Organic nutrient solution prepared by taking pig manure as basic raw material and application of organic nutrient solution

Abstract

The invention relates to the technical field of resource utilization of manure, in particular to an organic nutrient solution prepared by taking pig manure as a basic raw material and application of the organic nutrient solution. The pig manure organic nutrient solution which is convenient, cost-saving and synergistic is provided by taking sterilized pig manure as a raw material and utilizing micro-aerobic decomposition fermentation and facultative anaerobic synthesis fermentation means, and a high-quality fertilizer resource is provided for agriculture; the organic nutrient solution disclosed by the invention is wide in raw material source, short in fermentation period and capable of increasing the content of organic matters in soil and improving the health of the soil, so that the planting effect of crops is improved, the yield increase of the crops or the improvement of the quality of the crops is promoted, the organic nutrient solution is safe and environment-friendly, meanwhile, the use of chemical fertilizers and chemical pesticides can be reduced, and the fertilization cost is reduced. According to the method, efficient resource utilization of the pig manure is achieved, a new technical path is provided for resource utilization and high-value fermentation of the pig manure and urine, and the method has a wide application prospect.

Description

Technical Field

[0001] This invention relates to the field of manure resource utilization technology, and in particular to an organic nutrient solution made from pig manure as a basic raw material and its application. Background Technology

[0002] Liquid manure (a mixture of manure and urine) produced during pig farming is a highly efficient organic fertilizer. Pig manure and urine contain a certain amount of organic matter, nitrogen, phosphorus, potassium, and other nutrients necessary for plant growth, making them important nutrient resources for plants. Taking fattening pigs as an example, the daily solid manure production during their raising process is 1.17 kg / head / day, and the urine production is 2.87 kg / head / day, with total nitrogen, total phosphorus, and total potassium content of 38.4 g / head / day, 7.29 g / head / day, and 21.8 g / head / day, respectively. Besides the nitrogen, phosphorus, and potassium nutrients that are important for organic fertilizer application, livestock and poultry manure and urine also contain a large amount of semi-decomposed plant-derived organic matter, including proteins (including protein decomposition products), fats, carbohydrates, cellulose, hemicellulose, lignin, organic acids, chlorophyll, enzymes, and various inorganic salts, as well as urea, uric acid, hippuric acid, and water-soluble substances such as potassium, sodium, calcium, and magnesium in the urine. Practice has proven that the combined application of chemical fertilizers and organic fertilizers in agricultural planting plays a significant role in improving soil fertility, regulating soil microbial flora, and preventing soil-borne diseases. The complex organic components in livestock and poultry manure and their effects on planting provide a material basis and application prospect for exploring their biotransformation and synthesis potential and preparing highly efficient organic nutrient liquid fertilizers.

[0003] Currently, large-scale farms mainly use anaerobic fermentation of pig manure for treatment through processes such as returning the biogas slurry to the fields, storing and fermenting it before returning it to the fields, using off-site fermentation beds, and ensuring compliant discharge. Existing methods for treating liquid manure, primarily focused on returning it to the fields, are relatively simple and primarily emphasize the disposal and treatment of manure. They have not yet developed technologies and products that can fully exploit the biotransformation potential of manure and efficiently synthesize it into high-value organic nutrient solutions suitable for crop cultivation.

[0004] Currently, the mainstream liquid manure fermentation and returning technologies to the field mainly include the following two categories: (1) Anaerobic fermentation biogas slurry return to the field technology: After pretreatment such as impurity removal and conditioning, liquid manure enters a closed reaction device, where it is stabilized and rendered harmless under the action of anaerobic microorganisms. The hydraulic retention time for ambient temperature fermentation is usually no less than 8 weeks, and for mesophilic fermentation, it is no less than 3 weeks. The biogas produced during the treatment process can be used as an energy source, while the biogas slurry and biogas residue are used as organic fertilizer (biogas fertilizer). Biogas slurry planned for return to the field should be further treated in open or closed storage facilities for no less than 60 days to ensure full decomposition.

[0005] (2) Storage and fermentation technology for returning to the field: After solid-liquid separation, the manure and urine are stored and fermented in an oxidation pond. If it is a closed storage, the cycle is no less than 90 days; if it is an open storage, the cycle is no less than 180 days. After the manure and urine are fully decomposed, they are directly used in the farmland.

[0006] Current conventional livestock and poultry manure fermentation and return-to-field technologies are guided by harmless treatment standards and conventional nitrogen, phosphorus, and potassium nutrient concentrations for resource utilization. This is disconnected from the needs of farmland soil improvement and high-efficiency planting, severely limiting the high-value utilization and industrial development of livestock and poultry manure. Its main shortcomings are: 1) Long fermentation cycle and high investment and operating costs. The fermentation and storage maturation cycle often takes several months, greatly increasing construction and production costs; 2) The fermentation process is simple, but it is difficult to meet the needs of planting. It mainly relies on simple anaerobic fermentation and lacks efficient organic nutrient solution fermentation production processes based on planting needs, resulting in low product application efficiency; 3) Large amounts of fertilizer are applied to the field, posing potential environmental pollution risks. Based on the application strategy of applying fertilizer according to nitrogen, phosphorus and potassium concentrations, several tons or even more than ten tons are often required per acre of farmland, resulting in high application costs and significant environmental risks. Summary of the Invention

