Weeding composition and application thereof

Abstract

The invention belongs to the technical field of pesticide weeding, and discloses a weeding composition and application thereof.The weeding composition comprises an active component A and an active component B. The active component A is isoxafop-butyl, the active component B is any one of metamifop, cyhalofop-butyl, clodinafop-propargyl and pinoxaden, and the active component B is one of isoxafop-butyl, cyhalofop-butyl, clodinafop-propargyl and pinoxaden. The mass ratio of the active component A to the active component B is (1: 12)-(24: 1). The weeding composition disclosed by the invention is wide in weed control spectrum and can be used for comprehensively preventing and controlling various weeds in rice fields and wheat fields.

Description

[0001] This invention application is a divisional application of application number 202510173689.6, filed on February 18, 2025, entitled "A weeding composition and its application". Technical Field

[0002] This invention belongs to the field of pesticide weeding technology, and discloses a weeding composition and its application. Background Technology

[0003] Metamifop, internationally known as oxazolidinone, chemically named (2R)-2-[4-[(6-chloro-1,3-benzoxazol-2-yl)oxy]phenoxy]-N-(2-fluorophenyl)-N-methylpropionamide, belongs to the aryloxyphenoxypropionate class of herbicides. It inhibits acetyl-CoA carboxylase (ACC) and promotes fatty acid synthesis in plants. It is used as an acetyl-CoA carboxylase inhibitor in foliar application in rice paddies to control various weeds such as barnyard grass, barnyard grass, and Echinochloa crus-galli. The herbicide is absorbed through the stems and leaves and translocated to the growing point via the vascular bundles to achieve its weed-controlling effect.

[0004] Cyhalofop-butyl (D), chemical name: (2R)-2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propionate butyl ester, CAS Registry Number: 122008-85-9. It belongs to the oxyphenoxypropionic acid class of systemic herbicides. It is rapidly translocated within the plant and degrades into a series of metabolites: cyhalofop-butyl (ACID), cyhalofop-methyl (AMIDE), cyhalofop-diol (DIACID), and cyhalofop-butyl (DP), among which ACID is the herbicidal compound. ACID accumulates in the intercalary meristem of weeds, effectively inhibiting acetyl-CoA carboxylase, an enzyme essential for fatty acid biosynthesis, thereby achieving herbicidal effects.

[0005] Clodinafop-propargyl (D), chemical name: (2R)-2-[4-(5-chloro-3-fluoropyridin-2-yl)oxyphenoxy]propionic acid prop-2-ynyl ester, CAS Registry Number: 105512-06-9, belongs to the aryloxyphenoxypropionic acid class of herbicides. It can effectively inhibit the biosynthesis of esters and is an acetyl-CoA hydroxylase inhibitor. It is mainly used to control wild oats, wild oats, oats, ryegrass, common bluegrass, foxtail grass, etc.

[0006] Pinoxaden, internationally known as pinoxaden, chemically named [8-(2,6-diethyl-4-methylphenyl)-7-oxo-1,2,4,5-tetrahydropyrazolo[1,2-d][1,4,5]oxadia-9-yl]2,2-dimethylpropionate, CAS Registry Number: 243973-20-8, is a neophenylpyrazoline herbicide and an acetyl-CoA carboxylase (ACC) inhibitor. It inhibits fatty acid synthesis, halts cell growth and division, and disrupts the lipid structure of the cell membrane, leading to weed death. Pinoxaden is systemic and fast-acting, effectively controlling various grassy weeds in wheat fields, including some difficult-to-control and rapidly spreading species. It is particularly effective against winter wheat weeds such as barnyardgrass, purslane, sedge, wild oats, Japanese wild oats, and variant wild oats, as well as foxtail grass in barley fields.

[0007] Chemical weeding is currently the most effective measure for weed control. However, the long-term and large-scale use of relatively limited chemical herbicides has led to the rapid development of herbicide-resistant weeds in my country's farmland. These weeds are characterized by wide distribution, numerous species, rapid population succession, and high levels of resistance, posing a serious threat to weed control, the ecological environment, and food security in my country's farmland and attracting widespread attention. Summary of the Invention

[0008] The purpose of this invention is to provide a herbicidal composition that is rationally formulated, has low application cost, significant synergistic effect, good herbicidal effect, broad spectrum of herbicides, and is safe for crops.

