Reusable biocompatible silica gel cell smear preparation device and method

Abstract

The invention relates to the technical field of biomedical detection and pathological slide preparation, and discloses a reusable biocompatible silica gel cell smear preparation device and method.The reusable biocompatible silica gel cell smear preparation device comprises a sealing box, a slide positioning assembly is placed in the sealing box, and a plurality of adhesive glass slides used for cell sedimentation slide preparation are placed on the slide positioning assembly; a plurality of sedimentation assemblies for cell sedimentation are placed on the adhesive glass slide, a box cover is arranged above the sealing box, and a manual negative pressure assembly is arranged above the box cover. A'negative pressure source 'and a'sedimentation device' are separated in space, and a public negative pressure environment is constructed by utilizing a sealing box, so that a user can enjoy the efficiency improvement brought by negative pressure without changing any original operation habit, and the unification of'simplicity and convenience 'and'high efficiency' is really realized. According to the slide positioning assembly, through the mechanical design, one-hand, quick and blind operation fixing and releasing of the adhesive slide are achieved, and the efficiency of batch preparation is greatly improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of biomedical detection and pathology preparation technology, in particular to a reusable biocompatible silica gel cell smear preparation device and method. BACKGROUND

[0002] Cell smear preparation is a key initial step in clinical cytopathology diagnosis and basic medical research, and its quality directly determines the reliability and accuracy of subsequent microscopic observation, morphological analysis and immunocytochemical detection. The traditional cytological preparation method mainly relies on a centrifugal smear machine (cell centrifugal smear machine), which uses centrifugal force to quickly deposit cells in a liquid sample onto a glass slide. Although this method improves the concentration of cell distribution, its inherent defects have become increasingly prominent: strong centrifugal shear force can cause cell deformation, damage or microvillus breakage, especially in fragile cells such as lymphoma cells and active lymphocytes, affecting morphological interpretation; at the same time, the centrifugation process can force cells to form non-physiological two-dimensional false aggregation, interfering with the evaluation of the true cell arrangement structure.

[0003] To overcome the above problems, liquid-based cytology technology has emerged and become the current clinical mainstream. This technology fixes the sample through a preservative solution and removes mucus, blood and other interfering substances, significantly improving smear quality. There are currently two main technical paths on the market: the first is the positive pressure filtration transfer technology represented by ThinPrep, which captures cells through a microporous filter and then transfers the cell layer to a glass slide under positive pressure; the second is the density gradient centrifugation-gravity sedimentation technology represented by SurePath, which purifies cells through gradient centrifugation and then allows them to naturally settle and adhere in a sedimentation cup by gravity.

[0004] However, these existing technical solutions still have their own limitations:

[0005] High technical complexity and cost: both types of technology require specific automated instruments and matching special consumables (such as patented filter membranes, sealed sedimentation cups, and gradient centrifugation tubes), which are expensive to purchase and have high single-detection consumable costs, making it difficult to popularize in primary medical institutions or resource-limited scenarios.

[0006] Potential physical damage: the positive pressure filtration and transfer steps used in ThinPrep technology can still cause non-negligible fluid shear stress on cells. The SurePath technology still cannot completely eliminate the influence of centrifugal force on cells during the pretreatment stage.

[0007] Poor operational flexibility: existing systems are closed and automated processes, making it difficult to meet the needs of laboratories for personalized, small-batch preparation of specific valuable samples (such as trace amounts of cerebrospinal fluid and puncture fluid), and researchers cannot flexibly intervene or optimize preparation parameters.

[0008] Therefore, there is an urgent need in the art for a reusable biocompatible silicone cell smear preparation device and method that can achieve gentle, rapid sedimentation and uniform monolayer distribution of cells without centrifugal force and strong shear force, while being simple to operate, low in cost and not requiring complex special equipment, to fill the technical gap between existing high-end automated systems and original manual drop smear methods, and to meet the needs of a wider range of application scenarios. To this end, we propose a reusable biocompatible silicone cell smear preparation device and method. SUMMARY

[0009] The present application aims to provide a reusable biocompatible silicone cell smear preparation device and method to solve the problems raised in the background art.