[0007] To overcome the shortcomings of existing methods for the resource utilization and high-value fermentation of pig manure, this invention aims to improve farmland soil health and increase planting efficiency, and provides an organic nutrient solution whose preparation method includes: (1) Using sterilized pig manure as raw material, carbon source and nitrogen source are added, and then EM agent, compound microbial agent and biological enzyme are introduced for micro-aerobic decomposition and fermentation to obtain primary fermentation liquid; the compound microbial agent is composed of compound Bacillus belye, Bacillus subtilis and compound streptococcus; the compound streptococcus is composed of Streptomyces flavus and Streptomyces freundii. (2) Add a nitrogen source to the primary fermentation broth and carry out facultative anaerobic synthetic fermentation to obtain a secondary fermentation broth; (3) The organic nutrient solution is prepared by adding plant growth promoter to the secondary fermentation liquid.

[0008] This invention, based on the composition and physicochemical characteristics of pig manure, employs a biodegradation-synthetic liquid fermentation method. Using liquid pig manure as the main fermentation raw material, after carbon and nitrogen source supplementation, biological enzymes are added, and a specific compound microbial agent is used for micro-aerobic-facultative anaerobic fermentation. Plant growth promoters are then added to the fermentation broth to produce a highly efficient liquid organic nutrient solution. The use of the aforementioned specific compound microbial agent, through synergistic effects, results in metabolic products that increase soil organic matter content, improve soil structure, and enhance soil fertility. The secreted metabolic products' active substances stimulate crop root growth and development, leading to more developed roots with stronger water and nutrient absorption capabilities. Furthermore, it inhibits the reproduction of soil-borne pathogens, reducing plant diseases and thus improving crop yield and quality.

[0009] Preferably, the mass ratio of the compound Bacillus belyi, Bacillus subtilis and the compound streptococcus is (1~2):(0.8~1.2):1, more preferably (1~1.5):(0.8~1.2):1.

[0010] At the above-mentioned mass ratio, the propagation effect of various microorganisms in the same fermentation system reaches a better level, and the types and contents of nutrients produced by metabolism are more sufficient, which is more conducive to improving the crop planting effect.

[0011] Preferably, the composite Bacillus belyssus is composed of Bacillus belyssus BNCC352076 and Bacillus belyssus NM153; more preferably, the mass ratio is 1:(0.8~1.2). And / or, the Bacillus subtilis is Bacillus subtilis BNCC336770; And / or, the pig manure is liquid pig manure after solid-liquid separation, preferably with COD > 5000 mg / L, and preferably the pig manure is filtered through 20-mesh and 40-mesh screens before use; And / or, the sterilization is performed using 1200~1500 watts of ultraviolet light, preferably for 1~2 hours.

[0012] The process involves two stages of filtration to remove large, insoluble impurities while retaining easily fermentable and decomposed organic matter. Ultraviolet sterilization kills harmful pathogenic bacteria and other microorganisms in pig manure and urine, making it more conducive to the growth and reproduction of beneficial microorganisms.

[0013] Preferably, the compound streptococcus is composed of Streptomyces flavus and Streptomyces freundii in a mass ratio of 1:(0.8~1.2).

[0014] In some implementation schemes, the viable count of the compound Bacillus belysium is ≥100 billion / g, the viable count of Bacillus subtilis is ≥100 billion / g, and the viable count of the compound Streptococcus pneumoniae is ≥20 billion / g.

[0015] In specific implementation, the carbon source includes at least one of straw powder, starch, molasses, glucose, and brown sugar; preferably, the molasses includes beet molasses or sugarcane molasses. And / or, the nitrogen source includes at least one of soybean protein powder and corn protein powder.

[0016] The present invention also unexpectedly discovered that, during the facultative anaerobic synthesis fermentation process, the addition of the above-mentioned protein auxiliary nitrogen source can effectively remove the odor during the fermentation of manure, while the effect of removing odor is not obvious when adding other substances such as molasses for facultative anaerobic synthesis fermentation.

[0017] Preferably, the carbon source is a mixture of molasses and straw powder in a mass ratio of (2~4):1.

[0018] Preferably, the nitrogen source is a mixture of soybean protein powder and corn protein powder mixed in a mass ratio of (1~2):1.

[0019] In specific implementation, the bioenzyme includes synthetic bioenzymes and mixed glycoenzymes; The synthetic biological enzymes include proteases, cellulases, and hemicellulases; The mixed glycoenzymes include glucosidase, pectinase, saccharifying enzyme, and chitosanase; And / or, the plant promoters include seaweed extracts and mineral elements.

[0020] Preferably, the bioenzyme comprises a synthetic bioenzyme and a mixed glycoenzyme in a mass ratio of 1:(0.8~1.2); In the synthesized biological enzyme, the mass ratio of protease, cellulase, and hemicellulase is (1.5~2.5):1:(0.3~0.7). In the mixed glycoenzymes, the mass ratio of glucosidase, pectinase, saccharifying enzyme and chitosanase is (1.5~2.5):(0.3~0.7):(0.3~0.7):1.

