Reagent transfer device

Abstract

The invention provides a reagent transfer device. The reagent transfer device comprises a kit, the kit is provided with a plurality of containing cavities and a plurality of communicating holes, the communicating holes are formed in the bottom of the kit, and different containing cavities are communicated with at least one different communicating hole; the number of the sealing assemblies is multiple, and at least one sealing assembly is arranged in each communicating hole; the reagent needle mounting plate is arranged corresponding to the bottom of the kit; the driving assembly is in driving connection with the reagent needle mounting plate and / or the kit, so that the reagent needle mounting plate moves in the direction close to or away from the bottom of the kit relative to the kit; the reagent connectors are detachably arranged on the reagent needle mounting plate and move relative to the kit along with the reagent needle mounting plate, and at least one part of each reagent connector can extrude or penetrate through the sealing assembly to be communicated with the communicating hole. The problem that in the prior art, a reagent transfer device is poor in use performance is solved.

Description

Technical Field

[0001] This invention relates to the field of reagent testing equipment, and more specifically, to a reagent transfer device. Background Technology

[0002] Currently, sequencing systems require different reagents to flow through a flow cell in a specific order during sequencing. Therefore, a reagent transfer system is necessary. Most manufacturers transfer reagents from the reagent kit to the tubing using a long reagent needle inserted from above the reagent surface to the bottom of the liquid, aspirating the reagent through negative pressure. However, this method of reagent transfer not only results in significant reagent residue in the tubing and at the bottom of the reagent kit, but also makes it difficult to clean the reagent residue on the outer wall of the needle. Furthermore, this reagent transfer device is complex in structure, bulky, and has low space utilization.

[0003] Therefore, existing reagent transfer devices suffer from poor performance. Summary of the Invention

[0004] The main objective of this invention is to provide a reagent transfer device to solve the problem of poor performance of existing reagent transfer devices.

[0005] To achieve the above objectives, according to one aspect of the present invention, a reagent transfer device is provided, comprising: a reagent kit having a plurality of accommodating cavities and a plurality of communicating holes, the communicating holes being disposed at the bottom of the reagent kit, and different accommodating cavities communicating with at least one different communicating hole; a plurality of sealing components, with at least one sealing component disposed in each communicating hole; a reagent needle mounting plate disposed corresponding to the bottom of the reagent kit; a driving component drivingly connected to the reagent needle mounting plate and / or the reagent kit to cause the reagent needle mounting plate to move relative to the reagent kit in a direction approaching or away from the bottom of the reagent kit; and a plurality of reagent connectors, the reagent connectors being detachably disposed on the reagent needle mounting plate and moving with the reagent needle mounting plate relative to the reagent kit, and at least a portion of the reagent connectors being capable of pressing or passing through the sealing components and communicating with the communicating holes.

[0006] Furthermore, the number of accommodating cavities, connecting holes, sealing components, and reagent connectors are the same and correspond one-to-one.

[0007] Furthermore, when the drive assembly drives the reagent needle mounting plate toward the bottom of the kit, the reagent connector squeezes the sealing assembly and causes at least a portion of the sealing assembly to move toward the receiving cavity.

[0008] Furthermore, the sealing assembly includes: a seal, wherein the circumferential inner wall of the end of the connecting hole away from the receiving cavity has a limiting protrusion, the seal being movably disposed at the end of the connecting hole having the limiting protrusion and abutting against the limiting protrusion; and a reset member, one end of the reset member abutting against the seal, and the other end of the reset member abutting against the reagent kit, to provide a reset force for the seal to move toward the limiting protrusion.

[0009] Furthermore, the reagent connector includes: a connecting section, at least a portion of which is detachably connected to a reagent needle mounting plate; and a liquid dispensing section disposed between the seal and the connecting section, the liquid dispensing section being connected to one end of the connecting section, the liquid dispensing section and the connecting section having a communicating liquid dispensing channel, wherein when the seal is squeezed, the liquid dispensing channel at one end of the liquid dispensing section communicates with a communicating hole.

