Device and method for efficiently detecting bactericide residues in grains

Abstract

The invention relates to the technical field of detection equipment, in particular to an efficient detection device and method for bactericide residues in grains, the device comprises a box body, a base is mounted in the box body, a treatment cabin is fixedly connected to the top of the base, and a crushing assembly which extends into the treatment cabin and is used for crushing the grains is arranged at the bottom of the treatment cabin; a water supply pipe is arranged at the top of the treatment cabin, the water supply pipe communicates with a sprinkler head fixedly connected to the top of the treatment cabin, the other end of the sprinkler head extends into the treatment cabin and is located above the crushing assembly, a spoiler is arranged in the sprinkler head, and a water outlet hole is formed in the center of the spoiler; the side edges of the spoilers are fixedly connected with pull rods, and the other ends of the pull rods are fixedly connected with sliding plates. According to the invention, through the integrated design of a water flow driving mechanical structure and gas purging, the water spraying head can automatically complete residue cleaning after water supply is finished each time, manual intervention is avoided, the sample pretreatment efficiency is improved, and the overall detection efficiency of cereal grains is further improved.

Description

Technical Field

[0001] This invention relates to the field of detection equipment technology, specifically to a highly efficient detection device and method for fungicide residues in grains. Background Technology

[0002] As a vital strategic resource for ensuring national food security and people's livelihood, the scale of grain imports has continued to expand with the progress of global economic integration. However, pesticide residues in imported grains have become a key risk factor affecting the quality and safety of agricultural products and the ecological environment. Succinate dehydrogenase inhibitors (SDHIs), as highly effective pesticides widely used in recent years for the control of crop diseases, have increased crop yields due to their unique mechanism of action and broad spectrum of action. However, long-term and extensive use has also led to a growing risk of residues in grains, posing a serious challenge to the quality and safety supervision of imported grains.

[0003] In existing technologies, research on pesticide residue detection has mostly focused on simple matrices such as fruits and vegetables. However, grain matrices (such as wheat, soybeans, and rice) are rich in complex components such as starch, oil, protein, and natural pigments, which significantly interfere with the extraction, purification, and qualitative and quantitative analysis of target substances during the detection process. Taking a pesticide detector with the product name "Yuntang" YT-NY / BN as an example, it mainly consists of a shell, and a stirring chamber, stirring components, water supply components, and a pretreatment chamber installed inside the shell. During use, the sample is placed in the stirring chamber and crushed and stirred by the stirring components. Then, the water supply components are used to flush the stirred grains into the pretreatment chamber for water-soluble pretreatment. Subsequently, a mass spectrometer and a liquid chromatograph are used to detect the liquid sample in the pretreatment chamber. However, because the crushed grains contain substances such as starch and oil, residues are easily formed on the water supply components. When the grains are pretreated again, these residues will fall off the water supply components under the impact of the water flow and mix with the new test sample, affecting the accuracy of the next test. Therefore, after each crushing of the grains, the user needs to manually clean the water supply components to avoid the residues on the water supply components interfering with the next test.

[0004] In summary, it is necessary to propose a highly efficient detection device and method for fungicide residues in grains to solve the above problems. Summary of the Invention

[0005] To address the aforementioned issues, this invention provides a highly efficient detection device and method for fungicide residues in grains. The device automatically cleans residues after each water supply, avoiding manual intervention, improving sample pretreatment efficiency, and thus enhancing the overall detection efficiency of grains.

[0006] To achieve the above objectives, the technical solution of the present invention is as follows: A high-efficiency detection device for fungicide residues in grains includes a box, a base installed inside the box, a processing chamber fixedly connected to the top of the base, and a crushing component extending into the processing chamber for crushing grains at the bottom of the processing chamber.

[0007] The top of the processing chamber is equipped with a water supply pipe, which is connected to a water spray head fixedly connected to the top of the processing chamber. The other end of the water spray head extends into the processing chamber and is located above the crushing component. The water spray head is equipped with a baffle plate, and the baffle plate has a water outlet at its center. Each side of the baffle plate is fixedly connected to a tie rod, and the other end of each tie rod is fixedly connected to a sliding plate. The end of the water supply pipe away from the water spray head extends out of the chamber and is connected to a water supply component fixedly connected to the outer wall of the chamber for supplying water to the water supply pipe.

