Automatic fermentation liquor supplementing device for glutathione production

Abstract

The invention relates to the field of fermentation equipment, in particular to an automatic fermentation liquor supplementing device for glutathione production, which comprises: a reaction tank, the top of the reaction tank is provided with an air inlet, the top surface of the reaction tank is fixedly provided with a main air bellow and an auxiliary air bellow, and the main air bellow is correspondingly arranged right above the air inlet and is communicated with the inside of the reaction tank; and a sealing plate. According to the invention, the main air bellow directly senses the volume and pressure change of the purified gas caused by microbial metabolism in the gas phase space at the top of the fermentation tank, and the change is converted into the opening and closing action of the material supplementing valve through the lever mechanism. The feeding process is directly linked with the actual metabolic activity of microorganisms in real time, 'on-demand feeding 'is achieved, and hysteresis and blindness existing in traditional timed and quantitative feeding or program-controlled feeding are effectively avoided. Therefore, nutrition supply can be ensured to be always dynamically matched with microbial requirements, and overnutrition or insufficient nutrition is prevented, so that the yield of glutathione and the stability of the fermentation process are remarkably improved.

Description

Technical Field

[0001] This invention relates to the field of fermentation equipment, and more particularly to an automatic feeding device for fermentation broth used in glutathione production. Background Technology

[0002] Glutathione, as an important bioactive peptide, has wide applications in the fields of medicine, food, and cosmetics. Its industrial production mainly relies on microbial fermentation. During fermentation, in order to maintain the optimal growth and metabolic state of microorganisms, nutrients (such as carbon sources, nitrogen sources, precursors, etc.) need to be continuously or intermittently added to the fermenter.

[0003] Traditional feeding methods often involve manual, timed, and quantitative addition or rely on peristaltic pumps and other equipment controlled by timed programs. These methods have significant drawbacks: manual operation is labor-intensive, inconsistent, and cannot respond in real time to the actual metabolic needs of microorganisms in the tank; automated feeding based on timed programs is lagging and cannot make timely and precise adjustments based on the dynamic changes of key parameters such as dissolved oxygen, pH, or metabolites during the fermentation process, which can easily lead to nutrient over- or under-nutrients, affecting the yield and quality of glutathione. Summary of the Invention

[0004] In view of this, the purpose of this invention is to provide an automatic feeding device for fermentation broth in glutathione production, so as to solve the technical problems in the prior art.

[0005] To achieve the above objectives, the present invention provides an automatic feeding device for fermentation broth in glutathione production, comprising: The reaction vessel has an air inlet at the top, and a main air box and an auxiliary air box are fixedly installed on its top surface. The main air box is located directly above the air inlet and communicates with the inside of the reaction vessel. The sealing plates are respectively fixed to the top of the main air box and the auxiliary air box. Each sealing plate has a push rod fixed to its top. A lever is hinged between the two push rods. The middle part of the lever is hinged and supported by a bracket, and the bracket is fixed to the top surface of the reaction vessel. A sliding sleeve and a hinge rod are provided, wherein the sliding sleeve is slidably mounted on the lever and close to the main air box, and the upper end of the hinge rod is hinged to the bottom of the sliding sleeve; The liquid outlet pipe is fixed to the top of the reaction vessel, and its lower end extends into the interior of the reaction vessel. A conical opening is formed on the inner side of the lower end of the liquid outlet pipe, and a through-hole is provided on the side wall of the conical opening. The liquid inlet pipe is fixedly embedded in the through-hole. A pressure rod, the upper end of which is hinged to the end of the hinge rod away from the sliding sleeve, and the lower end of which is fitted to the inner wall of the tapered opening.

[0006] Preferably, both the main air box and the auxiliary air box are corrugated cylindrical metal pipes of the same specifications, and are made of 316L stainless steel corrugated pipe material.

[0007] Preferably, at least one pair of connecting ears are evenly spaced along the circumferential direction on the periphery of the sealing plate, and a guide rod slides through the middle of each connecting ear in the vertical direction, with the bottom end of the guide rod fixed to the top surface of the reaction vessel.

