strain. The negative pressure range of the negative pressure guided collection device was -500Pa to -1000Pa. It had an integrated electrostatic adsorption plate and a low-temperature condensation system. The voltage of the electrostatic adsorption plate was set to 5-10kV, and the temperature of the low-temperature condensation system was controlled at 0-5℃. Airflow control was achieved by adjusting the fan speed, thus realizing the automatic separation of spores and impurities. An odor-guiding module is set up. After analyzing the composition and proportion of the main volatile organic compounds released by Ganoderma lucidum spores during the natural spraying period using gas chromatography-mass spectrometry, artificial simulation formulation is carried out to release the specific volatile organic compounds and guide the spores into the collection device more efficiently. A laser particle counter is used to monitor the spore ejection volume in real time, and the acquisition parameters are intelligently adjusted according to the monitoring results, including adjusting the negative pressure, fan speed and acquisition time according to the changes in spore ejection volume. It should be noted that the directional spore collection technology achieves precise collection through the synergistic effect of negative pressure guidance, odor chemotaxis, and intelligent control: it uses a negative pressure of -500 to -1000 Pa to guide spores into the device, combined with a 5-10kV electrostatic field to adsorb charged spores, and artificially simulates volatile components of Ganoderma lucidum spores such as linalool to enhance the directional aggregation efficiency of spores through chemotaxis; combined with real-time monitoring and feedback from a laser particle counter, the fan speed and collection time are dynamically adjusted; this operation can efficiently separate spores from impurities, improve product purity and collection efficiency. Compared with the shortcomings of traditional collection methods that are prone to dust and bacteria contamination, the directional collection system, through physical guidance and intelligent control, avoids the risk of chemical contamination and ensures the complete preservation of the active ingredients of the spores.

[0024] S5, Activity Retention Treatment: The collected spores were subjected to alternating short-term intense light irradiation and dark treatment using pulsed photoactivation technology. The short-term intense light irradiation used an LED light source, and the light intensity was set between 5000-10000 lux according to the characteristics of different batches of spores. During the alternation of short-term intense light irradiation and dark treatment, a pulsed magnetic field of a specific wavelength was introduced, with a magnetic field pulse frequency of 10-50 Hz and a magnetic field strength of 10-50 mT, which worked synergistically with the photoactivation process. The cell walls of the photoactivated spores were disrupted using supercritical CO2 fluid disruption technology, and then sterilized by a combination of purified water wet heat sterilization and ultraviolet synergistic treatment. The cell wall-breaking and sterilized spore powder is dried using vacuum freeze drying at a temperature of -40℃ to -30℃. The drying time is determined based on the moisture content of the spore powder, ensuring that the moisture content is below 5%. By removing minute impurities through nano-filtration, Ganoderma lucidum spore powder that enhances the body's immunity is obtained.

[0025] It should be noted that the active ingredient retention technology achieves efficient retention and safety control of Ganoderma lucidum spore powder active ingredients through a multi-step synergistic effect: it employs pulsed light activation and magnetic field synergy technology, using short-duration strong light of 5000-10000 lux to excite the photosensitivity reaction, combined with a 10-50Hz pulsed magnetic field to enhance cell membrane permeability and promote the release of active ingredients; subsequently, physical cell wall disruption is achieved through supercritical CO2 fluid permeation-decompression, avoiding the high-temperature damage effect of traditional mechanical methods; finally, vacuum freeze-drying is carried out at -40 to -30℃ to completely retain heat-sensitive components such as triterpenoids. This operation improves both the cell wall disruption rate and the retention rate of active ingredients. Compared with the shortcomings of traditional mechanical cell wall disruption, which is prone to high-temperature damage to activity and chemical methods, which have the risk of residue, this mild treatment technology, through the dual guarantee of physical means and low-temperature process, significantly improves the stability of components while ensuring safety, and simultaneously optimizes the uniformity of cell wall disruption.

