Nucleic acid detection device

Abstract

The invention relates to the medical field, and discloses a nucleic acid detection device which comprises a connecting mechanism, a blocking mechanism and a pushing mechanism, the connecting mechanism comprises a connecting pipe assembly, a sleeve is arranged on the inner wall of the connecting pipe assembly in a penetrating mode, the blocking mechanism is arranged in the connecting mechanism, and the blocking mechanism comprises a swab pipe silica gel plug. A cleavage tube silica gel plug is arranged on one side of the swab tube silica gel plug, the swab tube silica gel plug and the cleavage tube silica gel plug are arranged in the sleeve, and two first blocking parts are arranged in the swab tube silica gel plug. According to the structure, the effects of reducing experiment steps and achieving the same experiment effect are achieved, and the problems that in the prior art, in the nucleic acid detection process, a splitting decomposition tube, a dilution tube and a reaction tube need to be frequently operated, and tube covers need to be frequently opened for adding operation, so that the experiment steps are numerous, the operation difficulty is large, and the operation time is short are solved. The use is very inconvenient; the sample is polluted; and the detection precision is reduced.

Description

Technical Field

[0001] This invention relates to the medical field, and more particularly to a nucleic acid detection device. Background Technology

[0002] With the development of molecular detection technology, nucleic acid detection technology has been widely used. In the context of routine epidemic prevention and control and responses to public health emergencies, the demand for rapid and convenient nucleic acid testing has surged. Traditional devices suffer from problems such as long testing cycles and reliance on specialized facilities. Nucleic acid detection devices have thus emerged to address this need. These devices are primarily used to accurately collect nucleic acid substances from biological samples, achieving rapid identification and analysis of nucleic acids through specific technologies. This provides efficient detection support for pathogen screening and disease diagnosis, facilitating timely prevention and treatment.

[0003] In the existing nucleic acid detection process, lysis tubes, dilution tubes, and reaction tubes are required. The lysis tube is used to hold the lysis buffer and provide a space for mixing the lysis buffer and the sample solution carrying the sample. The dilution tube is used to dilute the sample lysis mixture. The reaction tube is used to hold the reaction solution. The specific process of nucleic acid detection is as follows: Open the cap of the lysis tube → Add the sample solution to the lysis tube containing the lysis buffer, and the sample solution and lysis buffer are mixed to form a sample lysis mixture → The sample is lysed in the lysis tube → Open the cap of the dilution tube → Quantitatively squeeze the sample lysis mixture from the lysis tube into the dilution tube → Mix the sample lysis mixture with the diluent in the dilution tube → Open the cap of the reaction tube → Quantitatively squeeze the diluted sample lysis mixture from the dilution tube into the reaction tube → Mix the diluted sample lysis mixture with the reaction solution in the reaction tube → Sample amplification and fluorescence detection. Because the lysis tubes, dilution tubes, and reaction tubes are set up separately, the nucleic acid detection process requires frequent handling of these tubes, as well as frequent opening of the caps and addition of substances. This results in numerous experimental steps, greater operational difficulty, and extreme inconvenience. In addition, frequent handling of the lysis tubes and reaction tubes, and frequent addition of substances, can easily increase the chance of sample solution coming into contact with the external environment, leading to sample contamination and reduced detection accuracy. Summary of the Invention

[0004] To achieve the above objectives, the present invention provides the following technical solution: A nucleic acid detection device includes: an integrated mechanism, a blocking mechanism, and a pushing mechanism. The integrated mechanism includes an integrated tube assembly, the inner wall of which is fitted with a sleeve. The blocking mechanism is located inside the integrated mechanism and includes a swab tube silicone plug. A lysis tube silicone plug is provided on one side of the swab tube silicone plug. The swab tube silicone plug and the lysis tube silicone plug are disposed inside the sleeve. Two blocking mechanisms are provided inside the swab tube silicone plug. A liquid storage groove is formed on the inner wall of the swab tube silicone plug. The pushing mechanism is located inside the blocking mechanism and includes a push rod. A liquid storage groove is formed at the end of the push rod.

