Net membrane type mycelium gas mist fermentation tank

Abstract

The application discloses a net membrane type mycelium gas mist fermentation tank, which comprises a tank body, a culture carrier assembly arranged in the tank body and used for culturing strains, a driving assembly used for driving the culture carrier assembly to rotate, a scraper assembly installed in the tank body and matched with the driving assembly to collect mycelium grown on the surface of the culture carrier assembly, and a supply assembly installed in the tank body and used for supplying materials required by strain fermentation; the culture carrier assembly comprises two concentrically sleeved micro-pipe net carriers, connecting pieces are fixedly installed between one end of the two micro-pipe net carriers, a water supply assembly capable of rotating with the micro-pipe net carriers and supplying heat preservation water into the micro-pipe net carriers is installed in the tank body, and the inner and outer sides of the two micro-pipe net carriers are both provided with the scraper assembly. In the application, the micro-pipe net carriers are directly in heat conduction with the strains, the heat conduction is uniform, the heat transfer efficiency is high, and the strains are uniformly fermented. The whole structure of the application is simple and convenient for practical use.

Description

Technical Field

[0001] This invention belongs to the field of bioreactor technology, and particularly relates to a mesh-type mycelium aerosol fermenter. Background Technology

[0002] Fermentation technology is classified into solid-state fermentation and submerged liquid fermentation based on the form of the fermentation substrate. Solid-state fermentation is a fermentation process carried out on a solid culture medium with low moisture content. Liquid fermentation involves the complete immersion of the microbial cells in a liquid fermentation medium to conduct the biological reaction.

[0003] Solid-state fermentation has advantages such as simple equipment, easy operation, strong adaptability, low energy consumption, low emissions of waste, and minimal environmental pollution. However, its substrate consists of solid materials, a small amount of water, and air, resulting in poor fluidity and variations in nutrient, microbial concentration, temperature, and humidity levels. Furthermore, the biological heat can cause rapid water evaporation, leading to slow fermentation or even termination of fermentation. These factors result in uneven or incomplete fermentation, severely impacting the solid-state fermentation process and product quality.

[0004] Liquid fermentation substrates have good fluidity and relatively uniform mass and heat transfer; however, the structure of liquid fermentation bioreactors is relatively complex and energy consumption is high. Liquid deep fermentation generally uses aerated mechanically stirred fermenters. Mechanical stirring has high shear force, which can easily damage mycelium. Aeration at the bottom of the tank can easily lead to mycelium floating cover phenomenon. High sugar and high protein fermentation liquids have a large amount of foam, which can easily escape liquid and contaminate bacteria. Summary of the Invention

[0005] Purpose of the invention: The purpose of this invention is to provide a mesh-type mycelium aerosol fermenter that can perform uniform fermentation and has a simple structure.

[0006] Technical Solution: This invention discloses a mesh-type mycelium aerosol fermentation tank, comprising a tank body, a culture carrier assembly disposed inside the tank body for culturing microorganisms, a drive assembly for driving the culture carrier assembly to rotate, a scraper assembly installed inside the tank body and cooperating with the drive assembly to collect the mycelium growing on the surface of the culture carrier assembly, and a supply assembly installed inside the tank body for providing substances required for microbial fermentation; the culture carrier assembly includes two concentrically nested microtube mesh carriers, with a connector fixedly installed between one end of the two microtube mesh carriers; a water supply assembly installed inside the tank body that can rotate with the microtube mesh carriers and supply heat-insulating water into the microtube mesh carriers; and scraper assemblies are provided on both the inner and outer sides of the two microtube mesh carriers.

[0007] Furthermore, the microtubular mesh carrier is a cylindrical mesh structure made of hollow tubes.

[0008] Furthermore, the connector includes connecting ring plates that are fixedly connected to one end of each of the two microtubular mesh carriers, and a support rod that is fixedly connected between the two connecting ring plates.

[0009] Furthermore, the drive assembly includes a first motor mounted on the tank body, a transmission gear fixedly connected to the output shaft of the first motor, and a gear ring fixedly mounted on the external micro-tube mesh carrier and meshing with the transmission gear; two fixing rings are fixedly installed on the outer surface of the external micro-tube mesh carrier, and two arc-shaped protrusions for supporting the fixing rings are provided on the inner wall of the tank body, and ball bearings for reducing friction are installed in the grooves opened on the top of the protrusions.

