Slide plate and dyeing box combination for realizing independent dyeing method of each sample piece

Abstract

The invention discloses a slide plate and dyeing box combination for realizing an independent dyeing method of each sample piece, and relates to a sample piece and dyeing box technology in the field of biomedicine. The sample piece is made of mold opening hard plastic, the elastic piece and the rigid reinforcing supporting plate are made of spring steel metal, the mold opening hard plastic guarantees the overall high hardness and structural stability of the sample piece, the spring steel elastic piece has the excellent elastic recovery capacity, and the repeated clamping and fixing effect of the sample piece is guaranteed. The staining box is provided with a liquid inlet and outlet circulating pipeline which is communicated with a reagent barrel and a waste liquid barrel, the middle of the staining box is divided into ten independent small clamping grooves, the ten clamping grooves correspond to ten sample pieces, and small-test-dosage pollution-free isolated independent staining can be carried out. Compared with a traditional method that a large amount of reagents are manually poured into a staining jar at a time, a sample piece is hung in a hanging basket and repeatedly soaked for about 30 days, the staining quality can be obviously improved, especially the batch-to-batch stability is good, and full automation can be conveniently achieved through the combination of the sample piece and the staining box.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, specifically to a combination of a glass slide and a staining box for achieving an independent staining method for each sample slide. Background Technology

[0002] Existing glass slides of the same type are mostly made of all-plastic material and molded in one piece. However, the application scenarios of glass slides require that the whole slide has high hardness and is not easily deformed. At the same time, the spring clips used to fix the sample slides need to have good flexibility and elasticity in order to achieve stable clamping and convenient handling of the sample slides.

[0003] However, it is difficult to find moldable plastic materials that combine high hardness and high elasticity, making it impossible for existing glass slides to meet both performance requirements simultaneously. More importantly, when the glass slides are filled with sample pieces and placed in an oven at 65-80℃ for baking, and then cooled to room temperature to remove the sample pieces, the elastic clamping force of the all-plastic glass slides will lose its resilience due to high-temperature aging, and may have to be scrapped after a single use.

[0004] Traditional staining methods involve immersing multiple slides in a basket within a large staining tank. Reagents require manual emptying and replacement, and are typically reused repeatedly over extended periods. This leads to issues such as reagent aging and uneven concentration between different batches of slides, resulting in significant batch-to-batch variability. Furthermore, the gap between the basket frame and the slides allows for the adhesion of large amounts of reagent. When transferring to the next reagent tank, this adhering reagent is carried into the new reagent, and detached cell debris may also adhere to another slide, causing cross-contamination between reagents and samples, thus affecting staining quality and stability.

[0005] To address the aforementioned problems, the inventors proposed a combination of a glass slide and a staining box that enables independent staining of each sample slide to solve these issues. Summary of the Invention

[0006] To address the limitations of adaptability in the combination of glass slides and staining boxes, the present invention aims to provide a combination of glass slides and staining boxes that enables independent staining of each sample slide.

[0007] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: a glass slide plate for realizing an independent staining method for each sample slide, comprising a glass slide plate and a plurality of sample slides, wherein the glass slide plate is provided with a limiting structure for accommodating and limiting each of the sample slides; Each of the sample pieces is inserted into one of the limiting structures; The glass slide is also provided with a spring piece that cooperates with each of the limiting structures. The spring piece is used to elastically press and fix the sample piece that is inserted into the limiting structure. It also includes a rigid reinforcing support plate disposed on the side of the glass slide along its length direction; The glass slide is made of a single piece of hard plastic, and the spring is made of a metal material with elastic recovery capability.

[0008] Preferably, the integral hard plastic material of the glass slide includes molded hard plastic, the metal material of the spring sheet with elastic recovery capability includes spring steel, and the rigid reinforcing support plate is made of stainless steel.

[0009] Preferably, the glass slide includes a plate body and inserts installed at both ends of the plate body. Multiple magnetic blocks are provided on the inserts and the outer wall of the plate body. The bottom surface of the gap between the insert and the plate body is an arc surface. A round rod is fixedly connected to the side of the insert away from the plate body.