[0008] The inner wall of the spray head is provided with a number of chambers corresponding to the slide plates. The side of each chamber that is far apart from each other is connected to a channel extending to the outlet of the spray head. The channel is located inside the spray head pipe wall. The top of each chamber is connected to an air inlet pipe extending to the end of the spray head outside the treatment chamber. Each air inlet pipe is provided with a first one-way valve. The slide plates are slidably fitted into the corresponding chambers. The side of each slide plate near the pull rod is provided with a return spring sleeved on the outside of the pull rod. The other side of the return spring is fixedly connected to the inner wall of the chamber.

[0009] The top of the base is equipped with an air supply assembly for supplying air to the chamber, and the air supply assembly is connected to the air inlet pipe.

[0010] The technical principle of the above solution is as follows: automatic cleaning is achieved through the synergistic effect of the internal mechanical structure of the spray head and gas power. When the water supply component delivers water to the water supply pipe, the water flows into the treatment chamber through the outlet hole in the center of the baffle plate. At this time, the water pressure pushes the baffle plate to one side of the treatment chamber, causing the pull rods and slide plates on both sides to slide along the chamber and compress the return spring, giving the return spring elastic potential energy. During the movement of the slide plate, the space inside the chamber increases and the air pressure decreases, allowing external gas to enter the chamber through the first one-way valve. When the water supply stops, there is no water flow impacting the baffle plate. At this time, the return spring elastically resets and pushes the slide plate to slide in the opposite direction, causing the gas inside the chamber to be compressed and sprayed out from the outlet of the spray head through the channel and the second one-way valve, forming a directional airflow that blows away and cleans the starch, grease, and other residues adhering to the inner wall of the spray head and the outlet. Through the integrated design of the water flow-driven mechanical structure and gas purging, the spray head automatically completes the residue cleaning after each water supply, avoiding the problems of cumbersome operation and low efficiency caused by manual intervention.

[0011] The above approach has the following beneficial effects:

[0012] 1. This solution integrates the residue cleaning function into the spray head structure, eliminating the need for users to manually disassemble or wipe the water supply components. This reduces manual intervention steps, lowers operational complexity and labor intensity, and improves the pre-processing efficiency of grain samples before testing. It is especially suitable for high-frequency testing scenarios such as ports and laboratories.

[0013] 2. This solution, through the built-in automatic purging mechanism of the spray head, can immediately remove the attached starch, oil and other residues after each grain crushing and rinsing process, avoiding matrix interference caused by residue falling off and ensuring the accuracy and reproducibility of the test results.

[0014] 3. This solution adopts a cleaning method that combines mechanical linkage and pneumatic power. There is no need to add additional complex drive components. The function is integrated through simple structures such as return springs and sliding plates, which not only ensures the overall compactness of the device, but also improves the structural stability and maintenance convenience during long-term use.

[0015] Further processing revealed that the top of the cabin was hinged with a lid.

[0016] Beneficial effects: The lid can seal the top opening of the processing chamber, preventing external dust and impurities from entering the processing chamber and contaminating the samples or interfering with the testing environment. At the same time, it protects the internal components from external damage when the equipment is not in use, improving the safety of the device and the cleanliness of the testing environment.

[0017] Furthermore, a drain pipe is connected to the bottom of the treatment chamber, and the other end of the drain pipe is connected to a collection chamber located on the bottom wall of the tank. A solenoid valve located above the collection chamber is connected to the drain pipe.

[0018] Beneficial effects: The solenoid valve can automatically control the opening and closing of the drain pipe, realizing the timed or quantitative discharge of liquid in the treatment chamber, which can be collected in the collection chamber for subsequent testing.

[0019] Furthermore, the crushing assembly includes a motor fixedly connected to the base, a drive shaft fixedly connected to the motor output shaft, and the other end of the drive shaft extending into the processing chamber and fixedly connected to several crushing blades.

