Leak-proof double-insurance chloroform fumigation device for analyzing carbon, nitrogen and phosphorus in microbial biomass

Abstract

The invention relates to a leakproof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen and phosphorus analysis, and belongs to the technical field of microbiological analysis experiment equipment.The leakproof double-insurance chloroform fumigation device comprises a fumigation box and a sealing cover, an annular inserting groove is formed in the bottom face of the sealing cover, and a first sealing gasket is embedded in the annular inserting groove; a lifting assembly used for driving the sealing cover to vertically ascend and descend is arranged outside the fumigation box. An annular frame extending horizontally is fixedly arranged on the inner wall of the fumigation box, the annular frame and the fumigation box are integrally formed, and a second sealing gasket is fixedly arranged on the lower surface of the annular frame; a turnover sealing plate is arranged below the annular frame body, and the edge of one side of the sealing plate is hinged to the inner wall of the fumigation box; an overturning assembly used for driving the sealing plate to rotate is further arranged in the fumigation box; a placement frame for bearing samples is horizontally arranged in the fumigation box, a moving assembly for driving the placement frame to move in the vertical direction is further arranged in the fumigation box, and the device has the effect of improving the detection accuracy of carbon, nitrogen and phosphorus in the microbial biomass.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of microbial analysis experimental equipment, in particular to a leakage-proof double-insurance chloroform fumigation device for microbial carbon, nitrogen and phosphorus analysis. BACKGROUND

[0002] Microbial carbon, nitrogen and phosphorus analysis is mainly used for evaluating the total biomass of microbial communities in soil, sediment or water bodies and the content of carbon, nitrogen and phosphorus elements, and is an important index for reflecting the microbial activity, nutrient cycling capacity and environmental quality of an ecological system. The analysis can provide key data support for soil health evaluation, agricultural nutrient management, ecological restoration effect monitoring and environmental pollution assessment.

[0003] The existing device is usually composed of a fumigation barrel, a chloroform adding dish and a sealing cover. The fumigation barrel is internally provided with a sample rack, the sealing cover is internally provided with a sealing gasket, and the upper end of the fumigation barrel is inserted and adapted with the sealing cover. The working principle is based on physical fumigation: the dish containing a quantitative liquid chloroform is placed on the sample rack together with the soil sample, the sealing cover is manually closed, the sealing gasket and the upper end of the fumigation barrel are tightly abutted, the opening end of the fumigation barrel is sealed, the chloroform is naturally volatilized into steam at room temperature, diffuses and penetrates the sample, kills the microorganisms and lyses the cells. After fumigation for a specific time, the container is ventilated, the remaining chloroform is removed, and the fumigated and non-fumigated control samples are extracted and chemically analyzed, the microbial carbon, nitrogen and phosphorus are calculated by the difference between the nutrient contents of the two. The whole process mainly relies on passive volatilization and natural diffusion, and the operation is relatively simple.

[0004] According to the related art, the sealing gasket is easily thinned in thickness due to long-time extrusion after being used for a period of time. After the sealing cover is closed, the upper end of the fumigation barrel is difficult to tightly abut against the sealing gasket, so that the sealing is not tight. The chloroform steam is easily leaked from the joint gap, so that it is difficult to maintain an effective and stable fumigation concentration in the fumigation barrel, and even the laboratory environment and the control sample can be polluted due to leakage. Therefore, there is a defect that the detection accuracy of microbial carbon, nitrogen and phosphorus is low. SUMMARY

[0005] In order to improve the detection accuracy of microbial carbon, nitrogen and phosphorus, the application provides a leakage-proof double-insurance chloroform fumigation device for microbial carbon, nitrogen and phosphorus analysis.

[0006] The leakage-proof double-insurance chloroform fumigation device for microbial carbon, nitrogen and phosphorus analysis provided by the application adopts the following technical scheme: A leak-proof, double-protection chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis includes a fumigation chamber and a sealing cover. The bottom surface of the sealing cover has an annular insertion groove, within which a first sealing gasket is embedded. The top opening edge of the fumigation chamber is configured as an insertion portion that can be inserted into the annular insertion groove. A lifting assembly is provided on the outside of the fumigation chamber for driving the sealing cover to move vertically. A horizontally extending annular frame is fixed to the inner wall of the fumigation chamber, integrally formed with the chamber. A second sealing gasket is fixed to the lower surface of the annular frame. A flip-up sealing plate is located below the annular frame, with one edge hinged to the inner wall of the fumigation chamber. A flipping assembly is also provided inside the fumigation chamber for driving the sealing plate to rotate around a hinge axis. A sample-carrying rack is horizontally arranged inside the fumigation chamber, and a moving assembly is also provided inside the chamber for driving the rack to move vertically.

