Process for preparation of 2, 5-furandicarboxylic acid
By combining NAD(P)+-dependent oxidoreductase and H2O-forming NAD(P)H oxidase, the problems of harsh reaction conditions and uneconomical use of cofactors in the preparation of FDCA in the existing technology are solved, and efficient and sustainable FDCA preparation is achieved.
Patent Information
- Application Number
- CN202480047630.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2023-12-22
- Filing Date
- 2024-12-23
- Publication Date
- 2026-03-20
AI Technical Summary
Existing chemical methods for preparing 2,5-furandicarboxylic acid (FDCA) suffer from problems such as harsh reaction conditions, numerous byproducts, and the use of expensive and toxic catalysts, while biocatalytic processes are uneconomical due to low substrate concentrations or large amounts of cofactors.
5-Formyl-2-furanoic acid (FFA) was oxidized in aqueous solution using an NAD(P)+-dependent oxidoreductase, and then oxidized back to NAD(P)+ with the assistance of H2O-forming NAD(P)H oxidase, reducing the amount of cofactor required. Aldehyde dehydrogenase was selected as the oxidoreductase, and high conversion rate was achieved by combining specific amino acid sequences and nucleic acid coding.
Achieving high conversion rates in FDCA preparation at high substrate concentrations reduces the use of cofactors and meets the requirements of sustainable development.