Beauty nursing dressing with vinegar residues, eutectic solvent and CO2 as core raw materials
By using vinegar residue, eutectic solvent and CO2 as core raw materials, a composite cosmetic dressing has been developed, which solves the problems of limited functionality and environmental pollution of existing dressings and achieves multifunctional wound healing and scar repair effects.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-02
- Publication Date
- 2026-03-10
AI Technical Summary
Existing cosmetic dressings have limited functionality in preventing scar formation and promoting wound healing, and waste products from the brewing industry, such as vinegar residue, are not effectively utilized, leading to environmental pollution problems.
Using vinegar residue, eutectic solvent, and CO2 as core raw materials, a composite cosmetic care dressing is prepared. By utilizing the natural active ingredients in vinegar residue, the dissolving power of the eutectic solvent, and the antibacterial effect of CO2, combined with polyvinyl alcohol and chitosan quaternary ammonium salt, a multifunctional care dressing is formed.
It achieves multifunctional nursing dressings, including antioxidant, anti-inflammatory, antibacterial, wound healing and scar repair properties, improves the film-forming and adhesive properties of dressings, and reduces environmental pollution.
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Abstract
Description
TECHNICAL FIELD
[0001] The present application belongs to the technical field of cosmetic care dressings, and particularly relates to a composite component cosmetic care dressing derived from brewing industry waste and having multiple functions and a preparation method thereof. BACKGROUND
[0002] In the recovery stage of a wound, if the wound surface cannot be properly maintained in a clean and dry environment, or the importance of timely dressing change is ignored, wound infection or inflammatory response may be caused. Once infection occurs, excessive collagen and other repair substances will be produced in the wound, which is beneficial to healing on the one hand, but may also interfere with the normal repair trajectory of the skin, ultimately leading to the formation of scars. In addition, when the skin is subjected to strong stimuli such as external impact or surgical incision, the internal elastic fibers may be broken, which is another major cause of scar formation. Since the resulting scars affect the appearance, how to effectively prevent the formation of scars and explore related cosmetic care methods has always been an important concern in post-care.
[0003] Hydrogel, as a commonly used material for cosmetic care dressings, has good mechanical properties and biocompatibility. Its structure is similar to that of living tissues, and it has weak adhesion to proteins and cells. When it comes into contact with blood, body fluids and human tissues, it shows obvious friendliness; it does not affect the metabolic process of living organisms, and the metabolic products can be discharged through the hydrogel, and it is similar in nature to part of the extracellular matrix. After absorbing water, it can reduce the friction and mechanical action on the surrounding tissues, and improve the biological properties of the material. In addition, hydrogel can swell or shrink with changes in temperature, pH, electric field or chemical substances. Hydrogel dressings have a series of advanced functions, including adhesion, hemostasis, antibacterial, anti-inflammatory and antioxidant, nutrient delivery, self-healing, electrical conductivity, and can monitor the wound. Currently, the functions of hydrogel have changed from the original single physical coverage of dressings to the current multi-functionalization to meet various complex cosmetic care needs.
[0004] Vinegar residue, as a by-product of vinegar production, is usually discarded, incinerated or landfilled as a worthless waste. However, with the rapid development of modern biotechnology and material science, people have begun to re-examine the potential value of these "waste". Among them, the rich natural active ingredients contained in vinegar residue are gradually attracting great interest in the medical and beauty fields due to their unique biological activity and pharmacological effects. The main components of vinegar residue are cellulose, lignin, hemicellulose, and residual starch, protein, etc. (Ma X Z. Jiangsu Condiment and Foodstuff, 2005, 22(2): 32-34). In addition to the characteristic raw materials in vinegar koji (many varieties also contain Chinese herbal medicines), a series of substances with different biological activities can be produced after fermentation, such as organic acids, enzymes, polyphenols, amino acids, etc. The presence of these substances makes it have the potential to scavenge free radicals around the wound, reduce the damage of oxidative stress to wound tissue; at the same time, it can promote the proliferation and differentiation of wound cells, accelerate the process of wound repair, healing and convergence. Therefore, the application of vinegar residue raw materials in beauty care dressings can play an active role, not only beneficial to tap its potential added value, but also reduce the environmental burden and extend the industrial chain.