[0005] The aforementioned technical solution describes a nucleic acid testing device that utilizes a combined, barrier, and propulsion mechanism. With a sheath as the core supporting structure, it precisely limits the positioning of the swab tube and the silicone stopper of the lysis tube. The dual-barrier design enables step-by-step control of the liquid path, and the cooperation between storage tank one and storage tank two ensures accurate temporary storage and transfer of liquid, reducing sample loss and improving testing efficiency and reliability.

[0006] As a further description of the above technical solution: The integrated tube assembly includes tube one, tube two, and tube three, which are collectively disposed on the outside of the sleeve.

[0007] The above technical solution uses a three-tube assembly with a sleeve to form an integrated layout, corresponding to the core processes of sampling, pyrolysis, and detection. This integrated design replaces the dispersed tubes, simplifying operation, shortening the liquid transfer path, reducing the risk of tube collision damage, and improving the device's practicality.

[0008] As a further description of the above technical solution: A connecting plate 1 is provided between pipe 1 and pipe 2, and a connecting plate 2 is provided between pipe 2 and pipe 3.

[0009] In the above technical solution, connecting plate one and connecting plate two are respectively connected to adjacent pipes to form a stable integrated structure. This can offset the impact of external forces during operation, avoid liquid path misalignment caused by pipe displacement, ensure smooth liquid transfer between pipes, and provide structural support for the stability of the testing process.

[0010] As a further description of the above technical solution: The top of the first pipe is provided with a rotating cover, the top of the second pipe is provided with a rotating cover, and the top of the third pipe is provided with a rotating cover.

[0011] The aforementioned technical solution features rotating caps at the top of each tube that can be opened and closed flexibly. When open, they facilitate precise sample addition and subsequent maintenance; when closed, they create a sealed environment. This prevents sample and reagent evaporation, isolates external contamination, and avoids liquid splashing during operation, thus improving testing safety.

[0012] As a further description of the above technical solution: The blocking mechanism also includes a limiting plate, which is disposed at the end of the sleeve.

[0013] In the above technical solution, the limiting plate is fixed to the end of the sleeve, and the position of the silicone plug is locked by mechanical limiting. This prevents the silicone plug from shifting when the push rod is pushed, ensuring precise alignment between the blocking structure and the tip of the push rod, thus guaranteeing the timeliness and accuracy of liquid circuit switching and improving the operating precision of the device.

[0014] As a further description of the above technical solution: The barrier mechanism also includes two secondary blocking devices, which are disposed inside the silicone plug of the pyrolysis tube.

[0015] The above technical solution uses a double-blocking, two-stage liquid barrier within the silicone plug of the lysis tube. This barrier, in conjunction with the push rod, enables stepwise liquid release, which strictly controls the lysis reaction time and prevents unresolved liquid from flowing into subsequent stages, effectively improving the sufficiency of nucleic acid lysis and the accuracy of detection results.

[0016] As a further description of the above technical solution: The pushing mechanism also includes a force-applying plate, which is disposed at the end of the push rod. The surface of the force-applying plate is provided with friction texture, and the end of the push rod away from the force-applying plate is spiked.

[0017] The above technical solution increases the friction of the hand gripping surface with the friction texture of the force plate, making the thrust easier to transmit and less prone to slippage. The spike-like end of the push rod can efficiently pierce the blocking structure, reducing the required operating force and making it easier for medical staff to operate. At the same time, it reduces the risk of device damage caused by uneven force application.

[0018] As a further description of the above technical solution: The sleeve has an adapter groove inside, which engages with the spikes on the push rod.

[0019] The above technical solution features a precise meshing between the adapter groove and the tip of the push rod, forming a guiding constraint structure. This structure restricts the movement trajectory of the push rod, preventing deviation that could lead to puncture failure or liquid leakage. It ensures that each step of the push rod's movement is precise and complete, providing a guarantee for the orderly connection of each process.

