Protein separation device

Abstract

The invention is applicable to the technical field of biological separation, and provides a protein separation device which comprises a base plate, the separation tank is arranged on the base plate; the tank cover is arranged on the separation tank and can be opened and closed; the hammering and tamping mechanism is assembled on the tank cover and is used for hammering and tamping materials in the separation tank; the driving mechanism is used for driving the hammering and tamping mechanism to operate; and the filtering mechanism is arranged on the base plate. According to the protein separation device provided by the scheme, through cooperative operation of all the mechanisms, the manual operation intensity is reduced, and the protein separation efficiency and uniformity are improved.

Description

Technical Field

[0001] This invention belongs to the field of bioseparation technology, and particularly relates to a protein separation device. Background Technology

[0002] Protein isolation is a key step in fields such as bioengineering, food processing, and pharmaceutical research and development. Its core requirement is to rapidly and efficiently isolate target proteins from biological raw materials (such as animal and plant tissues, microbial cells, etc.) while preserving the protein's activity as much as possible.

[0003] Currently, various technical solutions have been developed for different application scenarios in protein separation technology, but all of them have significant drawbacks: centrifugation equipment is expensive, has a complex operation process, and requires professional technicians for maintenance, making it only suitable for large-scale industrial production and difficult to adapt to the small-scale, multi-batch processing needs of laboratories; ultrasonic disruption technology, although having a high cell wall disruption efficiency, is prone to generating local high temperatures that can lead to protein denaturation and inactivation, and its high equipment maintenance costs and energy consumption limit its application in scenarios with stringent activity requirements; traditional manual pounding, as a common method for cell wall disruption, is not only labor-intensive and inefficient, but also difficult to precisely control the pounding force and frequency, resulting in uneven cell wall disruption, with some cells not being completely disrupted, affecting the protein release rate, while excessive pounding in some areas causes protein structure damage. In addition, manual operation lacks systematic impurity pretreatment and temperature control design, resulting in serious impurity interference in subsequent separation processes, making it difficult to meet the requirements of precise experiments for stable separation results and protein activity. Summary of the Invention

[0004] This invention provides a protein separation device, which aims to solve the problem of low efficiency in current hammer separation technology as mentioned in the background.

[0005] The present invention is implemented as follows: a protein separation device includes: a base plate; a separation tank disposed on the base plate; a tank lid disposed on the separation tank and capable of being opened and closed; a tamping mechanism mounted on the tank lid for tamping the material in the separation tank; a driving mechanism for driving the tamping mechanism to operate; and a filtering mechanism disposed on the base plate.

[0006] Preferably, the tamping mechanism includes: a slide rod slidably mounted on the can lid, with a hammer head fixed at the bottom end of the slide rod; an assembly plate fixedly connected to the can lid via a connecting block; a vertical plate fixed to the base plate, with a first guide rod fixed on the vertical plate, the first guide rod passing through the assembly plate; and a first electric telescopic rod fixed to the vertical plate, the push rod of the first electric telescopic rod being fixedly connected to the assembly plate for controlling the opening and closing of the can lid.

[0007] Preferably, the tamping mechanism further includes: a mounting bracket fixed to the assembly plate, on which a second guide rod is fixed; a sleeve plate slidably sleeved on the second guide rod, the bottom of the sleeve plate being fixedly connected to the top of the slide rod; a rotating shaft rotatably mounted on the mounting bracket via a bearing; and a cam fixed on the rotating shaft, the cam contacting the sleeve plate.

[0008] Preferably, the drive mechanism includes: a motor and a speed regulator fixed on the vertical plate, the output shaft of the motor being fixedly connected to the input shaft of the speed regulator via a coupling; a first transmission rod rotatably mounted on the vertical plate via bearings; a first bevel gear respectively fixed on the output shaft of the speed regulator and the first transmission rod and meshing with each other; and a second bevel gear fixed on the first transmission rod.

[0009] Preferably, the drive mechanism further includes: a second transmission rod rotatably mounted on the mounting plate; a third bevel tooth fixed to the bottom end of the second transmission rod, the third bevel tooth meshing with the second bevel tooth; a third transmission rod rotatably mounted on the mounting bracket; a fourth bevel tooth fixed to the second transmission rod and the third transmission rod respectively and meshing with each other; and a fifth bevel tooth fixed to the third transmission rod and the rotating shaft respectively and meshing with each other, for realizing the power transmission between the drive mechanism and the tamping mechanism.