[0005] Deep eutectic solvent (DES) is a new type of multi-purpose green solvent composed of hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA) in a certain molar ratio, which exists in liquid form at room temperature. Its application in the field of health is still in its infancy. Due to its unique physicochemical properties, it has been found to be able to dissolve a variety of poorly soluble macromolecules represented by cellulose and a variety of active small molecules. In addition, it has good biocompatibility and the characteristics of promoting delivery and penetration, which makes it have great potential in the application direction of external use of transdermal absorption. It can not only be used to dissolve various beneficial components in vinegar residue, but also produce positive interaction with hydrogel matrix, significantly improve the film-forming property of dressing and its adhesion to the wound surface, so that the dressing can be more firmly attached to the wound. These new green solvents and their dissolved components can not only optimize the microenvironment of the wound, but also have antibacterial effect, which can inhibit the growth of microorganisms and significantly reduce the risk of infection. The above effects collectively promote the rapid proliferation and orderly migration of wound cells, accelerate the repair process of scar and the recovery of muscle surface. Overall, it can achieve the effect of "one stone, many birds" in hydrogel beauty care dressing, which not only makes full use of its multifunctionality, but also greatly simplifies the composition of the system.
[0006] CO2 has a variety of applications in the medical field, but mainly focuses on medical equipment and treatment methods, such as CO2 laser treatment, etc. There is no report on the direct addition of CO2 in wound care dressings. CO2 can form carbonic acid in water, thereby adjusting the pH of the dressing (Fan Q. Food Safety Guide, 2017, 11 (31): 52-54). The appropriate pH may have a certain promoting effect on wound healing and scar recovery. Studies have shown that low concentrations of CO2 have inhibitory effects on some bacteria, which may help reduce the risk of wound infection (Mikael P., et al., Medical Hypotheses, 2008, 71 (1): 8-13). If this effect can be realized in wound dressings, it may help improve blood circulation at the wound site, thereby promoting scar recovery. Enfors et al. (Enfors S.O., Journal of Applied Bacteriology, 1979, 47: 197-208.) also studied the effect of CO2 on the germination of Clostridium spores, Clostridium perfringens and Bacillus cereus spores in complex media. The germination experiment was carried out at pH 5.2-6.7 and atmospheric pressure (1 atm). The results showed that CO2 under these conditions can inhibit the germination of Bacillus cereus spores. The above studies have proved that CO2 has a significant bacteriostatic effect, making it have great application potential in nursing dressings. SUMMARY
[0007] The application discloses a cosmetic nursing dressing taking vinegar residue, a eutectic solvent and CO2 as core raw materials. The vinegar residue is from a waste of a brewing industry, the CO2 is used as an auxiliary functional material, and the eutectic solvent comprises choline chloride-oxalic acid, choline chloride-lactic acid, citric acid-xylose alcohol-water, tetrabutylammonium chloride-oxalic acid, ethylene glycol-oxalic acid and 1, 4-butanediol-oxalic acid, and is used as a solvent of the vinegar residue component, a CO2 carrier and a film-forming aid. The vinegar residue-eutectic solvent solution with solidified CO2 under high pressure is prepared into a mixed system together with polyvinyl alcohol and a chitosan quaternary ammonium salt aqueous solution, and finally, a composite cosmetic nursing dressing is obtained by using a flow casting method. The preparation process is simple, friendly and low in cost, and has strong film-forming property; the obtained dressing product is good in gloss, flexibility, uniformity and adhesion, and also has antioxidant, anti-karatin, tyrosinase inhibition and bacteriostatic activities, and realizes multifunctionalization of the nursing dressing and high-value development of the vinegar residue waste resources.
