Anaerobic ammonium oxidation bacteria culture equipment and culture method

Abstract

The invention discloses anaerobic ammonium oxidation bacteria culture equipment and a culture method, and relates to the field of microbial culture.The anaerobic ammonium oxidation bacteria culture equipment comprises a tank body, the tank body comprises an outer tank and an inner tank arranged in the outer tank, and the inner tank is provided with a water inlet pipe used for injecting nutrient solution and a strain inoculation pipe used for injecting strains; the inner tank and the outer tank are communicated through a water passing hole formed in the bottom of the inner tank, an overflow device is arranged at the top of the outer tank, a bacterium discharging pipe is arranged at the bottom of the outer tank, the overflow device is lower than the water inlet pipe, and a tank cover is arranged at the top of the tank body. Through the structure that the inner tank serves as a buffer tank and an adaptive tank, the problem that oxygen is contained in a nutrient solution added for the first time is solved, meanwhile, the problem that the environment is unstable is solved, the problem that the environmental stability is easily affected when waste liquid is discharged is further solved through the arrangement of the overflow device, and the stable growth environment is provided for anaerobic ammonium oxidation bacteria; the growth, reproduction and stability of bacteria are facilitated.

Description

Technical Field

[0001] This invention relates to the field of microbial culture, and in particular to an anaerobic ammonia-oxidizing bacteria culture device and culture method. Background Technology

[0002] Anaerobic ammonia oxidizing bacteria do not require an external carbon source or oxygen during denitrification, and produce little sludge. As a highly efficient and energy-saving microorganism, it has significant technical advantages in treating landfill leachate with high nitrogen content.

[0003] However, the generation time (time required for one division) of anaerobic ammonia oxidizing bacteria is as long as 10-14 days, which means that once the system collapses or the strain is lost, recovery takes a very long time. At the same time, anaerobic ammonia oxidizing bacteria are sensitive to changes in the external environment, and the growth environment needs to be precisely controlled, which is difficult to manage. Therefore, it is necessary to provide a stable environment suitable for the growth and reproduction of anaerobic ammonia oxidizing bacteria to ensure their rapid and large-scale reproduction.

[0004] Currently, existing equipment for culturing anaerobic ammonia oxidizing bacteria cannot achieve large-scale cultivation. There is an urgent need to develop anaerobic ammonia oxidizing bacteria cultivation equipment and methods to create a suitable growth environment for anaerobic ammonia oxidizing bacteria, thereby enabling large-scale cultivation. Summary of the Invention

[0005] The purpose of this invention is to provide an anaerobic ammonia oxidizing bacteria cultivation device and cultivation method to solve the problems existing in the prior art, provide a stable production environment for anaerobic ammonia oxidizing bacteria, and thus realize the large-scale cultivation of anaerobic ammonia oxidizing bacteria.

[0006] To achieve the above objectives, the present invention provides the following solution: This invention provides an anaerobic ammonia-oxidizing bacteria cultivation device, comprising: a tank body, the tank body including an outer tank and an inner tank disposed inside the outer tank, the inner tank being provided with a water inlet pipe for injecting nutrient solution and a bacterial inoculation pipe for injecting bacterial strains, the inner tank and the outer tank being connected through a water passage hole located at the bottom of the inner tank, the outer tank being provided with an overflow device at the top and a bacterial discharge pipe at the bottom, the overflow device being lower than the water inlet pipe, and a tank cover being provided at the top of the tank body.

[0007] As one embodiment, the overflow device includes an overflow weir and an overflow pipe for discharging waste liquid. The overflow weir is connected to the outlet pipe, and the overflow pipe is installed at a height higher than the overflow weir.

[0008] As one implementation, the overflow weir is sawtooth shaped.

[0009] As one embodiment, the top of the inner tank is also provided with a central tube for guiding the flow. The water outlet of the inner tank is located at the upper end of the central tube. The inoculation tube extends from the upper end of the central tube to the lower part of the central tube. The lower end of the central tube is an inverted water-absorbing funnel-shaped structure.

