Microfluidic sample processing system

Abstract

The invention belongs to the field of medical instruments, and discloses a microfluidic sample treatment system which comprises a turntable assembly, an optical detection assembly, a driving mechanism, a liquid adding assembly, a recycling assembly and a controller. The turntable assembly is provided with a plurality of accommodating positions for accommodating micro-fluidic chips; the optical detection assembly detects operation information of the driving mechanism through the light emitting part and the light receiving part; the driving mechanism drives the turntable, the liquid adding assembly and the recycling assembly; the liquid adding assembly is used for adding a sample into the micro-fluidic chip; the recovery assembly is used for recovering the micro-fluidic chip completing the reaction; the controller is used for controlling operation and data processing of the system. The system can efficiently and automatically process a plurality of micro-fluidic chip samples, and the detection efficiency and accuracy are improved.

Description

Technical Field

[0001] This application relates to the field of medical devices, specifically a microfluidic sample processing system. Background Technology

[0002] In the biomedical field, sample testing and analysis are an indispensable part of scientific research and industrial production. Traditional sample testing methods often rely on manual operation, which is not only time-consuming and labor-intensive, but also easily affected by human factors, making it difficult to guarantee the accuracy and repeatability of the test results.

[0003] In recent years, the development of microfluidic technology has provided an effective way to solve the above problems. As a novel miniature experimental platform, microfluidic chips can manipulate fluids at the micrometer scale, enabling the integration, automation, and miniaturization of sample pretreatment, reaction, separation, and detection steps. Microfluidic chips have advantages such as small size, low sample consumption, fast detection speed, high sensitivity, and ease of integration, and therefore have been widely used in biomedicine, environmental monitoring, food safety, and other fields.

[0004] However, existing microfluidic sample processing systems still have some shortcomings. For example, some systems still require considerable manual intervention and cannot achieve full automation; the detection accuracy and repeatability of some systems need to be improved; and some systems have limited sample processing capacity, which cannot meet the needs of large-scale detection.

[0005] Therefore, developing a system that can efficiently and automatically process multiple microfluidic chip samples to improve detection efficiency and accuracy has become an urgent need in the field of microfluidics technology. Summary of the Invention

[0006] To address the aforementioned issues, this application provides a microfluidic sample processing system that integrates modules such as a turntable assembly, an optical detection assembly, a drive mechanism, a liquid addition assembly, a recovery assembly, and a controller to achieve automated sample processing, accurate detection, and efficient management, thereby meeting the high requirements for sample detection in the biomedical field.

[0007] To achieve the above objectives, this application adopts the following technical solution:

[0008] In a first aspect, this application provides a microfluidic sample processing system, including a turntable assembly, an optical detection assembly, a drive mechanism, a microfluidic chip, a liquid addition assembly, and a recovery assembly.

[0009] The turntable assembly includes an upper turntable plate, a lower turntable plate, and a base plate; the upper turntable plate is provided with multiple accommodating positions evenly distributed along the circumferential direction, each accommodating position being used to accommodate a microfluidic chip.

[0010] The optical detection component works in conjunction with the driving mechanism. The optical detection component includes a light emitter and a light receiver, which are arranged opposite to each other.

[0011] The drive mechanism is used to drive the upper plate of the turntable, the microfluidic chip, the liquid addition assembly, and the recovery assembly.

[0012] The liquid addition assembly is used to add the sample into the microfluidic chip.

[0013] The recovery component is used to recover the microfluidic chip after the reaction has completed.

[0014] It also includes a controller, which is electrically connected to the optical detection components and the drive mechanism, respectively.

[0015] In some implementations, the turntable assembly comprises, from bottom to top, a base plate, a lower turntable plate, and an upper turntable plate. A first motor is mounted below the base plate, and the output shaft of the first motor is fixedly connected to a first main shaft. The first main shaft passes through the base plate and the lower turntable plate in sequence, and is fixedly connected to the upper turntable plate.

[0016] In some implementations, a first indexing plate and a first photoelectric sensor are also provided below the base plate.