[0020] Beneficial effects: The motor drives the crushing paddles to rotate at high speed through the transmission shaft. The shearing and impact action of the paddles is used to quickly crush the grain samples, ensuring that the sample particles are uniform and the crushing efficiency is high. This provides a sufficient sample pretreatment basis for subsequent pesticide residue extraction and improves the accuracy of the test results.

[0021] Furthermore, the water supply assembly includes a pressure boosting valve connected to a water supply pipe, and the pressure boosting valve is connected to a water supply bottle.

[0022] Beneficial effects: The pressure booster valve can adjust the water flow pressure entering the water supply pipe, ensuring stable water pressure from the spray head and avoiding the impact of water pressure fluctuations on sample rinsing; the water supply bottle, as an independent water storage unit, facilitates quick replacement or replenishment of cleaning water, improving the adaptability and ease of operation of the device in different testing scenarios.

[0023] Furthermore, the gas supply assembly includes a gas supply cylinder, which is connected to a gas delivery pipe, and the gas delivery pipe is connected to an air inlet pipe.

[0024] Beneficial effects: The gas cylinder provides a stable gas supply reserve, and the gas is delivered to the channel through the gas pipeline, ensuring that the high-pressure flushing nozzle has continuous and sufficient gas power when cleaning residues, enhancing the airflow purging effect. At the same time, the independent gas cylinder design avoids dependence on external gas sources, improving the portability and flexibility of the device.

[0025] Furthermore, a controller is installed on the bottom wall of the box, and the controller is electrically connected to the solenoid valve and the motor.

[0026] Beneficial effects: The controller automates key operations such as switching solenoid valves and starting and stopping motors through electrical connection, reducing manual intervention steps and improving pre-processing efficiency before testing. At the same time, it can preset program parameters according to the testing process to achieve the continuous execution of processes such as sample crushing, rinsing, and drainage, significantly improving testing efficiency.

[0027] Furthermore, the inner diameter of the end of the channel near the nozzle outlet is smaller than the inner diameter of the end that connects to the chamber.

[0028] Beneficial effects: The inner diameter of the channel gradually narrows from the chamber end to the outlet end. According to the principles of fluid mechanics, the flow velocity of the gas increases when it flows through the narrow section, forming a high-speed airflow that is ejected from the outlet. This enhances the purging force on the inner wall and edges of the nozzle, ensuring a more thorough cleaning and effectively avoiding detection interference caused by residue adhesion.

[0029] Furthermore, a second check valve is installed at the end of the channel near the nozzle outlet.

[0030] Beneficial effects: The second one-way valve can prevent liquid or sample debris in the processing chamber from flowing back into the gas supply components through the channel, avoiding contamination of the gas supply cylinder or gas pipeline, ensuring the cleanliness and stability of the gas supply system, while extending the service life of key components of the equipment and reducing maintenance costs.

[0031] Furthermore, a highly efficient method for detecting fungicide residues in grains includes the following steps:

[0032] Step 1, Selection of Grains: Select the grains to be tested, perform preliminary processing, and remove impurities from the surface of the grains;

[0033] Step 2, pretreatment of grain: The grain to be tested is placed in the processing chamber, and a solution is added through the water spray head. The motor is turned on so that the crushing blades crush the grain. The water solution is added through the water spray head to flush the crushed grain residue into the collection chamber for subsequent sampling.

[0034] Step 3, sample processing: The crushed grain solution is extracted using liquid-liquid extraction and salting-out methods, and then the sample solution is mixed, stirred and centrifuged using dispersion solid-phase extraction.

[0035] Step 4, Sample detection: The sample solution is detected using liquid chromatography and mass spectrometry to determine the residual amount of succinate dehydrogenase inhibitor bactericide in the sample solution.

[0036] The beneficial effects of the basic scheme are: after water is supplied through the spray nozzles, the chamber automatically blows air to clean the spray nozzles, reducing the need for manual cleaning of the spray nozzles and processing chamber, reducing the number of grain pre-processing steps, thereby reducing the time required for grain pre-processing and improving the overall testing efficiency. Attached Figure Description

[0037] Figure 1 This is an isometric view of the housing of an embodiment of the high-efficiency detection device for fungicide residues in grains of the present invention;

[0038] Figure 2 This is a front sectional view of an embodiment of the high-efficiency detection device for fungicide residues in grains according to the present invention;

[0039] Figure 3 This is a cross-sectional view of the nozzle of an embodiment of the high-efficiency detection device for fungicide residues in grains of the present invention;

[0040] Figure 4 This is an isometric view of the nozzle of an embodiment of the high-efficiency detection device for fungicide residues in grains according to the present invention;

[0041] Figure 5 This is a schematic diagram of the efficient detection method for fungicide residues in grains according to the present invention.

