Biomedical detection chip

Abstract

A biomedical detection chip comprises a first detection module and a second detection module located below the first detection module, the first detection module comprises a first substrate and at least one first flow channel unit formed on the first substrate, and the first flow channel unit comprises a first flow channel located in the first substrate. The first detection module comprises a first substrate and at least one first flow channel unit, and a first opening and a second opening positioned at two ends of the first flow channel, the second detection module comprises a second substrate and at least one second flow channel unit corresponding to the first flow channel units in number, and the second flow channel unit comprises a second flow channel positioned in the second substrate; the third opening and the fourth opening are located at the two ends of the first flow channel, and the first substrate does not shield the fourth opening. And through the design of stacking a plurality of detection modules, the effect of simultaneously detecting multiple organ cells can be achieved.

Description

Technical Field

[0001] This invention relates to a detection chip, and more particularly to a biomedical detection chip for cell detection. Background Technology

[0002] The development of modern technology has brought convenience to people's lives. However, this convenience has also led to a faster pace of life, coupled with societal pressures and changes in dietary habits, resulting in a host of diseases. The number of people suffering from cancer is increasing year by year, and in addition to the previously believed family genetic factors, more and more patients are succumbing to their daily lifestyle habits.

[0003] In recent years, medical testing technology has been continuously developing. Compared to the past, when developing new therapeutic drugs, animal experiments were used to observe the effects and side effects of the drugs. This was not only time-consuming and costly in terms of research and development, but also sometimes relied on doctors' past medical experience for judgment in actual medical procedures. The emergence of biomedical testing chips has gradually replaced the past methods of directly conducting animal or human experiments due to their advantages such as reducing test dosage, shortening testing time, avoiding the use of animal experiments, and simulating human organ cells.

[0004] However, most biomedical testing chips currently on the market can only test one type of organ and cell at a time. To enable doctors to have a comprehensive understanding of the anticipated situation before treating patients, thereby increasing the chances of a cure, it is necessary to develop chips that can simultaneously detect multiple drugs or even test multiple organs at the same time, thus achieving the goal of personalized precision medicine. Summary of the Invention

[0005] The purpose of this invention is to provide a biomedical detection chip that can simultaneously detect cells from multiple organs.

[0006] The biomedical detection chip of the present invention includes a first detection module and a second detection module.

[0007] The first detection module includes a first substrate and at least one first flow channel unit formed on the first substrate.

[0008] The first detection module includes a first substrate and at least one first flow channel unit formed on the first substrate.

[0009] The first flow channel unit includes a first flow channel located within the first substrate, a first opening extending downward from the surface of the first substrate and communicating with one end of the first flow channel, a first chamber located within the first substrate and communicating with the other end of the first flow channel, and a second opening extending downward from the surface of the first substrate, located downstream of the first chamber and communicating with the first chamber.

[0010] The second detection module is located below the first detection module and includes a second substrate and at least one second flow channel unit corresponding to the number of the first flow channel units.

[0011] The second flow channel unit includes a second flow channel located within the second substrate, a third opening extending downward from the surface of the second substrate and communicating with two opposite ends of the second flow channel, and a fourth opening.

[0012] The second flow channel has a second chamber between the third opening and the fourth opening, the second opening corresponding to the third opening and communicating with each other, and the first substrate does not block the fourth opening.

[0013] Preferably, in the biomedical detection chip of the present invention, the first chamber has a plurality of micropillars distributed therein.

[0014] Preferably, in the biomedical detection chip of the present invention, at least a portion of the first flow channel has a serrated structure.

[0015] Preferably, the biomedical detection chip of the present invention further includes a feeding module located on the first detection module. The feeding module includes a feeding substrate and at least one feeding port formed downward from the surface of the feeding substrate and communicating with the first opening, and the feeding substrate does not cover the second opening.

