Anti-wrinkle firming oil composition containing water-soluble polypeptide as well as preparation method and application of anti-wrinkle firming oil composition

Abstract

The invention discloses an anti-wrinkle firming oil composition containing water-soluble polypeptide as well as a preparation method and application of the anti-wrinkle firming oil composition, and belongs to the technical field of cosmetics. The composition comprises the following components: vegetable fat, synthetic fat, skin feeling adjusting fat, an oil-soluble active component, an oil-soluble color mixing component, a surfactant, essence, a solvent and a water-soluble polypeptide active matter. Adding the water-soluble polypeptide active matter into a solvent, and dissolving to obtain an active matter solution; and mixing the vegetable fat, the synthetic fat, the oil-soluble active component and the skin feeling adjusting fat, adding the oil-soluble color adjusting component and the essence, stirring, adding the surfactant while stirring, and finally adding the active matter solution to obtain the anti-wrinkle firming composition. According to the invention, after the water-soluble active matter is wrapped by using a reverse micelle wrapping technology, the water-soluble active matter can be added into a pure oil system, and a pure oil product with good stability, skin permeability and anti-wrinkle and tightening effects is prepared.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an anti-wrinkle and firming oil composition containing water-soluble polypeptides, its preparation method, and its application. Background Technology

[0002] The use of oils for skincare in cosmetics is currently characterized by rapid market growth, high consumer demand, and upgrades in product efficacy and ingredients, moving towards higher-level effects. In the past, the mainstream effects of oil-based skincare focused on nourishing, moisturizing, and repairing. Now, with the advent of the era of functional skincare, there's a shift towards higher-level needs such as anti-aging, with anti-wrinkle and moisturizing becoming popular effects. Existing pure oil skincare products generally use basic oils (such as plant oils, animal oils, and synthetic oils), functional oils such as sea buckthorn fruit oil (antioxidant), shea butter (nourishing and repairing), and jojoba seed oil (regulating oil and water balance), vitamins such as vitamin E for antioxidants and retinyl esters for anti-aging, plus some auxiliary ingredients to optimize product stability and improve the user experience and sensory experience, as well as added antioxidants and fragrances. However, it's difficult to incorporate water-soluble active ingredients into pure oil systems. Therefore, anti-wrinkle active ingredients such as acetyl hexapeptide-8, acetyl tetrapeptide-2, arginine / lysine peptides, oligopeptide-1, and carnosine, which are water-soluble peptides, cannot be effectively added to pure oil cosmetic systems. Therefore, most of the uniform-looking facial oils on the market have the following drawbacks: ① There are few oil-soluble anti-wrinkle and firming active ingredients available on the market, and their effects are not significant. Most anti-wrinkle and firming ingredients are water-soluble; ② Water-soluble peptides with good anti-wrinkle and firming effects cannot be added to pure oil systems. If they are added, they will precipitate or separate; ③ Pure oil systems have low skin penetration. Summary of the Invention

[0003] To address the aforementioned problems in the prior art, this invention provides an anti-wrinkle and firming oil composition containing water-soluble polypeptides, its preparation method, and its application.

[0004] To achieve the above objectives, the present invention provides the following technical solution: This invention provides an anti-wrinkle and firming oil composition containing water-soluble polypeptides, comprising the following components by weight: 5-40 parts of vegetable oil, 5-65 parts of synthetic oil, 0.5-10 parts of skin-feel-adjusting oil, 0.05-5 parts of oil-soluble active ingredient, 0.05-5 parts of oil-soluble color-adjusting ingredient, 0.5-10 parts of surfactant, 0.05-0.5 parts of fragrance, 0.1-5 parts of solvent, and 0.005-0.5 parts of water-soluble polypeptide active ingredient.

[0005] As a preferred embodiment of the present invention, the plant oil includes one or more of squalane, meadowfoam seed oil, olive oil, and jojoba oil; the synthetic oil includes one or more of ethylhexyl palmitate, caprylic / capric triglyceride, isopropyl myristate, dioctyl carbonate, isononyl isononanoate, and synthetic squalane; and the skin-feel-adjusting oil includes one or more of tridecyl trimellitate, pentaerythritol tetraisostearate, and diisostearate malate.

[0006] Plant oils and synthetic oils are used together as emollients. Dioctyl carbonate, isononyl isononanoate, and synthetic squalane in synthetic oils are light oils.

[0007] As a preferred embodiment of the present invention, the oil-soluble active ingredient includes bisabolol and / or coenzyme Q10; the oil-soluble coloring ingredient includes sea buckthorn fruit oil and / or comfrey oil.