[0008] Preferably, the lower end of the pressure rod is configured as a tapered structure, the outer periphery of the tapered structure is adapted to the inner periphery of the tapered opening, and the contact surface between the two is a sealing mating surface.

[0009] Preferably, a piston plate is fixedly fitted to the upper end of the pressure rod, and the outer peripheral wall of the piston plate is sealed and fitted to the inner peripheral wall of the liquid outlet pipe, and the two form a sliding sealing fit.

[0010] Preferably, the automatic feeding device further includes an adjustment component, the adjustment component comprising: A first waist-shaped groove is formed at both ends of the lever, a second waist-shaped groove is formed in the middle of the lever, and a sliding member is slidably assembled in the second waist-shaped groove; The top ends of the two push rods are respectively hinged and assembled in the corresponding first waist-shaped groove. The first waist-shaped groove and the second waist-shaped groove have the same extension direction and the same specifications. The upper end of the bracket is hinged to both ends of the slider, and the slider is provided with a locking component for locking its position in the second waist-shaped groove.

[0011] Preferably, the upper and lower sides of the slider are integrally formed with sliders, and the upper and lower inner walls of the second waist-shaped groove are respectively provided with sliding grooves adapted to the sliders. The sliders are slidably embedded in the sliding grooves to form a guide sliding fit.

[0012] Preferably, the locking assembly includes a U-shaped connecting block, bolts, and clamping plates; The U-shaped connecting block is fixed to the top of the sliding member, with its opening facing downwards and fastened to both sides of the lever; The bolt is threaded vertically through the top wall of the U-shaped connecting block; The clamping plate is slidably assembled between the inner walls of the U-shaped connecting block, and an I-shaped assembly groove is provided on its top. The bottom end of the bolt is rotatably assembled in the I-shaped assembly groove through a bearing, and the bottom of the clamping plate abuts against the top surface of the lever.

[0013] Preferably, an anti-slip rubber pad is fixedly bonded to the bottom of the clamping plate, the bottom surface of the anti-slip rubber pad is in close contact with the top surface of the lever, and the bottom surface of the anti-slip rubber pad is provided with evenly distributed anti-slip grooves.

[0014] Preferably, it also includes an insulated and sealed box and a liquid storage tank; The heat-insulating and sealing box is fixedly installed on the top of the reaction vessel, and covers the main air box, auxiliary air box, lever and bracket inside; The liquid storage tank is fixed to the top of the insulated and sealed box, and its interior is connected to the liquid inlet pipe through a pipeline; a pressure relief valve is also fixedly installed on one side of the top of the reaction tank, and the pressure relief valve is connected to the interior of the reaction tank.

[0015] The beneficial effects of this invention are as follows: The automatic feeding device for glutathione production fermentation broth of this invention directly senses changes in the net gas volume and pressure at the top of the fermenter due to microbial metabolism via the main air box, and converts these changes into the opening and closing action of the feeding valve through a lever mechanism. This allows the feeding process to be directly and in real-time linked to the actual metabolic activity of the microorganisms, achieving "on-demand feeding" and effectively avoiding the lag and blindness inherent in traditional timed and quantitative feeding or program-controlled feeding. This ensures that the nutrient supply is always dynamically matched to the microbial needs, preventing nutrient over- or under-nutrition, thereby significantly improving the yield of glutathione and the stability of the fermentation process. By setting up an auxiliary air box with internally sealed fixed air as a reference, and linking it to the main air box in the opposite direction via levers, the expansion or contraction of the two air boxes in the same direction caused by fluctuations in ambient temperature or changes in atmospheric pressure is cleverly counteracted. This mechanism ensures that the feeding trigger signal comes only from the actual gas accumulation generated by metabolism inside the fermenter, greatly reducing the risk of false triggering or failure to trigger, and guaranteeing the long-term operational stability and control accuracy of the unit under various environmental conditions. By incorporating an adjustment assembly with a waist-shaped groove and sliding components, users can easily adjust the position of the lever fulcrum, thereby changing the ratio of the power arm to the resistance arm. This allows the trigger sensitivity of the device to be flexibly set according to the metabolic intensity of different microbial strains and fermentation stages, enhancing the device's process adaptability and control flexibility. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in this invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only for this invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is the main elevation view of the present invention; Figure 2 for Figure 1 Enlarged schematic diagram of the structure at point A in the middle; Figure 3 This is a bottom sectional view of the present invention; Figure 4 for Figure 3 Enlarged schematic diagram of the structure at point B; Figure 5 This is a partial structural cross-sectional view of the main bellows, lever, and liquid outlet pipe in this invention. Figure 6 This is a bottom view of a partial structure of the main air box, connecting plate, and guide rod in this invention; Figure 7 This is a partial structural cross-sectional view of the bracket and sliding components in this invention; Figure 8 for Figure 7 Enlarged schematic diagram of the structure at point C; Figure 9 This is a schematic diagram of the lever structure in this invention; Figure 10 This is a front view of the structure of the reaction vessel and sealed box in this invention.