[0017] In some implementations, the upper plate of the turntable is provided with multiple accommodating positions for placing microfluidic chips. A chip mounting plate is provided in each accommodating position. A second motor and a fifth photoelectric sensor are provided below the chip mounting plate. The chip mounting plate is connected to the output shaft of the second motor, and the bottom of the second motor is fixedly connected to the lower plate of the turntable.

[0018] In some implementations, a boss is provided in the middle of the chip mounting board.

[0019] In some implementations, a second indexing plate and a second photoelectric sensor are also provided below the upper plate of the turntable.

[0020] In some implementations, a first motor drives the upper plate of the turntable to rotate; a second motor drives the chip mounting plate to rotate.

[0021] In some implementations, the lower plate of the turntable is fixed to the upper plate of the turntable by a second support rod.

[0022] In some implementations, the number of second support rods is four.

[0023] In some embodiments, a first support plate is provided between the lower plate of the turntable and the base plate, and the first support plate is fixed to the base plate by first support rods. There are three first support rods. The first support plate has through holes.

[0024] In some implementations, the first motor drives the upper plate and lower plate of the turntable to rotate, while the base plate and the first support plate are not driven by the first motor.

[0025] In some implementations, a lower bearing for the first spindle is provided between the first spindle and the base plate, and an upper bearing for the first spindle is provided between the first spindle and the first support plate. A slip ring is fitted around the first spindle.

[0026] In some embodiments, the liquid dispensing assembly includes a third motor frame. The portion of the first support plate extending out of the lower plate of the turntable is provided with the third motor frame, and the third motor is fixed on the third motor frame. The first support plate is also provided with a liquid dispensing head support plate, which is perpendicular to the first support plate. The side of the first support plate facing the lower plate of the turntable is provided with a first fixing member and a second fixing member. The first fixing member is located above the first support plate, and the second fixing member is located below the first fixing member. The output shaft of the third motor is provided with a first pulley, and the second fixing member is provided below the second fixing member. The first pulley and the second pulley are connected by a first belt. A liquid dispensing head seat is provided above the second fixing member, and a liquid dispensing head is fixed on the liquid dispensing head seat.

[0027] In some implementations, a liquid inlet is located below the liquid inlet head, and this inlet mates with a liquid inlet hole on the microfluidic chip. The number of liquid inlets is six.

[0028] In some implementations, the filling port is a Luer connector.

[0029] In some implementations, a third photoelectric sensor is fixedly mounted on the first fixture.

[0030] In some implementations, a pressure rod is provided below the liquid filling head seat, and a spring is provided inside the pressure rod.

[0031] In some implementations, the recycling assembly includes a fourth motor frame fixed below the first support plate, a fourth motor fixed on the fourth motor frame, and the output shaft of the fourth motor pointing vertically upward and connected to the rotating plate via a second main shaft.

[0032] In some implementations, a third fixing member is provided on the rotating plate, a fifth motor is provided on the top of the third fixing member, the output shaft of the fifth motor extends downward, a push rod is fixedly connected to the far end of the third fixing member, a fourth fixing member is sleeved on the outside of the push rod, a positioning post is provided below the fourth fixing member, the fourth fixing member is fixedly connected to the fifth fixing member, and the fifth fixing member is fixedly connected to the output shaft of the fifth motor.

[0033] In some implementations, a fourth photoelectric sensor is provided on the third fixing member, and a fourth positioning strip is provided on the fifth fixing member. The fourth positioning strip is used in conjunction with the fourth photoelectric sensor.

[0034] In some implementations, the recycling assembly also includes a recycling port located on one side of the rotating base.

[0035] In some implementations, the microfluidic chip includes a chip body, a sealing film, and a waste liquid container. The upper surface of the chip body is provided with reagent holes and sample slots, and the reagent holes and sample slots are through holes.

[0036] In some implementations, six reagent wells and one sample well are grouped together, for a total of six groups. Each sample well is equipped with a filter membrane.

[0037] In some implementations, the microfluidic chip also has positioning holes that mate with the protrusions on the chip mounting plate.