[0010] To achieve the above-mentioned purpose, the present application provides the following technical solution: a reusable biocompatible silicone cell smear preparation device and method, comprising a sealed box, a slide positioning assembly is placed in the sealed box, a plurality of adhesive slides for cell sedimentation are placed on the slide positioning assembly, a plurality of sedimentation assemblies for cell sedimentation are placed on the adhesive slides, a box cover is provided above the sealed box, and a manual negative pressure assembly is provided above the box cover.

[0011] Preferably, a placing rack is fixedly connected and arranged in the sealed box, a placing groove is formed in the placing rack, and a clamping ring is fixedly connected and arranged on the outer side of the upper end of the sealed box.

[0012] Preferably, the slide positioning assembly comprises a slide rack, the outer wall of the slide rack is adapted to the placing groove, and a plurality of slide clamping grooves are formed in the slide rack.

[0013] Preferably, a clamping piece is slidingly fitted on the inner wall of one side of the slide clamping groove, the outer end protrusion of the clamping piece abuts against the edge of the adhesive slide, a plurality of springs are fixedly connected and arranged at the inner end of the clamping piece, and the other end of the spring is fixedly connected and arranged with the inner wall of the slide rack.

[0014] Preferably, a triangular block is fixedly connected and arranged at the inner end of the clamping piece, a circular button is slidingly contacted and arranged at the inclined surface of the triangular block, and the circular button is slidingly fitted and arranged through the inner wall of the slide rack.

[0015] Preferably, the adhesive slide is adapted to the inner wall of the slide clamping groove.

[0016] Preferably, the sedimentation assembly comprises a sedimentation ring, the sedimentation ring is made of biocompatible silicone, and a sedimentation cavity is formed in the middle of the sedimentation ring.

[0017] Preferably, plastic clamping strips are connected to the periphery of the box cover, the plastic clamping strips are movably clamped with the clamping ring, and a threaded interface is fixedly and penetratingly connected to one side of the box cover.

[0018] Preferably, the manual negative pressure assembly comprises a negative pressure pipe, a connecting hose is fixedly and penetratingly connected to the bottom end of the negative pressure pipe, the other end of the connecting hose is fixedly and penetratingly connected with a threaded sleeve, the threaded sleeve is threadedly connected with the threaded interface, an exhaust pipe is fixedly and penetratingly connected to one side of the bottom end of the negative pressure pipe, a piston is slidingly matched with the inner wall of the negative pressure pipe, a piston rod is fixedly connected to the piston, the other end of the piston rod is slidingly matched with the inner wall of the negative pressure pipe, a handle is fixedly connected to the outer end of the piston rod, and a grip is fixedly connected to the outer wall of the negative pressure pipe.

[0019] A method for using a reusable biocompatible silicone cell smear preparation device, specifically comprising the following steps:

[0020] S1: placing the sedimentation ring on an adhesive slide to form a liquid-tight sealed sedimentation chamber;

[0021] S2: adding 100-200 muL of cell suspension 105 / mL into the sedimentation chamber;

[0022] S3: standing at room temperature for 15 min to allow the cells to sediment under the action of gravity;

[0023] S4: aspirating the remaining cell suspension in the sedimentation ring, removing the sedimentation ring, drying the adhesive slide at room temperature for 10 min, adding 75% ethanol to fix the cells for 5 min, rinsing with PBS, and then performing subsequent experimental operations.

[0024] For mode two, in step S3, a step of indirectly applying micro-negative pressure to the sedimentation chamber by the manual negative pressure assembly to assist cell sedimentation is further included.