[0010] Preferably, the limiting structure includes a plurality of limiting portions disposed on the glass slide, the plurality of limiting portions surrounding to form a bearing area for accommodating the sample piece, the plurality of limiting portions forming an opening on one side of the bearing area, the sample piece being inserted into the bearing area through the opening, and the plurality of limiting portions partially constraining the sample piece accommodated in the bearing area from at least three directions.

[0011] Preferably, the plurality of limiting portions include at least a first stop, a second stop, a third stop, a fourth stop, and a fifth stop arranged around the edge of the sample piece; In the insertion direction of the sample piece, there are a first stop and a second stop, which are used to block the sample piece after it is inserted into place; The glass slide has a third stop, a fourth stop, and a fifth stop on two sides perpendicular to the insertion direction. The fourth stop and the fifth stop are located at different positions on the same side, and the third stop, the fourth stop, and the fifth stop form gaps with the glass slide. When the sample is inserted into place, the two edges of the sample are respectively accommodated in the gaps. It also includes a first guide block and a second guide block respectively disposed on the same side as the third block and the fourth block, for providing guidance for the insertion of the sample sheet.

[0012] Preferably, a clamping groove is formed between the first guide block and the second guide block of the adjacent limiting structure at intervals, and an insertion hole communicating with the clamping groove is opened on the glass slide. The spring is located in the clamping groove, and the spring has a plate fixing foot, which is inserted into the insertion hole; The bearing area is provided with a clearance groove, and at least a portion of the spring piece is located in the clearance groove to facilitate the insertion of the sample piece: for clearance when inserting it at an angle along one side of the sample piece's length direction; The upper part of the bearing area is provided with a pass-through opening for easy picking up and putting down of sample pieces; the surface of the bearing area is provided with grooves to increase the friction with the sample pieces.

[0013] Preferably, a staining box for implementing an independent staining method for each sample slide includes a staining box in which the glass slide and the sample slide are inserted and supported inside the staining box; The staining box has several independent slots inside, and several sample pieces are respectively inserted into several independent slots. Several partitions are fixedly connected to the inner wall of the staining box, and each independent slot is formed by two partitions and the inner wall of the staining box. The dyeing box has a liquid inlet structure on its top outer wall and a liquid outlet structure at its bottom. The liquid inlet structure and the liquid outlet structure extend into independent small tanks and are connected. A liquid level sensor is installed on the dyeing box.

[0014] Preferably, the liquid inlet structure includes a drain hole and a drain insertion hole. The drain hole is located on the bottom outer wall of the dyeing box and extends into the interior of the dyeing box to form a channel. The drain insertion hole is located at the bottom of the independent slot and communicates with the channel of the drain hole.

[0015] Preferably, the liquid inlet structure includes a liquid inlet hole, and the inner wall of the independent card slot is provided with a liquid inlet port, which is connected to the liquid inlet hole. The dyeing box is provided with an interface for connecting a liquid level sensor connector, and the liquid level sensor is installed on the outer wall of the transparent tube connected to the interface of the liquid level sensor connector.

[0016] Preferably, the staining box has U-shaped support openings on both sides of its walls for supporting the inserted glass slides.

[0017] Preferably, a concave groove is provided on the outer wall of the dyeing box, and a heating plate is installed on the concave groove. Compared with the prior art, the beneficial effects of the present invention are as follows: 1. The sample sheet of this invention uses a molded hard plastic glass slide, a spring steel spring sheet, and a stainless steel rigid reinforcing support plate. The molded hard plastic ensures the high hardness and structural stability of the sample sheet as a whole. The spring steel spring sheet has excellent elastic recovery ability. Even after being baked at 65-80℃ and cooled to room temperature, it can still quickly rebound, ensuring the repeated clamping and fixing effect of the sample sheet. This solves the problem that traditional plastic spring sheets are scrapped after a single use. The rigid reinforcing support plate is attached to the side of the glass slide, which can effectively counteract the deformation that may occur in the plastic material, further ensuring the flatness of the sample sheet for long-term use and significantly extending its service life.