[0021] Preferably, the enzyme activity of each enzyme in the mixed glycosylase is ≥500 U / g, and / or, the enzyme activity of any one of the protease, cellulase and hemicellulase in the synthetic bioenzyme is ≥1000 U / g.

[0022] Preferably, the plant growth promoter includes seaweed extract and mineral elements.

[0023] Adding plant growth promoters can enhance crops' ability to absorb nutrients and also help improve the physiological activity of beneficial microorganisms. The seaweed extract contained in these promoters is rich in polysaccharides, natural plant growth regulators, amino acids, alginic acid, minerals, vitamins, and unsaturated fatty acids, among other active ingredients. These components work synergistically with various metabolites in the fermentation broth to improve the soil environment, promote plant growth, and thus increase crop yield and quality. Simultaneously, the trace minerals in the promoters work synergistically with the seaweed extract and metabolites in the fermentation broth to further promote crop growth, enhance its resistance to stress, and reduce disease occurrence.

[0024] Preferably, the seaweed extract contains 20% to 30% (w / w) of seaweed polysaccharides.

[0025] Preferably, the mineral elements contain 20%~30% (w / w) calcium, 5%~10% (w / w) magnesium, 10%~15% (w / w) sulfur, 7%~15% (w / w) iron, 2%~5% (w / w) copper, 5%~10% (w / w) manganese, 5%~8% (w / w) zinc and 5%~7% (w / w) boron.

[0026] Preferably, 1-6 kg of seaweed extract and 2-10 kg of mineral elements are added per ton of secondary fermentation liquid.

[0027] Preferably, the mass ratio of sterilized fecal waste, carbon source, and nitrogen source is (20~200):(2~10):1; more preferably, it is (20~190):(2.5~6):1.

[0028] Preferably, in the substrate of the microaerobic decomposition fermentation, the C / N ratio is (20~30):1; in the material of the microaerobic decomposition fermentation, the mass ratio of EM agent, compound microbial agent and biological enzyme is (5~20):(5~15):1; more preferably, it is (5~6):(5~6):1.

[0029] Preferably, 3-15 kg of EM inoculant, 3-10 kg of compound microbial inoculant, and 0.5-2 kg of biological enzymes are added to each ton of substrate for microaerobic decomposition fermentation.

[0030] Preferably, the temperature of the microaerobic decomposition fermentation is 5℃~40℃; the conditions for microaerobic decomposition fermentation include: gas supply, stirring, and maintaining the DO of the fermentation broth at 1.0~2.0 mg / L; the endpoint of the microaerobic decomposition fermentation is a pH below 5.

[0031] More preferably, the temperature of the microaerobic decomposition fermentation is 22℃~32℃.

[0032] The fermentation process parameters of this invention were further optimized, and it was found that fermentation under the above parameter conditions can improve the fermentation effect and shorten the fermentation cycle.

[0033] Preferably, the microaerobic decomposition fermentation time is 2 to 3 days.

[0034] Preferably, 5-30 kg of nitrogen source is added to each ton of the primary fermentation liquid before anaerobic synthesis fermentation is carried out; the temperature of the anaerobic synthesis fermentation is 5℃-40℃; the conditions for anaerobic synthesis fermentation are open storage fermentation without gas supply or stirring; the endpoint of the anaerobic synthesis fermentation is that the fermentation liquid turns golden yellow, has no obvious fecal odor, and has a relatively obvious sweet and sour grain aroma.

[0035] More preferably, the temperature of the facultative anaerobic synthetic fermentation is 24℃~33℃.

[0036] The fermentation process parameters of this invention were further optimized, and it was found that fermentation under the above parameter conditions can improve the fermentation effect and shorten the fermentation cycle.

[0037] Preferably, the facultative anaerobic synthesis fermentation time is 3 to 5 days.

[0038] The fermentation process of this invention adopts ambient temperature fermentation conditions, reducing energy consumption and fermentation costs by lowering the temperature control of conventional fermentation. Compared with the anaerobic fermentation process for producing biogas and biogas slurry and returning it to the field (>70 days) and the manure storage process (>90 days), the production cycle is shortened to 5-7 days, and the investment in production equipment is only one-fifth of that of conventional anaerobic fermentation processes, resulting in low production and operating costs.

[0039] Preferably, the organic nutrient solution is obtained by adding a plant growth promoter to the secondary fermentation liquid and then filtering it through three stages.

[0040] The product undergoes a three-stage filtration system to remove bioflocculents and impurities generated during the fermentation process, making it more suitable for integrated water and fertilizer systems and preventing clogging of drip and sprinkler irrigation devices.

