Food microorganism detection device and detection method thereof

Abstract

The invention relates to the field of food microorganism detection, and particularly discloses a food microorganism detection device and a detection method thereof.The food microorganism detection device comprises an outer detection mechanism and a rotary detection table mechanism; the outer detection mechanism comprises an outer fixing frame assembly, and a bacterial colony counting assembly is fixedly mounted on the outer side of the outer fixing frame assembly; the rotary detection table mechanism comprises an outer support frame assembly, and a rotary detection table assembly is fixedly mounted in the middle of the outer support frame assembly; the outer supporting frame assembly is clamped and embedded in the middle of the outer fixing frame assembly. Through automatic rotation of the rotary detection table assembly, rapid placement of test pieces, sample dropwise adding and detection position transferring are achieved, the detection efficiency is remarkably improved, after culture is completed, the rotary detection table assembly can automatically convey the test pieces carrying the samples to the position below the bacterial colony counting assembly, and the detection efficiency is further improved.

Description

Technical Field

[0001] This invention belongs to the field of food microbiology detection, specifically a food microbiology detection device and its detection method. Background Technology

[0002] Food microbiological testing is a core part of food safety control. It assesses food hygiene quality and spoilage risk by quantitatively analyzing microbial indicators such as total bacterial count and coliform bacteria in food.

[0003] The test strip uses non-woven fabric, paper film or film as a carrier, and pre-filled with dry culture medium (containing nutrients, coagulant and color developer). Microbial metabolites react with the color developer to form observable colonies.

[0004] Traditional techniques rely on manually placing test pieces, adding samples, and transferring detection sites, which is cumbersome and time-consuming. Summary of the Invention

[0005] To address the aforementioned technical problems, this invention provides a food microbial detection device to solve the problem that traditional technologies rely on manually placing test strips, adding samples, and transferring detection sites, which is cumbersome and time-consuming.

[0006] A food microbial detection device includes an external detection mechanism and a rotating detection stage mechanism;

[0007] The external detection mechanism includes an external fixation frame assembly, on the outside of which a colony counting component is fixedly installed;

[0008] The rotating testing table mechanism includes an outer support frame assembly, and the rotating testing table assembly is fixedly installed in the middle of the outer support frame assembly;

[0009] The external support frame assembly is snapped into the middle position of the external fixing frame assembly;

[0010] The external fixation frame assembly includes an external fixation frame body, and a sample placement stage is fixedly installed on the upper part of the outer side of the external fixation frame body;

[0011] The colony counting component includes an external fixing frame, a screw, a connecting frame, and a camera. A guide rod is fixedly installed on the outside of the external fixing frame via the connecting frame. A motor is fixedly installed at one end of the screw via a coupling. A light shield is fixedly installed at one end of the connecting frame, and a supplementary light is fixedly installed at the lower end of the light shield.

[0012] The external fixing frame is fixedly installed on the outside of the external fixing frame body by bolts. The lead screw is fixedly installed on the external fixing frame by a shaft. The motor is fixedly installed on the external fixing frame. The connecting frame is limited and slidably embedded in the guide rod. The lead screw drives the connecting frame to move up and down along the guide rod by a matching slider. The camera is fixedly installed on the light shield.

[0013] Preferably, a recycling platform is also fixedly installed on the outer side of the external fixing frame body, and a guide slope is provided on the recycling platform.

[0014] Preferably, a mounting bracket is fixedly installed on the upper edge of the outer side of the recycling platform;

[0015] A guide assembly is also fixedly installed on the outside of the recycling station;

[0016] The guiding assembly includes an L-shaped guide frame and a second motor. A shaft is fixedly installed at one end of the L-shaped guide frame, and a pulley is fixedly installed at the end of the shaft. A pulley is fixedly installed at the output end of the second motor, and a belt is fitted on the outer sides of both pulleys.

[0017] The second motor is fixedly installed on the outside of the recycling platform, the shaft is fixedly installed on the mounting frame by bearings, and the L-shaped guide frame is L-shaped.

[0018] Preferably, the outer support frame assembly includes an outer bracket, an edge platform is fixedly installed on the upper side of the outer bracket, and an inner bracket is fixedly installed at the upper end of the inner side of the outer bracket;

[0019] The rotating testing stage assembly is fixedly installed on the inner support.

