Preparation process of standard honeysuckle decoction

Abstract

The invention discloses a preparation process of a honeysuckle standard decoction, and relates to the technical field of traditional Chinese medicine decoction preparation. The preparation method comprises the following steps: firstly, weighing a proper amount of honeysuckle decoction pieces, putting the honeysuckle decoction pieces into a marmite, decocting twice, adding 12 times of water for soaking for 60 minutes for the first time, boiling with strong fire, and decocting with slow fire for 20 minutes; adding 10 times of water for the second time, boiling with strong fire, then decocting with slow fire for 15 minutes, filtering the liquid medicine with 200 meshes, cooling the filtrate to room temperature in cold water bath, combining the two filtrates, performing vacuum concentration at the temperature of 50 DEG C until the weight ratio of the medicinal material to the fluid extract is about 1: 1, collecting the concentrated solution, and performing freeze drying to obtain a sample. According to the method, the honeysuckle decoction is taken as a standard, key quality indexes such as the main content, the characteristic spectrum and the extract of the traditional Chinese medicine single variety after the decoction is prepared are stipulated, the transfer rate is investigated, meanwhile, the transfer rate range of the main content of the honeysuckle medicinal material is stipulated, and the effectiveness of the honeysuckle decoction is ensured after the preparation is carried out according to the standard.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine decoction preparation technology, and in particular to the preparation process of honeysuckle standard decoction. Background Technology

[0002] Honeysuckle is a semi-evergreen vine. Its young branches are reddish-brown and densely covered with yellowish-brown, spreading, stiff, rough hairs, glandular hairs, and short soft hairs. The lower parts are often hairless. Honeysuckle is highly adaptable, tolerating a wide range of temperatures. It prefers moist conditions and is tolerant of waterlogging. It requires moist soil and thrives in low light and shady, rainy environments. It grows slowly under strong light. Sandy loam with a thick soil layer is optimal. It can be cultivated on hillsides, terraces, embankments, dikes, and barren hills. Honeysuckle is sweet and cold in nature, clearing heat and detoxifying, reducing inflammation and swelling. It is effective against bacterial dysentery and various suppurative diseases. Traditional Chinese medicine decoctions are a widely used form of medication in clinical practice. Standardized decoctions are single-herb Chinese medicinal decoctions prepared using standardized processes, guided by traditional Chinese medicine theory and based on clinical application, referencing modern extraction methods. They are used for standardized clinical medication to ensure accuracy and consistency of dosage.

[0003] Currently, there are no quality studies or standards for honeysuckle decoctions, making it impossible to measure the quality and efficacy of the decoction after preparation. This also prevents the establishment of quality standards for the final product of honeysuckle decoctions, leaving room for improvement. Therefore, we propose a preparation process for a standard honeysuckle decoction. Summary of the Invention

[0004] The purpose of this invention is to provide a preparation process for a standard honeysuckle decoction to solve the problems mentioned in the background art.

[0005] This invention is achieved through the following technical solution:

[0006] This invention relates to a preparation process for a standard honeysuckle decoction, comprising the following steps:

[0007] S1: Place honeysuckle slices in a clay pot, add 12 times the amount of water, and soak for 60 minutes. Bring to a boil over high heat, then simmer over low heat for 20 minutes to obtain one decoction. Pour out the first decoction.

[0008] S2: Add 10 times the amount of water to the clay pot again, bring to a boil over high heat, then simmer over low heat for 15 minutes to obtain a second decoction. Pour out the second decoction.

[0009] S3: Filter the first and second medicinal solutions obtained from S1 and S2 through a 200-mesh filter, cool the filtrate to room temperature in a cold water bath, and then combine the two filtrates to obtain honeysuckle medicinal solution;

[0010] S4: The obtained honeysuckle extract was concentrated under vacuum at a temperature of 50℃, with an absolute vacuum of 30-40 mbar, until the weight ratio of the medicinal material to the fluid extract was 1:1.

[0011] S5: Based on S4 above, the concentrated liquid is collected and freeze-dried to obtain the standard honeysuckle decoction.

[0012] Concentration and freeze-drying are used to measure the yield and extract indicators of honeysuckle in decoctions.