[0010] Furthermore, the liquid-taking section includes: a body portion connected to the connecting section; and an extension portion disposed at the end of the body portion away from the connecting section. When the liquid-taking section extends into the connecting hole, the end of the body portion away from the connecting section abuts against the outer periphery of the connecting hole, and the extension portion extends into the connecting hole and squeezes the sealing element.

[0011] Furthermore, the liquid-taking section has a first liquid-taking channel inside, the connecting section has a second liquid-taking channel communicating with the first liquid-taking channel, the circumferential sidewall of the extending part has a liquid-taking hole communicating with the first liquid-taking channel; and / or the part of the main body corresponding to the communicating hole has a liquid-taking hole communicating with the first liquid-taking channel.

[0012] Furthermore, both the main body and the extension are cylindrical, the connecting hole, the main body, and the extension are concentric, and the diameter of the main body is larger than the diameter of the connecting hole, and the diameter of the connecting hole is larger than the diameter of the extension.

[0013] Furthermore, the reagent transfer device also includes multiple sealing rings, with at least one sealing ring provided around the periphery of each connecting hole.

[0014] Furthermore, the shape of the seal includes spherical, hemispherical, frustum-shaped, or conical.

[0015] Furthermore, the sealing assembly includes a rubber stopper, at least a portion of which is disposed inside the communication hole. When the drive assembly drives the reagent needle mounting plate toward the bottom of the kit, the end of the reagent connector away from the reagent needle mounting plate passes through the rubber stopper and extends into the communication hole.

[0016] Furthermore, the reagent connector includes: a connecting section, at least a portion of which is detachably connected to a reagent needle mounting plate; and a liquid dispensing section connected to the end of the connecting section away from the reagent needle mounting plate. The end of the liquid dispensing section away from the connecting section can pass through a rubber stopper and extend into the interior of a communicating hole. The interior of the liquid dispensing section has a first liquid dispensing channel, and the connecting section has a second liquid dispensing channel communicating with the first liquid dispensing channel. The end of the liquid dispensing section away from the connecting section has a liquid dispensing hole communicating with the first liquid dispensing channel, and the liquid dispensing section is needle-shaped and gradually contracts in the direction away from the connecting section.

[0017] Furthermore, the reagent transfer device also includes a support plate, on which the reagent kit is placed, and the support plate is provided with multiple clearance holes corresponding to multiple connecting holes.

[0018] Furthermore, the reagent transfer device also includes a limiting plate, which is positioned on the side of the reagent kit away from the reagent needle mounting plate and abuts against the reagent kit.

[0019] Furthermore, the drive assembly includes: a mounting plate; a guide rail, which is disposed on the mounting plate, and the reagent needle mounting plate is movably connected to the guide rail and can move along the guide rail; and a drive motor, which is drivenly connected to the reagent needle mounting plate.

[0020] Furthermore, the drive assembly also includes: a lead screw, to which the drive motor is connected; a nut, which is sleeved on the lead screw and can move along the lead screw, and which cooperates with the guide rail; and a fixing plate, to which the reagent needle mounting plate is connected to the nut.

[0021] Furthermore, the reagent needle mounting plate has multiple connecting tubes, with different reagent connectors connected to different connecting tubes. The ends of the multiple connecting tubes furthest from the reagent connectors converge at the liquid outlet of the reagent needle mounting plate. The reagent transfer device also includes a rotary valve, which is located at the liquid outlet and selectively connects the liquid outlet to any one of the multiple connecting tubes.