[0041] Most preferably, the preparation method includes: (1) After solid-liquid separation, the pig liquid manure (preferably COD>5000mg / L) is filtered through a 20-mesh sieve and a 40-mesh sieve to remove large particulate impurities; then it is sterilized by ultraviolet light to obtain the manure fermentation stock solution to be prepared. (2) After mixing the manure fermentation liquid with carbon and nitrogen sources, the C / N ratio of the mixture is (24~26):1, and the fermentation substrate is obtained; the carbon source is a mixture of beet molasses and straw powder in a mass ratio of (3~5):1; the nitrogen source is a mixture of soybean protein powder and corn protein powder in a mass ratio of (1.8~2.2):1; the mass ratio of manure fermentation liquid to carbon and nitrogen sources is (18~22):(2~3):1; (3) The fermentation substrate is mixed with EM inoculant, compound microbial inoculant and biological enzyme in a mass ratio of (450~550):(6~6.5):(5~5.5):1, stirred and aerated for microaerobic decomposition fermentation, and fermentation is terminated when the pH value of the fermentation liquid drops below 5 to obtain the primary fermentation liquid; wherein, the compound microbial inoculant is composed of compound Bacillus berreatus, Bacillus subtilis and compound streptococcus in a mass ratio of (1~1.5):(0.8~1.2):1; the compound Bacillus berreatus is composed of Bacillus berreatus BNCC352076 and Bacillus berreatus NM153 in a mass ratio of 1:(0.8~1.2); the Bacillus subtilis is Bacillus subtilis BNCC33 6770; The compound streptococcal bacteria are composed of *Streptomyces flavus* and *Streptomyces freundii* in a mass ratio of 1:(0.8~1.2); the bioenzymes include synthetic bioenzymes and mixed glycoenzymes in a mass ratio of 1:(0.8~1.2); the synthetic bioenzymes include proteases, cellulases, and hemicellulases; the mass ratio of proteases, cellulases, and hemicellulases is (1.5~2.5):1:(0.3~0.7); the mixed glycoenzymes include glucosidase, pectinase, saccharifying enzymes, and chitosanase; the mass ratio of glucosidase, pectinase, saccharifying enzymes, and chitosanase is (1.5~2.5):(0.3~0.7):(0.3~0.7):1; the fermentation conditions are: gas supply, stirring, fermentation broth DO maintained at 1.0~2.0 mg / L; fermentation temperature is 25℃~30℃, fermentation time is 2~3 days; (4) Add 20-22 kg of the nitrogen source to each ton of primary fermentation liquid, stir and mix, and carry out anaerobic synthesis fermentation and remove odor. When the fermentation liquid turns golden yellow, has no obvious fecal odor, and has a relatively obvious sweet and sour grain aroma, stop fermentation to obtain secondary fermentation liquid; the fermentation conditions are open storage fermentation, no gas supply and stirring; the fermentation temperature is 26℃~30℃; the fermentation time is 3~4 days; (5) Add 6-8 kg of seaweed extract and 10-12 kg of mineral element powder to each ton of the secondary fermentation liquid, and then filter through three stages to obtain the organic nutrient solution.

[0042] The organic nutrient solution of this invention meets the nutrient content and harmlessness standards of GB / T40750-2021 Agricultural Biogas Slurry. The product is rich in nutrients such as amino acids, organic acids, polysaccharides, and polypeptides. Field experiments on cotton have verified that the organic nutrient solution of this invention can provide good nutrients for crops, promote crop growth, and improve crop yield and quality.

[0043] Furthermore, the present invention provides the application of the aforementioned organic nutrient solution in the preparation of fertilizers or in promoting crop yield or improving crop quality.

[0044] Compared with the prior art, the beneficial effects of the present invention are as follows: This invention provides a convenient, cost-effective, and efficient organic nutrient solution made from pig manure, offering high-quality fertilizer resources for agriculture. The raw materials for this organic nutrient solution are widely available, and the fermentation cycle is short. It can increase soil organic matter content, improve soil health, thereby enhancing crop performance, promoting increased crop yield or quality, and is safe and environmentally friendly. It also reduces the use of chemical fertilizers and pesticides, lowering fertilization costs. This invention achieves efficient resource utilization of pig manure and provides a new technical path for the resource utilization and high-value fermentation of pig manure, with broad application prospects. Attached Figure Description

[0045] Figure 1 This is a process flow diagram of an embodiment of the present invention. Detailed Implementation

[0046] To make the objectives, technical solutions, and advantages of this invention clearer, the technical solutions of this invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of this invention, not all embodiments. In the embodiments provided in this specification, where specific techniques or conditions are not specified, they should be performed in accordance with the techniques or conditions described in the literature in this field, or in accordance with the product instructions. Reagents or instruments used without specified manufacturers are all conventional products that can be purchased through legitimate channels.

[0047] The process flow diagram of the following embodiments is as follows: Figure 1 As shown.

[0048] The EM bacterial agent used in the following examples is brand and catalog number Yifeipu Biotechnology 2025411; the compound Bacillus velezensis is a mixture of Bacillus velezensis BNCC352076 and Bacillus velezensis NM153 in a 1:1 mass ratio, wherein Bacillus velezensis NM153 is deposited at the China Center for Type Culture Collection, accession number CCTCC NO: M2025605, and the deposit date is March 27, 2025; Bacillus subtilis is BNCC336770; the compound streptococci are a mixture of Streptomyces flavus and Streptomyces freundii in a 1:1 mass ratio, wherein Streptomyces flavus was purchased from Haomeite 101595395703336; Streptomyces freundii was purchased from Weilaibo Technology (Beijing) Co., Ltd. BNCC336597.