[0042] The reference numerals in the accompanying drawings of the instruction manual include: 1. Housing; 2. Base; 3. Processing chamber; 4. Water supply pipe; 5. Spray head; 6. Baffle plate; 7. Water outlet; 8. Pull rod; 9. Slide plate; 10. Chamber; 11. Channel; 12. Air inlet pipe; 13. First one-way valve; 14. Return spring; 15. Housing cover; 16. Drain pipe; 17. Collection chamber; 18. Solenoid valve; 19. Motor; 20. Drive shaft; 21. Crushing blade; 22. Pressure booster valve; 23. Water supply bottle; 24. Air supply bottle; 25. Air delivery pipe; 26. Controller; 27. Second one-way valve. Detailed Implementation

[0043] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0044] In the description of this invention, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing the invention and for simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0045] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this invention based on the specific circumstances.

[0046] The following detailed description illustrates the specific implementation methods:

[0047] Example 1:

[0048] A highly efficient detection device for fungicide residues in grains, as shown in the attached document. Figure 1 and attached Figure 2As shown, the device includes a housing 1, inside which a base 2 is installed. A processing chamber 3 is fixedly connected to the top of the base 2 by screws. A cover 15 is hinged to the top of the processing chamber 3. The cover 15 opens and closes the top opening of the processing chamber 3 via a hinged hinge, which can seal the internal environment of the processing chamber 3 during the testing process to prevent external contaminants from entering the processing chamber 3 and affecting the testing accuracy. At the same time, it protects the internal components from external damage when the equipment is not in use. Furthermore, a water supply pipe 4 is provided on the top of the processing chamber 3, and the water supply pipe 4 is connected to... There is a water nozzle 5 that is snapped onto the cover 15 and extends into the processing chamber 3. The end of the water supply pipe 4 away from the water nozzle 5 extends to the outside of the box 1 and is connected to a water supply component that is snapped onto the outer wall of the box 1 to supply water to the water supply pipe 4. The water supply component includes a pressure boosting valve 22, which is connected to a water supply bottle 23. The pressure boosting valve 22 can adjust the water flow pressure entering the water supply pipe 4 to ensure that the water flow sprayed from the water nozzle 5 has sufficient kinetic energy to rinse the crushed grain sample. At the same time, it drives the baffle plate 6 to move to achieve an automatic cleaning function.

[0049] As attached Figure 3 and attached Figure 4 As shown, a baffle plate 6 is slidably fitted inside the spray head 5, and a water outlet 7 is opened at the center of the baffle plate 6; a pull rod 8 is welded to each side of the baffle plate 6, and a sliding plate 9 is fixedly connected to the other end of each pull rod 8; see attached reference. Figure 2 Appendix Figure 3 and attached Figure 4 It is known that the inner wall of the spray head 5 has a number of chambers 10, the number and position of which correspond to the slide plate 9. The side of each chamber 10 that is far apart from each other is connected to a channel 11 extending to the outlet of the spray head 5. The channel 11 is located inside the pipe wall of the spray head 5. The top of each chamber 10 is connected to an air inlet pipe 12 extending to the end of the spray head 5 located outside the treatment chamber 3. A first one-way valve 13 is installed in each air inlet pipe 12. The first one-way valve 13 can prevent the gas in the chamber 10 from leaking out through the air inlet pipe 12, and only allow outside air to enter the chamber 10 when under negative pressure.

