Buffering and transmission device for compressing width of chromatographic common band and use method of buffering and transmission device

Abstract

The invention provides a buffering and transmission device for compressing the width of a chromatographic common band and a use method of the buffering and transmission device. The device comprises a buffer area and a transmission area, the buffer area is provided with a storage chamber, and the storage chamber is provided with an inlet and an outlet; the transmission area comprises a bracket as well as a first transmission mechanism and a second transmission mechanism which are mounted on the bracket, and the first transmission mechanism comprises a lower guide pipe fixed on the bracket and is used for conveying the chromatographic column into the storage chamber through the lower storage box, the lower guide pipe and the inlet; and the second transmission mechanism comprises an upper guide pipe fixed on the bracket and is used for conveying the chromatographic column from the storage chamber to the upper storage box through the outlet and the upper guide pipe. The sensitivity of the chromatographic instrument can be improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of gas chromatography analysis, in particular, especially relates to a buffer and transmission device for chromatographic universal bandwidth compression and a use method thereof. BACKGROUND

[0002] The sensitivity of gas chromatography is crucial in the parameters of analytical instruments, if you want to improve the sensitivity of chromatography, the usual method is to increase the concentration of the sample, or use more advanced detectors, etc., in addition to these conventional ways, there are other methods, but not too ideal, such as end cooling will cause the chromatographic peak width increases; Column head pressure column flow rate changes too much, not only will cause baseline fluctuation but also will cause the column efficiency is reduced seriously; Column tail pressure must be effective in the period when the component band has passed the pressure junction point and has not reached the detector, and it is impossible to do both ends for slightly complex samples. To realize the real meaningful gas chromatography band compression, it is necessary to ensure that the separation degree of complex components does not decrease significantly during the compression process. The existing work in the field of gas chromatography at home and abroad has not achieved this point. The present application provides a device for improving the sensitivity of chromatography, and the actual sample verification results show that the device can effectively compress the peak width by more than 10 times under the premise of maintaining 80% separation degree, thereby improving the signal-to-noise ratio and sensitivity by 10 times, which is a relatively ideal device. SUMMARY

[0003] According to the above technical problems, a buffer and transmission device for chromatographic universal bandwidth compression and a use method thereof are provided.

[0004] The technical means adopted by the present application are as follows:

[0005] A buffer and transmission device for chromatographic universal bandwidth compression, comprising: a buffer area and a transmission area, the buffer area is provided with a storage chamber, the storage chamber has an inlet and an outlet; the transmission area includes a support and a first transmission mechanism and a second transmission mechanism mounted on the support, the first transmission mechanism includes a lower guide pipe fixed on the support, for transmitting the chromatographic column into the storage chamber through the lower storage box, the lower guide pipe and the inlet; the second transmission mechanism includes an upper guide pipe fixed on the support, for transmitting the chromatographic column from the storage chamber to the upper storage box through the outlet and the upper guide pipe.

[0006] Further, the buffer area includes a cooling plate and a transparent cover plate, the front surface of the cooling plate is provided with a groove, the inlet and the outlet are communicated with the groove, the transparent cover plate covers the front surface of the cooling plate, and the storage chamber is formed between the groove and the transparent cover plate; the back surface of the cooling plate is a cold area, which has a cooling function.

[0007] Further, the buffer zone further comprises a guide block fixed to a corner of the lower end of the cooling plate, opposite the lower chromatographic column inlet, for guiding the chromatographic column entering the buffer zone upwards.

[0008] Further, the buffer zone further comprises a plurality of drag reduction blocks distributed on both sides of the groove of the cooling plate, and the chromatographic column entering the storage chamber is moved on the drag reduction blocks.

[0009] Further, the first transmission mechanism further comprises a lower driving wheel, a lower driven wheel, a lower motor belt and a lower motor, the lower conduit is fixed to the middle part close to the contact line of the lower driving wheel and the lower driven wheel, the lower driving wheel is in contact with the lower driven wheel, the chromatographic column passes between the lower driving wheel and the lower driven wheel, and the chromatographic column is driven by the friction force between the lower driving wheel and the lower driven wheel through the rolling of the lower driving wheel.