[0022] Applying the technical solution of this invention, the reagent transfer device in this application includes a reagent kit, a sealing assembly, a reagent needle mounting plate, a driving assembly, and reagent connectors. The reagent kit has multiple cavities and multiple communicating holes, with the communicating holes located at the bottom of the reagent kit, and different cavities communicating with at least one different communicating hole. Multiple sealing assemblies are provided, with at least one sealing assembly disposed within each communicating hole. The reagent needle mounting plate is disposed corresponding to the bottom of the reagent kit. The driving assembly is drivenly connected to the reagent needle mounting plate and / or the reagent kit, causing the reagent needle mounting plate to move relative to the reagent kit in a direction approaching or away from the bottom of the reagent kit. Multiple reagent connectors are provided, detachably mounted on the reagent needle mounting plate and moving relative to the reagent kit with the reagent needle mounting plate, and at least a portion of the reagent connector can squeeze or pass through the sealing assembly and communicate with the communicating hole.

[0023] When using the reagent transfer device of this application, the reagent to be transferred is placed in multiple cavities of the reagent kit. When reagent transfer is required, the reagent tubing is connected to the reagent connector or reagent needle mounting plate. The reagent needle mounting plate, driven by the drive assembly, moves the reagent connector toward the bottom of the reagent kit. When the reagent needle mounting plate is in position, the reagent connector squeezes or passes through the sealing assembly and communicates with the communication hole, thereby aspirating the reagent inside the cavities. In other words, in this application, reagent can be aspirated from the bottom of the reagent kit through the reagent connector, thus eliminating the need for a very long reagent needle to aspirate the reagent inside the kit. It also solves the problems of excessive reagent residue in the tubing and excessive reagent residue at the bottom of the kit. Therefore, the reagent transfer device of this application effectively solves the problem of poor performance of existing reagent transfer devices. Attached Figure Description

[0024] The accompanying drawings, which form part of this application, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an undue limitation of the invention. In the drawings:

[0025] Figure 1 A schematic diagram of the reagent transfer device in a specific embodiment of this application is shown;

[0026] Figure 2 It shows Figure 1 Enlarged view of point A in the middle;

[0027] Figure 3 A schematic diagram of the reagent transfer device in another specific embodiment of this application is shown;

[0028] Figure 4 It shows Figure 3 Enlarged view of point B in the middle;

[0029] Figure 5 This invention provides a schematic diagram showing the positional relationship between the reagent needle mounting plate and the rotary valve of the reagent transfer device in a specific embodiment of the present application.

[0030] Figure 6 This illustration shows a schematic diagram of the positional relationship between the drive assembly and the reagent needle mounting plate of a reagent transfer device in a specific embodiment of this application;

[0031] Figure 7 This illustration shows a schematic diagram of the positional relationship between the reagent connector and the sealing assembly when the reagent connector of the reagent transfer device is connected to the communication hole in a specific embodiment of this application;

[0032] Figure 8 This illustration shows a schematic diagram of the positional relationship between the reagent connector and the sealing assembly when the reagent connector of the reagent transfer device is not connected to the communication hole in a specific embodiment of this application;

[0033] Figure 9 A schematic diagram of the reagent needle mounting plate of a reagent transfer device in a specific embodiment of this application is shown.

[0034] The above figures include the following reference numerals:

[0035] 10. Reagent kit; 11. Receptacle; 12. Communicating hole; 20. Sealing assembly; 21. Seal; 22. Reset component; 23. Rubber stopper; 30. Reagent needle mounting plate; 31. Communicating tubing; 32. Liquid outlet; 40. Drive assembly; 41. Mounting plate; 42. Guide rail; 43. Drive motor; 44. Lead screw; 45. Nut; 46. Fixing plate; 50. Reagent connector; 51. Connecting section; 511. Second liquid dispensing channel; 52. Liquid dispensing section; 521. First liquid dispensing channel; 522. Liquid dispensing hole; 523. Body; 524. Extension part; 60. Sealing ring; 70. Support plate; 80. Rotary valve. Detailed Implementation

[0036] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0037] It should be noted that, unless otherwise specified, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0038] In this invention, unless otherwise stated, directional terms such as "upper," "lower," "top," and "bottom" are generally used in relation to the direction shown in the accompanying drawings, or in relation to the vertical, perpendicular, or gravitational direction of the component itself; similarly, for ease of understanding and description, "inner" and "outer" refer to the inner and outer contours of each component itself, but the above directional terms are not intended to limit this invention.