[0010] Furthermore, the scraper assembly includes a support platform fixedly connected to the flat lid of the tank body, a first blade holder fixedly installed on the support platform, an electric telescopic rod fixedly installed on the first blade holder, a second blade holder fixedly connected to the output end of the electric telescopic rod, a scraper disposed on one side of the second blade holder for harvesting mycelium, and an electric hinge installed between the second blade holder and the scraper for adjusting the relative angle between the two.

[0011] Furthermore, the supply assembly includes a first atomizing tube installed inside the tank and cooperating with the drive assembly to spray culture medium onto the surface of the culture carrier assembly, an inoculation tube for spraying bacterial strains, and a second atomizing tube installed inside the tank for supplying nutrients to the culture medium and providing a humid environment; the first atomizing tube, the inoculation tube, and the second atomizing tube are all fixedly connected to the flat tank cover of the tank, and the inlet ends of the first atomizing tube, the inoculation tube, and the second atomizing tube all extend to the outside of the tank.

[0012] Furthermore, the water supply assembly includes a rotating ring plate fixedly connected to the other end of the microtube mesh carrier and a fixed ring plate fixedly connected to the flat tank cover of the tank body. The rotating ring plate and the fixed ring plate are rotatably connected. Each of the rotating ring plate and the fixed ring plate has a groove at one end, and the grooves of the rotating ring plate and the fixed ring plate are joined together to form a liquid storage cavity for containing liquid. A sealing ring to prevent water leakage is installed on the rotating ring plate. A water passage hole connecting the hollow tube and the liquid storage cavity is opened at the end of the rotating ring plate fixedly connected to the microtube mesh carrier. An inlet pipe and an outlet pipe communicating with the liquid storage cavity and the liquid supply equipment are fixedly installed on the flat tank cover.

[0013] Furthermore, it also includes an insulation sleeve with double-sided insulation layers installed on the outer periphery of the tank, wherein the outer layer of the insulation sleeve is filled with heat insulation cotton, the inner layer is filled with water or steam, and the water inlet and outlet pipes of the inner layer are connected to the external water supply equipment or steam supply equipment.

[0014] Furthermore, it also includes a support frame and a second motor fixedly mounted on the support frame. The tank body is rotatably mounted on the support frame, and the output shaft of the second motor is fixedly connected to the tank body.

[0015] Furthermore, an elliptical cover is installed on one end of the tank near the connecting ring plate, and the elliptical cover is provided with a collection port for collecting the grown mycelium, and a cover plate is installed at the collection port.

[0016] Beneficial Effects: Compared with the prior art, the present invention has the following significant advantages: The micro-tube mesh carrier of the present invention conducts heat directly between itself and the inoculum, resulting in uniform heat conduction and high heat transfer efficiency, which is beneficial for uniform fermentation of the inoculum; furthermore, the micro-tube mesh carrier is designed as a cylindrical mesh structure, which helps improve the fluidity inside the tank. The micro-tube mesh carrier of the present invention is rotatably installed inside the tank, and in conjunction with other components, it not only facilitates uniform fermentation of the inoculum but also facilitates the collection of the grown mycelium. The overall structure of the present invention is simple and convenient for practical use. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is another structural schematic diagram of the present invention; Figure 3 This is a radial sectional view of the tank body of the present invention; Figure 4 This is an axial sectional view of the tank body of the present invention; Figure 5 This is a schematic diagram of the culture carrier assembly and the planar lid of the present invention; Figure 6 This is a schematic diagram of the structure of the two microtubular mesh carriers and the rotating ring plate of the present invention; Figure 7 This is a schematic diagram of the internal microtubular network carrier and planar can lid of the present invention; Figure 8 This is a schematic diagram of the scraper of the present invention. Detailed Implementation

[0018] The technical solution of the present invention will be further described below with reference to the accompanying drawings.

