Ultraviolet crosslinking hydrogel for promoting osteogenesis in high-glucose environment and preparation method thereof

Abstract

The invention discloses ultraviolet light cross-linked hydrogel for promoting osteogenesis in a high-glucose environment and a preparation method, and relates to the technical field of bone repair materials, and the key point of the technical scheme is that the ultraviolet light cross-linked hydrogel comprises a matrix material and a photoinitiator, the matrix material comprises methacrylated gelatin, an osteogenesis promoter beta-tricalcium phosphate and melanin nanoparticles, the photoinitiator is lithium phenyl-2, 4, 6-trimethylbenzene sulfonate, the matrix material and the photoinitiator are mixed to obtain a mixture, the mixture is cross-linked under the irradiation condition of ultraviolet light with the wavelength of 405 nm, and the hydrogel is obtained. And forming a three-dimensional network structure which still has the capability of promoting osteogenesis in a high-glucose environment, so as to obtain the ultraviolet crosslinking hydrogel. The stent prepared from the ultraviolet light cross-linked hydrogel has the capabilities of promoting osteogenesis and resisting oxidation in a diabetes mellitus high-glucose environment, and can effectively repair bone defects.

Description

Technical Field

[0001] This invention relates to the field of bone repair materials technology, and more specifically, to a UV-crosslinked hydrogel that promotes osteoogenesis under high sugar conditions and its preparation method. Background Technology

[0002] In diabetic patients, the long-term hyperglycemic environment severely impairs the metabolic and remodeling capabilities of bone tissue, resulting in decreased bone density, osteoporosis, and a significantly increased risk of fractures. This long-term damage to bone structure and function not only increases the burden on patients' lives but also significantly limits the effectiveness of bone defect repair treatments. Existing bone repair methods are generally ineffective in the context of diabetes, mainly because the pathological state of diabetes leads to decreased bone regeneration capacity and continuous deterioration of the local microenvironment. Therefore, the selection and performance optimization of repair materials have become critical issues that urgently need to be addressed.

[0003] Gelatin methacrylate (GelMA) is a photocrosslinkable hydrogel derived from gelatin. It possesses excellent biocompatibility, tunable physicochemical properties, and the ability to promote cell adhesion, making it a popular choice in tissue engineering. However, GelMA exhibits limited osteogenic activity and antioxidant capacity under high-glucose conditions, making it difficult to effectively improve bone repair barriers caused by abnormal glucose metabolism. Therefore, it needs to be used in conjunction with other functional materials to enhance its overall performance.

[0004] β-Tricalcium phosphate (β-TCP) exhibits a rapid degradation rate and stable release of calcium and phosphorus ions, enabling it to participate in bone mineralization and stimulate osteoblast activity, thus providing favorable chemical conditions for bone formation in the local environment. In diabetic patients, β-TCP can alleviate bone repair impairment to some extent, but its use alone is insufficient to address the oxidative stress damage caused by high glucose levels.

[0005] Recent studies have found that the level of reactive oxygen species (ROS) in the body is significantly elevated under long-term high glucose conditions, which is one of the key mechanisms hindering bone defect repair in diabetes. Excessive ROS can induce lipid peroxidation, protein structure damage, and DNA damage, and induce osteoblast apoptosis or differentiation disorders, thereby inhibiting the bone regeneration process. Therefore, reducing ROS levels and alleviating oxidative stress under high glucose conditions is crucial for promoting bone defect repair in diabetes.

[0006] Melanin is a class of naturally occurring macromolecular pigments widely found in living organisms, possessing important biological functions such as free radical scavenging, radiation protection, and regulation of the local microenvironment. Melanin nanoparticles (MNPs) combine the antioxidant activity of natural melanin with the structural advantages of nanomaterials. They can effectively scavenge reactive oxygen species (ROS) through electron transfer and free radical scavenging mechanisms, significantly reducing oxidative stress levels in high-glucose environments, thereby protecting the survival and function of osteoblasts. The nanostructure of MNPs facilitates uniform dispersion in hydrogel systems and enables long-term stable antioxidant effects, giving them the potential to provide sustained protection in materials for repairing bone defects in diabetic patients.