[0039] To address the problem of poor performance in existing reagent transfer devices, this application provides a reagent transfer device.

[0040] like Figures 1 to 9As shown, the reagent transfer device in this application includes a reagent kit 10, a sealing assembly 20, a reagent needle mounting plate 30, a driving assembly 40, and reagent connectors 50. The reagent kit 10 has multiple accommodating cavities 11 and multiple communicating holes 12, with the communicating holes 12 located at the bottom of the reagent kit 10, and different accommodating cavities 11 communicating with at least one different communicating hole 12; there are multiple sealing assemblies 20, with at least one sealing assembly 20 disposed within each communicating hole 12; the reagent needle mounting plate 30 is disposed corresponding to the bottom of the reagent kit 10; the driving assembly 40 is drivenly connected to the reagent needle mounting plate 30 and / or the reagent kit 10, so that the reagent needle mounting plate 30 moves relative to the reagent kit 10 in a direction approaching or away from the bottom of the reagent kit 10; there are multiple reagent connectors 50, each reagent connector 50 is detachably disposed on the reagent needle mounting plate 30 and moves with the reagent needle mounting plate 30, and at least a portion of the reagent connector 50 can squeeze or pass through the sealing assembly 20 and communicate with the communicating hole 12.

[0041] When using the reagent transfer device of this application, the reagent to be transferred is placed in multiple cavities 11 of the reagent kit 10. When reagent transfer is required, the reagent tubing is connected to the reagent connector 50 or the reagent needle mounting plate 30. Under the drive of the drive assembly 40, the reagent needle mounting plate 30 moves the reagent connector 50 toward the bottom of the reagent kit 10. When the reagent needle mounting plate 30 is in position, the reagent connector 50 squeezes or passes through the sealing assembly 20 and communicates with the communicating hole 12, thereby aspirating the reagent inside the cavities 11. In other words, in this application, reagent can be aspirated from the bottom of the reagent kit 10 through the reagent connector 50. Therefore, it is not necessary to use a very long reagent needle to aspirate the reagent in the reagent kit 10. At the same time, it can also solve the problems of excessive reagent residue in the tubing and excessive reagent residue at the bottom of the reagent kit 10. Therefore, the reagent transfer device of this application effectively solves the problem of poor performance of reagent transfer devices in the prior art.

[0042] In one specific embodiment of this application, the driving component 40 is driven to connect with the reagent needle mounting plate 30.

[0043] Preferably, the number of accommodating cavities 11, communicating holes 12, sealing components 20, and reagent connectors 50 are the same and correspond one-to-one. That is, in this application, each accommodating cavity 11 has a communicating hole 12 connected to its bottom, and a sealing component 20 is correspondingly provided inside the communicating hole 12. At the same time, the reagent connectors 50 on the reagent needle mounting plate 30 correspond to the communicating holes 12 in both position and number. Of course, in this application, the number of accommodating cavities 11, communicating holes 12, sealing components 20, and reagent connectors 50 can be adaptively adjusted, and the adjusted number of accommodating cavities 11, communicating holes 12, sealing components 20, and reagent connectors 50 may not be equal.

[0044] like Figure 1 and Figure 2 As shown, in one specific embodiment of this application, when the driving assembly 40 drives the reagent needle mounting plate 30 to move toward the bottom of the reagent kit 10, the reagent connector 50 squeezes the sealing assembly 20 and causes at least a portion of the sealing assembly 20 to move toward the receiving cavity 11. The sealing assembly 20 includes a sealing member 21 and a resetting member 22. The circumferential inner wall of the end of the connecting hole 12 away from the receiving cavity 11 has a limiting protrusion. The sealing member 21 is movably disposed at the end of the connecting hole 12 with the limiting protrusion and abuts against the limiting protrusion. One end of the resetting member 22 abuts against the sealing member 21, and the other end of the resetting member 22 abuts against the reagent kit 10, providing a resetting force for the sealing member 21 to move toward the limiting protrusion. With this configuration, when the reagent needle mounting plate 30 is not in position, the sealing member 21 can block the end of the connecting hole 12 away from the receiving cavity 11. After the reagent needle mounting plate 30 is in position, the reagent connector 50 can abut against the sealing member 21 and squeeze the sealing member 21, and the resetting member 22 is compressed. At this time, the seal 21 moves toward the receiving cavity 11, and the reagent connector 50 can extend into the connecting hole 12 to aspirate liquid.