[0016] Preferably, the biomedical detection chip of the present invention further includes an electrode module located below the second detection module. The electrode module includes an electrode carrier plate and at least one electrode pattern unit formed on the surface of the electrode carrier plate and corresponding to the second chamber. The electrode pattern unit has a first electrode group and a second electrode group arranged in a predetermined pattern.

[0017] Preferably, in the biomedical detection chip of the present invention, the first detection module includes a plurality of first flow channel units, the second detection module includes a plurality of second flow channel units corresponding to the first flow channel units, and the first flow channels have the same length.

[0018] Preferably, the biomedical detection chip of the present invention further includes a feeding module located on the first detection module. The feeding module includes a feeding substrate and a feeding distribution unit. The feeding distribution unit has multiple feeding ports, multiple inlet ports, and multiple communicating channels. The feeding ports are formed downward from the surface of the feeding substrate and correspond to the positions of the first opening and communicate with each other. The communicating channels are located in the feeding substrate and connect the feeding ports and the inlet ports. The feeding substrate does not cover the second opening.

[0019] Preferably, in the biomedical detection chip of the present invention, the first detection module further includes a first control channel unit, and the second detection module further includes a second control channel unit. The first control channel unit has a first control channel located within the first substrate, and a first inlet and a first outlet extending downward from the surface of the first substrate and respectively communicating with the first control channel. The first control channel has a first control chamber located between the first inlet and the first outlet, and the first control chamber has a plurality of micropillars distributed thereon. The second control channel unit has a second control channel located within the second substrate, and a second inlet and a second outlet extending downward from the surface of the second substrate and respectively communicating with the second control channel. The second control channel has a second control chamber located between the second inlet and the second outlet, and the first outlet and the second inlet are correspondingly communicated with each other.

[0020] Preferably, the biomedical detection chip of the present invention further includes an electrode module located below the second detection module. The electrode module includes an electrode carrier plate and a plurality of electrode pattern units formed on the surface of the electrode carrier plate and corresponding to the second chamber. The electrode pattern units have a first electrode group and a second electrode group arranged in a predetermined pattern.

[0021] Preferably, the biomedical detection chip of the present invention further includes an inlet / outlet tube unit, the inlet / outlet tube unit including a plurality of inlet / outlet tubes corresponding to the second opening, the fourth opening and the inlet and extending upward, and the inlet / outlet tubes communicating with the corresponding second opening, the fourth opening and the inlet.

[0022] The beneficial effect of the present invention is that by arranging the first detection module and the second detection module in a stacked structure, and designing the corresponding first flow channel unit and the second flow channel unit, it is possible to achieve the purpose of simultaneously detecting multiple organ cells. Attached Figure Description

[0023] Figure 1 This is an exploded three-dimensional view illustrating an embodiment of the biomedical detection chip of the present invention;

[0024] Figure 2 This is a top view illustrating the first detection module of the embodiment;

[0025] Figure 3 This is a top view illustrating the second detection module of the embodiment;

[0026] Figure 4 This is a top view illustrating the electrode module of the embodiment; and

[0027] Figure 5 This is a top view illustrating the feeding module of the embodiment. Detailed Implementation

[0028] The present invention will now be described in detail with reference to the accompanying drawings and embodiments.

[0029] Before the present invention is described in detail, it should be noted that similar components are represented by the same numbers in the following description.

[0030] The relevant technical content, features, and effects of this invention will be clearly presented in the following detailed description of the embodiments with reference to the accompanying drawings. Furthermore, it should be noted that the drawings of this invention are only for illustrating the structural and / or positional relationships between components and are not related to the actual dimensions of each component.

[0031] See Figures 1 to 5 An embodiment of the biomedical detection chip of the present invention includes a first detection module 2, a second detection module 3, an electrode module 4, a feeding module 5, and an inlet / outlet tube unit 6.

[0032] The first detection module 2 includes a first substrate 21, multiple sets of first flow channel units 22 formed on the first substrate 21, and a first comparison unit 23.