[0010] The output shaft of the lower motor is connected to one side of the lower motor belt, the other side of the lower motor belt is connected to the lower driving wheel shaft, the lower driving wheel is installed on the lower driving wheel shaft, the lower motor belt drives the lower driving wheel shaft to rotate through the lower motor, and the lower driving wheel is driven to rotate.

[0011] Further, the lower driven wheel is installed on the lower driven wheel shaft, and lower pre-tightening columns are threadedly connected to both ends of the lower driven wheel shaft, and the distance between the lower driven wheel and the lower driving wheel is adjusted through threads.

[0012] Further, the second transmission mechanism comprises an upper driving wheel, an upper driven wheel, an upper motor belt and an upper motor, the upper conduit is fixed to the middle part close to the contact line of the upper driving wheel and the upper driven wheel, the upper driving wheel is in contact with the upper driven wheel, the chromatographic column passes between the upper driving wheel and the upper driven wheel, and the chromatographic column is driven by the friction force between the upper driving wheel and the upper driven wheel through the rolling of the upper driving wheel.

[0013] The output shaft of the upper motor is connected to one side of the upper motor belt, the other side of the upper motor belt is connected to the upper driving wheel shaft, the upper driving wheel is installed on the upper driving wheel shaft, the upper motor belt drives the upper driving wheel shaft to rotate through the upper motor, and the upper driving wheel is driven to rotate.

[0014] Further, the upper driven wheel is installed on the upper driven wheel shaft, and upper pre-tightening columns are threadedly connected to both ends of the upper driven wheel shaft, and the distance between the upper driven wheel and the upper driving wheel is adjusted through threads.

[0015] The application also provides a use method of the buffer and transmission device for chromatographic strip width compression, comprising the following steps:

[0016] Step 1, cool the cooling plate of the buffer zone to the required temperature, and divide a part of the main chromatographic column, sequentially pass through the lower storage box, the lower conduit, the buffer zone, the upper conduit, the upper storage box, and finally reach the detector, at this time, the lower storage box stores the maximum amount of chromatographic column, and the buffer zone and the upper storage box are in the minimum state;

[0017] Step 2, raise the temperature in the column oven to a certain temperature, at this time, the lower driving wheel of the transmission area drives the lower driven wheel to rotate at a set speed, slowly guide the chromatographic column in the lower storage box into the buffer zone, fill the buffer zone with chromatographic columns around, and stop the rotation of the lower driving wheel at the same time of filling, and the guiding stops;

[0018] Step 3, raise the temperature in the column oven to a higher temperature, at this time, the upper driving wheel of the transmission area drives the upper driven wheel to rotate at a set speed, quickly guide the chromatographic column in the buffer zone into the upper storage box, when the chromatographic column in the buffer zone is the minimum amount and the upper storage box is the maximum amount, the upper driving wheel stops rotating, and the guiding stops; thus, the overall process is completed.

[0019] Compared with the prior art, the present application has the following advantages:

[0020] 1. The present application is suitable for chromatographic bandwidth compression technology, and the sensitivity of the chromatographic instrument can be improved through the device.

[0021] 2. The buffer zone provided in the present application can temporarily store chromatographic columns, and the transmission area can transmit the chromatographic columns in and out.

[0022] 3. The transparent cover plate is arranged on the cooling plate, so that the chromatographic column cannot fall out. The back of the cooling plate is a cold area, and the cooling and cooling can be realized by water cooling or semiconductor refrigeration and other cooling means. The guide block is arranged to guide the chromatographic column entering the buffer zone upwards, so as to avoid the chromatographic column from being broken at one corner. The friction-reducing block is arranged to make the chromatographic column move on the friction-reducing block, so as to reduce the friction of point contact, and the chromatographic column can be more easily guided in and out.

[0023] 4. The upper driving wheel drives the upper driven wheel to roll and drive the chromatographic column to transmit by friction, and the lower driving wheel drives the lower driven wheel to roll and drive the chromatographic column to transmit by friction. The upper and lower pre-tightening columns are arranged to adjust the distance between the driven wheels and the corresponding driving wheels through threads, so that the chromatographic column is not pressed too tightly to damage the chromatographic column, or pressed too loosely to slip.