[0010] As one embodiment, the outer tank is also equipped with a composite monitor for measuring the temperature, pH and dissolved oxygen of the nutrient solution, and the top of the inner tank is also equipped with a dosing pipe for adding other drugs.

[0011] As one embodiment, the outer wall of the outer tank is also provided with a plurality of sampling tubes for sampling at vertical intervals.

[0012] As one embodiment, valves are provided at the external outlets of the above-mentioned discharge tube, the inoculation tube, and the sampling tube.

[0013] As one embodiment, both the inner tank and the bottom of the outer tank are equipped with submersible mixers for promoting the mixing of nutrient solution and bacterial strains.

[0014] As one embodiment, the tank cover is provided with a lifting hole, which serves as a channel for removing or placing the submersible mixer into the tank when it needs to be replaced or repaired.

[0015] The present invention also provides a method for culturing anaerobic ammonia-oxidizing bacteria, using the anaerobic ammonia-oxidizing bacteria culture equipment described above, comprising the following steps: S1: A fixed amount of nutrient solution is introduced into the inner tank through the water inlet pipe; S2: A quantitative amount of facultative anaerobic bacteria is introduced into the inner tank through the inoculation tube, and after a set time; S3: A quantitative amount of bacteria is inoculated through a bacterial inoculation tube over a set time. S4: Bacterial discharge is carried out through the discharge tube.

[0016] The present invention achieves the following technical effects compared to the prior art: This invention employs a fermentation structure consisting of an inner tank and an outer tank. Firstly, the inner tank acts as a buffer. When new nutrient solution is added, the facultative anaerobic bacteria within consume the small amount of oxygen in the newly added solution, ensuring that the nutrient solution entering the outer tank through the water passage remains anaerobic. This provides a stable, anaerobic growth environment for the anaerobic ammonia-oxidizing bacteria. Secondly, the inner tank acts as an adaptation tank. In the low-oxygen environment of the inner tank, the anaerobic ammonia-oxidizing bacteria undergo dormancy. Once the facultative anaerobic bacteria consume the small amount of oxygen in the inner tank, the anaerobic bacteria multiply rapidly. This invention solves both the problem of oxygen present in the initial nutrient solution addition and the problem of environmental changes affecting the growth and reproduction of anaerobic ammonia-oxidizing bacteria when new nutrient solution is added. The overflow device further addresses the issue of environmental instability during wastewater discharge. This invention provides a stable growth environment for anaerobic ammonia-oxidizing bacteria, contributing to their growth, reproduction, and stability. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is an overall structural diagram of the anaerobic ammonia-oxidizing bacteria culture equipment of the present invention.

[0019] The components are as follows: 1. Inner tank; 2. Outer tank; 3. Water inlet pipe; 4. Central pipe; 5. Inoculation pipe; 6. Dosing pipe; 7. Water passage hole; 8. Submersible mixer; 9. Composite monitoring instrument; 10. Overflow weir; 11. Water outlet pipe; 12. Sampling pipe; 13. Manhole; 14. Discharge pipe; 15. Overflow pipe; 16. Valve; 17. Tank lid; 18. Exhaust pipe; 19. Railing; 20. Electric hoist; 21. Lifting hole. Detailed Implementation

[0020] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] The purpose of this invention is to provide an anaerobic ammonia oxidizing bacteria cultivation device and method to solve the problems existing in the prior art, provide a stable growth environment for anaerobic ammonia oxidizing bacteria, and thus realize the large-scale cultivation of anaerobic ammonia oxidizing bacteria.