[0018] 2. The staining box of this invention adopts a partitioned structure, with 10 independent small slots formed by partitions inside. Each independent small slot corresponds to the staining space of one sample slide, so that each sample slide is in an independent staining environment, avoiding the problem of cross-contamination between reagent rooms and cell samples in traditional basket-type staining. At the same time, the staining box is equipped with independent liquid inlet and outlet structures, which can realize the automatic circulation supply of fresh small doses of reagents for each staining, replacing the traditional mode of long-term reuse of reagents in large staining tanks. This ensures that each sample slide is stained in reagents with uniform concentration and fresh components, effectively eliminating batch-to-batch differences and improving the consistency and accuracy of staining results. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0021] Figure 2 This is a schematic diagram of the assembly of the staining box and sample slides of the present invention.

[0022] Figure 3 For the present invention Figure 2 A schematic diagram of the structure at point A in the middle.

[0023] Figure 4 This is a schematic diagram of the top structure of the dyeing box of the present invention.

[0024] Figure 5 For the present invention Figure 4 A schematic diagram of the structure at point B.

[0025] Figure 6 This is a schematic diagram of the spring structure of the present invention.

[0026] Figure 7 This is a schematic diagram of the spring sheet structure with an upper retaining fold edge according to the present invention.

[0027] Figure 8 This is a schematic diagram of the glass slide and sample sheet structure of the present invention.

[0028] Figure 9 This is a schematic diagram of the dyeing box structure of the present invention.

[0029] Figure 10 For the present invention Figure 9 A schematic diagram of the structure at point C.

[0030] Figure 11 This is a schematic diagram of the glass slide of the present invention.

[0031] Figure 12 For the present invention Figure 11 A schematic diagram of the structure at point D.

[0032] Figure 13 This is a schematic diagram of the structure of the glass slide, sample sheet, and rigid reinforcing support plate on the outer wall of the glass slide of the present invention.

[0033] In the diagram: 1. Staining box; 11. Concave groove; 12. Liquid inlet; 13. Interface for connecting liquid level sensor connector; 14. Drain hole; 15. Liquid inlet; 16. Independent slot; 17. Drain insertion hole; 18. Partition; 2. Glass slide; 20. Plate body; 21. Insert holder; 22. Magnetic block; 23. Round rod; 3. Sample piece; 4. Spring piece; 41. Insert fixing foot; 5. Limiting structure; 51. Limiting part; 511 511. First stop block; 512. Second stop block; 513. Third stop block; 514. Fourth stop block; 515. Fifth stop block; 516. First guide block; 517. Second guide block; 518. Grip groove; 52. Bearing area; 53. Opening; 54. Avoidance groove; 55. Take-out and placement avoidance passage; 56. Slot; 6. Rigid reinforcing support plate; 7. Insertion hole; 8. U-shaped support opening; 9. Extended side panel; 10. Upper stop folded edge. Detailed Implementation

[0034] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Example

[0035] like Figure 1 - Figure 13 As shown, the present invention provides a glass slide plate for realizing an independent staining method for each sample slide, including a glass slide plate 2 and a plurality of sample slides 3, wherein the glass slide plate 2 is provided with a limiting structure 5 for accommodating and limiting each sample slide 3; Each sample piece 3 is inserted into a limiting structure 5; The glass slide 2 is also provided with a spring piece 4 that cooperates with each limiting structure 5. The spring piece 4 is used to elastically press and fix the sample piece 3 that is inserted into the limiting structure 5. Specifically, the sample slide 3 is divided into a label part and a cell part. The label part is fixed to the glass slide 2 by the limiting structure 5. In actual use, the cell part is suspended below the glass slide 2 and can be directly immersed in the test solution tank. The label part of the sample slide 3 and the glass slide 2 do not come into contact with the test solution, which can reduce mucus and minimize cross-contamination. In practical use, multiple sample slides 3 can be laid flat on the glass slide plate 2 at one time. At the same time, it can facilitate the transportation and positioning of automated equipment. The glass slide plate 2 can be moved to the sealing, baking, microscope scanning, loading and unloading stations. Since the sample slides 3 are laid flat on the glass slide plate 2, sealing, baking and scanning can be performed directly on the glass slide plate 2 without the need to transfer sample slides, which greatly improves work efficiency.

[0036] It also includes a rigid reinforcing support plate 6 disposed on the side of the glass slide 2 along the length direction of the glass slide 2; The glass slide 2 is made of one-piece hard plastic, while the spring 4 is made of metal with elastic recovery capability.