[0013] In this invention, standard decoctions serve as a "bridge" connecting traditional Chinese medicine decoction pieces and modern Chinese medicine preparations. They provide a reference for controlling the quality of Chinese medicine end products, a tool for standardizing different forms of Chinese medicine administration to ensure the uniformity of quality and the consistency of efficacy, and a reference for evaluating the consistency of product quality from different manufacturers. Therefore, the research on the quality standards of standard decoctions of Chinese medicine decoction pieces will provide a foundation for the formulation of quality standards for all end products derived from decoctions of Chinese medicine decoction pieces.

[0014] The present invention has the following beneficial effects:

[0015] The preparation process of the honeysuckle standard decoction of the present invention uses objective quality standards to measure the component transfer of honeysuckle slices into decoction, and ensures the quality and effectiveness of honeysuckle decoction.

[0016] The "standard" in the honeysuckle standard decoction of this invention mainly covers the representativeness of the raw materials, the consistency of the preparation process with the traditional method, and the rigor of quality control, which basically ensures the stability and uniformity of the efficacy and quality of the honeysuckle prescription. In addition, the honeysuckle standard decoction has not undergone a drying process, thus maintaining the consistency with the traditional decoction in clinical practice to the greatest extent.

[0017] Of course, any product implementing this invention does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0018] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a flowchart illustrating the preparation process of the honeysuckle standard decoction of the present invention.

[0020] Figure 2 This is a characteristic peak comparison spectrum diagram of the present invention;

[0021] Figure 3This is a comparative characteristic chromatogram of the honeysuckle standard decoction of the present invention. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Please refer to Figure 1 As shown, this invention relates to a preparation process for a standard honeysuckle decoction, the method comprising:

[0024] Weigh an appropriate amount of honeysuckle slices, place them in a clay pot, and decoct twice. For the first decoction, add 12 times the amount of water, soak for 60 minutes, bring to a boil over high heat, then simmer over low heat for 20 minutes. For the second decoction, add 10 times the amount of water, bring to a boil over high heat, then simmer over low heat for 15 minutes. Filter the decoction through a 200-mesh filter, cool the filtrate to room temperature in a cold water bath, combine the two filtrates, and concentrate under vacuum at 50°C and an absolute vacuum of 30–40 mbar until the weight ratio of the medicinal material to the fluid extract is approximately 1:1. Collect the concentrated liquid and freeze-dry it to obtain the sample.

[0025] Extract yield: After vacuum concentration of honeysuckle aqueous extract, the concentrate is freeze-dried to dryness, the dried extract powder is collected and weighed, and the extract yield is calculated. The formula for extract yield is as follows:

[0026]

[0027] Ointment yield range: 26%–33%.

[0028] Extractives: Determined by hot extraction method according to the method for determination of alcohol-soluble extractives (Chinese Pharmacopoeia 2020 Edition, Part IV, General Chapter 2201) using ethanol as solvent. The extractives range of the standard decoction is 36%–49%.

[0029] Content determination: Phenolic acids were determined by high performance liquid chromatography (General Chapter 0512, Chinese Pharmacopoeia 2020).

[0030] Chromatographic conditions and system suitability test: Octadecylsilane-bonded silica gel was used as the packing material; acetonitrile was used as mobile phase A, and 0.1% phosphoric acid solution was used as mobile phase B, with gradient elution performed according to the specifications in Table 1; the column temperature was not higher than 25℃; the flow rate was 0.7 ml per minute, and the detection wavelength was 327 nm. The theoretical plate number, calculated based on the chlorogenic acid peak, should not be less than 10,000.

[0031] Table 1 shows the gradient elution conditions and system suitability test.

[0032]

[0033] Preparation of reference solutions: Accurately weigh appropriate amounts of chlorogenic acid reference standard, 3,5-di-O-caffeoylquinic acid reference standard and 4,5-di-O-caffeoylquinic acid reference standard, place them in a brown volumetric flask, and add 75% methanol to prepare a solution containing 0.28 mg, 0.15 mg and 44 μg per ml, respectively.

[0034] Preparation of the test solution: Take an appropriate amount of this product, grind it into a fine powder, take about 0.2g, weigh it accurately, place it in a stoppered conical flask, accurately add 50ml of 75% methanol, weigh it, sonicate it (power 500W, frequency 40kHz) for 30 minutes, cool it, weigh it again, make up the weight loss with 75% methanol, shake it well, filter it, and take the filtrate to obtain the test solution.