[0022] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0023] like Figure 1 As shown, the present invention provides an anaerobic ammonia-oxidizing bacteria cultivation device, including a tank body, which is divided into an outer tank 2 and an inner tank 1. The inner tank 1 is located inside the outer tank 2. The inner tank 1 is equipped with a water inlet pipe 3 and a bacterial inoculation pipe 5. The nutrient solution for cultivating the bacterial strain is injected into the inner tank 1 through the water inlet pipe 3. The bacterial strains that need to be cultivated or reacted are injected into the inner tank 1 through the bacterial inoculation pipe 5. A water passage hole 7 is located at the bottom of the inner tank 1. The inner tank 1 and the outer tank 2 are connected through the water passage hole 7. The inner tank 1 and the outer tank 2 form a structure similar to a communicating vessel. When the nutrient solution is added for the first time, according to the principle of communicating vessels, the liquid level of the nutrient solution in the inner tank 1 and the outer tank 2 is the same.

[0024] In use, the nutrient solution is added to the inner tank 1 through the water inlet pipe 3. The prepared facultative anaerobic bacteria are then introduced into the inner tank 1 through the inoculation pipe 5. A small number of facultative anaerobic bacteria will enter the outer tank 2 through the water outlet 7. Since this is an aerobic environment, the facultative anaerobic bacteria multiply rapidly, consuming the small amount of oxygen in the nutrient solution of both the inner tank 1 and the outer tank 2. After a period of time, the facultative anaerobic bacteria have consumed all the oxygen in both the inner tank 1 and the outer tank 2. At this point, both the inner tank 1 and the outer tank 2 are in an anaerobic state, and the reproduction of facultative anaerobic bacteria is inhibited. Anaerobic ammonia oxidizing bacteria are then introduced through the inoculation pipe 5, and the anaerobic ammonia oxidizing bacteria multiply rapidly in the anaerobic environment. After a period of time, new nutrient solution needs to be added to the tank. This new nutrient solution also contains a small amount of oxygen. It is added to the inner tank 1 through the water inlet pipe 3. The inner tank 1 acts as a buffer tank, where the oxygen in the newly added nutrient solution is first consumed by facultative anaerobic bacteria. When it reaches the water outlet 7 at the bottom, the remaining oxygen is completely consumed by the facultative anaerobic bacteria. At this point, the anaerobic nutrient solution then enters the outer tank 2 through the water outlet 7, ensuring that the nutrient solution in the outer tank 2 remains in an anaerobic state without environmental fluctuations. The anaerobic environment in the outer tank 2 is conducive to the large-scale reproduction of anaerobic ammonia-oxidizing bacteria. Therefore, this invention provides a stable and favorable growth and reproduction environment for anaerobic ammonia-oxidizing bacteria through the structure of the inner tank 1 and the outer tank 2.

[0025] The top of the outer tank 2 is also equipped with an overflow device. In order to ensure that the tank is always kept in a stable environment, the waste liquid that has been cultured with bacteria is pushed by the newly added nutrient pressure to the overflow device at the top of the outer tank 2 and flows out of the outer tank 2.

[0026] The bottom of the outer tank 2 is equipped with a discharge pipe 14. When the conditions reach the preset standard, anaerobic ammonia oxidizing bacteria are released through the discharge pipe 14 to complete the collection of anaerobic ammonia oxidizing bacteria. The top of the tank is sealed by the tank cover 17. The tank cover 17 is equipped with an exhaust pipe 18, which is connected to a water seal to prevent the entry of outside air and ensure a good anaerobic environment.

[0027] The overflow device described above is further refined to include two types of devices: an overflow weir 10 and an overflow pipe 15. The overflow weir 10 is located at the top of the outer tank 2. The initial height of the added nutrient solution is lower than the height of the overflow weir 10. When the anaerobic ammonia-oxidizing bacteria consume the nutrients in the nutrient solution, nutrient solution needs to be added to the inner tank 1 through the water inlet pipe 3. The newly added nutrient solution will squeeze the waste liquid in the tank. The waste liquid will flow into the outer tank 2 through the water hole 7. As the newly added nutrient solution continuously enters the inner tank 1, the waste liquid from the inner tank 1 to the outer tank 2 will also continuously approach the overflow weir 10 at the top of the outer tank 2. Finally, the waste liquid will flow out of the outer tank 2 through the overflow weir 10. Because the bacteria in the tank will settle on their own and the rate of adding new nutrient solution through the water inlet pipe 3 is controlled, the bacteria in the tank will not flow out of the outer tank 2 with the waste liquid. The overflow weir 10 is connected to the water outlet pipe 11. The waste liquid flows into the waste liquid collector through the water outlet pipe 11 to complete the collection of waste liquid.