[0038] In some implementations, the reagent orifice includes a first reagent driving orifice, a second reagent driving orifice, a third reagent driving orifice, a fourth reagent driving orifice, a fifth reagent driving orifice, and a sixth reagent driving orifice. The lower surface of the chip body is provided with a first flow channel, a second flow channel, a third flow channel, a fourth flow channel, a fifth flow channel, a sixth flow channel, a light-emitting film orifice, and a waste liquid outflow orifice. The first flow channel is located on the far right, and from right to left, the first, third, fifth, sixth, fourth, and second flow channels are arranged sequentially. All flow channels communicate with the light-emitting film orifice, and a first micro-one-way valve is provided at each contact point between the flow channel and the light-emitting film orifice. The light-emitting film orifice is connected to the waste liquid outflow orifice, and a second micro-one-way valve is provided at the contact point between the light-emitting film orifice and the waste liquid outflow orifice. The opening pressure of the second micro-one-way valve is greater than that of the first micro-one-way valve.

[0039] In some implementations, the distal end of the first flow channel is provided with a first reagent driving orifice, the distal end of the third flow channel is provided with a third reagent driving orifice, and the third flow channel is also provided with a sample slot orifice and a sample temporary storage slot. The sample slot orifice is located between the third reagent driving orifice and the sample temporary storage slot. The distal end of the fifth flow channel is provided with a fifth reagent driving orifice. The distal end of the sixth flow channel is provided with a sixth reagent driving orifice. The distal end of the fourth flow channel is provided with a fourth reagent driving orifice.

[0040] In some implementations, the second reagent drive hole, the fourth reagent drive hole, and the sixth reagent drive hole are located on the same straight line.

[0041] In some implementations, the first reagent driving hole, the third reagent driving hole, and the fifth reagent driving hole are located on the same straight line.

[0042] In some implementations, the second flow channel is the same length as the first flow channel and is the shortest, while the sixth flow channel is the same length as the fifth flow channel and is the longest.

[0043] In some embodiments, the sealing film covers the lower surface of the chip body, and the sealing film has waste liquid holes that are connected to waste liquid outflow holes on the chip body.

[0044] In some implementations, the lower surface of the seal is a waste liquid container.

[0045] The beneficial effects of this application are:

[0046] This application's multi-position design of the turntable assembly enables the system to process multiple samples simultaneously, significantly improving sample processing efficiency. The optical detection component, working in conjunction with the drive mechanism, can monitor and provide real-time feedback on the turntable's rotation angle and position, ensuring precise positioning and operation of the microfluidic chip. The automated design of the liquid addition and recovery components reduces manual intervention, lowers operational errors, and enhances the system's automation level. Based on feedback from the optical detection component, the controller intelligently controls the start and stop of the drive mechanism and analyzes the detection data, achieving intelligent management of the entire system. The flow channel design and one-way valve settings of the microfluidic chip ensure sufficient reaction of reagents on the luminescent membrane, improving detection accuracy and reliability. This application integrates sample processing, liquid addition, detection, and recovery functions, making it suitable for the automated processing of various biological samples and possessing broad application prospects. Attached Figure Description

[0047] Figure 1 This is a three-dimensional schematic diagram of the present application;

[0048] Figure 2 This is a three-dimensional schematic diagram from another perspective of this application;

[0049] Figure 3 This is a top view of this application;

[0050] Figure 4 for Figure 3 Schematic diagram of the DD section;

[0051] Figure 5 This is a rear view of this application;

[0052] Figure 6 This is the right view of this application;

[0053] Figure 7 for Figure 5 Schematic diagram of the BB cross section;

[0054] Figure 8 for Figure 5 Schematic diagram of the CC section;

[0055] Figure 9 This is a schematic diagram of the liquid addition assembly;

[0056] Figure 10 This is a schematic diagram of the recyclable components.

[0057] Figure 11 This is an exploded view of the microfluidic chip structure.