[0045] Optionally, the seal is a rubber ball. Furthermore, in this application, the shape of the seal 21 can be any one of a sphere, hemisphere, frustum, or cone.

[0046] It should be noted that in this embodiment, the reset member 22 can be a spring, and the spring can be installed in a way that ensures that it can provide a reset force to the seal 21 toward the end of the connecting hole 12 away from the receiving cavity 11.

[0047] Specifically, the reagent connector 50 includes a connecting section 51 and a liquid-dispensing section 52 disposed between the sealing member 21 and the connecting section 51. At least a portion of the connecting section 51 is detachably connected to the reagent needle mounting plate 30; the liquid-dispensing section 52 is connected to the end of the connecting section 51 away from the reagent needle mounting plate 30, and the liquid-dispensing section 52 and the connecting section 51 have a communicating liquid-dispensing channel. When the sealing member 21 is squeezed, the liquid-dispensing channel at one end of the liquid-dispensing section 52 communicates with the communicating hole 12. The end of the liquid-dispensing section 52 away from the connecting section 51 can extend into the communicating hole 12 and squeeze the sealing assembly 20 toward the receiving cavity 11. The interior of the liquid-dispensing section 52 has a first liquid-dispensing channel 521, the connecting section 51 has a second liquid-dispensing channel 511 communicating with the first liquid-dispensing channel 521, and the end of the liquid-dispensing section 52 away from the connecting section 51 has a liquid-dispensing hole 522 communicating with the first liquid-dispensing channel 521. Preferably, the liquid-collecting section 52 includes a body portion 523 and an extension portion 524. The body portion 523 is connected to the connecting section 51. The extension portion 524 is located at the end of the body portion 523 away from the connecting section 51 and has a liquid-collecting hole 522. When the liquid-collecting section 52 extends into the connecting hole 12, the end of the body portion 523 away from the connecting section 51 abuts against the outer periphery of the connecting hole 12, and the extension portion 524 extends into the connecting hole 12 and compresses the sealing member 21. That is, in this embodiment, the liquid-collecting section 52 can enter the connecting hole 12. Of course, in this application, the body portion 523 may also have a liquid-collecting hole 522 corresponding to the connecting hole 12 that communicates with the first liquid-collecting channel 521.

[0048] Optionally, the reagent needle mounting plate is provided with a mounting hole corresponding to the reagent connector. The end of the connecting section away from the liquid dispensing section extends into the interior of the mounting hole and is threadedly connected to the circumferential inner sidewall of the mounting hole. The reagent tubing can extend into the second liquid dispensing channel of the connecting section and be threadedly connected to the connecting section.

[0049] Optionally, both the body portion 523 and the extension portion 524 are cylindrical, the connecting hole 12, the body portion 523, and the extension portion 524 are concentric, and the diameter of the body portion 523 is larger than the diameter of the connecting hole 12, and the diameter of the connecting hole 12 is larger than the diameter of the extension portion 524.

[0050] Optionally, the dispensing hole 522 is located on the circumferential sidewall of the insertion portion 524. Since the top of the reagent connector 50 abuts against and compresses the sealing assembly 20 in this embodiment, placing the dispensing hole 522 on the circumferential sidewall of the insertion portion 524 ensures that the reagent in the accommodating cavity 11 can more easily enter the dispensing hole 522 through the connecting hole 12. Of course, the specific location of the dispensing hole 522 can also be adjusted according to actual usage.