[0035] Determination method: Accurately pipette 10 μl of the reference solution and the test solution into the liquid chromatograph and determine the result.

[0036] This product contains chlorogenic acid (C) per 1g. 16 H 18 O9) should be 33.8mg~62.8mg;

[0037] The chlorogenic acid transfer rate of medicinal materials to standard decoction ranged from 22.5% to 41.7%.

[0038] Each 1g contains phenolic acids, primarily chlorogenic acid (C... 16 H 18 O9), 3,5-di-O-caffeoylquinic acid (C 25 H 24 O 12 ) and 4,5-di-O-caffeoylquinic acid (C 25 H 24 O 12 The total amount should be between 45.6 mg and 84.6 mg.

[0039] The transfer rate of total phenolic acid content from medicinal materials to standard decoction ranged from 24.1% to 47.7%.

[0040] Luteolin was determined by high performance liquid chromatography (General Chapter 0512, Chinese Pharmacopoeia 2020).

[0041] Chromatographic conditions and system suitability test: Phenylsilane-bonded silica gel was used as the packing material (column length 250 mm, inner diameter 4.6 mm, particle size 5 μm); acetonitrile was used as mobile phase A, and 0.3% phosphoric acid solution was used as mobile phase B, with gradient elution performed according to the specifications in Table 2; the detection wavelength was 350 nm. The theoretical plate number, calculated based on the luteolin peak, should be no less than 20,000.

[0042] Table 2 shows the gradient elution conditions and system suitability test.

[0043]

[0044] Preparation of reference solution: Take an appropriate amount of luteolin reference standard, accurately weigh it, and add 70% ethanol to prepare a solution containing 20 μg per ml.

[0045] Preparation of the test solution: Take this product, grind it into a fine powder, take about 0.4 g, accurately weigh it, place it in a stoppered conical flask, accurately add 25 ml of 70% ethanol, weigh it, sonicate it (power 250W, frequency 35kHz) for 30 minutes, cool it, make up the weight loss with 70% ethanol, shake it well, filter it, and take the filtrate to obtain the test solution.

[0046] Determination method: Accurately pipette 10 μl of the reference solution and the test solution into the liquid chromatograph and determine the result.

[0047]

[0048] This product contains 0.62 mg / g to 1.14 mg / g of luteolin per 1g.

[0049] The transfer rate of luteolin content in the decoction of medicinal materials ranged from 24.4% to 45.4%.

[0050] Characteristic chromatogram: determined by high performance liquid chromatography (General Chapter 0512, Chinese Pharmacopoeia 2020).

[0051] Chromatographic conditions and system suitability test: Octadecylsilane-bonded silica gel was used as the packing material (column length 250 mm, inner diameter 4.6 mm, particle size 5 μm); acetonitrile was used as mobile phase A, and 0.4% phosphoric acid solution was used as mobile phase B, with gradient elution performed according to the specifications in Table 3; the flow rate was 1.0 mL / min; the column temperature was 35℃; and the detection wavelength was 350 nm. The theoretical plate number, calculated based on the luteolin peak, should not be less than 2000.

[0052] Table 3 shows the gradient elution conditions and system suitability test.

[0053]

[0054] Preparation of reference solution: Take 1.5g of honeysuckle reference material, place it in a stoppered conical flask, add 50ml of water, heat under reflux for 45 minutes, filter, evaporate the filtrate to dryness, cool, add 50ml of 50% methanol, sonicate (power 250W, frequency 35kHz) for 45 minutes, cool, shake well, filter, and take the filtrate as the reference solution. Separately, take chlorogenic acid reference standard, luteolin reference standard, and rutin reference standard, place them in a brown volumetric flask, add 50% methanol to prepare a mixed solution containing 40μg of each per ml (store below 10℃).

[0055] Preparation of the test solution: Take an appropriate amount of this product, grind it into a fine powder, take 0.4 g, place it in a stoppered conical flask, add 50 ml of 50% methanol, sonicate (power 250 W, frequency 35 kHz) for 45 minutes, remove, cool, filter, and take the filtrate to obtain the test solution.