[0050] Furthermore, the slide plates 9 are slidably fitted into the corresponding chambers 10. Each slide plate 9 has a return spring 14 welded to the outside of the pull rod 8 on the side near the pull rod 8. The other end of the return spring 14 is welded to the bottom wall of the chamber 10. When the water pressure disappears, the return spring 14 elastically resets and can drive the slide plate 9 to slide in the opposite direction, storing energy for the next cleaning process. Each end of the channel 11 near the outlet of the spray head 5 is equipped with a second one-way valve 27. The second one-way valve 27 can prevent the sample liquid or impurities in the treatment chamber 3 from flowing back into the channel 11, ensuring that the gas can only be sprayed out of the channel 11 in one direction to clean the nozzle.

[0051] The top of the base 2 is provided with a gas supply assembly for supplying gas to the chamber 10. The gas supply assembly includes a gas supply cylinder 24 that is fixedly connected to the top of the base 2 by screws. The gas supply cylinder 24 is connected to a gas delivery pipe 25. The air inlet pipe 12 is connected to the gas delivery pipe 25. The gas supply cylinder 24 can continuously supply high-pressure gas to the chamber 10, ensuring that the airflow ejected from the channel 11 has sufficient strength to remove starch, grease and other residues attached to the inner wall and outlet of the spray head 5, and to prevent residues from falling off and contaminating subsequent test samples.

[0052] The bottom of the treatment chamber 3 is connected to a drain pipe 16, and the other end of the drain pipe 16 is connected to a collection chamber 17 that is snapped into the bottom wall of the box 1. The drain pipe 16 is connected to a solenoid valve 18 located above the collection chamber 17. By controlling the opening and closing of the solenoid valve 18, the waste liquid in the treatment chamber 3 can be discharged in a timed and quantitative manner. The waste liquid flows into the collection chamber 17 through the drain pipe 16 for collection, effectively avoiding the cumbersome operation and environmental pollution risk caused by manually dumping waste liquid.

[0053] The bottom of the processing chamber 3 is equipped with a crushing component that extends into the processing chamber 3 for crushing grains. The crushing component is located below the water spray head 5. The crushing component includes a motor 19 that is fixedly connected to the base 2 by screws. The output shaft of the motor 19 is coaxially keyed to a drive shaft 20. The other end of the drive shaft 20 extends into the processing chamber 3 and is welded with several crushing blades 21. The motor 19 drives the crushing blades 21 to rotate at high speed through the drive shaft 20. The shearing and impact action of the blades is used to fully crush the grain sample to release the residual bactericide, which is convenient for subsequent extraction and detection. The bottom wall of the chamber 1 is equipped with a controller 26. The preferred model of the controller 26 is LKSC-B. The controller 26 is electrically connected to the solenoid valve 18 and the motor 19. The controller 26 controls the start, stop and speed of the motor 19 and the opening and closing of the solenoid valve 18 through a preset program to realize the automated linkage of crushing, rinsing and draining processes, thereby improving the detection efficiency.

[0054] The specific implementation process is as follows: When pre-treatment of grains before testing is required, the operator first opens the lid 15 by rotating the hinge at the top of the chamber 1, and places the grain sample to be tested into the processing chamber 3 through the opening at the top of the processing chamber 3. Then, the lid 15 is closed to seal the internal environment of the chamber 1, preventing external dust and impurities from entering the processing chamber 3 and interfering with the testing process, while protecting the internal components from external damage. At this time, the controller 26 on the bottom wall of the chamber 1 starts the crushing component. After the motor 19 at the top of the base 2 is powered on, it drives several crushing blades 21 to rotate at high speed in the processing chamber 3 through the transmission shaft 20. The shearing and impact action of the blades crushes the grain sample into uniform particles, allowing the residual bactericide in the grain to be fully released into the sample matrix, laying the foundation for subsequent extraction and testing.

[0055] During the crushing process, the controller 26 synchronously controls the start of the pressure boosting valve 22. The cleaning water in the water supply bottle 23, pressurized by the pressure boosting valve 22, enters the water supply pipe 4. The water flow is delivered along the water supply pipe 4 to the spray head 5 and sprayed into the processing chamber 3 through the water outlet 7 in the center of the baffle plate 6, rinsing the crushed grain particles and ensuring thorough mixing of the sample and water to form a detection solution. During this process, the water flow impacts the baffle plate 6, causing it to move towards the bottom of the processing chamber 3. The baffle plate 6 then drives the pull rod 8 to simultaneously pull the sliding plate 9 towards the bottom of the corresponding chamber 10. As the sliding plate 9 slides, it compresses the return spring 14, which gains elastic potential energy. At this time, the air pressure in the chamber 10 decreases, creating a negative pressure that opens the first one-way valve 13. Simultaneously, the gas supply bottle 24 injects high-pressure gas into the chamber 10 through the gas delivery pipe 25.