[0009] Another object of the present invention is to provide a formulation of a herbicidal composition.

[0010] Another object of the present invention is to provide the above composition for the control of weeds in rice paddies or wheat fields.

[0011] To achieve the above objectives, the present invention adopts the following technical solution: a herbicidal composition comprising active ingredient A and active ingredient B, wherein active ingredient A is isoxaflutole, and active ingredient B is any one of oxazolidinyl, cyhalofop-butyl, clodinafop-propargyl, and clodinafop-propargyl, wherein the mass ratio of active ingredient A to active ingredient B is 1:12 to 24:1, or any value within the above range.

[0012] Furthermore, the active ingredient B is oxazolidinyl acetamiprid, and the mass ratio of active ingredient A to active ingredient B is 1:4 to 24:1, or any value within the above range.

[0013] The active ingredient B is cyhalofop-butyl, and the mass ratio of the active ingredient A to the active ingredient B is 1:4 to 24:1, or any value within the above range.

[0014] The active ingredient B is clodinafop-propargyl, and the mass ratio of the active ingredient A to the active ingredient B is 1:6 to 24:1, or any value within the above range.

[0015] The active ingredient B is oxychlorpyrifos, and the mass ratio of active ingredient A to active ingredient B is 1:6 to 16:1, or any value within the above range.

[0016] Furthermore, the active ingredient B is oxazolidinyl acetamiprid, and the mass ratio of active ingredient A to active ingredient B is 1:4 to 16:1, or any value within the above range.

[0017] The active ingredient B is cyhalofop-butyl, and the mass ratio of the active ingredient A to the active ingredient B is 1:4 to 16:1, or any value within the above range.

[0018] The active ingredient B is clodinafop-propargyl, and the mass ratio of the active ingredient A to the active ingredient B is 1:6 to 16:1, or any value within the above range.

[0019] The active ingredient B is oxychlorpyrifos, and the mass ratio of active ingredient A to active ingredient B is 1:3 to 8:1, or any value within the above range.

[0020] Furthermore, the active ingredient accounts for 0.1% to 80% of the total weight of the composition, or any value within the above range.

[0021] Furthermore, the herbicidal composition contains active ingredients and pesticide adjuvants, and can be formulated into any formulation suitable for agricultural use.

[0022] Furthermore, the dosage form of the preparation is a solid dosage form or a liquid dosage form.

[0023] Furthermore, the solid formulation is a wettable powder, and the liquid formulation is an emulsifiable concentrate, a dispersible oil suspension, a microemulsion, an emulsion, or a suspension.

[0024] The present invention also discloses the application of the herbicidal composition described above for controlling unwanted plants.

[0025] Furthermore, the unwanted growing environment is a rice paddy or a wheat field.

[0026] Furthermore, the unwanted plants are grassy weeds, including barnyard grass, Echinochloa crus-galli, Paspalum distichum, Eleusine indica, wild oats, foxtail grass, Alopecurus aequalis, and Poa annua.

[0027] The beneficial effects of this invention are:

[0028] The herbicidal composition of this invention has a reasonable composition, good herbicidal effect, and low application cost. Its herbicidal activity is not a simple additive of active ingredients, but rather has a significant synergistic effect, which can delay the development of herbicide resistance in weeds, is safe for crops, and meets the safety requirements of pesticide formulations. The herbicidal composition of this invention has a very good control effect on grass weeds in rice and wheat fields. Detailed Implementation

[0029] To make the objectives, technical solutions, and advantages of this invention clearer and more concise, the invention is described using the following specific embodiments, but the invention is by no means limited to these embodiments. The embodiments described below are merely preferred embodiments of the invention and can be used to describe the invention, but should not be construed as limiting the scope of the invention. It should be noted that any modifications, equivalent substitutions, and improvements made within the spirit and principles of this invention should be included within the protection scope of this invention.