[0008] Oil-soluble active ingredients have anti-inflammatory and soothing effects, while oil-soluble color-correcting ingredients have a color-correcting effect.

[0009] As a preferred embodiment of the present invention, the surfactant includes sorbitol polyether-30 tetraoleate; the fragrance includes one or more of rose essential oil, jasmine essential oil and camellia essential oil.

[0010] Surfactants encapsulate water-soluble active ingredients to form reverse micelle structures, while fragrances are used for flavoring.

[0011] As a preferred embodiment of the present invention, the solvent includes one or more of water, rose hydrosol, centella asiatica hydrosol, and bifida ferment filtrate; the water-soluble polypeptide active ingredient includes one or more of acetyl tetrapeptide-2, acetyl hexapeptide-8, arginine / lysine polypeptide, oligopeptide-1, and carnosine.

[0012] Solvents are used to dissolve water-soluble active ingredients, while peptides are the active ingredients that have anti-wrinkle and firming effects.

[0013] The present invention also provides a method for preparing the anti-wrinkle and firming oil composition according to the above description, comprising the following steps: weighing each component, adding water-soluble polypeptide active ingredients to a solvent and dissolving them evenly until transparent to obtain an active ingredient solution; mixing plant oils, synthetic oils, oil-soluble active ingredients, and skin-feel-adjusting oils evenly, then adding oil-soluble coloring ingredients and fragrances, stirring and mixing evenly, adding surfactants while stirring, and stirring evenly; finally adding the active ingredient solution to form a reverse micelle encapsulation system to obtain the anti-wrinkle and firming oil composition.

[0014] The present invention also provides the application of the anti-wrinkle and firming oil composition described above in the preparation of cosmetics with anti-wrinkle and firming effects.

[0015] Compared with the prior art, the present invention has the following beneficial effects: (1) This invention uses reverse micelle encapsulation technology to encapsulate peptides, which can enable water-soluble active ingredients to be added to the pure oil system to obtain pure oil products with good anti-wrinkle and firming effects, thereby increasing the penetration effect of active ingredients and achieving efficient skin care with oil.

[0016] (2) The reverse micelles of this invention are supramolecular "water pool" structures formed by the self-assembly of water, oil, and surfactant, with peptides acting as the "water core." The reverse micelles exhibit many unique supramolecular aggregation behaviors, spontaneously forming a uniform "water-in-oil" aggregate. Compared with classic encapsulation structures such as liposomes, the reverse micelle structure has a smaller particle size, lower toxicity, and controllable encapsulation rate. It can achieve "water-in-oil," break through the lipid membrane structure, has higher skin permeability, and a longer residence time.

[0017] (3) The composition prepared by reverse micelle encapsulation technology in this invention is a uniform and transparent liquid with good stability. It will not separate into layers after long-term storage and has good skin penetration, thus achieving good anti-wrinkle and firming effects. Detailed Implementation

[0018] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention. It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the present invention.

[0019] Furthermore, regarding the numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included within this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0020] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0021] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be obvious to those skilled in the art. This specification and embodiments are merely exemplary.

[0022] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0023] In the following examples and comparative examples, "parts" refer to parts by weight, and the raw materials used are all commercially available conventional raw materials, without any special limitations. These will not be repeated below.

[0024] Example 1 Anti-wrinkle and firming oil compositions were prepared using the types and amounts of raw materials listed in Table 1. Table 1. Types and amounts of raw materials used in Example 1

[0025]

[0026] The preparation steps are as follows: Product preparation: 1. Mix phase A jojoba oil, ethylhexyl palmitate, dioctyl carbonate, bisabolol, and tridecyl trimellitate evenly. Add phase B sea buckthorn fruit oil and phase C camellia essential oil and stir until evenly mixed. Add phase D2 acetyl hexapeptide-8 and arginine / lysine peptide to deionized water to obtain the active ingredient solution. 2. Add D1 phase sorbitol polyether-30 tetraoleate while stirring, and then slowly add the active ingredient solution to obtain a homogeneous and transparent essence oil, namely an anti-wrinkle and firming oil composition.

[0027] Example 2 Anti-wrinkle and firming oil compositions were prepared using the types and amounts of raw materials listed in Table 2. Table 2. Types and amounts of raw materials used in Example 2

[0028]

[0029] The preparation steps are the same as in Example 1.

[0030] Example 3 Anti-wrinkle and firming oil composition was prepared using the types and amounts of raw materials listed in Table 3: Table 3. Types and amounts of raw materials used in Example 3

[0031]

[0032] The preparation steps are the same as in Example 1.