[0058] Figure 12This is a schematic diagram of a cross-section of a microfluidic chip;

[0059] Figure 13 This is a schematic diagram of the lower surface of the chip body;

[0060] Figure 14 for Figure 13 Schematic diagram of the AA section;

[0061] Figure 15 A schematic diagram of one of the microfluidic channel units on the lower surface of the chip body;

[0062] In the picture:

[0063] 101. Upper plate of turntable; 102. Lower plate of turntable; 103. Base plate; 104. Accommodation position; 105. Chip mounting plate; 106. Boss; 107. Chip sensing post; 108. Second support rod; 109. Lower bearing of first spindle; 110. Upper bearing of first spindle; 111. Slip ring; 112. First support plate; 113. First support rod;

[0064] 301. First motor; 302. First spindle; 303. First indexing plate; 304. First photoelectric sensor; 305. Second motor; 306. Fifth photoelectric sensor; 307. Second indexing plate; 308. Second photoelectric sensor; 309. Third motor; 310. Third photoelectric sensor; 311. Fourth motor; 312. Second spindle; 313. Fifth motor; 314. Fourth photoelectric sensor

[0065] 4. Microfluidic chip; 401. Chip body; 402. Sealing membrane; 403. Waste liquid container; 404. Reagent port; 405. Sample slot port; 406. Sample slot; 407. Filter membrane; 408. Positioning hole; 409. First flow channel; 410. Second flow channel; 411. Third flow channel; 412. Fourth flow channel; 413. Fifth flow channel; 414. Sixth flow channel; 415. Light-emitting membrane hole; 416. Waste liquid outlet; 417. First micro check valve; 418. Second micro check valve; 419. Sample storage tank; 420. Waste liquid port;

[0066] 501. Third motor frame; 502. Filler head support plate; 503. First fixing component; 504. Second fixing component; 505. First pulley; 506. Second pulley; 507. First belt; 508. Filler head seat; 509. Filler head; 510. Filler port; 511. Pressure rod

[0067] 601. Fourth motor frame; 602. Rotating plate; 603. Third fixing component; 604. Push rod; 605. Fourth fixing component; 606. Positioning post; 607. Fifth fixing component; 608. Fourth positioning strip; 609. Recycling hole. Detailed Implementation

[0068] To make the objectives, technical solutions, and advantages of this application clearer, the following detailed description is provided in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the scope of this application.

[0069] In the description of this application, it should be understood that the terms "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0070] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0071] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0072] In this application, unless otherwise expressly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0073] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0074] It should also be noted that in the embodiments of this application, the same reference numerals are used to represent the same component or part. For the same part in the embodiments of this application, the reference numerals may only be used to mark one part or part as an example in the figure. It should be understood that the reference numerals are also applicable to other identical parts or parts.

[0075] This application will now be described in detail with reference to the accompanying drawings.

[0076] Please see the appendix Figure 1-8 A microfluidic sample processing system includes a turntable assembly, an optical detection assembly, a drive mechanism, a microfluidic chip 4, a liquid addition assembly, and a recovery assembly.

[0077] The turntable assembly includes an upper turntable plate 101, a lower turntable plate 102, and a base plate 103. The upper turntable plate 101 is provided with a plurality of accommodating positions 104 evenly distributed along the circumferential direction, and each accommodating position 104 is used to accommodate a microfluidic chip 4.

[0078] The optical detection component works in conjunction with the drive mechanism. The optical detection component includes a light emitter and a light receiver, which are arranged opposite to each other. The light receiver is used to receive the light emitted by the light emitter and obtain relevant information about the operation of the drive mechanism by detecting changes in the light.

[0079] The drive mechanism is used to drive the upper plate 101 of the turntable, the microfluidic chip 4, the liquid addition component, and the recovery component to achieve the orderly operation of the entire system.

[0080] The liquid addition component is used to add the sample into the microfluidic chip 4.

[0081] The recovery component is used to recover the microfluidic chip 4 after the reaction has completed.

[0082] It also includes a controller, which is electrically connected to the optical detection component and the drive mechanism. The controller is used to control the start and stop of the drive mechanism and to analyze the detection data based on the feedback information from the optical detection component, thereby realizing intelligent control and management of the entire microfluidic sample processing system.

[0083] Specifically, the turntable assembly consists of a base plate 103, a lower turntable plate 102, and an upper turntable plate 101, arranged from bottom to top. A first motor 301 is installed below the base plate 103. The output shaft of the first motor 301 is fixedly connected to a first spindle 302. The first spindle 302 passes through the base plate 103 and the lower turntable plate 102, and is fixedly connected to the upper turntable plate 101, thereby driving the upper turntable plate 101 to rotate according to a preset program.