[0037] The glass slide 2 is made of one-piece hard plastic material, including molded hard plastic; the spring sheet 4 is made of metal material with elastic recovery ability, including spring steel; and the rigid reinforcing support plate 6 is made of stainless steel. The purpose of this setup is that after the glass slide 2 filled with sample slide 3 is baked at a low temperature in the oven, the spring steel spring 4 can still rebound, and the rigid reinforcing support plate 6 is attached to the side of the glass slide 2, located on the outside of the sample slide 3, combined with... Figure 13 Even if the plastic material is deformed, it can be flattened without deformation. When the sample piece 3 is mounted on the glass slide plate 2, the spring piece 4 elastically engages it, making it easy to put, take out or suspend the sample piece 3.

[0038] The glass slide 2 includes a plate body 20 and a bracket 21 installed at both ends of the plate body 20. Multiple magnetic blocks 22 are provided on the bracket 21 and the outer wall of the plate body 20. The bottom surface of the gap between the bracket 21 and the plate body 20 is set as an arc surface. A round rod 23 is fixedly connected to the side of the bracket 21 away from the plate body 20. Among them, the side of the insert 21 that is off from the plate 20 is set with a beveled edge, and the two sides of the adjacent beveled edge are set with beveled edges in a prismatic shape. The beveled edge and prismatic shape formed by the beveled edge on the insert 21 can facilitate guidance and positioning, and the matching efficiency is high. The purpose of this arrangement is that multiple magnetic blocks 22 are used to magnetically position the glass slide 2 and then lift and transport it horizontally. The bottom surface of the gap between the insert 21 and the plate 20 is set as an arc surface, which is used to lock onto the top two ends of the staining box 1 for positioning and support, so as to facilitate the positioning of the glass slide 2 on the staining box. The round rod 23 is on the outer side of both ends of the glass slide 2, which is beneficial for hanging and transporting the glass slide 2 during fully automated processing. It can also be used to support the sample glass slide 2 to be placed flat on the table and pulled forward by the hook. Specifically, it can be mounted on a fully automated transport platform, moving back and forth between various workstations such as slide baking, reagent tanks, slide sealing, loading and unloading, and microscope slide reading and scanning, which is convenient, fast, and greatly improves work efficiency.

[0039] The specific method for moving glass slide 2 can be selected according to the actual situation. For example, hooks can be used to hook the round rods 23 on both sides for translation; supports can also be used to transfer the slide on the arc surface; or a magnetic suction plate robot can be used to suction multiple magnetic blocks 22 on the glass slide 2 for horizontal lifting.

[0040] The limiting structure 5 includes a plurality of limiting parts 51 disposed on the glass slide 2. The plurality of limiting parts 51 surround to form a bearing area 52 for accommodating the sample sheet 3. The plurality of limiting parts 51 form an opening 53 on one side of the bearing area 52. The sample sheet 3 is inserted into the bearing area 52 through the opening 53. The plurality of limiting parts 51 partially constrain the sample sheet 3 contained in the bearing area 52 from at least three directions. The purpose of this arrangement is that the sample piece 3 is inserted into the bearing area 52 along the opening 53, and multiple limiting parts 51 limit the sample piece 3 in the bearing area 52 to prevent the sample piece 3 from shifting or shaking inside the bearing area 52.

[0041] The plurality of limiting parts 51 include at least a first stop 511, a second stop 512, a third stop 513, a fourth stop 514 and a fifth stop 515 arranged around the edge of the sample piece 3; In the insertion direction of the sample piece 3, there is a first stop 511 and a second stop 512, which are used to block the sample piece 3 after it is inserted into place. The first block 511 and the second block 512 are located on the top surface of the plate 20 in the glass slide 2, and are used for top alignment when the sample slide 3 is installed. The glass slide 2 is provided with a third stop 513, a fourth stop 514 and a fifth stop 515 on both sides perpendicular to the insertion direction. The fourth stop 514 and the fifth stop 515 are located at different positions on the same side, and a gap is formed between the third stop 513, the fourth stop 514 and the fifth stop 515 and the glass slide 2. When the sample slide 3 is inserted into place, the two sides of the sample slide 3 are respectively accommodated in the gap. The third stop 513, the fourth stop 514 and the fifth stop 515 are used to limit the offset of the sample piece 3 on both sides, and the gap design is adapted to the sample piece 3 to achieve seamless positioning and fixation. It also includes a first guide block 516 and a second guide block 517 respectively disposed on the same side as the third stop block 513 and the fourth stop block 514, for providing guidance for the insertion of the sample piece 3; The purpose of this arrangement is that, guided by the first guide block 516 and the second guide block 517, the sample slide 3 is inserted along the opening 53 formed on one side of the bearing area 52. The third stop block 513, the fourth stop block 514 and the fifth stop block 515 form gaps with the glass slide 2. During the insertion and sliding process, the two sides of the sample slide 3 are respectively accommodated in the gaps. When the sample slide 3 is inserted into the bearing area 52 of the glass slide 2, the first stop block 511 and the second stop block 512 on the glass slide 2 block it and limit its movement, thereby completing the insertion operation of the sample slide 3 into the bearing area 52 of the glass slide 2.