[0020] Preferably, the rotating testing table assembly includes a third motor and a rotating disk, a reducer is fixedly installed at the output end of the third motor, and a plurality of sterile tables are placed on the rotating disk;

[0021] The motor is fixedly installed at the bottom of the edge platform, the reducer is fixedly installed on the upper side of the inner bracket, and the rotating disk is fixedly installed on the upper side of the reducer. The rotating disk and the edge platform are on the same plane.

[0022] Preferably, a numbered block is also fixedly installed on one side of the rotating disk located on the sterile table;

[0023] The number and location of the numbered blocks and sterile stations correspond to each other.

[0024] A method for detecting microorganisms in food, comprising the aforementioned food microorganism detection device, and further comprising the following steps:

[0025] S1. Temporarily place the test strips on the sample placement stage, control the intermittent rotation of the rotating detection stage assembly, and place the test strips one by one on the sterile stage of the rotating detection stage assembly.

[0026] S2. Control the rotating detection stage assembly to rotate intermittently, and then drip the sample one by one onto the test piece after drawing it up with a pipette;

[0027] S3. Incubate the test piece with the sample added for 24 hours.

[0028] S4. After the culture is completed, control the rotating detection stage assembly to rotate intermittently so that the test piece with the sample added rotates to the underside of the light shield.

[0029] S5. Control the motor to drive the lead screw to rotate, and drive the connecting frame to descend vertically along the guide rod through the threaded transmission, so that the light shield moves down and covers the test piece on which the sample is dropped, and at the same time turn on the supplementary light for supplementary lighting;

[0030] S6. Take a picture of the colony count on the test strip using a camera and transmit the picture to the colony counter for counting;

[0031] S7. After the test is completed, control motor 2 starts, and drives the L-shaped guide frame to rotate through pulley 1, pulley 2 and belt drive shaft.

[0032] S8. When the L-shaped guide frame rotates onto the rotating detection stage assembly, control the rotating detection stage assembly to continue rotating, so that the sterile table is transferred to the recycling table along the guide slope under the guidance of the L-shaped guide frame, thereby realizing the rapid recycling of the sterile table.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] The automated rotation of the rotating detection stage assembly enables rapid placement of test strips, sample addition, and transfer of detection positions, significantly improving detection efficiency. After incubation, the rotating detection stage assembly can automatically transport the test strips carrying the samples to the bottom of the colony counting assembly, further improving detection efficiency.

[0035] The colony counting component adopts a screw-driven vertical descent design for the connecting frame, ensuring precise alignment between the light shield and the test strip. Combined with the stable light source provided by the supplementary light, the camera can capture clear images of the test strip, thereby improving the accuracy of colony counting.

[0036] The design of the sample placement and recovery station makes the temporary placement and recovery of test strips extremely convenient. In particular, the guide ramp and guide components on the recovery station enable rapid recovery of the sterile station through the automatic rotation of the L-shaped guide frame. Attached Figure Description

[0037] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0038] Figure 2 This is an exploded structural diagram of the present invention;

[0039] Figure 3 This is a schematic diagram of the external detection mechanism of the present invention;

[0040] Figure 4 This is a schematic diagram of the colony counting component of the present invention;

[0041] Figure 5 This is a schematic diagram of the structure of the guiding component of the present invention;

[0042] Figure 6 This is a schematic diagram of the rotating detection stage mechanism of the present invention;

[0043] Figure 7 This is a schematic diagram of the structure of the rotating detection stage assembly of the present invention.

[0044] In the diagram: 1. External detection mechanism; 11. External fixing frame assembly; 111. External fixing frame body; 112. Sample placement stage; 113. Recovery stage; 114. Guide slope; 115. Mounting frame; 12. Colony counting assembly; 121. External fixing frame; 122. Lead screw; 123. Connecting frame; 124. Camera; 125. Guide rod; 126. Motor 1; 127. Light shield; 128. Supplemental light; 13. Guide assembly; 131. 1. L-shaped guide frame; 132. Motor II; 133. Shaft; 134. Pulley I; 135. Pulley II; 136. Belt; 2. Rotary testing table mechanism; 21. Outer support frame assembly; 211. Outer support; 212. Edge table surface; 213. Inner support; 22. Rotary testing table assembly; 221. Motor III; 222. Rotary disk; 223. Reducer; 224. Sterile table; 225. Numbered block; 226. Test piece. Detailed Implementation

[0045] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0046] like Figures 1 to 4 As shown:

[0047] Example 1: The present invention provides a food microbial detection device, including an external detection mechanism 1 and a rotating detection stage mechanism 2;

[0048] The external testing mechanism 1 includes an external fixation frame assembly 11, on the outside of which a colony counting component 12 is fixedly installed;

[0049] The rotating testing table mechanism 2 includes an outer support frame assembly 21, and a rotating testing table assembly 22 is fixedly installed in the middle of the outer support frame assembly 21.