[0008] Technical scheme: In order to realize the above object, a cosmetic nursing dressing taking vinegar residue, a eutectic solvent and CO2 as core raw materials is provided.
[0009] The cosmetic care dressing with vinegar residue, eutectic solvent and CO2 as core raw materials comprises vinegar residue component, eutectic solvent, CO2 and other auxiliary materials.
[0010] The cosmetic care dressing with vinegar residue, eutectic solvent and CO2 as core raw materials is characterized in that the vinegar residue is obtained from the waste of brewing industry, and is degreased by petroleum ether and then dissolved by eutectic solvent to obtain vinegar residue-eutectic solvent solution.
[0011] The cosmetic care dressing with vinegar residue, eutectic solvent and CO2 as core raw materials is characterized in that the eutectic solvent includes but is not limited to choline chloride-oxalic acid, choline chloride-lactic acid, citric acid-xylose alcohol-water, tetrabutylammonium chloride-oxalic acid, ethylene glycol-oxalic acid and 1,4-butanediol-oxalic acid, which are used as vinegar residue component solvent, CO2 carrier and film forming aid.
[0012] The cosmetic care dressing with vinegar residue, eutectic solvent and CO2 as core raw materials is characterized in that CO2 is used as auxiliary function material and is pressed into the vinegar residue-eutectic solvent solution under pressure.
[0013] The cosmetic care dressing with vinegar residue, eutectic solvent and CO2 as core raw materials is characterized in that the other auxiliary materials are polyvinyl alcohol and chitosan quaternary ammonium salt.
[0014] The cosmetic care dressing with vinegar residue, eutectic solvent and CO2 as core raw materials is characterized in that the specific steps for preparing the dressing are as follows: (1) The vinegar residue is degreased by petroleum ether with boiling range of 30-60 DEG C according to solid-liquid ratio of 1:1 (g / mL), heated at 30-60 DEG C for 2-3 h, filtered and dried to obtain the degreased vinegar residue; (2) The degreased vinegar residue is mixed with the eutectic solvent according to solid-liquid ratio of 1:5-1:40 (g / mL), heated at 40-90 DEG C for 1-3 h, filtered and collected to obtain vinegar residue-eutectic solvent solution; (3) CO2 is pressed into the vinegar residue-eutectic solvent solution under pressure of 200-600 kPa for 1-6 h; (4) Chitosan quaternary ammonium salt and polyvinyl alcohol are mixed according to solid-solid ratio of 0.1:1-1:1 (g / g), deionized water is gradually added and heated and stirred at 40 DEG C until they are just dissolved to obtain saturated solution of the two; the CO2-loaded vinegar residue-eutectic solvent solution is added into the saturated solution according to liquid-liquid ratio of 0.6:1-1.6:1 (mL / mL) and mixed uniformly. (5) All the film paste is poured on a smooth and clean glass plane, and the thickness is controlled to be 0.5-1 mm after natural flow. (6) The film is naturally air-dried for 18-48 hours, and the film is removed after complete solidification; (7) The prepared dressing product is cut into a certain specification, sterilized and disinfected under ultraviolet lamp irradiation for 60 min, and then stored after being packed and sealed. BRIEF DESCRIPTION OF DRAWINGS
[0015] In order to more completely explain the technical solutions of the embodiments of the present application, the required drawings in the embodiments will be briefly described below. The described drawings are only a part of the embodiments of the present application, and other drawings of the embodiments can be obtained by those skilled in the art according to the drawings. Among them:
[0016] Figure 1 The basic component analysis results of the vinegar residue in the present application.
[0017] Figure 2 The CO2 molar ratio (NCO2) of the vinegar residue-hydropolymers dissolved solution in the present application is solidified at 300 kPa. CO2 ).
[0018] Figure 3 The appearance comparison of the prepared product of the cosmetic care dressing and related components in the present application.