[0018] The diagram is marked as follows: 1. Reaction vessel; 101. Air inlet; 102. Pressure relief valve; 2. Main air box; 3. Auxiliary air box; 4. Sealing plate; 401. Push rod; 402. Guide rod; 5. Lever; 6. Bracket; 601. Sliding component; 602. U-shaped connecting block; 603. Bolt; 604. Clamping plate; 7. Sliding sleeve; 8. Hinge rod; 9. Pressure rod; 901. Piston plate; 10. Liquid outlet pipe; 1001. Conical opening; 1002. Through port; 11. Liquid inlet pipe; 12. Insulated and sealed box; 13. Liquid storage tank. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to specific embodiments.

[0020] It should be noted that, unless otherwise defined, the technical or scientific terms used in this invention should have the ordinary meaning understood by one of ordinary skill in the art to which this invention pertains. The terms "first," "second," and similar terms used in this invention do not indicate any order, quantity, or importance, but are merely used to distinguish different components. Terms such as "comprising" or "including" mean that the element or object preceding the word encompasses the elements or objects listed following the word and their equivalents, without excluding other elements or objects. Terms such as "connected" or "linked" are not limited to physical or mechanical connections, but can include electrical connections, whether direct or indirect. Terms such as "upper," "lower," "left," and "right" are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0021] In a first aspect, the present invention provides an automatic feeding device for fermentation broth in glutathione production, such as... Figure 1-10 As shown, it includes: The reaction vessel 1 has an air inlet 101 on its top. A main air box 2 and an auxiliary air box 3 are fixedly installed on its top surface. The main air box 2 is located directly above the air inlet 101 and is connected to the inside of the reaction vessel 1. It should be noted that by using the main air box 2 and the auxiliary air box 3 in combination, the air pressure inside the vessel can directly act on the inside of the main air box 2. The auxiliary air box 3 contains a fixed amount of air, which serves as a reference for changes in ambient temperature and pressure.

[0022] The sealing plate 4 is fixed to the top of the main air box 2 and the auxiliary air box 3 respectively. The sealing plate 4 is circular. The circular sealing plate 4 is fixed to the top of the two air boxes by welding. Each sealing plate 4 has a push rod 401 fixed to its top. A lever 5 is hinged between the two push rods 401. The middle part of the lever 5 is hinged and supported by a bracket 6, and the bracket 6 is fixed to the top surface of the reaction vessel 1. The sliding sleeve 7 and the hinge rod 8 are slidably mounted on the lever 5 and close to the main air box 2. The upper end of the hinge rod 8 is hinged to the bottom of the sliding sleeve 7. The liquid outlet pipe 10 and the liquid inlet pipe 11 are provided. The liquid outlet pipe 10 is fixed to the top of the reaction vessel 1, and its lower end opening extends into the interior of the reaction vessel 1. A conical opening 1001 is formed on the inner side of the lower end of the liquid outlet pipe 10. A through-hole 1002 is opened on the side wall of the conical opening 1001. The liquid inlet pipe 11 is fixedly embedded in the through-hole 1002. The liquid inlet pipe 11 is used to connect to an external liquid storage tank, and the replenished material enters through it.