[0033] Comparative Example 1 Same as Example 1, except that the raw materials do not contain sorbitol polyether-30 tetraoleate.

[0034] The preparation steps are as follows: (1) Preparation of the active substance system: Dissolve D2 phase deionized water, acetyl hexapeptide-8, and arginine / lysine peptides until homogeneous and transparent to obtain an active ingredient solution.

[0035] (2) Product preparation: 1. Mix phase A jojoba oil, ethylhexyl palmitate, dioctyl carbonate, bisabolol, and tridecyl trimellitate evenly, then add phase B sea buckthorn fruit oil and phase C camellia essential oil and stir until well mixed. 2. Add the active ingredient solution prepared in step (1) under stirring to obtain the final product.

[0036] Comparative Example 2 Same as Example 2, except that the raw materials do not contain Centella Asiatica hydrosol and carnosine.

[0037] The preparation steps are as follows: 1. Mix the A phase meadowfoam seed oil, isopropyl myristate, isononyl isononanoate, coenzyme Q10, bisabolol, and diisostearyl malate evenly, then add the B phase comfrey oil and the C phase rose essential oil and stir until well mixed. 2. Add D1 phase sorbitol polyether-30 tetraoleate while stirring to obtain the final product.

[0038] Comparative Example 3 The types and amounts of raw materials are the same as in Example 1. The difference lies in the preparation steps. In this comparative example, after weighing each raw material, they are all added to a container and then stirred to mix the raw materials and obtain the final product.

[0039] Effect verification 1. Observation results of the appearance of each group of products after standing for 72 hours: Table 4 Product Appearance of Each Group

[0040]

[0041] 2. Penetration effect The test samples were the products of Example 2 and Comparative Example 2.

[0042] 2.1 Experimental Objectives and Principles The permeability of coenzyme Q10 liposomes was analyzed based on the Franz diffusion cell principle. A quantitative method was used to study the transdermal permeability. Using isolated pig skin as the research model, liquid chromatography was used to detect the content of coenzyme Q10 in the receiving fluid and pig skin. The permeation volume and permeability were calculated. The transdermal permeability of liposomes was analyzed based on the above experimental data.

[0043] 2.2 Test Indicators This method is an in vitro method. The collected receiving fluid and skin are analyzed at 24-hour intervals to obtain the cumulative permeation amount and permeability coefficient of coenzyme Q10.

[0044] 2.3 Test Methods Take a piece of intact, undamaged pigskin, remove the fine hairs on its surface with tweezers, and remove the subcutaneous fat with a scalpel; then wash it with phosphate-buffered saline (PBS), wrap it in aluminum foil, and refrigerate it at -20°C until use.

[0045] The treated skin was thawed in PBS at pH 7.4 for 30 min, then cut into squares with sides of approximately 2.5 cm and fixed between the supply and receiving cells of the Franzs diffusion cell. A certain amount of Tween 80 was added to the PBS solution at pH 7.4 to make its mass fraction 5%, and then added to the receiving cell. 0.5 mL of the sample was added to the supply cell, and then the Franz diffusion cell was placed in a transdermal diffusion apparatus. The experimental temperature was set to 37 °C, the stirring speed to 600 r / min, and the reaction time to 12 h.

[0046] After the reaction was completed, the pigskin was removed, and the residual sample on the surface of the pigskin was removed with defatted cotton. One layer of pigskin was pasted with 3M tape and then removed. The remaining skin after the 3M tape treatment was cut into pieces and collected in a centrifuge tube. 2 mL of the extraction solvent isopropanol was added, and the mixture was sonicated for 30 min. The mixture was then centrifuged at 5000 r / min for 10 min using a refrigerated centrifuge. The supernatant was collected, filtered through a 0.22 μm organic membrane, and the content of coenzyme Q10 was determined by HPLC, which represents the content of coenzyme Q10 in the epidermis and dermis.

[0047] 2.4 Experimental Results Table 5. Permeation effect of Example 2

[0048]

[0049] Table 6. Permeation effect of Comparative Example 2

[0050]

[0051] Results: Compared with Comparative Example 2, Example 2 showed better permeability, indicating that the reverse micelle encapsulation system prepared by sorbitol polyether-30 tetraoleate increased the permeability of the composition.

[0052] 3. Anti-wrinkle and firming effect test The test samples were the products of Example 1 and Comparative Example 1.

[0053] 3.1 Experimental Objectives and Principles Elastase has the ability to degrade various proteins such as collagen and elastin. Changes in the structure of elastin during skin aging are a major cause of wrinkles and sagging skin. Studies have shown that inhibiting elastase from breaking down elastin can have a firming and anti-wrinkle effect. Because it can be used to inhibit the activity of elastase in the body, it has applications in the cosmetics industry and other industries.