[0024] Based on the above reasons, the present application can be widely popularized in the field of gas chromatographic analysis. BRIEF DESCRIPTION OF DRAWINGS

[0025] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or prior art description. Obviously, the drawings described in the following are only some embodiments of the present application, and the ordinary skilled in the art can obtain other drawings according to these drawings without any creative effort.

[0026] Fig. 1 Figure 1 is a schematic diagram of the main structure of a buffer and transmission device for chromatographic bandwidth compression according to the present application.

[0027] Fig. 2 Figure 2 is a schematic diagram of a partial structure according to the present application.

[0028] Figure 1: 1, buffer zone; 2, transmission zone;

[0029] 1-1, cooling plate; 1-2, guide block; 1-3, drag-reducing block; 1-4, transparent cover plate;

[0030] 2-1, upper guide pipe; 2-2, lower guide pipe; 2-3, upper driving wheel; 2-4, lower driving wheel; 2-5, upper driven wheel; 2-6, lower driven wheel; 2-7, upper motor belt; 2-8, lower motor belt; 2-9, upper motor; 2-10, lower motor; 2-11, upper pre-tightening column; 2-12, lower pre-tightening column. DETAILED DESCRIPTION

[0031] It should be noted that the embodiments and features in the present application can be combined with each other without conflict. The present application will be described in detail below with reference to the drawings and in combination with the embodiments.

[0032] In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below in combination with the drawings of the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, but not all the embodiments. The description of the at least one exemplary embodiment is actually only illustrative, but not intended to limit the present application and its application or use in any way. Based on the embodiments in the present application, all other embodiments obtained by the ordinary skilled in the art without any creative effort are within the scope of protection of the present application.

[0033] It should be noted that the terms used herein are only for describing specific embodiments, and are not intended to limit the exemplary embodiments according to the present application. As used herein, the singular form is intended to include the plural form, unless the context clearly indicates otherwise, and it should also be understood that when the terms "comprise" and / or "include" are used in the specification, there is a feature, step, operation, device, component and / or combination thereof.

[0034] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps described in these embodiments do not limit the scope of the invention. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following figures denote similar items; therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0035] In the description of this invention, it should be understood that the orientation or positional relationship indicated by directional terms such as "front, back, up, down, left, right", "horizontal, vertical, horizontal" and "top, bottom" is generally based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing this invention and simplifying the description. Unless otherwise stated, these directional terms do not indicate or imply that the device or element referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation on the scope of protection of this invention. The directional terms "inner" and "outer" refer to the inner and outer contours relative to the outline of each component itself.

[0036] For ease of description, spatial relative terms such as "above," "over," "on the upper surface of," "above," etc., are used herein to describe the spatial positional relationship of a device or feature as shown in the figures to other devices or features. It should be understood that spatial relative terms are intended to encompass different orientations in use or operation besides the orientation of the device as described in the figures. For example, if the device in the figures is inverted, a device described as "above" or "above" other devices or structures would subsequently be positioned as "below" or "under" other devices or structures. Thus, the exemplary term "above" can include both "above" and "below." The device may also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatial relative descriptions used herein will be interpreted accordingly.

[0037] Furthermore, it should be noted that the use of terms such as "first" and "second" to define components is merely for the purpose of distinguishing the corresponding components. Unless otherwise stated, the above terms have no special meaning and therefore should not be construed as limiting the scope of protection of this invention.

[0038] Example 1

[0039] like Figs. 1-2 As shown, the present invention provides a buffer and transmission device for chromatographic band width compression, which mainly relates to chromatographic band width compression technology. It is a device suitable for compressing the band width and can improve the sensitivity of gas chromatography.