[0030] The following are preparation methods for formulation examples:

[0031] Emulsifiable concentrate: According to the formula ratio, the measured active ingredients, solvents and co-solvents are added to the mixing tank and stirred to dissolve them. Then, the emulsifier is added, and the remaining solvent is used to make up the balance. The mixture is stirred evenly in the mixing tank and filtered to obtain the emulsifiable concentrate required by the present invention.

[0032] Dispersible oil suspension: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, oil is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the dispersible oil suspension product.

[0033] Microemulsion: According to the formula ratio, the active ingredients, solvents, emulsifiers, etc. are mixed evenly to obtain the oil phase, the antifreeze is mixed evenly with water to obtain the aqueous phase, the oil phase is added to the aqueous phase under stirring and stirred evenly, shearing is continued for 10 minutes, and then the defoamer is added and stirred evenly to obtain small droplets with oil phase particles of 0.01 to 0.1 micrometers, which is the microemulsion product.

[0034] Water-in-oil emulsion: According to the formula ratio, the active ingredients are dissolved in the solvent and emulsifier is added to form a homogeneous oil phase. Deionized water, antifreeze, etc. are mixed together to form a homogeneous aqueous phase. Under high-speed shearing, the oil phase is added to the aqueous phase and sheared until the particle size is qualified. Then, defoamer, thickener, and preservative are added and stirred evenly to form a well-dispersed water-in-oil emulsion product.

[0035] Suspension agent: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension agent product.

[0036] Wettable powder: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, stirred evenly in a mixing tank, and then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0037] Preparation Example 1: 17% isoxaflutole·oxazolidinyl EC (16:1)

[0038] Formula: 16% isoxaflutole, 1% oxazolidinyl sulfadiazine, 30% propylene carbonate, 8% polyoxyethylene sorbitan monooleate, 2% castor oil polyoxyethylene ether, 2% calcium dodecylbenzene sulfonate, and rosin-based vegetable oil to make up the balance.

[0039] Preparation Example 2: 15% Isoxaflutole·Oxazoline dispersible oil suspension (4:1)

[0040] Formula: 12% isoxaflutole, 3% oxazolidinyl sulfonate, 2% lignin sulfonate, 12% tristyrene-phenylphenol polyoxyethylene ether polyoxypropylene ether, 4% Gelbert alcohol polyoxyethylene ether, 2% sodium dodecyl sulfate, 1% silica, 1% organobentonite, 18% 200# solvent oil, methyl oleate to make up the balance.

[0041] Preparation Example 3: 27% isoxaflutole·oxazolidinyl wettable powder (2:1)

[0042] Formula: 18% isoxaflutole, 9% oxazolidinyl sulfonate, 4% succinate sulfonate, 3% naphthalene sulfonate formaldehyde condensate, 2% bleaching powder BX, 5% silica, and kaolin to make up the balance.

[0043] Preparation Example 4: 9% isoxaflutole·cyhalofop-butyl EC (2:1)

[0044] Formula: 6% isoxaflutole, 3% cyhalofop-butyl, 25% acetophenone, 8% ethylene glycol oxyethylene polyoxypropylene ether, 5% phenylethylphenol oxyethylene polyoxypropylene ether, 3% calcium dodecylbenzenesulfonate, methyl oleate to make up the balance.

[0045] Preparation Example 5: 25% Isoxaflutole·Cyflutole Dispersible Oil Suspension (1:4)

[0046] Formula: 5% isoxaflutole, 20% cyhalofop-butyl, 3% fatty alcohol polyoxyethylene ether, 10% alkylphenol formaldehyde resin polyoxyethylene ether, 1% sodium dodecyl sulfate, 1% sodium polycarboxylate, 1% naphthalene sulfonate formaldehyde condensate, soybean oil to make up the balance.