[0084] To ensure that the upper plate 101 of the turntable can rotate to a specified angle, a first indexing plate 303 and a first photoelectric sensor 304 are also provided below the base plate 103. The first indexing plate 303 is used to mark the reference position for rotation, and the first photoelectric sensor 304 detects the mark on the indexing plate to provide real-time feedback on the rotation angle and position information of the turntable, thereby realizing the control of the rotation angle of the upper plate 101 of the turntable.

[0085] The upper plate 101 of the turntable is provided with multiple accommodating positions 104 for placing microfluidic chips 4. A chip mounting plate 105 is provided in the accommodating position 104. A second motor 305 and a fifth photoelectric sensor 306 are provided below the chip mounting plate 105. The chip mounting plate 105 is connected to the output shaft of the second motor 305. The bottom of the second motor 305 is fixedly connected to the lower plate 102 of the turntable.

[0086] A protrusion 106 is provided in the middle of the chip mounting plate 105. When the microfluidic chip 4 is placed on the chip mounting plate 105, the fifth photoelectric sensor 306 is identified by the chip sensing post 107.

[0087] Below the upper plate 101 of the turntable, there is also a second indexing plate 307 and a second photoelectric sensor 308. The second indexing plate 307 is used to mark the reference position for the rotation of the microfluidic chip 4, and the second photoelectric sensor 308 detects the mark on the indexing plate to provide real-time feedback on the rotation angle and position information, thereby realizing the control of the rotation angle of the microfluidic chip 4.

[0088] In this application, the first motor 301 drives the upper plate 101 of the turntable to rotate, thereby realizing the switching of each accommodating position 104 of the microfluidic chip 4; the second motor 305 drives the chip mounting plate 105 to rotate, thereby realizing the switching of the liquid filling holes on the microfluidic chip 4.

[0089] The lower plate 102 and the upper plate 101 of the turntable are fixed together by a second support rod 108. In a preferred embodiment of this application, there are four second support rods 108.

[0090] A first support plate 112 is provided between the lower plate 102 of the turntable and the base plate 103. The first support plate 112 and the base plate 103 are fixed by first support rods 113. In a preferred embodiment of this application, the number of first support rods 113 is three. The first support plate 112 is provided with through holes to facilitate the passage of the output shaft of the first motor 301.

[0091] In one embodiment of this application, the first motor 301 drives the upper plate 101 and the lower plate 102 of the turntable to rotate, while the base plate 103 and the first support plate 112 are not driven by the first motor 301. To ensure smooth rotation, a lower bearing 109 of the first spindle 302 is provided between the first spindle 302 and the base plate 103, and an upper bearing 110 of the first spindle 302 is provided between the first spindle 302 and the first support plate 112. In addition, a slip ring 111 is fitted around the first spindle 302 to prevent the connecting wire between the second motor 305 and the photoelectric sensor from getting tangled during rotation.

[0092] After the system starts, the first motor 301 drives the upper plate 101 of the turntable to rotate, realizing the switching between different accommodating positions 104 and moving the microfluidic chip 4 to be processed to the designated processing area. The second motor 305 drives the chip mounting plate 105 to rotate, realizing the switching of the liquid filling holes on the microfluidic chip 4.

[0093] like Figure 9 As shown, the liquid addition assembly includes a third motor frame 501. The portion of the first support plate 112 extending out of the lower plate 102 of the turntable is provided with the third motor frame 501. The third motor 309 is fixed on the third motor frame 501. The first support plate 112 is also provided with a liquid addition head 509 support plate 502, which is perpendicular to the first support plate 112. The side of the first support plate 112 facing the lower plate 102 of the turntable is provided with a first fixing member 503 and a second fixing member 504. The first fixing member 503 is located at... The upper part of the first support plate 112, the second fixing member 504 is located below the first fixing member 503, the output shaft of the third motor 309 is provided with a first pulley 505, the second fixing member 504 is provided with a second pulley 506, the first pulley 505 and the second pulley 506 are connected by a first belt 507, the upper part of the second fixing member 504 is provided with a liquid filling head 509 seat 508, and a liquid filling head 509 is fixed on the liquid filling head 509 seat 508, so that the third motor 309 drives the liquid filling head 509 to move up and down.