[0050] The outer support frame assembly 21 is snapped into the middle position of the outer fixing frame assembly 11;

[0051] The external fixation frame assembly 11 includes an external fixation frame body 111, and a sample placement platform 112 is fixedly installed on the upper side of the external fixation frame body 111.

[0052] The colony counting component 12 includes an outer fixing frame 121, a screw rod 122, a connecting frame 123, and a camera 124. A guide rod 125 is fixedly installed on the outer side of the outer fixing frame 121 through the connecting frame. A motor 126 is fixedly installed at one end of the screw rod 122 through a coupling. A light shield 127 is fixedly installed at one end of the connecting frame 123. A supplementary light 128 is fixedly installed at the lower end of the light shield 127.

[0053] The outer fixing frame 121 is fixedly installed on the outside of the outer fixing frame body 111 by bolts. The lead screw 122 is fixedly installed on the outer fixing frame 121 by a shaft. The motor 126 is fixedly installed on the outer fixing frame 121. The connecting frame 123 is limited and slidably embedded in the guide rod 125. The lead screw 122 drives the connecting frame 123 to move up and down along the guide rod 125 through a matching slider. The camera 124 is fixedly installed on the light shield 127.

[0054] Among them, the sample placement stage 112 can facilitate the temporary placement of test pieces and samples;

[0055] Control the rotation of the rotating testing stage assembly 22 and place the test strips one by one onto the sterile stage on the rotating testing stage assembly 22;

[0056] The rotating detection stage assembly 22 is controlled to rotate and samples are drawn up by a pipette and then dripped onto the test piece one by one.

[0057] A temperature-adjustable transparent cover can be placed on top, and after culturing at 36℃±1℃ for 24 hours, the rotating detection stage assembly 22 is controlled to rotate so that the test piece with the sample added rotates to the underside of the light-blocking cover 127.

[0058] The control motor 126 drives the lead screw 122 to rotate, which drives the connecting frame 123 to descend vertically along the guide rod 125 through the thread transmission, so that the light shield 127 moves down and covers the test strip on which the sample is dropped. The supplementary light 128 can be turned on to provide supplementary light, and the camera 124 can take pictures of the number of colonies on the test strip. The camera 124 is connected to the colony counter for counting.

[0059] The outer fixing frame body 111 serves as the overall support structure. The outer fixing frame 121, which is fixed by bolts, ensures the installation stability of the colony counting component 12. The limiting sliding structure ensures the movement accuracy of the connecting frame 123.

[0060] like Figure 3 and Figure 5 As shown:

[0061] Example 2: This example is basically the same as the previous example, except that it also includes a recycling platform 113 fixedly installed on the outside of the outer fixing frame body 111, and a guide slope 114 is provided on the recycling platform 113.

[0062] Specifically, a mounting bracket 115 is fixedly installed on the upper edge of the outer side of the recycling table 113;

[0063] A guide assembly 13 is also fixedly installed on the outside of the recycling table 113;

[0064] The guide assembly 13 includes an L-shaped guide frame 131 and a second motor 132. A shaft 133 is fixedly installed at one end of the L-shaped guide frame 131, and a pulley 134 is fixedly installed at the end of the shaft 133. A pulley 135 is fixedly installed at the output end of the second motor 132. A belt 136 is fitted on the outer side of both pulley 134 and pulley 135.

[0065] Motor 132 is fixedly installed on the outside of the recycling table 113, and shaft 133 is fixedly installed on the mounting frame 115 through bearings. The L-shaped guide frame 131 is L-shaped.

[0066] As can be seen from the above, during operation, the test strip is first temporarily placed on the sample placement stage 112, and the rotating detection stage assembly 22 is rotated in a controlled manner to place the test strips one by one on the sterile stage of the detection position; then the pipette is used to accurately pick up the sample and add it drop by drop to the surface of the test strip.