[0020] The present invention has the following beneficial effects: 1. In this invention, when the push rod of the pushing mechanism is smoothly advanced into the integrated tube assembly of the integrated mechanism, the silicone plugs of the swab tube and the lysis tube in the blocking mechanism will remain in a stable position under the precise limiting of the sleeve. The push rod can smoothly pierce the first blockage inside the silicone plug of the swab tube. At this time, the first liquid storage tank can reliably temporarily store the liquid, while the second liquid storage tank on the push rod can accurately receive and temporarily store the liquid, preparing for subsequent processes. This structure achieves the effect of reducing experimental steps while maintaining the same experimental results. It solves the problem in the prior art that nucleic acid detection requires frequent operation of the lysis tube, dilution tube, and reaction tube, and frequent opening of the tube cap and addition operations. This results in numerous experimental steps, high operational difficulty, extreme inconvenience, sample contamination, and reduced detection accuracy.

[0021] 2. In this invention, the device, composed of an integrated mechanism, a blocking mechanism, and a pushing mechanism, is small in size and lightweight, achieving the effect of occupying little space and being easy to carry. This solves the problem that nucleic acid detection devices in the prior art occupy a lot of space and are inconvenient to carry. Attached Figure Description

[0022] Figure 1 This is a perspective view of the present invention; Figure 2 This is an exploded view of the present invention; Figure 3 This is a cross-sectional view of the present invention; Figure 4 This is a top view of the present invention.

[0023] Figure label: 10. Connecting mechanism; 11. Pipe 1; 12. Pipe 2; 13. Pipe 3; 14. Connecting plate 1; 15. Connecting plate 2; 16. Rotating cover 1; 17. Rotating cover 2; 18. Rotating cover 3; 20. Barrier mechanism; 21. Silicone plug for swab tube; 22. Silicone plug for lysis tube; 23. Sleeve; 24. Limiting plate; 25. Blocking mechanism one; 26. Blocking mechanism two; 27. Liquid storage tank one; 28. Adaptor tank; 30. Pushing mechanism; 31. Push rod; 32. Force plate; 33. Liquid storage tank 2. Detailed Implementation

[0024] The following specific examples illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention.

[0025] Reference Figures 1 to 4An embodiment of the present invention provides a nucleic acid detection device, comprising: a connected mechanism 10, a blocking mechanism 20, and a pushing mechanism 30. The connected mechanism 10 includes a connected tube assembly, the inner wall of which is provided with a sleeve 23. The blocking mechanism 20 is located inside the connected mechanism 10 and includes a swab tube silicone plug 21. A lysis tube silicone plug 22 is provided on one side of the swab tube silicone plug 21. The swab tube silicone plug 21 and the lysis tube silicone plug 22 are located inside the sleeve 23. Two blocking devices 25 are provided inside the swab tube silicone plug 21. A liquid storage groove 27 is provided on the inner wall of the swab tube silicone plug 21. The pushing mechanism 30 is located inside the blocking mechanism 20 and includes a push rod 31. A liquid storage groove 33 is provided at the end of the push rod 31.

[0026] In the above embodiment, when the push rod 31 of the pushing mechanism 30 is smoothly pushed into the integrated tube assembly of the integrated mechanism 10, the silicone plug 21 of the swab tube and the silicone plug 22 of the pyrolysis tube in the blocking mechanism 20 will maintain a stable position under the precise limit of the sleeve 23. The push rod 31 can smoothly pierce the first blockage 25 in the silicone plug 21 of the swab tube. At this time, the first liquid storage tank 27 can reliably store the liquid, while the second liquid storage tank 33 of the push rod 31 can accurately receive and temporarily store the liquid, preparing for the subsequent process.

[0027] Reference Figures 1 to 4 The integrated pipe assembly includes pipe 11, pipe 22, and pipe 313, which are all disposed on the outside of the sleeve 23.

[0028] In the above embodiment, tube 11, tube 22, and tube 313 of the integrated tube assembly are integrated and compactly combined with the external sleeve 23. This not only optimizes the overall structural layout of the device, but also constructs an orderly liquid transfer pathway, enabling the liquid to flow precisely and gradually between tube 11, tube 22, and tube 313 according to the sampling, lysis, and amplification process of nucleic acid detection.

[0029] Reference Figure 3 A connecting plate 14 is provided between pipe 11 and pipe 22, and a connecting plate 25 is provided between pipe 22 and pipe 33.