[0040] This invention discloses a buffer and transmission device for compressing chromatographic band width, mainly composed of a buffer zone 1 and a transmission zone 2. The buffer zone 1 (buffer zone) mainly includes a cooling plate 1-1, a guide block 1-2, several drag-reducing blocks 1-3, and a transparent cover plate 1-4. The transmission zone 2 mainly includes a support, an upper guide tube 2-1, a lower guide tube 2-2, an upper drive wheel 2-3, a lower drive wheel 2-4, an upper driven wheel 2-5, a lower driven wheel 2-6, an upper motor belt 2-7, a lower motor belt 2-8, an upper motor 2-9, a lower motor 2-10, an upper pre-tightening column 2-11, a lower pre-tightening column 2-12, an upper drive shaft, an upper driven shaft, a lower drive shaft, and a lower driven shaft. The upper guide tube 2-1, lower guide tube 2-2, upper motor 2-9, lower motor 2-10, upper drive shaft, upper driven shaft, lower drive shaft, and lower driven shaft are all mounted on the support. This device is suitable for chromatographic band width compression technology, and can improve the sensitivity of chromatographic instruments.

[0041] In a preferred embodiment, buffer zone 1 is a temporary storage area for the chromatographic column. Buffer zone 1 has a storage chamber with an inlet and an outlet. The front surface of cooling plate 1-1 has a groove for storing the chromatographic column, with both the inlet and outlet communicating with the groove. A transparent cover plate 1-4 covers cooling plate 1-1 to prevent the chromatographic column from detaching. The storage chamber is formed between the groove and the transparent cover plate 1-4. The back of cooling plate 1-1 is a cold zone with cooling and temperature reduction functions, which can be achieved through water cooling or other cooling methods such as semiconductor refrigeration. A guide block 1-2 is fixed to a corner of the lower end of cooling plate 1-1, located directly opposite the lower chromatographic column inlet, guiding the chromatographic column entering buffer zone 1 upwards to prevent breakage due to concentrated pressure at one corner. Several drag-reducing blocks 1-3 are distributed on both sides of the groove on the front surface of cooling plate 1-1. The chromatographic column moves against the drag-reducing blocks 1-3, with point contact reducing friction and facilitating column entry and exit.

[0042] In a preferred embodiment, transmission zone 2 is responsible for transmitting the chromatographic column in and out; wherein the upper guide tube 2-1 is fixed at the midpoint near the contact line between the upper drive wheel 2-3 and the upper driven wheel 2-5, and the lower guide tube 2-2 is fixed at the midpoint near the contact line between the lower drive wheel 2-4 and the lower driven wheel 2-6; the upper drive wheel 2-3 is installed in contact with the upper driven wheel 2-5, and the chromatographic column passes through the space between the upper drive wheel 2-3 and the upper driven wheel 2-5 and in the upper guide tube 2-1. The upper drive wheel 2-3 drives the upper driven wheel 2-5 to roll, thereby driving the chromatographic column by friction. The lower drive wheel 2-4 is installed in contact with the lower driven wheel 2-6, and the chromatographic column passes through the space between the lower drive wheel 2-4 and the lower driven wheel 2-6 and in the lower guide tube 2-2. The lower drive wheel 2-4 drives the lower driven wheel 2-6 to roll, thereby driving the chromatographic column by friction. The output shaft of the upper motor 2-9 is connected to one side of the upper motor belt 2-7. The other side of the upper motor belt 2-7 is connected to the upper drive wheel shaft. The upper drive wheel 2-3 is mounted on the upper drive wheel shaft. The upper motor 2-9 drives the upper motor belt 2-7, which in turn drives the upper drive wheel shaft, thus causing the upper drive wheel 2-3 to rotate. The output shaft of the lower motor 2-10 is connected to one side of the lower motor belt 2-8, and the other side of the lower motor belt 2-8 is connected to the lower drive wheel shaft. The lower drive wheel 2-4 is mounted on the lower drive wheel shaft. The lower motor 2-10 drives the lower motor belt 2-8, which in turn drives the lower drive wheel shaft, thus causing the lower drive wheel 2-4 to rotate. The upper and lower preload columns (upper preload column 2-11 and lower preload column 2-12) are installed at both ends of the corresponding driven wheel shafts. The distance between each driven wheel and the corresponding drive wheel can be adjusted by the thread, so that the chromatographic column is not pressed too tightly and damaged, nor is it pressed too loosely and slipped. Specifically: the upper driven wheel 2-5 is installed on the upper driven wheel shaft, and the two ends of the upper driven wheel shaft are threaded to the upper preload column 2-11. The distance between the upper driven wheel 2-5 and the upper driving wheel 2-3 is adjusted by the thread; the lower driven wheel 2-6 is installed on the lower driven wheel shaft, and the two ends of the lower driven wheel shaft are threaded to the lower preload column 2-12. The distance between the lower driven wheel 2-6 and the lower driving wheel 2-4 is adjusted by the thread.