[0067] After 24 hours of incubation, the rotating detection stage assembly 22 rotates again, delivering the test strips containing the samples one by one to the area below the colony counting assembly 12.

[0068] At this time, motor 126 drives lead screw 122 to rotate, and through thread transmission, connecting frame 123 is driven to descend vertically along guide rod 125, so that light shield 127 covers the test piece. At the same time, supplement light 128 is turned on to provide a stable light source, and camera 124 takes high-definition pictures of the test piece and transmits them to colony counter to complete the counting.

[0069] After the test is completed, the control motor 132 drives the L-shaped guide frame 131 to rotate through the pulley 134, the second pulley 135, the belt 136 and the shaft 133;

[0070] When the L-shaped guide frame 131 rotates onto the rotary testing table assembly 22, the rotary testing table assembly 22 continues to work, and the sterile table will be transferred to the recovery table 113 along the guide slope 114 under the guidance of the L-shaped guide frame 131.

[0071] This allows for the rapid retrieval of the sterile worktable, making it convenient for staff to clean it.

[0072] like Figure 6 and Figure 7 As shown:

[0073] Example 3: This example is basically the same as the previous example, except that it also includes an outer support frame assembly 21, which includes an outer support 211. An edge platform 212 is fixedly installed on the upper side of the outer support 211, and an inner support 213 is fixedly installed on the upper part of the outer support 211.

[0074] The rotating testing stage assembly 22 is fixedly installed on the inner bracket 213.

[0075] Specifically, the rotating testing table assembly 22 includes a motor 221 and a rotating disk 222. A reducer 223 is fixedly installed at the output end of the motor 221, and several sterile tables 224 are placed on the rotating disk 222.

[0076] Motor 221 is fixedly installed at the bottom of edge platform 212, reducer 223 is fixedly installed on the upper side of inner bracket 213, and rotating disk 222 is fixedly installed on the upper side of reducer 223. Rotating disk 222 and edge platform 212 are on the same plane.

[0077] Specifically, a numbered block 225 is also fixedly installed on one side of the aseptic table 224 on the rotating tray 222;

[0078] The number and position of block 225 and sterile table 224 correspond.

[0079] As can be seen from the above, the outer support 211 provides support for the rotating detection table assembly 22 through the inner support 213 at the upper end of the inner support;

[0080] After the motor 221 is started, its output end drives the rotating disk 222 to rotate at a constant speed through the reducer 223, so that the sterile table 224 and the corresponding numbered block 225 fixed on the rotating disk 222 rotate synchronously.

[0081] Block 225 allows staff to easily identify the sample drop location.

[0082] All standard parts used in this invention can be purchased commercially, and irregularly shaped parts can be customized according to the description and drawings. The specific connection methods for each part all employ conventional methods such as bolts, rivets, and welding, which are mature technologies in the prior art. The machinery, parts, and equipment all use conventional models in the prior art, and the circuit connections also use conventional connection methods in the prior art, which will not be detailed here. Any content not described in detail in this specification belongs to the prior art known to those skilled in the art.

[0083] In the description of this invention, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. "A plurality of" means two or more, unless otherwise explicitly specified.

[0084] In this invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0085] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "over," and "on top" of the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0086] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0087] The accompanying drawings of the embodiments disclosed in this invention only involve structures relevant to the embodiments disclosed in this invention. Other structures can be referred to with common designs. Unless otherwise specified, the same embodiment and different embodiments of this invention can be combined with each other.

[0088] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A food microbial detection device, characterized in that, It includes an external inspection mechanism (1) and a rotating inspection table mechanism (2); The external detection mechanism (1) includes an external fixation frame assembly (11), on the outside of which a colony counting component (12) is fixedly installed. The rotating testing table mechanism (2) includes an outer support frame assembly (21), and a rotating testing table assembly (22) is fixedly installed in the middle of the outer support frame assembly (21). The outer support frame assembly (21) is snapped into the middle position of the outer fixing frame assembly (11); The external fixation frame assembly (11) includes an external fixation frame body (111), and a sample placement stage (112) is fixedly installed on the upper part of the outer side of the external fixation frame body (111). The colony counting component (12) includes an outer fixing frame (121), a screw (122), a connecting frame (123), and a camera (124). A guide rod (125) is fixedly installed on the outer side of the outer fixing frame (121) through the connecting frame. A motor (126) is fixedly installed at one end of the screw (122) through a coupling. A light shield (127) is fixedly installed at one end of the connecting frame (123). A supplementary light (128) is fixedly installed at the lower end of the light shield (127). The outer fixing frame (121) is fixedly installed on the outside of the outer fixing frame body (111) by bolts. The lead screw (122) is fixedly installed on the outer fixing frame (121) by a shaft. The motor (126) is fixedly installed on the outer fixing frame (121). The connecting frame (123) is limited and slidably embedded in the guide rod (125). The lead screw (122) drives the connecting frame (123) to move up and down along the guide rod (125) by a matching slider. The camera (124) is fixedly installed on the light shield (127).