[0030] In the above embodiments, the connecting plate 14 between tube 11 and tube 212, and the connecting plate 25 between tube 212 and tube 313, can firmly maintain the relative positions of each tube and prevent the tubes from shifting or misaligning during operation. This ensures the stable and smooth flow of liquid between the tubes and lays the foundation for the smooth progress of the subsequent nucleic acid testing process.

[0031] Reference Figure 1 , Figure 2The top of pipe 11 is provided with a rotating cover 16, the top of pipe 212 is provided with a rotating cover 217, and the top of pipe 313 is provided with a rotating cover 318.

[0032] In the above embodiments, the rotating cap 16 of tube 11, the rotating cap 17 of tube 2, and the rotating cap 18 of tube 3 can all be opened and closed flexibly. This facilitates the precise addition of samples, reagents, and other materials to the corresponding tubes in the early stages, and also allows for the opening and closing of the tubes as needed in later operations. At the same time, it can effectively reduce the evaporation of liquid inside the tubes and external contamination, ensuring the accuracy of nucleic acid testing.

[0033] Reference Figure 2 , Figure 4 The blocking mechanism 20 also includes a limiting plate 24, which is disposed at the end of the sleeve 23.

[0034] In the above embodiment, the limiting plate 24 of the blocking mechanism 20 forms a stable limiting support by precisely positioning the end of the sleeve 23. This positioning function can strictly limit the movement range of the silicone plug 21 of the swab tube and the silicone plug 22 of the pyrolysis tube, preventing them from shifting during liquid transfer or operation of the push rod 31, thus providing a reliable guarantee for the accuracy of liquid circuit switching.

[0035] Reference Figure 2 , Figure 3 The barrier mechanism 20 also includes two second-stage blocking devices 26, which are disposed inside the silicone plug 22 of the pyrolysis tube.

[0036] In the above embodiment, the limiting plate 24 of the blocking mechanism 20 forms a stable limiting support by precisely positioning the end of the sleeve 23, strictly limiting the movement range of the silicone plug 21 of the swab tube and the silicone plug 22 of the lysis tube. When the push rod 31 moves, the stable silicone plug can accurately pierce the second blocking 26 inside the silicone plug 22 of the lysis tube, smoothly completing the switching of the liquid transfer node to the subsequent tube.

[0037] Reference Figure 1 , Figure 2 The pushing mechanism 30 also includes a force plate 32, which is disposed at the end of the push rod 31. The surface of the force plate 32 is provided with friction texture, and the end of the push rod 31 away from the force plate 32 is spike-shaped.

[0038] In the above embodiment, the limiting plate 24 of the barrier mechanism 20 forms a stable limiting support by precisely positioning the end of the sleeve 23, strictly limiting the movement range of the silicone plug 21 of the swab tube and the silicone plug 22 of the pyrolysis tube. During operation, pushing the force plate 32, the friction texture on its surface can increase the stability of the thrust, thereby driving the push rod 31 to move smoothly; with the stable support of the silicone plug, the spiked end of the push rod 31 can accurately pierce the barrier structure, and the liquid storage tank 33 at its end can simultaneously realize the temporary storage of liquid, preparing for subsequent transfer.

[0039] Reference Figure 2 , Figure 3 The sleeve 23 has an adapter groove 28 inside, which engages with the spikes on the push rod 31.

[0040] In the above embodiment, the limiting plate 24 of the barrier mechanism 20 forms a stable limiting support by precisely positioning the end of the sleeve 23, strictly limiting the movement range of the silicone plug 21 of the swab tube and the silicone plug 22 of the pyrolysis tube. During operation, the force plate 32 is pushed, and the friction texture on its surface can increase the stability of the thrust, thereby driving the push rod 31 to move smoothly. The spiked end of the push rod 31 engages with the matching groove 28 of the sleeve 23 to ensure the positional accuracy during movement and avoid deviation. With the stable support of the silicone plug and the guiding effect of the matching groove 28, the spiked end of the push rod 31 can accurately pierce the barrier structure, and the liquid storage tank 33 at its end can simultaneously realize the temporary storage of liquid, preparing for subsequent transfer.