[0033] The first flow channel unit 22 includes a first flow channel 221 located within the first substrate 21 and at least partially having a sawtooth structure, a first opening 222 extending downward from the surface of the first substrate 21 and communicating with one end of the first flow channel 221, a first chamber 223 located within the first substrate 21 and communicating with the other end of the first flow channel 221, and a second opening 225 extending downward from the surface of the first substrate 21, located downstream of the first chamber 223 and communicating with the first chamber 223, and the first chamber 223 also has a plurality of distributed micropillars 224.

[0034] The first flow channels 221 have the same length so that the liquid flowing through the first flow channels 221 can generate the same flow resistance. In addition, by making the first flow channels 221 serrated and by providing a plurality of micropillars 224 in the first chamber 223, the purpose is to enable the liquid flowing through the first flow channels 221 and entering the first chamber 223 to have a better mixing and dispersion effect.

[0035] Preferably, the diameter of the micropillars 224 is between 0.05 and 0.1 micrometers, which can have a better dispersion effect without affecting the fluidity of the liquid.

[0036] This embodiment uses a micropillar 224 with a diameter of 0.05 micrometers as an example, but it is not limited to this.

[0037] In some embodiments, the first flow channel 221 may be planar as needed, without needing to be serrated. Furthermore, in some embodiments, the first chamber 223 may be provided without the micropillars 224.

[0038] The first control unit 23 has a first control channel 231 located in the first substrate 21, and a first inlet 232 and a first outlet 233 extending downward from the surface of the first substrate 21 and respectively connecting the opposite ends of the first control channel 231. The first control channel 231 has a first control chamber 234 between the first inlet 232 and the first outlet 233, and the first control chamber 234 also has a plurality of micropillars 235 that are the same as the micropillars 224 distributed in the first chamber 223.

[0039] The second detection module 3 is located below the first detection module 2, and includes a second substrate 31 connected to the first substrate 21 and located below the first substrate 21, multiple sets of second flow channel units 32 corresponding to the number of the first flow channel units 22, and a second comparison unit 33.

[0040] The second flow channel unit 32 includes a second flow channel 321 located in the second substrate 31, a third opening 322 extending downward from the surface of the second substrate 31 and communicating with the two opposite ends of the second flow channel 321, and a fourth opening 323.

[0041] The second flow channel 321 has a second chamber 324 between the third opening 322 and the fourth opening 323. The second opening 225 of the first detection module 2 corresponds to the third opening 322 and is connected to each other. The first substrate 21 does not block the fourth opening 323.

[0042] The second control unit 33 has a second control channel 331 located within the second substrate 31, and a second inlet 332 and a second outlet 333 extending downward from the surface of the second substrate 31 and respectively connecting the two opposite ends of the second control channel 331. The second control channel 331 has a second control chamber 334 located between the second inlet 332 and the second outlet 333, and the first outlet 233 of the first control channel 231 is correspondingly connected to the second inlet 332.

[0043] The feed pipe unit 6 includes a plurality of feed pipes 61, which are provided and extend upwards corresponding to the second opening 225, the fourth opening 323, and the feed inlet 521. The feed pipes 61 are connected to the corresponding second opening 225, the fourth opening 323, and the feed inlet 521 for filling or removing liquid from the feed pipes 61.

[0044] The electrode module 4 is located below the second detection module 3 and includes an electrode carrier plate 41 connected to and located below the second substrate 31, and multiple sets of electrode pattern units 42 formed on the surface of the electrode carrier plate 41 and corresponding to the second chamber 324. Each electrode pattern unit 42 has a first electrode group 421 and a second electrode group 422 arranged in a predetermined pattern. The first electrode group 421 and the second electrode group 422 each form an external electrical connection line to form a first electrode circuit and a second electrode circuit. Through the voltage difference generated between the first electrode circuit and the second electrode circuit, the organ cells located in the second chamber 324 can be evenly distributed and arranged into a pattern corresponding to the electrode pattern unit 42, facilitating more accurate detection of the actual state of the organ cells.