[0019] Example 1

[0020] This invention discloses a mesh-type mycelium aerosol fermentation tank, such as... Figure 1 and Figure 2 As shown, it includes a tank body 1, a culture carrier assembly, a drive assembly, a scraper assembly, a supply assembly, a water supply assembly, an insulation sleeve 21, a support frame 24, and a second motor 25. The tank body 1 is rotatably mounted on the support frame 24, and the second motor 25 is fixedly mounted on the support frame 24. The output shaft of the second motor 25 is fixedly connected to the tank body 1, and the second motor 25 drives the tank body 1 to rotate on the support frame 24.

[0021] like Figure 1 and Figure 2As shown, tank 1 is equipped with an observation window 26 and a sight glass lamp 27 for providing illumination. The observation window 26 and sight glass lamp 27 facilitate subsequent observation of the growth of the microorganisms inside tank 1. Preferably, the microtube mesh carrier 5 is equipped with multiple temperature detectors and humidity detectors (not shown in the figure). The temperature detectors can monitor the temperature inside tank 1 in real time, facilitating subsequent adjustment of the liquid temperature introduced into the insulation sleeve 21 and the culture carrier assembly based on the detected temperature, thereby adjusting the temperature inside tank 1. The humidity detectors can monitor the humidity inside tank 1 in real time, facilitating subsequent adjustment of the amount of sterile water introduced into the second atomizing tube 4 based on the detected humidity, thereby adjusting the humidity inside tank 1. Preferably, tank 1 is equipped with a carbon dioxide detector and a camera (not shown in the figure). The carbon dioxide detector detects the concentration of carbon dioxide inside tank 1, and the carbon dioxide concentration is used to determine the mycelial respiration intensity, thereby determining the maturity of the mycelium, facilitating timely harvesting or ending fermentation and removing the mycelium from the tank. The camera facilitates viewing the growth of mycelium inside tank 1.

[0022] Preferably, an exhaust port with a control valve is installed on the tank body 1; a condensate drain pipe with a control valve is installed on the tank body 1, and the condensate drain pipe is located at the bottom of the tank body 1 to facilitate the discharge of condensate from the tank body 1.

[0023] The tank body 1 has two open ends. A flat lid 28 is fixedly installed at one opening, and an oval lid 23 is installed at the other opening. The oval lid 23 has a collection port for collecting the grown mycelium, and a cover plate is installed at the collection port. When culturing the inoculum, the tank body 1 is in a horizontal position. When collecting the grown mycelium, the second motor 25 is started to drive the tank body 1 to rotate 90° to a vertical position, so that the oval lid 23 is at the bottom of the tank body 1. Then the cover plate is opened to facilitate the collection of mycelium from the collection port. Preferably, two symmetrically arranged levers 30 are rotatably installed on the oval lid 23. The levers 30 are used to clean the mycelium on the inner wall of the oval lid 23. The part of the lever 30 inside the tank body 1 is set with an arc shape matching the inner wall of the oval lid 23. The end of the lever 30 outside the tank body 1 is equipped with a handle to facilitate the user to rotate the lever 30. When collecting mycelium from the collection port, some mycelium will stick to the inner wall of the oval cover 23. By rotating the two levers 30, the levers 30 clean the mycelium on the oval cover 23, so that the sticky mycelium falls to the collection port.

[0024] like Figure 1 , Figure 2 and Figure 4As shown, the insulation jacket 21 is installed on the outer periphery of the tank 1, and the insulation jacket 21 has double-sided insulation layers. The outer layer of the insulation jacket 21 is filled with heat-insulating cotton 22, and the inner layer is filled with water or steam. The inlet and outlet pipes of the inner layer are connected to the external water supply equipment or steam supply equipment. The water circulation pump of the water supply equipment keeps the water in the insulation jacket 21 in a state of circulation. Preferably, the water supply equipment connected to the inner layer is a water supply equipment with heating and cooling functions for practical application. In actual use, if it is necessary to cool the tank 1, cooling water can be introduced into the inner layer through the external water supply equipment to achieve rapid cooling; if it is necessary to insulate the tank 1, insulation water at a preset temperature can be introduced into the inner layer through the external water supply equipment to achieve insulation of the tank 1; if it is necessary to sterilize the tank 1, steam can be introduced into the inner layer through the external steam supply equipment to achieve heating and sterilization of the tank 1.