[0023] When the glutathione fermentation device is working, microbial metabolism consumes O2 and produces CO2. Combined with factors such as fermentation broth evaporation, this leads to pressure accumulation inside the tank, resulting in a net increase in the gas volume of the gas phase space at the top of reaction tank 1. This gas expansion drives the main air box 2 to extend upwards, pushing the push rod 401 and one end of the lever 5 upwards. Because the lever 5 is hinged and supported by the bracket 6, the other end of the lever 5 moves downwards, compressing the auxiliary air box 3. This movement is transmitted through the lever 5, driving the sliding sleeve 7 and the hinge rod 8, ultimately driving the pressure rod 9 upwards, causing its conical lower end to disengage from the conical opening 1001, opening the feeding channel. The nutrient solution stored in the external storage tank then flows into the outlet pipe 10 through the inlet pipe 11 under the influence of gravity or a slight pressure difference, and is then dripped into the fermentation broth. When the nutrient solution is added, the pressure inside the tank is released, the main air box 2 resets, and as the microorganisms continue to metabolize, the gas volume inside the tank accumulates again. The device repeats the above actions, achieving intermittent automatic feeding. The feeding rate and frequency are directly determined by the metabolic activity of the microorganisms and the gas production rate, realizing on-demand feeding. Furthermore, the core function of the auxiliary air box 3 is to eliminate environmental interference. When the ambient temperature rises or the atmospheric pressure drops, the air inside both the main air box 2 and the auxiliary air box 3 will expand due to heat or increase in volume, causing them to expand in the same direction (both attempting to elongate). Since the push rods 401 of the two air boxes are connected in opposite directions through the lever 5 (when one push rod 401 moves upward, the other moves downward by the lever 5), this unidirectional environmental interference movement cancels each other out on the lever 5, preventing effective displacement of the sliding sleeve 7 and thus avoiding accidental triggering of material replenishment. Only the movement of the main air box 2 caused by fermentation metabolism (with the gas state inside the auxiliary air box 3 remaining unchanged) can effectively drive the lever 5 to move, ensuring the long-term operational stability and control accuracy of the device under various environmental conditions.

[0024] The pressure rod 9 has its upper end hinged to the end of the hinged rod 8 away from the sliding sleeve 7, and its lower end fitted against the inner wall of the conical opening 1001. When the lower end of the pressure rod 9 presses against the conical opening 1001, a seal is formed, blocking the feeding of materials; when the pressure rod 9 is lifted away from the conical opening 1001, the material can flow from the liquid inlet pipe 11 through the port 1002 into the reaction vessel 1.

[0025] In this embodiment, both the main air box 2 and the auxiliary air box 3 are corrugated cylindrical metal pipes of equal specifications, made of 316L stainless steel. Preferably, both the main air box 2 and the auxiliary air box 3 have an integrally formed annular flange at their bottom. The bottom of the annular flange has an annular groove, and a fluororubber sealing ring is installed in the groove. Therefore, the inner cavity of the main air box 2 and the gas phase space at the top of the reaction vessel 1 form a connected whole, and its volume change directly reflects the net gas pressure / volume change caused by microbial respiration inside the vessel. The auxiliary air box 3 is also installed on the top surface of the reaction vessel 1 via a bottom sealing ring, but its lower end is sealed, and it contains a fixed volume of air, serving as an independent reference unit.

[0026] In addition, the tops of the main air box 2 and the auxiliary air box 3 are sealed by welding with sealing plates 4. The welding is done by argon arc welding, and the sealing process must be carried out under standard environmental conditions (temperature 25±2℃, atmospheric pressure 101.325kPa). The fixed air volume inside the auxiliary air box 3 is 80%-90% of its volume to ensure its effectiveness as a reference for changes in environmental temperature and pressure.

[0027] In this embodiment, at least one pair of connecting ears are evenly spaced along the circumferential direction on the peripheral wall of the sealing plate 4. A guide rod 402 slides through the middle of each connecting ear in the vertical direction, and the bottom end of the guide rod 402 is fixed to the top surface of the reaction vessel 1. Preferably, there are four connecting ears, which are evenly spaced along the circumferential direction on the peripheral wall of the sealing plate 4. The guide rod 402 and the hole on the connecting ear form a sliding pair, which guides and stabilizes the lifting and lowering movement of the sealing plate 4.