[0056] After the sample rinsing is completed, the pressure booster valve 22 is closed to cut off the water supply. The baffle plate 6 loses water pressure, and the reset spring 14 releases its elastic potential energy, causing the slide plate 9 to slide in the opposite direction. The slide plate 9 compresses the gas in the chamber 10 into the channel 11. The gas is then ejected from the outlet of the spray head 5 through the channel 11, forming a directional airflow that blows away the sticky residues such as starch and grease adhering to the inner wall and edges of the outlet of the spray head 5, ensuring that the residues are removed before the water rinse, thus avoiding their impact on the accuracy of subsequent detection. At this time, the first one-way valve 13 in the air inlet pipe 12 closes due to the increased pressure in the chamber 10 to prevent gas leakage. The second one-way valve 27 at the end of the channel 11 opens under the action of the airflow to ensure that the gas is ejected in one direction. The second one-way valve 27 automatically closes after the airflow stops to prevent the detection liquid in the processing chamber 3 from flowing back into the channel 11 and causing pipeline blockage. Subsequently, the controller 26 opens the solenoid valve 18 on the drain pipe 16, and the test liquid in the treatment chamber 3 flows into the collection chamber 17 through the drain pipe 16 for centralized collection, avoiding the risk of environmental pollution caused by manual dumping of waste liquid.

[0057] For continuous testing, the device can repeat the above process. During each water supply-stop cycle, the mechanical linkage and gas purging work together to automatically clean the residue from the spray nozzle 5. This integrated operation of sample crushing, rinsing, residue cleaning, and waste discharge requires no manual intervention. Throughout the process, the controller 26 automatically controls components such as the motor 19 and solenoid valve 18 via electrical connection. Simultaneously, the combination of mechanical structure and pneumatic power reduces manual operation, improves sample pretreatment efficiency before testing, and thus enhances testing efficiency.

[0058] The following comparative experiment, based on the above embodiments, is designed with the "Yuntang" YT-NY / BN pesticide detector to highlight the superiority of the device provided by the present invention in terms of pretreatment efficiency.

[0059] Experimental Objective

[0060] By comparing with existing technologies (using the "Yuntang" YT-NY / BN pesticide detector as a control), the efficiency of the device of the present invention in the pretreatment process of grain samples is verified, specifically in four dimensions: pretreatment time, residue cleaning effect, detection accuracy, and continuous processing capability.

[0061] Experimental Materials and Methods

[0062] Experimental samples

[0063] Two typical grain samples, wheat and soybean (the matrix is ​​rich in starch and oil, and has a high risk of residual interference), were selected. After being crushed and passed through an 80-mesh sieve, a standard of succinate dehydrogenase inhibitors (SDHIs) (fluopyram, concentration 0.1 mg / kg) was added. The sample amount for each group was 50 g, and the experiment was repeated in 3 parallel experiments.

[0064] Experimental Groups

[0065] Experimental group: The "High-efficiency detection device for fungicide residues in grains" of this invention was used to automatically complete crushing, rinsing, residue cleaning and detection liquid collection.

[0066] Control group: Using the existing "Yuntang" YT-NY / BN pesticide detector, after manual crushing, the water supply component (spray head) needs to be manually disassembled and rinsed with deionized water for 2 minutes before subsequent treatment.

[0067] Detection indicators and methods

[0068] Total pretreatment time: The entire time from sample input to the entry of the detection solution into the collection chamber 17 / test tube (including crushing, rinsing, cleaning, and drainage).

[0069] Residue content: After each pretreatment, the inner wall of the nozzle was rinsed with 5 mL of deionized water. The starch content in the rinsing solution was determined by the anthrone colorimetric method, and the oil content was determined by Soxhlet extraction.

