Method for increasing yield of calculus bovis through specific forage combination feeding

Abstract

The invention discloses a method for increasing the yield of bezoar through specific forage combination feeding. According to the method, through scientific treatment and directional feeding of the ox gall powder residues, appropriate physiological conditions are created for bezoar formation. Active ingredients in the treated residues are more easily absorbed by cattle, the gallbladder contraction rhythm is mildly adjusted, cholestasis is induced, and cholesterol is crystallized and separated out, which is the core mechanism of calculus bovis generation; meanwhile, due to the interval feeding rhythm, continuous stimulation is guaranteed, excessive burden cannot be caused to the hepatobiliary system of the cattle, the calculus bovis can be gradually formed in a stable physiological environment, the yield of the calculus bovis is increased from the source, and the double advantages of resource recycling and breeding health guarantee are achieved. Originally possibly discarded ox gall powder residues are converted into effective raw materials after being treated, the breeding cost is reduced, meanwhile, waste discharge is reduced, and the method is suitable for practical application of farmers.

Description

Technical Field

[0001] This invention belongs to the field of bezoar production technology, specifically a method for increasing bezoar production through feeding a specific combination of forage. Background Technology

[0002] Bezoar, a gallstone found in the gallbladder, bile duct, or hepatic duct of cattle (Bos taurus or buffalo), is an extremely precious and highly effective medicinal material in Traditional Chinese Medicine. Intact bezoars are typically oval, spherical, or irregularly spherical, with a golden-yellow or brownish-yellow surface that is fine-grained and slightly glossy, displaying a "patterned" appearance composed of various colors. Bezoar has a fragrant aroma, a bitter taste followed by a sweet aftertaste, and is cool in nature, primarily affecting the heart and liver meridians. Its core functions are clearing the heart and detoxifying, opening the orifices and resolving phlegm, calming wind and relieving convulsions. It is commonly used to treat critical conditions such as delirium due to fever, phlegm-induced coma due to stroke, convulsions, sore throat, oral ulcers, and carbuncles and boils. Due to its scarcity in natural production, it is extremely expensive. Artificially cultivated and synthesized bezoar are available as substitutes and are widely used in various precious traditional Chinese medicine preparations. Increasing bezoar production involves a series of artificial intervention technologies and methods developed to address the scarcity of natural bezoar resources, aiming to meet the huge demand in the pharmaceutical market. Artificial cultivation of bezoar involves surgically implanting a specially prepared foreign nucleus (or non-foreign nucleus) and bezoar fungi into the gallbladder of a cow to artificially induce gallstone formation. After a period of feeding, the cultivated bezoar is surgically removed. This method mimics the formation mechanism of natural bezoar, has a relatively short cycle, and significantly increases yield. In addition, using modern biotechnology to produce artificial bezoar through in vitro cell culture or chemical synthesis is also an important research approach. These technologies not only effectively alleviate the supply and demand imbalance of bezoar but also provide a scientific paradigm for the sustainable utilization of this rare medicinal material.

[0003] However, existing technologies for increasing bezoar production often suffer from low resource utilization and high burden on cattle: either they fail to effectively utilize potential raw materials such as bovine bile powder residue, leading to waste, or they use continuous high-intensity stimulation feeding methods, which increase the burden on the bovine liver and gallbladder system and cause health problems. Furthermore, they lack targeted treatment to improve the absorption of effective components, making it difficult to steadily increase bezoar production while ensuring the health of the herd. Summary of the Invention

[0004] The purpose of this invention is to provide a method for increasing bezoar production by feeding a specific combination of forages in order to solve the problems mentioned above.

[0005] The technical solution adopted in this invention is as follows: A method for increasing bezoar production through feeding a specific combination of forage, characterized in that the method includes the following steps: S1: Select healthy local yellow cattle aged 3-5 years (preferably Qinchuan cattle, Luxi yellow cattle, etc.), weighing 400-500kg, with no history of liver and gallbladder diseases; collect tail vein blood to test bilirubin and bile acid levels, record initial weight, feeding habits and fecal characteristics, and establish individual files.

[0006] S2: Feed the animal with regular basic feed (60% corn silage + 30% alfalfa + 10% wheat bran) for 15 consecutive days, and supplement with 50g of multivitamin and mineral premixed feed per animal per day; adjust the feed moisture to about 60% to ensure optimal palatability.