[0010] Preferably, the filtration mechanism includes: a filter box disposed on the base plate; a first drain pipe fixedly connected to one side of the separation tank; a second drain pipe fixedly connected to one side of the filter box; valves respectively disposed on the first drain pipe and the second drain pipe; a filter box disposed in the filter box; and a filter screen disposed in the filter box for filtering the separated protein mixture.

[0011] Preferably, the separation tank is provided with a liquid guiding cavity for guiding coolant; a plurality of flow dividers are fixed in the liquid guiding cavity; an inlet pipe and an outlet pipe are fixed on the separation tank, both of which are connected to the liquid guiding cavity; the inlet pipe is used to connect to a coolant supply device for cooling the material in the separation tank to protect the activity of the protein.

[0012] Preferably, the vertical plate has a through groove, which is adapted to the first guide rod and the assembly plate.

[0013] Preferably, foot pads are symmetrically fixed to the bottom of the base plate, and a limiting block for limiting the position is fixed to the top of the second guide rod.

[0014] Preferably, a protective shell is fixed on the vertical plate, the protective shell is used to cover the motor, speed controller and first bevel gear, and a maintenance plate is fixed on the protective shell by bolts.

[0015] Compared with related technologies, the protein separation device provided by the present invention has the following advantages: The can lid opens and closes smoothly through the cooperation of the first electric telescopic rod, the assembly plate, and the first guide rod. The drive mechanism, consisting of a motor, speed regulator, and multiple sets of bevel gears and transmission rods, drives the hammer to perform automated tamping. The low-speed stirring of the stirring wheel enhances the mixing effect of the materials. The cooling design of the liquid guiding chamber and the flow divider reduces protein denaturation. The impurities are then filtered through the filter box and filter screen. The entire system reduces the intensity of manual operation and improves the efficiency and uniformity of protein separation through the coordinated operation of various mechanisms. The structural design facilitates sample loading, component maintenance, and impurity cleaning, making it suitable for small-scale experiments and precise processing needs. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the main structure of a protein separation device provided by the present invention; Figure 2 This is a schematic diagram of the main cross-sectional structure of a protein separation device provided by the present invention; Figure 3 for Figure 2 An enlarged structural diagram of part A shown in the figure; Figure 4 for Figure 2 An enlarged structural diagram of part B shown in the figure; Figure 5 for Figure 2 An enlarged structural diagram of section C shown in the figure; Figure 6 for Figure 2 An enlarged structural diagram of part D shown in the figure; Figure 7 This is a schematic diagram of the baffle structure in this invention; Figure 8 This is a schematic diagram of the structure of the storage box in this invention; Figure 9 This is a schematic diagram of the filter box in this invention.

[0017] Reference numerals: 1. Separating tank; 2. Tank cover; 3. Slide rod; 4. Hammer head; 5. Vertical plate; 6. First guide rod; 7. Assembly plate; 8. First electric telescopic rod; 9. Mounting bracket; 10. Second guide rod; 11. Sleeve plate; 12. Rotating shaft; 13. Cam; 14. Motor; 15. Speed ​​regulator; 16. First transmission rod; 17. First bevel gear; 18. Second bevel gear; 19. Second transmission rod; 20. Third bevel gear; 21. Third transmission rod; 2 2. Fourth conical tooth; 23. Fifth conical tooth; 24. Base plate; 25. Rotating rod; 26. Stirring wheel; 27. Spline tube; 28. Sixth conical tooth; 29. ​​Adjusting frame; 30. Spline rod; 31. Spline seat; 32. First drain pipe; 33. Filter box; 34. Second drain pipe; 35. Valve; 36. Filter box; 37. Filter screen bag; 38. Second electric telescopic rod; 39. Liquid guiding chamber; 40. Diverter plate; 41. Inlet pipe; 42. Outlet pipe. Detailed Implementation

[0018] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0019] This invention provides a protein separation device, such as... Figure 1-9 As shown, the protein separation device includes: a base plate 24; a separation tank 1 disposed on the base plate 24; a tank cover 2 disposed on the separation tank 1 and capable of being opened and closed; a tamping mechanism mounted on the tank cover 2 for tamping the material in the separation tank 1; a driving mechanism for driving the tamping mechanism to operate; and a filtering mechanism disposed on the base plate 24.