[0070] Detection accuracy: The concentration of fluopyram in the test solution was determined by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS), and the spiked recovery rate and relative standard deviation (RSD) were calculated.

[0071] Continuous processing capability: 10 groups of samples were processed continuously, and the RSD changes of the 1st and 10th groups were recorded.

[0072] Table 1 Experimental Results

[0073] index Experimental group (inventive device) Control group (existing device) Preprocessing time per cycle 8.5 ± 0.3 min 13.2 ± 0.5 min (including manual cleaning) Starch residue in the nozzle 0.13 ± 0.01 mg / dose 0.45 ± 0.05 mg / dose Residual grease in the nozzle 0.11 ± 0.005 mg / dose 0.32 ± 0.04 mg / dose Fluopyram recovery rate 92.3% ± 3.5% 81.5% ± 8.2% RSD (n=3) 2.8% 6.7% RSD after 10 consecutive processing 3.1% (Group 10) 8.6% (Group 10)

[0074] in conclusion

[0075] In terms of pretreatment efficiency, the invented device eliminates the need for manual disassembly and cleaning of water supply components in the prior art by automating the crushing, rinsing, residue cleaning and waste liquid discharge, thus significantly shortening the time required for a single pretreatment.

[0076] The device of this invention achieves high efficiency in grain sample pretreatment through the coordinated design of mechanical structure and automated control, and is suitable for batch detection of fungicide residues in grains with complex matrices.

[0077] Example 2:

[0078] As attached Figure 3 As shown, the difference from Embodiment 1 is that, in order to increase the gas flow rate in the channel 11 and thus increase the gas pressure, the inner diameter of the end of the channel 11 near the water outlet of the spray head 5 is smaller than the inner diameter of the end that communicates with the chamber 10.

[0079] The specific implementation process is as follows: The inner diameter of channel 11 gradually narrows from the end of chamber 10 to the end of the outlet. According to the principle of fluid mechanics, the flow velocity increases when the gas flows through the narrow section, forming a high-speed airflow that is ejected from the outlet, which enhances the purging force on the inner wall and edge residue of the nozzle, ensuring more thorough cleaning and effectively avoiding detection interference caused by residue adhesion.

[0080] Example 3:

[0081] The difference from the above embodiments is that, as shown in the appendix Figure 5 As shown, a highly efficient method for detecting fungicide residues in grains includes the following steps:

[0082] Step 1, Selection of Grains: Select the grains to be tested, perform preliminary processing, and remove impurities from the surface of the grains;

[0083] Step 2, pretreatment of grain: The grain to be tested is placed in the processing chamber 3, and a solution is added through the water spray head 5. The motor 19 is turned on so that the crushing blades 21 crush the grain. An aqueous solution is added through the water spray head 5, and the crushed grain residue is flushed into the collection chamber 17 for subsequent sampling.

[0084] Step 3, sample processing: Take out the crushed grain solution and process it using liquid-liquid extraction. After adding a dehydrating agent, shake and centrifuge. Then, use dispersion solid-phase extraction to mix, dehydrate, stir and centrifuge the sample solution.

[0085] Step 4, Sample detection: The sample solution is detected using liquid chromatography and mass spectrometry to determine the residual amount of succinate dehydrogenase inhibitor bactericide in the sample solution.

[0086] Obviously, the above embodiments are merely illustrative examples for clear explanation and are not intended to limit the implementation. Those skilled in the art will recognize that other variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. However, obvious variations or modifications derived therefrom are still within the scope of protection of this invention.