[0019] Figure 4 The slack, stretch, and twist states of the cosmetic care dressing in the present application.
[0020] Figure 5 The adhesion of the cosmetic care dressing in the present application on various surfaces, specifically including glass, paper, polytetrafluoroethylene (PTFE), plastics, skin, and pig skin.
[0021] Figure 6 The evaluation results of the keratin activity (Ca 2+ ) of the cosmetic care dressing in the present application (the liquid-liquid ratio of PVA+CSQA saturated solution and vinegar residue-hydropolymers dissolved solution is 1:0.4-0.8).
[0022] Figure 7 The evaluation results of the antioxidant activity of the cosmetic care dressing in the present application.
[0023] Figure 8 The evaluation results of the tyrosinase inhibitory activity of the cosmetic care dressing in the present application (the liquid-liquid ratio of PVA+CSQA saturated solution and vinegar residue-hydropolymers dissolved solution is 1:0.4-0.8). DETAILED DESCRIPTION
[0024] The following is a detailed description of the application. For the purpose of promoting an understanding of the application, the application will be described in relation to exemplary embodiments thereof and variations thereof, and variants thereof. However, it is to be understood that no limitation of the scope of the application is intended by the description of the embodiments.
[0025] The specification and claims herein are not to be construed as limiting the application based on differences in word usage, but rather on the functional differences between the components. The use of "including," "including but not limited to," or "having" herein is not limiting, and is understood to encompass the terms "comprising" and / or "consisting of." The embodiments described later in the specification are intended to be illustrative of the application and are not exhaustive, but rather are presented to assist in the full understanding of the application.
[0026] Example 1
[0027] The vinegar residue was degreased using petroleum ether with a boiling range of 30-60°C at a solid-liquid ratio of 1:1 (g / mL) for 2 h at 60°C, filtered and dried to obtain the degreased vinegar residue; the moisture content of the vinegar residue sample was determined according to the reduced pressure drying method specified in the national standard GB / T6435-2014; the ash content of the vinegar residue was characterized according to the national standard GB / T 6438-2007; the crude protein content of the vinegar residue was characterized according to the Kjeldahl method in the national standard GB / T 6432-2018; the crude fat content of the vinegar residue sample was determined in detail according to the national standard GB / T6433-2006; the neutral detergent fiber (NDF) content of the vinegar residue sample was quantitatively analyzed according to the requirements of the national standard GB / T20806-2006; the acid detergent fiber (ADF) content of the vinegar residue sample was determined following the guidance of the national agricultural industry standard NY / T 1459-2007; and the acid detergent lignin (ADL) content of the vinegar residue sample was determined following the national standard GB / T 20805-2006. All the results are shown in Table 1. Figure 1
[0028] Example 2
[0029] The eutectic solvent choline chloride-oxalic acid (molar ratio 1:1) was mixed with defatted vinegar residue at a solid-liquid ratio of 1:10 (g / mL) until homogeneous. The mixture was heated at 60℃ for 3 hours and filtered to obtain a choline chloride-oxalic acid solution of the vinegar residue. CO2 was injected into the vinegar residue-eutectic solvent solution at a pressure of 300 kPa for 2 hours. Chitosan quaternary ammonium salt (CSQA) and polyvinyl alcohol (PVA) were mixed at a solid-solid ratio of 0.6:1, and deionized water was gradually added and heated at 40℃ until just dissolved to obtain a saturated solution of both. The vinegar residue solution with CO2 immobilized was then added to the saturated solution at a liquid-liquid ratio of 0.7:1 (mL / mL) and mixed until homogeneous. The membrane slurry was poured onto a smooth and clean glass surface and allowed to flow naturally with a thickness controlled at 0.8 mm. After standing and air drying for 48 hours, the product dressing membrane was obtained. After sterilization by irradiation with ultraviolet light for 60 minutes, it was dispensed and sealed for storage.
