Control method of cell culture workstation and cell culture workstation

Abstract

The invention relates to the technical field of biomedical treatment, and particularly provides a control method of a cell culture workstation and the cell culture workstation. Specifically, the cell culture workstation comprises a culture device, a circulating pipe and a driving device, a liquid inlet and a liquid outlet of the culture device are communicated with the circulating pipe, and the circulating pipe is used for guiding a culture solution to circularly flow; the driving device is connected with the incubator and can drive the incubator to rotate; the control method comprises the steps that whether bubbles exist in a circulating pipe communicating with the liquid outlet or not is detected; and selectively driving the incubator to rotate according to a detection result so as to discharge air in the incubator. According to the cell culture workstation, the driving device is arranged to be connected with the culture device, and bubble detection is carried out in the cell culture process, so that when air is retained in the culture device, the culture device can be driven to rotate through the driving device, and the air in the culture device is discharged; therefore, the culture effect and success rate of the cell culture workstation are improved.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, specifically providing a control method for a cell culture workstation and a cell culture workstation. Background Technology

[0002] Cell culture refers to all in vitro culture, which means taking tissues from a living animal, separating the tissues into single cells through mechanical or enzymatic digestion, and then incubating and culturing them under specific in vivo conditions that simulate the in vivo physiological environment, so that they can survive and grow.

[0003] Current cell culture methods generally involve seeding cells into a culture vessel, supplying nutrient solution to the vessel via a circulation tube, and then placing the vessel in an incubator for cultivation. However, during the nutrient solution supply process, air may become trapped within the culture vessel. If air is trapped, some cells may not come into contact with the culture medium, leading to poor culture results and culture failure.

[0004] Therefore, a new technical solution is needed in this field to solve the above problems. Summary of the Invention

[0005] The present invention aims to solve the above-mentioned technical problems, namely, to solve the problem that poor culture effect and culture failure caused by air retention in existing culturers.

[0006] In a first aspect, the present invention provides a control method for a cell culture workstation, the cell culture workstation comprising a culture vessel, a circulation tube, and a drive device, wherein the culture vessel is capable of storing and culturing cells, the culture vessel has an inlet and an outlet, both of which are connected to the circulation tube, and the culture medium flows within the circulation tube; the drive device is connected to the culture vessel and is capable of driving the culture vessel to rotate; the control method comprises: detecting whether there are air bubbles in the circulation tube connected to the outlet; and selectively driving the culture vessel to rotate based on the detection result to expel air from the culture vessel.

[0007] In the preferred embodiment of the control method for the cell culture workstation described above, the step of "selectively driving the culture vessel to rotate according to the detection results" specifically includes: when air bubbles are detected and the cumulative time of air bubbles appearing within the detection period is greater than a first preset time, the culture vessel is driven to rotate.

[0008] In a preferred embodiment of the control method for the cell culture workstation described above, the control method further includes: if the cumulative time for the appearance of bubbles is greater than a second preset time during the detection cycle, then the rotation speed of the culture device is reduced; wherein the second preset time is greater than the first preset time.

[0009] In a preferred embodiment of the control method for the cell culture workstation described above, the control method further includes: if the cumulative time for the appearance of bubbles within the detection cycle is greater than a third preset time, then the cell culture workstation issues an alarm; wherein the third preset time is greater than the second preset time.

[0010] In the preferred embodiment of the control method for the cell culture workstation described above, the step of "driving the culture vessel to rotate" specifically includes: driving the culture vessel to reciprocate.

[0011] In a preferred embodiment of the control method for the cell culture workstation described above, the driving device includes a drive motor and a clamping assembly. The drive motor is connected to the clamping assembly and is capable of driving the clamping assembly to rotate. The clamping assembly is used to clamp the culture vessel; and / or the rotation axis of the culture vessel extends horizontally and is perpendicular to the axis of the culture vessel.

[0012] In the preferred embodiment of the control method for the cell culture workstation described above, the clamping assembly includes a first clamp, a second clamp, and a connecting shaft. The first clamp is fixedly connected to one end of the connecting shaft or integrally formed therewith. The other end of the connecting shaft is fixedly connected to the drive shaft of the drive motor. One end of the first clamp is pivotally connected to one end of the second clamp. The other end of the first clamp is provided with a first connecting structure, and the other end of the second clamp is provided with a second connecting structure. The first connecting structure and the second connecting structure are detachably fixedly connected. The culture vessel is clamped between the first clamp and the second clamp.

[0013] In a preferred embodiment of the control method for the cell culture workstation described above, the clamping assembly further includes a clamping member. The first or second clamp is provided with a threaded hole, and the clamping member is provided with a thread that matches the threaded hole. The clamping member is installed in the threaded hole, and one end of the clamping member can pass through the threaded hole and abut against the outer wall of the culture vessel; and / or the connecting shaft is horizontally arranged and perpendicular to the axis of the culture vessel.

[0014] In a preferred embodiment of the control method for the cell culture workstation described above, the culture vessel includes a shell and a plurality of hollow fiber tubes installed inside the shell. The interior of the hollow fiber tubes forms channels for the flow of culture medium, and the cells are attached to the outer wall of the hollow fiber tubes.

[0015] In a second aspect, the present invention also provides a cell culture workstation, the cell culture workstation including a controller configured to perform the control method described above.

[0016] In the case of adopting the above technical solution, the cell culture workstation of the present invention is connected to the culture vessel by setting a driving device and performing bubble detection during the cell culture process. When there is air trapped in the culture vessel, the driving device can drive the culture vessel to rotate to expel the air in the culture vessel, thereby improving the culture effect and success rate of the cell culture workstation.

[0017] Furthermore, the cell culture workstation of the present invention, by setting a detection cycle, only activates the drive device to drive the culture vessel to rotate when the cumulative time of bubble appearance within the detection cycle is greater than a first preset time, which can avoid misjudgment and improve the accuracy of control.

[0018] Furthermore, the cell culture workstation of the present invention reduces the rotation speed of the culture vessel when the cumulative time for the appearance of air bubbles is greater than a second preset time, which is more conducive to the expulsion of air from the culture vessel.

[0019] Furthermore, the cell culture workstation of the present invention issues an alarm when the cumulative time for the appearance of air bubbles exceeds a third preset time, thus promptly reminding staff to carry out maintenance when pipeline failure occurs.

[0020] Furthermore, the cell culture workstation of the present invention, by causing the culture vessel to rotate back and forth, is more conducive to expelling air from the culture vessel.

[0021] Furthermore, the cell culture workstation of the present invention uses a clamping component to hold and fix the culture vessel, which facilitates operation and provides a better user experience.