[0025] Compared with the prior art, the beneficial effects of the present application are:

[0026] The present application separates the "negative pressure source" and the "settling device" in space, uses the sealed box to build a common negative pressure environment, and the user does not need to change any original operation habits, so as to enjoy the efficiency brought by the negative pressure, and truly realizes the unity of "simplicity" and "efficiency". The slide positioning assembly realizes the one-handed, rapid and blind operation fixation and release of the adhesive slide by the above mechanical design, and greatly improves the efficiency of batch preparation. More importantly, the mechanical locking force provided by the slide positioning assembly fundamentally guarantees the stability and consistency of the multiple adhesive slides as a whole when taking, moving and even placing in the sealed box to receive negative pressure treatment, avoids cross contamination or operation errors between samples, and is the key support to realize the high-throughput and standardized slide preparation process. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 It is a schematic diagram of the overall structure of a reusable biocompatible silica gel cell smear preparation device and method;

[0028] Figure 2 It is a schematic diagram of the structure of the sealed box of the present application;

[0029] Figure 3 It is a schematic diagram of the structure of the slide positioning assembly and the adhesive slide of the present application;

[0030] Figure 4 It is a schematic diagram of the structure of the slide positioning assembly of the present application;

[0031] Figure 5 It is a schematic diagram of the structure of the adhesive slide and the settling assembly of the present application;

[0032] Figure 6 It is a schematic diagram of the structure of the settling assembly of the present application;

[0033] Figure 7 It is a schematic diagram of the structure of the box cover of the present application;

[0034] Figure 8 It is a schematic diagram of the structure of the manual negative pressure assembly of the present application.

[0035] LEGEND

[0036] In the figure: 1, sealed box; 2, slide positioning assembly; 3, adhesive slide; 4, sedimentation assembly; 5, box cover; 6, manual negative pressure assembly; 101, placing rack; 102, placing groove; 103, clamping ring; 201, slide rack; 202, slide clamping groove; 203, buckle; 204, spring; 205, triangular block; 206, round button; 401, sedimentation ring; 402, sedimentation cavity; 501, plastic clamping strip; 502, threaded interface; 601, negative pressure pipe; 602, connecting hose; 603, threaded sleeve; 604, exhaust pipe; 605, piston; 606, piston rod; 607, handle; 608, grip. DETAILED DESCRIPTION

[0037] The technical solutions in the embodiments of the present application will be described clearly and completely below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the scope of protection of the present application.

[0038] Please refer to Figures 1-8 As shown in the figure, the present application provides a technical solution: a reusable biocompatible silica gel cell smear preparation device and method, which comprises a sealed box 1, a slide positioning assembly 2 is placed in the sealed box 1, a plurality of adhesive slides 3 for cell sedimentation preparation are placed on the slide positioning assembly 2, a plurality of sedimentation assemblies 4 for cell sedimentation are placed on the adhesive slides 3, a box cover 5 is arranged above the sealed box 1, and a manual negative pressure assembly 6 is arranged above the box cover 5.

[0039] Among them, the sealed box 1 and the box cover 5 are made of transparent medical-grade PC material, which can withstand 121℃ high-pressure steam sterilization; the slide positioning assembly 2 is made of medical-grade ABS material, the surface is polished to avoid scratching the adhesive slide 3; the core contact components of the entire device are subjected to sterile treatment to ensure that the cell sample is not contaminated, and are suitable for high-precision scenes such as clinical diagnosis and cell biology experiments.

[0040] Furthermore, by spatially separating the "negative pressure source" and the "sedimentation device," a common negative pressure environment is constructed using the sealed box 1. Users can enjoy the efficiency improvement brought by negative pressure without changing any of their original operating habits, truly achieving a unity of "simplicity" and "efficiency." The slide positioning component 2, through its mechanical design, enables one-handed, rapid, and blind operation for fixing and releasing adhesive slides 3, greatly improving the efficiency of batch preparation. More importantly, the mechanical locking force it provides fundamentally ensures the stability and consistency of multiple adhesive slides 3 when they are handled, moved, or placed in the sealed box 1 for negative pressure treatment, avoiding cross-contamination between samples or operational errors. This is a key support for achieving a high-throughput, standardized slide preparation process.