[0025] like Figures 3 to 7 As shown, the culture carrier assembly includes a microtubular mesh carrier 5 and connectors. The microtubular mesh carrier 5 is a cylindrical mesh structure made of hollow tubes, which are connected to the water supply component. The hollow tubes are made of high-temperature resistant, corrosion-resistant, and biocompatible materials, and are manufactured into a cylindrical mesh structure using 3D printing or weaving processes. Two microtubular mesh carriers 5 are provided, and their diameters are different. The larger-diameter microtubular mesh carrier 5 is fitted around the outer periphery of the other microtubular mesh carrier 5, and the two microtubular mesh carriers 5 are concentrically arranged. The diameters of both microtubular mesh carriers 5 are smaller than the diameter of the tank 1, meaning there is a certain distance between the outer microtubular mesh carrier 5 and the inner wall of the tank 1, which facilitates the growth of the microorganisms. In actual use, the number of microtubular mesh carriers 5 can be set according to the growth characteristics of the microorganisms and space requirements. The connector includes a connecting ring plate 6 and a support rod 7. Two connecting ring plates 6 are provided, and their diameters are adapted to the two micro-tube mesh carriers 5. The two connecting ring plates 6 are fixedly connected to the ends of the two micro-tube mesh carriers 5 near the elliptical cover 23, respectively. The support rod 7 is fixedly connected between the two connecting ring plates 6, allowing the two micro-tube mesh carriers 5 to form a single unit that can rotate synchronously. In actual use, liquid at a preset temperature is introduced into the hollow tube, and the inoculum is planted on the micro-tube mesh carrier 5, allowing direct and uniform heat conduction between the micro-tube mesh carrier 5 and the inoculum, effectively improving heat transfer efficiency. Furthermore, the micro-tube mesh carrier 5 is cylindrical, allowing mycelium to grow on both its inner and outer sides, which helps increase the amount of inoculum cultured in a single tank 1. The micro-tube mesh carrier 5 is also designed with a mesh structure, which on the one hand improves the internal flow of the tank 1, and on the other hand facilitates the operation of the first atomizing tube 2, the inoculation tube 3, and the second atomizing tube 4.

[0026] like Figure 4 , Figure 6 and Figure 7 As shown, the water supply assembly includes a rotating ring plate 17, a fixed ring plate 18, an inlet pipe 19, and an outlet pipe 20. The number of rotating ring plates 17 and fixed ring plates 18 is the same as the number of micro-tube mesh carriers 5. The rotating ring plate 17 is fixedly connected to the other end of the micro-tube mesh carrier 5, and the fixed ring plate 18 is fixedly connected to the flat tank cover 28. The rotating ring plate 17 and the fixed ring plate 18 are rotatably connected. Grooves are provided at opposite ends of the rotating ring plate 17 and the fixed ring plate 18. The grooves of the ring plate 18 are joined to form a liquid storage chamber 29 for containing liquid. A sealing ring to prevent water leakage is installed on the rotating ring plate 17. That is, the rotating ring plate 17 and the fixed ring plate 18 together form a liquid storage ring structure, and the two sets of liquid storage structures are concentrically arranged. One end of the rotating ring plate 17, which is fixedly connected to the microtube mesh carrier 5, has a water passage hole that connects the hollow tube and the liquid storage chamber 29. The flat tank cover 28 is fixedly installed with an inlet pipe 19 and an outlet pipe 20 that connect to the liquid storage chamber 29 and the liquid supply equipment. In actual application, the water circulation pump of the liquid supply equipment is turned on to keep the water in the microtube mesh carrier 5 in a state of circulation. Preferably, the liquid supply equipment adopts a water supply equipment with heating and cooling functions for convenient practical application.

[0027] like Figure 1 , Figure 2 , Figure 5 and Figure 6 As shown, the driving assembly includes a first motor 8, a transmission gear (not shown in the figure), and a gear ring 9. The first motor 8 is fixedly mounted on the tank body 1, the transmission gear is fixedly connected to the output shaft of the first motor 8, and the gear ring 9 is fixedly mounted on the external microtubular mesh carrier 5, with the transmission gear meshing with the gear ring 9. The first motor 8 drives the transmission gear to rotate, which in turn drives the gear to rotate, thereby driving the culture carrier assembly. Two fixing rings 10 are fixedly mounted on the outer surface of the external microtubular mesh carrier 5. The inner wall of the tank body 1 is provided with two arc-shaped protrusions 11 for supporting the fixing rings 10. The grooves on the top of the protrusions 11 are fitted with balls to reduce friction, which helps to reduce the frictional resistance when the fixing rings 11 rotate relative to the protrusions 11. The fixing rings 11 are fixedly mounted on the outer periphery of the microtubular mesh carrier 5, which on the one hand improves the overall structural stability of the microtubular mesh carrier 5, and on the other hand helps to reduce the damage to the microtubular mesh carrier 5 caused by the protrusions 11 when supporting it.