[0007] In summary, for the bone repair impairment caused by long-term hyperglycemia in diabetic patients, the development of a composite hydrogel scaffold suitable for the pathological microenvironment by combining highly efficient osteogenic promoters and anti-ROS functional materials has important research and application value for improving the bone defect repair effect. Summary of the Invention

[0008] The purpose of this invention is to provide an ultraviolet light crosslinked hydrogel that promotes osteogenic formation under high sugar conditions and its preparation method, so as to solve the defects of existing hydrogel scaffolds that lack osteogenic promotion and antioxidant capacity and are difficult to effectively repair bone defects under high sugar conditions in diabetes.

[0009] The above-mentioned technical objective of the present invention is achieved through the following technical solution: a UV-crosslinked hydrogel that promotes osteogenic formation under high sugar conditions, the UV-crosslinked hydrogel comprising a matrix material and a photoinitiator, the matrix material comprising methacrylamide gelatin (GelMA), osteogenic promoter β-tricalcium phosphate (β-TCP), and melanin nanoparticles (MNPs), the photoinitiator being lithium phenyl-2,4,6-trimethylbenzenesulfonate (LAP), the matrix material and the photoinitiator being mixed to obtain a mixture, the mixture being crosslinked under UV irradiation at a wavelength of 405 nm to form a three-dimensional network structure that still has the ability to promote osteogenic formation under high sugar conditions, thus obtaining the UV-crosslinked hydrogel that promotes osteogenic formation under high sugar conditions.

[0010] The present invention is further configured such that the mass concentration of the lithium phenyl-2,4,6-trimethylbenzenesulfonate ranges from 0.2% to 0.3%.

[0011] The present invention is further configured such that: the mass concentration of the methacrylamide gelatin is in the range of 4% to 6%, the mass concentration of β-tricalcium phosphate is in the range of 0.8% to 1.2%, and the mass concentration of the melanin nanoparticles is in the range of 0.8 to 1.2 mg / mL.

[0012] The present invention is further configured such that the average particle size of the melanin nanoparticles ranges from 180 to 210 nm.

[0013] This invention further provides a method for preparing the above-mentioned ultraviolet light crosslinked hydrogel, comprising the following steps:

[0014] S1 Preparation of methacrylamide gelatin solution: Dissolve methacrylamide gelatin in a buffer solution, and control the mass-volume percentage concentration at 4%~6%;

[0015] S2 is added to powdered β-tricalcium phosphate to a final concentration of 0.8%~1.2% by weight and volume, and then ultrasonically dispersed evenly.

[0016] S3 is added to melanin nanoparticles to a final concentration of 0.8~1.2 mg / mL, the average particle size of the melanin nanoparticles is controlled at 130~170 nm, and then mixed well.

[0017] Add photoinitiator lithium phenyl-2,4,6-trimethylbenzenesulfonate to S4 to a final concentration of 0.2%~0.3% by mass and volume, mix thoroughly under light-protected conditions to obtain the reaction solution;

[0018] S5. The reaction solution is placed into a mold and irradiated with ultraviolet light at a wavelength of 405nm and a power of 8-12W for 3-7 minutes to complete cross-linking and obtain a three-dimensionally stable ultraviolet cross-linked hydrogel.

[0019] The present invention is further configured such that the buffer solution includes, but is not limited to, PBS buffer solution.

[0020] The present invention further provides an application of the above-mentioned ultraviolet crosslinked hydrogel: the ultraviolet crosslinked hydrogel is used to prepare an implantable bone scaffold material that promotes bone regeneration and bone repair under high sugar conditions.

[0021] The present invention is further configured such that the ultraviolet cross-linked hydrogel is used to fabricate tissue engineering scaffolds for diabetes-related osteoporosis, fracture repair, and maxillofacial bone defects.

[0022] In summary, the present invention has the following beneficial effects:

[0023] Compared to traditional hydrogel scaffolds without MNPs, the UV-crosslinked hydrogel provided by this invention can effectively remove excess ROS, reduce oxidative stress levels, and protect osteoblast activity in a high-glucose environment.

[0024] The β-TCP in the UV-crosslinked hydrogel releases calcium and phosphorus ions in the hydrogel, promoting bone mineralization and forming a synergistic effect with the antioxidant effect of MNPs, significantly enhancing osteogenic activity.

[0025] The UV-crosslinked GelMA network structure is stable and can simultaneously provide good mechanical support, pore permeability, and adjustable degradation performance, thus achieving continuous support for bone tissue regeneration. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the transmission electron microscopy (SEM) results of the GelMA / β-TCP / MNPs composite hydrogel scaffold prepared in Example 1 of this invention.