[0022] Furthermore, the cell culture workstation of the present invention, by setting a clamping member to abut against the culture vessel, can prevent the culture vessel from falling out between the first clamp and the second clamp, thereby improving the reliability of the clamping assembly. Attached Figure Description

[0023] The preferred embodiments of the present invention are described below with reference to the accompanying drawings, in which: Figure 1 This is a schematic diagram of the cell culture workstation of the present invention. Figure 1 ; Figure 2 This is a schematic diagram of the cell culture workstation of the present invention. Figure 2 ; Figure 3 This is a schematic diagram of the structure of the culture chamber of the cell culture workstation of the present invention; Figure 4 This is a schematic diagram of the structure of the culture vessel and gripper assembly of the cell culture workstation of the present invention; Figure 5 This is a schematic diagram of the mounting component of the cell culture workstation of the present invention. Figure 1 ; Figure 6 This is a schematic diagram of the mounting component of the cell culture workstation of the present invention. Figure 2 ; Figure 7 This is a schematic diagram of the structure of the first mounting component of the cell culture workstation of the present invention; Figure 8 This is a schematic diagram of the heating device of the cell culture workstation of the present invention. Figure 1 ; Figure 9 This is a schematic diagram of the heating device of the cell culture workstation of the present invention. Figure 2 : Figure 10 This is a flowchart of the control method for the cell culture workstation of the present invention; Figure 11 This is a flowchart of an embodiment of the control method for the cell culture workstation of the present invention.

[0024] List of reference numerals: 1. Chamber; 11. Refrigeration chamber; 12. Incubation chamber; 13. Testing chamber; 14. Material chamber; 15. Incubation chamber door; 16. Refrigeration chamber door; 121. Main shell; 122. Back panel; 123. Bottom plate; 151. Air inlet; 152. Air outlet; 20. First liquid storage component; 21. Liquid supply pipe; 22. Liquid supply pump; 23. Liquid supply control valve; 24. Second liquid storage component; 25. 1. Circulation pipe; 26. Circulation pump; 27. Circulation control valve; 28. Heater; 281. Annular heating element; 282. Horizontal heating element; 283. Annular fixing plate; 3. Incubator; 31. Liquid inlet; 32. Liquid outlet; 41. Detection mechanism; 42. Liquid extraction pipe; 43. Liquid extraction pump; 44. Waste liquid collection component; 45. Waste liquid pipe; 5. Clamping assembly; 51. First clamp; 52. Second clamp; 53. Connecting shaft; 54. Clamping component; 521. Second connecting structure; 6. Hanging assembly; 61. Strip-shaped body; 62. First hanging component; 63. Second hanging component; 611. Strip-shaped opening; 621. First hanging part; 622. First hanging post; 623. First limiting groove; 624. First conical part; 631. Second hanging part; 632. Second hanging post; 633. Second limiting groove; 634. Second conical part; 70. Storage component; 71. Conveying pipe; 72. Conveying pump; 73. Conveying control valve; 74. Third liquid storage component; 75. Flushing pipe; 76. Flushing control valve; 77. Fourth liquid storage component; 78. Protective liquid pipe; 79. Protective liquid control valve; 8. Heating device; 81. Air duct; 82. Heating component; 83. Fan; 811. Air inlet; 812. Air outlet. Detailed Implementation

[0025] Preferred embodiments of the present invention will now be described with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are merely illustrative of the technical principles of the present invention and are not intended to limit the scope of protection of the present invention.

[0026] It should be noted that in the description of this invention, terms such as "left" and "right," which indicate direction or positional relationship, are based on the direction or positional relationship shown in the accompanying drawings. This is merely for ease of description and does not indicate or imply that the device or element must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0027] Furthermore, it should be noted that, in the description of this invention, unless otherwise explicitly specified and limited, the terms "set," "connect," and "install" should be interpreted broadly, for example, referring to a fixed connection, a detachable connection, or an integral connection. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0028] Specifically, the present invention provides a cell culture workstation, such as Figures 1 to 4 As shown, the cell culture workstation of the present invention includes a housing 1, a first liquid storage component 20, a culture vessel 3, and a liquid supply component.

[0029] The housing 1 has a cold storage chamber 11 and a culture chamber 12. The first liquid storage component 20 is located in the cold storage chamber 11 and is used to store culture medium. The culture vessel 3 is located in the culture chamber 12 and can store and culture cells. The first liquid storage component 20 and the culture vessel 3 are connected by a liquid supply component, which is used to transport the culture medium in the first liquid storage component 20 to the culture vessel 3.

[0030] The refrigerator compartment 11 has a refrigerator door 16, which can close the refrigerator compartment 11. For example, the top of the refrigerator door 16 is pivotally connected to the box body 1, and the refrigerator door 16 can be rotated upward to open. The culture chamber 12 has a culture chamber door 15, which can close the culture chamber 12. For example, the left end of the culture chamber door 15 is pivotally connected to the box body 1, and the culture chamber door 15 can be rotated to the left to open.

[0031] For example, the cold storage chamber 11 is located near the top of the cabinet 1. The temperature of the cold storage chamber 11 is generally maintained at 2°C to 8°C for the cold storage of the culture medium. The culture medium is stored in the first liquid storage component 20, which is preferably a liquid storage bag. In order to maintain the temperature of the cold storage chamber 11 at the set temperature, a refrigeration device (not shown in the figure) is also provided in the cabinet 1 to regulate the temperature of the cold storage chamber 11. The culture chamber 12 is located below the cold storage chamber 11 and is used to provide the environment required for cell growth. The cells to be cultured are specifically located in the culture vessel 3, which is placed in the culture chamber 12. During the cell culture process, the culture medium is delivered to the culture vessel 3 through the liquid supply component for cell growth.

[0032] It should be noted that the present invention does not limit the specific type of refrigeration device. For example, a refrigeration device similar to that in an air conditioner can be used, or a semiconductor refrigeration device can be used.

[0033] Furthermore, it should be noted that the first liquid storage component 20 is not limited to the aforementioned liquid storage bag. For example, it can also be configured as a liquid storage bottle or a liquid storage container. Such adjustments and changes to the specific type of the first liquid storage component 20 do not deviate from the principles and scope of the present invention and should all be limited within the protection scope of the present invention. Of course, the present invention preferably configures the first liquid storage component 20 as a liquid storage bag.

[0034] Furthermore, it should be noted that the present invention does not limit the specific structural form of the culture vessel 3. Those skilled in the art can use any form of culture vessel in the prior art in practical applications, as long as it can achieve cell culture.

[0035] Preferably, such as Figure 1 and Figure 4 As shown, the culture device 3 of the present invention includes a shell and a plurality of hollow fiber tubes (not shown in the figure) installed inside the shell. The interior of the hollow fiber tubes forms channels for the flow of culture medium, and the cells are attached to the outer wall of the hollow fiber tubes.

[0036] The culture vessel 3 used in this invention is a hollow fiber culture vessel, which is a commonly used culture vessel in the prior art. Exemplarily, the outer shell of the culture vessel 3 is cylindrical, and the left end face and the right end face of the outer shell are respectively provided with an inlet 31 and an outlet 32. After the culture medium enters through the inlet 31, it flows into the hollow fiber tube and then slowly flows along the hollow fiber tube toward the outlet 32. The nutrients in the culture medium can pass through the tube wall of the hollow fiber tube and permeate to the outside of the hollow fiber tube for cell absorption.