[0041] Working principle: When using this nucleic acid detection device, the initial preparation is based on the integrated tube assembly consisting of tube 11, tube 22, and tube 33. Connecting plate 14 and connecting plate 25 ensure the relative position stability of the three functional tubes. The rotating caps 16, 27, and 38 on the top can flexibly assist in accurate sample addition. The sleeve 23 outside the integrated tube assembly not only integrates the tubes, but its end limiting plate 24 can also accurately position the silicone plugs 21 of the internal swab tube and silicone plugs 22 of the lysis tube. In the operation phase, pushing the force plate 32 with friction texture will drive the push rod 31 to move smoothly along the adapter groove 28 of the sleeve 23. The pointed tip first punctures the first blockage 25 of the silicone stopper 21 of the swab tube. At this time, the liquid in the swab tube flows into the second storage tank 33 of the push rod 31 through the first storage tank 27. After the push rod 31 continues to puncture the other blockage 25 of the silicone stopper 21 of the swab tube, the liquid enters the second tube 12 to complete the lysis step. Then the push rod 31 further punctures the second blockage 26 of the silicone stopper 22 of the lysis tube and sends the liquid into the third tube 13. Finally, the push rod 31 is rotated 180 degrees, and the residual liquid in the second storage tank 33 will be poured onto the lyophilized reagent in the third tube 13, thereby advancing the subsequent detection process. The device adopts a modular integrated design and can realize the integration and convenience of sample processing without the need for complex instruments.

[0042] The above embodiments are merely illustrative of the technical solutions of the present invention and are not intended to limit it. Anyone skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A nucleic acid detection device, characterized in that, include: Connecting mechanism (10), blocking mechanism (20), and pushing mechanism (30); The integrated mechanism (10) includes an integrated tube assembly, and a sleeve (23) is provided through the inner wall of the integrated tube assembly. A barrier mechanism (20) is provided inside the integrated mechanism (10). The barrier mechanism (20) includes a swab tube silicone plug (21). A pyrolysis tube silicone plug (22) is provided on one side of the swab tube silicone plug (21). The swab tube silicone plug (21) and the pyrolysis tube silicone plug (22) are provided inside the sleeve (23). Two blocking devices (25) are provided inside the swab tube silicone plug (21). A liquid storage tank (27) is opened on the inner wall of the swab tube silicone plug (21). A pushing mechanism (30) is provided inside the barrier mechanism (20). The pushing mechanism (30) includes a push rod (31), and the end of the push rod (31) is provided with a liquid storage tank (33).

2. The nucleic acid detection device according to claim 1, characterized in that: The integrated tube assembly includes tube one (11), tube two (12), and tube three (13), which are arranged together on the outside of the sleeve (23).

3. The nucleic acid detection device according to claim 2, characterized in that: A connecting plate 1 (14) is provided between the first pipe (11) and the second pipe (12), and a connecting plate 2 (15) is provided between the second pipe (12) and the third pipe (13).

4. The nucleic acid detection device according to claim 3, characterized in that: The top of the first tube (11) is provided with a rotating cover first (16), the top of the second tube (12) is provided with a rotating cover second (17), and the top of the third tube (13) is provided with a rotating cover third (18).

5. The nucleic acid detection device according to claim 1, characterized in that: The blocking mechanism (20) also includes a limiting plate (24), which is disposed at the end of the sleeve (23).

6. A nucleic acid detection device according to claim 1, characterized in that: The barrier mechanism (20) further includes two second-stage blocking devices (26), which are disposed inside the silicone plug (22) of the pyrolysis tube.

7. A nucleic acid detection device according to claim 1, characterized in that: The pushing mechanism (30) also includes a force plate (32), which is disposed at the end of the push rod (31). The surface of the force plate (32) is provided with friction texture, and the end of the push rod (31) away from the force plate (32) is spike-shaped.

8. A nucleic acid detection device according to claim 1, characterized in that: The sleeve (23) has an adapter groove (28) inside, which engages with the spikes on the push rod (31).

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