[0043] Example 2

[0044] like Figs. 1-2 As shown, the present invention also provides a method for using a buffer and transmission device for compressing chromatographic band width, comprising the following steps:

[0045] Step 1: Cool the cooling plate 1-1 of buffer 1 to the required temperature, and separate a portion of the main column, passing it sequentially through the lower storage box, lower tubing 2-2, buffer 1, upper tubing 2-1, upper storage box, and finally to the detector. At this time, the lower storage box holds the maximum amount of column, while buffer 1 and the upper storage box hold the minimum amount.

[0046] Step 2: Raise the temperature inside the column oven to a certain level. At this time, the lower drive wheel 2-4 of the transmission zone 2 drives the lower driven wheel 2-6 to rotate. At the set speed, the chromatographic column in the lower storage box is slowly introduced into the buffer zone 1, filling the buffer zone 1 with the chromatographic column. When the buffer zone 1 is filled, the lower drive wheel 2-4 stops rotating, and the introduction stops.

[0047] Step 3: Raise the temperature inside the column oven to a higher level. At this time, the upper drive wheel 2-3 of the transmission zone 2 drives the upper driven wheel 2-5 to rotate, quickly introducing the chromatographic column in buffer zone 1 into the upper receiving box at the set speed. When the chromatographic column in buffer zone 1 is at its minimum volume and the upper receiving box is at its maximum volume, the upper drive wheel 2-3 stops rotating, and the introduction stops. This completes the overall process.

[0048] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A buffer and drive apparatus for chromatographic band width compression, characterized by, The application relates to a buffer area (1) and a transmission area (2), wherein the buffer area (1) is provided with a storage chamber having an inlet and an outlet; the transmission area (2) comprises a support and a first transmission mechanism and a second transmission mechanism mounted on the support; the first transmission mechanism comprises a lower guide pipe (2-2) fixed on the support and used for conveying a chromatographic column into the storage chamber through a lower storage box, the lower guide pipe (2-2) and the inlet; and the second transmission mechanism comprises an upper guide pipe (2-1) fixed on the support and used for conveying the chromatographic column from the storage chamber to an upper storage box through the outlet and the upper guide pipe (2-1). The buffer area (1) comprises a cooling plate (1-1) and a transparent cover plate (1-4); the front surface of the cooling plate (1-1) is provided with a groove, the inlet and the outlet are communicated with the groove, the transparent cover plate (1-4) covers the front surface of the cooling plate (1-1), and the storage chamber is formed between the groove and the transparent cover plate (1-4); and the back surface of the cooling plate (1-1) is a cold area and has a cooling function.

2. The buffer and drive apparatus for chromatographic strip bandwidth compression of claim 1, wherein, The buffer area (1) further comprises a guide block (1-2) fixed on one corner of the lower end of the cooling plate (1-1) and located opposite the lower chromatographic column inlet, and is used for guiding the chromatographic column entering the buffer area (1) upwards.

3. The buffer and drive apparatus for chromatographic strip bandwidth compression of claim 2, wherein, The buffer area (1) further comprises a plurality of resistance reduction blocks (1-3) distributed on the two side edges of the groove of the cooling plate (1-1), and the chromatographic column entering the storage chamber is moved by being placed on the resistance reduction blocks (1-3).