[0007] S3: Prepare the basic herbal feed for liver cleansing and bile promotion: Chop dandelion, crush artemisia capillaris, and mix them evenly with other herbs. Each kilogram of herbal feed contains 15g of dandelion and 5g of artemisia capillaris.

[0008] S4: Add 50g of high-temperature sterilized and lactic acid bacteria fermented bovine bile powder residue (to remove toxic components) to the S3 basic forage 3 times a week with an interval of 2 days. The free bile acids in the fermented residue can regulate the gallbladder contraction rhythm and gently induce bile stasis and cholesterol crystal precipitation.

[0009] S5: Feed three times a day: feed hay containing S4 additive at 7:00 am and 6:00 pm (stimulation day), and feed pure S3 basic hay at 12:00 pm; each feeding amount is 2.5%-3% of the cattle's body weight, ensuring that there is no leftover feed.

[0010] S6: Record feeding speed and fecal consistency daily (target is formed soft stool); test bilirubin and alanine aminotransferase levels weekly: reduce the amount of Artemisia capillaris when feces are dry and hard, and suspend S4 stimulation feeding for 1-2 weeks when bilirubin is abnormal.

[0011] S7: After 6 months of feeding, add vitamin E (200 IU / head / day) + astragalus polysaccharide (10g / head / day) to the basic feed to enhance the antioxidant capacity of liver cells and reduce gallbladder inflammation.

[0012] S8: After feeding for 12-15 months, stop feeding S4 stimulation feed and feed only S2 basic feed for 15 days; after confirming the size of the bezoar (≥20g) by ultrasound, perform minimally invasive surgery to collect it; feed S2 feed after surgery to promote the recovery of cattle.

[0013] In a preferred embodiment, in step S1, healthy local yellow cattle aged 3 to 5 years are selected, with preference given to Qinchuan cattle or Luxi yellow cattle. The cattle should weigh between 400 and 500 kg and have no history of liver or gallbladder disease. Blood samples are collected from the tail vein of each cow and sent to a testing institution to determine bilirubin and bile acid levels, ensuring both indicators are within the normal range. Simultaneously, the initial weight, daily feeding habits (including the number of times a day is fed and the duration of each feeding), and fecal characteristics (such as consistency and color) of each cow are recorded to establish an individual feeding record for each cow, allowing for targeted adjustments to the feeding program later.

[0014] In a preferred embodiment, in step S1, healthy local yellow cattle aged 3 to 5 years are selected, with preference given to Qinchuan cattle or Luxi yellow cattle. The cattle should weigh between 400 and 500 kg and have no history of liver or gallbladder disease. Blood samples are collected from the tail vein of each cow and sent to a testing institution to determine bilirubin and bile acid levels, ensuring both indicators are within the normal range. Simultaneously, the initial weight, daily feeding habits (including the number of times a day is fed and the duration of each feeding), and fecal characteristics (such as consistency and color) of each cow are recorded to establish an individual feeding file for each cow, allowing for targeted adjustments to the feeding program later.

[0015] In a preferred embodiment, in step S3, the basic forage includes: 50% corn silage, 25% alfalfa, 15% oat grass, 5% fresh dandelion, 3% dried artemisia capillaris, and 2% soybean meal.

[0016] In a preferred embodiment, in step S3, fresh dandelion is chopped into small pieces of about 1 cm, and dried artemisia capillaris is pulverized to 80 mesh. All raw materials are then placed in a forage mixer and mixed for 10 minutes to ensure uniform mixing. Each kilogram of prepared forage should contain 15 grams of fresh dandelion and 5 grams of dried artemisia capillaris. The amount of soybean meal added must be strictly controlled to supplement sufficient protein to support the daily metabolic needs of the cattle.

[0017] In a preferred embodiment, step S4 specifically includes the following steps: S4-1: Place the collected bovine bile powder residue into a sterilization device at 121℃ for 30 minutes to thoroughly remove potentially toxic components. S4-2: Add a special bacterial culture to the sterilized bovine bile powder residue, ferment at a constant temperature of 37℃ for 48 hours, and control the pH value at around 4.5 to improve the absorption efficiency of free bile acids. S4-3: Select 3 days per week for stimulating feeding (with a 2-day interval between two consecutive days), and add 50 grams of fermented bovine bile residue to each cow daily. S4-4: Mix the fermented bovine bile powder residue evenly into the liver-cleansing and bile-promoting basic feed for the day, and feed it at 7 am and 6 pm. The effective ingredients gently regulate the gallbladder contraction rhythm, inducing bile stasis and cholesterol crystal precipitation.