[0007] By adopting the above technical solution, the top of the fumigation chamber achieves a first seal through a sealing cap and a connecting part, while the interior achieves a second seal through a flip-up sealing plate, an annular frame, and a second sealing gasket, forming a "double-insurance" leak-proof structure. The lifting assembly controls the opening and closing of the sealing cap, the flipping assembly controls the opening and closing of the sealing plate, and the moving assembly adjusts the sample position, achieving a fully enclosed fumigation process. This effectively prevents chloroform leakage, improves the stability of the fumigation concentration, and thus enhances the accuracy of microbial biomass carbon, nitrogen, and phosphorus detection.

[0008] Optionally, a support plate is fixedly provided on the outer bottom of the fumigation box; the lifting assembly includes a vertically arranged threaded sleeve and a screw that is threadedly engaged with the threaded sleeve; the bottom end of the threaded sleeve is rotatably connected to the support plate; a connecting plate is fixedly connected to the upper end of the screw, and the end of the connecting plate away from the screw is fixedly connected to the upper surface of the sealing cover; a telescopic rod is also fixedly provided between the connecting plate and the support plate, and the telescopic rod is composed of multiple sections of rod body that are slidably sleeved together in sequence.

[0009] By adopting the above technical solution, the screw is driven to rise and fall by rotating the threaded sleeve, which drives the sealing cover to open and close smoothly; the telescopic rod provides guidance and support to ensure that the sealing cover remains horizontal during the rising and falling process, and avoids deviation that could lead to poor sealing.

[0010] Optionally, a first drive motor is fixedly mounted on the support plate, and a first gear is fixedly mounted on the output shaft of the first drive motor; a second gear is fixedly sleeved on the outer periphery of the threaded sleeve, and the first gear meshes with the second gear for transmission.

[0011] By adopting the above technical solution, the first drive motor drives the threaded sleeve to rotate through gear transmission, realizing the electric control of the sealing cover. The operation is convenient and the sealing force is uniform, which is conducive to maintaining the sealing effect for a long time.

[0012] Optionally, a first guide rail is vertically fixed on each of the opposite side walls inside the fumigation chamber, and a first slide rod is slidably connected in each of the first guide rails; a hinge rod is rotatably connected between the two first slide rods, the hinge rod is located at the upper end of the first slide rod, and the hinge rod passes through the sealing plate and is fixedly connected to the sealing plate.

[0013] By adopting the above technical solution, the first guide rail provides vertical guidance for the first slide rod, ensuring its smooth lifting and lowering; the first slide rod provides stable support for the hinge rod, thereby enabling the sealing plate to reliably flip and accurately position under the drive of the hinge rod, thus completing the opening and closing action of the sealing plate.

[0014] Optionally, a second drive motor is provided at one end of the hinge rod, and the second drive motor is fixedly connected to the adjacent first slide rod; the flipping assembly includes a third gear and a fourth gear, the third gear is fixed on the output shaft of the second drive motor, and the fourth gear is fixedly sleeved on the end of the hinge rod, and the third gear meshes with the fourth gear.

[0015] By adopting the above technical solution, the second drive motor drives the hinge rod to rotate through gear transmission, thereby controlling the flipping of the sealing plate and realizing the automatic opening and closing of the internal second seal, improving operating efficiency and sealing reliability.

[0016] Optionally, a partition is horizontally fixed at the bottom of the fumigation chamber, and the first guide rail and the placement rack are both located above the partition. Second guide rails are vertically fixed at opposite ends of the upper surface of the partition, and a second slide rod is slidably connected in each second guide rail. A push plate for pushing the sealing plate upward is fixed on the upper side wall of the second slide rod, and the push plate is horizontally arranged. A pushing component for driving the corresponding second slide rod to move upward is provided at each second guide rail.

[0017] By adopting the above technical solution, the pusher plate can push the sealing plate upward when needed to assist it in closing completely or to provide additional clamping force, thereby further enhancing the reliability of the second seal and preventing poor sealing due to the weight of the sealing plate or the influence of airflow.