[0042] Specifically, the magnetic block 22 is positioned on the plate 20 between the third stop 513 and the fourth stop 514 in the adjacent limiting structure 5, which facilitates operation and positioning without affecting clamping. Example 2: Based on Example 1, a spring sheet 4 structure with a insert plate fixing foot 41 is provided; A clamping groove 518 is formed between the first guide block 516 and the second guide block 517 of the adjacent limiting structure at intervals, and an insertion hole 7 connected to the clamping groove 518 is opened on the glass slide 2. The spring piece 4 is located in the clamping groove. The spring piece 4 has a plate fixing foot 41, and the plate fixing foot 41 is inserted into the insertion hole 7. The bearing area 52 is provided with a clearance groove 54, and at least a part of the spring piece 4 is located in the clearance groove 54 for clearance when the sample piece 3 is inserted at an angle. The spring piece 4 provides elastic fixation for the sample piece 3 in the bearing area 52. The purpose of this arrangement is that three sockets 7 are provided, and the three sockets 7 are equally spaced on the inner side of the clamping groove 518, combined with Figure 6 As shown, the spring piece 4 is inserted into the three corresponding insertion holes 7 in the clamping groove 518 through three insert plate fixing feet 41. At least a part of the spring piece 4 is located at the avoidance groove 54. During the process of inserting the sample piece 3 into the bearing area 52, the spring piece 4 at the avoidance groove 54 is squeezed. The elasticity of the spring piece 4 elastically fixes one side of the sample piece 3. The spring sheet 4 has two types. The first type is: the spring sheet 4 is located in the avoidance groove 54 area and has an extended side stop 9 on the side facing the glass slide plate 2, which is used to prevent the sample sheet from being squeezed out from the side when it is under force. The upper part of the bearing area 52 is provided with a pick-up and put-out clearance opening 55, and the surface of the bearing area 52 is provided with grooves 56 for increasing the friction with the sample sheet 3; The pick-and-place clearance opening 55 can be set in an arc shape. The first stop 511 and the second stop 512 are set near the arc trajectory of the pick-and-place clearance opening 55. This facilitates pick-and-place while ensuring that the sample slide 3 and the glass slide plate 2 have a large contact area, improving clamping stability. At the same time, the bearing area 52 is close to the groove 56 on the outer wall of the sample slide 3, generating mutual friction with the sample slide 3, which plays an anti-slip role for the sample slide 3 and further strengthens the suspension effect of the glass slide plate 2 on the sample slide 3. Combination Figure 7 As shown, the insert plate fixing feet 41 of the spring piece 4 are set as two, which are equally spaced and inserted into the corresponding two insertion holes 7 in the clamping groove 518. At least a part of the spring piece 4 is located at the avoidance groove 54. This part is provided with a local height bend near the opening 53 of the bearing area 52. The remaining unbent part has the same height as the thickness of the sample piece 3. During the process of inserting the sample piece 3 into the bearing area 52, the spring piece 4 at the avoidance groove 54 is squeezed. The elasticity of the spring piece 4 elastically fixes one side of the sample piece 3. The second type of spring sheet 4 is: In addition to the spring sheet 4 having an extended side baffle 9 on the side that is in the avoidance groove 54 area and is close to the glass slide 2, the spring sheet 4 has an upper baffle fold 10 on the side that is away from the first guide block 516, which is used to block the sample sheet 3 so that it does not fall upward. The spring clip, which is partially bent, consists of four parts. Figure 7 ,and Figure 8 As shown in the diagram, the rightmost sample piece 3 is installed in the bearing area 52 by a bent spring piece 4. During installation, the thickness of the sample piece 3 is the same as the remaining unbent part, which is used for the sample piece 3 to pass through. The bent part is attached to one side surface of the sample piece 3 to provide auxiliary limiting for the sample piece 3 and prevent the sample piece 3 from falling out from the surface of the sample piece 3 after it is installed in the glass slide plate 2.