[0020] In this embodiment, when in use, the first electric telescopic rod 8 is activated to drive the assembly plate 7 to move upward along the first guide rod 6, so that the can lid 2 is opened and the pretreated biological sample and extraction buffer are put into the separation tank 1. Then, the can lid 2 is reset and closed by the first electric telescopic rod 8. At this time, the third bevel tooth 20 and the second bevel tooth 18 are engaged. The motor 14 is started, and after the speed is adjusted by the speed regulator 15, the first transmission rod 16 is driven to rotate through the first bevel gear 17. The second bevel gear 18, the third bevel gear 20, the second transmission rod 19, the fourth bevel gear 22, the third transmission rod 21, and the fifth bevel gear 23 drive the rotating shaft 12 to rotate. The cam 13 rotates accordingly and pushes the sleeve 11 to slide up and down along the second guide rod 10, which drives the slide rod 3 and the hammer head 4 to pound the material in the separation tank 1. After the tamping is completed, the valves 35 on the first drain pipe 32 and the second drain pipe 34 are opened. The material flows into the filter box 33 through the first drain pipe 32, is filtered through the filter screen 37 and discharged. The filter box 36 can be separated from the adjustment frame 29 for cleaning. The device realizes automated tamping and stirring through mechanical transmission. Combined with the cooling design of the liquid guiding chamber 39, it reduces the risk of protein denaturation. Compared with traditional manual operation, it improves separation efficiency and uniformity. The structural design facilitates sample loading and impurity removal.

[0021] In a further preferred embodiment of the present invention, the hammering mechanism includes: a slide rod 3 slidably mounted on the can lid 2, with a hammer head 4 fixed at the bottom end of the slide rod 3; an assembly plate 7 fixedly connected to the can lid 2 via a connecting block; a vertical plate 5 fixed on the base plate 24, with a first guide rod 6 fixed on the vertical plate 5, the first guide rod 6 penetrating the assembly plate 7; and a first electric telescopic rod 8 fixed on the vertical plate 5, the push rod of the first electric telescopic rod 8 being fixedly connected to the assembly plate 7 for controlling the opening and closing of the can lid 2.

[0022] In this embodiment, during use, the first electric telescopic rod 8 fixed on the vertical plate 5 is activated, and its push rod drives the assembly plate 7 to slide upward along the first guide rod 6 on the vertical plate 5. Since the assembly plate 7 is fixed to the can lid 2 through the connecting block, the can lid 2 on the separation can 1 is opened. The pretreated biological sample and extraction buffer are put into the separation can 1, and then the first electric telescopic rod 8 is controlled to reset, driving the assembly plate 7 and the can lid 2 to close. At this time, the third bevel tooth 20 and the second bevel tooth 18 mesh. After the starting motor 14 adjusts its speed through the speed regulator 15, it drives the rotating shaft 12 and cam 13 to rotate through the transmission structure. The cam 13 pushes the sleeve plate 11 to slide up and down along the second guide rod 10. The sleeve plate 11 drives the slide rod 3, which is slidably mounted on the tank cover 2, to move synchronously. This causes the hammer head 4, which is fixed at the bottom of the slide rod 3, to pound the material in the separation tank 1. During the process, the first guide rod 6 forms a guiding support for the assembly plate 7, ensuring the stability of the movement of the tank cover 2 and the pounding mechanism.

[0023] In a further preferred embodiment of the present invention, the tamping mechanism further includes: a mounting frame 9 fixed on the assembly plate 7, on which a second guide rod 10 is fixed; a sleeve plate 11 slidably sleeved on the second guide rod 10, the bottom of the sleeve plate 11 being fixedly connected to the top of the slide rod 3; a rotating shaft 12 rotatably mounted on the mounting frame 9 via a bearing; and a cam 13 fixed on the rotating shaft 12, the cam 13 contacting the sleeve plate 11.