[0028] The overflow pipe 15 is also located at the top of the outer tank 2, and above the overflow weir 10. The purpose of setting the overflow pipe 15 is to prevent damage to the fermenter as a whole due to excessive liquid intake caused by misoperation. If such a situation occurs, the excess fermentation liquid can be discharged from the outside of the tank through the overflow pipe 15 to avoid pressure shock to the fermenter and to protect the fermenter.

[0029] Based on the above, the overflow weir 10 is set in a sawtooth shape, which can form a stable water level difference, making it easier to observe and measure the flow rate of waste liquid. At the same time, it can effectively disperse the water flow, avoid eddies or disordered stratification, make the water flow more evenly distributed, reduce local scouring or sedimentation problems, and thus better ensure the stability of the tank environment.

[0030] Based on the above, a central pipe 4 is installed at the top of the inner tank 1. The water outlet of the inner tank is located at the upper end of the central pipe 4. The inoculation pipe 5 extends from the upper end of the central pipe 4 to the lower part of the central pipe 4. The position of the water outlet of the inner tank of the water inlet pipe 3 is higher than that of the water outlet of the inoculation pipe 5. When adding nutrient solution, the nutrient solution enters the inner tank 1 through the water inlet pipe 3. At the same time, because the position of the water outlet of the inner tank of the water inlet pipe 3 is higher than that of the water outlet of the inoculation pipe 5, the inoculum at the water outlet of the inoculation pipe 5 can be further flushed when the nutrient solution is added, ensuring that it is fully mixed into the nutrient solution. The lower end of the central pipe 4 is set as an inverted horn-shaped water intake structure, which further improves the efficiency and coverage of the inoculum and nutrient solution when injecting them, thereby ensuring that the inoculum is more evenly dispersed in various parts of the tank.

[0031] Based on the above, a composite monitoring instrument 9 is installed in the outer tank 2. The composite monitoring instrument 9 can measure the temperature (°C), pH and dissolved oxygen (DO) of the nutrient solution. The composite monitoring instrument 9 can monitor various data of the tank environment at any time to realize real-time monitoring of the tank environment.

[0032] The top of the inner tank 1 is also equipped with a drug inlet pipe 6. When the monitoring data in the composite monitor 9 does not meet the environmental standards, the corresponding drugs are added externally through the drug inlet pipe 6 in a timely manner to adjust the environment inside the tank and ensure that the environment inside the tank always maintains the set standards.

[0033] Based on the above, multiple sampling tubes 12 are vertically spaced on the outer wall of the outer tank 2. When the inoculum is added and the culture begins to run for a set time, colorimetric detection is performed based on the sampling results obtained from different sampling positions on the outer tank 2. The feedback from the colorimetric detection determines whether to continue the culture or open the discharge tube 14 to discharge the bacteria. The sampling tubes 12 are not necessary; the bacteria can be discharged directly according to the set time. The setting of the sampling tubes 12 further refines the sampling time, making the extracted anaerobic ammonia oxidizing bacteria more in line with the standards and of better quality.

[0034] Based on the above, valves 16 are provided at the external outlets of the inoculation tube 5, sampling tube 12 and discharge tube 14, and water seals are provided at the exhaust pipe 18, water outlet pipe 11 and overflow pipe 15. During the cultivation of anaerobic ammonia oxidizing bacteria, air is prevented from entering the tank, thereby disrupting the environment for cultivating anaerobic ammonia oxidation, and the stability of the cultivation environment inside the tank is better guaranteed.