[0028] In this embodiment, the lower end of the pressure rod 9 is configured as a tapered structure. The outer circumferential contour of the tapered structure matches the inner circumferential contour of the tapered opening 1001, and the contact surface between the two is a sealing mating surface. An annular sealing groove is provided on the outer circumferential wall of the tapered structure at the lower end of the pressure rod 9, and an O-ring is embedded in the groove to ensure that the sealing ring can be fully compressed when the pressure rod 9 is in contact with the tapered opening 1001, thereby achieving a reliable seal and blocking the material supply channel.

[0029] In this embodiment, a piston plate 901 is fixedly fitted onto the upper end of the pressure rod 9. The outer peripheral wall of the piston plate 901 is sealed and fitted to the inner peripheral wall of the liquid outlet pipe 10, and the two form a sliding seal fit. The piston plate 901 ensures the vertical guidance of the movement of the pressure rod 9 and prevents gas or liquid in the fermenter from leaking along the periphery of the pressure rod 9.

[0030] In this embodiment: the automatic feeding device further includes an adjustment component, which includes: The lever 5 has a first waist-shaped groove at both ends, a second waist-shaped groove in the middle of the lever 5, and a sliding member 601 that is slidably assembled in the second waist-shaped groove; The top ends of the two push rods 401 are respectively hinged and assembled in the corresponding first waist-shaped groove. The first waist-shaped groove and the second waist-shaped groove have the same extension direction and the same specifications. The upper end of the bracket 6 is hinged to both ends of the slider 601, and the slider 601 is provided with a locking component for locking its position in the second waist-shaped groove.

[0031] In this embodiment: the upper and lower sides of the slider 601 are integrally formed with sliders, and the upper and lower inner walls of the second waist-shaped groove are respectively provided with sliding grooves adapted to the sliders. The sliders are slidably embedded in the sliding grooves to form a guide sliding fit. The hinge point position between the bracket 6 and the lever 5 can be adjusted by adjusting the adjustment component to change the ratio of the power arm to the resistance arm of the lever 5, thereby adjusting the sensitivity of the device and the feeding trigger threshold.

[0032] In this embodiment: the locking assembly includes a U-shaped connecting block 602, a bolt 603, and a clamping plate 604; U-shaped connecting block 602 is fixed to the top of sliding member 601, with its opening facing downward and fastened to both sides of lever 5; Bolt 603 is threaded vertically through the top wall of U-shaped connecting block 602; The clamping plate 604 is slidably assembled between the inner walls of the U-shaped connecting block 602. An I-shaped mounting groove is provided on its top. The bottom end of the bolt 603 is rotatably assembled into the I-shaped mounting groove via a bearing. The bottom of the clamping plate 604 abuts against the top surface of the lever 5. The fulcrum position can be adjusted within the range of the second waist-shaped groove via the sliding member 601. When the sliding member 601 moves towards the main air box 2, the power arm on the side of the main air box 2 push rod 401 shortens, and the resistance arm on the side of the auxiliary air box 3 lengthens. At this time, the main air box 2 needs to generate a larger displacement (i.e., more gas volume accumulation) to drive the lever 5 to move effectively. The device sensitivity decreases, making it suitable for stages with vigorous metabolism where the frequency of feeding needs to be reduced. Conversely, moving towards the auxiliary air box 3 increases sensitivity. This allows the triggering sensitivity of the device to be flexibly set according to the metabolic intensity of different strains and fermentation stages, enhancing the process adaptability and control flexibility of the device.

[0033] In this embodiment, an anti-slip rubber pad is fixedly bonded to the bottom of the clamping plate 604. The bottom surface of the anti-slip rubber pad is in close contact with the top surface of the lever 5, and the bottom surface of the anti-slip rubber pad has evenly distributed anti-slip grooves. When the bolt 603 is tightened, the clamping plate 604 is pressed downwards, and the anti-slip rubber pad bonded to its bottom presses tightly against the upper surface of the lever 5, generating a huge frictional force, thereby locking the relative position of the sliding member 601 and the lever 5. The anti-slip grooves on the bottom surface of the anti-slip rubber pad further enhance the locking effect.