[0041] In the preferred embodiment of this technical solution, please refer to Figure 2 As shown, a placement rack 101 is fixedly connected inside the sealed box 1, and a placement groove 102 is provided on the placement rack 101. A snap ring 103 is fixedly connected to the outer side of the upper end of the sealed box 1.

[0042] Furthermore, the placement rack 101 and the sealing box 1 are made using an integrated molding process, resulting in high structural strength. The dimensions of the placement groove 102 are precisely matched with the slide holder 201, and the inner wall of the placement groove 102 is equipped with an elastic buffer pad made of medical-grade silicone. This not only enables the rapid positioning and placement of the slide positioning component 2 but also prevents collision damage during placement. The snap ring 103 ensures that the sealing box 1 and the lid 5 do not separate under negative pressure after being closed, meeting the requirements for long-term negative pressure slides.

[0043] In the preferred embodiment of this technical solution, please refer to Figure 4 As shown, the slide positioning assembly 2 includes a slide holder 201, the outer wall of which is adapted to the placement groove 102, and multiple slide slots 202 are provided on the slide holder 201.

[0044] The number of slide slots 202 matches the specifications of the adhesive slides 3, and the spacing between adjacent slide slots 202 is reasonable to avoid mutual interference after the sedimentation components 4 are placed, while providing sufficient operating space for operators to add cell suspension.

[0045] A latching element 203 is slidably fitted on one side of the inner wall of the slide slot 202. The protruding part of the outer end of the latching element 203 abuts against the edge of the adhesive slide 3. Multiple springs 204 are fixedly connected to the inner end of the latching element 203. The other end of the springs 204 is fixedly connected to the inner wall of the slide holder 201.

[0046] The protrusion of the latch 203 ensures stable locking; the spring 204 is a medical-grade stainless steel compression spring to ensure stable locking force of the latch 203 on the glass slide.

[0047] A triangular block 205 is fixedly connected to the inner end of the fastener 203. A circular button 206 is slidably connected to the inclined surface of the triangular block 205. The circular button 206 is slidably engaged with the inner wall of the slide holder 201.

[0048] Furthermore, the contact area between the triangular block 205 and the circular button 206 is polished to ensure that pressing the circular button 206 can quickly drive the latch 203 to retract, thereby enabling the convenient release of the adhesive glass slide 3 and adapting to one-handed blind operation.

[0049] In the preferred embodiment of this technical solution, please refer to Figure 5 As shown, the adhesive slide 3 is adapted to the inner wall of the slide slot 202.

[0050] Furthermore, the adhesive slide 3 is made of ultra-white glass, which facilitates subsequent microscopic observation; its surface is coated with a polylysine adhesive layer, which can effectively prevent cells from falling off during sedimentation, while not affecting cell activity and morphology.

[0051] In the preferred embodiment of this technical solution, please refer to Figure 6 As shown, the sedimentation assembly 4 includes a sedimentation ring 401, which is made of biocompatible silicone, and a sedimentation cavity 402 is provided in the middle of the sedimentation ring 401.

[0052] Furthermore, the settling ring 401 is made of medical-grade liquid silicone, which is non-cytotoxic and non-sensitizing, and does not release harmful substances after contact with the cell suspension. The bottom end of the settling ring 401 can achieve a liquid-tight seal at the contact point with the adhesive glass slide 3 under its own elasticity. The volume of the settling chamber 402 is adapted to the amount of cell suspension added, and the inner wall of the settling chamber 402 is smooth and arc-shaped to avoid cell suspension residue.

[0053] In the preferred embodiment of this technical solution, please refer to Figure 7 As shown, plastic clips 501 are connected around the box cover 5. The plastic clips 501 are movably engaged with the snap ring 103. A threaded interface 502 is fixedly connected through one side of the box cover 5.