[0049] The brands and product numbers of the synthetic enzymes are: protease (Victor WKQ-0387757), cellulase (Solebo C8270), and hemicellulase (Solebo H8110); the brands and product numbers of the mixed glycoenzymes are: glucosidase (Solebo P06941), pectinase (Solebo P8180), saccharifying enzyme (Yuanye S10017), and chitosanase (Solebo C6320); the mineral element powder was purchased from Inner Mongolia Lanjing Ecological Technology Co., Ltd.; the brand and product number of the seaweed extract is Ron R200892.

[0050] Example 1 This embodiment provides an organic nutrient solution (specifically for cash crops), prepared as follows: 1. Pretreatment and preparation of fermentation raw materials Carbon source auxiliary material: beet molasses and straw powder are mixed in a mass ratio of 4:1; Protein adjuvant: Mix commercially available soy protein powder and corn flour in a 2:1 mass ratio; EM bacterial agent has an effective live bacteria count ≥ 50 billion / g; Compound microbial inoculant: composed of compound Bacillus berleis, Bacillus subtilis and compound Streptococcus pyogenes in a mass ratio of 4:3:3; the viable count of compound Bacillus berleis ≥100 billion / g, the viable count of Bacillus subtilis ≥100 billion / g, and the viable count of compound Streptococcus pyogenes ≥20 billion / g.

[0051] The bioenzyme is a mixture of synthetic bioenzyme and mixed glycoenzyme in a mass ratio of 1:1; wherein, the synthetic bioenzyme is a mixture of protease, cellulase and hemicellulase in a mass ratio of 2:1:0.5, and the activity of each enzyme is ≥1000U / g; the mixed glycoenzyme is a mixture of glucase, pectinase, saccharifying enzyme and chitosanase in a mass ratio of 2:0.5:0.5:1, and the activity of each enzyme is ≥500U / g.

[0052] Mineral element powder: contains 30% (w / w) calcium, 10% (w / w) magnesium, 15% (w / w) sulfur, 15% (w / w) iron, 5% (w / w) copper, 10% (w / w) manganese, 8% (w / w) zinc, and 7% (w / w) boron.

[0053] Seaweed extract: contains 27% (w / w) seaweed polysaccharides.

[0054] 2. Preparation process flow Pig manure pretreatment—fermentation raw material preparation—micro-aerobic decomposition fermentation—facultative anaerobic synthesis fermentation—compound reduction preparation—3-stage filtration—bottling.

[0055] (1) Pretreatment of pig manure Filtration: The solid-liquid separated pig liquid manure (COD>5000mg / L) is filtered through a 20-mesh sieve and a 40-mesh sieve to remove large particulate impurities; Sterilization: The filtered pig liquid manure is sterilized with 1200 watts of ultraviolet light for 1 hour to obtain the manure fermentation stock solution to be prepared; (2) Fermentation raw material preparation Using C / N as the control parameters, and a filtered and sterilized mixture of pig liquid manure and urine as the main raw material, 800 kg of filtered and sterilized manure fermentation liquid, 100 kg of carbon source additives, and 40 kg of protein additives were taken. The final C / N ratio of the mixture was 25:1. The materials were mixed evenly and stirred to obtain the fermentation substrate. (3) Microaerobic decomposition fermentation Add 12 kg of EM agent, 10 kg of compound microbial agent, and 2 kg of biological enzyme to 1 ton of prepared fermentation substrate, stir and aerate for micro-aerobic decomposition fermentation, monitor the pH of the fermentation broth during fermentation, and when the pH of the fermentation broth drops below 5, the decomposition fermentation is completed and the primary fermentation broth is obtained.

[0056] Fermentation conditions: gas supply, stirring, and DO concentration in the fermentation broth maintained at 1.0–2.0 mg / L; Temperature: 27℃; Duration: 3 days.

[0057] (4) Anoxic Synthetic Fermentation Add 20 kg of protein adjuvant to each ton of primary fermentation liquid, stir and mix, then carry out facultative anaerobic synthesis fermentation and remove odors. Stop fermentation when the fermentation liquid turns golden yellow, has no obvious fecal odor, and has a distinct sweet and sour grain aroma.

[0058] Fermentation conditions: Open storage for fermentation, without gas supply or stirring; Temperature: 28℃; Duration: 3 days.

[0059] (5) Compound reduction blending and filtration bottling In the fermentation broth after facultative anaerobic synthesis fermentation, 6 kg of seaweed extract and 10 kg of mineral element powder are added per ton for compound reduction and blending. After being stirred and mixed in a reaction vessel, the mixture is then bottled after three-stage filtration.

[0060] Example 2 This embodiment provides an organic nutrient solution (specifically for field crops), prepared as follows: 1. Pretreatment and preparation of fermentation raw materials Carbon source auxiliary material: Sugarcane molasses and straw powder are mixed in a mass ratio of 4:1; Protein adjuvant: Mix commercially available soy protein powder and corn flour in a 2:1 mass ratio; EM bacterial agent has an effective live bacteria count ≥ 50 billion / g; Compound microbial inoculant: composed of compound Bacillus berleis, Bacillus subtilis and compound Streptococcus pyogenes in a mass ratio of 4:3:3; the viable count of compound Bacillus berleis ≥100 billion / g, the viable count of Bacillus subtilis ≥100 billion / g, and the viable count of compound Streptococcus pyogenes ≥20 billion / g.