[0045] It should be noted that, Figure 4 Taking the first electrode group 421 and the second electrode group 422 of the electrode pattern unit 42 as an example, each has multiple sub-electrodes, and the sub-electrodes are arranged in a hexagonal pattern. However, in actual implementation, the patterns of the first electrode group 421 and the second electrode group 422 can be adjusted as needed and are not limited to this.

[0046] The feeding module 5 is located above the first detection module 2 and includes a feeding substrate 51 and a feeding distribution unit 52.

[0047] The feed substrate 51 is connected to the surface of the first substrate 21 and is located on the first substrate 21, and the feed substrate has a surface and a bottom surface that are opposite to each other.

[0048] The feeding distribution unit 52 has a plurality of feeding ports 521 formed downward from the surface of the feeding substrate 51, a plurality of feeding ports 522 formed from the bottom surface of the feeding substrate 51 toward the surface and corresponding to and communicating with the first opening 222, and a plurality of communicating channels 523 located inside the feeding substrate 51 to connect the feeding ports 521 and the feeding ports 522, and the feeding substrate 51 does not cover the second opening 225. In this embodiment, the feeding distribution unit 52 has three feeding ports 521, with the feeding ports 521 located at the apex of the triangle, and three feeding ports 522 located outside the three sides of the triangle and three feeding ports 522 located inside the triangle and opposite to the feeding ports 521. The communicating channels 523 are used to connect the feeding ports 521 and the feeding ports 522 respectively. However, in actual implementation, as long as the distribution design of the feed inlet 521, the feed port 522, and the connecting channel 523 allows different liquids entering from the feed inlet 521 to form mixtures of different mixing states through the interconnected distribution of the connecting channel 523, the mixtures can enter the first detection module 2 from the feed port 522. This is not limited to... Figure 5 The structure and quantity are shown.

[0049] Furthermore, it should be noted that the feed substrate 51, the first substrate 21, and the second substrate 31 of the present invention are stacked in a stepped manner. Therefore, the feed distribution unit 52, the first flow channel unit 22, and the second flow channel unit 32 located in the feed substrate 51, the first substrate 21, and the second substrate 31 will present a stepped discontinuity structure at the connecting nodes. Therefore, the air bubbles generated when the injected liquid flows will be blocked and stay at the position of the discontinuity structure and will not flow arbitrarily, thereby reducing the problem of interfering with the subsequent flow of the liquid or affecting the detection results.

[0050] Preferably, the first substrate 21, the second substrate 31 and the feed substrate 51 are made of a soft silicone material with good biocompatibility.

[0051] When using the biomedical detection chip of this invention for multi-organ cell detection, the inlet / outlet tube 61, which communicates with the fourth opening 323, is first sealed. A first mixture comprising a photocurable hydrogel and a first type of cell is injected through the second opening 225, filling the first chamber 223 with the first mixture. Next, the first mixture within the first chamber 223 is cured by ultraviolet light to immobilize the first type of cell within the first chamber 223. The remaining first mixture outside the first chamber 223 is rinsed with buffer solution injected through the inlet 521 and removed through the fourth opening 323, completing the capture of the first type of cell in the first chamber 223.

[0052] Next, the inlet / outlet pipe 61, which is connected to the second opening 225, is sealed. A buffer solution containing the second type of cells is injected through the fourth opening 323. Once the second type of cells adhere to the second chamber 324, the buffer solution injected through the inlet 521 is used to rinse away any excess second type of cells. This step is repeated once, but instead of injecting, a second mixture consisting of hydrogel and the third type of cells is injected. The second mixture located in the second chamber 324 is then cured by ultraviolet light to fix the second type of cells in the second chamber 324. Any excess second mixture is rinsed away by the buffer solution injected through the inlet 521 and removed through the fourth opening 323, thus completing the capture of the second type of cells in the second chamber 324.