[0056] like Figure 2 (Peak 2 (S): chlorogenic acid; Peak 4: rutin; Peak 5: luteolin; each milligram of test sample is approximately equivalent to 0.054 grams of medicinal slices) As shown, the chromatogram of the test sample should show 8 characteristic peaks, which should correspond to the retention times of the 8 characteristic peaks in the chromatogram of the reference medicinal material. Peaks 2, 4, and 5 should correspond to the retention times of the corresponding reference peaks. The peak corresponding to the chlorogenic acid reference peak is the S peak. Calculate the relative retention times of each characteristic peak and the S peak. The relative retention times should be within ±10% of the specified values. The specified values ​​are: 0.58 (peak 1), 1.15 (peak 3), 3.80 (peak 6), 4.02 (peak 7), and 4.64 (peak 8).

[0057] Raw material selection: According to the "Technical Requirements for Quality Control and Standardization of Traditional Chinese Medicine Formula Granules," the samples used in the research should be representative, covering the main producing areas or authentic production areas of the medicinal materials to be harvested, with no fewer than three batches from each producing area. Honeysuckle is a cultivated product, distributed throughout my country. It is mainly produced in Shandong, Hebei, and Henan provinces, where its cultivation has reached a large scale and is the main source of commercial honeysuckle. Based on a comprehensive evaluation of raw material quality, sustainable resource supply, and other factors, the preliminary production areas for raw materials used in large-scale production were determined to be Pingyi, Yishui, and Yinan in Shandong Province, Julu in Hebei Province, and Fengqiu in Henan Province. Ultimately, 15 qualified batches were selected for the research of standard decoctions.

[0058] The study and standardization of extract yield and transfer rate are shown in Table 4.

[0059] Table 4 shows the transfer rate (chlorogenic acid) of 15 batches of honeysuckle standard decoction:

[0060]

[0061]

[0062] Based on the data of chlorogenic acid content in 15 batches of honeysuckle medicinal materials, content in standard decoctions, and yield of extract, the average transfer rate of content in standard decoctions was calculated to be 38.7%.

[0063] The range calculated using the mean plus or minus 3 times the SD is 25.2% to 39.0%.

[0064] The range calculated using 70% to 130% of the mean is 22.5% to 41.7%.

[0065] Based on the actual range of 29.2% to 36.2%;

[0066] The upper and lower limits of the standard decoction transfer rate were set at 22.5% to 41.7%.

[0067] Table 5 is a comparison table of the transfer rate (total phenolic acids) of 15 batches of honeysuckle standard decoction:

[0068]

[0069]

[0070] Based on the data of honeysuckle content (total phenolic acids), standard decoction content, and extract yield of 15 batches of medicinal materials, the average transfer rate of standard decoction content was calculated to be 34.4%.

[0071] The range calculated using the mean plus or minus 3 times the SD is 30.4% to 38.4%; the range calculated using the mean of 70% to 130% is 24.1% to 47.7%; combined with the actual range, it is 32.3% to 36.7%.

[0072] The upper and lower limits of the standard decoction transfer rate were set at 24.1% to 47.7%.

[0073] Table 6 shows the transfer rate (luteolin) of 15 batches of honeysuckle standard decoction:

[0074]

[0075]

[0076] Based on the data of honeysuckle content (luteolin), standard decoction content and extract yield of 15 batches of honeysuckle medicinal materials, the average transfer rate of standard decoction content was calculated to be 58%.

[0077] The range calculated using the mean plus or minus 3 times the SD is 28.5% to 41.3%.

[0078] The range calculated using 70% to 130% of the mean is 24.4% to 45.4%.

[0079] Based on the actual range, it is 31.2% to 38.4%;

[0080] The upper and lower limits of the standard decoction transfer rate were set at 24.4% to 45.4%.

[0081] Content Research and Standard Setting: Modern research shows that the main chemical components of honeysuckle are organic acids (chlorogenic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid), flavonoids (luteolin), iridoid glycosides, triterpenoid saponins, iridoid glycosides, volatile oils, and other components. Among them, phenolic acids and luteolin have antibacterial, antiviral, anti-inflammatory, antipyretic, hepatoprotective, choleretic, hypoglycemic, hypolipidemic, immune-enhancing, antioxidant, antifertility, and hemostatic effects. The 2020 edition of the Chinese Pharmacopoeia lists luteolin as an indicator component for quality control of honeysuckle. Combined with the provisions in the national drug standard YBZ-PFKL-2021073 (content determination) of honeysuckle formula granules, this study selected chlorogenic acid, total phenolic acids (including 3,5-di-O-caffeoylquinic acid and 4,5-di-O-caffeoylquinic acid), and luteolin as the quality control indicator components for standard honeysuckle decoctions.