[0024] In this embodiment, during use, the first electric telescopic rod 8 fixed on the vertical plate 5 is activated, and its push rod drives the assembly plate 7 to slide upward along the first guide rod 6 on the vertical plate 5. The assembly plate 7 drives the can lid 2 to open through the connecting block. The pretreated biological sample and extraction buffer are put into the separation can 1, and the first electric telescopic rod 8 is controlled to reset, driving the assembly plate 7 and the can lid 2 to close. At this time, the third bevel tooth 20 and the second bevel tooth 18 mesh, and the mounting bracket 9 on the assembly plate 7 is positioned synchronously with the assembly plate 7. After the starting motor 14 adjusts its speed through the speed regulator 15, it drives the rotating shaft 12 on the mounting frame 9 to rotate through the transmission structure. The rotating shaft 12 drives the cam 13 fixed on it to rotate synchronously. The cam 13 contacts the sleeve plate 11 that is slidably sleeved on the second guide rod 10 of the mounting frame 9, pushing the sleeve plate 11 to slide up and down along the second guide rod 10. The bottom of the sleeve plate 11 is fixed to the top of the slide rod 3, thereby driving the slide rod 3 that is slidably installed on the tank cover 2 and the hammer head 4 at the bottom to move, and to tamp the material in the separation tank 1. After the tamping is completed, the motor 14 is turned off, and the valves 35 on the first drain pipe 32 and the second drain pipe 34 are opened. The material is discharged after being filtered through the filter screen 37 in the filter box 33. The tamping mechanism integrates components such as the second guide rod 10 and the rotating shaft 12 through the mounting frame 9. The second guide rod 10 provides stable guidance for the sleeve plate 11. The cooperation between the cam 13 and the sleeve plate 11 makes the tamping action smoother. With the support of the first guide rod 6, the stability of the tamping operation is further improved, the assembly process of the mechanism is simplified, the intensity of manual operation is reduced, and the possibility of protein denaturation is reduced.

[0025] In a further preferred embodiment of the present invention, the driving mechanism includes: a motor 14 and a speed regulator 15 fixed on the vertical plate 5, wherein the output shaft of the motor 14 is fixedly connected to the input shaft of the speed regulator 15 via a coupling; a first transmission rod 16 rotatably mounted on the vertical plate 5 via bearings; a first bevel gear 17 fixed to the output shaft of the speed regulator 15 and the first transmission rod 16 respectively and meshing with each other; and a second bevel gear 18 fixed to the first transmission rod 16.

[0026] In this embodiment, when in use, the first electric telescopic rod 8 on the vertical plate 5 is activated, which drives the assembly plate 7 to slide upward along the first guide rod 6, thereby opening the can lid 2; the pretreated biological sample and extraction buffer are put into the separation tank 1, and the first electric telescopic rod 8 is controlled to reset and close the can lid 2. At this time, the second bevel tooth 18 and the third bevel tooth 20 on the first transmission rod 16 are engaged, and the motor 14 and the speed regulator 15 on the vertical plate 5 are in the ready-to-start state. The motor 14, fixed on the vertical plate 5, is started. Its output shaft drives the input shaft of the speed regulator 15 to rotate through the coupling. After the speed is adjusted by the speed regulator 15, the first bevel gear 17 on the output shaft of the speed regulator 15 drives the first transmission rod 16, which meshes with it and is rotatably mounted on the vertical plate 5, to rotate. The first transmission rod 16 synchronously drives the second bevel gear 18 fixed on it to rotate. Power is transmitted through the meshing of the bevel gears, which drives the rotating shaft 12 and cam 13 of the subsequent tamping mechanism to rotate, and finally realizes the tamping of the material by the hammer head 4.

[0027] In a further preferred embodiment of the present invention, the driving mechanism further includes: a second transmission rod 19 rotatably mounted on the mounting plate 7; a third bevel tooth 20 fixed to the bottom end of the second transmission rod 19, the third bevel tooth 20 meshing with the second bevel tooth 18; a third transmission rod 21 rotatably mounted on the mounting bracket 9; a fourth bevel tooth 22 respectively fixed to the second transmission rod 19 and the third transmission rod 21 and meshing with each other; and a fifth bevel tooth 23 respectively fixed to the third transmission rod 21 and the rotating shaft 12 and meshing with each other, for realizing the power transmission between the driving mechanism and the hammering mechanism.