[0018] In a preferred embodiment, in step S5, forage is fed three times a day at 7:00 AM, 12:00 PM, and 6:00 PM. On stimulation days (3 days a week), a liver-cleansing and bile-promoting basic forage supplemented with fermented bovine bile powder residue is fed at 7:00 AM and 6:00 PM, while a pure liver-cleansing and bile-promoting basic forage is fed at 12:00 PM. On non-stimulation days, pure liver-cleansing and bile-promoting basic forage is fed all three times. The amount fed each time is calculated based on the cattle's weight, ranging from 2.5% to 3% of their body weight. For example, a 400 kg cattle is fed 10 to 12 kg of forage each time to ensure that the cattle can fully consume the feed and avoid waste.

[0019] In a preferred embodiment, in step S6, the feeding rate (time from the start of feeding to complete consumption) and fecal consistency (target: formed, soft stool) of each cow are observed and recorded daily. Tail vein blood samples are collected weekly to test bilirubin and alanine aminotransferase (ALT) levels. If the cow's feces are found to be hard and dry, the proportion of dried Artemisia capillaris in the basal feed should be reduced from 3% to 2%. If bilirubin levels are abnormally high, targeted stimulation feeding should be suspended for 1 to 2 weeks until the levels return to normal before resuming. If ALT levels are abnormal, the amount of soybean meal added should be reduced by 0.5%, and the proportion of corn silage increased by 0.5%.

[0020] In a preferred embodiment, in step S7, after 6 months of continuous feeding of liver-cleansing and bile-promoting basic forage and targeted stimulation feeding, additional nutritional fortifiers are added to the basic forage. Each cow is supplemented with 200 IU of vitamin E daily, using a natural vitamin E preparation to ensure absorption; simultaneously, 10 grams of astragalus polysaccharide is added, using astragalus polysaccharide powder with a purity of over 90%. The two fortifiers are evenly mixed into the basic forage and continued to be added until 15 days before harvest to enhance the antioxidant capacity of the cow's liver cells and reduce the interference of gallbladder inflammation on bezoar formation.

[0021] In a preferred embodiment, in step S8, when the feeding program has been implemented for 12 to 15 months, targeted stimulation feeding is stopped, and only the regular basic forage from the initial adaptation and conditioning phase is fed for 15 days to gently transition the cattle's metabolic state. Subsequently, the gallbladder is examined using ultrasound equipment. Once it is confirmed that the gallbladder contains at least 20 grams of bezoar, a professional veterinarian is arranged to perform a minimally invasive surgical extraction. The surgery involves making an incision of approximately 3 cm below the right costal arch, carefully removing the bezoar using specialized instruments to avoid rupture. Post-operatively, the incision is sutured and disinfected, and the cattle continue to be fed regular basic forage until they return to normal eating habits, ensuring their post-operative survival.

[0022] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are: 1. In this invention, by scientifically processing and selectively feeding bovine bile powder residue, suitable physiological conditions are created for the formation of bezoar. The active ingredients in the processed residue are more easily absorbed by cattle, gently regulating the gallbladder contraction rhythm and inducing bile stasis and cholesterol crystal precipitation, which is the core mechanism of bezoar formation. At the same time, the intermittent feeding rhythm ensures continuous stimulation without overburdening the cattle's hepatobiliary system, allowing bezoar to gradually form in a stable physiological environment, thereby fundamentally increasing bezoar production.