[0018] Optionally, a pressure plate is horizontally arranged above the partition, and the placement rack is located above the pressure plate; the pushing assembly includes a first push rod and a second push rod, one end of the first push rod is hinged to the lower surface of the pressure plate, and the other end is hinged to a slider, the slider being slidably connected to the partition; one end of the second push rod is hinged to the slider, and the other end is hinged to a corresponding second slide rod.

[0019] By adopting the above technical solution, when the placement frame descends, the pressure plate also descends. Through the linkage of the first push rod, the slider and the second push rod, the second slide rod and the push plate are driven to move upward, thereby achieving auxiliary pressing and sealing of the sealing plate. The structure is compact and the linkage is reliable.

[0020] Optionally, the moving component includes a drive cylinder and a moving rod; the drive cylinder is vertically fixed to the bottom of the fumigation chamber, and its output shaft is fixedly connected to one end of the moving rod; the moving rod is vertically arranged, and its upper end passes through the partition and is fixedly connected to the placement frame.

[0021] By adopting the above technical solution, the electric cylinder drives the placement rack to rise and fall smoothly through the moving rod, which facilitates the placement and removal of samples and realizes automated operation.

[0022] Optionally, a pressure sensor is installed on the upper surface of the push plate to detect the pressure it receives and output a pressure signal; the output end of the pressure sensor is connected to a comparator, which compares the pressure signal with a preset reference value and outputs an execution signal; the drive electric cylinder is connected to the output end of the comparator and operates in response to the execution signal.

[0023] By adopting the above technical solution, the pressure sensor monitors the pressing force of the push plate on the sealing plate in real time. When the pressure is insufficient, the comparator outputs a signal to drive the electric cylinder to adjust the position of the placement frame to enhance the pressing and sealing, thereby realizing intelligent feedback and adjustment of the sealing state.

[0024] Optionally, the first and second sealing gaskets are made of fluororubber.

[0025] By adopting the above technical solution, fluororubber has good resistance to chloroform corrosion, high elasticity and aging resistance, and can maintain good sealing effect even after long-term use, further improving the leakage prevention capability and service life of the device.

[0026] In summary, this application includes at least one of the following beneficial technical effects: 1. The double-sealing structure of the sealing cover and sealing plate effectively prevents chloroform leakage, maintains a stable chloroform concentration in the fumigation chamber, and improves the fumigation effect and detection accuracy of microorganisms; 2. It adopts electric lifting, tilting and moving components to realize automated control of sealing opening and closing and sample positioning, which is convenient to operate and reliable in sealing; 3. A pressure sensor and feedback control system are installed to enable real-time monitoring and intelligent adjustment of the sealing status, further improving sealing reliability and the intelligence level of the device. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of a leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen and phosphorus analysis according to an embodiment of this application; Figure 2 This is a structural cross-sectional view in the embodiments of this application to illustrate the price increase / decrease combination; Figure 3This is a cross-sectional view of the structure of the flipping component in the embodiments of this application; Figure 4 This is a system guide diagram illustrating the starting of the drive electric cylinder in the embodiments of this application.

[0028] In the diagram, 1. Fumigation chamber; 11. Annular frame; 12. Second sealing gasket; 13. Sealing plate; 14. Placement rack; 15. First guide rail; 151. First slide rod; 152. Hinge rod; 16. Second drive motor; 17. Partition plate; 18. Second guide rail; 181. Second slide rod; 182. Push plate; 19. Pressure plate; 2. Sealing cover; 21. Annular insertion groove; 22. First sealing gasket; 3. Lifting assembly; 31. Threaded sleeve; 32. Screw; 4. Tilting assembly; 41. Third gear; 42. Fourth gear; 5. Moving assembly; 51. Drive cylinder; 52. Moving rod; 6. Support plate; 61. Connecting plate; 62. Telescopic rod; 63. First drive motor; 64. First gear; 65. Second gear; 7. Pushing assembly; 71. First push rod; 712. Slider; 72. Second push rod; 8. Pressure sensor. Detailed Implementation

[0029] The following is in conjunction with the appendix Figures 1-4 This application will be described in further detail.

[0030] This application discloses a leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen and phosphorus analysis.