[0028] In this embodiment, when in use, the first electric telescopic rod 8 on the vertical plate 5 is activated, which drives the assembly plate 7 to slide upward along the first guide rod 6, thereby opening the can lid 2; the pretreated biological sample and extraction buffer are put into the separation tank 1, and the first electric telescopic rod 8 is controlled to reset and close the can lid 2. At this time, the second bevel tooth 18 on the first transmission rod 16 meshes with the third bevel tooth 20 at the bottom of the second transmission rod 19 on the assembly plate 7, and the motor 14 and the speed regulator 15 are in the ready-to-start state; The motor 14 on the vertical plate 5 is started, and its output shaft drives the input shaft of the speed regulator 15 to rotate through the coupling. After the speed is adjusted by the speed regulator 15, the first bevel gear 17 on the output shaft of the speed regulator 15 drives the first transmission rod 16 to rotate. The first transmission rod 16 drives the second transmission rod 19 to rotate through the meshing of the second bevel gear 18 and the third bevel gear 20. The second transmission rod 19 drives the third transmission rod 21 on the mounting frame 9 to rotate through the fourth bevel gear 22. The third transmission rod 21 then drives the rotating shaft 12 to rotate through the fifth bevel gear 23, which finally drives the cam 13 and the hammer head 4 to rotate, thereby realizing the tamping of the material. After tamping is completed, the motor 14 is turned off, and the valves 35 on the first drain pipe 32 and the second drain pipe 34 are opened. The material is discharged after being filtered through the filter screen 37. The drive mechanism forms a complete transmission link through components such as the second transmission rod 19 and the third bevel gear 20. The bevel gear meshing design ensures smooth power transmission. With the speed regulator 15, the tamping speed can be flexibly adjusted to adapt to the processing needs of different materials. The overall transmission structure is compact, which reduces power loss and improves the stability of the mechanism. At the same time, it is easy to adjust the operating parameters according to the actual situation and reduce the possibility of protein denaturation.

[0029] In a further preferred embodiment of the present invention, the filtration mechanism includes: a filter box 33 disposed on the base plate 24; a first drain pipe 32 fixedly connected to one side of the separation tank 1; a second drain pipe 34 fixedly connected to one side of the filter box 33; valves 35 respectively disposed on the first drain pipe 32 and the second drain pipe 34; a filter box 36 disposed in the filter box 33; and a filter screen 37 disposed in the filter box 36 for filtering the separated protein mixture.

[0030] In this embodiment, before the hammering operation is started, ensure that the valves 35 on the first drain pipe 32 and the second drain pipe 34 are closed, the filter box 36 is placed stably in the filter box 33, the filter screen bag 37 in the filter box 36 is kept in the unfolded state, and the filter box 33 on the base plate 24 is in the corresponding communication position with the first drain pipe 32, in preparation for subsequent filtration. After the material in the separation tank 1 is processed by the tamping mechanism, the valves 35 on the first drain pipe 32 and the second drain pipe 34 are opened in sequence. The protein mixture in the separation tank 1 flows into the filter box 36 in the filter box 33 through the first drain pipe 32. It is filtered through the filter screen 37 to remove insoluble impurities such as tissue residue and cell fragments from the mixture. The filtered liquid is discharged through the second drain pipe 34 for subsequent separation and purification. After filtration is completed, valve 35 is closed, and filter box 36 in filter box 33 can be removed to clean or replace the filter screen 37. This filtration mechanism achieves impurity separation through the cooperation of filter box 36 and filter screen 37. The valves 35 of the first drain pipe 32 and the second drain pipe 34 facilitate the control of liquid flow. The overall structure is simple and easy to operate, which can reduce the interference of impurities on the subsequent separation process, improve the pretreatment effect of protein mixture, and the disassembly and assembly design of filter screen 37 facilitates maintenance and cleaning.

[0031] In a further preferred embodiment of the present invention, the separation tank 1 is provided with a liquid guiding cavity 39 for guiding coolant; a plurality of flow dividers 40 are fixed inside the liquid guiding cavity 39; an inlet pipe 41 and an outlet pipe 42 are fixed on the separation tank 1, and both the inlet pipe 41 and the outlet pipe 42 are connected to the liquid guiding cavity 39. The inlet pipe 41 is used to connect to a coolant supply device for cooling the material in the separation tank 1 to protect the activity of proteins.

[0032] In this embodiment, before starting the tamping operation, the inlet pipe 41 is connected to the external coolant supply equipment, the outlet pipe 42 is ensured to be unobstructed, the connectivity between the liquid guiding chamber 39 and the inlet pipe 41 and the outlet pipe 42 is checked, and it is confirmed that the biological sample and extraction buffer have been placed in the separation tank 1 and the tank lid 2 is in a closed state, in preparation for the cooling operation. While the drive mechanism is started to drive the tamping mechanism, the coolant supply equipment is turned on. The coolant flows into the liquid guiding chamber 39 on the separator tank 1 through the liquid inlet pipe 41. After being divided by several diverting plates 40 in the liquid guiding chamber 39, it flows evenly through the entire area of ​​the liquid guiding chamber 39 and exchanges heat with the material inside the separator tank 1. The coolant is discharged through the liquid outlet pipe 42 after cooling. The entire process accompanies the tamping operation. After the tamping operation is completed, first turn off the coolant supply equipment, then turn off the drive mechanism, and then proceed with the filtration operation according to the procedure. The cooling structure, through the cooperation of the liquid guiding chamber 39 and the diverter plate 40, makes the coolant evenly cover the outer periphery of the separation tank 1, improves the cooling efficiency, effectively reduces the impact of heat generated by mechanical friction during the tamping process on the protein, protects the protein activity, and the design of the liquid inlet pipe 41 and the liquid outlet pipe 42 facilitates the entry and exit of the coolant, and the structure is simple and easy to operate.