[0061] The bioenzyme is a mixture of synthetic bioenzyme and mixed glycoenzyme in a mass ratio of 1:1; wherein, the synthetic bioenzyme is a mixture of protease, cellulase and hemicellulase in a mass ratio of 2:1:0.5, and the activity of each enzyme is ≥1000U / g; the mixed glycoenzyme is a mixture of glucase, pectinase, saccharifying enzyme and chitosanase in a mass ratio of 2:0.5:0.5:1, and the activity of each enzyme is ≥500U / g.

[0062] Mineral element powder: contains 30% (w / w) calcium, 10% (w / w) magnesium, 15% (w / w) sulfur, 15% (w / w) iron, 5% (w / w) copper, 10% (w / w) manganese, 8% (w / w) zinc, and 7% (w / w) boron.

[0063] Seaweed extract: contains 27% (w / w) seaweed polysaccharides.

[0064] 2. Preparation process flow Pig manure pretreatment—fermentation raw material preparation—micro-aerobic decomposition fermentation—facultative anaerobic synthesis fermentation—compound reduction preparation—3-stage filtration—bottling.

[0065] (1) Pretreatment of pig manure Filtration: The solid-liquid separated pig liquid manure (COD>5000mg / L) is filtered through a 20-mesh sieve and a 40-mesh sieve to remove large particulate impurities; Sterilization: The filtered pig liquid manure is sterilized with 1200 watts of ultraviolet light for 1 hour to obtain the manure fermentation stock solution to be prepared; (2) Fermentation raw material preparation Using C / N as the control parameters, and a filtered and sterilized mixture of pig liquid manure and urine as the main raw material, 900 kg of filtered and sterilized manure fermentation stock liquid, 50 kg of carbon source auxiliary material, and 20 kg of protein auxiliary material were taken. The final C / N ratio of the mixture was 22:1. The materials were mixed evenly and stirred to obtain the fermentation substrate. (3) Microaerobic decomposition fermentation Add 5 kg of EM agent, 5 kg of compound microbial agent, and 1 kg of biological enzyme to 1 ton of prepared fermentation substrate, stir and aerate for micro-aerobic decomposition fermentation, monitor the pH of the fermentation liquid during the fermentation process, and when the pH of the fermentation liquid drops below 5, the decomposition fermentation is completed and the primary fermentation liquid is obtained.

[0066] Fermentation conditions: gas supply, stirring, and DO concentration in the fermentation broth maintained at 1.0–2.0 mg / L; Temperature: 27℃; Duration: 3 days.

[0067] (4) Anoxic Synthetic Fermentation Add 10 kg of protein adjuvant to each ton of primary fermentation liquid, stir and mix, then carry out facultative anaerobic synthesis fermentation and remove odors. Stop fermentation when the fermentation liquid turns golden yellow, has no obvious fecal odor, and has a distinct sweet and sour grain aroma.

[0068] Fermentation conditions: Open storage for fermentation, without gas supply or stirring; Temperature: 28℃; Duration: 3 days.

[0069] (6) Compound reduction blending and filtration bottling In the fermentation broth after facultative anaerobic synthesis fermentation, 4 kg of seaweed extract and 5 kg of mineral element powder are added per ton for compound reduction and blending. After being stirred and mixed in a reaction vessel, the mixture is then bottled after three-stage filtration.

[0070] Example 3 This embodiment provides an organic nutrient solution (specifically for soil improvement), prepared as follows: 1. Pretreatment and preparation of fermentation raw materials Carbon source auxiliary material: Sugarcane molasses and straw powder are mixed in a mass ratio of 2:1; Protein adjuvant: Prepared by mixing commercially available soy protein powder and corn flour in a 1:1 mass ratio; EM bacterial agent has an effective live bacteria count ≥ 50 billion / g; Compound microbial inoculant: composed of compound Bacillus berleis, Bacillus subtilis and compound Streptococcus pyogenes in a mass ratio of 4:3:3; the viable count of compound Bacillus berleis ≥100 billion / g, the viable count of Bacillus subtilis ≥100 billion / g, and the viable count of compound Streptococcus pyogenes ≥20 billion / g.

[0071] The bioenzyme is a mixture of synthetic bioenzyme and mixed glycoenzyme in a mass ratio of 1:1; wherein, the synthetic bioenzyme is a mixture of protease, cellulase and hemicellulase in a mass ratio of 2:1:0.5, and the activity of each enzyme is ≥1000U / g; the mixed glycoenzyme is a mixture of glucase, pectinase, saccharifying enzyme and chitosanase in a mass ratio of 2:0.5:0.5:1, and the activity of each enzyme is ≥500U / g.

[0072] Mineral element powder: contains 30% (w / w) calcium, 10% (w / w) magnesium, 15% (w / w) sulfur, 15% (w / w) iron, 5% (w / w) copper, 10% (w / w) manganese, 8% (w / w) zinc, and 7% (w / w) boron.

[0073] Seaweed extract: contains 27% (w / w) seaweed polysaccharides.