[0028] like Figures 3 to 8 As shown, the scraper assembly includes a support platform 12, a first blade holder 13, an electric telescopic rod 14, a second blade holder 15, a scraper 16, and an electric hinge. The support platform 12 is fixedly connected to the flat can lid 28. Figure 7As shown, two support platforms 12 are provided, concentrically arranged, and do not interfere with each other, rotating ring plate 17, or fixed ring plate 18. One end of the first blade holder 13 is fixedly connected to the support platform 12. The fixed end of the electric telescopic rod 14 is fixedly installed on the first blade holder 13, and the second blade holder 15 is fixedly connected to the output end of the electric telescopic rod 14. A hydraulic lifting frame is installed between the scraper 16 and the second blade holder 15. The electric telescopic rod 14 is used to adjust the distance between the scraper 16 and the micro-tube mesh carrier 5. The electric hinge is used to adjust the relative angle between the scraper 16 and the second blade holder 15, thereby adjusting the angle of the scraper 16 when harvesting mycelium. The electric hinge is an existing mature device (such as the electric hinge of Yingzhihuang). The electric hinge includes two brackets and a hydraulic rod. One end of the two brackets is rotatably connected, and the two brackets are respectively fixedly installed on the scraper 16 and the second blade holder 15. The hydraulic rod is rotatably installed between the two brackets. When the hydraulic rod extends or retracts, the two brackets rotate relative to each other, thereby adjusting the angle between the scraper 16 and the second blade holder 15. In practical applications, the material of the scraper 16 can be selected according to the characteristics of different mycelia. Preferably, a pressure sensor is installed on the scraper 16 to detect the pressure between it and the mycelia. Scraper assemblies are provided on both the inner and outer sides of the two microtubular mesh carriers 5, so that the scraper 16 can scrape the mycelia on the inner and outer surfaces of the microtubular mesh carriers 5. The first blade holder 13 of the scraper assembly located on the inner side of the inner microtubular mesh carrier 5 is fixedly connected to the flat can lid 28. When collecting the grown mycelium, the second motor 25 is first started to drive the tank 1 to rotate to a vertical position. Then the first motor 8 is started. After the first motor 8 drives the micro-tube mesh carrier 5 to rotate at a constant speed, the distance between the scraper 16 and the micro-tube mesh carrier 5 is adjusted by the electric telescopic rod 14 so that the scraper 16 contacts the mycelium. The electric hinge makes the scraper 16 rotate relative to the first blade holder 13, thereby adjusting the angle at which the scraper 16 collects the mycelium. As the micro-tube mesh carrier 5 rotates, the scraper 16 scrapes the mycelium on the micro-tube mesh carrier 5. The mycelium falls and collects at the oval cover 23. The oval cover 23 is opened to facilitate the collection of mycelium from the collection port.

[0029] The supply component is used to supply the substances required for microbial fermentation into the interior of tank 1, such as... Figures 2 to 4As shown, the supply assembly includes a first atomizing tube 2, an inoculation tube 3, and a second atomizing tube 4. The first atomizing tube 2 is located inside the tank 1 and cooperates with the drive assembly to uniformly spray culture medium onto the surface of the culture carrier assembly. The first atomizing tube 2 is fixedly connected to the flat tank cover 28 of the tank 1, and the inlet end of the first atomizing tube 2 extends to the outside of the tank 1. The first atomizing tube 2 is located between two micro-tube mesh carriers 5, and nozzles are installed on the first atomizing tube 2, each facing one of the two micro-tube mesh carriers 5. The number of nozzles is set according to actual needs, and the number of first atomizing tubes 2 can also be set according to actual needs. In actual use, the inlet end of the first atomizing tube 2 is connected to an external culture medium supply device. The first motor 8 is turned on, driving the two micro-tube mesh carriers 5 to rotate at a constant speed. Then, the delivery pump of the culture medium supply device is turned on, outputting the culture medium to the first atomizing tube 2. The first atomizing tube 2 and the nozzle spray the culture medium onto the micro-tube mesh carrier 5. The micro-tube mesh carrier 5 rotates at a constant speed, ensuring the culture medium is evenly sprayed onto it. Furthermore, the micro-tube mesh carrier 5 is mesh-like, allowing the culture medium to pass through the mesh and fall onto the other side of the micro-tube mesh carrier 5. Preferably, the first atomizing tube 2 is a pulse atomizing tube.