[0026] Example 1 This embodiment provides a method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity, including the following steps: S1. Cultivation of new strains: Using molecular marker-assisted selection technology, strains with high β-glucan and triterpenoid content were screened from wild Ganoderma lucidum resource bank. Key gene markers related to the synthesis of immune active components were identified by gene sequencing. Using protoplast fusion technology, the selected strains with excellent traits were fused to cultivate new strains. S2. Constructing an intelligent cultivation room for cultivation: A smart cultivation room with adjustable light, temperature, humidity, and CO2 concentration was built; multiple independently controllable light sources were set up, and the switching of light and darkness was precisely controlled by a timer to simulate the photoperiod changes of Ganoderma lucidum during its natural growth season, with 12 hours of light and 12 hours of darkness alternating. Temperature and humidity sensors are used to monitor environmental parameters in the cultivation room in real time. The system automatically adjusts the temperature and humidity by using an air conditioning system and a humidifier / dehumidifier to maintain the temperature in the cultivation room at around 25°C and the relative humidity at 85%. CO2 sensors are used to monitor the CO2 concentration in the cultivation room, and the amount of CO2 entering and leaving is controlled by the ventilation system to provide a suitable CO2 environment for the growth of Ganoderma lucidum. The newly screened strains were inoculated into a smart culture room for further cultivation. S3, Aseptic log substrate composite culture: Hardwoods such as oak are selected as logs. Before drilling, the logs are sterilized by high-temperature steam and kept at 121℃ for 2-3 hours. Drill holes in the surface of the log with a diameter of 3mm and a depth of 1 / 3 of the log diameter, with a spacing of 5cm between the holes; A special culture medium was prepared by mixing sawdust and wheat bran at a mass ratio of 70:15, adding an appropriate amount of water to achieve a moisture content of 60%, and then performing composting and fermentation for 7 days, turning the compost every 2 days during the fermentation process; trace elements, including at least one of zinc, iron, manganese, and copper, were added in solution form, with the solution concentration precisely prepared according to the type and content of the element; the trace element solution was mixed with the fermented sawdust and wheat bran to obtain a special culture medium with a mass ratio of sawdust, wheat bran, and trace elements of 70:15:1. The specially prepared culture medium is filled into the drilled holes in the logs. A double-layer bagging technique is used, with an inner layer of breathable and antibacterial film and an outer layer of light-blocking bag. The new strains that have been cultivated in the intelligent cultivation room for a period of time are transferred to the logs for further cultivation. S4. Directed spore collection: A negative pressure guided collection device was used to collect spores during the spore ejection period of the new strain. The negative pressure of the negative pressure guided collection device was -500Pa. It had an electrostatic adsorption plate and a low temperature condensation system. The voltage of the electrostatic adsorption plate was set to 5kV, and the temperature of the low temperature condensation system was controlled at 0℃. The airflow was controlled by adjusting the fan speed to achieve automatic separation of spores and impurities. An odor-guiding module is set up. After analyzing the composition and proportion of the main volatile organic compounds released by Ganoderma lucidum spores during the natural spraying period using gas chromatography-mass spectrometry, artificial simulation formulation is carried out to release the specific volatile organic compounds and guide the spores into the collection device more efficiently. A laser particle counter is used to monitor the spore ejection volume in real time, and the acquisition parameters are intelligently adjusted according to the monitoring results, including adjusting the negative pressure, fan speed and acquisition time according to the changes in spore ejection volume. S5, Activity Retention Treatment: The collected spores were subjected to alternating short-term intense light irradiation and dark treatment using pulsed photoactivation technology. The short-term intense light irradiation used an LED light source, and the light intensity was set to 500 according to the characteristics of different batches of spores. During the alternation of short-term intense light irradiation and dark treatment, a pulsed magnetic field of a specific wavelength was introduced. The magnetic field pulse frequency was 10Hz and the magnetic field strength was 10mT, which worked synergistically with the photoactivation process. The cell walls of the photoactivated spores were disrupted using supercritical CO2 fluid disruption technology, and then sterilized by a combination of purified water wet heat sterilization and ultraviolet synergistic treatment. The cell wall-breaking and sterilized spore powder was dried using vacuum freeze drying at -40℃. The drying time was determined based on the moisture content of the spore powder, ensuring that the moisture content was below 5%. By removing minute impurities through nano-filtration, Ganoderma lucidum spore powder that enhances the body's immunity is obtained.