[0035] Based on the above, a manhole 13 was added to the side wall of the outer tank 2. When the equipment inside the tank malfunctions and needs to be repaired or replaced, the manhole 13 can be opened to allow maintenance personnel to enter for repair.

[0036] The lid 17 is also provided with a lifting hole 21, which corresponds to the position where the submersible mixer 8 is placed. It is located directly above the position where the submersible mixer 8 is placed. Normally, the lifting hole 21 is sealed. The sealing method can be selected according to actual needs. When the submersible mixer 8 needs to be repaired or replaced, the submersible mixer 8 can be lifted out of the lifting hole 21 by the corresponding lifting device to achieve the purpose of repair and replacement.

[0037] The lifting device can be freely selected according to the actual situation, such as electric hoist 20, electric winch, lifting pulley, etc.

[0038] In addition, railings 19 are installed around the can lid 17 to further enhance safety during maintenance work.

[0039] The present invention also provides a method for culturing anaerobic ammonia-oxidizing bacteria, comprising the following steps: S1: A fixed amount of nutrient solution is fed into the inner tank 1 through the water inlet pipe 3; The composition and amount of nutrient solution can be freely selected according to actual needs. Taking high ammonia nitrogen wastewater as an example, the nutrient solution made with high ammonia nitrogen wastewater as the main component is mixed with activated carbon powder at a ratio of 1:10 to 1:3. After stirring evenly, it is added into the tank through the water inlet pipe 3 and the central pipe 4 until it reaches 2 / 3 to 4 / 5 of the tank's reaction volume. Because the structure of the inner tank 1 and the outer tank 2 is similar to that of communicating vessels, the inner tank 1 and the outer tank 2 are connected through the water passage 7. According to the principle of communicating vessels, the nutrient solution in the inner tank 1 and the outer tank 2 are at the same liquid level at this time.

[0040] S2: A certain amount of facultative anaerobic bacteria is introduced into the inner tank 1 through the inoculation tube 5, and after a set time; The nutrient solution fed into inner tank 1 contains trace amounts of oxygen. Anaerobic ammonia oxidizing bacteria require strictly anaerobic conditions to grow. On one hand, inner tank 1 acts as a buffer tank, providing an anaerobic environment for outer tank 2. Facultative anaerobic bacteria inside the tank are responsible for consuming the oxygen in the nutrient solution. When the nutrient solution from inner tank 1 enters outer tank 2 through the water passage 7 at the bottom of inner tank 1, the nutrient solution environment is basically in an anaerobic state. After adding facultative anaerobic bacteria, they will also enter outer tank 2 through the water passage 7. Although the nutrient solution initially added to outer tank 2 contains trace amounts of oxygen, the added facultative anaerobic bacteria will also enter outer tank 2 through the water passage 7. In an aerobic environment, facultative anaerobic bacteria become the dominant species and will multiply rapidly, consuming the oxygen in both inner tank 1 and outer tank 2. After a period of time, the nutrient solution in outer tank 2 will also become anaerobic. In an anaerobic environment, the growth of facultative anaerobic bacteria will be inhibited, making anaerobic ammonia oxidizing bacteria the dominant species and creating the prerequisites for providing a suitable environment for anaerobic ammonia oxidizing bacteria.

[0041] On the other hand, the inner tank 1 serves as an adaptation tank. A mixture of facultative anaerobic bacteria and anaerobic ammonia oxidizing bacteria is inoculated into the inner tank 1 through the inoculation tube 5. At this time, the facultative anaerobic bacteria start to grow on the surface of the activated carbon, consuming a small amount of organic matter in the nutrient solution and oxidizing ammonia nitrogen into nitrite nitrogen in a low-oxygen environment, providing nitrite for the subsequent anaerobic ammonia oxidizing bacteria. The anaerobic ammonia oxidizing bacteria then lie dormant in the oxygen-deficient zone inside the activated carbon, in a stagnant buffer period.