[0023] 2. This invention combines the dual advantages of resource recycling and livestock health protection. Previously discarded bovine bile powder residue is transformed into effective raw materials after processing, reducing breeding costs and waste emissions. Combined with liver-cleansing and bile-promoting basic forage, it stimulates bezoar production while maintaining liver and gallbladder health, reducing related diseases, and ensuring good growth of the herd, indirectly improving the stability of bezoar quality. The entire feeding process is closely integrated with daily breeding procedures, is simple to operate and easy to promote, and suitable for practical application by farmers. Attached Figure Description

[0024] Figure 1 This is a schematic diagram illustrating the overall process principle of the present invention; Figure 2 This is a schematic diagram of the step-by-step process of step S4 in this invention. Detailed Implementation

[0025] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0026] Reference Figure 1 A method for increasing bezoar production by feeding a specific combination of forage, the method comprising the following steps: S1: Select healthy local yellow cattle aged 3-5 years (preferably Qinchuan cattle, Luxi yellow cattle, etc.), weighing 400-500kg, with no history of liver and gallbladder diseases; collect tail vein blood to test bilirubin and bile acid levels, record initial weight, feeding habits and fecal characteristics, and establish individual files.

[0027] S2: Feed the animal with regular basic feed (60% corn silage + 30% alfalfa + 10% wheat bran) for 15 consecutive days, and supplement with 50g of multivitamin and mineral premixed feed per animal per day; adjust the feed moisture to about 60% to ensure optimal palatability.

[0028] S3: Prepare the basic herbal feed for liver cleansing and bile promotion: Chop dandelion, crush artemisia capillaris, and mix them evenly with other herbs. Each kilogram of herbal feed contains 15g of dandelion and 5g of artemisia capillaris.

[0029] S4: Add 50g of high-temperature sterilized and lactic acid bacteria fermented bovine bile powder residue (to remove toxic components) to the S3 basic forage 3 times a week with an interval of 2 days. The free bile acids in the fermented residue can regulate the gallbladder contraction rhythm and gently induce bile stasis and cholesterol crystal precipitation.

[0030] S5: Feed three times a day: feed hay containing S4 additive at 7:00 am and 6:00 pm (stimulation day), and feed pure S3 basic hay at 12:00 pm; each feeding amount is 2.5%-3% of the cattle's body weight, ensuring that there is no leftover feed.

[0031] S6: Record feeding speed and fecal consistency daily (target is formed soft stool); test bilirubin and alanine aminotransferase levels weekly: reduce the amount of Artemisia capillaris when feces are dry and hard, and suspend S4 stimulation feeding for 1-2 weeks when bilirubin is abnormal.

[0032] S7: After 6 months of feeding, add vitamin E (200 IU / head / day) + astragalus polysaccharide (10g / head / day) to the basic feed to enhance the antioxidant capacity of liver cells and reduce gallbladder inflammation.

[0033] S8: After feeding for 12-15 months, stop feeding S4 stimulation feed and feed only S2 basic feed for 15 days; after confirming the size of the bezoar (≥20g) by ultrasound, perform minimally invasive surgery to collect it; feed S2 feed after surgery to promote the recovery of cattle.

[0034] In step S1, select healthy local yellow cattle breeds aged 3 to 5 years, prioritizing Qinchuan or Luxi yellow cattle, with a weight between 400 and 500 kg and no history of liver or gallbladder disease. Collect tail vein blood samples from each cow and send them to a testing institution to determine bilirubin and bile acid levels, ensuring both indicators are within the normal range. Simultaneously record each cow's initial weight, daily feeding habits (including daily feeding frequency and duration of each feeding), and fecal characteristics (such as consistency and color) to establish an individual feeding record for each cow, allowing for targeted adjustments to the feeding program later.

[0035] In step S, cattle are fed a standard basic forage for 15 consecutive days. The forage formula consists of 60% corn silage, 30% alfalfa, and 10% wheat bran, with an additional 50 grams of multivitamin and mineral premix per head daily. The moisture content of the forage is adjusted to approximately 60%, which can be achieved by evenly spraying water to ensure optimal palatability. Feeding is divided into three portions daily, with each portion being 2% to 2.5% of the cattle's body weight. Observe the cattle's feeding behavior to ensure no leftover forage remains, thus stabilizing the cattle's gut microbiota and metabolic baseline.

[0036] In step S3, the basic forage includes: 50% corn silage, 25% alfalfa, 15% oat hay, 5% fresh dandelion, 3% dried artemisia capillaris, and 2% soybean meal.

[0037] In step S3, fresh dandelion is chopped into small pieces of about 1 cm, and dried artemisia capillaris is ground to 80 mesh. Then, all raw materials are put into a feed mixer and mixed for 10 minutes to ensure that all components are evenly mixed. Each kilogram of prepared feed should contain 15 grams of fresh dandelion and 5 grams of dried artemisia capillaris. The amount of soybean meal added must be strictly controlled to supplement sufficient protein to support the daily metabolic needs of cattle.