[0034] In this embodiment, it also includes an insulated and sealed box 12 and a liquid storage tank 13; The heat-insulating and sealing box 12 is fixedly installed on the top of the reaction vessel 1, and the main air box 2, auxiliary air box 3, lever 5 and bracket 6 are covered inside; The storage tank 13 is fixed to the top of the insulated and sealed box 12, and its interior is connected to the inlet pipe 11 via a pipeline. A pressure relief valve 102 is also fixedly installed on one side of the top of the reaction tank 1, and the pressure relief valve 102 is connected to the interior of the reaction tank 1. The pressure relief valve 102 is a gravity-type pressure relief valve, and its opening pressure is set higher than the trigger pressure of the main air box 2 and the feeding control. After feeding is initiated, microbial metabolism does not stop, and gas continues to be generated inside the tank. Therefore, while feeding is in progress, the pressure inside the tank continues to rise. When the pressure rises to a preset higher safety threshold, the valve core of the pressure relief valve 102 is pushed open for rapid pressure relief. The opening of the pressure relief valve 102 releases the high-pressure gas inside the tank and the main air box 2, causing the pressure to drop rapidly. Once the pressure drops to the set value, the pressure relief valve 102 automatically closes. As the pressure falls, the main air box 2 contracts, and the active force acting on the lever system disappears. Under the contraction force of the main air box 2 and the weight of each component, the pressure rod 9 automatically falls back down, resealing the conical opening 1001, and material feeding stops. The system fully returns to its initial closed and balanced state, waiting for the next pressure accumulation to begin the next cycle, achieving intermittent adaptive material feeding.

[0035] Those skilled in the art should understand that the discussion of any of the above embodiments is merely exemplary and is not intended to imply that the scope of the invention (including the claims) is limited to these examples; within the framework of the invention, the technical features of the above embodiments or different embodiments can also be combined, the steps can be implemented in any order, and there are many other variations of the different aspects of the invention as described above, which are not provided in the details for the sake of brevity.

[0036] This invention is intended to cover all such substitutions, modifications, and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this invention should be included within the scope of protection of this invention.

Claims

1. An automatic feeding device for fermentation broth in glutathione production, characterized in that, The utility model relates to a kind of automatic material supplementing device, including: Reaction tank (1), the top of the reaction tank (1) is equipped with gas inlet (101), its top surface is fixedly installed with main air box (2) and auxiliary air box (3), and the main air box (2) is correspondingly arranged in the just above gas inlet (101) and is communicated with reaction tank (1) inside; Sealing plate (4), two sealing plate (4) are respectively fixed in the top of main air box (2) and auxiliary air box (3), and the top of each sealing plate (4) is fixed with push rod (401), hinged mounting lever (5) is hinged between two push rod (401), the middle part of lever (5) is hinged support by support (6), and the support (6) is fixed on the top surface of reaction tank (1); Slip sleeve (7) and hinged rod (8), the slip sleeve (7) is slidably assembled on the lever (5) and is close to the side of main air box (2), and the upper end of hinged rod (8) is hinged with the bottom of slip sleeve (7); Liquid outlet pipe (10) and liquid inlet pipe (11), the liquid outlet pipe (10) is fixed on the top of reaction tank (1), and its lower end opening extends to the inside of reaction tank (1), the inner side of the lower end of liquid outlet pipe (10) forms taper mouth (1001), the side wall of taper mouth (1001) is equipped with through hole (1002), and the liquid inlet pipe (11) is fixedly embedded in the through hole (1002); Pressing rod (9), the upper end of pressing rod (9) is hinged with the end of hinged rod (8) away from slip sleeve (7), and the lower end is adapted to the inner wall of taper mouth (1001).