[0054] Furthermore, the plastic strip 501 is made of medical-grade PP material, which has good elasticity and toughness and can withstand repeated bending without breaking; an annular sealing groove is set at the contact point between the inner wall of the box cover 5 and the top of the sealed box 1, and a medical silicone sealing ring is embedded in the groove to further improve the overall sealing performance and ensure a stable negative pressure environment.

[0055] In the preferred embodiment of this technical solution, please refer to Figure 8As shown, the manual negative pressure assembly 6 includes a negative pressure pipe 601. A connecting hose 602 is rotatably connected through the bottom end of the negative pressure pipe 601. A threaded sleeve 603 is fixedly connected through the other end of the connecting hose 602. The threaded sleeve 603 is threadedly connected to the threaded interface 502. An exhaust pipe 604 is fixedly connected through one side of the bottom end of the negative pressure pipe 601. A piston 605 is slidably fitted on the inner wall of the negative pressure pipe 601. A piston rod 606 is fixedly connected to the piston 605. The other end of the piston rod 606 is slidably fitted through the inner wall of the negative pressure pipe 601. A handle 607 is fixedly connected to the outer end of the piston rod 606. A grip 608 is fixedly connected to the outer wall of the negative pressure pipe 601.

[0056] Furthermore, both the exhaust pipe 604 and the connecting hose 602 are equipped with one-way valves. The negative pressure pipe 601 can quickly create the required negative pressure environment; the connecting hose 602 is made of medical-grade PVC material, which can be bent at will without affecting airflow, providing ample room for manual operation; the threaded sleeve 603 is easy to tighten and loosen manually; the piston 605 is made of medical-grade silicone material, with excellent sealing performance, ensuring no airflow leakage during the suction process; the piston rod 606 is made of stainless steel with a chrome-plated surface, allowing for smooth sliding, and a sealing sleeve is set at the penetration point with the negative pressure pipe 601 to further improve the sealing performance; the handle 607 and grip 608 are both wrapped in rubber, which is non-slip and wear-resistant, providing high grip comfort and suitable for one-handed suction operation; the one-way valve in the exhaust pipe 604 is an exhaust one-way valve, allowing only the air in the sealed box 1 to be discharged, and the one-way valve in the connecting hose 602 is an intake one-way valve, allowing only outside air to enter the negative pressure pipe 601. The two work together to ensure that the negative pressure environment can be stably created and maintained during manual suction.

[0057] A method for using a reusable biocompatible silicone cell smear preparation device specifically includes the following steps:

[0058] S1: Place the settling ring 401 on the adhesive glass slide 3 to form a liquid-tight settling chamber 402;

[0059] S2: Add 100-200 μL of cell suspension at a concentration of 10⁵ / mL into sedimentation chamber 402;

[0060] S3: Let stand at room temperature for 15 minutes to allow the cells to settle under gravity;

[0061] S4: Aspirate the remaining cell suspension in the sedimentation loop 401, remove the sedimentation loop 401, dry the adhesive slide 3 at room temperature for 10 min, add 75% ethanol to fix the cells for 5 min, rinse with PBS and proceed with subsequent experimental procedures.

[0062] For the second method, step S3 also includes a step of indirectly applying a micro negative pressure to the sedimentation chamber 402 by manually extracting the air from the sealed box 1 through the negative pressure component 6, so as to assist cell sedimentation.

[0063] Working Principle: Device Preparation and Slide Positioning Stage: First, the contact parts of the sealed box 1, slide positioning component 2, settling component 4, box cover 5, and manual negative pressure component 6 are sterilized by high-pressure steam at 121℃ and cooled for later use. Align the slide holder 201 of the slide positioning component 2 with the placement slot 102 of the placement rack 101 inside the sealed box 1 and place it smoothly to achieve positioning. Pick up the adhesive slide 3 with one hand and press the circular button 206 on the slide holder 201. The circular button 206 squeezes the inclined surface of the triangular block 205, pushing the latch 203 to retract and align its edge with the slide slot 202 for insertion. Then release the circular button 206. Under the action of the spring 204, the protrusion of the latch 203 abuts against the edge of the adhesive slide 3, achieving rapid locking of the adhesive slide 3. Repeat the above operation to complete the positioning and fixing of multiple adhesive slides 3, achieving high-throughput preparation.