[0033] In a further preferred embodiment of the present invention, a through groove is provided on the vertical plate 5, and the through groove is adapted to the first guide rod 6 and the assembly plate 7.

[0034] In this embodiment, when the can lid 2 is opened, the first electric telescopic rod 8 is activated, and its push rod drives the assembly plate 7 to move upward along the first guide rod 6. The through groove on the vertical plate 5 provides clearance for the movement of the assembly plate 7, avoiding interference between the assembly plate 7 and the vertical plate 5. When the can lid 2 is closed, the first electric telescopic rod 8 resets and drives the assembly plate 7 to slide downward. The through groove, together with the first guide rod 6, forms a limiting guide for the assembly plate 7, ensuring that the can lid 2 is accurately closed. The through-slot structure is designed to be compatible with the first guide rod 6 and the assembly plate 7, providing stable support and clearance for the up-and-down movement of the assembly plate 7, reducing jamming during the movement of the mechanism, improving the smoothness of the opening and closing operation of the can lid 2, and at the same time helping to ensure the accuracy of the meshing of each bevel tooth after the can lid 2 is closed.

[0035] In a further preferred embodiment of the present invention, foot pads are symmetrically fixed to the bottom of the base plate 24, and a limiting block for limiting the position is fixed to the top of the second guide rod 10.

[0036] In this embodiment, when the device is running, the foot pads at the bottom of the base plate 24 provide support for the device, reduce the transmission of vibrations generated during operation, and maintain the positional stability of components such as the separation tank 1 and the vertical plate 5; when the hammering mechanism is working, the sleeve plate 11 slides up and down along the second guide rod 10, and the limiting block at the top of the second guide rod 10 restricts the stroke of the sleeve plate 11, preventing the sleeve plate 11 from disengaging from the second guide rod 10.

[0037] In a further preferred embodiment of the present invention, a protective shell is fixed on the vertical plate 5. The protective shell is used to cover the motor 14, the speed regulator 15 and the first bevel gear 17. A maintenance plate is fixed on the protective shell by bolts.

[0038] In this embodiment, the protective shell protects the motor 14, speed controller 15 and first bevel gear 17, reducing the contamination of transmission components by external dust and debris, and preventing personnel from accidentally touching the operating components; when it is necessary to inspect or maintain the internal components, the bolts can be unscrewed and the inspection plate removed to operate the motor 14, speed controller 15 or first bevel gear 17.

[0039] To further improve the performance of this device, in addition to the above-mentioned solutions, this solution also includes the following embodiments: In another embodiment of the present invention, the stirring mechanism includes: a rotating rod 25 rotatably mounted at the bottom of the separation tank 1 via a sealed bearing, the top end of the rotating rod 25 extending into the separation tank 1 and fixed with a stirring wheel 26; a spline tube 27 rotatably mounted at the bottom of the separation tank 1 via a bearing seat; a sixth bevel tooth 28 fixed to the rotating rod 25 and the spline tube 27 respectively and meshing with each other; an adjusting frame 29 disposed below the separation tank 1 and adjustable laterally, one end of the adjusting frame 29 being inserted into the filter box 33 and fixedly connected to the filter box 36; a second bevel tooth 18 and a spline seat 31 fixed on the first transmission rod 16; a spline rod 30 rotatably mounted on the adjusting frame 29 via a bearing, one end of the spline rod 30 penetrating the spline tube 27 and being able to be inserted into the spline seat 31 for transmission; and a second electric telescopic rod 38 fixed on the filter box 33, the push rod of the second electric telescopic rod 38 being fixedly connected to the adjusting frame 29 and used to control the engagement and disengagement of the spline rod 30 and the spline seat 31 to achieve low-speed rotation of the stirring wheel 26 to assist separation.