[0047] Preparation Example 6: 25% isoxaflutole·cyhalofop-butyl water-in-oil emulsion (4:1)

[0048] Formula: 20% isoxaflutole, 5% cyhalofop-butyl, 5% xylene, 15% cyclohexanone, 1% BHT, 1% polyoxyethylene sorbitan monooleate, 5% ethylene oxide-propylene oxide copolymer, 5% ethylene glycol, 0.05% silicone defoamer, 0.1% xanthan gum, 0.1% potassium benzoate, deionized water to make up the balance.

[0049] Preparation Example 7: 9% isoxaflutole·cyhalofop-butyl microemulsion (1:2)

[0050] Formula: 3% isoxaflutole, 6% cyhalofop-butyl, 12% xylene, 15% cyclohexanone, 12% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 5% EO-PO block copolymer, 1% fatty alcohol polyoxyethylene ether sodium sulfate, 4% ethylene glycol, 0.05% silicone defoamer, deionized water to make up the balance.

[0051] Preparation Example 8: 34% isoxaflutole·cyhalofop-butyl wettable powder (16:1)

[0052] Formula: 32% isoxaflutole, 2% cyhalofop-butyl, 2% sodium dodecyl sulfate, 3% alkyl naphthalene sulfonate, 5% sodium polynaphthalene sulfonate, 8% kaolin, 10% silica, and bentonite to make up the balance.

[0053] Preparation Example 9: 15% isoxaflutole·clodinafop-propargyl EC (2:1)

[0054] Formula: 10% isoxaflutole, 5% clodinafop-propargyl, 18% propylene glycol methyl ether, 15% ethylene glycol oxyethylene polyoxypropylene ether, 2% sodium dodecyl sulfate, 10% DMF, and methyl oleate to make up the balance.

[0055] Preparation Example 10: 12% Isoxaflutole·Clostridium dichloroisocyanurate dispersible oil suspension (1:3)

[0056] Formula: 3% isoxaflutole, 9% clodinafop-propargyl, 2% phenylethylphenol polyoxyethylene polyoxypropylene ether, 12% styrene-phenol polyoxyethylene ether phosphate, 2% calcium dodecylbenzene sulfonate, 3% succinate sulfonate, 1.5% magnesium aluminum silicate, 1% Tesco 869, corn oil to make up the balance.

[0057] Preparation Example 11: 10% isoxaflutole·clodinafop-propargyl aqueous emulsion (4:1)

[0058] Formula: 8% isoxaflutole, 2% clodinafop-propargyl, 6% sorbitan oleate polyoxyethylene ether, 1% alkylphenol formaldehyde resin polyoxyethylene ether sulfate, 20% cyclohexanone, 0.2% xanthan gum, 5% ethylene glycol, 0.1% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance.

[0059] Preparation Example 12: 14% isoxaflutole·clodinafop-propargyl microemulsion (1:6)

[0060] Formula: 2% isoxaflutole, 12% clodinafop-propargyl, 20% cyclohexanone, 10% guerbert alcohol polyoxyethylene ether, 5% sorbitan oleate polyoxyethylene ether, 1% styrene-phenol polyoxyethylene ether sulfate, 0.1% silicone defoamer, deionized water to make up the balance.

[0061] Preparation Example 13: 25% isoxaflutole·clodinafop-propargyl wettable powder (24:1)

[0062] Formula: 24% isoxaflutole, 1% clodinafop-propargyl, 4% sodium lignosulfonate, 5% sodium alkyl polyoxyethylene ether sulfonate, 2% BX (a type of chemical additive), 5% silica, and kaolin to make up the balance.

[0063] Preparation Example 14: 16% isoxaflutole·clopyralid EC (1:3)

[0064] Formula: 4% isoxaflutole, 12% cyclohexane, 10% dimethyl sulfoxide, 10% isotridecyl alcohol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 18% propylene carbonate, xylene to make up the balance.

[0065] Preparation Example 15: 15% Isoxaflutole·Pyrazosulfuron-methyl dispersible oil suspension (2:3)

[0066] Formula: 6% isoxaflutole, 9% cyclohexane, 1% naphthalenesulfonate formaldehyde condensate, 10% glycerol fatty acid ester polyoxyethylene ether, 5% fatty alcohol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 1% silica, 1% organobentonite, methyl oleate to make up the balance.