Claims

1. A high-efficiency detection device for fungicide residues in grains, comprising a housing (1), characterized in that, A base (2) is installed inside the box (1), and a processing chamber (3) is fixedly connected to the top of the base (2). A crushing component for crushing grain is provided at the bottom of the processing chamber (3) and extends into the processing chamber (3). The top of the processing chamber (3) is provided with a water supply pipe (4), which is connected to a water spray head (5) fixedly connected to the top of the processing chamber (3). The other end of the water spray head (5) extends into the processing chamber (3) and is located above the crushing component. The water spray head (5) is provided with a baffle plate (6), and the baffle plate (6) has a water outlet hole (7) at its center. The sides of the baffle plate (6) are fixedly connected with pull rods (8), and the other end of the pull rods (8) is fixedly connected with a sliding plate (9). The end of the water supply pipe (4) away from the water spray head (5) extends to the outside of the box (1) and is connected to a water supply component fixedly connected to the outer wall of the box (1) for supplying water to the water supply pipe (4). The inner wall of the spray head (5) is provided with a number of chambers (10) corresponding to the slide plate (9). The side of the chambers (10) that are far apart from each other is connected to a channel (11) extending to the outlet of the spray head (5). The channel (11) is located inside the pipe wall of the spray head (5). The top of the chambers (10) is connected to an air inlet pipe (12) extending to the end of the spray head (5) located outside the treatment chamber (3). The air inlet pipe (12) is provided with a first one-way valve (13). The slide plate (9) is slidably fitted into the corresponding chambers (10). The side of the slide plate (9) close to the pull rod (8) is provided with a return spring (14) sleeved outside the pull rod (8). The other side of the return spring (14) is fixedly connected to the inner wall of the chamber (10). The base (2) is provided with an air supply assembly for supplying air to the chamber (10) at the top, and the air supply assembly is connected to the air inlet pipe (12).

2. The high-efficiency detection device for fungicide residues in grains according to claim 1, characterized in that, The top of the processing compartment (3) is hinged with a box cover (15).

3. The high-efficiency detection device for fungicide residues in grains according to claim 2, characterized in that, The bottom of the processing chamber (3) is connected to a drain pipe (16), and the other end of the drain pipe (16) is connected to a collection chamber (17) located on the bottom wall of the box (1). A solenoid valve (18) located above the collection chamber (17) is connected to the drain pipe (16).

4. The high-efficiency detection device for fungicide residues in grains according to claim 3, characterized in that, The crushing assembly includes a motor (19) fixedly connected to the base (2), and a drive shaft (20) is fixedly connected to the output shaft of the motor (19) on the same axis. The other end of the drive shaft (20) extends into the processing chamber (3) and is fixedly connected to several crushing blades (21).

5. The high-efficiency detection device for fungicide residues in grains according to claim 4, characterized in that, The water supply assembly includes a booster valve (22) connected to the water supply pipe (4), and the booster valve (22) is connected to a water supply bottle (23).

6. The high-efficiency detection device for fungicide residues in grains according to claim 5, characterized in that, The gas supply assembly includes a gas cylinder (24), which is connected to a gas delivery pipe (25), and the gas delivery pipe (25) is connected to an air inlet pipe (12).

7. The high-efficiency detection device for fungicide residues in grains according to claim 6, characterized in that, The bottom wall of the box (1) is equipped with a controller (26), which is electrically connected to the solenoid valve (18) and the motor (19).

8. The high-efficiency detection device for fungicide residues in grains according to claim 7, characterized in that, The inner diameter of the end of the channel (11) near the nozzle outlet is smaller than the inner diameter of the end that connects to the chamber (10).

9. The high-efficiency detection device for fungicide residues in grains according to claim 8, characterized in that, Each channel (11) is equipped with a second check valve (27) at the end near the nozzle outlet.

10. A highly efficient method for detecting fungicide residues in grains, based on the highly efficient detection device for fungicide residues in grains as described in any one of claims 1-9, characterized in that, Includes the following steps: Step 1, Selection of Grains: Select the grains to be tested, perform preliminary processing, and remove impurities from the surface of the grains; Step 2, pretreatment of grain: The grain to be tested is placed in the processing chamber (3), and a solution is added through the water spray head (5). The motor (19) is turned on so that the crushing blades (21) crush the grain. An aqueous solution is added through the water spray head (5) and the crushed grain residue is flushed into the collection chamber (17) for subsequent sampling. Step 3, sample processing: The crushed grain solution is extracted using liquid-liquid extraction and salting-out methods, and then the sample solution is mixed, stirred and centrifuged using dispersion solid-phase extraction. Step 4, Sample detection: The sample solution is detected using liquid chromatography and mass spectrometry to determine the residual amount of succinate dehydrogenase inhibitor bactericide in the sample solution.

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