[0027] Example 2 This embodiment provides a method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity, including the following steps: S1. Cultivation of new strains: Using molecular marker-assisted selection technology, strains with high β-glucan and triterpenoid content were screened from wild Ganoderma lucidum resource bank. Key gene markers related to the synthesis of immune active components were identified by gene sequencing. Using protoplast fusion technology, the selected strains with excellent traits were fused to cultivate new strains. S2. Constructing an intelligent cultivation room for cultivation: A smart cultivation room with adjustable light, temperature, humidity, and CO2 concentration was built; multiple independently controllable light sources were set up, and the switching of light and darkness was precisely controlled by a timer to simulate the photoperiod changes of Ganoderma lucidum during its natural growth season, with 12 hours of light and 12 hours of darkness alternating. Temperature and humidity sensors are used to monitor environmental parameters in the cultivation room in real time. The system is automatically adjusted through air conditioning and humidifiers / dehumidifiers to maintain the temperature in the cultivation room at around 26°C and the relative humidity at 86%. CO2 sensors are used to monitor the CO2 concentration in the cultivation room, and the amount of CO2 entering and leaving is controlled by the ventilation system to provide a suitable CO2 environment for the growth of Ganoderma lucidum. The newly screened strains were inoculated into a smart culture room for further cultivation. S3, Aseptic log substrate composite culture: Hardwoods such as oak are selected as logs. Before drilling, the logs are sterilized by high-temperature steam and kept at 121°C for 2 hours. Drill holes in the surface of the log with a diameter of 4 mm and a depth of 1 / 2 of the log diameter. The spacing between the holes is 6 cm. A special culture medium was prepared by mixing sawdust and wheat bran at a mass ratio of 80:25, adding an appropriate amount of water to achieve a moisture content of 70%, and then performing composting and fermentation for 10 days, turning the compost every 2 days during the fermentation process. Trace elements, including at least one of zinc, iron, manganese, and copper, were added in solution form. The solution concentration was precisely prepared according to the type and content of the element. The trace element solution was mixed with the fermented sawdust and wheat bran to obtain a special culture medium with a mass ratio of sawdust, wheat bran, and trace elements of 80:25:5. The specially prepared culture medium is filled into the drilled holes in the logs. A double-layer bagging technique is used, with an inner layer of breathable and antibacterial film and an outer layer of light-blocking bag. The new strains that have been cultivated in the intelligent cultivation room for a period of time are transferred to the logs for further cultivation. S4. Directed spore collection: A negative pressure guided collection device was used to collect spores during the spore ejection period of the new strain. The negative pressure of the negative pressure guided collection device was -1000Pa. It had an electrostatic adsorption plate and a low temperature condensation system. The voltage of the electrostatic adsorption plate was set to 10kV, and the temperature of the low temperature condensation system was controlled at 5℃. The airflow was controlled by adjusting the fan speed to achieve automatic separation of spores and impurities. An odor-guiding module is set up. After analyzing the composition and proportion of the main volatile organic compounds released by Ganoderma lucidum spores during the natural spraying period using gas chromatography-mass spectrometry, artificial simulation formulation is carried out to release the specific volatile organic compounds and guide the spores into the collection device more efficiently. A laser particle counter is used to monitor the spore ejection volume in real time, and the acquisition parameters are intelligently adjusted according to the monitoring results, including adjusting the negative pressure, fan speed and acquisition time according to the changes in spore ejection volume. S5, Activity Retention Treatment: The collected spores were subjected to alternating short-term intense light irradiation and dark treatment using pulsed photoactivation technology. The short-term intense light irradiation used an LED light source, and the light intensity was set between 10,000 lux according to the characteristics of different batches of spores. During the alternation of short-term intense light irradiation and dark treatment, a pulsed magnetic field of a specific wavelength was introduced. The magnetic field pulse frequency was 50 Hz and the magnetic field strength was 50 mT, which worked synergistically with the photoactivation process. The cell walls of the photoactivated spores were disrupted using supercritical CO2 fluid disruption technology, and then sterilized by a combination of purified water wet heat sterilization and ultraviolet synergistic treatment. The cell wall-breaking and sterilized spore powder was dried using vacuum freeze drying at -30℃. The drying time was determined based on the moisture content of the spore powder, ensuring that the moisture content was below 5%. By removing minute impurities through nano-filtration, Ganoderma lucidum spore powder that enhances the body's immunity is obtained.