[0031] refer to Figure 1 A leak-proof, double-protection chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis includes a fumigation chamber 1 and a sealing cover 2. The fumigation chamber 1 is a vertical chamber structure made of stainless steel or corrosion-resistant engineering plastic. The sealing cover 2 is a square cover that covers the top opening of the fumigation chamber 1, and the two are sized to match. A support plate 6 is horizontally fixed to the bottom of one side of the fumigation chamber 1. A lifting assembly 3 for driving the sealing cover 2 to rise and fall is provided on the support plate 6.

[0032] The lifting assembly 3 drives the sealing cover 2 to move smoothly up and down in the vertical direction, realizing the opening and closing of the fumigation box 1. During the lifting process, the sealing cover 2 can be precisely aligned and form a reliable seal with the top of the fumigation box 1.

[0033] refer to Figure 1The lifting assembly 3 includes a vertically arranged threaded sleeve 31 and a screw 32 threadedly engaged with the threaded sleeve 31. The threaded sleeve 31 is a tubular structure with internal threads, and its bottom end is rotatably connected to the support plate 6 via a bearing, ensuring that the threaded sleeve 31 can rotate smoothly around its own axis. A horizontally arranged connecting plate 61 is fixedly connected to the upper end of the screw 32. The end of the connecting plate 61 away from the screw 32 is fixedly connected to the upper surface of the sealing cover 2. When the threaded sleeve 31 rotates, the screw 32 and the sealing cover 2 are driven to rise and fall vertically through threaded transmission. A telescopic rod 62 is also fixed between the connecting plate 61 and the support plate 6. The telescopic rod 62 is composed of multiple rod sections that slide and fit together sequentially.

[0034] A first drive motor 63 is fixedly mounted on the support plate 6. The first drive motor 63 is a servo motor, which features high control precision and stable operation. A first gear 64 is fixedly mounted on the output shaft of the first drive motor 63, and a second gear 65 is fixedly mounted on the outer circumference of the threaded sleeve 31. The first gear 64 and the second gear 65 mesh and transmit power, forming a gear transmission mechanism to realize the transmission of power.

[0035] When the sealing cover 2 needs to be closed, the first drive motor 63 starts, and the output shaft drives the first gear 64 to rotate. The first gear 64 drives the second gear 65 and the threaded sleeve 31 to rotate synchronously through meshing. The threaded sleeve 31 and the screw 32 have threaded transmission, which drives the screw 32, the connecting plate 61 and the sealing cover 2 to move slowly downward. The telescopic rod 62 retracts synchronously, always providing a stable guide for the sealing cover 2, ensuring that the sealing cover 2 remains in a horizontal state and falls accurately until it is sealed and fitted with the top of the fumigation box 1. When it needs to be opened, the first drive motor 63 rotates in the opposite direction, driving the sealing cover 2 to rise smoothly upward. The telescopic rod 62 extends accordingly. The whole process is convenient to operate and runs smoothly.

[0036] refer to Figure 1 , Figure 2 and Figure 3 The bottom surface of the sealing cover 2 has an annular insertion groove 21, which is a groove structure surrounding the edge of the sealing cover 2, and a first sealing gasket 22 is embedded therein. The top opening edge of the fumigation chamber 1 is constructed as an insertion part that can be inserted into the annular insertion groove 21. The first sealing gasket 22 is made of fluororubber, which has excellent resistance to chloroform corrosion, high elasticity and aging resistance. Even under long-term compression, it can still maintain a good sealing effect and effectively prevent chloroform vapor from leaking from the top interface.

[0037] After the operator starts the device, the sealing cover 2 moves slowly downward under the drive of the lifting component 3. The insertion part at the top of the fumigation box 1 gradually approaches the annular insertion groove 21 and finally inserts precisely into the groove, forming a tight squeeze and fit with the first sealing gasket 22. The first sealing line is quickly built, providing initial sealing protection for the subsequent fumigation process.

[0038] refer to Figure 1 , Figure 2 and Figure 3 The inner wall of the fumigation chamber 1 is fixedly provided with a horizontally extending annular frame 11. The annular frame 11 is integrally formed with the fumigation chamber 1 and is made of the same stainless steel material, which has high structural strength and is not easily deformed. A second sealing gasket 12 is fixedly provided on the lower surface of the annular frame 11. The second sealing gasket 12 is also made of fluororubber material, forming a double sealing guarantee with the first sealing gasket 22.