[0038] Step S4 specifically includes the following steps: S4-1: Place the collected bovine bile powder residue into a sterilization device at 121℃ for 30 minutes to thoroughly remove potentially toxic components. S4-2: Add a special bacterial culture to the sterilized bovine bile powder residue, ferment at a constant temperature of 37℃ for 48 hours, and control the pH value at around 4.5 to improve the absorption efficiency of free bile acids. S4-3: Select 3 days per week for stimulating feeding (with a 2-day interval between two consecutive days), and add 50 grams of fermented bovine bile residue to each cow daily. S4-4: Mix the fermented bovine bile powder residue evenly into the liver-cleansing and bile-promoting basic feed for the day, and feed it at 7 am and 6 pm. The effective ingredients gently regulate the gallbladder contraction rhythm, inducing bile stasis and cholesterol crystal precipitation.

[0039] In step S5, feed is given three times a day at fixed times: 7:00 AM, 12:00 PM, and 6:00 PM. On stimulation days (3 days a week), a liver-cleansing and bile-promoting basic feed supplemented with fermented bovine bile powder residue is given at 7:00 AM and 6:00 PM, and a pure liver-cleansing and bile-promoting basic feed is given at 12:00 PM. On non-stimulation days, pure liver-cleansing and bile-promoting basic feed is given all three times. The amount of feed given each time is calculated based on the cattle's weight, which is 2.5% to 3% of their body weight. For example, a 400 kg cattle is fed 10 to 12 kg of feed each time to ensure that the cattle can fully consume the feed and avoid waste.

[0040] In step S6, observe and record the feeding rate (time from the start of feeding to complete consumption) and fecal consistency (target: formed, soft stool) of each cow daily. Collect tail vein blood samples weekly to test bilirubin and alanine aminotransferase (ALT) levels. If the cow's feces are found to be hard and dry, reduce the proportion of dried Artemisia capillaris in the basal feed from 3% to 2%. If bilirubin levels are abnormally high, suspend targeted stimulation feeding for 1 to 2 weeks until the levels return to normal before resuming. If ALT levels are abnormal, reduce the amount of soybean meal added by 0.5% and increase the proportion of corn silage by 0.5%.

[0041] In step S7, after 6 months of continuous feeding of liver-cleansing and bile-promoting basic forage and targeted stimulation feeding, additional nutritional fortifiers are added to the basic forage. Each cow is supplemented with 200 IU of vitamin E daily, using a natural vitamin E preparation to ensure absorption; at the same time, 10 grams of astragalus polysaccharide is added, using astragalus polysaccharide powder with a purity of over 90%. The two fortifiers are evenly mixed into the basic forage and added continuously until 15 days before harvest to enhance the antioxidant capacity of the cow's liver cells and reduce the interference of gallbladder inflammation on the formation of bezoar.

[0042] In step S8, when the feeding program has been implemented for 12 to 15 months, targeted stimulation feeding is discontinued, and only the regular basic forage from the initial adaptation and conditioning phase is fed for 15 days to gently transition the cattle's metabolic state. Subsequently, the gallbladder is examined using ultrasound equipment. Once the gallbladder is confirmed to be at least 20 grams, a professional veterinarian performs a minimally invasive surgical extraction. A 3-centimeter incision is made below the right costal arch, and the gallbladder is carefully removed using specialized instruments, taking care to avoid rupture. Post-operatively, the incision is sutured and disinfected, and the cattle continue to be fed regular basic forage until they return to normal eating habits, ensuring their post-operative survival. Comparative example: (conventional method of adding untreated ox bile powder): Cattle selection: Same as the experimental group (30 healthy local yellow cattle aged 3-5 years, weighing 400-500kg, with no history of liver and gallbladder diseases), and files were established.

[0043] Basic feeding: Use conventional forage throughout the entire process (60% corn silage + 30% alfalfa + 10% wheat bran + 50g of multivitamin premix per head), without adding liver-cleansing and bile-promoting ingredients such as dandelion and artemisia capillaris.

[0044] Irritant treatment: The bovine bile powder residue is simply screened to remove impurities and chopped, without high-temperature sterilization or lactic acid bacteria fermentation.