[0051] Preferably, the reagent transfer device further includes multiple sealing rings 60, with at least one sealing ring 60 disposed around the periphery of each connecting hole 12. This arrangement effectively prevents leakage during reagent transfer.

[0052] Therefore, in this embodiment, a structure similar to a one-way valve is mainly formed by the sealing component 20 and the connecting hole 12 to achieve negative pressure liquid suction.

[0053] like Figure 3 and Figure 4 As shown, in another specific embodiment of this application, the sealing assembly 20 includes a rubber plug 23, at least a portion of which is disposed inside the communicating hole 12. When the driving assembly 40 drives the reagent needle mounting plate 30 to move toward the bottom of the reagent kit 10, the end of the reagent connector 50 away from the reagent needle mounting plate 30 passes through the rubber plug 23 and extends into the communicating hole 12. Furthermore, the reagent connector 50 includes a connecting section 51 and a liquid-dispensing section 52. At least a portion of the connecting section 51 is detachably connected to the reagent needle mounting plate 30. The liquid-dispensing section 52 is connected to the end of the connecting section 51 away from the reagent needle mounting plate 30. The end of the liquid-dispensing section 52 away from the connecting section 51 can pass through the rubber stopper 23 and extend into the interior of the connecting hole 12. The interior of the liquid-dispensing section 52 has a first liquid-dispensing channel 521. The connecting section 51 has a second liquid-dispensing channel 511 communicating with the first liquid-dispensing channel 521. The end of the liquid-dispensing section 52 away from the connecting section 51 has a liquid-dispensing hole 522 communicating with the first liquid-dispensing channel 521. The liquid-dispensing section 52 is needle-shaped and gradually tapers away from the connecting section 51. This design aims to ensure that the reagent connector 50 can more easily pierce the rubber stopper 23 and communicate with the connecting hole 12. Of course, in this embodiment, the reagent connector 50 can also be a reagent needle.

[0054] In this application, the reagent transfer device also includes a support plate 70, on which the reagent kit 10 is disposed, and the support plate 70 has multiple clearance holes corresponding to the multiple connecting holes 12. Furthermore, the reagent transfer device also includes a limiting plate, which is disposed on the side of the reagent kit 10 away from the reagent needle mounting plate 30 and abuts against the reagent kit 10. The support plate 70 and the limiting plate effectively position the reagent kit 10. Simultaneously, the limiting plate prevents the reagent connector 50 from being pushed up during compression or passage through the sealing assembly 20, thereby ensuring the performance of the reagent transfer device in this application. Of course, in this application, when the weight of the reagent kit is sufficiently large, the limiting plate may not be required.

[0055] Specifically, the drive assembly 40 includes a mounting plate 41, a guide rail 42, and a drive motor 43. The guide rail 42 is disposed on the mounting plate 41, and the reagent needle mounting plate 30 is movably connected to the guide rail 42 and can move along the guide rail 42; the drive motor 43 is drivenly connected to the reagent needle mounting plate 30. Of course, in this application, the drive assembly 40 can also be composed of other structures, as long as the drive assembly 40 can drive the reagent needle mounting plate 30 to move in a direction close to or away from the bottom of the reagent kit 10.

[0056] Optionally, the drive assembly 40 further includes: a lead screw 44, to which the drive motor 43 is driven; a nut 45, which is sleeved on the lead screw 44 and can move along the lead screw 44, and which cooperates with the guide rail 42; and a fixing plate 46, to which the reagent needle mounting plate 30 is connected to the nut 45. With this arrangement, the drive motor 43 can drive the lead screw 44 to rotate, and when the lead screw 44 rotates, the nut 45 can move along the lead screw 44, thereby driving the reagent needle mounting plate 30 to move. The cooperation between the nut 45 and the guide rail 42 allows the guide rail 42 to limit the movement of the nut 45.