[0039] Below the annular frame 11 is a reversible sealing plate 13, which is a square plate adapted to the internal cross-section of the fumigation chamber 1. First guide rails 15 are vertically fixed on opposite side walls inside the fumigation chamber 1. Each first guide rail 15 has a groove-shaped structure, and a first slide rod 151 is slidably connected within each first guide rail 15, allowing it to slide vertically along the first guide rail 15. A hinge rod 152 is rotatably connected between the two first slide rods 151. The hinge rod 152 is located at the upper end of the first slide rod 151 and passes through the sealing plate 13, being fixedly connected to it and causing the sealing plate 13 to rotate synchronously. A second drive motor 16, also a servo motor, is provided at one end of the hinge rod 152 and is fixedly connected to the adjacent first slide rod 151. A flipping assembly 4, capable of flipping the sealing plate 13, is provided at the second drive motor 16.

[0040] The flipping assembly 4 includes a third gear 41 and a fourth gear 42. The third gear 41 is fixed on the output shaft of the second drive motor 16, and the fourth gear 42 is fixedly sleeved on the end of the hinge rod 152. The third gear 41 and the fourth gear 42 mesh to form a gear transmission mechanism, which provides power for the flipping of the sealing plate 13.

[0041] When the second seal needs to be closed, the second drive motor 16 starts, and the output shaft drives the third gear 41 to rotate. The third gear 41 drives the fourth gear 42 and the hinge rod 152 to rotate through meshing. The hinge rod 152 drives the sealing plate 13 to flip upward around the hinge axis. The first slide rod 151 slides upward synchronously in the first guide rail 15 to provide stable support for the hinge rod 152 until the upper surface of the sealing plate 13 is tightly attached to the second sealing gasket 12, completing the second seal. When it needs to be opened, the second drive motor 16 rotates in the opposite direction, the sealing plate 13 flips downward to open, and the first slide rod 151 slides down along the first guide rail 15 to reset. The opening and closing control of the double sealing structure is precise and reliable.

[0042] refer to Figure 1 , Figure 2 and Figure 3A partition 17 is horizontally fixed inside the fumigation chamber 1, dividing the interior of the fumigation chamber 1 into upper and lower layers. Above the partition 17, a sample placement rack 14 is horizontally arranged. The placement rack 14 is a square frame structure made of stainless steel, with evenly distributed ventilation holes on its surface to facilitate the uniform diffusion of chloroform vapor. A first guide rail 15 is located above the partition 17. Second guide rails 18 are vertically fixed at opposite ends of the upper surface of the partition 17. The second guide rails 18 are also groove-shaped structures. A second slide rod 181 is slidably connected within each second guide rail 18, and the second slide rod 181 can slide vertically along the second guide rail 18. A push plate 182 is fixed to the upper side wall of the second slide rod 181 for pushing the sealing plate 13 upward. The push plate 182 is horizontally arranged, and its upper surface is adapted to the lower surface of the sealing plate 13.

[0043] A pressure plate 19 is horizontally positioned above the partition 17, and a placement rack 14 is located above the pressure plate 19. The pressure plate 19 is a square plate adapted to the interior of the fumigation chamber 1. Each second guide rail 18 is equipped with a pushing assembly 7 for driving the corresponding second slide bar 181 upward. The pushing assembly 7 includes a first push rod 71 and a second push rod 72. One end of the first push rod 71 is hinged to the lower surface of the pressure plate 19, and the other end is hinged to a slider 712. The slider 712 is slidably connected to the partition 17 and can move horizontally along the partition 17. One end of the second push rod 72 is hinged to the slider 712, and the other end is hinged to the corresponding second slide bar 181, forming a linkage transmission mechanism.

[0044] As the placement rack 14 descends, it gradually moves downward, bringing it into contact with the pressure plate 19. This causes the pressure plate 19 to move downwards synchronously. The pressure plate 19 then pushes the first push rod 71 downwards through the hinge point. The first push rod 71 drives the slider 712 to slide along the partition 17 towards the second guide rail 18. The slider 712 then pushes the second push rod 72 upwards through the hinge point. The second push rod 72 drives the second slide rod 181 to slide upwards along the second guide rail 18. The push plate 182 then moves upwards, pushing the upper surface of the sealing plate 13 to fit tightly against the second sealing gasket 12, further enhancing the pressure of the second seal and ensuring the sealing effect. When the placement rack 14 rises, the second slide rod 181 and the push plate 182 move downwards due to gravity, and push the pressure plate 19 upwards through the first push rod 71 and the second push rod 72, releasing the pushing effect on the sealing plate 13.