2. The automatic feed device for a glutathione production fermentation broth according to claim 1, characterized in that, The main air box (2) and auxiliary air box (3) are equal specification corrugated cylindrical metal pipes, and are made of 316L stainless steel corrugated pipe material.

3. The automatic feed device for a glutathione production fermentation broth according to claim 2, characterized in that, The circumferential wall of the sealing plate (4) is uniformly and spacedly provided with at least one pair of connecting ears, a guide rod (402) is slidably penetrated in the middle of each connecting ear in the vertical direction, and the bottom end of the guide rod (402) is fixed to the top surface of the reaction tank (1).

4. The automatic feed device for a glutathione production fermentation broth according to claim 1, characterized in that, The lower end of the pressing rod (9) is provided in a tapered structure, the outer peripheral contour of the tapered structure is adapted to the inner peripheral contour of the taper mouth (1001), and the two fitting surfaces are sealing fitting surfaces.

5. The automatic feed device for a glutathione production fermentation broth according to claim 1, characterized in that, The upper end of the pressing rod (9) is fixedly sleeved with a piston plate (901), the outer peripheral wall of the piston plate (901) is sealingly fitted with the inner peripheral wall of the liquid outlet pipe (10), and the two constitute a sliding sealing fit.

6. The automatic feed device for a glutathione production fermentation broth according to claim 1, characterized in that, The automatic material supplementing device further comprises an adjusting assembly, the adjusting assembly comprising: A first waist-shaped groove is opened at both ends of the lever (5), a second waist-shaped groove is opened at the middle of the lever (5), and a sliding member (601) is slidably assembled in the second waist-shaped groove; The top ends of the two push rods (401) are respectively hingedly assembled in the corresponding first waist-shaped grooves, and the first waist-shaped grooves and the second waist-shaped grooves have the same extension direction and specifications; The upper end of the support (6) is hinged with both ends of the sliding member (601), and the sliding member (601) is provided with a locking assembly for locking its position in the second waist-shaped groove.

7. The automatic feed device for a glutathione production fermentation broth according to claim 6, characterized in that, The sliding member (601) is integrally formed with sliding blocks on the upper and lower sides, and the upper and lower inner walls of the second waist-shaped groove are correspondingly provided with sliding grooves matched with the sliding blocks, the sliding blocks are slidingly embedded in the sliding grooves, and the guiding sliding fit is formed.

8. The automatic feed device for a glutathione production fermentation broth according to claim 7, characterized by, The locking assembly comprises a U-shaped connecting block (602), a bolt (603) and a clamping plate (604). The U-shaped connecting block (602) is fixed on the top of the sliding member (601), and the opening thereof faces downward and is buckled on both sides of the lever (5). The bolt (603) is threaded through the top wall of the U-shaped connecting block (602) in the vertical direction. The clamping plate (604) is slidingly assembled between the inner side walls of the U-shaped connecting block (602), and a I-shaped assembly groove is formed in the top of the clamping plate (604), the bottom end of the bolt (603) is rotatably assembled in the I-shaped assembly groove through a bearing, and the bottom of the clamping plate (604) is abuttingly matched with the top surface of the lever (5).

9. The automatic feed device for a glutathione production fermentation broth according to claim 8, characterized by, The bottom of the clamping plate (604) is fixedly bonded with an anti-skid rubber pad, the bottom surface of the anti-skid rubber pad is closely abutted with the top surface of the lever (5), and the bottom surface of the anti-skid rubber pad is provided with uniformly distributed anti-skid tooth grooves.

10. The automatic feed device for a glutathione production fermentation broth according to claim 1, characterized in that, It also comprises a heat preservation sealing box (12) and a liquid storage tank (13). The heat preservation sealing box (12) is fixedly covered on the top of the reaction tank (1), and the main air tank (2), the auxiliary air tank (3), the lever (5) and the support (6) are covered inside. The liquid storage tank (13) is fixed on the top of the heat preservation sealing box (12), and the inside thereof is communicated with the liquid inlet pipe (11) through a pipeline; one side of the top of the reaction tank (1) is also fixedly provided with a pressure relief valve (102), and the pressure relief valve (102) is communicated with the inside of the reaction tank (1).

Images