[0057] Optionally, the reagent needle mounting plate 30 has multiple connecting tubes 31, and different reagent connectors 50 are connected to different connecting tubes 31 respectively. The ends of the multiple connecting tubes 31 away from the reagent connectors all converge at the outlet 32 ​​of the liquid outlet end of the reagent needle mounting plate 30. The reagent transfer device also includes a rotary valve 80, which is disposed at the outlet 32 ​​and selectively connects the outlet 32 ​​to any one of the multiple connecting tubes 31.

[0058] As can be seen from the above description, the embodiments of the present invention achieve the following technical effects:

[0059] 1. Effectively solves the problem of poor performance of reagent transfer devices in existing technologies;

[0060] 2. Simple structure and stable performance.

[0061] Obviously, the embodiments described above are merely some, not all, embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort should fall within the scope of protection of the present invention.

[0062] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0063] It should be noted that the terms "first," "second," etc., used in the specification, claims, and accompanying drawings of this application are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence. It should be understood that such data can be interchanged where appropriate so that the embodiments of this application described herein can be implemented in sequences other than those illustrated or described herein.

[0064] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A reagent transfer device, characterized by, The kit (10) has a plurality of accommodation cavities (11) and a plurality of communication holes (12), the communication holes (12) are arranged at the bottom of the kit (10), and different accommodation cavities (11) are communicated with at least one different communication hole (12); A sealing assembly (20) is arranged in each communication hole (12); A reagent needle mounting plate (30) is arranged at the bottom of the reagent kit (10); A driving assembly (40) is drivingly connected with the reagent needle mounting plate (30) and / or the reagent kit (10) to drive the reagent needle mounting plate (30) to move relative to the reagent kit (10) in the direction of approaching or moving away from the bottom of the reagent kit (10); A plurality of reagent connectors (50) are detachably arranged on the reagent needle mounting plate (30) and move with the reagent needle mounting plate (30) relative to the reagent kit (10), and at least a part of the reagent connector (50) can extrude or pass through the sealing assembly (20) and communicate with the communication hole (12). The number of the accommodation cavities (11), the communication holes (12), the sealing assemblies (20) and the reagent connectors (50) is the same and one-to-one correspondence.

2. The reagent transfer device of claim 1, wherein, When the driving assembly (40) drives the reagent needle mounting plate (30) to move towards the bottom of the reagent kit (10), the reagent connector (50) extrudes the sealing assembly (20) and moves at least a part of the sealing assembly (20) towards the accommodation cavity (11).

3. The reagent transfer device of claim 1, wherein, The sealing assembly (20) comprises:

4. The reagent transfer device of claim 3, wherein, A sealing member (21) is movably arranged at one end of the communication hole (12) having the limiting protrusion and abuts against the limiting protrusion; A reset member (22) abuts against the sealing member (21) at one end and abuts against the reagent kit (10) at the other end to provide a reset force for the sealing member (21) to move towards the limiting protrusion. The reagent connector (50) comprises:

5. The reagent transfer device of claim 4, wherein, A connecting section (51) is detachably connected with the reagent needle mounting plate (30) at least in part; A liquid taking section (52) is arranged between the sealing member (21) and the connecting section (51), the liquid taking section (52) is connected with one end of the connecting section (51), the liquid taking section (52) and the connecting section (51) have a communication liquid taking channel, when the sealing member (21) is extruded, the liquid taking channel is in communication with one end of the liquid taking section (52) and the communication hole (12). The liquid taking section (52) comprises:

6. The reagent transfer device of claim 5, wherein, A body part (523) is connected with the connecting section (51); ​ The extension part (524) is arranged at one end of the body part (523) away from the connecting section (51), and when the liquid taking section (52) extends into the communication hole (12), the one end of the body part (523) away from the connecting section (51) abuts against the outer periphery of the communication hole (12), and the extension part (524) extends into the communication hole (12) and presses the sealing member (21).

7. The reagent transfer device of claim 6, wherein, The liquid taking section (52) has a first liquid taking channel (521) inside, and the connecting section (51) has a second liquid taking channel (511) in communication with the first liquid taking channel (521), The circumferential side wall of the extension part (524) has a liquid taking hole (522) in communication with the first liquid taking channel (521); and / or The part of the body part (523) corresponding to the communication hole (12) has a liquid taking hole (522) in communication with the first liquid taking channel (521).