2. The food microbial detection device as described in claim 1, characterized in that, A recovery platform (113) is also fixedly installed on the outside of the outer fixed frame body (111), and a guide slope (114) is provided on the recovery platform (113).

3. The food microbial detection device as described in claim 2, characterized in that, An installation frame (115) is fixedly installed on the upper edge of the outer side of the recycling platform (113). A guide assembly (13) is also fixedly installed on the outside of the recycling station (113). The guide assembly (13) includes an L-shaped guide frame (131) and a second motor (132). A shaft (133) is fixedly installed at one end of the L-shaped guide frame (131), and a pulley (134) is fixedly installed at the end of the shaft (133). A pulley (135) is fixedly installed at the output end of the second motor (132). A belt (136) is fitted on the outer side of both the pulley (134) and the pulley (135). The second motor (132) is fixedly installed on the outside of the recycling platform (113), the shaft (133) is fixedly installed on the mounting frame (115) by bearings, and the L-shaped guide frame (131) is L-shaped.

4. The food microbial detection device as described in claim 1, characterized in that, The outer support frame assembly (21) includes an outer support (211), an edge platform (212) is fixedly installed on the upper side of the outer support (211), and an inner support (213) is fixedly installed on the upper inside of the outer support (211). The rotating testing stage assembly (22) is fixedly installed on the inner bracket (213).

5. The food microbial detection device as described in claim 4, characterized in that, The rotating testing stage assembly (22) includes a motor (221) and a rotating disk (222). A reducer (223) is fixedly installed at the output end of the motor (221), and several sterile stations (224) are placed on the rotating disk (222). The motor (221) is fixedly installed at the bottom of the edge platform (212), the reducer (223) is fixedly installed on the upper side of the inner bracket (213), the rotating disk (222) is fixedly installed on the upper side of the reducer (223), and the rotating disk (222) and the edge platform (212) are on the same plane.

6. The food microbial detection device as described in claim 5, characterized in that, A numbered block (225) is also fixedly installed on one side of the aseptic table (224) on the rotating disk (222); The number and position of the numbered blocks (225) and the sterile table (224) correspond to each other.

7. A method for detecting microorganisms in food, characterized in that, The food microbial detection device as described in any one of claims 5 or 6 further includes the following steps: S1. Temporarily place the test strips on the sample placement stage (112), control the rotating detection stage assembly (22) to rotate intermittently, and place the test strips one by one on the sterile stage (224) on the rotating detection stage assembly (22); S2. Control the rotating detection stage assembly (22) to rotate intermittently, and then drip the sample onto the test piece one by one after taking it in through the pipette; S3. Incubate the test piece with the sample added for 24 hours. S4. After the culture is completed, control the rotating detection stage assembly (22) to rotate intermittently so that the test piece with the sample added rotates to the underside of the light shield (127); S5. Control motor 1 (126) drives screw (122) to rotate, and through thread transmission, drives connecting frame (123) to descend vertically along guide rod (125), so that light shield (127) moves down and covers the test piece on which the sample is dropped, and at the same time turn on supplementary light (128) for supplementary light; S6. Take a picture of the colony count on the test strip using a camera (124) and transmit the picture to a colony counter for counting; S7. After the test is completed, control motor 2 (132) starts, drives shaft (133) to rotate through pulley 1 (134), pulley 2 (135) and belt (136), thereby driving L-shaped guide frame (131) to rotate; S8. When the L-shaped guide frame (131) rotates onto the rotating detection stage assembly (22), the rotating detection stage assembly (22) is controlled to continue rotating, so that the sterile table (224) is transferred to the recycling table (113) along the guide slope (114) under the guidance of the L-shaped guide frame (131), thereby realizing the rapid recycling of the sterile table (224).

Images