[0064] Placement of sedimentation component 4 and sample addition stage: Place the sterilized sedimentation ring 401 stably in the center of each adhesive glass slide 3. Utilizing the flexibility and absorbency of the silica gel, a bottom-liquid-tight sedimentation cavity 402 is formed between the two. Then, add approximately 100-200 μL of cell suspension into the sedimentation cavity 402. Incubate at room temperature for 10-15 minutes. During this time, the cells settle naturally under their own gravity. Finally, carefully aspirate the supernatant, remove the sedimentation ring 401, allow the slides to air dry, and proceed with subsequent staining and fixation. This method requires no additional equipment and is extremely simple to operate.

[0065] Cell sedimentation stage: Method 1 Gravity sedimentation: Cover the sealed box 1 containing the positioning slide and sedimentation component with the lid 5, press the plastic clips 501 around the lid 5 so that the plastic clips 501 are tightly engaged with the locking ring 103 of the sealed box 1, thus closing the sealed box 1; place the sealed box 1 at room temperature for 15 minutes, and the cells gradually settle to the surface of the adhesive slide 3 under the action of gravity, thus completing cell adhesion. Method 2: Negative Pressure Assisted Settling: After covering and securing the box cover 5, manually tighten the threaded sleeve 603 of the manual negative pressure component 6 to the threaded interface 502 of the box cover 5, ensuring a seal at the threaded connection. The operator holds the handle 608 of the negative pressure pipe 601 with one hand and the handle 607 of the piston rod 606 with the other, pulling the piston rod 606 upwards. This causes the piston 605 to slide upwards along the inner wall of the negative pressure pipe 601, drawing air from the sealed box 1 into the negative pressure pipe 601 via the connecting hose 602. At this time, the one-way valve in the connecting hose 602 opens, and the exhaust pipe... The exhaust check valve inside 604 is closed; when piston 605 is pulled to the top, press down handle 607 to push piston 605 downward, and the air in negative pressure tube 601 is discharged through exhaust tube 604. At this time, exhaust check valve is opened and air intake check valve is closed; repeat the upward suction operation until the required micro-negative pressure is reached in sealed box 1; maintain the negative pressure environment and place sealed box 1 at room temperature. Micro-negative pressure can accelerate the discharge of gas in cell suspension and promote cell sedimentation on the surface of glass slide. Compared with gravity sedimentation, the sedimentation time can be shortened and the cell distribution is more uniform.

[0066] Subsequent processing and device reset stage: After cell sedimentation, if a negative pressure assisted method is used, first loosen the threaded sleeve 603 to allow outside air to enter the sealed box 1, releasing the negative pressure environment, and then disassemble the manual negative pressure component 6; open the box cover 5, use a pipette to remove the remaining cell suspension in each sedimentation chamber 402 one by one, and then gently pick up the sedimentation ring 401 and remove it from the slide; take the slide holder 201 out of the sealed box 1, press the circular button 206 on the slide holder 201, the circular button 206 squeezes the inclined surface of the triangular block 205, pushes the buckle 203 to retract, releases the lock on the slide, and the adhesive slide 3 can be taken out with one hand; place the removed slide at room temperature to dry for 10 minutes, add 75% ethanol to fix the cells for 5 minutes, rinse with PBS and then perform subsequent staining, microscopic observation and other experimental operations; after the experiment is completed, clean the used device parts, autoclave them again and store them for the next use.

[0067] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0068] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A reusable biocompatible silicone cell smear preparation device comprising a sealed case (1) characterised in that: The sealed box (1) is placed with a slide positioning assembly (2), a plurality of adhesive slides (3) for cell sedimentation are placed on the slide positioning assembly (2), and a plurality of sedimentation assemblies (4) for cell sedimentation are placed on the adhesive slides (3).