[0074] 2. Preparation process flow Pig manure pretreatment—fermentation raw material preparation—micro-aerobic decomposition fermentation—facultative anaerobic synthesis fermentation—compound reduction preparation—3-stage filtration—bottling.

[0075] (1) Pretreatment of pig manure Filtration: The solid-liquid separated pig liquid manure (COD>5000mg / L) is filtered through a 20-mesh sieve and a 40-mesh sieve to remove large particulate impurities; Sterilization: The filtered pig liquid manure is sterilized with 1200 watts of ultraviolet light for 1 hour to obtain the manure fermentation stock solution to be prepared; (2) Fermentation raw material preparation Using C / N as the control parameters, and a filtered and sterilized mixture of pig liquid manure and urine as the main raw material, 950 kg of filtered and sterilized manure fermentation liquid, 30 kg of carbon source additives, and 5 kg of protein additives were taken. The final C / N ratio of the mixture was 20:1. The materials were mixed evenly and stirred to obtain the fermentation substrate. (3) Microaerobic decomposition fermentation Add 3 kg of EM inoculant, 3 kg of compound microbial inoculant, and 0.5 kg of biological enzyme to 1 ton of prepared fermentation substrate, stir and aerate for microaerobic decomposition fermentation, monitor the pH of the fermentation broth during fermentation, and when the pH of the fermentation broth drops below 5, the decomposition fermentation is complete and the primary fermentation broth is obtained.

[0076] Fermentation conditions: gas supply, stirring, and DO concentration in the fermentation broth maintained at 1.0–2.0 mg / L; Temperature: 27℃; Duration: 3 days.

[0077] (4) Anoxic Synthetic Fermentation Add 5 kg of protein adjuvant to each ton of primary fermentation liquid, stir and mix, then carry out facultative anaerobic synthesis fermentation and remove odors. Stop fermentation when the fermentation liquid turns golden yellow, has no obvious fecal odor, and has a distinct sweet and sour grain aroma.

[0078] Fermentation conditions: Open storage for fermentation, without gas supply or stirring; Temperature: 28℃; Duration: 3 days.

[0079] (7) Compound reduction blending and filtration bottling In the fermentation broth after facultative anaerobic synthesis fermentation, 1.5 kg of seaweed extract and 2 kg of mineral element powder are added per ton for compound reduction and blending. After being stirred and mixed in a reaction vessel, the mixture is then filtered through a three-stage filtration process and bottled.

[0080] Comparative Example 1 This comparative example provides an organic nutrient solution, the preparation method of which differs from that of Example 1 only in that: Replace the EM inoculant with an equal amount of lactic acid bacteria (purchased from Shandong Aikete Agricultural Development Co., Ltd., product number LS-RSJ-64875).

[0081] Comparative Example 2 This comparative example provides an organic nutrient solution, the preparation method of which differs from that of Example 1 only in that: Replace the compound Bacillus berberis in the compound microbial agent with an equal amount of Clostridium butyricum (purchased from Weilaibo Technology (Beijing) Co., Ltd., product number BNCC337239).

[0082] Comparative Example 3 This comparative example provides an organic nutrient solution, the preparation method of which differs from that of Example 1 only in that: Replace Bacillus subtilis in the compound microbial agent with an equal amount of Bacillus polymyxa (purchased from Weilaibo Technology (Beijing) Co., Ltd., product number BNCC337258).

[0083] Comparative Example 4 This comparative example provides an organic nutrient solution, the preparation method of which differs from that of Example 1 only in that: Replace the Streptomyces flavus in the compound streptococcus of the compound microbial agent with an equal amount of Streptomyces white (purchased from Weilaibo Technology (Beijing) Co., Ltd., item number BNCC186223).

[0084] Experimental Example 1 The pH value (representing the degree of fermentation), free amino acid concentration (representing the conversion efficiency), and effective viable cell count (representing the functional microbial culture and propagation effect) of the organic nutrient solutions prepared in the above examples and comparative examples were tested. The pH test method was based on HJ 1147-2020. The free amino acid test method was based on NY / T 1975-2010. The effective viable cell count test method was based on NY / T 2321-2013.

[0085] The test results are shown in Table 1.

[0086] Table 1

[0087] Experimental Example 2 This experiment demonstrates the fertilizer effectiveness; the steps are as follows: The high-efficiency liquid organic nutrient solution prepared in Examples 1-3 was used in a cotton planting experiment at a cotton planting base of a Xinjiang Production and Construction Corps in Hami City. The cotton variety was Minfeng 9. Four treatments were set up in the experiment. The experimental groups and fertilization treatments are shown in Table 2. The control group received conventional chemical fertilizers from local farmers, while the experimental groups received the organic nutrient solution provided in Examples 1-3 of this invention in addition to the chemical fertilizers, with a 50% reduction in chemical fertilizer usage. The organic fertilizer used as base fertilizer was a commercially available organic fertilizer with ≥30% organic matter and ≥4% NPK. The chemical fertilizer used as base fertilizer was a 35% (N14-P2O514-K2O7) compound fertilizer, and the top dressing was 46% water-soluble urea. Each plot was 1 mu (approximately 0.067 hectares), planted in 6 rows with one film covering, with a row spacing of (10+60+10+60+10) cm and a plant spacing of 10 cm. Drip irrigation under the film and dry sowing with wet emergence techniques were used.