[0037] Preferably, such as Figure 1 , Figure 2 and Figure 3 As shown, the liquid supply assembly of the present invention includes a first liquid supply assembly and a second liquid supply assembly. The second liquid supply assembly includes a second liquid storage component 24, a circulation pipe 25 and a circulation pump 26. The first liquid supply assembly is connected to the first liquid storage component 20 and the second liquid storage component 24. The circulation pump 26 is connected to the second liquid storage component 24 and the culture vessel 3 through the circulation pipe 25. The circulation pump 26 is used to circulate the culture medium between the second liquid storage component 24 and the culture vessel 3.

[0038] During cell culture, the culture medium in the first liquid storage component 20 is first transported to the second liquid storage component 24 through the first liquid supply component, and then the culture medium is circulated between the second liquid storage component 24 and the culture vessel 3 by the circulation pump 26.

[0039] For example, the inlet of the circulation pump 26 is connected to the second liquid storage component 24 through a circulation pipe 25, the outlet of the circulation pump 26 is connected to the inlet 31 of the culture vessel 3 through another circulation pipe 25, and the outlet 32 ​​of the culture vessel 3 is connected to the second liquid storage component 24 through another circulation pipe 25, forming a culture medium circulation loop. Preferably, the circulation pump 26 is a peristaltic pump.

[0040] Preferably, such as Figure 1 , Figure 2 and Figure 3 As shown, the first liquid supply assembly of the present invention includes a liquid supply pipe 21, a liquid supply pump 22, and a liquid supply control valve 23. The liquid supply pump 22 is connected to the first liquid storage component 20 and the second liquid storage component 24 through the liquid supply pipe 21. The liquid supply pump 22 can pump the culture medium in the first liquid storage component 20 to the second liquid storage component 24. The liquid supply control valve 23 is used to control the opening and closing of the liquid supply pipe 21.

[0041] For example, the inlet of the liquid supply pump 22 is connected to the first liquid storage component 20 through a liquid supply pipe 21, and the outlet of the liquid supply pump 22 is connected to the second liquid storage component 24 through another liquid supply pipe 21. The liquid supply control valve 23 is disposed on the liquid supply pipe 21 between the inlet of the liquid supply pump 22 and the first liquid storage component 20. Preferably, the liquid supply pump 22 is a peristaltic pump, the liquid supply pipe 21 is preferably a flexible hose, and the liquid supply control valve 23 is preferably a clamp valve.

[0042] Preferably, such as Figure 1 , Figure 2 and Figure 3 As shown, the second liquid supply assembly of the present invention further includes a circulation control valve 27, which is used to control the opening and closing of the circulation pipe 25.

[0043] For example, the circulation pipe 25 has three sections, located between the inlet end of the circulation pump 26 and the second liquid storage component 24, between the outlet end of the circulation pump 26 and the inlet 31 of the culture vessel 3, and between the outlet 32 ​​of the culture vessel 3 and the second liquid storage component 24, respectively. The circulation control valve 27 can be installed on any one of the three sections of the circulation pipe 25. Preferably, the circulation pipe 25 is a flexible hose, and the circulation control valve 27 is preferably a clamp valve.

[0044] Preferably, such as Figure 1 , Figure 2 and Figure 3 As shown, the liquid supply pump 22 and liquid supply control valve 23 of the first liquid supply component and the circulation pump 26 and circulation control valve 27 of the second liquid supply component are all installed on the back plate 122 of the culture chamber 12. The back plate 122 is detachably and fixedly connected to the main shell 121 of the culture chamber 12.

[0045] By mounting the liquid supply pump 22, liquid supply control valve 23, circulation pump 26, and circulation control valve 27 all on the back plate 122 of the culture chamber 12, it is easy to replace them as a whole according to different culture programs. Specifically, there are many different culture programs for cell culture, and different culture programs directly affect the number and layout of control valves and pumps. By integrating all the control valves and pumps required for cell culture onto the back plate 122 of the culture chamber 12, when a different culture program needs to be changed, the back plate 122 can be directly removed for complete replacement.

[0046] Preferably, such as Figure 1 As shown, a plurality of first liquid storage components 20 are provided in the cold storage compartment 11. Each first liquid storage component 20 is connected to a liquid supply pipe 21 (referred to as a branch liquid supply pipe), and each branch liquid supply pipe is provided with a corresponding liquid supply control valve 23. The plurality of branch liquid supply pipes are all connected to the same liquid supply pipe 21 (referred to as the main liquid supply pipe), and the main liquid supply pipe is connected to the liquid inlet end of the liquid supply pump 22.

[0047] In other words, the first liquid supply assembly includes multiple liquid supply control valves 23 and multiple liquid supply pipes 21. The multiple liquid supply pipes 21 are respectively connected to the corresponding first liquid storage component 20. The multiple liquid supply control valves 23 are respectively used to control the opening and closing of the corresponding liquid supply pipes 21. The multiple liquid supply control valves 23 are all installed on the back plate 122 of the culture chamber 12.

[0048] For example, five first liquid storage components 20 are provided in the cold storage compartment 11, and there are also five liquid supply control valves 23, each corresponding to one first liquid storage component 20. The five liquid supply control valves 23 are normally in the off state. When the culture medium in a certain first liquid storage component 20 needs to be used, the corresponding liquid supply control valve 23 is turned on to start the liquid supply.

[0049] Preferably, such as Figure 3 As shown, the second liquid supply assembly of the present invention further includes a heater 28, which is used to heat the culture medium in the second liquid storage component 24.

[0050] Since the first liquid storage component 20 is located in the cold storage chamber 11, the temperature of the culture medium is relatively low. The culture medium in the first liquid storage component 20 is first transported to the second liquid storage component 24 through the first liquid supply component. After the culture medium in the second liquid storage component 24 is heated by the heater 28, the culture medium is then allowed to enter the incubator 3.

[0051] It should be noted that the present invention does not limit the specific structural form of the heater 28. For example, it can be set as a heating wire, heating plate or heating tube, etc. Such adjustments and changes to the specific structural form of the heater 28 do not deviate from the principle and scope of the present invention and should be limited to the protection scope of the present invention.

[0052] Preferably, such as Figure 3 As shown, the heater 28 of the present invention includes an annular heating element 281, which is disposed around the second liquid storage member 24.

[0053] By using an annular heating element 281 to heat the culture medium in the second liquid storage component 24, the temperature of the culture medium can be made more uniform. For example, the second liquid storage component 24 is a liquid storage bottle, and the annular heating element 281 forms a cylindrical structure in which the liquid storage bottle is inserted.

[0054] Preferably, such as Figure 3 As shown, the heater 28 of the present invention also includes a horizontal heating element 282, the bottom end of the annular heating element 281 is fixedly connected to or integrally formed with the horizontal heating element 282, the annular heating element 281 and the horizontal heating element 282 together form a heating cavity, and the second liquid storage component 24 is located in the heating cavity.

[0055] The annular heating element 281 is vertically arranged to form the side wall of the heating chamber, and the horizontal heating element 282 is horizontally arranged to form the bottom wall of the heating chamber. The horizontal heating element 282 supports the bottom of the second liquid storage component 24.