[0027] Figure 2 The Fourier transform infrared (FTIR) spectrum of the GelMA / β-TCP / MNPs composite hydrogel scaffold prepared in Example 1 of this invention is shown.

[0028] Figure 3 Figure A shows the CCK8 staining of the four hydrogel scaffolds prepared in Examples 1-4 of this invention; Figure B shows the live and dead cell staining of the four hydrogel scaffolds prepared in Examples 1-4. (HG: high glucose control group, GelMA: pure GelMA hydrogel scaffold, GT: GelMA / β-TCP hydrogel scaffold, GM: GelMA / MNPs hydrogel scaffold, GTM: GelMA / β-TCP / MNPs hydrogel scaffold; Figure B: scale bar 200µm)

[0029] Figure 4 In Figure A, the free radical scavenging ability of the four hydrogel scaffolds prepared in Examples 1-4 of this invention is detected by the DPPH method. Figure 4 Figure B shows the detection of cellular reactive oxygen species scavenging capacity of the four hydrogel scaffolds prepared in Examples 1-4 of this invention. (HG: high glucose control group, GelMA: pure GelMA hydrogel scaffold, GT: GelMA / β-TCP hydrogel scaffold, GM: GelMA / MNPs hydrogel scaffold, GTM: GelMA / β-TCP / MNPs hydrogel scaffold; Figure B: scale bar 200µm)

[0030] Figure 5 In Figure A, the effect of four hydrogel scaffolds prepared in Examples 1-4 of this invention on cell migration ability (scratch test) is shown. Figure 5 In the example B, the effect of the four hydrogel scaffolds prepared in Examples 1-4 of this invention on cell adhesion (F-actin) is shown. Figure 5Figure C shows the effect of the four hydrogel scaffolds prepared in Examples 1-4 of this invention on cell proliferation (Ki-67 immunofluorescence staining). (HG: high glucose control group, GelMA: simple GelMA hydrogel scaffold, GT: GelMA / β-TCP hydrogel scaffold, GM: GelMA / MNPs hydrogel scaffold, GTM: GelMA / β-TCP / MNPs hydrogel scaffold; Figure A: scale bar 200um, Figures B and C: scale bar 50um)

[0031] Figure 6 This image shows immunofluorescence staining of OCN and RUNX2 cells after 7 days of osteogenic induction with the extracts of the four hydrogel scaffolds prepared in Examples 1-4 of this invention. (HG: high glucose control group; GelMA: simple GelMA hydrogel scaffold; GT: GelMA / β-TCP hydrogel scaffold; GM: GelMA / MNPs hydrogel scaffold; GTM: GelMA / β-TCP / MNPs hydrogel scaffold; scale bar 50µm)

[0032] Figure 7 In this invention, the expression of four osteogenic-related genes (OCN, ALP, COL1, and RUNX2) was detected by qPCR after 7, 14, and 21 days of osteogenic induction using extracts from four hydrogel scaffolds prepared in Examples 1-4. (HG: high glucose control group; GelMA: simple GelMA hydrogel scaffold; GT: GelMA / β-TCP hydrogel scaffold; GM: GelMA / MNPs hydrogel scaffold; GTM: GelMA / β-TCP / MNPs hydrogel scaffold) Detailed Implementation

[0033] The following is in conjunction with the appendix Figure 1-7 The present invention will be described in further detail below.

[0034] Example 1: A UV-crosslinked hydrogel that promotes osteoogenesis under high sugar conditions and its preparation method, wherein the UV-crosslinked hydrogel system includes matrix materials methacrylamide gelatin (GelMA), β-tricalcium phosphate (β-TCP), melanin nanoparticles (MNPs) and a photoinitiator.

[0035] In the above-mentioned UV-crosslinked hydrogel system, the mass percentage of GelMA is 4%-6%, the mass percentage of β-TCP is 0.8%-1.2%, the concentration of MNPs is 0.8-1.2 mg / mL, the average particle size is 130-170 nm, and the mass percentage of photoinitiator LAP is 0.2%-0.3%.

[0036] Preparation method:

[0037] S1 Preparation of methacrylamide gelatin solution: Dissolve methacrylamide gelatin in a buffer solution, using PBS buffer at pH 7.4, with a mass-volume percentage concentration controlled between 4% and 6%;

[0038] S2 is added to powdered β-tricalcium phosphate to a final concentration of 0.8%~1.2% by weight and volume, and then ultrasonically dispersed evenly.