[0042] S3: A certain amount of anaerobic ammonia-oxidizing bacteria is introduced into the inner tank 1 through the inoculation tube 5, and after a set time.

[0043] After step S2 is completed, the environment inside the tank is suitable for the growth of anaerobic ammonia oxidizing bacteria. At this time, a large number of anaerobic ammonia oxidizing bacteria are added. As the dominant bacteria, anaerobic ammonia oxidizing bacteria will grow and reproduce in large quantities in inner tank 1 and outer tank 2. After a specific period of time, the cultivation of anaerobic ammonia oxidizing bacteria is completed.

[0044] S4: Discharge bacteria through the discharge tube 14.

[0045] After the set time has elapsed, the anaerobic ammonia oxidizing bacteria have completed their cultivation and multiplied in large quantities. At this point, the bacteria are discharged through the discharge tube 14, thus completing the cultivation of the anaerobic ammonia oxidizing bacteria.

[0046] Based on the above, in order to further improve the quality of the collected anaerobic ammonia oxidizing bacteria, activated carbon powder can be added as needed when adding the nutrient solution in step S1. Activated carbon can provide an anaerobic environment for the anaerobic ammonia oxidizing bacteria, adsorb the bacteria, and promote the rapid attainment of a anaerobic ammonia oxidizing bacteria concentration of 10. 6 The clumping conditions of cells / mL facilitate the formation of granular sludge that settles easily, effectively preventing bacterial loss. It also absorbs microbial metabolites, removes impurities from the tank, and helps maintain the stability of the tank environment.

[0047] Based on the above, in order to further improve the quality of the collected anaerobic ammonia oxidizing bacteria, after completing step S1, the submersible mixer 8 is started to allow the nutrient solution in the tank to flow. Subsequently, facultative anaerobic bacteria are added and mixed evenly with the nutrient solution, ensuring that the oxygen in the nutrient solution is fully consumed by the facultative anaerobic bacteria. The timing and duration of starting the submersible mixer 8 can be freely selected according to changes in the tank environment. Alternatively, the submersible mixer 8 can be started after step S2 is completed. At this time, after adding the anaerobic ammonia oxidizing bacteria, the anaerobic ammonia oxidizing bacteria and the nutrient solution can fully react, improving the utilization rate of the nutrient solution and increasing the cultivation efficiency of the anaerobic ammonia oxidizing bacteria.

[0048] Based on the above, in order to further clarify the time required for the cultivation of anaerobic ammonia-oxidizing bacteria, sampling tubes 12 at different locations are set on the outer wall of the outer tank 2. The number and location of the sampling tubes 12 can be freely selected according to actual needs. The samples taken from the sampling tubes 12 are subjected to colorimetric detection. If the samples meet the cultivation standards according to the set standards, the discharge tube 14 is opened to discharge bacteria. If the samples do not meet the set standards, fermentation continues until the sampling standards are met. At the same time, based on the experimental results of the samples taken from the sampling tubes 12, the corresponding medicines are added to the inner tank 1 through the dosing tube 6 to adjust the environment. The choice of medicines can be based on actual needs, such as nitrate nitrogen or nitrite nitrogen.

[0049] Based on the above, the present invention also supports access to a remote control system. The composite monitor 9 automatically monitors parameters such as pH, temperature (°C), and oxygen content (DO) in the tank. It is connected to the remote control platform and transmits the corresponding data to the remote control platform in real time, realizing remote intelligent monitoring and operation.

[0050] Based on the above, in order to facilitate the maintenance of the submersible mixer 8, an electric hoist 20 corresponding to the submersible mixer 8 is installed on the inner wall of the tank. When it is necessary to maintain the submersible mixer 8, the electric hoist 20 is used to lift the submersible mixer 8 and transport it out of the tank through the lifting hole 21 for equipment maintenance.