2. A reusable biocompatible silicone cell smear preparation device according to claim 1, wherein: The sealed box (1) is fixedly connected with a placing rack (101), the placing rack (101) is provided with a placing groove (102), and the sealed box (1) is fixedly connected with a clamping ring (103) on the outer side of the upper end.

3. A reusable biocompatible silicone cell smear preparation device according to claim 1, wherein: The slide positioning assembly (2) comprises a slide rack (201), the outer wall of the slide rack (201) is matched with the placing groove (102), and a plurality of slide clamping grooves (202) are formed in the slide rack (201).

4. A reusable biocompatible silicone cell smear preparation device according to claim 3, wherein: The inner wall of one side of the slide clamping groove (202) is slidably connected with a clamping buckle (203), the outer end of the clamping buckle (203) is abutted with the edge of the adhesive slide (3), a plurality of springs (204) are fixedly connected to the inner end of the clamping buckle (203), and the other end of the spring (204) is fixedly connected with the inner wall of the slide rack (201).

5. A reusable biocompatible silicone cell smear preparation device according to claim 4, wherein: The inner end of the clamping buckle (203) is fixedly connected with a triangular block (205), the inclined surface of the triangular block (205) is slidably connected with a circular button (206), and the circular button (206) is slidably connected with the inner wall of the slide rack (201).

6. A reusable biocompatible silicone cell smear preparation device according to claim 1, wherein: The adhesive slide (3) is matched with the inner wall of the slide clamping groove (202).

7. A reusable biocompatible silicone cell smear preparation device according to claim 1, wherein: The sedimentation assembly (4) comprises a sedimentation ring (401), the sedimentation ring (401) is made of biocompatible silica gel, and a sedimentation cavity (402) is formed in the middle of the sedimentation ring (401).

8. A reusable biocompatible silicone cell smear preparation device according to claim 1, wherein: The box cover (5) is connected with a plastic clamping strip (501) around, the plastic clamping strip (501) is movably connected with the clamping ring (103), and the box cover (5) is fixedly connected with a threaded interface (502) on one side.

9. A reusable biocompatible silicone cell smear preparation device according to claim 1, wherein: The manual negative pressure assembly (6) comprises a negative pressure pipe (601), a connecting hose (602) is rotatably connected to the bottom end of the negative pressure pipe (601), a threaded sleeve (603) is fixedly connected to the other end of the connecting hose (602), the threaded sleeve (603) is threadedly connected with the threaded interface (502), an exhaust pipe (604) is fixedly connected to one side of the bottom end of the negative pressure pipe (601), a piston (605) is slidably connected to the inner wall of the negative pressure pipe (601), a piston rod (606) is fixedly connected to the piston (605), the other end of the piston rod (606) is slidably connected with the inner wall of the negative pressure pipe (601), a handle (607) is fixedly connected to the outer end of the piston rod (606), and a handle (608) is fixedly connected to the outer wall of the negative pressure pipe (601).

10. A method for using a reusable biocompatible silica gel cell smear preparation device, which comprises the following steps: S1: placing the settling ring (401) on the adhesive slide (3) to form a liquid-tight sealed settling chamber (402); S2: adding 100-200 μL of cell suspension (105 / mL) into the settling chamber (402); S3: standing at room temperature for 15 min to allow the cells to settle under the action of gravity; S4: aspirating the remaining cell suspension in the settling ring (401), removing the settling ring (401), drying the adhesive slide (3) at room temperature for 10 min, adding 75% ethanol to fix the cells for 5 min, rinsing with PBS, and then performing subsequent experimental operations. For mode two, in step S3, a step of indirectly applying a micro-negative pressure to the settling chamber (402) by the manual negative pressure assembly (6) to aspirate the air in the sealed box (1) is further included to assist the cell settling. ​ ​ ​ ​

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