[0030] like Figures 2 to 4 As shown, the inoculation tube 3 is located inside the tank 1 and cooperates with the drive assembly to uniformly spray the bacterial culture onto the surface of the culture carrier assembly. The inoculation tube 3 is fixedly connected to the flat tank cover 28 of the tank 1, and the inlet end of the inoculation tube 3 extends to the outside of the tank 1. The inoculation tube 3 is located between two micro-tube mesh carriers 5, and the inoculation tube 3 is equipped with atomizing nozzles facing the two micro-tube mesh carriers 5 respectively. The number of atomizing nozzles is set according to actual needs, and the number of inoculation tubes 3 can also be set according to actual needs. In actual use, the inlet end of the inoculation tube 3 is connected to the external bacterial culture supply equipment. The first motor 8 is turned on, and the first motor 8 drives the two micro-tube mesh carriers 5 to rotate at a uniform speed. Then, the delivery pump of the bacterial culture supply equipment is turned on, and the bacterial culture is output to the inoculation tube 3. The inoculation tube 3 and the atomizing nozzles spray the culture medium onto the micro-tube mesh carrier 5. The micro-tube mesh carrier 5 rotates at a uniform speed, and the bacterial culture is evenly sprayed onto the micro-tube mesh carrier 5. The micro-tube mesh carrier 5 is mesh-shaped, and the bacterial culture can pass through the mesh and fall onto the other side of the micro-tube mesh carrier 5. When spraying the culture medium and the bacterial strain, the first motor 8 is driven in opposite directions, which is conducive to the uniform distribution of the bacterial strain and the culture medium.

[0031] like Figures 2 to 4As shown, the second atomizing tube 4 is located inside the tank 1 and is used to supply nutrients, provide a humid environment, and increase dissolved oxygen in the tank 1. The second atomizing tube 4 is fixedly connected to the flat tank cover 28 of the tank 1, and the inlet end of the second atomizing tube 4 extends to the outside of the tank 1. The second atomizing tube 4 is located on the central axis of the microtube mesh carrier 5, and atomizing nozzles are installed on the second atomizing tube 4, with the number of nozzles set according to actual needs. In actual use, the inlet end of the second atomizing tube 4 is connected to the external nutrient solution supply equipment, sterile water supply equipment, and sterile air supply equipment through connecting pipes and multi-way valves. The second atomizing tube 4 atomizes the nutrient solution or sterile water into an aerosol form and evenly disperses it inside the tank 1. The nutrient solution or sterile water aerosol coats the surface of the bacteria to provide nutrients or control humidity and oxygenation. When nutrients need to be provided to the microorganisms in tank 1, the delivery pump of the nutrient solution supply equipment is turned on, and the nutrient solution required by the microorganisms is output to the second atomizing pipe 4. The second atomizing pipe 4 and the atomizing nozzle spray the nutrient solution into the tank. When the humidity in tank 1 is lower than the preset value, the delivery pump of the sterile water supply equipment is turned on, and sterile water is output to the second atomizing pipe 4. The second atomizing pipe 4 and the atomizing nozzle atomize the sterile water and spray it into the tank, increasing the humidity inside tank 1. When the dissolved oxygen concentration in tank 1 is lower than the preset value or the carbon dioxide concentration is higher than the preset value, the delivery valve of the sterile air supply equipment is turned on, and sterile air is output to the second atomizing pipe 4. The second atomizing pipe 4 and the atomizing nozzle spray the sterile air into the tank, increasing the dissolved oxygen content inside tank 1 and reducing the carbon dioxide exhaust volume.