[0056] Preferably, such as Figure 3 As shown, the heater 28 of the present invention also includes an annular fixing plate 283. The top end of the annular heating element 281 is fixedly connected to or integrally formed with the annular fixing plate 283. The bottom plate 123 of the culture chamber 12 is provided with an installation port. The size of the installation port is larger than the outer diameter of the annular heating element 281 and smaller than the outer diameter of the annular fixing plate 283. The annular heating element 281 and the horizontal heating element 282 pass through the installation port, and the annular fixing plate 283 abuts against the upper surface of the bottom plate 123. That is, the annular heating element 281 and the horizontal heating element 282 are located below the bottom plate 123, and the annular fixing plate 283 is located above the bottom plate 123, which is equivalent to hanging the heater 28 on the bottom plate 123.

[0057] Preferably, such as Figure 1 and Figure 2 As shown, a hanging assembly 6 is provided in the refrigeration compartment 11 of the box 1, and the first liquid storage component 20 (liquid storage bag) is hung on the hanging assembly 6.

[0058] Preferably, such as Figure 1 and Figure 2 As shown, there are multiple hanging components 6, which are spaced apart along the length of the refrigerator compartment 11.

[0059] For example, multiple hanging components 6 in the refrigerator compartment 11 are distributed at intervals in the left-right direction, and multiple liquid storage bags are provided in the refrigerator compartment 11, with each liquid storage bag hanging on a hanging component 6.

[0060] Preferably, such as Figures 5 to 7 As shown, the mounting component 6 of the present invention is configured to be suitable for liquid storage bags of different sizes.

[0061] The liquid storage bag is provided with a first hanging hole and a second hanging hole that cooperate with the hanging component 6. The spacing between the first hanging hole and the second hanging hole is different for different sizes of liquid storage bags. The hanging component 6 of the present invention can adapt to a variety of different sizes of liquid storage bags, making it more convenient to use.

[0062] Preferably, such as Figures 5 to 7 As shown, the mounting component 6 of the present invention includes a horizontally arranged strip-shaped body 61 and a first mounting member 62 and a second mounting member 63 mounted on the strip-shaped body 61. The first mounting member 62 and the second mounting member 63 are distributed along the length direction of the strip-shaped body 61, and at least one of the first mounting member 62 and the second mounting member 63 is capable of moving along the length direction of the strip-shaped body 61.

[0063] The first hanging component 62 and the second hanging component 63 respectively cooperate with the first hanging hole and the second hanging hole on the liquid storage bag. By adjusting the distance between the first hanging component 62 and the second hanging component 63, liquid storage bags of different sizes can be adapted.

[0064] It should be noted that, in practical applications, those skilled in the art can configure the first hanging member 62 and the second hanging member 63 to both be able to move along the length direction of the strip-shaped body 61, or they can configure only the first hanging member 62 or the second hanging member 63 to be able to move along the length direction of the strip-shaped body 61.

[0065] Preferably, such as Figures 5 to 7 As shown, the strip-shaped body 61 of the hanging component 6 of the present invention has a shell structure. The bottom of the strip-shaped body 61 is provided with a strip-shaped opening 611 extending along the length direction of the strip-shaped body 61. The top of the first hanging member 62 is provided with a first hooking part 621. The first hooking part 621 extends through the strip-shaped opening 611 into the inside of the strip-shaped body 61 and is locked inside the strip-shaped body 61. The first hanging member 62 can move along the length direction of the strip-shaped opening 611.

[0066] For example, the strip-shaped body 61 is a rectangular shell. The top surface of the strip-shaped body 61 is fixedly connected to the inner top wall of the refrigerator compartment 11. The strip-shaped body 61 extends in the front-back direction. The bottom surface of the strip-shaped body 61 has a strip-shaped opening 611 that communicates with the interior of the strip-shaped body 61. The top of the first hanging member 62 is provided with a first hanging part 621. The first hanging part 621 is horizontally arranged and located inside the strip-shaped body 61. The first hanging member 62 is suspended below the strip-shaped body 61 through the first hanging part 621. The first hanging member 62 can move back and forth along the strip-shaped opening 611 to adjust the distance between the first hanging member 62 and the second hanging member 63 to adapt to different sizes of liquid storage bags.

[0067] Preferably, such as Figure 5 and Figure 6 As shown, the second hanging member 63 of the hanging assembly 6 of the present invention is provided with a second hanging part 631 at its top end. The second hanging part 631 extends through the strip opening 611 into the inside of the strip body 61 and is locked inside the strip body 61. The second hanging member 63 can move along the length direction of the strip opening 611.

[0068] Similar to the first hanging member 62, the second hanging member 63 is also suspended below the strip-shaped body 61 via the second hook portion 631 at its top. The second hanging member 63 can also move back and forth along the strip-shaped opening 611 to adjust the distance between the second hanging member 63 and the first hanging member 62 to accommodate liquid storage bags of different sizes. It should be noted that, in practical applications, those skilled in the art can also set the first hanging member 62 to be stationary and only set the second hanging member 63 to be movable.

[0069] Preferably, such as Figures 5 to 7 As shown, the first hanging component 62 is provided with a horizontally arranged first hanging post 622, and the second hanging component 63 is provided with a horizontally arranged second hanging post 632. The first hanging post 622 and the second hanging post 632 pass through the first hanging hole and the second hanging hole at the top of the liquid storage bag, respectively. It should be noted that, in practical applications, those skilled in the art can also make the first hanging post 622 and the second hanging post 632 both inclined upwards.

[0070] Preferably, such as Figures 5 to 7 As shown, a first hanging post 622 is provided on the left and right sides of the first hanging component 62, and a second hanging post 632 is provided on the left and right sides of the second hanging component 63.

[0071] By setting a first hanging post 622 on each of the left and right sides of the first hanging component 62 and a second hanging post 632 on each of the left and right sides of the second hanging component 63, two liquid storage bags can be suspended simultaneously on the same hanging component 6.

[0072] Preferably, such as Figures 5 to 7 As shown, a first limiting groove 623 is provided on the first hook post 622, and the first hanging hole on the liquid storage bag is located in the first limiting groove 623. By providing the first limiting groove 623 on the first hook post 622, after the liquid storage bag is installed on the first hook post 622, the first hanging hole is located at the first limiting groove 623, which can prevent the liquid storage bag from separating from the first hook post 622, thereby preventing the liquid storage bag from falling off.

[0073] Preferably, such as Figures 5 to 7 As shown, the insertion end of the first hook post 622 is provided with a first tapered portion 624. By providing the first tapered portion 624 at the insertion end of the first hook post 622, the first hook post 622 can more easily pass through the first hook hole on the liquid storage bag when installing the liquid storage bag.

[0074] For example, such as Figure 7 As shown, the right end of the first hanging post 622 located on the right side of the first hanging member 62 is its insertion end, and the right end of the first hanging post 622 is provided with a first tapered part 624.

[0075] Preferably, such as Figure 5 and Figure 6 As shown, a second limiting groove 633 is provided on the second hanging post 632, and the second hanging hole on the liquid storage bag is located in the second limiting groove 633.

[0076] By setting a second limiting groove 633 on the second hanging post 632, after the liquid storage bag is installed on the second hanging post 632, the second hanging hole is located at the second limiting groove 633, which can prevent the liquid storage bag from separating from the second hanging post 632, thereby preventing the liquid storage bag from falling off.