[0043] Example 3: Based on Examples 1 and 2, a staining box for implementing an independent staining method for each sample slide is provided and used in conjunction with a glass slide for implementing an independent staining method for each sample slide. Place the glass slide into the staining box, including staining box 1, and insert glass slide 2 and sample slide 3 into the inside of staining box 1; The staining box 1 has several independent slots 16 inside, and several sample slides 3 are inserted into several independent slots 16 respectively. The two sides of the independent slots 16 are inclined to guide the slides 2. Several partitions 18 are fixedly connected to the inner wall of the staining box 1. The independent slots 16 are formed by two partitions 18 and the inner wall of the staining box 1. The several independent slots 16 allow each sample slide 3 to enter and exit the liquid independently, preventing cross-contamination between sample slides 3, and the combined installation also saves space. A liquid inlet structure is provided on the top outer wall of the dyeing box 1, and a liquid outlet structure is provided at the bottom of the dyeing box 1. The liquid inlet structure and the liquid outlet structure extend into the interior of an independent small tank and are connected. A liquid level sensor is installed on the dyeing box 1. The staining solution enters the interior of the independent card slot 16 through the inlet structure to stain the sample slide 3, and then flows out through the outlet structure. The liquid level sensor detects the liquid level height and controls the liquid level, which facilitates the automatic control of the inlet and outlet of the liquid. The traditional large staining tank has been replaced by the independent card slot 16, which can automatically extract a small amount of fresh reagent each time for automatic circulation, ensuring that each sample slide 3 is fresh reagent.

[0044] The liquid inlet structure includes a drain hole 14 and a drain insertion hole 17. The drain hole 14 is opened on the bottom outer wall of the staining box 1 and extends into the interior of the staining box 1 to form a channel. The drain insertion hole 17 is opened at the bottom of the independent slot 16 and communicates with the channel of the drain hole 14. The purpose of this design is to allow the dyeing solution inside the independent card slot 16 to be discharged through the drain hole 17 to the drain hole 14.

[0045] The liquid inlet structure includes a liquid inlet hole 12, and an inlet 15 is provided on the inner wall of the independent card slot 16. The inlet 15 is connected to the liquid inlet hole 12. The dyeing box 1 is provided with an interface 13 for connecting to the liquid level sensor connector. The liquid level sensor is installed on the transparent tube connected to the interface 13 of the liquid level sensor connector. The purpose of this setup is to allow the staining solution to enter through the inlet hole 12 and then through the inlet port 15 into the interior of the independent card slot 16, so as to independently stain the sample slide 3.

[0046] A concave groove 11 is provided on the outer wall of the staining box 1, and a heating plate is installed on the concave groove 11 to assist in heating the staining solution and accelerate the staining reaction.