[0028] Example 3

[0029] This embodiment provides a method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity, including the following steps: S1. Cultivation of new strains: Using molecular marker-assisted selection technology, strains with high β-glucan and triterpenoid content were screened from wild Ganoderma lucidum resource bank. Key gene markers related to the synthesis of immune active components were identified by gene sequencing. Using protoplast fusion technology, the selected strains with excellent traits were fused to cultivate new strains. S2. Constructing an intelligent cultivation room for cultivation: A smart cultivation room with adjustable light, temperature, humidity, and CO2 concentration was built; multiple independently controllable light sources were set up, and the switching of light and darkness was precisely controlled by a timer to simulate the photoperiod changes of Ganoderma lucidum during its natural growth season, with 12 hours of light and 12 hours of darkness alternating. Temperature and humidity sensors are used to monitor environmental parameters in the cultivation room in real time. The system automatically adjusts the temperature and humidity by using an air conditioning system and a humidifier / dehumidifier to maintain the temperature in the cultivation room at around 25°C and the relative humidity at 85%. CO2 sensors are used to monitor the CO2 concentration in the cultivation room, and the amount of CO2 entering and leaving is controlled by the ventilation system to provide a suitable CO2 environment for the growth of Ganoderma lucidum. The newly screened strains were inoculated into a smart culture room for further cultivation. S3, Aseptic log substrate composite culture: Hardwoods such as oak are selected as logs. Before drilling, the logs are sterilized by high-temperature steam and kept at 121°C for 2 hours. Drill holes in the surface of the log with a diameter of 4 mm and a depth of 1 / 2 of the log diameter. The spacing between the holes is 6 cm. A special culture medium was prepared by mixing sawdust and wheat bran at a mass ratio of 80:25, adding an appropriate amount of water to achieve a moisture content of 70%, and then performing composting and fermentation for 10 days, turning the compost every 2 days during the fermentation process. Trace elements, including at least one of zinc, iron, manganese, and copper, were added in solution form. The solution concentration was precisely prepared according to the type and content of the element. The trace element solution was mixed with the fermented sawdust and wheat bran to obtain a special culture medium with a mass ratio of sawdust, wheat bran, and trace elements of 80:25:5. The specially prepared culture medium is filled into the drilled holes in the logs. A double-layer bagging technique is used, with an inner layer of breathable and antibacterial film and an outer layer of light-blocking bag. The new strains that have been cultivated in the intelligent cultivation room for a period of time are transferred to the logs for further cultivation. S4. Directed spore collection: A negative pressure guided collection device was used to collect spores during the spore ejection period of the new strain. The negative pressure of the negative pressure guided collection device was -1000Pa. It had an electrostatic adsorption plate and a low temperature condensation system. The voltage of the electrostatic adsorption plate was set to 10kV, and the temperature of the low temperature condensation system was controlled at 5℃. The airflow was controlled by adjusting the fan speed to achieve automatic separation of spores and impurities. An odor-guiding module is set up. After analyzing the composition and proportion of the main volatile organic compounds released by Ganoderma lucidum spores during the natural spraying period using gas chromatography-mass spectrometry, artificial simulation formulation is carried out to release the specific volatile organic compounds and guide the spores into the collection device more efficiently. A laser particle counter is used to monitor the spore ejection volume in real time, and the acquisition parameters are intelligently adjusted according to the monitoring results, including adjusting the negative pressure, fan speed and acquisition time according to the changes in spore ejection volume. S5, Activity Retention Treatment: The collected spores were subjected to alternating short-term intense light irradiation and dark treatment using pulsed photoactivation technology. The short-term intense light irradiation used an LED light source, and the light intensity was set between 10,000 lux according to the characteristics of different batches of spores. During the alternation of short-term intense light irradiation and dark treatment, a pulsed magnetic field of a specific wavelength was introduced. The magnetic field pulse frequency was 50 Hz and the magnetic field strength was 50 mT, which worked synergistically with the photoactivation process. The cell walls of the photoactivated spores were disrupted using supercritical CO2 fluid disruption technology, and then sterilized by a combination of purified water wet heat sterilization and ultraviolet synergistic treatment. The cell wall-breaking and sterilized spore powder was dried using vacuum freeze drying at -30℃. The drying time was determined based on the moisture content of the spore powder, ensuring that the moisture content was below 5%. By removing minute impurities through nano-filtration, Ganoderma lucidum spore powder that enhances the body's immunity is obtained.