[0077] Preferably, such as Figure 5 and Figure 6 As shown, the insertion end of the second hook post 632 is provided with a second conical portion 634. By providing the second conical portion 634 at the insertion end of the second hook post 632, the second hook post 632 can more easily pass through the second hook hole on the liquid storage bag when installing the liquid storage bag.

[0078] It should be noted that regarding the formation of the first limiting groove 623 and the second limiting groove 633 mentioned above, a portion of the middle part of the hanging post can be removed to form a groove, thus reducing the diameter of the middle part of the hanging post to form a limiting groove. Alternatively, the hanging post can be configured to consist of three parts, with the diameter of the middle part being smaller than the diameters of the two side parts, so as to form a limiting groove in the middle part of the hanging post, etc. Such flexible adjustments and changes do not deviate from the principles and scope of the present invention and should all be limited to the protection scope of the present invention.

[0079] Preferably, such as Figure 1 and Figure 2 As shown, the housing 1 of the present invention also includes a detection chamber 13, a liquid extraction assembly, and a detection mechanism 41 installed in the detection chamber 13. The liquid extraction assembly is connected to the liquid supply assembly and the detection mechanism 41. The liquid extraction assembly can extract the culture medium from the liquid supply assembly and deliver it to the detection mechanism 41. The detection mechanism 41 is used to detect the indicators of the culture medium.

[0080] For example, the indicator of the culture medium is glucose concentration. As the cells grow, the glucose concentration in the culture medium will gradually decrease. The glucose concentration in the culture medium is detected by the detection device 41. When the glucose concentration is lower than the lower limit, the culture medium is replaced in time.

[0081] It should be noted that the indicators of the culture medium are not limited to the glucose concentration mentioned above. In practical applications, those skilled in the art can determine the specific content of the culture medium indicators according to the specific cell type.

[0082] Preferably, such as Figure 1 and Figure 2 As shown, one end of the liquid extraction component is connected to the second liquid storage component 24 or the circulation pipe 25 of the second liquid supply component, and the other end of the liquid extraction component is connected to the detection mechanism 41.

[0083] Preferably, such as Figure 1 and Figure 2 As shown, the liquid extraction assembly of the present invention includes a liquid extraction pipe 42, a liquid extraction pump 43, and a liquid extraction control valve (not shown in the figure). The liquid inlet of the liquid extraction pump 43 is connected to the second liquid storage component 24 or the circulation pipe 25 through the liquid extraction pipe 42, and the liquid outlet of the liquid extraction pump 43 is connected to the detection mechanism 41 through the liquid extraction pipe 42. The liquid extraction control valve is used to control the opening and closing of the liquid extraction pipe 42.

[0084] For example, the inlet of the pump 43 is connected to the circulation pipe 25 between the second liquid storage component 24 and the outlet 32 ​​of the culture vessel 3 via a section of the pumping pipe 42, and the outlet of the pump 43 is connected to the detection mechanism 41 via another section of the pumping pipe 42. A pumping control valve is located on the pumping pipe 42 between the outlet of the pump 43 and the detection mechanism 41. When it is necessary to extract culture medium from the second liquid supply component for testing, the pumping control valve is opened, the pump 43 is started, and the culture medium is delivered to the detection mechanism 41 in the detection chamber 13 for testing. Preferably, the pump 43 is a peristaltic pump, the pumping pipe 42 is a flexible tube, and the pumping control valve is a clamp valve.

[0085] Preferably, such as Figure 1 , Figure 2 and Figure 3As shown, the liquid extraction pump 43 and the liquid extraction control valve of the liquid extraction assembly of the present invention are both mounted on the back plate 122 of the culture chamber 12. By mounting the liquid extraction pump 43 and the liquid extraction control valve on the back plate 122 of the culture chamber 12, it is convenient to replace the entire assembly according to different culture programs.

[0086] Preferably, such as Figure 1 , Figure 2 and Figure 3 As shown, the cell culture workstation of the present invention also includes a waste liquid collection component 44, a waste liquid pipe 45, and a waste liquid control valve (not shown in the figure). The waste liquid collection component 44 is connected to the liquid pump 43 through the waste liquid pipe 45. The liquid pump 43 can pump the waste liquid in the second liquid storage component 24 to the waste liquid collection component 44. The waste liquid control valve is used to control the opening and closing of the waste liquid pipe 45. The waste liquid control valve is installed on the back plate 122.

[0087] When it is necessary to replace the culture medium, the original culture medium in the second storage component 24 should be transferred to the waste liquid collection component 44 first. Then, the waste liquid control valve should be opened, the pump 43 should be started, and the culture medium in the second storage component 24 should be transferred to the waste liquid collection component 44. Then, the new culture medium should be transferred to the second storage component 24 through the first supply component.

[0088] The waste liquid collection component 44 is preferably configured as a waste liquid collection bag, the waste liquid pipe 45 is preferably configured as a flexible hose, and the waste liquid control valve is preferably configured as a clamp valve.

[0089] Preferably, such as Figure 1 , Figure 8 and Figure 9 As shown, the cell culture workstation of the present invention also includes a heating device 8, which can adjust the temperature in the culture chamber 12 so that the temperature in the culture chamber 12 meets the set cell culture temperature.

[0090] The cell culture temperature is generally around 37°C. The temperature in the culture chamber 12 is adjusted by the heating device 8 to maintain the temperature at around 37°C.

[0091] It should be noted that the present invention does not limit the specific installation position of the heating device 8. For example, the heating device 8 can be installed on the main shell 121 of the culture chamber 12, or it can be installed on the door 15 of the culture chamber, etc.

[0092] Preferably, such as Figure 1 , Figure 8 and Figure 9As shown, the heating device 8 of the present invention is installed in the culture chamber door 15 of the culture chamber 12. An air inlet 151 and an air outlet 152 are provided on the inner side wall of the culture chamber door 15. The heating device 8 can draw in the air in the culture chamber 12 through the air inlet 151, heat it, and then discharge it through the air outlet 152.

[0093] For example, the air inlet 151 is located above the air outlet 152. The air in the culture chamber 12 enters the heating device 8 through the air inlet 151, is heated by the heating device 8, and then returns to the culture chamber 12 through the air outlet 152 for circulation, which helps to keep the temperature in the culture chamber 12 stable.

[0094] Preferably, such as Figure 1 , Figure 8 and Figure 9 As shown, the heating device 8 of the present invention includes an air duct 81, a heating component 82 and a fan 83 installed in the air duct 81. The air inlet end 811 of the air duct 81 is connected to or located near the air inlet 151 on the door of the culture chamber 15. The air outlet end 812 of the air duct 81 is connected to or located near the air outlet 152 on the door of the culture chamber 15.

[0095] When the fan 83 starts running, the air in the culture chamber 12 is drawn into the air inlet 151 of the culture chamber door 15, and then enters the air duct 81. It flows along the air duct 81, and its temperature rises when it flows through the heating component 82. It is then discharged from the air outlet 812 of the air duct 81 and returns to the culture chamber 12 through the air outlet 152 on the culture chamber door 15.

[0096] It should be noted that the present invention does not limit the specific structural form of the heating component 82, for example, it can be set as a heating plate, heating wire or heating tube, etc.