[0094] Furthermore, a liquid inlet 510 is located below the liquid inlet head 509 seat 508, and the liquid inlet 510 mates with the liquid inlet hole on the microfluidic chip 4. In a preferred embodiment of this application, the number of liquid inlets 510 is 6. Further, the liquid inlets 510 are Luer connectors.

[0095] A third photoelectric sensor 310 is fixedly installed on the first fixing member 503 to detect whether the liquid filling head 509 seat 508 has moved into place.

[0096] To prevent the microfluidic chip 4 from being lifted when the liquid dispensing head 509 moves upward, a pressure rod 511 is provided below the liquid dispensing head 509 base 508. The pressure rod 511 contains a spring for fixing the chip.

[0097] Once the first motor 301 drives the microfluidic chip 4 to rotate into position, the third motor 309 moves the dispensing head 509 downwards and inserts it into the reagent port 404. The dispensing head 509 is connected to a micro-pump via tubing to achieve precise reagent dispensing.

[0098] like Figure 10 As shown, the recycling assembly includes a fourth motor frame 601, which is fixed below the first support plate 112. A fourth motor 311 is fixed on the fourth motor frame 601. The output shaft of the fourth motor 311 is vertically upward and connected to the rotating plate 602 through the second main shaft 312, so that the fourth motor 311 can drive the rotating plate 602 to rotate.

[0099] A third fixing member 603 is provided on the rotating plate 602. A fifth motor 313 is provided on the top of the third fixing member 603. The output shaft of the fifth motor 313 extends downward. A push rod 604 is fixedly connected to the far end of the third fixing member 603. A fourth fixing member 605 is sleeved on the outside of the push rod 604. A positioning post 606 is provided below the fourth fixing member 605. The fourth fixing member 605 is fixedly connected to the fifth fixing member 607. The fifth fixing member 607 is fixedly connected to the output shaft of the fifth motor 313. Thus, the fifth motor 313 can drive the positioning post 606 to move up and down. To ensure that the movement is in place, a fourth photoelectric sensor 314 is provided on the third fixing member 603. A fourth positioning strip 608 is provided on the fifth fixing member 607. The fourth positioning strip 608 and the fourth photoelectric sensor 314 are used in conjunction to ensure that the movement is in place.

[0100] The recycling assembly also includes a recycling hole 609, which is located on one side of the rotating seat.

[0101] The specific workflow is as follows: After the microfluidic chip 4 completes its reaction, the fifth motor 313 drives the positioning post 606 to move downwards and insert into the liquid inlet of the microfluidic chip 4, and then moves upwards to lift the microfluidic chip 4. Next, the fourth motor 311 drives the rotating seat to rotate, rotating the microfluidic chip 4 above the recovery hole 609. At this time, the fifth motor 313 continues to drive the microfluidic chip 4 to move upwards. When the microfluidic chip 4 contacts the push rod 604, the microfluidic chip 4 detaches from the positioning post 606 and falls into the recovery hole 609, completing the recovery operation.

[0102] like Figure 11-15 As shown, the microfluidic chip 4 includes a chip body 401, a sealing film 402, and a waste liquid box 403. The upper surface of the chip body 401 is provided with a reagent hole 404 and a sample slot hole 405, and the reagent hole 404 and the sample slot hole 405 are through holes.

[0103] In a preferred embodiment of this application, six reagent wells 404 and one sample well 406 form a group, for a total of six groups.

[0104] The sample slot 406 is equipped with a filter membrane 407 for sample pretreatment. The microfluidic chip 4 is also provided with a positioning hole 408 that mates with the protrusion 106 of the chip mounting plate 105 to ensure that the microfluidic chip 4 is placed in place.