[0030] Example 4

[0031] This embodiment provides a method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity, including the following steps: S1. Cultivation of new strains: Using molecular marker-assisted selection technology, strains with high β-glucan and triterpenoid content were screened from wild Ganoderma lucidum resource bank. Key gene markers related to the synthesis of immune active components were identified by gene sequencing. Using protoplast fusion technology, the selected strains with excellent traits were fused to cultivate new strains. S2. Constructing an intelligent cultivation room for cultivation: A smart cultivation room with adjustable light, temperature, humidity, and CO2 concentration was built; multiple independently controllable light sources were set up, and the switching of light and darkness was precisely controlled by a timer to simulate the photoperiod changes of Ganoderma lucidum during its natural growth season, with 12 hours of light and 12 hours of darkness alternating. Temperature and humidity sensors are used to monitor environmental parameters in the cultivation room in real time. The system is automatically adjusted through air conditioning and humidifiers / dehumidifiers to maintain the temperature in the cultivation room at around 26°C and the relative humidity at 86%. CO2 sensors are used to monitor the CO2 concentration in the cultivation room, and the amount of CO2 entering and leaving is controlled by the ventilation system to provide a suitable CO2 environment for the growth of Ganoderma lucidum. The newly screened strains were inoculated into a smart culture room for further cultivation. S3, Aseptic log substrate composite culture: Hardwoods such as oak are selected as logs. Before drilling, the logs are sterilized by high-temperature steam at 121°C for 2.5 hours. Drill holes in the surface of the log with a diameter of 4mm and a depth of 1 / 2 of the log diameter. The spacing between the holes is 5-8cm. A special culture medium was prepared by mixing sawdust and wheat bran at a mass ratio of 75:20, adding an appropriate amount of water to achieve a moisture content of 65%, and then performing composting and fermentation for 8 days, turning the compost every 2.5 days during the fermentation process; trace elements, including at least one of zinc, iron, manganese, and copper, were added in solution form, with the solution concentration precisely prepared according to the type and content of the element; the trace element solution was mixed with the fermented sawdust and wheat bran to obtain a special culture medium with a mass ratio of sawdust, wheat bran, and trace elements of 79:24:4. The specially prepared culture medium is filled into the drilled holes in the logs. A double-layer bagging technique is used, with an inner layer of breathable and antibacterial film and an outer layer of light-blocking bag. The new strains that have been cultivated in the intelligent cultivation room for a period of time are transferred to the logs for further cultivation. S4. Directed spore collection: A negative pressure guided collection device was used to collect spores during the spore ejection period of the new strain. The negative pressure of the negative pressure guided collection device was -550Pa. It had an electrostatic adsorption plate and a low temperature condensation system. The voltage of the electrostatic adsorption plate was set to 7kV, and the temperature of the low temperature condensation system was controlled at 4℃. The airflow was controlled by adjusting the fan speed to achieve automatic separation of spores and impurities. An odor-guiding module is set up. After analyzing the composition and proportion of the main volatile organic compounds released by Ganoderma lucidum spores during the natural spraying period using gas chromatography-mass spectrometry, artificial simulation formulation is carried out to release the specific volatile organic compounds and guide the spores into the collection device more efficiently. A laser particle counter is used to monitor the spore ejection volume in real time, and the acquisition parameters are intelligently adjusted according to the monitoring results, including adjusting the negative pressure, fan speed and acquisition time according to the changes in spore ejection volume. S5, Activity Retention Treatment: The collected spores were subjected to alternating short-term intense light irradiation and dark treatment using pulsed photoactivation technology. The short-term intense light irradiation used an LED light source, and the light intensity was set to 6000 lux according to the characteristics of different batches of spores. During the alternation of short-term intense light irradiation and dark treatment, a pulsed magnetic field of a specific wavelength was introduced, with a magnetic field pulse frequency of 40 Hz and a magnetic field strength of 30 mT, which worked synergistically with the photoactivation process. The cell walls of the photoactivated spores were disrupted using supercritical CO2 fluid disruption technology, and then sterilized by a combination of purified water wet heat sterilization and ultraviolet synergistic treatment. The cell wall-breaking and sterilized spore powder was dried using vacuum freeze drying at -35℃. The drying time was determined based on the moisture content of the spore powder, ensuring that the moisture content was below 5%. By removing minute impurities through nano-filtration, Ganoderma lucidum spore powder that enhances the body's immunity is obtained.

[0032] Example 5 This embodiment provides a method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity, including the following steps: S1. Cultivation of new strains: Using molecular marker-assisted selection technology, strains with high β-glucan and triterpenoid content were screened from wild Ganoderma lucidum resource bank. Key gene markers related to the synthesis of immune active components were identified by gene sequencing. Using protoplast fusion technology, the selected strains with excellent traits were fused to cultivate new strains. S2. Constructing an intelligent cultivation room for cultivation: A smart cultivation room with adjustable light, temperature, humidity, and CO2 concentration was built; multiple independently controllable light sources were set up, and the switching of light and darkness was precisely controlled by a timer to simulate the photoperiod changes of Ganoderma lucidum during its natural growth season, with 12 hours of light and 12 hours of darkness alternating. Temperature and humidity sensors are used to monitor environmental parameters in the cultivation room in real time. The system is automatically adjusted through air conditioning and humidifiers / dehumidifiers to maintain the temperature in the cultivation room at around 26°C and the relative humidity at 86%. CO2 sensors are used to monitor the CO2 concentration in the cultivation room, and the amount of CO2 entering and leaving is controlled by the ventilation system to provide a suitable CO2 environment for the growth of Ganoderma lucidum. The newly screened strains were inoculated into a smart culture room for further cultivation. S3, Aseptic log substrate composite culture: Hardwoods such as oak are selected as logs. Before drilling, the logs are sterilized by high-temperature steam and kept at 121°C for 3 hours. Drill holes in the surface of the log with a diameter of 4 mm and a depth of 1 / 2 of the log diameter. The spacing between the holes is 7 cm. A special culture medium was prepared by mixing sawdust and wheat bran at a mass ratio of 76:23, adding an appropriate amount of water to achieve a moisture content of 68%, and then carrying out composting and fermentation for 8 days, turning the compost every 2.5 days during the fermentation process; the trace element zinc was added in solution form, and the trace element solution was mixed with the fermented sawdust and wheat bran to obtain a special culture medium with a mass ratio of sawdust, wheat bran and trace element of 75:15:2. The specially prepared culture medium is filled into the drilled holes in the logs. A double-layer bagging technique is used, with an inner layer of breathable and antibacterial film and an outer layer of light-blocking bag. The new strains that have been cultivated in the intelligent cultivation room for a period of time are transferred to the logs for further cultivation. S4. Directed spore collection: A negative pressure guided collection device was used to collect spores during the spore ejection period of the new strain. The negative pressure of the negative pressure guided collection device was -700Pa. It had an internal electrostatic adsorption plate and a low temperature condensation system. The voltage of the electrostatic adsorption plate was set to 9kV, and the temperature of the low temperature condensation system was controlled at 1℃. The airflow was controlled by adjusting the fan speed to achieve automatic separation of spores and impurities. An odor-guiding module is set up. After analyzing the composition and proportion of the main volatile organic compounds released by Ganoderma lucidum spores during the natural spraying period using gas chromatography-mass spectrometry, artificial simulation formulation is carried out to release the specific volatile organic compounds and guide the spores into the collection device more efficiently. A laser particle counter is used to monitor the spore ejection volume in real time, and the acquisition parameters are intelligently adjusted according to the monitoring results, including adjusting the negative pressure, fan speed and acquisition time according to the changes in spore ejection volume. S5, Activity Retention Treatment: The collected spores were subjected to alternating short-term intense light irradiation and dark treatment using pulsed photoactivation technology. The short-term intense light irradiation used an LED light source, and the light intensity was set between 8000 lux according to the characteristics of different batches of spores. During the alternation of short-term intense light irradiation and dark treatment, a pulsed magnetic field of a specific wavelength was introduced. The magnetic field pulse frequency was 30 Hz and the magnetic field strength was 20 mT, which worked synergistically with the photoactivation process. The cell walls of the photoactivated spores were disrupted using supercritical CO2 fluid disruption technology, and then sterilized by a combination of purified water wet heat sterilization and ultraviolet synergistic treatment. The cell wall-breaking and sterilized spore powder was dried using vacuum freeze drying at -36℃. The drying time was determined based on the moisture content of the spore powder, ensuring that the moisture content was below 5%. By removing minute impurities through nano-filtration, Ganoderma lucidum spore powder that enhances the body's immunity is obtained.