[0097] Preferably, such as Figure 8 and Figure 9 As shown, the heating component 82 of the present invention is configured as a heating plate.

[0098] Preferably, such as Figure 8 and Figure 9 As shown, a gap is formed between the heating element 82 and the inner wall of the air duct 81. By forming a gap between the heating element 82 and the inner wall of the air duct 81, the heating efficiency of the heating element 82 can be improved.

[0099] For example, the heating member 82 is a vertically arranged heating plate, with a gap between the left side of the heating plate and the left side wall of the air duct 81, and a gap between the right side of the heating plate and the right side wall of the air duct 81.

[0100] Preferably, such as Figure 1 , Figure 3 and Figure 4 As shown, the cell culture workstation of the present invention also includes a drive device, which is connected to the culture vessel 3 and can drive the culture vessel 3 to rotate.

[0101] During cell culture, the culture vessel 3 can be rotated by a drive device to make the culture medium in the culture vessel 3 more uniform, which is conducive to cell growth.

[0102] It should be noted that in practical applications, it is not necessary to drive the incubator 3 to rotate a full circle. Instead, the drive device should drive the incubator 3 to rotate back and forth, for example, first rotating it forward at a certain angle and then rotating it in the opposite direction.

[0103] Preferably, such as Figure 4 As shown, the rotation axis of the culture vessel 3 extends horizontally and is perpendicular to the axis of the culture vessel 3. Exemplarily, the rotation axis of the culture vessel 3 extends in the front-to-back direction.

[0104] Preferably, such as Figure 3 and Figure 4 As shown, the driving device of the present invention includes a drive motor (not shown in the figure) and a clamping assembly 5. The drive motor is connected to the clamping assembly 5 and can drive the clamping assembly 5 to rotate. The clamping assembly 5 is used to clamp the incubator 3.

[0105] First, the culture vessel 3 is clamped and fixed by the clamping assembly 5, and then the culture vessel 3 is rotated by the drive motor. For example, the drive motor is installed on the outer wall of the back plate 122 of the culture chamber 12, and the drive shaft of the drive motor passes through the back plate 122 and extends into the culture chamber 12 to be fixedly connected to the clamping assembly 5.

[0106] Preferably, such as Figure 4 As shown, the clamping assembly 5 includes a first clamp 51, a second clamp 52, and a connecting shaft 53. The first clamp 51 is fixedly connected to one end of the connecting shaft 53 or integrally formed therewith. The other end of the connecting shaft 53 is fixedly connected to the drive shaft of the drive motor. One end of the first clamp 51 is pivotally connected to one end of the second clamp 52. The other end of the first clamp 51 is provided with a first connecting structure, and the other end of the second clamp 52 is provided with a second connecting structure 521. The first connecting structure and the second connecting structure 521 are detachably fixedly connected. The culturer 3 is clamped between the first clamp 51 and the second clamp 52.

[0107] For example, the connecting shaft 53 is horizontally arranged and perpendicular to the axis of the culture vessel 3. The first gripper 51 is located below the second gripper 52. The first connecting structure is a first connecting hole provided on the first gripper 51, and the second connecting structure 521 is a second connecting hole provided on the second gripper 52. When installing the culture vessel 3, the second gripper 52 is first rotated upward, and then the culture vessel 3 is placed on the first gripper 51. Then the second gripper 52 is rotated downward to close with the first gripper 51. The first gripper 51 and the second gripper 52 form a cylindrical cavity. The first gripper 51 and the second gripper 52 are both in contact with the outer shell of the culture vessel 3. Finally, the first connecting hole and the second connecting hole are connected and fixed by a bolt.

[0108] Preferably, such as Figure 4 As shown, the clamping assembly 5 of the present invention further includes a pressing member 54. The first clamp 51 or the second clamp 52 is provided with a threaded hole, and the pressing member 54 is provided with a thread that matches the threaded hole. The pressing member 54 is installed in the threaded hole, and one end of the pressing member 54 can pass through the threaded hole and abut against the outer wall of the incubator 3.

[0109] For example, a threaded hole is provided on the second gripper 52, and the clamping member 54 is a clamping bolt. The clamping bolt is screwed into the threaded hole. After the culturer 3 is installed between the first gripper 51 and the second gripper 52, the clamping bolt is rotated downward so that the bottom end of the clamping bolt abuts against the outer shell of the culturer 3, which can prevent the culturer 3 from coming out between the first gripper 51 and the second gripper 52.

[0110] Preferably, such as Figure 1 and Figure 2 As shown, the cell culture workstation of the present invention further includes a storage component 70 and a transport component. The storage component 70 stores cells and is located in a cold storage chamber 11. The transport component is connected to the storage component 70 and the culture vessel 3 and is used to transport the cells in the storage component 70 to the culture vessel 3.

[0111] The cells stored in the storage component 70 are cell seeds, which can be transported to the outer shell of the culture vessel 3 by the transport component.

[0112] Preferably, such as Figure 1 , Figure 2 and Figure 3 As shown, the delivery assembly includes a delivery pipe 71, a delivery pump 72, and a delivery control valve 73. The delivery pump 72 is connected to the storage component 70 and the culture vessel 3 through the delivery pipe 71. The delivery pump 72 is used to deliver cells in the storage component 70 to the culture vessel 3. The delivery control valve 73 is used to control the opening and closing of the delivery pipe 71. Both the delivery pump 72 and the delivery control valve 73 are installed on the back plate 122 of the culture chamber 12.

[0113] For example, the storage component 70 is a storage bag containing cell fluid. A hanging assembly 6 for hanging the storage bag is also provided in the refrigerator compartment 11. The storage bag is connected to the inlet of the delivery pump 72 via a delivery pipe 71. A delivery control valve 73 controls the opening and closing of this delivery pipe 71. The outlet of the delivery pump 72 is connected to the culture vessel 3 via another delivery pipe 71. Preferably, the delivery pump 72 is a peristaltic pump, the delivery pipe 71 is a flexible tube, and the delivery control valve 73 is a clamp valve.

[0114] Preferably, such as Figure 1 , Figure 2 and Figure 3 As shown, the cell culture workstation of the present invention also includes a third liquid storage component 74, a rinsing tube 75, and a rinsing control valve 76. The third liquid storage component 74 is located in the cold storage chamber 11 and stores rinsing solution. The third liquid storage component 74 is connected to a transfer pump 72 through the rinsing tube 75. The transfer pump 72 can deliver the rinsing solution in the third liquid storage component 74 to the culture vessel 3. The rinsing control valve 76 is used to control the opening and closing of the rinsing tube 75 and is installed on the back plate 122 of the culture chamber 12. By installing the rinsing control valve 76 on the back plate 122 of the culture chamber 12, it is convenient to replace the entire unit according to different culture programs.

[0115] For example, the third liquid storage component 74 is also a storage bag containing rinsing solution (e.g., physiological saline). A hanging assembly 6 for mounting the third liquid storage component 74 is also provided in the refrigerator compartment 11. The third liquid storage component 74 is connected to the inlet of the delivery pump 72 via a rinsing pipe 75. A rinsing control valve 76 controls the opening and closing of the rinsing pipe 75. Before cell culture, the culture vessel 3 needs to be rinsed with rinsing solution. Opening the rinsing control valve 76 starts the delivery pump 72, delivering the rinsing solution from the third liquid storage component 74 to the culture vessel 3 for rinsing. Preferably, the rinsing pipe 75 is a flexible tube, and the rinsing control valve 76 is preferably a clamp valve.