[0045] Feeding method: Add 50g / head directly to the feed daily, twice a day, morning and evening (without interval stimulation rhythm), without pH adjustment or live bacteria testing.

[0046] Monitoring and Adjustment: Only feeding is recorded. Feeding is suspended when abnormalities are found. There is no regular bilirubin testing or precise dosage adjustment.

[0047] Bezoar collection: Collected from the same experimental group after 15 months, and the yield and quality were statistically analyzed.

[0048] Comparison table of effects between the examples and comparative examples:

[0049] In this embodiment, the specific forage combination method reduces the toxicity of bovine bile powder and enhances the activity of beneficial bacteria through high-temperature sterilization and targeted fermentation. The precise feeding rhythm and liver-cleansing and bile-promoting forage reduce the burden on the liver and gallbladder, which significantly increases the yield of bovine gallbladder (+85.8%) and the qualified rate (+53.4%), while reducing the risk of liver and gallbladder diseases in cattle (-23.3%). At the same time, it ensures feed stability and healthy survival, with clear and comprehensive beneficial effects.

[0050] From the above, we can conclude that: In this invention, by scientifically processing and selectively feeding bovine bile powder residue, suitable physiological conditions are created for the formation of bezoar. The active ingredients in the processed residue are more easily absorbed by cattle, gently regulating the gallbladder contraction rhythm and inducing bile stasis and cholesterol crystal precipitation, which is the core mechanism of bezoar formation. At the same time, the intermittent feeding rhythm ensures continuous stimulation without overburdening the cattle's hepatobiliary system, allowing bezoar to gradually form in a stable physiological environment, thereby fundamentally increasing bezoar production.

[0051] This invention offers the dual advantages of resource recycling and livestock health assurance. Previously discarded bovine bile powder residue is transformed into effective raw materials after processing, reducing breeding costs and waste emissions. Combined with liver-cleansing and bile-promoting basic forage, it stimulates bezoar production while maintaining liver and gallbladder health, reducing related diseases, and ensuring good growth of the herd, indirectly improving the stability of bezoar quality. The entire feeding process is closely integrated with daily breeding procedures, is simple to operate and easy to promote, and suitable for practical application by farmers.

[0052] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the term "comprising" or any other variations thereof is intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0053] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for increasing bezoar production through feeding a specific combination of forage, characterized in that: The method includes the following steps: S1: Select healthy local yellow cattle aged 3-5 years, weighing 400-500kg, with no history of liver and gallbladder diseases; then collect tail vein blood from these cattle to test bilirubin and bile acid levels, and record initial weight, feeding habits and fecal characteristics. S2: Feed regular basic forage for 15 consecutive days, supplementing with multivitamin and mineral premixed feed daily; adjust forage moisture to 60%; S3: Prepare the basic herbal feed for liver cleansing and bile promotion: Chop dandelion, crush artemisia capillaris, and mix them evenly with other herbs. Each kilogram of herbal feed contains 15g of dandelion and 5g of artemisia capillaris. S4: Add bovine bile powder residue that has been sterilized at high temperature and fermented with lactic acid bacteria to the basic forage 3 times a week with an interval of 2 days. S5: Feed three times a day: feed hay containing S4 additive at 7:00 AM and 6:00 PM, and feed pure S3 basic hay at 12:00 PM; each feeding amount is 2.5%-3% of the cattle's body weight; S6: Record feeding speed and stool consistency daily; test bilirubin and alanine aminotransferase levels weekly; S7: After 6 months of feeding, add vitamin E and astragalus polysaccharide to the basic forage; S8: After feeding for 12-15 months, stop feeding S4 stimulation feed and feed only S2 basic feed for 15 days; after confirming the size of the bezoar by ultrasound, perform minimally invasive surgery to collect it; feed S2 feed after surgery to promote the recovery of cattle.

2. The method for increasing bezoar production by feeding a specific combination of forage as described in claim 1, characterized in that: In step S1, healthy local yellow cattle aged 3 to 5 years are selected, with priority given to Qinchuan cattle or Luxi yellow cattle. The cattle should weigh between 400 and 500 kilograms and have no history of liver or gallbladder disease. Blood samples are collected from the tail vein of each cattle and sent to a testing institution to determine the levels of bilirubin and bile acids, ensuring that both indicators are within the normal range.