[0039] S3 is added to melanin nanoparticles to a final concentration of 0.8~1.2 mg / mL, the average particle size of the melanin nanoparticles is controlled at 130~170 nm, and then mixed well.

[0040] Add photoinitiator lithium phenyl-2,4,6-trimethylbenzenesulfonate to S4 to a final concentration of 0.2%~0.3% by mass and volume, mix thoroughly under light-protected conditions to obtain the reaction solution;

[0041] S5. The reaction solution is placed into a mold and irradiated with ultraviolet light at a wavelength of 405nm and a power of 8-12W for 3-7 minutes to complete cross-linking and obtain a three-dimensionally stable ultraviolet cross-linked hydrogel.

[0042] Example 2

[0043] This embodiment provides a GelMA / β-TCP composite hydrogel without MNPs. The preparation steps are the same as in Example 1, except that the addition of MNPs is omitted in step S3.

[0044] Example 3

[0045] This embodiment provides a GelMA / MNPs composite hydrogel without β-TCP. The preparation steps are the same as in the first embodiment, except that the addition of β-TCP is omitted in step S2.

[0046] Example 4

[0047] This embodiment uses a simple GelMA hydrogel without the addition of β-TCP and MNPs. The remaining preparation steps are the same as in Example 1.

[0048] Example 5 (High Sugar Control)

[0049] To verify the structural characteristics, biocompatibility, antioxidant capacity, and osteopromoting effect of the composite hydrogel described in this invention under high glucose conditions, MC3T3-E1 osteoblasts were selected and cell experiments were conducted under high glucose culture conditions with a glucose concentration of 33.3 mmol / L. The high glucose control group was used without any added materials. The following experiments were conducted using the materials synthesized in Examples 1-4.

[0050] (1) Material structure characterization

[0051] The internal structure of the hydrogel was observed using scanning electron microscopy (SEM). Figure 1 The GelMA / β-TCP / MNPs hydrogel exhibits a uniform and interconnected porous network with a pore size of (103.61±16.90) μm, which is suitable for cell attachment and nutrient delivery.

[0052] The chemical structure of the hydrogel was analyzed using Fourier transform infrared spectroscopy (FTIR). Figure 2 ), approximately 3369 cm -1 A broad peak appears at approximately 2938 cm⁻¹, corresponding to O–H and N–H stretching vibrations, indicating that the amino and hydroxyl structures in the hydrogel molecules are intact. -1 The vibration is C–H stretching; 1655 cm. -1 With 1546 cm -1 These are characteristic peaks of amide I and amide II, respectively, corresponding to C=O stretching and N–H bending of the protein, accompanied by C–N stretching, indicating that the material retains the gelatin backbone structure; 1237 cm⁻¹ -1 With 1334 cm -1 The peak is attributed to amide III and C–N stretching vibrations, reflecting the protein's secondary structure; 1032 cm⁻¹ -1 The peak corresponds to the C–O stretching vibration, which is characteristic of the polyether / hydroxyl groups in the GelMA backbone. The overall spectrum shows that the GelMA hydrogel still retains the characteristic chemical structure of gelatin protein after UV crosslinking, providing a stable organic network basis for subsequent composites of β-TCP and MNPs.

[0053] (2) Biocompatibility

[0054] The viability of MC3T3-E1 cells was detected using the CCK-8 assay. Figure 3 In our study (A), we found that the addition of GelMA, GT, GM, or GHM extracts did not significantly affect the activity of MC3T3 cells, while the cell viability of the GT and GTM groups was slightly higher than that of the control.

[0055] The results of live and dead staining showed that ( Figure 3 One day after adding the scaffold material extract (B), all groups showed uniform green fluorescence in live cells and very little red fluorescence signal in PI, indicating good cell growth and good biocompatibility of the scaffold.

[0056] (3) Antioxidant properties

[0057] The antioxidant capacity of the hydrogels was determined by the DPPH free radical scavenging experiment: the scavenging rate of the GelMA / MNPs and GelMA / β-TCP / MNPs groups containing MNPs was significantly higher than that of the group without MNPs, and the GelMA / β-TCP / MNPs group had the highest rate.

[0058] Intracellular ROS levels were assessed using DCFH-DA staining: the GelMA / β-TCP / MNPs group showed the lowest ROS fluorescence intensity (green), indicating that it had the best ability to clear high glucose-induced oxidative stress.