[0116] Preferably, such as Figure 1 , Figure 2 and Figure 3 As shown, the cell culture workstation of the present invention also includes a fourth liquid storage component 77, a protective liquid pipe 78, and a protective liquid control valve 79. The fourth liquid storage component 77 is located in the cold storage chamber 11 and stores protective liquid. The fourth liquid storage component 77 is connected to a transfer pump 72 through the protective liquid pipe 78. The transfer pump 72 can deliver the protective liquid in the fourth liquid storage component 77 to the culture vessel 3. The protective liquid control valve 79 is used to control the opening and closing of the protective liquid pipe 78 and is installed on the back plate 122 of the culture chamber 12. By installing the protective liquid control valve 79 on the back plate 122 of the culture chamber 12, it is convenient to replace the entire unit according to different culture programs.

[0117] For example, the fourth liquid storage component 77 is also a storage bag containing a protective liquid (e.g., Schereson protective liquid). A hanging assembly 6 for hanging the fourth liquid storage component 77 is also provided in the refrigerator compartment 11. The fourth liquid storage component 77 is connected to the inlet of the delivery pump 72 via a protective liquid pipe 78. A protective liquid control valve 79 is used to control the opening and closing of the protective liquid pipe 78. After cell culture is completed, protective liquid needs to be injected into the culture vessel 3. The protective liquid control valve 79 is opened, and the delivery pump 72 is started to deliver the protective liquid in the fourth liquid storage component 77 to the culture vessel 3. Preferably, the protective liquid pipe 78 is a flexible tube, and the protective liquid control valve 79 is preferably a clamp valve.

[0118] Preferably, such as Figure 1 and Figure 2 As shown, the housing 1 also includes a material chamber 14, which can store culture consumables. The culture chamber 12 and the detection chamber 13 are arranged side by side. The cold storage chamber 11 is located above the culture chamber 12 and the detection chamber 13, and the material chamber 14 is located below the culture chamber 12 and the detection chamber 13.

[0119] The various liquid storage components and the hoses used to connect the liquid storage components described above are all culture consumables. These culture consumables can be stored in the material chamber 14. Each time a cell culture is completed, new culture consumables need to be replaced.

[0120] Based on any of the cell culture workstations described above, this invention also provides a control method for the cell culture workstation, such as... Figure 10 As shown, the control method of the present invention includes the following steps: S100: detecting whether there are air bubbles in the circulation pipe 25 connected to the liquid outlet 32 ​​of the culture vessel; S200: selectively driving the culture vessel 3 to rotate according to the detection result, so as to expel the air in the culture vessel 3.

[0121] As is known from the background art, if air is trapped in the culture vessel, some cells will not be able to contact the culture medium, resulting in poor culture effect and culture failure. Therefore, this invention performs air bubble detection during cell culture, specifically detecting whether there are air bubbles in the circulation tube 25 connected to the outlet 32 ​​of the culture vessel 3. For example, a bubble sensor can be installed in the culture chamber to detect whether there are air bubbles in the circulation tube 25. If air bubbles are detected in the circulation tube 25, it indicates that there is air trapped in the culture vessel 3, and the culture vessel 3 needs to be rotated by a drive device to expel the air.

[0122] Preferably, such as Figure 11As shown, step S200, "selectively driving the incubator 3 to rotate according to the detection results", specifically includes: S210: when bubbles are detected and the cumulative time of bubbles appearing within the detection cycle is greater than a first preset time, the incubator 3 is driven to rotate.

[0123] For example, the detection cycle is 3000 seconds, the first preset time is 10 seconds. After the detection starts, during the detection cycle from 0 to 3000 seconds, bubbles appear in the circulation tube at the 30th second, and the cumulative time of the bubbles is increased by 1 second. Bubbles appear again at the 60th second, and the cumulative time of the bubbles is increased by another 1 second, for a total of 2 seconds. Bubbles appear consecutively at the 70th and 71st seconds, and the cumulative time of the bubbles is increased by another 2 seconds, for a total of 4 seconds. And so on. When the cumulative time of the bubbles is greater than 10 seconds, the drive device is controlled to drive the incubator 3 to rotate in order to expel the air from the incubator 3.

[0124] It should be noted that the specific duration of the detection cycle is not limited to the aforementioned 3000 seconds. In practical applications, those skilled in the art can flexibly set the specific duration of the detection cycle based on experiments or experience. For example, those skilled in the art can also set the duration of the detection cycle to 1500 seconds, 2000 seconds, 2500 seconds, 4000 seconds, 4500 seconds, 5000 seconds, 6000 seconds, or 7000 seconds, etc.

[0125] Furthermore, it should be noted that the specific value of the first preset time is not limited to the aforementioned 10 seconds. In practical applications, those skilled in the art can flexibly set the specific value of the first preset time based on experiments or experience. For example, those skilled in the art can also set the first preset time to 5 seconds, 6 seconds, 7 seconds, 8 seconds, 9 seconds, 11 seconds, 12 seconds, 15 seconds, or 20 seconds, etc.

[0126] Preferably, such as Figure 11 As shown, the control method of the present invention further includes step S300: during the detection cycle, when the cumulative time for the appearance of bubbles is greater than a second preset time, the rotation speed of the incubator 3 is reduced; wherein, the second preset time is greater than the first preset time.

[0127] After the incubator is driven to rotate by the drive device, the bubbles are continuously detected. When the cumulative time for the appearance of bubbles is greater than the second preset time, it indicates that there are too many bubbles in the incubator and the rotation speed of the incubator needs to be reduced. Reducing the rotation speed of the incubator is more conducive to the expulsion of air from the incubator.

[0128] For example, the detection cycle is 3000 seconds, the first preset time is 10 seconds, and the second preset time is 60 seconds. After the detection starts, within the detection cycle of 0 to 3000 seconds, when the cumulative time for the appearance of bubbles is greater than 10 seconds, the drive device is started to drive the incubator to rotate at the first speed to continue detecting bubbles. Within the detection cycle of 0 to 3000 seconds, when the cumulative time for the appearance of bubbles is greater than 60 seconds, the drive device is controlled to drive the incubator to rotate at the second speed, wherein the second speed is less than the first speed.

[0129] It should be noted that the specific value of the second preset time is not limited to the aforementioned 60 seconds. In practical applications, those skilled in the art can flexibly set the specific value of the second preset time based on experiments or experience. For example, those skilled in the art can also set the second preset time to 30 seconds, 40 seconds, 50 seconds, 70 seconds, 80 seconds, 90 seconds, 100 seconds, 120 seconds, or 150 seconds, etc.

[0130] Furthermore, it should be noted that this invention does not limit the specific values ​​of the first and second speeds of the incubator. Those skilled in the art can flexibly set the specific values ​​of the first and second speeds based on experiments or experience in practical applications. For example, the first speed can be set to 6 degrees / second to 10 degrees / second, and the second speed can be set to 3 degrees / second to 5 degrees / second.