[0051] The following is a specific embodiment that further illustrates the anaerobic ammonia-oxidizing bacteria cultivation method provided by the present invention: Nutrient solution made primarily from high ammonia nitrogen wastewater is mixed with activated carbon powder at a ratio of 1:10 to 1:3. The specific ratio can be freely selected according to actual needs. After stirring evenly, the solution is added into the tank through inlet pipe 3 and guided through central pipe 4, filling the tank to 2 / 3 to 4 / 5 of the reaction volume. The amount of nutrient solution added can be selected according to the number of anaerobic ammonia oxidizing bacteria to be cultivated and other specific circumstances.

[0052] Because the inner tank 1 and the outer tank 2 are connected by the water passage 7, according to the principle of communicating vessels, the nutrient solution in the inner tank 1 and the outer tank 2 are at the same level. Then, the mixed sludge of facultative anaerobic bacteria and anaerobic ammonia oxidizing bacteria is introduced through the inoculation pipe 5. The facultative anaerobic bacteria are screened by the primary denitrification tank of the landfill leachate and diluted into liquid for later use. The anaerobic ammonia oxidizing bacteria are acclimated and screened by the landfill leachate and diluted into liquid for later use. The source of the facultative anaerobic bacteria and anaerobic ammonia oxidizing bacteria can be selected according to the actual situation. This is only an example of a source of facultative anaerobic bacteria.

[0053] At startup, first introduce facultative anaerobic bacteria into the tank. After reacting for 2 to 10 hours, introduce anaerobic ammonia oxidizing bacteria. The specific reaction time can be freely adjusted according to the tank environment and the oxygen content (DO) data displayed on the composite monitoring instrument 9. The inoculation amount of facultative anaerobic bacteria is about 1% to 10% of the inoculation amount of anaerobic ammonia oxidizing bacteria, mainly to consume the trace oxygen contained in the nutrient solution during injection. The inoculation amount of anaerobic ammonia oxidizing bacteria accounts for 6% to 20% of the total tank volume. The inoculation amounts of facultative anaerobic bacteria and anaerobic ammonia oxidizing bacteria can be freely selected according to actual needs and the tank environment. Then, fill the tank with nutrient solution containing activated carbon powder as needed. At this time, turn on the submersible mixer 8 and react for 25 to 30 days. The start time and timing of the mixer 8 can be freely selected according to specific circumstances. The reaction time can also be selected according to actual needs. During the reaction, samples of bacteria in the tank can be taken for testing. The reaction can be continued based on the test results. Then, the concentration of anaerobic ammonia oxidizing bacteria in the tank is detected through the sampling tube 12. When the sample taken from the sampling tube 12 in the middle of the tank has a red-green axis a value greater than 20 through a colorimeter, the discharge tube valve is opened to discharge bacteria. The discharge is stopped when the a value of the sample taken from the sampling tube 12 at the lowest point of the tank is around 30. Otherwise, fermentation continues until the conditions are met. The detection method here is only an example. Specific detection methods and standards can be formulated according to actual needs.

[0054] The fermentation process is monitored by a composite monitoring instrument 9, which monitors the pH, temperature (°C), and dissolved oxygen (DO) inside the tank. The pH inside the tank is maintained at 6.8~8.0, the temperature is controlled at 28℃-38℃, DO < 0.1 mg / L, the COD of the nutrient solution is controlled below 800 mg / L, the ammonia nitrogen concentration is controlled at 50 mg / L~450 mg / L, and the nitrite nitrogen concentration is controlled at 50 mg / L~300 mg / L. Depending on actual needs, nitrate nitrogen or nitrite nitrogen can be added externally through the dosing pipe 6, with the molar ratio controlled within the range of 1:1.1~1.35. The nutrient solution is replenished when the bacteria are discharged using the discharge pipe 14. The reaction temperature is adjusted and controlled by the temperature of the added nutrient solution. The above data are just examples, and the specific values ​​can be freely adjusted according to actual needs. The above parameters are transmitted to the remote control system through the composite monitoring instrument 9 for remote control.