[0082] Chlorogenic acid, phenolic acids (with chlorogenic acid (C 16 H 18 O9), 3,5-di-O-caffeoylquinic acid (C 25 H 24 O 12 ) and 4,5-di-O-caffeoylquinic acid (C 25 H 24 O 12 ));

[0083] Based on the "Technical Requirements for Quality Control and Standard Formulation of Traditional Chinese Medicine Formula Granules", and combined with the chlorogenic acid data of 15 batches of honeysuckle standard decoction, the limits and upper and lower limits of chlorogenic acid in the standard decoction were calculated.

[0084] The range calculated using the mean plus or minus 3 times the SD is: 41.1 mg to 55.5 mg;

[0085] The range calculated using 70%–130% of the mean is 33.8 mg–62.8 mg.

[0086] Based on the actual range of 45.6mg to 52.8mg, the average value of 70% to 130%, i.e., 33.8mg to 62.8mg, was adopted as the upper and lower limits of the standard decoction content.

[0087] Based on the "Technical Requirements for Quality Control and Standard Formulation of Traditional Chinese Medicine Formula Granules", and combined with the data on the total phenolic acid content of 15 batches of honeysuckle standard decoction, the limits and upper and lower limits of the total phenolic acid content in the standard decoction were calculated.

[0088] The range calculated using the mean plus or minus 3 times the SD is: 52.7 mg to 77.5 mg;

[0089] The range calculated using 70%–130% of the mean is 45.6 mg–84.6 mg.

[0090] Based on the actual range of 60.2mg to 72.5mg, the average value of 70% to 130%, i.e., 45.6mg to 84.6mg, was adopted as the upper and lower limits of the standard decoction content.

[0091] Based on the data of chlorogenic acid content in 15 batches of honeysuckle medicinal materials, content in standard decoctions, and yield of extract, the average transfer rate of content in standard decoctions was calculated to be 38.7%.

[0092] The range calculated using the mean plus or minus 3 times the SD is 25.2% to 39.0%.

[0093] The range calculated using 70% to 130% of the mean is 22.5% to 41.7%.

[0094] Based on the actual range of 29.2% to 36.2%;

[0095] The upper and lower limits of the standard decoction transfer rate were set at 22.5% to 41.7%.

[0096] Based on the data of honeysuckle content (total phenolic acids), standard decoction content and extract yield of 15 batches of medicinal materials, the average transfer rate of standard decoction content was calculated to be 58%.

[0097] The range calculated using the mean plus or minus 3 times the SD is 30.4% to 38.4%.

[0098] The range calculated using 70% to 130% of the mean is 24.1% to 47.7%.

[0099] Based on the actual range, it is 32.3% to 36.7%;

[0100] The upper and lower limits of the standard decoction transfer rate were set at 24.1% to 47.7%.

[0101] Luteolin: Based on the "Technical Requirements for Quality Control and Standard Formulation of Traditional Chinese Medicine Formula Granules", and combined with the data of luteolin in 15 batches of standard honeysuckle decoction, the limits and upper and lower limits of luteolin in standard decoction were calculated.

[0102] The range calculated using the mean plus or minus 3 times the SD is: 0.64 mg to 1.12 mg;

[0103] The range calculated using 70%–130% of the mean is 0.62 mg–1.14 mg.

[0104] Based on the actual range of 0.75mg to 1.00mg, the upper and lower limits of the standard decoction content are adopted as the average value of 70% to 130%, i.e., 0.62mg to 1.14mg.

[0105] Based on the data of honeysuckle content (luteolin), standard decoction content and extract yield of 15 batches of honeysuckle medicinal materials, the average transfer rate of standard decoction content was calculated to be 58%.

[0106] The range calculated using the mean plus or minus 3 times the SD is 28.5% to 41.3%.

[0107] The range calculated using 70% to 130% of the mean is 24.4% to 45.4%.

[0108] Based on the actual range, it is 31.2% to 38.4%;

[0109] The upper and lower limits of the standard decoction transfer rate were set at 24.4% to 45.4%.

[0110] Feature mapping research and standard setting, such as Figure 3 As shown, the chromatogram of the standard honeysuckle decoction with experimental batch number BT(JYH)211001 was selected as the reference chromatogram to establish a control characteristic chromatogram. Based on the data analysis results, eight common peaks were finally selected as characteristic peaks, and luteolin was selected as the reference peak. The relative retention times of the characteristic peaks in the chromatogram were calculated.