[0131] Furthermore, it should be noted that when the rotation speed of the incubator 3 decreases to the second speed, it is preferable to continue detecting bubbles in the circulation tube. Taking a detection cycle of 3000 seconds as an example, the first detection cycle is from 0 to 3000 seconds, the second detection cycle is from 1 to 3001 seconds, the third detection cycle is from 2 to 3002 seconds, and so on. Assuming the first bubble appears at the 30th second, the cumulative time for bubble appearance within the detection cycle from 30 to 3030 seconds is 65 seconds. When the detection time reaches the detection cycle from 31 to 3031 seconds, the cumulative time for bubble appearance needs to be reduced by 1 second, which is the time for the bubble to appear at the 30th second. The cumulative time for bubble appearance then becomes 64 seconds. When the cumulative time for bubble appearance decreases to less than the second preset time, the drive device is controlled to drive the incubator to increase its speed and rotate at the first speed. When the cumulative time for bubble appearance decreases to less than the first preset time, the rotation of the incubator is stopped.

[0132] Preferably, such as Figure 11 As shown, the control method of the present invention further includes step S400: if the cumulative time for the appearance of bubbles is greater than a third preset time within the detection cycle, the cell culture workstation will issue an alarm prompt; wherein the third preset time is greater than the second preset time.

[0133] When the rotation speed of the culture vessel is reduced to the second speed, the air bubbles in the circulation tube continue to be detected. When the cumulative time for air bubbles to appear in the circulation tube is greater than the third preset time, it indicates that the pipeline is very likely to be faulty, and the cell culture workstation needs to issue an alarm to remind the staff to carry out maintenance.

[0134] It should be noted that the present invention does not limit the specific value of the third preset time. In practical applications, those skilled in the art can flexibly set the specific value of the third preset time based on experiments or experience. For example, those skilled in the art can set the third preset time to 300 seconds, 400 seconds, 500 seconds, 600 seconds, 700 seconds, 800 seconds, 900 seconds, or 1000 seconds, etc.

[0135] Preferably, the step of “driving the culture vessel 3 to rotate” specifically includes: driving the culture vessel 3 to swing back and forth.

[0136] For example, such as Figure 1 and Figure 4 As shown, the culture vessel 3 is horizontally positioned, with its rotation axis extending horizontally and perpendicular to its length. The inlet 31 is located at the left end of the length direction of the culture vessel 3, and the outlet 32 ​​is located at the right end of the length direction of the culture vessel 3. When driving the culture vessel to rotate, it is first driven to rotate clockwise. After rotating 30 degrees, the culture vessel is driven to rotate counterclockwise. After rotating 30 degrees, the culture vessel returns to the horizontal position. It then continues to rotate counterclockwise by 30 degrees, and then the culture vessel is driven to rotate clockwise again, thus causing the culture vessel to swing back and forth.

[0137] By making the incubator swing back and forth, it is easier to expel the air from the incubator.

[0138] Those skilled in the art will understand that although some embodiments herein include certain features included in other embodiments but not others, combinations of features from different embodiments are intended to be within the scope of this application and form different embodiments. For example, any of the claimed embodiments in the claims of this application can be used in any combination.

[0139] The technical solution of the present invention has been described above with reference to the preferred embodiments shown in the accompanying drawings. However, it will be readily understood by those skilled in the art that the scope of protection of the present invention is obviously not limited to these specific embodiments. Without departing from the principles of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after such changes or substitutions will all fall within the scope of protection of the present invention.

Claims

1. A control method for a cell culture workstation, characterized in that, The cell culture workstation includes a culture vessel (3), a circulation tube (25), and a drive device. The culture vessel (3) can store and culture cells. The culture vessel (3) has an inlet (31) and an outlet (32), both of which are connected to the circulation tube (25). The circulation tube (25) is used to guide the circulation of the culture medium. The drive device is connected to the culture vessel (3) and can drive the culture vessel (3) to rotate. The control method includes: Detect whether there are air bubbles in the circulation pipe (25) connected to the liquid outlet (32); Based on the test results, the incubator (3) is selectively driven to rotate in order to expel the air from the incubator (3).

2. The control method for the cell culture workstation according to claim 1, characterized in that, The step of "selectively driving the incubator (3) to rotate according to the test results" specifically includes: When bubbles are detected and the cumulative time of bubbles appearing within the detection period is greater than a first preset time, the incubator (3) is driven to rotate.

3. The control method for the cell culture workstation according to claim 2, characterized in that, The control method further includes: If the cumulative time for the appearance of bubbles during the detection period is greater than the second preset time, the rotation speed of the incubator (3) shall be reduced. The second preset time is longer than the first preset time.

4. The control method for the cell culture workstation according to claim 3, characterized in that, The control method further includes: If the cumulative time for the appearance of bubbles exceeds a third preset time within the detection period, the cell culture workstation will issue an alarm. The third preset time is greater than the second preset time.

5. The control method for the cell culture workstation according to claim 1, characterized in that, The step of "driving the incubator (3) to rotate" specifically includes: driving the incubator (3) to swing back and forth.

6. The control method for the cell culture workstation according to claim 1, characterized in that, The driving device includes a drive motor and a clamping assembly (5). The drive motor is connected to the clamping assembly (5) and can drive the clamping assembly (5) to rotate. The clamping assembly (5) is used to clamp the incubator (3); and / or The rotation axis of the culture vessel (3) extends horizontally and is perpendicular to the axis of the culture vessel (3).

7. The control method for the cell culture workstation according to claim 6, characterized in that, The clamping assembly (5) includes a first gripper (51), a second gripper (52), and a connecting shaft (53). The first gripper (51) is fixedly connected to one end of the connecting shaft (53) or integrally formed therewith, the other end of the connecting shaft (53) is fixedly connected to the drive shaft of the drive motor, and one end of the first gripper (51) is pivotally connected to one end of the second gripper (52). The first gripper (51) has a first connecting structure at the other end, and the second gripper (52) has a second connecting structure (521) at the other end. The first connecting structure and the second connecting structure (521) are detachably and fixedly connected, and the culturer (3) is clamped between the first gripper (51) and the second gripper (52).

8. The control method for the cell culture workstation according to claim 7, characterized in that, The clamping assembly (5) further includes a clamping member (54), wherein the first jaw (51) or the second jaw (52) is provided with a threaded hole, and the clamping member (54) is provided with a thread adapted to the threaded hole. The clamping member (54) is installed in the threaded hole, and one end of the clamping member (54) can pass through the threaded hole and abut against the outer wall of the incubator (3); and / or The connecting shaft (53) is horizontally positioned and perpendicular to the axis of the incubator (3).

9. The control method for a cell culture workstation according to any one of claims 1 to 8, characterized in that, The culture device (3) includes a shell and a plurality of hollow fiber tubes installed inside the shell. The interior of the hollow fiber tubes forms a channel for the flow of culture medium, and the cells are attached to the outer wall of the hollow fiber tubes.

10. A cell culture workstation, characterized in that, Includes a controller configured to perform the control method according to any one of claims 1 to 9.

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