[0030] Figure 2 This shows the CO2 molar ratio (N) of the vinegar residue-eutectic solvent solution immobilized at 300 kPa during the above process. CO2 ) data, in addition Figure 3 The appearance of films formed by mixing chitosan quaternary ammonium salt (CSQA), polyvinyl alcohol (PVA), eutectic solvent (DES), choline chloride-oxalic acid, and acetic acid residue-eutectic solvent (DES) solutions is compared. The results in the two figures show that the eutectic solvent (DES) does indeed play the roles of solvent, CO2 carrier, and film-forming aid at the same time.
[0031] Example 3
[0032] The eutectic solvent choline chloride-lactic acid (molar ratio 1:2) was mixed with defatted vinegar residue at a solid-liquid ratio of 1:10 (g / mL). The mixture was heated at 60℃ for 3 hours and filtered to obtain a choline chloride-lactic acid solution of the vinegar residue. CO2 was injected into the vinegar residue-eutectic solvent solution at a pressure of 600 kPa for 2 hours. Chitosan quaternary ammonium salt and polyvinyl alcohol were mixed at a solid-liquid ratio of 0.4:1 (g / mL), and deionized water was gradually added while heating and stirring at 40℃ until just dissolved to obtain a saturated solution of both. The vinegar residue solution with CO2 immobilized was then added to the saturated solution at a liquid-liquid ratio of 0.5:1 (mL / mL) and mixed thoroughly. The membrane slurry was poured onto a smooth and clean glass surface and allowed to flow naturally with a thickness controlled at 0.6 mm. After standing and air drying for 48 hours, the product dressing membrane was obtained. After sterilization by irradiation with ultraviolet light for 60 minutes, it was dispensed, sealed, and stored.
[0033] Example 4
[0034] The eutectic solvent citric acid-wood alcohol-water (molar ratio 1:1:10) was mixed with the defatted vinegar residue at a solid-liquid ratio of 1:8 (g / mL) and heated at 80°C for 3h. After filtration, the citric acid-wood alcohol-water dissolution solution of the vinegar residue was obtained. CO2 was pressurized into the vinegar residue-eutectic solvent dissolution solution at a pressure of 500kPa, and the pressurization time was 1.5h. The chitosan quaternary ammonium salt and polyvinyl alcohol were mixed at a solid-liquid ratio of 0.8:1 (g / mL), then deionized water was gradually added and heated and stirred at 40°C until just dissolved, thereby obtaining a saturated solution of the two. The CO2-loaded vinegar residue dissolution solution was added to the saturated solution at a liquid-liquid ratio of 0.6:1 (mL / mL) and mixed uniformly. The membrane slurry was poured onto a smooth and clean glass plane, and the thickness was controlled to be 0.8mm after natural flow. After standing and air-drying for 36 hours, the product dressing film was obtained. After sterilization under ultraviolet light for 60min, it was packaged and stored in a sealed manner.
[0035] Example 5
[0036] The eutectic solvent tetrabutylammonium chloride-oxalic acid (molar ratio 1:1) was mixed with the defatted vinegar residue at a solid-liquid ratio of 1:20 (g / mL) and heated at 80°C for 3h. After filtration, the tetrabutylammonium chloride-oxalic acid dissolution solution of the vinegar residue was obtained. CO2 was pressurized into the vinegar residue-eutectic solvent dissolution solution at a pressure of 200kPa, and the pressurization time was 2h. The chitosan quaternary ammonium salt and polyvinyl alcohol were mixed at a solid-liquid ratio of 0.4:1 (g / mL), then deionized water was gradually added and heated and stirred at 40°C until just dissolved, thereby obtaining a saturated solution of the two. The CO2-loaded vinegar residue dissolution solution was added to the saturated solution at a liquid-liquid ratio of 1.2:1 (mL / mL) and mixed uniformly. The membrane slurry was poured onto a smooth and clean glass plane, and the thickness was controlled to be 0.8mm after natural flow. After standing and air-drying for 42 hours, the product dressing film was obtained. After sterilization under ultraviolet light for 60min, it was packaged and stored in a sealed manner.