[0105] The reagent orifice 404 includes a first reagent driving orifice, a second reagent driving orifice, a third reagent driving orifice, a fourth reagent driving orifice, a fifth reagent driving orifice, and a sixth reagent driving orifice. The lower surface of the chip body 401 is provided with a first flow channel 409, a second flow channel 410, a third flow channel 411, a fourth flow channel 412, a fifth flow channel 413, a sixth flow channel 414, a light-emitting film orifice 415, and a waste liquid outflow orifice 416. Specifically, as shown in the figure, the first flow channel 409 is located on the far right, and from right to left, the first flow channel 409, the third flow channel 411, the fifth flow channel 413, the sixth flow channel 414, the fourth flow channel 412, and the second flow channel 410 are arranged sequentially. All flow channels are connected to the light-emitting film orifice 415, and a first micro one-way valve 417 is provided at each contact point between the flow channel and the light-emitting film orifice 415. The light-emitting film orifice 415 is connected to the waste liquid outflow orifice, and a second micro one-way valve 418 is provided at the contact point between the light-emitting film orifice 415 and the waste liquid outflow orifice. The opening pressure of the second micro check valve 418 is greater than that of the first micro check valve 417 to ensure that the reagent reacts fully on the luminescent membrane.

[0106] The first flow channel 409 has a first reagent driving hole at its distal end, and the third flow channel 411 has a third reagent driving hole at its distal end. The third flow channel 411 also has a sample slot 405 and a sample storage slot 419. The sample slot 405 is located between the third reagent driving hole and the sample storage slot 419. The fifth flow channel 413 has a fifth reagent driving hole at its distal end. The sixth flow channel 414 has a sixth reagent driving hole at its distal end. The fourth flow channel 412 has a fourth reagent driving hole at its distal end.

[0107] It is worth mentioning that the second, fourth, and sixth reagent drive holes are located on the same straight line. The first, third, and fifth reagent drive holes are also located on the same straight line. This facilitates the liquid addition operation.

[0108] In a preferred embodiment of this application, the second flow channel 410 is the same length as the first flow channel 409 and is the shortest, while the sixth flow channel 414 is the same length as the fifth flow channel 413 and is the longest.

[0109] The sealing film 402 covers the lower surface of the chip body 401. The sealing film 402 is provided with a waste liquid hole 420, which is connected to the waste liquid outflow hole 416 on the chip body 401. The lower surface of the sealing film 402 is a waste liquid container 403, and waste liquid can flow into the waste liquid container 403 through the waste liquid outflow hole 416 and the waste liquid hole 420.

[0110] During the operation of the microfluidic chip 4, the sample is first added to the sample slot 406. After being filtered by the filter membrane 407, the sample enters the sample storage slot 419. The reagent port 404 is connected to the Luer connector in the dispensing head 509. A micro air pump precisely controls the flow rate and addition order of the reagents, introducing different reagents into the microfluidic chip 4 sequentially through the reagent port 404. Driven by the micro air pump, the liquid slowly flows through the channels and finally reaches the luminescent membrane. To prevent reagents from flowing back into other channels, a first micro one-way valve 417 is provided in each channel. After passing through the luminescent membrane, the reagent solution flows into the bottom waste liquid box 403 through the waste liquid outlet 416. A second micro one-way valve 418 is provided before flowing into the waste liquid box 403, and the opening pressure of the second micro one-way valve 418 is higher than that of the first micro one-way valve 417, thereby ensuring that the reagent reacts fully on the luminescent membrane. The luminescent membrane is coated with the antigen corresponding to the antibody to be tested. After the reagent reacts with the antigen, a light signal is generated on the luminescent membrane. The CCD camera then reads the luminescence intensity of each reaction point to complete the detection.

[0111] In a preferred embodiment of this application, the system can process six samples simultaneously. Each microfluidic chip 4 is equipped with a unique number, and each sample corresponds to a QR code. After sample addition is completed, relevant information will be automatically entered into the system, achieving efficient sample management and automated operation.

[0112] The above embodiments are merely illustrative of the principles and effects of this application and are not intended to limit this application. Any non-substantial modifications made to this application using this concept shall be considered an infringement of the scope of protection of this application.

Claims

1. A microfluidic sample processing system, characterized in that, include: The turntable assembly includes an upper turntable plate, a lower turntable plate, and a base plate. The upper turntable plate is provided with a plurality of accommodating positions evenly distributed along the circumferential direction, and each accommodating position is used to accommodate a microfluidic chip. An optical detection assembly includes a light emitter and a light receiver, with the light emitter and the light receiver arranged opposite to each other; The drive mechanism includes multiple motors for driving the upper plate of the turntable, the microfluidic chip, the liquid addition assembly, and the recovery assembly; The liquid dispensing assembly, including a third motor and a dispensing head, is used to add samples into the microfluidic chip; The recycling components, including the fourth and fifth motors, are used to recycle the microfluidic chip after the reaction has been completed. The controller is electrically connected to the optical detection components and the drive mechanism, respectively.