[0067] Preparation Example 16: 17% isoxaflutole·clopyralid suspension (16:1)

[0068] Formula: 16% isoxaflutole, 1% cyclophosphamide, 1% fatty alcohol polyoxyethylene ether, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1% naphthalene sulfonate formaldehyde condensate, 4% styrene phenol polyoxyethylene ether phosphate, 1.5% magnesium aluminum silicate, 0.1% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance.

[0069] Indoor joint test

[0070] Example 1: Indoor combined effect test of isoxaflutole with oxazolidinone and cyhalofop-butyl on common weeds in wheat fields

[0071] Test basis: The test was conducted in accordance with NY / T-1155.4-2006 "Guidelines for Indoor Bioassay Tests of Pesticides - Herbicides - Part 4: Activity Assay Tests - Foliar Spray Method".

[0072] Experimental targets: barnyard grass, Echinochloa crus-galli, Paspalum distichum, and Eleusine indica.

[0073] Instruments and equipment: light incubator, quantitative spray equipment, electronic balance, pots and pans, pipettes, etc.;

[0074] Test soil: The test used air-dried loam with organic matter content ≤3%, neutral pH, good air permeability, and sieved.

[0075] Experimental materials: A measured amount of soil was filled to 4 / 5 of the pot, and then watered from the bottom of the pot until the soil was completely saturated. Pretreated weed seeds were evenly sown on the soil surface, covered with 0.5cm–2cm of soil depending on seed size. After sowing, the plants were moved to a greenhouse for conventional cultivation, with watering provided from the bottom. After emergence, thinning was performed to control weed density.

[0076] Test agents: isoxaflutole technical grade, clodinafop-propargyl technical grade, and clodinafop-propargyl technical grade.

[0077] Preparation of the drug: Dissolve the above raw materials in a suitable solvent, and then dilute with a 0.1% Tween 80 aqueous solution.

[0078] Chemical treatment: Foliar spraying was performed according to the experimental design, from low to high doses. Each treatment was replicated four times, with a control group (no chemical treatment) included. After treatment, the surface of the test materials was allowed to air dry naturally before being transferred to a greenhouse for routine cultivation.

[0079] Survey: Weed control activity was investigated and recorded using the absolute value survey method 14 days after treatment.

[0080] Calculation method:

[0081] Fresh weight control efficacy E = (fresh weight of control area - fresh weight of treatment area) × 100 / fresh weight of control area

[0082] The combined effects of the mixtures were determined using the Gowing method, and the calculation formula is as follows:

[0083] Theoretical control efficacy of the mixture E0 = Weed control efficacy of herbicide A at dosage P + Weed control efficacy of herbicide B at dosage Q - Weed control efficacy of herbicide A at dosage P × Weed control efficacy of herbicide B at dosage Q ÷ 100

[0084] Evaluation criteria for the combined effects of mixed drugs:

[0085] E-E0 > 10% indicates a synergistic effect, E-E0 < -10% indicates an antagonistic effect, and E-E0 between ±10% indicates an additive effect.

[0086] Experimental results:

[0087] Table 1. Results of indoor bioactivity tests of isoxaflutole and clodinafop-propargyl combined on barnyardgrass.

[0088]

[0089] Indoor test results showed that the combination of isoxaflutole and clodinafop-propargyl had a synergistic effect on barnyardgrass in the range of mass ratio of 1:6 to 24:1; the synergistic effect on barnyardgrass was most significant when the mass ratio of isoxaflutole to clodinafop-propargyl was 4:1.

[0090] Table 2 Results of indoor bioactivity tests of isoxaflutole and clodinafop-propargyl combined with *Euphorbia lathyris*

[0091]

[0092]

[0093] Indoor test results showed that the combination of isoxaflutole and clodinafop-propargyl had a synergistic effect on Echinochloa crus-galli in the range of mass ratio of 1:12 to 24:1; the synergistic effect was most significant when the mass ratio of isoxaflutole to clodinafop-propargyl was 2:1.