[0040] In this embodiment, before the tamping operation is started, ensure that the adjusting frame 29 is in the initial position and the spline rod 30 is not inserted into the spline seat 31; after the can lid 2 is closed, start the second electric telescopic rod 38 on the filter box 33, and its push rod pushes the horizontally adjustable adjusting frame 29 to move, causing the spline rod 30 on the adjusting frame 29 to slide along the spline tube 27, and finally insert into the spline seat 31 on the first transmission rod 16 to complete the power transmission connection. At the same time, the adjusting frame 29 drives the filter box 36 to be in the appropriate position in the filter box 33. When the drive mechanism rotates the first transmission rod 16, the spline seat 31 drives the spline tube 27 to rotate via the spline rod 30. The spline tube 27 drives the rotating rod 25 to rotate via the sixth bevel tooth 28. The stirring wheel 26 at the top of the rotating rod 25 rotates at a low speed in the separation tank 1. The stirring wheel 26, in conjunction with the hammering action of the hammer head 4, stirs the material, making the material fully mixed with the extraction buffer, and at the same time promoting the release of proteins after cell rupture. After the hammering is completed, the second electric telescopic rod 38 is activated to reset, driving the adjusting frame 29 and the spline rod 30 to retract, and the spline rod 30 separates from the spline seat 31. The stirring mechanism uses a second electric telescopic rod 38 to control the engagement and disengagement of the spline rod 30 and the spline seat 31. Power is taken from the first transmission rod 16, eliminating the need for additional drive components and simplifying the structure. The low-speed rotation of the stirring wheel 26 improves the uniformity of material mixing and facilitates protein release. Combined with the tamping action, it enhances the separation effect. At the same time, the adjusting frame 29 is fixedly connected to the filter box 36, and the position of the filter box 36 is adjusted synchronously during the engagement and disengagement process to prepare for subsequent filtration, thus improving the continuity and practicality of the device operation.

[0041] In summary, compared with related technologies, the smooth opening and closing of the can lid 2 is achieved through the cooperation of the first electric telescopic rod 8, the assembly plate 7, and the first guide rod 6. The drive mechanism, consisting of the motor 14, the speed regulator 15, and multiple sets of bevel gears and transmission rods, drives the hammer head 4 to perform automated tamping. The low-speed stirring of the stirring wheel 26 improves the mixing effect of materials. The cooling design of the liquid guiding chamber 39 and the flow divider 40 reduces protein denaturation. The impurities are then filtered through the filter box 33 and the filter screen 37. The overall system reduces the intensity of manual operation and improves the efficiency and uniformity of protein separation through the coordinated operation of various mechanisms. Moreover, the structural design facilitates sample loading, component maintenance, and impurity cleaning, making it suitable for small-scale experiments and precise processing needs.

[0042] It is worth noting that in practical applications, the operating parameters of this device can be flexibly adjusted according to the characteristics of biological samples to adapt to the separation needs of different types of materials: for samples with thick cell walls such as plant leaves and stems, the hammering frequency can be adjusted by the speed regulator 15 to control the speed of the stirring wheel 26 to ensure that the cell walls are fully broken; in the cooling stage, the temperature of the coolant can be adjusted to 0-4℃ according to the temperature sensitivity of the sample, and the temperature inside the separation tank 1 can be kept stable by controlling the flow rate of the coolant (5-10mL / min), which is especially suitable for the separation of heat-sensitive proteins such as enzymes and antibodies, and effectively reduces the denaturation rate.

[0043] In terms of maintenance and cleaning, all key components of the device adopt a modular design: the filter bag 37 is made of food-grade nylon, which can be disassembled and rinsed with clean water or ultrasonically cleaned, and can be reused more than 50 times; the inner wall of the separation tank 1 is polished, smooth and without dead corners. After tamping, cleaning fluid can be injected through the inlet pipe 41, the stirring mechanism can be started to clean the inner wall, and the waste liquid can be discharged through the drain pipe. Cleaning can be completed without disassembly, reducing the risk of cross-contamination. The inspection plate design of the protective shell facilitates regular inspection of the operating status of the motor 14, speed regulator 15 and bevel gear transmission components. If transmission jamming or power loss occurs, it can be quickly disassembled for repair.

[0044] Furthermore, this device can form a highly efficient synergy with subsequent separation equipment and is adaptable to multi-process workflows: the filtered supernatant can be directly connected to a centrifuge tube through the second drain pipe 34 for low-speed centrifugation to further remove impurities; it can also be connected to the chromatography column inlet, eliminating intermediate transfer steps and improving operational continuity. Through the above design, this device effectively solves the problems of poor adaptability, complex operation, and high maintenance costs of traditional separation equipment, providing an efficient, convenient, and accurate technical solution for protein separation in fields such as bioengineering and pharmaceutical research and development. The circuits, electronic components, and modules involved in this invention are all existing technologies, which can be fully implemented by those skilled in the art, and need not be elaborated upon. The content protected by this invention does not involve improvements to the software and methods.