[0059] (4) Cell function recovery under high glucose environment

[0060] The scratch test results show that ( Figure 5 In group A), the GTM group showed a significant cell migration-promoting effect. At 0 hours after scratch preparation, the initial wound area was essentially the same in all groups. After 24 hours of culture, the wound healing rate in the high-glucose control group was 30.36 ± 1.13%, while the GTM-treated group reached 60.17 ± 4.95%, significantly higher than the control group. Compared with the other three experimental material groups, the GTM group exhibited a superior migration-promoting effect. These data indicate that GTM material can effectively promote cell migration and has potential application value in wound healing.

[0061] Phalloidin staining results showed ( Figure 5 In the GTM group (B group), the F-actin cytoskeleton structure was significantly enhanced compared to the control group. Large, orderly arranged stress fibers were visible in GTM group cells, extending directionally along the cell's long axis and forming dense peripheral bundles. In contrast, the F-actin fibers in the control group cells were thinner, loosely arranged, and some cells exhibited irregular clump distributions. Furthermore, compared to the control group and other material groups, the spreading area of ​​GTM group cells was significantly increased, with more filopodia and lamellar pseudopodia forming at the edges, suggesting potentially enhanced cell adhesion and migration abilities.

[0062] Ki-67 immunofluorescence showed ( Figure 5 In the study of GelMA, GT, GM and GTM groups, Ki67 expression was higher in all groups than in the control group, with GTM expression being the most significant. This indicates that GelMA, GT, GM and GTM groups can all promote proliferation, with GTM group showing the strongest proliferative effect.

[0063] (5) Recovery of osteogenic capacity under high glucose environment

[0064] Immunofluorescence staining detection of Runx2 and OCN proteins ( Figure 6Seven days after osteogenic induction, OCN immunofluorescence results showed that the HG group had only a very weak green fluorescence signal, indicating a low OCN protein expression level; the signal in the GelMA group was slightly enhanced, but the difference was not significant; the OCN fluorescence intensity in the GT and GM groups was further increased compared to the GelMA group, and more diffusely distributed green fluorescence was visible around the cells; the OCN signal in the GTM group was the strongest, with high fluorescence density and a large distribution area, and sufficient overlap with the DAPI-stained nucleus, suggesting that this group significantly promoted the synthesis of cellular matrix proteins in the early stage. RUNX2 immunofluorescence results showed that the green fluorescence signal in the HG group was extremely weak; the GelMA group showed a slight increase but remained low; the RUNX2 signal in the GT and GM groups was further enhanced, especially in the nucleus and surrounding areas; the RUNX2 fluorescence in the GTM group was the strongest, densely distributed and highly overlapping with the DAPI signal, suggesting that it can effectively promote the expression of osteogenic differentiation transcription factors.

[0065] qPCR detection of osteogenic-related genes ALP, COL1, and OCN Figure 7 In the early osteogenic induction phase (7 days), the GTM group showed significantly higher expression levels of four key osteogenic genes (ALP, Runx2, COL1, and OCN) than the high glucose (HG) group and other scaffold groups. ALP expression was increased approximately fourfold, and OCN and COL1 expression were also significantly elevated. The GT and GM groups showed better expression of most genes than the GelMA group, but still lower than the GTM group. These early results suggest that the GTM scaffold can rapidly activate the expression of osteogenic-related genes, potentially promoting early transformation of cells towards osteogenic differentiation. In the intermediate osteogenic induction phase (14 days), the GTM group continued to maintain a significant advantage in OCN and ALP gene expression, and the levels of Runx2 and COL1 were also the highest among all groups. Compared to the early phase, the advantage of the GTM group in COL1 and OCN was more stable, indicating that the scaffold is not only effective in activating genes but also plays a significant role in maintaining high gene expression and promoting intermediate matrix protein production. During the late osteogenic induction phase (21 days), the GTM group maintained the highest expression levels for all genes. COL1 expression was approximately 5-fold higher than in the HG group, and OCN peaked in late mineralization, increasing by approximately 2-3 times compared to the HG group. ALP expression remained significantly higher than all other groups in the late stage, indicating that the GTM scaffold not only promotes early differentiation but also supports long-term, continuous mineralization. The GT and GM groups showed some advantages over the HG and GelMA groups in certain genes, but the magnitude was significantly lower than that of the GTM group.