[0094] Table 3. Results of indoor bioactivity tests of isoxaflutole and clodinafop-propargyl combined on Paspalum distichum.

[0095]

[0096]

[0097] Indoor test results showed that the combination of isoxaflutole and clodinafop-propargyl had a synergistic effect on Paspalum distichum in the range of mass ratio of 1:12 to 24:1; the synergistic effect on Paspalum distichum was most significant when the mass ratio of isoxaflutole to clodinafop-propargyl was 2:3.

[0098] Table 4 Results of indoor bioactivity tests of isoxaflutole and clopyralid combined with Eleusine in Eleusine moschata.

[0099]

[0100] Indoor test results showed that the combination of isoxaflutole and clodinafop-propargyl had a synergistic effect on goosegrass in the range of mass ratio of 1:12 to 24:1; the synergistic effect on goosegrass was most significant when the mass ratio of isoxaflutole to clodinafop-propargyl was 1:3.

[0101] Example 2: Indoor combined effect test of isoxaflutole with clodinafop-propargyl and clodinafop-propargyl on common weeds in paddy fields

[0102] Experimental basis: The experiment was conducted in accordance with NY / T 1155.9-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Herbicides Part 9: Herbicide Activity Determination Tests in Paddy Fields - Foliar Spray Method".

[0103] Experimental instruments: light incubator, spray equipment, electronic balance, pipette.

[0104] Target weeds: wild oats, foxtail grass, wild wheatgrass, and annual bluegrass.

[0105] Test agents: isoxaflutole technical grade, oxazolidinyl acetamiprid technical grade, and cyhalofop-butyl technical grade.

[0106] Test soil: The test used sieved, air-dried sandy loam with organic matter content ≤4%, neutral (pH 6.0~8.0), and good permeability.

[0107] Experimental preparation: Fill pots with a measured amount of soil to 4 / 5 full and top-water to fully saturate the soil. Evenly sow the pretreated weed seeds on the soil surface, covering them with 0.5cm–2cm of soil depending on seed size. Keep the soil consistently moist after sowing, then move the pots to a greenhouse for normal management. Cultivate the weeds until they reach the appropriate leaf stage, at which point spraying treatment will be administered.

[0108] Reagent preparation: Dissolve the above test reagents in a suitable solvent and dilute with a 0.1% Tween-80 aqueous solution. Based on the reagent characteristics, set up 5 series of dosages.

[0109] Chemical treatment: Spray treatment was carried out according to the experimental design, from low to high dose. Each treatment was replicated 4 times, and a control group without chemical treatment was included. After treatment, the soil was kept moist, and the plants were moved into the greenhouse for normal management.

[0110] Experimental investigation: Weed growth status was checked and recorded regularly after treatment. The fresh weight of the aboveground parts of each treatment was investigated by absolute value survey method 21 days after treatment.

[0111] Calculation method:

[0112] Fresh weight control efficacy E = (fresh weight of control area - fresh weight of treatment area) × 100 / fresh weight of control area

[0113] The combined effects of the mixtures were determined using the Gowing method, and the calculation formula is as follows:

[0114] Theoretical control efficacy of the mixture E0 = Weed control efficacy of herbicide A at dosage P + Weed control efficacy of herbicide B at dosage Q - Weed control efficacy of herbicide A at dosage P × Weed control efficacy of herbicide B at dosage Q ÷ 100

[0115] Evaluation criteria for the combined effects of mixed drugs:

[0116] E-E0 > 10% indicates a synergistic effect, E-E0 < -10% indicates an antagonistic effect, and E-E0 between ±10% indicates an additive effect.

[0117] Experimental results:

[0118] Table 5 Results of indoor bioactivity tests of isoxaflutole and oxazolidinone in wild oats

[0119]

[0120] Indoor test results showed that the combination of isoxaflutole and oxazolidinone had a synergistic effect on wild oats in the range of mass ratio of 1:4 to 24:1; the synergistic effect on wild oats was most significant when the mass ratio of isoxaflutole to oxazolidinone was 2:1.