4. The buffer and drive apparatus for chromatographic strip bandwidth compression of claim 2, wherein, The first transmission mechanism further comprises a lower driving wheel (2-4), a lower driven wheel (2-6), a lower motor belt (2-8) and a lower motor (2-10); the lower guide pipe (2-2) is fixed on the middle part close to the contact line of the lower driving wheel (2-4) and the lower driven wheel (2-6); the lower driving wheel (2-4) is in contact with the lower driven wheel (2-6); the chromatographic column passes between the lower driving wheel (2-4) and the lower driven wheel (2-6); the lower driving wheel (2-4) drives the lower driven wheel (2-6) to roll and drive the chromatographic column to transmit by friction; 5. The buffer and drive apparatus for chromatographic strip bandwidth compression of claim 1, wherein, The output shaft of the lower motor (2-10) is connected with one side of the lower motor belt (2-8); the other side of the lower motor belt (2-8) is connected with a lower driving wheel shaft; the lower driving wheel (2-4) is mounted on the lower driving wheel shaft; the lower motor (2-10) drives the lower motor belt (2-8); the lower motor belt (2-8) drives the lower driving wheel shaft, so as to drive the lower driving wheel (2-4) to rotate. The lower driven wheel (2-6) is mounted on a lower driven wheel shaft; lower pre-tightening columns (2-12) are threadedly connected to the two ends of the lower driven wheel shaft; and the distance between the lower driven wheel (2-6) and the lower driving wheel (2-4) is adjusted by threads.

6. The buffer and drive apparatus for chromatographic strip bandwidth compression of claim 5, wherein, ​ 7. The buffer and drive apparatus for chromatographic strip bandwidth compression of claim 1, wherein, The second transmission mechanism comprises an upper driving wheel (2-3), an upper driven wheel (2-5), an upper motor belt (2-7) and an upper motor (2-9), the upper conduit (2-1) is fixed at the middle part close to the contact line of the upper driving wheel (2-3) and the upper driven wheel (2-5), the upper driving wheel (2-3) is in contact with the upper driven wheel (2-5), the chromatographic column passes between the upper driving wheel (2-3) and the upper driven wheel (2-5), and the upper driving wheel (2-3) drives the upper driven wheel (2-5) to roll to drive the chromatographic column to transmit by friction; The output shaft of the upper motor (2-9) is connected with one side of the upper motor belt (2-7), the other side of the upper motor belt (2-7) is connected with the upper driving wheel shaft, the upper driving wheel (2-3) is installed on the upper driving wheel shaft, the upper motor (2-9) drives the upper motor belt (2-7), the upper motor belt (2-7) drives the upper driving wheel shaft, so that the upper driving wheel (2-3) rotates.

8. The buffer and drive apparatus for chromatographic strip bandwidth compression of claim 7, wherein, The upper driven wheel (2-5) is installed on the upper driven wheel shaft, and the upper pre-tightening column (2-11) is threadedly connected to both ends of the upper driven wheel shaft, so that the distance between the upper driven wheel (2-5) and the upper driving wheel (2-3) is adjusted by threads.

9. A method of using a buffer and drive apparatus for chromatographic band width compression as claimed in any one of claims 1 to 8, characterised in that, The method comprises the following steps: Step 1, cool the cooling plate (1-1) of the buffer zone (1) to the required temperature, and divide a part of the main chromatographic column, and sequentially pass through the lower storage box, the lower conduit (2-2), the buffer zone (1), the upper conduit (2-1), the upper storage box, and finally reach the detector, at this time, the lower storage box stores the maximum amount of chromatographic column, and the buffer zone (1) and the upper storage box are in the minimum state; Step 2, raise the temperature in the column oven to a certain temperature, at this time, the lower driving wheel (2-4) of the transmission zone (2) drives the lower driven wheel (2-6) to rotate, and the chromatographic column in the lower storage box is introduced into the buffer zone (1) at a slow speed according to the set speed, the buffer zone (1) is filled with chromatographic columns around, and the lower driving wheel (2-4) stops rotating at the same time of filling, and the introduction stops; Step 3, raise the temperature in the column oven to a higher temperature, at this time, the upper driving wheel (2-3) of the transmission zone (2) drives the upper driven wheel (2-5) to rotate, and the chromatographic column in the buffer zone (1) is introduced into the upper storage box at a fast speed according to the set speed, when the chromatographic column in the buffer zone (1) is the minimum amount and the chromatographic column in the upper storage box is the maximum amount, the upper driving wheel (2-3) stops rotating, and the introduction stops; thus, the whole process is completed.

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