[0111] By comparing the chromatogram with that of the reference standard, it was found that among the eight characteristic peaks in the characteristic chromatogram of the honeysuckle standard decoction, peak 2(S) is luteolin. According to the national drug standard YBZ-PFKL-2021073 "Honeysuckle Formula Granules", peak 2(S) is chlorogenic acid, peak 4 is rutin, and peak 5 is luteolin.

[0112] Table 7 shows a comparison of retention times for 15 batches of standard honeysuckle decoction:

[0113]

[0114]

[0115] Table 8 shows the relative peak areas of 15 batches of standard honeysuckle decoctions:

[0116]

[0117]

[0118] The results showed that the characteristic chromatograms of the 15 batches of honeysuckle standard decoction test samples all showed chromatographic peaks corresponding to the retention times of the 8 main characteristic peaks in the reference medicinal material. The relative retention time RSD of peaks 1, 2 to 8 was less than 2%, indicating that the elution time of each peak was consistent among the batches.

[0119] Based on the determination results of 15 batches of honeysuckle standard decoction and the retention time data in the repeatability and precision tests of the methodology, it can be seen that the relative retention times of peaks 1 and 3 are relatively stable, basically within ±2%, while the retention times of peaks 6, 7, and 8 are relatively large, with relative retention times basically within ±6%. Therefore, it is stipulated that the chromatogram of the test sample should show 8 characteristic peaks, and the retention times should correspond to the 8 characteristic peaks in the chromatogram of the reference medicinal material. Among them, peaks 2, 4, and 5 should correspond to the retention times of the corresponding reference peaks. The peak corresponding to the chlorogenic acid reference peak is the S peak. The relative retention times of each characteristic peak and the S peak should be within ±2% of the specified value for peaks 1 and 3, and within ±6% of the specified value for peaks 6, 7, and 8.

[0120] Extractives study and standard setting: Extractives were determined using ethanol as the solvent, following the hot extraction method under the "Determination of Alcohol-Soluble Extractives" section of the Chinese Pharmacopoeia 2020, Part IV, General Chapter 2201. The test results for 15 batches of honeysuckle standard decoction are shown in Table 9.

[0121] Table 9 is a comparison table of the results of extract determination of 15 batches of honeysuckle:

[0122]

[0123]

[0124] The average value of the alcohol-soluble extract from 15 batches of standard decoction was 37.4%.

[0125] The range calculated using the mean plus or minus 3 times the SD is 33.4% to 40.4%.

[0126] The range calculated using 70% to 130% of the mean is 26.2% to 48.6%.

[0127] Based on the actual range, it is 35.7% to 39.7%;

[0128] It is recommended to use 36% to 49% as the upper and lower limits of the standard decoction extract.

[0129] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0130] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to any specific implementation. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. The preparation process of honeysuckle standard decoction, characterized in that, Includes the following steps: S1: Place honeysuckle slices in a clay pot, add 12 times the amount of water, and soak for 60 minutes. Bring to a boil over high heat, then simmer over low heat for 20 minutes to obtain one decoction. Pour out the first decoction. S2: Add 10 times the amount of water to the clay pot again, bring to a boil over high heat, then simmer over low heat for 15 minutes to obtain a second decoction. Pour out the second decoction. S3: Filter the first and second medicinal solutions obtained from S1 and S2 through a 200-mesh filter, cool the filtrate to room temperature in a cold water bath, and then combine the two filtrates to obtain honeysuckle medicinal solution; S4: The obtained honeysuckle liquid is concentrated under vacuum at a temperature of 50℃ until the weight ratio of the medicinal material to the fluid extract is 1:

1. S5: Based on S4 above, the concentrated liquid is collected and freeze-dried to obtain the standard honeysuckle decoction.

2. The preparation process of the honeysuckle standard decoction according to claim 1, characterized in that, In step S4, the honeysuckle liquid obtained is concentrated under vacuum at a temperature of 50°C, with the absolute vacuum degree maintained at 30-40 mbar.

3. The preparation process of the honeysuckle standard decoction according to claim 1, characterized in that, In step S5, concentration and freeze-drying are used to measure the yield and extract indicators of honeysuckle in decoction.

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