[0088] Table 2 Fertilization details for different treatment groups

[0089] The basic physical and chemical properties of the test soil are shown in Table 3.

[0090] Table 3 Soil physicochemical properties

[0091] Cotton yield and quality were measured at the time of cotton harvest, as shown in Table 4.

[0092] Table 4. Experimental cotton yield and quality

[0093] As can be seen from Table 4 above, the organic nutrient solution prepared in this embodiment of the invention still produces a significant yield increase for cotton while reducing the use of conventional chemical fertilizers by 20%, with a yield increase of 3.50%-11.32% compared to the control group of commercially available conventional fertilizers. This is because the organic nutrient solution prepared in this invention uses an innovative fermentation process to convert organic matter and protein in pig manure into amino acids, short-chain fatty acids, and other substances that are easily absorbed by plants and have significant effects on promoting growth, increasing yield, and improving quality. At the same time, the fermentation process promotes the growth and proliferation of functional microorganisms, increases the accumulation of beneficial metabolites in the organic nutrient solution, and, in conjunction with the addition of mineral elements and seaweed extracts, promotes the improvement of cotton yield and quality.

[0094] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. An organic nutrient solution, characterized in that, Its preparation methods include: (1) Using sterilized pig manure as raw material, carbon source and nitrogen source are added, and then EM agent, compound microbial agent and biological enzyme are introduced for micro-aerobic decomposition and fermentation to obtain primary fermentation liquid; the compound microbial agent is composed of compound Bacillus belye, Bacillus subtilis and compound streptococcus; the compound streptococcus is composed of Streptomyces flavus and Streptomyces freundii. (2) Add a nitrogen source to the primary fermentation broth and carry out facultative anaerobic synthetic fermentation to obtain a secondary fermentation broth; (3) The organic nutrient solution is prepared by adding plant growth promoter to the secondary fermentation liquid.

2. The organic nutrient solution as described in claim 1, characterized in that, The mass ratio of the compound Bacillus belyssus, Bacillus subtilis and compound streptococcus was (1~2):(0.8~1.2):

1.

3. The organic nutrient solution as described in claim 1 or 2, characterized in that, The composite Bacillus berlesi consists of Bacillus berlesi BNCC352076 and Bacillus berlesi NM153. And / or, the Bacillus subtilis is Bacillus subtilis BNCC336770; And / or, the pig manure is liquid pig manure after solid-liquid separation; And / or, the sterilization is performed using 1200~1500 watt ultraviolet light.

4. The organic nutrient solution as described in claim 1, characterized in that, The carbon source includes at least one of straw powder, starch, molasses, glucose, and brown sugar; And / or, the nitrogen source includes at least one of soybean protein powder and corn protein powder.

5. The organic nutrient solution as described in claim 1 or 2, characterized in that, The bioenzymes include synthetic bioenzymes and mixed glycoenzymes; The synthetic biological enzymes include proteases, cellulases, and hemicellulases; The mixed glycoenzymes include glucosidase, pectinase, saccharifying enzyme, and chitosanase; And / or, the plant promoters include seaweed extracts and mineral elements.

6. The organic nutrient solution as described in claim 5, characterized in that, The bioenzyme comprises synthetic bioenzymes and mixed glycoenzymes in a mass ratio of 1:(0.8~1.2); In the synthesized biological enzyme, the mass ratio of protease, cellulase, and hemicellulase is (1.5~2.5):1:(0.3~0.7). In the mixed glycoenzymes, the mass ratio of glucosidase, pectinase, saccharifying enzyme and chitosanase is (1.5~2.5):(0.3~0.7):(0.3~0.7):

1.

7. The organic nutrient solution as described in claim 1 or 2, characterized in that, In the substrate of the microaerobic decomposition fermentation, the C / N ratio is (20~30):1; in the material of the microaerobic decomposition fermentation, the mass ratio of EM inoculant, compound microbial inoculant and biological enzyme is (5~20):(5~15):

1.

8. The organic nutrient solution as described in claim 1, characterized in that, The temperature for microaerobic decomposition fermentation is 5℃~40℃; the conditions for microaerobic decomposition fermentation include: gas supply, stirring, and maintaining the DO in the fermentation broth at 1.0~2.0 mg / L; the endpoint of microaerobic decomposition fermentation is a pH below 5.

9. The organic nutrient solution as described in claim 1, characterized in that, Add 5-30 kg of nitrogen source to each ton of the primary fermentation liquid and then carry out anaerobic synthesis fermentation; the temperature of the anaerobic synthesis fermentation is 5℃-40℃; the conditions for anaerobic synthesis fermentation are open storage fermentation without gas supply or stirring; the endpoint of the anaerobic synthesis fermentation is that the fermentation liquid turns golden yellow, has no obvious fecal odor, and has a relatively obvious sweet and sour grain aroma.

10. The use of the organic nutrient solution according to any one of claims 1 to 9 in the preparation of fertilizers or in promoting crop yield or improving crop quality. Preferably, the crop is cotton.

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