3. The method for increasing bezoar production by feeding a specific combination of forage as described in claim 1, characterized in that: In step S, cattle are fed conventional basic forage for 15 consecutive days. The forage formula consists of 60% corn silage, 30% alfalfa, and 10% wheat bran. Each head is supplemented with an additional 50 grams of multivitamin and mineral premix daily. The moisture content of the forage is adjusted to about 60%, which can be achieved by spraying water evenly to ensure that the palatability of the forage reaches the optimal state.

4. The method for increasing bezoar production by feeding a specific combination of forage as described in claim 1, characterized in that: In step S3, the basic forage includes: 50% corn silage, 25% alfalfa, 15% oat grass, 5% fresh dandelion, 3% dried artemisia capillaris, and 2% soybean meal.

5. The method for increasing bezoar production by feeding a specific combination of forages as described in claim 1, characterized in that: In step S3, fresh dandelion is chopped into small pieces of about 1 cm, and dried artemisia capillaris is crushed to 80 mesh size. Then all raw materials are put into a forage mixer and stirred for 10 minutes to ensure that all components are mixed evenly.

6. The method for increasing bezoar production by feeding a specific combination of forage as described in claim 1, characterized in that: Step S4 specifically includes the following steps: S4-1: Place the collected bovine bile powder residue into a sterilization device at 121℃ for 30 minutes. S4-2: Add a special bacterial culture to the sterilized bovine bile powder residue, place it in a constant temperature environment of 37℃ for 48 hours, and control the pH value at 4.5; S4-3: Select 3 days a week for stimulating feeding, and add 50 grams of fermented cow bile powder residue to each cow every day; S4-4: Mix the fermented bovine bile powder residue evenly into the liver-cleansing and bile-promoting basic feed for the day, and feed it at 7 am and 6 pm to induce bile stasis and cholesterol crystal precipitation.

7. The method for increasing bezoar production by feeding a specific combination of forages as described in claim 1, characterized in that: In step S5, the cattle are fed forage three times a day at 7:00 AM, 12:00 PM, and 6:00 PM. On stimulating days, the cattle are fed a liver-cleansing and bile-promoting basic feed with fermented bovine bile powder residue at 7:00 AM and 6:00 PM, and a pure liver-cleansing and bile-promoting basic feed at 12:00 PM. On non-stimulating days, the cattle are fed a pure liver-cleansing and bile-promoting basic feed three times a day. The amount of feed given each time is calculated based on the weight of the cattle and is 2.5% to 3% of their body weight.

8. The method for increasing bezoar production by feeding a specific combination of forage as described in claim 1, characterized in that: In step S6, the feeding speed and fecal consistency of each cow are observed and recorded daily; tail vein blood samples are collected weekly to test bilirubin and alanine aminotransferase (ALT) levels; if the cow's feces are found to be hard and dry, the proportion of dried Artemisia capillaris in the basic feed should be reduced from 3% to 2%; if the bilirubin level is abnormally high, targeted stimulation feeding should be suspended for 1 to 2 weeks until the level returns to normal before resuming; if the ALT level is abnormal, the amount of soybean meal added should be reduced by 0.5%, and the proportion of silage corn should be increased by 0.5%.

9. The method for increasing bezoar production by feeding a specific combination of forage as described in claim 1, characterized in that: In step S7, after 6 months of continuous feeding of liver-cleansing and bile-promoting basic forage and targeted stimulation feeding, additional nutritional fortifiers are added to the basic forage; each cow is supplemented with 200 IU of vitamin E daily, using natural vitamin E preparations to ensure absorption; at the same time, 10 grams of astragalus polysaccharide is added, using astragalus polysaccharide powder with a purity of over 90%.

10. The method for increasing bezoar production by feeding a specific combination of forage as described in claim 1, characterized in that: In step S8, when the feeding program is implemented for 12 to 15 months, the targeted stimulation feeding is stopped, and only the regular basic forage of the early adaptation and conditioning stage is fed for 15 days to gently transition the cattle's metabolic state; then, the cattle's gallbladder is examined using B-ultrasound equipment, and after confirming that the gallbladder is more than 20 grams, a professional veterinarian is arranged to perform minimally invasive surgery to collect the gallbladder.

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