[0066] Overall, the four key osteogenic-related genes in the GTM group showed a dynamic trend of rapid increase in the early stage, steady increase in the middle stage, and sustained high expression in the late stage, and were significantly higher than those in the high glucose group and other control scaffold groups at each time point. This stable and significantly enhanced expression pattern indicates that the GTM scaffold can effectively activate and maintain the transcription of genes related to osteogenic differentiation and mineralization under high glucose environment, providing long-term support for the osteogenic function of MC3T3 cells.

[0067] Based on the above experimental results, the ultraviolet crosslinked hydrogel of the present invention has significant antioxidant properties and osteopromoting effects under high sugar conditions. It can play a role in the fields of bone defect repair in diabetes, alveolar bone reconstruction and bone tissue engineering scaffolds, and is especially suitable for situations where bone repair capacity is impaired under high sugar conditions.

[0068] This specific embodiment is merely an explanation of the present invention and is not intended to limit the invention. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they are within the scope of the claims of the present invention.

Claims

1. A UV-crosslinked hydrogel that promotes osteogenic formation under high-glucose conditions, characterized in that: The ultraviolet-crosslinked hydrogel comprises a matrix material and a photoinitiator. The matrix material includes methacrylamide gelatin, osteogenic promoter β-tricalcium phosphate, and melanin nanoparticles. The photoinitiator is lithium phenyl-2,4,6-trimethylbenzenesulfonate. The matrix material and the photoinitiator are mixed to obtain a mixture. The mixture is crosslinked under ultraviolet light irradiation at a wavelength of 405 nm to form a three-dimensional network structure that still has the ability to promote osteogenic formation in a high-sugar environment, thus obtaining the ultraviolet-crosslinked hydrogel that promotes osteogenic formation in a high-sugar environment.

2. The ultraviolet-crosslinked hydrogel that promotes osteoogenesis under high sugar conditions according to claim 1, characterized in that: The mass concentration range of the lithium phenyl-2,4,6-trimethylbenzenesulfonate is 0.2% to 0.3%.

3. The ultraviolet-crosslinked hydrogel that promotes osteoogenesis under high sugar conditions according to claim 1, characterized in that: The mass concentration of the methacrylamide gelatin ranges from 4% to 6%, the mass concentration of β-tricalcium phosphate ranges from 0.8% to 1.2%, and the mass concentration of the melanin nanoparticles ranges from 0.8% to 1.2 mg / mL.

4. The ultraviolet-crosslinked hydrogel that promotes osteoogenesis under high sugar conditions according to claim 1, characterized in that: The average particle size of the melanin nanoparticles ranges from 180 to 210 nm.

5. The method for preparing a UV-crosslinked hydrogel that promotes osteoogenesis under high sugar conditions according to claim 1, characterized in that: Includes the following steps: S1 Preparation of methacrylamide gelatin solution: Dissolve methacrylamide gelatin in a buffer solution, and control the mass-volume percentage concentration at 4%~6%; S2 is added to powdered β-tricalcium phosphate to a final concentration of 0.8%~1.2% by weight and volume, and then ultrasonically dispersed evenly. S3 is added to melanin nanoparticles to a final concentration of 0.8~1.2 mg / mL, the average particle size of the melanin nanoparticles is controlled at 130~170 nm, and then mixed well. S4 is mixed with photoinitiator lithium phenyl-2,4,6-trimethylbenzenesulfonate to a final concentration of 0.2%~0.3% by mass and volume, and thoroughly mixed under light-protected conditions to obtain the reaction solution; S5. The reaction solution is placed into a mold and irradiated with ultraviolet light at a wavelength of 405nm and a power of 8-12W for 3-7 minutes to complete cross-linking and obtain a three-dimensionally stable ultraviolet cross-linked hydrogel.

6. The method for preparing a UV-crosslinked hydrogel that promotes osteoogenesis under high sugar conditions according to claim 5, characterized in that: The buffer solution includes, but is not limited to, PBS buffer solution.

7. The application of the ultraviolet-crosslinked hydrogel that promotes osteoogenesis under high sugar conditions according to any one of claims 1-4, characterized in that: The ultraviolet-crosslinked hydrogel is used to prepare an implantable bone scaffold material that promotes bone regeneration and bone repair under high-sugar conditions.

8. The application of the ultraviolet-crosslinked hydrogel that promotes osteoogenesis under high sugar conditions according to any one of claims 1-4, characterized in that: The ultraviolet-crosslinked hydrogel is used to fabricate tissue engineering scaffolds for diabetes-related osteoporosis, fracture repair, and maxillofacial bone defects.

Images