[0047] The workflow of this invention is as follows: Step one: First, insert the spring 4 into the slot 518 of the glass slide 2. The spring 4 is configured with two different usage structures, combined with... Figure 7 and Figure 8 As shown, springs 4 with different shapes and structures are selected according to the installation requirements of sample sheet 3 to the bearing area 52 in glass slide 2 under different conditions; Step two: Insert sample piece 3 from one side opening 53 of the bearing area 52 in the glass slide 2, aligning the top edge of the label with the inner side of the first stop 511. Then, slide sample piece 3 with one long side angled towards the clearance groove 54 of the glass slide 2, inserting it along the inner side of the bearing area 52. During insertion, press the spring piece 4 at the clearance groove 54, then press the other long side downwards, and use the elasticity of the spring piece 4 to elastically fix one side of sample piece 3. Next, push the sample piece forward until it is inserted, allowing sample piece 3 to engage with the first stop 511 and the second stop. When 512 is touched, the first block 511 and the second block 512 block the sample slide 3, while the long sides on both sides enter the gaps of the third block 513, the fourth block 514 and the fifth block 515 respectively, thereby completing the insertion and fixation of the sample slide 3 in the bearing area 52 of the glass slide plate 2. At the same time, the bearing area 52 is close to the groove 56 on the outer wall of the sample slide 3, and generates mutual friction with the sample slide 3, which plays an anti-slip role for the sample slide 3 and further strengthens the suspension effect of the glass slide plate 2 on the sample slide 3. The same method is used to fill the other bearing areas 52 with sample slides 3. Step 3: Using the round rods 23 at both ends of the top of the glass slide 2, the glass slide 2 filled with sample slides 3 is lifted and moved into the staining box 1. The glass slide 2 and sample slides 3 are aligned with the top port of the staining box 1, and the bottom end of each sample slide 3 needs to be aligned. The independent slot 16 corresponding to each sample slide 3 is located inside the staining box 1. Then, the slide is slowly lowered. The bottom surface of the gap between the insert 21 and the plate 20 is set with an arc surface, and it is locked at both ends of the top of the staining box 1. Thus, the glass slide 2 and sample slides 3 are inserted into the inside of the staining box 1. The staining box 1 and glass slide 2 are respectively set with multiple sets for side-by-side use, forming a multi-row production line for staining, which is highly efficient. At the same time, multiple rows of glass slide 2 can be hung and installed inside multiple rows of staining boxes 1 at the same time. Only slow calibration of the insertion is required between them. Step four: The staining solution enters through the inlet 12 and then through the inlet 15 into the independent slot 16 to independently stain the sample slide 3. After staining, the staining solution inside the independent slot 16 flows through the drain hole 17 to the drain hole 14 for discharge. During this process, two level sensors on the transparent tube connected to the interface 13 of the level sensor connector detect the liquid level, thus facilitating the control of the inlet and outlet liquid levels. A heating plate is installed on the concave groove 11 to provide auxiliary heating for the staining solution and accelerate the staining reaction. Furthermore, the inlet and outlet of the staining box 1 are connected to the pump valve, reagent tank, and waste liquid tank, and the round rod 23 of the glass slide 2 is connected to the hook lifting and moving motor structure, which can easily achieve full automation.

[0048] Obviously, those skilled in the art can make various modifications and variations to this invention without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this invention and their equivalents, this invention also intends to include these modifications and variations.

Claims

1. A glass slide for implementing an independent staining method for each sample slide, comprising a glass slide (2) and multiple sample slides (3), characterized in that: The glass slide (2) is provided with a limiting structure (5) for accommodating and limiting each of the sample pieces (3); Each of the sample pieces (3) is inserted into one of the limiting structures (5); The glass slide (2) is also provided with a spring piece (4) that cooperates with each of the limiting structures (5). The spring piece (4) is used to elastically press and fix the sample piece (3) that is inserted into the limiting structure (5). It also includes a rigid reinforcing support plate (6) disposed on the side of the glass slide (2) along the length direction of the glass slide (2); The glass slide (2) is made of one-piece hard plastic material, and the spring sheet (4) is made of metal material with elastic recovery capability.

2. The glass slide for implementing an independent staining method for each sample slide as described in claim 1, characterized in that, The glass slide (2) is made of one-piece hard plastic material including molded hard plastic, the spring sheet (4) is made of metal material with elastic recovery capability including spring steel, and the rigid reinforcing support plate (6) is made of stainless steel.

3. The glass slide for implementing an independent staining method for each sample slide as described in claim 1, characterized in that, The glass slide (2) includes a plate body (20) and a bracket (21) installed at both ends of the plate body (20). Multiple magnetic blocks (22) are provided on the outer wall of the bracket (21) and the plate body (20). The bottom surface of the gap between the bracket (21) and the plate body (20) is set as an arc surface. A round rod (23) is fixedly connected to the side of the bracket (21) away from the plate body (20).