[0033] Comparative Example 1 Except for replacing the cultivation of new strains with the selection of Ganoderma lucidum from existing Ganoderma lucidum cultivation bases, the other steps are exactly the same as in Example 1.

[0034] Comparative Example 2 Replacing the intelligent cultivation room with an existing ordinary greenhouse as the cultivation room means that it is impossible to precisely control the light, temperature, humidity and CO2 concentration. The light duration depends on the natural light conditions, and the temperature, humidity and CO2 concentration fluctuate with changes in the external environment. The rest of the operation is exactly the same as in Example 2.

[0035] Comparative Example 3 The aseptic log substrate composite culture was replaced with conventional ordinary wood culture. That is, ordinary wood was selected as the log, no high-temperature steam sterilization was performed, the drilling parameters were set arbitrarily, ordinary culture medium was used, no fermentation treatment was performed, double-layer bagging technology was not used, and the strain was directly inoculated onto the log for culture. The rest of the operation was exactly the same as in Example 3.

[0036] Comparative Example 4 The directional spore collection was replaced with the existing ordinary negative pressure collection, without electrostatic adsorption plates, low temperature condensation systems and odor guiding modules. The collection parameters were adjusted manually based on experience. The rest of the operation was exactly the same as in Example 4.

[0037] Comparative Example 5 The supercritical CO2 fluid cell disruption operation was replaced with conventional mechanical grinding and chemical enzymatic hydrolysis, and finally conventional drying and sterilization were performed. The remaining operations were exactly the same as in Example 5.