[0045] refer to Figure 2 , Figure 3 and Figure 4The fumigation chamber 1 is also equipped with a moving assembly 5 that drives the placement frame 14 to move vertically. The moving assembly 5 includes a drive cylinder 51 and a moving rod 52. The drive cylinder 51 is vertically fixed to the bottom of the fumigation chamber 1. It is a high-precision cylinder with high control accuracy and stable thrust. Its output shaft is fixedly connected to one end of the moving rod 52. The moving rod 52 is vertically set, with its upper end passing through the partition 17 and fixedly connected to the placement frame 14, driving the placement frame 14 to rise and fall synchronously.

[0046] A pressure sensor 8 is mounted on the upper surface of the push plate 182 to detect the pressure it receives and output a pressure signal. A comparator is connected to the output of the pressure sensor 8. The comparator compares the pressure signal with a preset reference value and outputs an execution signal. The drive cylinder 51 is connected to the output of the comparator and operates in response to the execution signal, forming a closed-loop control system.

[0047] When samples need to be placed or removed, the drive cylinder 51 is activated, and the output shaft drives the moving rod 52 and the placement rack 14 to move upward. The placement rack 14 rises to a height that is easy to operate, and the operator can place the sample on the placement rack 14 or remove the sample from the placement rack 14. After the sample is placed, the drive cylinder 51 drives the placement rack 14 to move downward until it reaches the preset fumigation position. At this time, the pressure sensor 8 on the push plate 182 detects the pressure signal of the sealing plate 13 and transmits the signal to the comparator. The comparator compares the detected signal with the preset reference value. If the pressure is insufficient, the output execution signal controls the drive cylinder 51 to continue to drive the placement rack 14 to descend. The push assembly 7 enhances the pushing force of the push plate 182 on the sealing plate 13 until the pressure reaches the preset value. The drive cylinder 51 then stops to ensure reliable sealing.

[0048] The implementation principle of the leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis in this application embodiment is as follows: In use, firstly, the lifting assembly 3 drives the sealing cover 2 to rise, opening the fumigation chamber 1, and placing the container containing the sample and chloroform on the placement rack 14; then, the first drive motor 63 starts, driving the sealing cover 2 to descend, causing the top insertion part of the fumigation chamber 1 to insert into the annular insertion groove 21, forming a first seal with the first sealing gasket 22; next, the drive cylinder 51 drives the placement rack 14 to descend to a preset position, while simultaneously driving the push assembly 7 to move the push plate 182. The sealing plate 13 is pushed up, and the second drive motor 16 starts to drive the sealing plate 13 to flip, forming a second seal with the second sealing gasket 12 on the lower surface of the annular frame 11. The pressure sensor 8 monitors the sealing pressure in real time and adjusts it through feedback from the comparator to ensure the reliability of the double seal. Chloroform naturally evaporates to form vapor, which fumigates the sample. After fumigation, the drive cylinder 51 drives the placement rack 14 to rise, pushing the component 7 to reset. The second drive motor 16 drives the sealing plate 13 to flip and open, and the first drive motor 63 drives the sealing cover 2 to rise, allowing the sample to be removed for subsequent analysis. The entire process effectively prevents chloroform leakage through the double sealing structure, maintains a stable fumigation concentration, and significantly improves the accuracy of microbial biomass carbon, nitrogen, and phosphorus detection.

[0049] The embodiments described in this specific implementation are preferred embodiments of this application and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A leak-proof double-protection chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis, comprising a fumigation chamber (1) and a sealing cover (2), characterized in that: The bottom surface of the sealing cover (2) is provided with an annular insertion groove (21), and a first sealing gasket (22) is embedded in the annular insertion groove (21); the top opening edge of the fumigation box (1) is constructed as an insertion part that can be inserted into the annular insertion groove (21); the outside of the fumigation box (1) is provided with a lifting assembly (3) for driving the sealing cover (2) to rise and fall vertically; the inner wall of the fumigation box (1) is fixed with a horizontally extending annular frame (11), the annular frame (11) is integrally formed with the fumigation box (1), and the annular frame (11) A second sealing gasket (12) is fixedly provided on the lower surface of the annular frame (11); a flip-out sealing plate (13) is provided below the annular frame (11), and one side edge of the sealing plate (13) is hinged to the inner wall of the fumigation box (1); a flipping assembly (4) for driving the sealing plate (13) to rotate around the hinge axis is also provided in the fumigation box (1); a placement rack (14) for carrying samples is horizontally provided in the fumigation box (1), and a moving assembly (5) for driving the placement rack (14) to move in the vertical direction is also provided in the fumigation box (1).