[0055] If the equipment malfunctions, it can be inspected by opening the manhole 13 and entering the tank. If the submersible mixer 8 malfunctions, it can be lifted by the electric hoist 20 installed on the inner wall of the tank and transported out of the tank through the lifting hole 21, thereby enabling the submersible mixer 8 to be repaired or replaced.

[0056] Specific examples have been used to illustrate the principles and implementation methods of this invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this invention. Furthermore, those skilled in the art will recognize that, based on the ideas of this invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of this invention.

Claims

1. An anaerobic ammonia-oxidizing bacteria cultivation device, characterized in that, The application relates to an anaerobic ammonia oxidation bacteria culture device. The device comprises an outer tank (2) and an inner tank (1) arranged in the outer tank (2), the inner tank (1) is provided with a water inlet pipe (3) for injecting nutrient solution and a bacteria inoculation pipe (5) for injecting bacteria, the inner tank (1) is communicated with the outer tank (2) through a water passing hole (7) arranged at the bottom of the inner tank (1), the top of the outer tank (2) is provided with an overflow device, the bottom of the outer tank (2) is provided with a bacteria discharging pipe (14), the height of the overflow device is lower than that of the water inlet pipe (5), and the top of the tank body is provided with a tank cover (17).

2. The anammox bacteria culturing apparatus according to claim 1, wherein The overflow device comprises an overflow weir (10) for discharging waste liquid and an overflow pipe (15), the overflow weir (10) is connected with a water outlet pipe (11), and the overflow pipe (15) is arranged at a height higher than that of the overflow weir (10).

3. The culture apparatus for anaerobic ammonia-oxidizing bacteria according to claim 2, wherein The overflow weir (10) is sawtooth-shaped.

4. The culture apparatus for anaerobic ammonia-oxidizing bacteria according to claim 1, wherein The top of the inner tank is further provided with a central pipe (4) for guiding flow, the water outlet of the water inlet pipe (3) is arranged on the upper end of the central pipe (4), the bacteria inoculation pipe (5) extends to the middle and lower parts of the central pipe (4) through the upper end of the central pipe (4), and the lower end of the central pipe (4) is a water suction horn structure.

5. The culture apparatus for anaerobic ammonia-oxidizing bacteria according to claim 1, wherein The outer tank (2) is further provided with a composite monitor (9) for measuring the temperature, pH value and dissolved oxygen content of the nutrient solution, and the top of the inner tank (1) is further provided with a medicine adding pipe (6) for adding medicine.

6. The culture apparatus according to any one of claims 1 to 5, wherein A plurality of sampling pipes (12) for sampling are vertically and spacedly arranged on the outer wall of the outer tank (2).

7. The culture apparatus according to any one of claims 6, wherein Valves (16) are arranged at the tank external outlets of the bacteria discharging pipe (14), the bacteria inoculation pipe (5) and the sampling pipe (12).

8. The culture apparatus according to any one of claims 1 to 5, wherein The bottoms of the inner tank (1) and the outer tank (2) are provided with submersible mixers (8) for promoting the mixing of the nutrient solution and the bacteria.

9. The anammox bacteria culturing apparatus according to claim 8, wherein A lifting hole (21) is formed in the tank cover (17), and the lifting hole (21) serves as a channel for taking out or putting in the submersible mixer (8) when the submersible mixer (8) needs to be replaced or repaired.

10. A method for culturing anammox bacteria, characterized by, The application further discloses an application method of the anaerobic ammonia oxidation bacteria culture device. S1: a certain amount of nutrient solution is input into the inner tank (1) through the water inlet pipe (3); S2: a certain amount of facultative bacteria is input into the inner tank (1) through the bacteria inoculation pipe (5) and is kept for a certain time; S3: a certain amount of facultative bacteria is input into the inner tank (1) through the bacteria inoculation pipe (5) and is kept for a certain time; S4: bacteria are discharged through the bacteria discharging pipe (14).

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