4. A glass slide for implementing an independent staining method for each sample slide as described in any one of claims 1-3, characterized in that, The limiting structure (5) includes a plurality of limiting parts (51) disposed on the glass slide (2). The plurality of limiting parts (51) surround to form a bearing area (52) for accommodating the sample piece (3). The plurality of limiting parts (51) form an opening (53) on one side of the bearing area (52). The sample piece (3) is inserted into the bearing area (52) through the opening (53). The plurality of limiting parts (51) partially constrain the sample piece (3) accommodated in the bearing area (52) from at least three directions.

5. The glass slide for implementing an independent staining method for each sample slide as described in claim 4, characterized in that, The plurality of limiting parts (51) include at least a first stop (511), a second stop (512), a third stop (513), a fourth stop (514) and a fifth stop (515) arranged around the edge of the sample piece (3). In the insertion direction of the sample piece (3), there is a first stop (511) and a second stop (512) to block the sample piece (3) after it is inserted into place; The glass slide (2) is provided with a third stop (513), a fourth stop (514) and a fifth stop (515) on both sides perpendicular to the insertion direction. The fourth stop (514) and the fifth stop (515) are located at different positions on the same side, and a gap is formed between the third stop (513), the fourth stop (514) and the fifth stop (515) and the glass slide (2). When the sample piece (3) is inserted into place, the two sides of the sample piece (3) are respectively accommodated in the gap. It also includes the outer side of the first guide block (516) and the second guide block (517) respectively disposed on the same side as the third block (513) and the fourth block (514), for providing guidance for the insertion of the sample piece (3).

6. The glass slide for implementing an independent staining method for each sample slide as described in claim 4, characterized in that, A clamping groove (518) is formed between the first guide block (516) and the second guide block (517) of the adjacent limiting structure (5) at intervals, and an insertion hole (7) communicating with the clamping groove (518) is opened on the glass plate (2). The spring piece (4) is located in the clamping groove (518), the spring piece (4) has a plate fixing foot (41), and the plate fixing foot (41) is inserted into the insertion hole (7); The bearing area (52) is provided with a clearance groove (54), and at least a portion of the spring piece (4) is located at the clearance groove (54) for clearance when the sample piece (3) is inserted at an angle. The upper part of the bearing area (52) is provided with a pick-up and put-out clearance opening (55), and the surface of the bearing area (52) is provided with grooves (56) for increasing the friction with the sample piece (3).

7. A staining box for implementing an independent staining method for each sample slide, applied to a glass slide for implementing an independent staining method for each sample slide according to any one of claims 1-6, characterized in that, Includes a staining box (1), into which the glass slide (2) and sample slide (3) are inserted; The staining box (1) has several independent slots (16) inside, and several sample pieces (3) are respectively inserted into several independent slots (16). Several partitions (18) are fixedly connected to the inner wall of the staining box (1). The independent slots (16) are formed by two partitions (18) and the inner wall of the staining box (1). The dyeing box (1) has a liquid inlet structure on its top outer wall and a liquid drain structure at its bottom. The liquid inlet structure and the liquid drain structure extend into the interior of an independent small tank and are connected. A liquid level sensor is installed on the dyeing box (1).

8. The staining box for implementing an independent staining method for each sample slide as described in claim 7, characterized in that, The liquid inlet structure includes a drain hole (14) and a drain insertion hole (17). The drain hole (14) is opened on the bottom outer wall of the staining box (1) and extends into the interior of the staining box (1) to form a channel. The drain insertion hole (17) is opened at the bottom of the independent card slot (16) and communicates with the channel of the drain hole (14).

9. A staining box for implementing an independent staining method for each sample slide as described in claim 7, characterized in that, The liquid inlet structure includes a liquid inlet hole (12), and a liquid inlet (15) is provided on the inner wall of the independent card slot (16). The liquid inlet (15) is connected to the liquid inlet hole (12). The dyeing box (1) is provided with an interface (13) for connecting a liquid level sensor connector. The liquid level sensor is installed on the outer wall of the transparent tube connected to the interface (13) of the liquid level sensor connector.

10. A staining box for implementing an independent staining method for each sample slide as described in claim 7, characterized in that, The staining box (1) has U-shaped support openings (8) on both sides for supporting the inserted glass slide (2), and a concave groove (11) is provided on the outer wall of the staining box (1), and a heating plate is installed on the concave groove (11).

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