2. The leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis according to claim 1, characterized in that: A support plate (6) is fixedly provided on the outer side of the bottom of the fumigation box (1); the lifting assembly (3) includes a vertically arranged threaded sleeve (31) and a screw (32) threadedly engaged with the threaded sleeve (31); the bottom end of the threaded sleeve (31) is rotatably connected to the support plate (6); a connecting plate (61) is fixedly connected to the upper end of the screw (32), and the end of the connecting plate (61) away from the screw (32) is fixedly connected to the upper surface of the sealing cover (2); a telescopic rod (62) is also fixedly provided between the connecting plate (61) and the support plate (6), and the telescopic rod (62) is composed of multiple sections of rod body that are slidably sleeved together in sequence.

3. The leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis according to claim 2, characterized in that: A first drive motor (63) is fixedly installed on the support plate (6), and a first gear (64) is fixedly installed on the output shaft of the first drive motor (63); a second gear (65) is fixedly sleeved on the outer periphery of the threaded sleeve (31), and the first gear (64) and the second gear (65) mesh and transmit power.

4. The leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis according to claim 1, characterized in that: The fumigation box (1) has two vertically fixed first guide rails (15) on opposite side walls inside. Each first guide rail (15) has a first slide rod (151) slidably connected inside. A hinge rod (152) is rotatably connected between the two first slide rods (151). The hinge rod (152) is located at the upper end of the first slide rod (151) and passes through the sealing plate (13) and is fixedly connected to the sealing plate (13).

5. A leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis according to claim 4, characterized in that: A second drive motor (16) is provided at one end of the hinge rod (152), and the second drive motor (16) is fixedly connected to the adjacent first slide rod (151); the flipping assembly (4) includes a third gear (41) and a fourth gear (42), the third gear (41) is fixed on the output shaft of the second drive motor (16), and the fourth gear (42) is fixedly sleeved on the end of the hinge rod (152), and the third gear (41) and the fourth gear (42) mesh.

6. A leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis according to claim 4, characterized in that: A partition (17) is horizontally fixed at the bottom of the fumigation box (1). The first guide rail (15) and the placement rack (14) are both located above the partition (17). A second guide rail (18) is vertically fixed at each of the opposite ends of the upper surface of the partition (17). A second slide rod (181) is slidably connected in each second guide rail (18). A push plate (182) for pushing the sealing plate (13) upward is fixed on the upper side wall of the second slide rod (181). The push plate (182) is horizontally set. A pushing component (7) for driving the corresponding second slide rod (181) to move upward is provided at each second guide rail (18).

7. A leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis according to claim 6, characterized in that: A pressure plate (19) is horizontally arranged above the partition (17), and the placement rack (14) is located above the pressure plate (19); the pushing assembly (7) includes a first push rod (71) and a second push rod (72), one end of the first push rod (71) is hinged to the lower surface of the pressure plate (19), and the other end is hinged to a slider (712), the slider (712) is slidably connected to the partition (17); one end of the second push rod (72) is hinged to the slider (712), and the other end is hinged to the corresponding second slide rod (181).

8. A leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis according to claim 7, characterized in that: The moving component (5) includes a drive cylinder (51) and a moving rod (52); the drive cylinder (51) is vertically fixed to the bottom of the fumigation box (1), and its output shaft is fixedly connected to one end of the moving rod (52); the moving rod (52) is vertically arranged, and the upper end of the moving rod (52) passes through the partition (17) and is fixedly connected to the placement rack (14).

9. A leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis according to claim 8, characterized in that: A pressure sensor (8) is installed on the upper surface of the push plate (182) to detect the pressure it receives and output a pressure signal; the output end of the pressure sensor (8) is connected to a comparator, which is used to compare the pressure signal with a preset reference value and output an execution signal; the drive electric cylinder (51) is connected to the output end of the comparator and is used to act in response to the execution signal.

10. A leak-proof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen, and phosphorus analysis according to claim 9, characterized in that: The first sealing gasket (22) and the second sealing gasket (12) are made of fluororubber.

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