[0053] The aforementioned water-based adhesive contains biocompatible polymers and compounds used for photocuring and polymer crosslinking. Since the materials and related components of the water-based adhesive and buffer solution are known in the relevant technical field, they will not be described in detail here.

[0054] After capturing the first and second types of cells, three types of drugs to be tested are added through the three inlets 521. The three drugs can be mixed and arranged through the connecting channel 523 to form six different drug combinations, which then flow into the first detection module 2 through the six inlets 522 to complete the drug distribution and injection.

[0055] The different drug combinations flow sequentially from the first opening 222 through the first channel 221, the first chamber 223 to the second opening 225, and then through the third opening 322, the second channel 321 and the second chamber 324 of the second detection module 3, so that the drugs fill the entire first detection module 2 and the second detection module 3.

[0056] During the testing process, the drug is continuously injected through the inlet 521, and liquid can be drawn from the inlet / outlet pipes 61 of the second opening 225 and the fourth opening 323 for testing, thereby achieving the functions of drug combination screening and simultaneous detection of multiple organ cells. After the operation is completed, all liquid is discharged through the fourth opening 323.

[0057] Furthermore, before injecting the cell-containing buffer solution into the second chamber 324, the first electrode group 421 and the second electrode group 422 of the electrode module 4 can be additionally connected to form a first electrode circuit and a second electrode circuit. The electric field difference generated between the first electrode circuit and the second electrode circuit allows for a uniform distribution of the organ cells in the second chamber 324. Designing the patterns of the first electrode group 421 and the second electrode group 422 can yield more accurate detection results. For example, as shown in the embodiment, the first electrode group 421 and the second electrode group 422 can be designed as an electrode pattern consisting of multiple sub-electrodes arranged in a hexagonal shape, mimicking the shape of liver lobules. This allows liver cells to also be arranged in a hexagonal distribution along with the electrode pattern during the detection process, thus more accurately simulating the arrangement of human organ cells to obtain more accurate detection results.

[0058] In some embodiments, the electrode module 4 and the feeding module 5 may be omitted as needed, and the same effect can be achieved simply by combining the first detection module 2 and the second detection module 3.

[0059] In some embodiments, in order to confirm the extent of the effect of the drug combination on the organ cells in the first chamber 223 and the second chamber 324, the organ cells are captured according to the above process through the first control unit 23 and the second control unit 33, serving as controls for the first flow channel unit 22 and the second flow channel unit 32.

[0060] It should be noted that the embodiments described in this invention use multiple sets of the first flow channel units 22 as an example. However, in actual implementation, the first flow channel 221 may only require one set. When the first flow channel 221 is one set, the other cooperating first chambers 223, first openings 222, and second openings 225 can also be configured as one set. Furthermore, the second flow channel unit 32, the electrode pattern unit 42, and the inlet / outlet pipe 61 can also be configured as one set, which can also achieve the effect of simultaneously detecting cells from multiple organs.

[0061] In summary, the biomedical detection chip of the present invention, through the combination of the first chamber 223 of the first detection module 2 and the second chamber 324 of the second detection module 3, along with the design of the feed dispensing unit 52, enables the device to simultaneously detect different combinations of drugs and two different types of cells respectively captured by the first chamber 223 and the second chamber 324. Furthermore, due to the stepped design of the substrate, air bubbles generated during the detection process do not affect the detection results, thus effectively achieving the objectives of the present invention.

[0062] However, the above description is merely an embodiment of the present invention and should not be construed as limiting the scope of the present invention. Any simple equivalent changes and modifications made in accordance with the scope of the patent application and the contents of the patent specification shall still fall within the scope of the patent of the present invention.