[0037] Example 6
[0038] The eutectic solvent ethylene glycol-oxalic acid (molar ratio 1:1) was mixed with the defatted vinegar residue at a solid-liquid ratio of 1:20 (g / mL) and heated at 60°C for 2 h. The ethylene glycol-oxalic acid solution of the vinegar residue was obtained after filtration. CO2 was pressurized into the vinegar residue-eutectic solvent solution at a pressure of 600 kPa, and the pressurization time was 1 h. Chitosan quaternary ammonium salt and polyvinyl alcohol were mixed at a solid-liquid ratio of 0.9:1 (g / mL), and then deionized water was gradually added and heated and stirred at 40°C until just dissolved, thereby obtaining a saturated solution of the two. The CO2-loaded vinegar residue solution was added to the saturated solution at a liquid-liquid ratio of 1.2:1 (mL / mL) and mixed uniformly. The membrane slurry was poured onto a smooth and clean glass plane, and the thickness was controlled to be 0.5 mm after natural flow. The product dressing film was obtained after standing and air-drying for 18 hours. After sterilization under ultraviolet light for 60 min, the product was packaged and stored in a sealed manner.
[0039] Example 7
[0040] The eutectic solvent 1,4-butanediol-oxalic acid (molar ratio 1:1) was mixed with the defatted vinegar residue at a solid-liquid ratio of 1:40 (g / mL) and heated at 60°C for 2 h. The 1,4-butanediol-oxalic acid solution of the vinegar residue was obtained after filtration. CO2 was pressurized into the vinegar residue-eutectic solvent solution at a pressure of 600 kPa, and the pressurization time was 1 h. Chitosan quaternary ammonium salt and polyvinyl alcohol were mixed at a solid-liquid ratio of 0.9:1 (g / mL), and then deionized water was gradually added and heated and stirred at 40°C until just dissolved, thereby obtaining a saturated solution of the two. The CO2-loaded vinegar residue solution was added to the saturated solution at a liquid-liquid ratio of 1.2:1 (mL / mL) and mixed uniformly. The membrane slurry was poured onto a smooth and clean glass plane, and the thickness was controlled to be 0.7 mm after natural flow. The product dressing film was obtained after standing and air-drying for 18 hours. After sterilization under ultraviolet light for 60 min, the product was packaged and stored in a sealed manner.
[0041] Example 8
[0042] The eutectic solvent 1,4-butanediol-oxalic acid (molar ratio 1:1) was mixed with the defatted vinegar residue at a solid-liquid ratio of 1:20 (g / mL) and uniformly heated at 90°C for 1.5 h. After filtration, the 1,4-butanediol-oxalic acid dissolution solution of the vinegar residue was obtained. CO2 was pressurized into the vinegar residue-eutectic solvent dissolution solution at a pressure of 500 kPa, and the pressurization time was 1.5 h. Chitosan quaternary ammonium salt and polyvinyl alcohol were mixed at a solid-liquid ratio of 0.6:1 (g / mL), and then deionized water was gradually added and heated and stirred at 40°C until just dissolved, thereby obtaining a saturated solution of the two. The CO2-loaded vinegar residue dissolution solution was added to the saturated solution at a liquid-liquid ratio of 1:1 (mL / mL) and mixed uniformly. The membrane paste was poured onto a smooth and clean glass plane, and the thickness was controlled to be 0.7 mm after natural flow. After standing and air-drying for 38 h, the product dressing film was obtained. After sterilization under ultraviolet lamp irradiation for 60 min, the product was packaged and stored.