[0038] Based on the above embodiments and comparative examples, we obtain: Table 1: project Example 1: New strain Comparative Example 1: Existing Plants β-glucan content (mg / g) 12.5 8.2 Triterpenoid content (mg / g) 9.8 6.5 Expression levels (relative values) of key genes related to the synthesis of immune-active components 3.2 1.5 Based on the data in the table above, it can be seen that the new strain cultivated in Example 1 using molecular marker-assisted selection and protoplast fusion technology has significantly higher β-glucan and triterpenoid content than the existing plants selected in Comparative Example 1, and also has higher expression levels of key genes related to the synthesis of immune active components. This indicates that the new strain cultivation method can greatly improve screening efficiency and accuracy, and quickly obtain strains that produce high levels of immune active components, thus ensuring the quality of Ganoderma lucidum spore powder and its immune-enhancing effect from the source. Table 2: project Example 2: Intelligent Cultivation Room Comparative Example 2: Existing Cultivation Room Reishi mushroom growth cycle (days) 90 110 Reishi mushroom cap diameter (cm) 15.2 12.5 β-glucan content (mg / g) 13.0 9.5 Triterpenoid content (mg / g) 10.2 7.0 Based on the data in the table above, it can be seen that the Ganoderma lucidum cultivated in the intelligent cultivation room in Example 2 has a shorter growth cycle, a larger cap diameter, and higher contents of β-glucan and trimembrane than the Ganoderma lucidum cultivated in the existing cultivation room in Comparative Example 2. This indicates that the intelligent cultivation room provides the most suitable environmental conditions for the growth of Ganoderma lucidum by accurately simulating the photoperiod changes of the natural growth season of Ganoderma lucidum, which can promote the growth of Ganoderma lucidum and the synthesis and accumulation of immune active components. Table 3: project Example 3: Aseptic Wood Matrix Composite Culture Comparative Example 3: Conventional Cultivation Log contamination rate (%) 5 25 Ganoderma lucidum spore powder yield (g / log section) 120 80 Impurity content (%) in spore powder 2 8 Based on the data in the table above, it can be seen that in Example 3, the use of sterile logs and double-layer bagging technology resulted in a significantly lower log contamination rate than the conventional cultivation method in Comparative Example 3. The yield of Ganoderma lucidum spore powder was higher, and the impurity content in the spore powder was lower. This indicates that the sterile logs and double-layer bagging technology can effectively reduce contamination, provide a cleaner environment for Ganoderma lucidum growth, and thus improve the yield and purity of spore powder. Table 4: project Example 4 Comparative Example 4 Spore collection efficiency (%) 90 65 Spore purity (%) 98 85 Data collection time (hours) 8 12 Based on the data in the table above, it can be seen that the negative pressure guided collection device with an odor guiding module in Example 4 has a significantly higher spore collection efficiency than the existing collection method in Comparative Example 4, with higher spore purity and shorter collection time. This indicates that the odor guiding module can guide spores into the collection device more efficiently, improving collection efficiency and spore purity, and reducing impurity contamination. Table 5: project Example 5 Comparative Example 5 Cell wall breakage rate (%) 95 70 Active substance extraction rate (%) 90 60 Hygiene and safety indicators for spore powder (total bacterial count, CFU / g) ＜10 ＞100 Spore powder stability (change rate of active substance content after 6 months of storage) 5% 20% Based on the data in the table above, it can be seen that Example 5, which uses supercritical CO2 cell disruption technology, has a significantly higher cell disruption rate and active substance extraction rate than the traditional method in Comparative Example 5. The spore powder has better hygiene and safety indicators, and the change rate of active substance content is lower after 6 months of storage, indicating better stability. This shows that supercritical CO2 cell disruption technology can release intracellular active substances more thoroughly, improve extraction efficiency, and has a good cell disruption effect while ensuring the hygiene, safety, activity, and stability of the spore powder.

[0039] The terms "front," "back," "left," "right," "top," and "bottom" all refer to the figures in the accompanying drawings. Figure 1 Based on the perspective of the observer, the side of the device facing the observer is defined as the front, the left side of the observer is defined as the left, and so on.

[0040] In the description of this invention, it should be understood that the terms "center", "longitudinal", "lateral", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limiting the scope of protection of this invention.

[0041] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the present invention as claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cultivation method of ganoderma spore powder for enhancing immunity of a body, characterized by, The method comprises the following steps: S1, using molecular marker assisted selection technology, screening strains with high content of β-glucan and triterpenoids from wild ganoderma resource library, and determining key gene markers related to synthesis of immune active ingredients through gene sequencing, using protoplast fusion technology to fuse strains with excellent traits screened out to cultivate new strains; S2, building an intelligent cultivation room that can regulate light, temperature and humidity, CO2 concentration to simulate the light cycle changes of ganoderma natural growth season, and inoculating the new strains screened and cultivated into the intelligent cultivation room for cultivation; S3, selecting hardwood such as oak as a segment wood, drilling holes on the surface of the segment wood, filling special culture medium, and using double-layer bagging technology to transfer the new strains cultivated in the intelligent cultivation room for a period of time to the segment wood for further cultivation; The inner layer of the double-layer bagging technology is a breathable bacteria-proof film, and the outer layer is a light shielding bag; S4, using negative pressure guided collection device to collect spores in the spore spraying period of the new strain, and automatically separating impurities; S5, using pulse light activation technology to alternately irradiate the collected spores with short time strong light and dark treatment, and using supercritical CO2 fluid breaking wall technology combined with sterilization technology; Through nanoscale filtration to remove tiny impurities, ganoderma spore powder for enhancing immune function of the body is obtained.