[0121] Table 6 Results of indoor bioactivity tests of isoxaflutole and oxazolidinyl sulfadiazine combination on foxtail grass

[0122]

[0123] Indoor test results showed that the combination of isoxaflutole and oxazolidinone had a synergistic effect on foxtail grass in the range of mass ratio of 1:4 to 24:1; the synergistic effect on foxtail grass was most significant when the mass ratio of isoxaflutole to oxazolidinone was 4:1.

[0124] Table 7 Results of indoor bioactivity tests of the combination of isoxaflutole and cyhalofop-butyl on *Alopecurus aequalis*

[0125]

[0126]

[0127] Indoor test results showed that the combination of isoxaflutole and cyhalofop-butyl had a synergistic effect on *Alopecurus aequalis* in the range of mass ratios of 1:4 to 24:1; the synergistic effect was most significant when the mass ratio of isoxaflutole to cyhalofop-butyl was 4:1.

[0128] Table 8 Results of indoor bioactivity tests of isoxaflutole and cyhalofop-butyl combination on Kentucky bluegrass.

[0129]

[0130] Indoor test results showed that the combination of isoxaflutole and cyhalofop-butyl had a synergistic effect on Kentucky bluegrass in the range of mass ratio of 1:4 to 16:1; the synergistic effect on Kentucky bluegrass was most significant when the mass ratio of isoxaflutole to cyhalofop-butyl was 1:2.

[0131] Example 3: Herbicide efficacy test for controlling weeds in wheat fields

[0132] Experimental Site: The experimental site was located in Erpu Village, Banqiao Town, Fengyang County, Anhui Province. The soil fertility was medium to high, and the soil type was yellow-brown loam with a slightly acidic pH. At the time of application, the wheat had 2-3 tillers, and the grass weeds had 1-2 tillers. The main grass weeds in the experimental site were Alopecurus aequalis, Jointed Goatgrass, Leymus chinensis, and Wild Oats, with some Stellaria media and Capsella bursa-pastoris, etc., which were few in number and unevenly distributed.

[0133] Experimental Design: The experiment included 8 treatments, 7 of which were drug-treated and 1 was a blank control. All experimental plots were arranged in a randomized block design, with each treatment replicated 4 times. The plot area was 20 m². 2 .

[0134] Experimental Methods: Foliar spraying was performed using a Biaohe brand electric backpack sprayer. Absolute value surveys were conducted 20 and 40 days after application, with four sampling points per plot, each covering an area of ​​0.25 m². 2 Statistics 1m 2 Calculate the number of weeds remaining in the field and the control efficacy per plant. At 40 days post-application, also measure the fresh weight of various weeds. Calculate the control efficacy per plant and the control efficacy per fresh weight.

[0135] Formula for calculating drug efficacy:

[0136]

[0137] After the application of the pesticide, irregular observations were conducted, and no pesticide damage phenomena such as yellowing leaves, seedling death, or stunted growth were found in any of the wheat plots.

[0138] Experimental results:

[0139] Table 9 Results of herbicide efficacy test for controlling weeds in wheat fields

[0140]

[0141] As shown in Table 9, 20 days after application, the overall plant control efficacy of the combination of isoxaflutole and clodinafop-propargyl was 82.93%–90.60%; the overall plant control efficacy of the combination of isoxaflutole and clodinafop-propargyl was 85.86%–89.69%; and 40 days after application, the overall plant control efficacy of the combination formulation was 86.53%–95.07%, and the overall fresh weight control efficacy was 86.08%–95.04%.

[0142] Example 4: Herbicide efficacy test for controlling weeds in paddy fields

[0143] Experimental Location: This experiment was conducted in Luocheng Town, Wanzai County, Jiangxi Province, a traditional rice-producing area. The experimental fields had good soil and fertilizer conditions, convenient irrigation and drainage, and leveled surfaces. The cultivation and water and fertilizer management conditions in each experimental plot were consistent and consistent with local agricultural production practices.

[0144] Target weeds: mainly barnyard grass and barnyard grass. The density of weeds in paddy fields is relatively high and the distribution is relatively uniform.