2. The microfluidic sample processing system according to claim 1, characterized in that, The turntable assembly also includes: The first motor is installed below the base plate. The output shaft of the first motor is fixedly connected to the first main shaft. The first main shaft passes through the first support plate and the lower plate of the turntable in sequence and is fixedly connected to the upper plate of the turntable. First indexing plate and first photoelectric sensor; A chip mounting board is located within a receiving position. A second motor and a fifth photoelectric sensor are provided below the chip mounting board. The chip mounting board is connected to the output shaft of the second motor, and the bottom of the second motor is fixedly connected to the lower plate of the turntable. The first support plate is located between the lower plate of the turntable and the base plate.

3. The microfluidic sample processing system according to claim 2, characterized in that, A first spindle lower bearing is provided between the first spindle and the base plate, and a first spindle upper bearing is provided between the first spindle and the first support plate; The first spindle sleeve is equipped with a slip ring.

4. The microfluidic sample processing system according to claim 1, characterized in that, The liquid addition assembly includes: The third motor frame is fixed to the part of the first support plate that extends out of the lower plate of the turntable; The third motor is fixed on the third motor frame; The liquid filling head is connected to the third motor via pulleys, belt mechanism, first fixing member, and second fixing member; A liquid filling head seat is fixed above the liquid filling head, and a liquid filling port is provided below the liquid filling head seat.

5. The microfluidic sample processing system according to claim 2, characterized in that, The first support plate has a first fixing member and a second fixing member on the side facing the lower plate of the turntable. The first fixing member is equipped with a third photoelectric sensor. A pressure rod is provided below the liquid filling head seat, and a spring is provided inside the pressure rod.

6. The microfluidic sample processing system according to claim 1, characterized in that, The recycling component also includes: The fourth motor frame is fixed below the first support plate; The fourth motor is fixed on the fourth motor frame, and its output shaft is connected to the rotating plate through the second main shaft; The fifth motor is fixed on the rotating plate, and its output shaft extends downward and is connected to a positioning pin; The fourth photoelectric sensor and the fourth positioning bar; The recycling hole is located on one side of the rotating plate.

7. The microfluidic sample processing system according to claim 1, characterized in that, The microfluidic chip includes: The chip body has reagent holes and sample slots on the upper surface and flow channels, light-emitting film holes and waste liquid outflow holes on the lower surface; A sealing film covers the lower surface of the chip body and has a waste liquid hole connected to a waste liquid outflow hole; Waste liquid container, located on the lower surface of the sealing film.

8. The microfluidic sample processing system according to claim 7, characterized in that, The reagent holes on the upper surface of the chip body include first to sixth reagent driving holes, and the flow channels include first to sixth flow channels. The flow channels are first, third, fifth, sixth, fourth and second flow channels from right to left. All flow channels are connected to the light-emitting film holes, and a first micro one-way valve is provided at the position where each flow channel contacts the light-emitting film hole. The light-emitting membrane hole is connected to the waste liquid outlet hole, and a second micro check valve is provided at the contact position. The opening pressure of the second micro check valve is greater than that of the first micro check valve.

9. The microfluidic sample processing system according to claim 8, characterized in that, The third flow channel is provided with a sample slot and a sample storage slot, and the sample slot is located between the third reagent driving hole and the sample storage slot; the second, fourth and sixth reagent driving holes are located on the same straight line, and the first, third and fifth reagent driving holes are located on the same straight line; The second flow channel is the same length as the first flow channel and is the shortest, while the sixth flow channel is the same length as the fifth flow channel and is the longest.

10. The microfluidic sample processing system according to claim 9, characterized in that, The sample slot is equipped with a filter membrane, the reagent holes and sample slot holes on the upper surface of the chip body are through holes, and the chip is also equipped with positioning holes that cooperate with the protrusions of the chip mounting plate.

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