[0032] In operation, steam is first introduced into the tank 1 and the inner layer of the insulation jacket 21 through the second atomizing tube 4. Once the temperature inside the tank 1 reaches the preset temperature and is maintained for a certain period, the tank 1 and its internal components are sterilized. After sterilization, sterile gas (in liquid state) is introduced into the fermenter through the second atomizing tube 4 to lower the temperature inside the tank 1 to the required level. Cooling water is then introduced into the insulation jacket 21 and the micro-tube mesh carrier 5 for forced cooling. When the temperature approaches the fermentation temperature, the cooling water supply is stopped, and the heating functions of the external liquid supply equipment and water supply equipment are activated. Once the water reaches the fermentation temperature, the water circulation pumps of the external liquid supply equipment and water supply equipment are restarted to introduce insulating water into the insulation jacket 21 and the micro-tube mesh carrier 5. When the temperature inside tank 1 reaches the fermentation temperature, the first motor 8 is turned on, causing the microtubular network carrier 5 to rotate at a uniform speed. The culture medium is evenly sprayed onto the microtubular network carrier 5 through the first atomizing tube 2. After the culture medium spraying is completed, the first motor 8 is controlled to drive the microtubular network carrier 5 to rotate in the opposite direction. The inoculum is evenly sprayed onto the microtubular network carrier 5 through the inoculation tube 3 for colonization. After colonization is completed, the first motor 8 is kept in reverse rotation, and nutrient solution is sprayed into the tank 1 through the second atomizing tube 4 to provide nutrition for the inoculum. After inoculation, static fermentation is performed. During fermentation, temperature detectors, humidity detectors, carbon dioxide detectors, and cameras monitor the fermentation status inside tank 1. The temperature of the insulation jacket 21 and the circulating water inside the microtubular network carrier 5 are adjusted according to the actual situation, and the culture medium, nutrient solution, and sterile water are replenished. The mycelial growth is judged based on the monitoring data from the carbon dioxide detector and the camera. When the mycelium has grown, the second motor 25 is first started to drive the tank 1 to rotate to a vertical position. Then, the first motor 8 is started. After the first motor 8 drives the microtubular carrier 5 to rotate at a uniform speed, the distance between the scraper 16 and the microtubular carrier 5 is adjusted by the electric telescopic rod 14, and the angle between the scraper 16 and the microtubular carrier 5 is adjusted by the electric hinge, so that the scraper 16 contacts the mycelium. As the microtubular carrier 5 rotates, the scraper 16 scrapes the mycelium on the microtubular carrier 5. The mycelium falls and collects at the oval cover 23. Sterile air is introduced into the tank 1 through the second atomizing tube 4 to maintain positive pressure inside the tank 1. At the same time, the cover is opened to collect the mycelium. After collection, the cover is closed, and the tank 1 is rotated to a horizontal position by the second motor 25. If multiple fermentations are to be carried out subsequently, the microtube mesh carrier 5 is rotated, and the culture medium is simultaneously and evenly sprayed onto the microtube mesh carrier 5 through the first atomizing tube 2. The temperature and humidity inside the tank 1 are adjusted as needed to carry out secondary continuous fermentation. If multiple fermentations are not to be carried out subsequently, the tank 1 and its internal components are cleaned for future use.