Claims

1. A biomedical detection chip, characterized in that, Include: A first detection module includes a first substrate and at least one first flow channel unit formed on the first substrate. The first flow channel unit includes a first flow channel located within the first substrate, a first opening extending downwards from the surface of the first substrate and communicating with one end of the first flow channel, a first chamber located within the first substrate and communicating with the other end of the first flow channel, and a second opening extending downwards from the surface of the first substrate, downstream of the first chamber and communicating with the first chamber. The second detection module, located below the first detection module, includes a second substrate and at least one second flow channel unit corresponding to the number of the first flow channel units. The second flow channel unit includes a second flow channel located within the second substrate, a third opening extending downward from the surface of the second substrate and communicating with the two opposite ends of the second flow channel, and a fourth opening. The second flow channel has a second chamber between the third opening and the fourth opening. The second opening corresponds to the third opening and communicates with each other, and the first substrate does not obscure the fourth opening.

2. The biomedical detection chip according to claim 1, characterized in that, The first chamber contains a plurality of micropillars arranged in a distributed manner.

3. The biomedical detection chip according to claim 1, characterized in that, At least a portion of the first flow channel has a serrated structure.

4. The biomedical detection chip according to claim 1, characterized in that, It also includes a feeding module located on the first detection module. The feeding module includes a feeding substrate and at least one feeding port formed downward from the surface of the feeding substrate and communicating with the first opening. The feeding substrate does not cover the second opening.

5. The biomedical detection chip according to claim 1, characterized in that, It also includes an electrode module located below the second detection module. The electrode module includes an electrode carrier plate and at least one electrode pattern unit formed on the surface of the electrode carrier plate and corresponding to the second chamber. The electrode pattern unit has a first electrode group and a second electrode group arranged in a predetermined pattern.

6. The biomedical detection chip according to claim 1, characterized in that, The first detection module includes multiple first flow channel units, and the second detection module includes multiple second flow channel units corresponding to the first flow channel units, wherein the first flow channels have the same length.

7. The biomedical detection chip according to claim 6, characterized in that, It also includes a feeding module located on the first detection module. The feeding module includes a feeding substrate and a feeding distribution unit. The feeding substrate has a surface and a bottom surface that are opposite to each other. The feeding distribution unit has a plurality of feeding ports, a plurality of inlet ports, and a plurality of connecting channels. The feeding ports are formed downward from the surface of the feeding substrate and the inlet ports are formed from the bottom surface of the feeding substrate toward the surface. They correspond to the positions of the first opening and are connected to each other. The connecting channels are located inside the feeding substrate and connect the feeding ports and the inlet ports. The feeding substrate does not cover the second opening.

8. The biomedical detection chip according to claim 6, characterized in that, The first detection module further includes a first control channel unit, and the second detection module further includes a second control channel unit. The first control channel unit has a first control channel located within the first substrate, and a first inlet and a first outlet extending downward from the surface of the first substrate and respectively communicating with the first control channel. The first control channel has a first control chamber located between the first inlet and the first outlet, and the first control chamber has a plurality of micropillars distributed thereon. The second control channel unit has a second control channel located within the second substrate, and a second inlet and a second outlet extending downward from the surface of the second substrate and respectively communicating with the second control channel. The second control channel has a second control chamber located between the second inlet and the second outlet, and the first outlet and the second inlet are correspondingly connected to each other.

9. The biomedical detection chip according to claim 7, characterized in that, It also includes an electrode module located below the second detection module. The electrode module includes an electrode carrier plate and a plurality of electrode pattern units formed on the surface of the electrode carrier plate and corresponding to the second chamber. The electrode pattern units have a first electrode group and a second electrode group arranged in a predetermined pattern.

10. The biomedical detection chip according to claim 4 or 7, characterized in that, It also includes an inlet / outlet pipe unit, which includes a plurality of inlet / outlet pipes corresponding to the second opening, the fourth opening, and the feed inlet and extending upward therefrom, and the inlet / outlet pipes are connected to the corresponding second opening, the fourth opening, and the feed inlet.

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