[0054] The efficacy of test samples in reducing wrinkles and firming the skin is determined by using the elastase inhibition rate. The inhibition rate increases with the concentration of the test sample solution; a higher inhibition rate indicates that the sample effectively inhibits elastase activity and slows down the degradation of elastin, thus reducing wrinkles and firming the skin.

[0055] 3.2 Test Indicators Experimental indicator: elastase inhibition rate.

[0056] Judgment criteria: The higher the percentage of elastase inhibition in the test sample, the stronger the anti-wrinkle and firming effect of the test sample.

[0057] 3.3 Test Methods 3.3.1 The test samples were the products of Example 1 and Comparative Example 1.

[0058] 3.3.2 Sample pretreatment: Prepare sample solutions of several concentrations → Add appropriate concentrations of lichen red elastin and borate buffer solution to sample solution tubes of several concentrations respectively → Incubate each tube in a 37℃ water bath with constant temperature shaking for 10 min → Add elastase solution to each tube → Measure absorbance at 495 nm → Calculate elastase inhibition rate.

[0059] 3.4 Test Results Table 7 Results of elastase inhibition rate in Example 1

[0060]

[0061] Table 8 Results of elastase inhibition rate in Comparative Example 1

[0062]

[0063] As shown in Tables 7 and 8, Example 1 and Comparative Example 1 both exhibit significant inhibitory effects on elastase, with the inhibitory effect increasing with increasing concentration. Under the same concentration conditions, Example 1 showed a higher elastase inhibition rate and stronger anti-wrinkle and firming effect than Comparative Example 1, indicating that reverse micelle encapsulation enhances the efficacy of the active ingredient. It was verified that Example 3, like Example 1, also showed a good inhibition rate against elastase.

[0064] The above description is merely a preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

Claims

1. A water-soluble polypeptide-based anti-wrinkle and firming oil composition, characterized in that, By weight, it comprises the following components: 5-40 parts vegetable oil, 5-65 parts synthetic oil, 0.5-10 parts skin-feeling oil, 0.05-5 parts oil-soluble active ingredients, 0.05-5 parts oil-soluble coloring ingredients, 0.5-10 parts surfactant, 0.05-0.5 parts fragrance, 0.1-5 parts solvent, and 0.005-0.5 parts water-soluble polypeptide active ingredients.

2. The anti-wrinkle and firming oil composition according to claim 1, characterized in that, The plant oils include one or more of squalane, meadowfoam seed oil, olive oil, and jojoba oil; the synthetic oils include one or more of ethylhexyl palmitate, dioctyl carbonate, caprylic / capric triglyceride, isopropyl myristate, isononyl isononanoate, and synthetic squalane; the skin-feel-modifying oils include one or more of tridecyl trimellitate, pentaerythritol tetraisostearate, and diisostearate malate.

3. The anti-wrinkle and firming oil composition according to claim 1, characterized in that, The oil-soluble active ingredient includes bisabolol and / or coenzyme Q10; the oil-soluble coloring ingredient includes sea buckthorn fruit oil and / or comfrey oil.

4. The anti-wrinkle and firming oil composition according to claim 1, characterized in that, The surfactant includes sorbitol polyether-30 tetraoleate; the fragrance includes one or more of rose essential oil, jasmine essential oil and camellia essential oil.

5. The anti-wrinkle and firming oil composition according to claim 1, characterized in that, The solvent includes one or more of water, rose hydrosol, centella asiatica hydrosol, and bifida ferment filtrate; the water-soluble polypeptide active ingredient includes one or more of acetyl tetrapeptide-2, acetyl hexapeptide-8, arginine / lysine polypeptide, oligopeptide-1, and carnosine.

6. A method for preparing an anti-wrinkle and firming oil composition according to any one of claims 1 to 5, characterized in that, The process includes the following steps: weighing each component, adding the water-soluble polypeptide active ingredient to a solvent and dissolving it to obtain an active ingredient solution; mixing the plant oil, synthetic oil, oil-soluble active ingredient, and skin-feel-adjusting oil, then adding the oil-soluble color-adjusting ingredient and fragrance, stirring, then adding the surfactant while stirring, and finally adding the active ingredient solution to obtain the anti-wrinkle and firming oil composition.

7. The use of an anti-wrinkle and firming oil composition according to any one of claims 1 to 5 in the preparation of cosmetics with anti-wrinkle and firming effects.

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