2. The method for cultivating ganoderma lucidum spore powder for enhancing immunity according to claim 1, characterized in that: The S1 step specifically comprises: Extracting DNA of each strain in the wild ganoderma resource library, using specific primers of genes related to synthesis of β-glucan and triterpenoids for PCR amplification, and selecting positive strains according to the amplification results; When determining the key gene markers by gene sequencing, high-throughput sequencing technology is used to sequence the whole genome of the selected positive strains, and the gene fragments closely related to the synthesis of immune active ingredients are found as key gene markers through bioinformatics analysis; In the protoplast fusion technology, the protoplasts of the selected excellent strains are prepared first, and then polyethylene glycol is used as a fusion agent to promote the fusion of protoplasts, and new strains with high yield of immune active ingredients are obtained through screening and identification.

3. The method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity according to claim 2, characterized in that: In the S2 step, the light regulation of the intelligent cultivation room is realized by setting multiple independently controllable light sources; The temperature and humidity regulation adopts a temperature and humidity sensor to monitor the environmental parameters in the cultivation room in real time, and automatically adjusts the temperature and humidity through an air conditioning system and a humidifier / dehumidifier, so as to maintain the temperature in the cultivation room at 25-28℃ and the relative humidity at 85%-90%; The CO2 concentration regulation is monitored by a CO2 sensor, and the amount of CO2 in and out is controlled by a ventilation system; When simulating the light cycle changes, the time switching of light and darkness is accurately controlled by a timer, wherein the light cycle changes are embodied by setting 12 hours of light and 12 hours of darkness alternately.

4. The method for cultivating ganoderma lucidum spore powder for enhancing immunity according to claim 3, characterized in that: In the S3 step: The segment wood is subjected to high-temperature steam sterilization treatment before drilling, and the high-temperature steam sterilization treatment is performed at 121℃ for 2-3 hours; The hole diameter of the segment wood is 3-5mm, the hole depth is 1 / 3-1 / 2 of the diameter of the segment wood, and the drilling interval is 5-8cm; The special culture medium is composed of sawdust, wheat bran and trace elements, and the mass ratio of the sawdust, the wheat bran and the trace elements is 70-80:15-25:1-5, and the trace elements include at least one of zinc, iron, manganese and copper.

5. The method for cultivating ganoderma lucidum spore powder for enhancing immunity according to claim 1, characterized in that: The special culture medium is pretreated before filling: After the sawdust and the wheat bran are mixed, an appropriate amount of water is added to make the water content reach 60%-70%, and then the fermentation treatment is performed by stacking, and the fermentation time is 7-10 days, and the stacking is turned over every 2-3 days during the fermentation process; The trace elements are added in the form of a solution, and the solution concentration is accurately prepared according to the element type and content.

6. The method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity according to claim 4, characterized in that: In the S4 step: The negative pressure range of the negative pressure guiding type collection device is-500Pa to-1000Pa, and the negative pressure guiding type collection device is internally provided with an electrostatic adsorption plate and a low-temperature condensation system, and the automatic separation of spores and impurities is realized through airflow control, wherein the voltage of the electrostatic adsorption plate is set to 5-10kV, the temperature of the low-temperature condensation system is controlled at 0-5℃, and the airflow control is realized by adjusting the fan speed.

7. The method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity according to claim 1, characterized in that: The S4 also includes real-time monitoring of the spore injection amount by using a laser particle counter, and intelligently adjusting the collection parameters according to the monitoring results; The intelligent adjustment of the collection parameters includes adjusting the negative pressure size, the fan speed and the collection time according to the change of the spore injection amount.

8. The method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity according to claim 1, characterized in that: In the negative pressure guiding type collection device, an odor guiding module is also arranged, which releases specific volatile organic compounds emitted by the ganoderma spores; The specific volatile organic compounds are the main volatile components released by the ganoderma spores during the natural injection period, and the composition and proportion thereof are determined by gas chromatography-mass spectrometry technology analysis, and then artificial simulation preparation is performed.

9. The method for cultivating Ganoderma lucidum spore powder to enhance the body's immunity according to claim 1, characterized in that: In the S5 step: The short-time strong light irradiation of the pulse type light activation technology uses an LED light source, and the light intensity is set to 5000-10000lux according to the characteristics of different batches of spores; The spore powder treated by the supercritical CO2 fluid wall breaking technology needs to be dried, and the drying method adopts vacuum freeze drying, the drying temperature is-40℃ to-30℃, and the drying time is determined according to the water content of the spore powder, and the water content of the spore powder is less than 5%; The sterilization technology uses purified water wet heat sterilization combined with ultraviolet synergistic treatment.

10. The method for cultivating ganoderma lucidum spore powder for enhancing immunity of the body according to claim 9, characterized in that: In the pulse type light activation technology, a specific wavelength pulse magnetic field is introduced in the alternating process of short-time strong light irradiation and dark treatment, which cooperates with the light activation process; The magnetic field pulse frequency is 10-50Hz, and the magnetic field strength is 10-50mT.

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