[0043] Example 9
[0044] The eutectic solvent choline chloride-oxalic acid (molar ratio 1:1) was mixed with the defatted vinegar residue at a solid-liquid ratio of 1:20 (g / mL) and uniformly heated at 80°C for 2 h. After filtration, the choline chloride-oxalic acid dissolution solution of the vinegar residue was obtained. CO2 was pressurized into the vinegar residue-eutectic solvent dissolution solution at a pressure of 600 kPa, and the pressurization time was 2 h. Chitosan quaternary ammonium salt and polyvinyl alcohol were mixed at a solid-liquid ratio of 0.6:1 (g / mL), and then deionized water was gradually added and heated and stirred at 40°C until just dissolved, thereby obtaining a saturated solution of the two. The CO2-loaded vinegar residue dissolution solution was added to the saturated solution at a liquid-liquid ratio of 1:1 (mL / mL) and mixed uniformly. The membrane paste was poured onto a smooth and clean glass plane, and the thickness was controlled to be 0.5 mm after natural flow. After standing and air-drying for 48 h, the product dressing film was obtained. After sterilization under ultraviolet lamp irradiation for 60 min, the product was packaged and stored.
[0045] Example 10
[0046] The eutectic solvent (molar ratio 1:1) was mixed with defatted vinegar residue at a solid-liquid ratio of 1:20 (g / mL) and heated at 90°C for 2h, and after filtration, the choline chloride-oxalic acid dissolution solution of vinegar residue was obtained; CO2 was pressurized into the vinegar residue-eutectic solvent dissolution solution at a pressure of 400kPa, and the pressurization time was 2h; chitosan quaternary ammonium salt and polyvinyl alcohol were mixed according to a solid-liquid ratio of 0.7:1 (g / mL), then deionized water was gradually added and heated and stirred at 40°C until just dissolved, thereby obtaining a saturated solution of the two; then the CO2 immobilized vinegar residue dissolution solution was added to the saturated solution according to a liquid-liquid ratio of 0.8:1 (mL / mL) and mixed uniformly; the membrane paste was poured onto a smooth and clean glass plane, and the thickness was controlled to be 0.8mm after natural flow; after standing and air drying for 48 hours, the product dressing film was obtained, and after sterilization under ultraviolet lamp irradiation for 60min, it was packaged and stored.
[0047] For the nursing dressing product of Example 2, preliminary mechanical property and adhesion tests were performed, and the results are shown in Figure 4 and Figure 5 It can be seen from the Figure 4 results that the composite dressing film can not be broken when stretched to a certain length under the application of external force, and can immediately return to the initial state after being twisted, indicating that the composite dressing film has a certain mechanical strength and can meet the application requirements. In addition Figure 5 , the evaluation results show that the composite dressing film can be firmly and smoothly attached to different sample surfaces, including typical animal skin and other non-biological materials.
[0048] Since there is a link between calcium ion concentration and keratinocyte differentiation, which is reflected in the significant increase in calcium ion concentration before the differentiation of granular layer keratinocytes into stratum corneum keratinocytes, which helps to improve the role of stratum corneum repair. Therefore, according to the method in the literature (Lyubchenko T., et al., The Journal of Investigative Dermatology, 2021, 141(7): 1792-1801), the calcium ion chelating ability of the nursing dressing product obtained in Example 9 was determined, and the results are shown in Figure 6 It can be seen that as the amount of vinegar residue-eutectic solvent dissolution solution increases, the calcium ion chelating ability also gradually increases; this indicates that the vinegar residue-eutectic solvent dissolution solution contains rich calcium ion chelating agents or components that can form stable complexes with calcium ions, and this increase in chelating ability helps to regulate the release rate and concentration of calcium ions in specific applications, thereby optimizing its role in repairing the stratum corneum of the skin.
[0049] For the nursing dressing product obtained in Example 9, an antioxidant activity test was performed according to the method in the literature (Zhang Hao, et al. Gansu Science and Technology, 2008, 6:57-58), and the results are shown inFigure 7 As shown in the figure, the maximum DPPH scavenging rate is 60.3%, and the scavenging rate after 6h remains in a relatively stable stage, which is conducive to maintaining a relatively long-term care effect.