8. The reagent transfer device of claim 6, wherein, The body part (523) and the extension part (524) are both cylindrical, the communication hole (12), the body part (523), and the extension part (524) are concentric, and the diameter of the body part (523) is greater than the diameter of the communication hole (12), and the diameter of the communication hole (12) is greater than the diameter of the extension part (524).

9. The reagent transfer device of claim 6, wherein, The reagent transfer device further comprises a plurality of sealing rings (60), and at least one sealing ring (60) is arranged at the periphery of each communication hole (12).

10. The reagent transfer device of claim 4, wherein, The shape of the sealing member (21) includes a sphere, a hemisphere, a circular truncated cone, or a cone.

11. The reagent transfer device of claim 1, wherein, The sealing assembly (20) comprises a rubber plug (23), at least a part of the rubber plug (23) is arranged inside the communication hole (12), and when the driving assembly (40) drives the reagent needle mounting plate (30) to move towards the bottom of the reagent box (10), the end of the reagent connector (50) away from the reagent needle mounting plate (30) penetrates through the rubber plug (23) and extends into the inside of the communication hole (12).

12. The reagent transfer device of claim 11, wherein, The reagent connector (50) comprises: A connecting section (51), at least a part of the connecting section (51) is detachably connected with the reagent needle mounting plate (30); A liquid taking section (52), the liquid taking section (52) is connected with the end of the connecting section (51) away from the reagent needle mounting plate (30), the end of the liquid taking section (52) away from the connecting section (51) can penetrate through the rubber plug (23) and extend into the inside of the communication hole (12), the liquid taking section (52) has a first liquid taking channel (521) inside, the connecting section (51) has a second liquid taking channel (511) in communication with the first liquid taking channel (521), the end of the liquid taking section (52) away from the connecting section (51) has a liquid taking hole (522) in communication with the first liquid taking channel (521), and the liquid taking section (52) is needle-shaped and gradually shrinks in the direction away from the connecting section (51).

13. The reagent transfer device of any one of claims 1 to 12, wherein, The reagent transfer device further comprises a bearing plate (70), the reagent box (10) is arranged on the bearing plate (70), and the bearing plate (70) is provided with a plurality of avoiding holes corresponding to the plurality of communication holes (12).

14. The reagent transfer device of any one of claims 1 to 12, wherein, The reagent transfer device further comprises a limiting plate, which is arranged on the side of the reagent box (10) away from the reagent needle mounting plate (30) and abuts against the reagent box (10).

15. The reagent transfer device of any one of claims 1 to 12, wherein, The driving assembly (40) comprises: a mounting plate (41); a guide rail (42) arranged on the mounting plate (41), the reagent needle mounting plate (30) is movably connected with the guide rail (42) and can move along the guide rail (42); a driving motor (43) drivingly connected with the reagent needle mounting plate (30).

16. The reagent transfer device of claim 15, wherein, The driving assembly (40) further comprises: a lead screw (44), the driving motor (43) is drivingly connected with the lead screw (44); a nut (45) sleeved on the lead screw (44) and capable of moving along the lead screw (44), and the nut (45) is matched with the guide rail (42); a fixed plate (46), the reagent needle mounting plate (30) is connected with the nut (45) through the fixed plate (46).

17. The reagent transfer device of any one of claims 1 to 12, wherein, The reagent needle mounting plate (30) has a plurality of communication pipelines (31), different reagent connectors (50) are respectively communicated with different communication pipelines (31), and the ends of the plurality of communication pipelines (31) away from the reagent connectors are all converged at the liquid outlet (32) of the liquid outlet end of the reagent needle mounting plate (30), and the reagent transfer device further comprises a rotary valve (80), which is arranged at the liquid outlet (32) and selectively communicates the liquid outlet (32) with any one of the plurality of communication pipelines (31).

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