Claims

1. A mesh-type mycelium aerosol fermentation tank, characterized in that: The system includes a tank (1), a culture carrier assembly disposed inside the tank (1) for culturing microorganisms, a drive assembly for rotating the culture carrier assembly, a scraper assembly installed inside the tank (1) and cooperating with the drive assembly to collect mycelia growing on the surface of the culture carrier assembly, and a supply assembly installed inside the tank (1) for providing substances required for microbial fermentation; the culture carrier assembly includes two concentrically nested microtubular mesh carriers (5), with a connector fixedly installed between one end of the two microtubular mesh carriers (5); a water supply assembly is installed inside the tank (1) that can rotate with the microtubular mesh carriers (5) and supply heat-insulating water into the microtubular mesh carriers (5); and scraper assemblies are provided on both the inner and outer sides of the two microtubular mesh carriers (5); the microtubular mesh carriers (5) are cylindrical mesh structures made of hollow tubes; the supply assembly... The water assembly includes a rotating ring plate (17) fixedly connected to the other end of the microtube mesh carrier (5) and a fixed ring plate (18) fixedly connected to the flat tank cover (28) of the tank body (1). The rotating ring plate (17) and the fixed ring plate (18) are rotatably connected. The opposite ends of the rotating ring plate (17) and the fixed ring plate (18) are provided with grooves, and the grooves of the rotating ring plate (17) and the fixed ring plate (18) are spliced ​​to form a liquid storage cavity (29) for containing liquid. A sealing ring to prevent water leakage is installed on the rotating ring plate (17). The end of the rotating ring plate (17) fixedly connected to the microtube mesh carrier (5) is provided with a water passage hole that connects the hollow tube and the liquid storage cavity (29). The flat tank cover (28) is fixedly installed with an inlet pipe (19) and an outlet pipe (20) that connect the liquid storage cavity (29) and the liquid supply equipment.

2. The mesh-type mycelium aerosol fermentation tank according to claim 1, characterized in that: The connector includes a connecting ring plate (6) fixedly connected to one end of each of the two microtubular mesh carriers (5), and a support rod (7) fixedly connected between the two connecting ring plates (6).

3. The mesh-type mycelium aerosol fermentation tank according to claim 1, characterized in that: The drive assembly includes a first motor (8) mounted on the tank (1), a transmission gear fixedly connected to the output shaft of the first motor (8), and a gear ring (9) fixedly mounted on the micro-tube mesh carrier (5) located on the outside and meshing with the transmission gear; two fixing rings (10) are fixedly installed on the outer surface of the micro-tube mesh carrier (5) located on the outside, and two arc-shaped protrusions (11) for supporting the fixing rings (10) are provided on the inner wall of the tank (1), and a ball bearing for reducing friction is installed in the groove opened on the top of the protrusion (11).

4. The mesh-type mycelium aerosol fermentation tank according to claim 1, characterized in that: The scraper assembly includes a support platform (12) fixedly connected to the flat tank cover (28) of the tank body (1), a first blade holder (13) fixedly installed on the support platform (12), an electric telescopic rod (14) fixedly installed on the first blade holder (13), a second blade holder (15) fixedly connected to the output end of the electric telescopic rod (14), a scraper (16) set on one side of the second blade holder (15) for harvesting mycelium, and an electric hinge installed between the second blade holder (15) and the scraper (16) for adjusting the relative angle between the two.

5. The mesh-type mycelium aerosol fermentation tank according to claim 1, characterized in that: The supply assembly includes a first atomizing tube (2) installed inside the tank (1) and cooperating with the drive assembly to spray culture medium on the surface of the culture carrier assembly, and an inoculation tube (3) for spraying bacterial strains, and a second atomizing tube (4) installed inside the tank (1) for supplying nutrients to the culture medium and providing a humid environment; the first atomizing tube (2), the inoculation tube (3) and the second atomizing tube (4) are all fixedly connected to the flat tank cover (28) of the tank (1), and the inlet ends of the first atomizing tube (2), the inoculation tube (3) and the second atomizing tube (4) all extend to the outside of the tank (1).

6. The mesh-type mycelium aerosol fermentation tank according to claim 1, characterized in that: It also includes an insulation sleeve (21) with double-sided insulation jacket installed on the outer periphery of the tank body (1), and the outer jacket (21) is filled with heat insulation cotton (22), the inner jacket is filled with water or steam, and the water inlet pipe and water outlet pipe of the inner jacket are connected to the external water supply equipment or steam supply equipment.

7. The mesh-type mycelium aerosol fermentation tank according to claim 1, characterized in that: It also includes a support frame (24) and a second motor (25) fixedly mounted on the support frame (24). The tank (1) is rotatably mounted on the support frame (24), and the output shaft of the second motor (25) is fixedly connected to the tank (1).

8. The mesh-type mycelium aerosol fermentation tank according to claim 1 or 7, characterized in that: The tank (1) is equipped with an elliptical cover (23) at one end near the connecting ring plate (6), and the elliptical cover (23) is provided with a collection port for collecting the grown mycelium, and a cover plate is installed at the collection port.

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