[0050] The care dressing product obtained in Example 9 was tested for tyrosinase inhibitory activity according to the method of national standard T / GDCA 006-2021. Tyrosinase is a redox enzyme that plays a key role in the synthesis of melanin. Inhibiting the activity of tyrosinase can reduce the production of melanin, which helps to prevent the formation and deterioration of pigmentation. The experimental results are shown in the figure Figure 8 As can be seen from the figure, as the amount of vinegar residue-hydrogen bond solvent solution added increases, the activity of the composite dressing film to inhibit tyrosinase also gradually increases, indicating that the active ingredients present in the vinegar residue-hydrogen bond solvent solution exhibit a clear dose-effect relationship.
Claims
1. A cosmetic care dressing taking vinegar residue, deep eutectic solvent and CO2 as core raw materials, comprising vinegar residue component, deep eutectic solvent, CO2 and other auxiliary materials.
2. The cosmetic care dressing of claim 1, wherein the core material is composed of vinegar residue, deep eutectic solvent, and CO2. The vinegar residue used is from the waste of the brewing industry, which is first degreased with petroleum ether and then dissolved with deep eutectic solvent to obtain a vinegar residue-deep eutectic solvent solution. 3.The cosmetic care dressing with vinegar residue, deep eutectic solvent and CO2 as core raw materials according to claim 1, characterized in that, The deep eutectic solvent includes choline chloride-oxalic acid, choline chloride-lactic acid, citric acid-xylose alcohol-water, tetrabutylammonium chloride-oxalic acid, ethylene glycol-oxalic acid, 1,4-butanediol-oxalic acid, which are used as vinegar residue component solvent, CO2 carrier and film-forming aid.
4. The cosmetic care dressing of claim 1, wherein the core material is composed of vinegar residue, deep eutectic solvent, and CO2. CO2 is used as an auxiliary functional material and is pressurized into the vinegar residue-deep eutectic solvent solution.
5. The cosmetic care dressing of claim 1, wherein the core material is composed of vinegar residue, deep eutectic solvent, and CO2. Other auxiliary materials are polyvinyl alcohol and chitosan quaternary ammonium salt.
6. The cosmetic care dressing of claim 1, wherein the core material is composed of vinegar residue, deep eutectic solvent, and CO2. The specific steps for preparing the dressing are as follows: (1) The vinegar residue is degreased with petroleum ether with a boiling range of 30-60℃ at a solid-liquid ratio of 1:1 (g / mL), heated at 30-60℃ for 2-3 h, filtered and dried to obtain degreased vinegar residue; (2) The degreased vinegar residue is mixed with the above-mentioned deep eutectic solvent at a solid-liquid ratio of 1:5-1:40 (g / mL), heated at 40-90℃ for 1-3 h, filtered and collected to obtain a vinegar residue-deep eutectic solvent solution; (3) CO2 is pressurized into the vinegar residue-deep eutectic solvent solution at a pressure of 200-600 kPa for 1-6 h; (4) Chitosan quaternary ammonium salt and polyvinyl alcohol are mixed at a solid-solid ratio of 0.1:1-1:1 (g / g), deionized water is gradually added and heated and stirred at 40℃ until they are just dissolved to obtain a saturated solution of the two; then the CO2-loaded vinegar residue-deep eutectic solvent solution is added to the saturated solution at a liquid-liquid ratio of 0.6:1-1.6:1 (mL / mL) and mixed uniformly; (5) Pour all the film paste onto a smooth and clean glass plane, let it flow naturally and control the thickness to be 0.5-1 mm; (6) Let the film paste stand naturally and air dry for 18-48 h, then remove the film after complete solidification; (7) Cut the prepared dressing product into a certain specification, sterilize it under ultraviolet light for 60 min, then package and store it after sealing.