[0045] It should be understood, in the several embodiments provided in this application, that the disclosed apparatus may be implemented in other ways.

[0046] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit the scope of protection of the invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on these embodiments, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art can still combine, add, delete, or otherwise adjust the features of the various embodiments of the present invention according to the circumstances without conflict or creative effort, thereby obtaining different technical solutions that do not depart from the concept of the present invention. These technical solutions are also within the scope of protection of the present invention.

Claims

1. A protein separation device, characterized in that, include: Base plate; Separation tank disposed on the base plate; A can lid that is installed on the separation tank and can be opened and closed; The tamping mechanism mounted on the tank cover is used to tamp the material inside the separation tank; The drive mechanism is used to drive the tamping mechanism. The filter mechanism is installed on the base plate.

2. The protein separation apparatus as described in claim 1, characterized in that, The tamping mechanism includes: A sliding rod is slidably mounted on the can lid, and a hammer is fixed to the bottom end of the sliding rod; An assembly plate that is fixedly connected to the can lid via a connecting block; A vertical plate is fixed to the base plate, and a first guide rod is fixed on the vertical plate, the first guide rod passing through the assembly plate; The first electric telescopic rod is fixed to the vertical plate, and the push rod of the first electric telescopic rod is fixedly connected to the assembly plate for controlling the opening and closing of the can lid.

3. The protein separation apparatus as described in claim 2, characterized in that, The tamping mechanism also includes: A mounting bracket fixed to an assembly plate, wherein a second guide rod is fixed on the mounting bracket; A sleeve plate is slidably fitted onto the second guide rod, with the bottom of the sleeve plate fixedly connected to the top of the guide rod; The rotating shaft mounted on the mounting bracket rotates via bearings; A cam is fixed on the rotating shaft, and the cam contacts the sleeve plate.

4. The protein separation apparatus as described in claim 3, characterized in that, The drive mechanism includes: A motor and a speed controller are fixed on the vertical plate, and the output shaft of the motor is fixedly connected to the input shaft of the speed controller through a coupling; The first transmission rod is rotatably mounted on the vertical plate via a bearing; First bevel teeth, respectively fixed to the output shaft of the speed regulator and the first transmission rod and meshing with each other; The second bevel tooth is fixed on the first transmission rod.

5. The protein separation apparatus as described in claim 4, characterized in that, The drive mechanism also includes: Rotate the second transmission rod mounted on the assembly plate; A third bevel tooth is fixed to the bottom end of the second transmission rod, and the third bevel tooth meshes with the second bevel tooth; Rotate the third transmission rod mounted on the mounting bracket; A fourth bevel tooth, which is fixed to the second transmission rod and the third transmission rod respectively and meshes with each other; The fifth bevel teeth are fixed to the third transmission rod and the rotating shaft respectively and mesh with each other.

6. The protein separation apparatus as described in claim 1, characterized in that, The filtration mechanism includes: The filter box is installed on the base plate; A first drain pipe is fixedly connected to one side of the separation tank; A second drain pipe is fixedly connected to one side of the filter box; Valves respectively installed on the first drain pipe and the second drain pipe; Filter box installed inside the filter box; The filter screen bag installed inside the filter box is used to filter the separated protein mixture.

7. The protein separation apparatus as described in claim 1, characterized in that, The separator tank is provided with a liquid guiding cavity for guiding coolant; a number of flow dividers are fixed inside the liquid guiding cavity; an inlet pipe and an outlet pipe are fixed on the separator tank, and both the inlet pipe and the outlet pipe are connected to the liquid guiding cavity. The inlet pipe is used to connect to the coolant supply equipment.

8. The protein separation apparatus as described in claim 2, characterized in that, The vertical plate has a through groove, which is adapted to the first guide rod and the assembly plate.

9. The protein separation apparatus as described in claim 3, characterized in that, The bottom of the base plate is symmetrically fixed with foot pads, and the top of the second guide rod is fixed with a limiting block for limiting the position.

10. The protein separation apparatus as described in claim 4, characterized in that, A protective shell is fixed to the vertical plate. The protective shell is used to cover the motor, speed controller and first bevel gear. A maintenance plate is fixed to the protective shell by bolts.

Images