[0145] Experimental Design: The experiment consisted of 8 treatments, with 4 replicates per treatment, for a total of 32 plots. The plot area was 20m². 2 Each neighborhood is randomly grouped.

[0146] Application time and method: The experiment was conducted on direct-seeded rice at the 3-5 leaf stage, barnyard grass at the 2-3 leaf stage, and Echinochloa crus-galli at the 2-3 leaf stage. Linong HD-400 backpack sprayer was used for uniform spraying via foliar spraying. The field was drained one day before application and re-irrigated one day after application, maintaining moisture for 7 days. Post-application field management was the same as for other direct-seeded fields.

[0147] Methods: The weed control effects of different treatments on the field were investigated at 15 and 30 days after application. Four points were randomly selected in each plot, with each point measuring 0.25 m. 2 A total of 1m was surveyed. 2 The number of surviving weeds was investigated 15 days after the application, and the control efficacy per plant was calculated. The number of surviving weeds was investigated 30 days after the application, and the control efficacy per plant was calculated. Weeds in the sampling points were pulled out, and their fresh weight was measured to calculate the control efficacy per fresh weight.

[0148] Formula for calculating drug efficacy:

[0149]

[0150] Table 10 Results of herbicide efficacy test for controlling weeds in rice paddies

[0151]

[0152] As shown in the table above, the effective ingredient content of the compound formulations of isoxaflutole + oxazolidinyl and isoxaflutole + cyhalofop-butyl is 70 g / hm. 2 Fifteen days after application, the plant control efficacy against all weeds in paddy fields ranged from 84.84% to 90.92%. Thirty days after application, the comprehensive plant control efficacy and fresh weight control efficacy of the four treatments using the compound formulation were all above 89%, significantly higher than the single-agent control.

[0153] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A herbicidal composition, characterized in that, The herbicidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is isoxaflutole, and active ingredient B is any one of cyhalofop-butyl, clodinafop-propargyl, and cyclonepropalin, and the mass ratio of active ingredient A to active ingredient B is 1:12 to 24:

1.

2. The herbicidal composition according to claim 1, characterized in that, The active ingredient B is cyhalofop-butyl, and the mass ratio of active ingredient A to active ingredient B is 1:4 to 24:

1. The active ingredient B is clodinafop-propargyl, and the mass ratio of active ingredient A to active ingredient B is 1:6 to 24:

1. The active ingredient B is oxychlorpyrifos, and the mass ratio of active ingredient A to active ingredient B is 1:6 to 16:

1.

3. The herbicidal composition according to claim 2, characterized in that, The active ingredient B is cyhalofop-butyl, and the mass ratio of active ingredient A to active ingredient B is 1:4 to 16:

1. The active ingredient B is clodinafop-propargyl, and the mass ratio of active ingredient A to active ingredient B is 1:6 to 16:

1. The active ingredient B is oxychlorpyrifos, and the mass ratio of active ingredient A to active ingredient B is 1:3 to 8:

1.

4. The herbicidal composition according to claim 1, characterized in that, The active ingredient comprises 0.1% to 80% of the total weight of the composition.

5. The herbicidal composition according to claim 1, characterized in that, The herbicidal composition contains active ingredients and pesticide adjuvants, and can be formulated into any formulation suitable for agricultural use.

6. The herbicidal composition according to claim 5, characterized in that, The dosage form of the preparation is either a solid dosage form or a liquid dosage form.

7. The herbicidal composition according to claim 6, characterized in that, The solid formulation is a wettable powder, and the liquid formulation is an emulsifiable concentrate, a dispersible oil suspension, a microemulsion, an emulsion, or a suspension.

8. The application of the herbicidal composition according to any one of claims 1-7 for the control of unwanted plants.

9. The application according to claim 8, characterized in that, The unwanted growing environment is rice paddy or wheat field.

10. The application according to claim 9, characterized in that, The unwanted plants are grassy weeds, including barnyard grass, Echinochloa crus-galli, Paspalum distichum, goosegrass, wild oats, foxtail grass, barnyardgrass, and annual bluegrass.

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